Consulta: subjectFacets:"Microbial communities"
Registros recuperados: 21
Data/hora: 08/06/2024 19:10:53

Diversity of bacterial communities in acid mine drainage from the Shen-bu copper mine, Gansu province, China
Provedor de dados: 69
Autores: Yang,Yu; Shi,Wuyang; Wan,Minxi; Zhang,Yanfei; Zou,Lihong; Huang,Jufang; Qiu,Guanzhou; Liu,Xueduan.
This study presents bacterial population analyses of microbial communities inhabiting three sites of acid mine drainage (AMD) in the Shen-bu copper mine, Gansu Province, China. These sites were located next to acid-leached chalcopyrite slagheaps that had been abandoned since 1995. The pH values of these samples with high concentrations of metals ranged from 2.0 to 3.5. Amplified ribosomal DNA restriction analysis (ARDRA) was used to characterize the bacterial population by amplifying the 16S rRNA gene of microorganisms. A total of 39 operational taxonomic units (OTUs) were obtained from the three samples and sequenced from 384 clones. Sequence data and phylogenetic analyses showed that two dominant clones (JYC-1B, JYC-1D) in sample JYC-1 represented 69.5%...
Tipo: Journal article
Palavras-chave: Acid mine drainage; Amplified ribosomal DNA restriction analysis; Microbial communities.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100001

Sistemas de cultivo utilizados na cultura da soja e efeito sobre a comunidade microbiana do solo.
Provedor de dados: 14
Autores: DIAS, L. A. F.; LOPES, I. de O. N.; CATTELAN, A. J.; DEBIASI, H.; SIBALDELLI, R. N. R.; KOSINSK, C. L.; SEIXAS, C. D. S.; ALMEIDA, A. M. R.
A rotação de culturas é estratégia importante no controle de insetos-pragas e doenças. No entanto, a dimensão de sua efetividade depende da duração da rotação e/ou do histórico do solo, das culturas implantadas no sistema, bem como a sequência e a frequência das mesmas. Este trabalho relata os efeitos de diferentes sistemas de rotação utilizando soja, milho, milho safrinha, tremoço-branco, ervilha-forrageira, trigo e aveia sobre a comunidade microbiana dos solos em sistema de plantio direto. Além desses tratamentos, foram considerados dois tratamentos testemunha: monocultivo de trigo e monocultivo de aveia, em sistema de plantio convencional. As amostras de solo foram coletadas em ensaio de rotação de culturas iniciado em 1989, tendo sido as avaliações...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Soja; Rotação de Cultura; Bactéria; Fungo; Soybeans; Cropping systems; Microbial communities.
Ano: 2018
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1100651

Archaeal communities associated with shallow to deep subseafloor sediments of the New Caledonia Basin
Provedor de dados: 5
Autores: Roussel, Erwan; Sauvadet, Anne-laure; Chaduteau, Carine; Fouquet, Yves; Charlou, Jean-luc; Prieur, Daniel; Cambon, Marie-anne.
P&gt;The distribution of the archaeal communities in deep subseafloor sediments [0-36 m below the seafloor (mbsf)] from the New Caledonia and Fairway Basins was investigated using DNA- and RNA-derived 16S rRNA clone libraries, functional genes and denaturing gradient gel electrophoresis (DGGE). A new method, Co-Migration DGGE (CM-DGGE), was developed to access selectively the active archaeal diversity. Prokaryotic cell abundances at the open-ocean sites were on average similar to 3.5 times lower than at a site under terrestrial influence. The sediment surface archaeal community (0-1.5 mbsf) was characterized by active Marine Group 1 (MG-1) Archaea that co-occurred with ammonia monooxygenase gene (amoA) sequences affiliated to a group of uncultured...
Tipo: Text
Palavras-chave: Marine subsurface sediments; Sea floor biosphere; Gradient gel electrophoresis; Microbial communities; Molecular diversity; Anaerobic oxidation; Sequence alignment; Phylogenetic trees; Extracellular DNA; Methane hydrate.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6801.pdf

Etude bactériologique des sédiments abyssaux. Etude de la dynamique des communautés microbiennes et de leurs activités hétérotrophes dans les parcs conchylicoles
Provedor de dados: 5
Autores: Bianchi, A.
Bacteriological study of abyssal sediments: During the Demeraby mission of the N/O Jean Charcot in the western Atlantic Ocean, a study of the microflora of the digestive tracts of echinoderms (Holothuroidea of various genera, Asteroidea, Echinoidea) was compared with that of the surrounding sediment.  The bacterial concentration observed in the digestive tracts of all the animals studied is higher than that in the surrounding sediments. The largest numbers were always found in the most anterior part of the digestive tracts of the holothurians. In some cases, the increase is considerable (7.10 versus 5.2. 10 cells per millilitre): It indicates a proliferation of bacterial cells in the anterior part of the digestive tract. As the sediment moves through the...
Tipo: Text
Palavras-chave: Conchylaceous parks; Microbial communities; Abyssal sediments; Bacteriological study.
Ano: 1981
URL: http://archimer.ifremer.fr/doc/1981/rapport-1926.pdf

Chronic Polyaromatic Hydrocarbon (PAH) Contamination Is a Marginal Driver for Community Diversity and Prokaryotic Predicted Functioning in Coastal Sediments
Provedor de dados: 5
Autores: Jeanbille, Mathilde; Gury, Jérôme; Duran, Robert; Tronczynski, Jacek; Ghiglione, Jean-françois; Agogué, Hélène; Saïd, Olfa Ben; Taïb, Najwa; Debroas, Didier; Garnier, Cédric; Auguet, Jean-christophe.
Benthic microorganisms are key players in the recycling of organic matter and recalcitrant compounds such as polyaromatic hydrocarbons (PAHs) in coastal sediments. Despite their ecological importance, the response of microbial communities to chronic PAH pollution, one of the major threats to coastal ecosystems, has received very little attention. In one of the largest surveys performed so far on coastal sediments, the diversity and composition of microbial communities inhabiting both chronically contaminated and non-contaminated coastal sediments were investigated using high-throughput sequencing on the 18S and 16S rRNA genes. Prokaryotic alpha-diversity showed significant association with salinity, temperature, and organic carbon content. The effect of...
Tipo: Text
Palavras-chave: Microbial communities; PAH; Chronic contamination; Coastal sediment; Functional diversity.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00347/45854/45522.pdf

Microbial diversity in a submarine carbonate edifice from the serpentinizing hydrothermal system of the Prony Bay (New Caledonia) over a 6-year period
Provedor de dados: 5
Autores: Postec, Anne; Quemeneur, Marianne; Bes, Meline; Mei, Nan; Benaissa, Fatma; Payri, Claude; Pelletier, Bernard; Monnin, Christophe; Guentas-dombrowsky, Linda; Ollivier, Bernard; Gerard, Emmanuelle; Pisapia, Celine; Gerard, Martine; Menez, Benedicte; Erauso, Gael.
Active carbonate chimneys from the shallow marine serpentinizing Prony Hydrothermal Field were sampled 3 times over a 6 years period at site ST09. Archaeal and bacterial communities composition was investigated using PCR-based methods (clone libraries, Denaturating Gel Gradient Electrophoresis, quantitative PCR) targeting 16S rRNA genes, methyl coenzyme M reductase A and dissimilatory sulfite reductase subunit B genes. Methanosarcinales (Euryarchaeota) and Thaumarchaea were the main archaeal members. The Methanosarcinales, also observed by epifluorescent microscopy and FISH, consisted of two phylotypes that were previously solely detected in two other serpentinitzing ecosystems (The Cedars and Lost City Hydrothermal Field). Surprisingly, members of the...
Tipo: Text
Palavras-chave: Hydrothermal; Serpentinization; Alkaline; Prony; Microbial communities.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00467/57916/60307.pdf

Sustaining Rare Marine Microorganisms: Macroorganisms As Repositories and Dispersal Agents of Microbial Diversity
Provedor de dados: 5
Autores: Troussellier, Marc; Escalas, Arthur; Bouvier, Thierry; Mouillot, David.
Recent analyses revealed that most of the biodiversity observed in marine microbial communities is represented by organisms with low abundance but, nonetheless essential for ecosystem dynamics and processes across both temporal and spatial scales. Surprisingly, few studies have considered the effect of macroorganism-microbe interactions on the ecology and distribution dynamics of rare microbial taxa. In this review, we synthesize several lines of evidence that these relationships cannot be neglected any longer. First, we provide empirical support that the microbiota of macroorganisms represents a significant part of marine bacterial biodiversity and that host-microbe interactions benefit to certain microbial populations which are part of the rare biosphere...
Tipo: Text
Palavras-chave: Microbial communities; Microbial biodiversity; Rare biosphere; Microbiota; Macroorganism-microbe interactions; Dispersal; Metacommunity; Gut microbiota.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00390/50123/50721.pdf

Mats of psychrophilic thiotrophic bacteria associated with cold seeps of the Barents Sea
Provedor de dados: 5
Autores: Gruenke, S.; Lichtschlag, A.; De Beer, D.; Felden, J.; Salman, V.; Ramette, A.; Schulz-vogt, H. N.; Boetius, A.
This study investigated the bacterial diversity associated with microbial mats of polar deep-sea cold seeps. The mats were associated with high upward fluxes of sulfide produced by anaerobic oxidation of methane, and grew at temperatures close to the freezing point of seawater. They ranged from small patches of 0.2-5 m in diameter (gray mats) to extensive fields covering up to 850 m(2) of seafloor (white mats) and were formed by diverse sulfide-oxidizing bacteria differing in color and size. Overall, both the dominant mat-forming thiotrophs as well as the associated bacterial communities inhabiting the mats differed in composition for each mat type as determined by microscopy, 16S rRNA gene sequencing and automated ribosomal intergenic spacer analysis....
Tipo: Text
Palavras-chave: Mosby mud volcano; Gulf of mexico; Eastern mediterranean sea; Filamentous sulfur bacteria; Sulfide oxidizing bacteria; In situ hybridization; Sulfate reduction; Microbial communities; Marine sediments; Beggiatoa spp.
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00212/32360/30823.pdf

Dynamique spatiale et temporelle des communautés microbiennes dans les édifices hydrothermaux actifs
Provedor de dados: 5
Autores: Francois, David.
In deep-sea hydrothermal vents, the mixing between hot, acidic and anoxic hydrothermal fluid with seawater leads to the formation of hydrothermal chimneys. These are porous structures whose mineralogical composition changes over time. Thermal and chemical gradients are favourable to the growth of microbial communities, that present a high taxonomic, physiologic and metabolic diversity. Despite numerous studies about the microbiology of hydrothermal environments, the spatial and temporal dynamics of microbial communities in hydrothermal vents remain relatively unknown. Our study was based on a rigorous sampling of hydrothermal chimneys from six sites in the Lucky Strike hydrothermal vent field, and the associated proximal plume. The mineralogical...
Tipo: Text
Palavras-chave: Sources hydrothermales; Communautés microbiennes; Persephonella; Hydrothermal vents; Microbial communities; Persephonella.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00690/80209/83298.pdf

Iron oxide deposits associated with the ectosymbiotic bacteria in the hydrothermal vent shrimp Rimicaris exoculata
Provedor de dados: 5
Autores: Corbari, L; Cambon-bonavita, Marie-anne; Long, G; Grandjean, F; Zbinden, M; Gaill, F; Compere, P.
The Rimicaris exoculata shrimp is considered as a primary consumer that dominates the fauna of most Mid-Atlantic Ridge ( MAR) hydrothermal ecosystems. These shrimps harbour in their gill chambers an important ectosymbiotic community of chemoautotrophic bacteria associated with iron oxide deposits. The structure and elemental composition of the mineral concretions associated with these bacteria have been investigated by using LM, ESEM, TEM STEM and EDX microanalyses. The nature of the iron oxides in shrimps obtained from the Rainbow vent field has also been determined by Mossbauer spectroscopy. This multidisciplinary approach has revealed that the three layers of mineral crust in the Rimicaris exoculata shrimps consist of large concretions formed by...
Tipo: Text
Palavras-chave: Mid atlantic ridge; Deep sea; Microbial communities; Oxidizing bacteria; Trophic ecology; Ferrous ions; Fe oxides; Ferrihydrite; Biomineralization; Minerals.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4702.pdf

Identification des communautés microbiennes des lobes terminaux du système turbiditique du Congo
Provedor de dados: 5
Autores: Bessette, Sandrine.
The Congo deep sea fan, located in the Congo-Angola continental margin (West African coast, Equatorial South Atlantic Ocean) represents a unique deep-sea sedimentary ecosystem. It is characterized by high organic matter inputs from the Congo River, that flow along a canyon and through presently active channel system-lifted into the deeper areas (5 000 m) where the lobes system develops.The aim of this thesis is to study the spatial distribution as well as the phylogenetic and functional diversity of archaeal and bacterial communities in relation with environmental characteristics and constraints of the terminal lobes of the Congo deep see fan, one of the largest submarine fan systems in the world.This study highlights geographical distribution of microbial...
Tipo: Text
Palavras-chave: Système turbiditique du Congo; Sédiment riche en matière organique; Communautés microbiennes; Bactéries méthanotrophes aérobies; Suintements de méthane; Methane seeps; Congo turbiditic system; Organic-rich sediment; Microbial communities; Aerobic methane oxidizing bacteria.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00498/60929/64323.pdf

Diversité phylogénétique et fonctionnelle des communautés microbiennes incultivées des sédiments marins de la marge de Sonora, Bassin de Guaymas (Golfe de Californie)
Provedor de dados: 5
Autores: Vigneron, Adrien.
At continental margins, and more particularly in cold seep areas, microbial and animal communities were locally detected at the surface of the sediments. These communities grow using reduced chemical compounds (H2S, Methane, COZ ...) contained in the percolated cold fluids and produced by both geological and microbial processes. ln order to study microbial community diversity in these ecosystems and their role in the environment as well as to understand the environmental factors influencing the distribution and ecophysiology of these communities, surface (0-20 cmbsf) but also deeper (&lt;9 mbsf) sediments were collected at the Sonora Margin. Microbial communities have been studied using various molecular, cultural and microscopy approaches. This research...
Tipo: Text
Palavras-chave: Fluides froids; Marge de Sonora; Bassin de Guaymas; Diversité moléculaire; Communautés microbiennes; Archaea; Bacteria; Méthanogènes; Methanogens; Microbial communities; Molecular diversity; Guaymas Basin; Sonora Margin; ANME; SRb; Methane; Sediments; Cold seeps.
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00498/60956/64359.pdf

Microbial functional diversity: From concepts to applications
Provedor de dados: 5
Autores: Escalas, Arthur; Hale, Lauren; Voordeckers, James W.; Yang, Yunfeng; Firestone, Mary K.; Alvarez‐cohen, Lisa; Zhou, Jizhong.
Functional diversity is increasingly recognized by microbial ecologists as the essential link between biodiversity patterns and ecosystem functioning, determining the trophic relationships and interactions between microorganisms, their participation in biogeochemical cycles, and their responses to environmental changes. Consequently, its definition and quantification have practical and theoretical implications. In this opinion paper, we present a synthesis on the concept of microbial functional diversity from its definition to its application. Initially, we revisit to the original definition of functional diversity, highlighting two fundamental aspects, the ecological unit under study and the functional traits used to characterize it. Then, we discuss how...
Tipo: Text
Palavras-chave: Functional diversity; Functional traits; Microbial communities; Theoretical frameworks of diversity; Trait-based ecology.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00584/69631/67487.pdf

Biodiversity of Cold Seep Ecosystems Along the European Margins
Provedor de dados: 5
Autores: Vanreusel, Ann; Andersen, Ann C.; Boetius, Antje; Connelly, Douglas; Cunha, Marina R.; Decker, Carole; Hilario, Ana; Kormas, Konstantinos Ar.; Maignien, Lois; Olu, Karine; Pachiadaki, Maria; Ritt, Benedicte; Rodrigues, Clara; Sarrazin, Jozee; Tyler, Paul; Van Gaever, Saskia; Vanneste, Heleen.
During the European Commission's Framework Six Programme, HERMES, we investigated three main areas along the European margin, each characterized by the presence of seep-related structures exhibiting different intensity of activity and biological diversity. These areas are: (1) the Nordic margin with the Hakon Mosby mud volcano and many pockmarks, (2) the Gulf of Cadiz, and (3) the eastern Mediterranean with its hundreds of mud volcanoes and brine pool structures. One of the main goals of the HERMES project was to unravel the biodiversity associated with these seep-associated ecosystems, and to understand their driving forces and functions, using an integrated approach. Several multidisciplinary research cruises to these three areas provided evidence of...
Tipo: Text
Palavras-chave: Mosby mud volcano; Gulf of mexico; Cadiz ne atlantic; Deep sea fan; Community structure; Microbial communities; Fluid flow; Mediterranean sea; Carbonate crusts; Norwegian sea.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6375.pdf

Microbial communities associated with benthic faunal assemblages at cold seep sediments of the Sonora Margin, Guaymas Basin
Provedor de dados: 5
Autores: Cruaud, Perrine; Vigneron, Adrien; Pignet, Patricia; Caprais, Jean-claude; Lesongeur, Francoise; Toffin, Laurent; Godfroy, Anne; Cambon-bonavita, Marie-anne.
The Sonora Margin cold seeps present a seafloor mosaic pattern consisting of different faunal assemblages and microbial mats. To better understand if sedimentary microbial communities reflect this patchy distribution, all major habitats were investigated using four complementary approaches: 16S rRNA gene sequence 454 pyrosequencing, quantitative polymerase chain reaction, fluorescence in situ hybridization and geochemistry analyses. This study reveals that sediments populated by different surface assemblages show distinct porewater geochemistry features and are associated with distinct microbial communities. In the sediments underlying the microbial mat and the surrounding macrofauna, microbial communities were dominated by anaerobic methane oxidizers...
Tipo: Text
Palavras-chave: Microbial communities; Pyrosequencing; MBG-D; ANME; Faunal assemblages; Vesicomyidae; Siboglinidae; Microbial mat.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00288/39889/38434.pdf

Bacterial diversity of soil under eucalyptus assessed by 16S rDNA sequencing analysis
Provedor de dados: 86
Autores: Silveira,Érico Leandro da; Pereira,Rodrigo Matheus; Scaquitto,Denilson César; Pedrinho,Eliamar Aparecida Nascimbém; Val-Moraes,Silvana Pómpeia; Wickert,Ester; Carareto-Alves,Lúcia Maria; Lemos,Eliana Gertrudes de Macedo.
Studies on the impact of Eucalyptus spp. on Brazilian soils have focused on soil chemical properties and isolating interesting microbial organisms. Few studies have focused on microbial diversity and ecology in Brazil due to limited coverage of traditional cultivation and isolation methods. Molecular microbial ecology methods based on PCR amplified 16S rDNA have enriched the knowledge of soils microbial biodiversity. The objective of this work was to compare and estimate the bacterial diversity of sympatric communities within soils from two areas, a native forest (NFA) and an eucalyptus arboretum (EAA). PCR primers, whose target soil metagenomic 16S rDNA were used to amplify soil DNA, were cloned using pGEM-T and sequenced to determine bacterial diversity....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Metagenomic; Microbial communities.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006001000008

Exploring interactions of plant microbiomes
Provedor de dados: 63
Autores: Andreote,Fernando Dini; Gumiere,Thiago; Durrer,Ademir.
A plethora of microbial cells is present in every gram of soil, and microbes are found extensively in plant and animal tissues. The mechanisms governed by microorganisms in the regulation of physiological processes of their hosts have been extensively studied in the light of recent findings on microbiomes. In plants, the components of these microbiomes may form distinct communities, such as those inhabiting the plant rhizosphere, the endosphere and the phyllosphere. In each of these niches, the "microbial tissue" is established by, and responds to, specific selective pressures. Although there is no clear picture of the overall role of the plant microbiome, there is substantial evidence that these communities are involved in disease control, enhance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbial communities; Rhizosphere; Endophytes; Phyllosphere.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162014000600013

Comparing how land use change impacts soil microbial catabolic respiration in Southwestern Amazon
Provedor de dados: 58
Autores: Mazzetto,Andre Mancebo; Feigl,Brigitte Josefine; Cerri,Carlos Eduardo Pellegrino; Cerri,Carlos Clemente.
Abstract Land use changes strongly impact soil functions, particularly microbial biomass diversity and activity. We hypothesized that the catabolic respiration response of the microbial biomass would differ depending on land use and that these differences would be consistent at the landscape scale. In the present study, we analyzed the catabolic response profile of the soil microbial biomass through substrate-induced respiration in different land uses over a wide geographical range in Mato Grosso and Rondônia state (Southwest Amazon region). We analyzed the differences among native areas, pastures and crop areas and within each land use and examined only native areas (Forest, Dense Cerrado and Cerrado), pastures (Nominal, Degraded and Improved) and crop...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazon region; Land use change; Catabolic profile; Microbial communities.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100063

Cultivated bacterial diversity associated with the carnivorous plant Utricularia breviscapa (Lentibulariaceae) from floodplains in Brazil
Provedor de dados: 58
Autores: Lima,Felipe Rezende; Ferreira,Almir José; Menezes,Cristine Gobbo; Miranda,Vitor Fernandes Oliveira; Dourado,Manuella Nóbrega; Araújo,Welington Luiz.
ABSTRACT Carnivorous plant species, such as Utricularia spp., capture and digest prey. This digestion can occur through the secretion of plant digestive enzymes and/or by bacterial digestive enzymes. To comprehend the physiological mechanisms of carnivorous plants, it is essential to understand the microbial diversity related to these plants. Therefore, in the present study, we isolated and classified bacteria from different organs of Utricularia breviscapa (stolons and utricles) and from different geographic locations (São Paulo and Mato Grosso). We were able to build the first bacterium collection for U. breviscapa and study the diversity of cultivable bacteria. The results show that U. breviscapa bacterial diversity varied according to the geographic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Utricularia breviscapa; Microbial ecology; Aquatic microbiota; Microbial communities.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400714

An optmized protocol for simultaneous extraction of DNA and RNA from soils
Provedor de dados: 58
Autores: Costa,Rodrigo; Gomes,Newton C.M.; Milling,Annett; Smalla,Kornelia.
In this work we report an optimized protocol for simultaneous extraction of DNA and RNA from soil matrices. Treatment of soil matrices with ethanol followed by bead-beating worked as a successful strategy to lyse the cells without considerable degradation of nucleic acids, resulting in DNA and RNA of good yield and integrity. The reverse transcribed RNA could be amplified with primers targeting a glutamine synthetase (glnA) gene fragment. From both DNA and cDNA, 16S rDNA fragments were amplified and analyzed by Denaturing Gradient Gel Electrophoresis (DGGE). The method was applied to soil and rhizosphere (strawberry and oilseed rape) samples. Two other protocols for the extraction of nucleic acids from soil were applied to the same set of samples in order...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNA extraction; Nucleic acids; Rhizosphere; Microbial communities.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200011

Comparison of DNA extraction protocols for microbial communities from soil treated with biochar
Provedor de dados: 58
Autores: Leite,D.C.A.; Balieiro,F.C.; Pires,C.A.; Madari,B.E.; Rosado,A.S.; Coutinho,H.L.C.; Peixoto,R.S.
Many studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high quality microbial DNA from biochar-amended soil is not a trivial process and can be considerably more difficult than the extraction of DNA from other environmental samples. The aim of this study was to compare the relative efficacies of three commercial DNA extraction...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Biochar; DNA extraction; PCR-DGGE; Microbial communities; DNA purity indices.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100023