Consulta: subjectFacets:"Microbial control"
Registros recuperados: 27
Data/hora: 09/06/2024 05:25:00

Natural Occurrence of the Entomopathogenic Fungi Metarhizium, Beauveria and Paecilomyces in Soybean Under Till and No-till Cultivation Systems
Provedor de dados: Neotropical Entomology
Autores: SOSA-GÓMEZ,DANIEL R.; DELPIN,KATIAÍRES E.; MOSCARDI,FLÁVIO; FARIAS,JOSÉ R. B.
The occurrence of the entomopathogenic fungi Metarhizium, Beauveria and Paecilomyces in soybean under till and no-till cultivation systems was studied. The number of colony forming units per g of soil and per cm² of leaf area was determined. Soil under no-till system showed a higher incidence of entomopathogenic fungi, but no difference in fungal density was observed on leaves. Thus the probability of infection for susceptible leaf inhabiting insects should be similar in both systems.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epizootiology; Deuteromycetes; Moniliaceae; Microbial control.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2001000300011

Cage and field assessments of Beauveria bassiana-based Mycoinsecticides for Myzus persicae Sulzer (Hemiptera: Aphididae) control in cabbage
Provedor de dados: Neotropical Entomology
Autores: Michereff Filho,M; Oliveira,SOD; de Liz,RS; Faria,M.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Entomopathogenic fungus; Formulation; Microbial control.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2011000400010

Differential susceptibility of adults and nymphs of Blattella germanica (L.) (Blattodea: Blattellidae) to infection by Metarhizium anisopliae and assessment of delivery strategies
Provedor de dados: Neotropical Entomology
Autores: Lopes,RB; Alves,SB.
Microbial insecticides for cockroach control, such as those containing entomopathogenic fungi, may be an alternative to reduce contamination by chemicals in housing and food storage environments. Virulence of isolate ESALQ1037 belonging to the Metarhizium anisopliae complex against nymphs and adults of Blattella germanica (L.), and its infectivity following exposure of insects to a contaminated surface or to M. anisopliae-bait were determined under laboratory conditions. Estimated LD50 15 d following topical inoculation was 2.69 x 10(5) conidia per adult, whereas for nymphs the maximum mortality was lower than 50%. Baits amended with M. anisopliae conidia had no repellent effect on targets; adult mortality was inferior to 25%, and nymphs were not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: German cockroach; Microbial control; Entomopathogenic fungi.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2011000300010

Effect of Thiamethoxam on Entomopathogenic Microorganisms
Provedor de dados: Neotropical Entomology
Autores: BATISTA FILHO,ANTONIO; ALMEIDA,JOSÉ E.M.; LAMAS,CLÓVIS.
The compatibility of entomopathogenic microorganisms with thiamethoxam and other insecticides was studied in vitro and under field conditions. The microorganisms tested were: Aschersonia aleyrodis, Bacillus thuringiensis, Baculovirus anticarsia (NPVAg), Beauveria bassiana, Hirsutella thompsonii, Metarhizium anisopliae, Nomuraea rileyi, Paecilomyces farinosus, Sporothrix insectorum and Verticillium lecanii. Two concentrations of each product were tested in the laboratory, based on the maximum and minimum recommended rates for application in the field. The products were added to specific culture medium for growth of the entomopathogens. Reproductive and vegetative growth were evaluated for fungi, and colony forming units (CFU) were evaluated for bacteria....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Insecticide; Microbial control; Compatibility.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2001000300017

Pathogenicity of Metarhizium anisopliae for Ceratitis capitata (Wied.) (Diptera: Tephritidae) in soil with different pesticides
Provedor de dados: Neotropical Entomology
Autores: Mochi,Dinalva A.; Monteiro,Antonio C.; De Bortoli,Sergio A.; Dória,Háyda O.S.; Barbosa,José C.
This research intended to investigate if the presence of pesticides in the soil could affect the pathogenicity of Metarhizium anisopliae Metsch. (Sorokin) for Ceratitis capitata (Wied.) and assess the effect of conidia application as suspension or dry conidia. The fungicides chlorothalonyl and tebuconazol, the acaricide abamectin, the insecticide trichlorfon, and the herbicide ametrin were applied at the manufacturer-recommended doses. Soil samples were placed in glass flasks and were given the fungus as conidial suspension or dry. After pesticide application, 20 3rd-instar larvae were placed in the soil. The flasks were sealed with voile fabric and incubated at 27 ± 0.5ºC for nine days, until adult emergence; incubation continued for four more days at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Microbial control; Entomopathogenic fungus; Fruit fly.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000300014

Influence of soil temperature and moisture on the infectivity of entomopathogenic nematodes (Rhabditida: Heterorhabditidae, Steinernematidae) against larvae of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)
Provedor de dados: Neotropical Entomology
Autores: Rohde,Cristhiane; Moino Jr,Alcides; Silva,Marco A T da; Carvalho,Fabiano D; Ferreira,Cleidson S.
The Mediterranean fruit fly, Ceratitis capitata (Wiedemann), is considered one of the main pests that affect fruit production in the world. This insect spends part of its life cycle in the soil, making it a target for entomopathogenic nematodes. This work aimed at evaluating the influence of soil temperature and moisture on the infectivity of Heterorhabditis sp. RSC01 and Steinernema carpocapsae ALL to third-instars of C. capitata, and to compare the efficiency of these isolates at five different soil temperatures (19, 22, 25, 28, and 31°C) and three levels of relative soil moisture (100, 75, and 50% of field capacity). Ten C. capitata larvae were transferred to plastic jars (12 cm × 6 cm) containing 100 g soil, followed by the application of an aqueous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbial control; Fruit fly; Abiotic factor.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000400022

External events related to the infection process of Cornitermes cumulans (Kollar) (Isoptera: Termitidae) by the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae
Provedor de dados: Neotropical Entomology
Autores: Neves,Pedro M.O.J.; Alves,Sérgio B.
The objectives of this study were to observe the external development of Metarhizium anisopliae and Beauveria bassiana on workers and soldiers of Cornitermes cumulans (Kollar) and to establish comparisons between the insect fixation techniques known as critical point and desiccator. Termite workers and soldiers inoculated with B. bassiana (447) and M. anisopliae (1037) were utilized. After inoculation, the insects were left at 25±0.5°C. In order to make observations, samples from both castes were removed at 0, 6, 12, 24, 48, 72, 96, 120, 144, and 168h after inoculation. The external development of M. anisopliae and B. bassiana conidia on the termite C. cumulans showed that M. anisopliae and B. bassiana conidial germination occurred on several regions of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Microbial control; Insect pathogen; Termite.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2004000100010

Screening of Metarhizium Anisopliae (Metsch.) sorok. strains against the sugarcane root spittlebug Mahanarva Fimbriolata (Stål) (Hemiptera: Cercopidae) in laboratory
Provedor de dados: Neotropical Entomology
Autores: Loureiro,Elisângela de S.; Batista Filho,Antonio; Almeida,José E.M.; Pessoa,Luis G.A.
The accumulated mulch on the soil resultant from the mechanized cutting of sugarcane promotes favorable temperature and moist to Mahanarva fimbriolata (Stål), a pest that causes serious problems due to its widespread occurrence and high populations. Seventy nine isolates of Metarhizium anisopliae (Metsch.) Sorok. from different hosts and regions were tested for their pathogenicity. Ten spitlebugs were put in petri dishes (9 cm of diameter) containing sugarcane leaves sprayed with 1 ml of a suspension containing 5 x 10(7) conidia/ml. Five replicates were used per treatment. The petri dishes were kept at 25 ± 1ºC, 70 ± 10% RH and 12h photophase. Insects were monitored daily to calculate total cumulative mortality, as well as corrected and confirmed mortality...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Enthomopathogenic fungi; Biological control; Microbial control.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000500011

ACTIVITY OF SOME BRAZILIAN ISOLATES OF ENTOMOPATHOGENIC FUNGI AGAINST THE POULTRY RED MITE DERMANYSSUS GALLINAE DE GEER (ACARI: DERMANYSSIDAE)
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Kasburg,CR; Alves,LFA; Oliveira,DGP; Rohde,C.
ABSTRACT Poultry red mite Dermanyssus gallinae is a cosmopolitan and hematophagous species commonly found in layer houses around the world. Poultry mite infestations may cause anemia, stress, low body weight and egg production, and mortality. Mite control is typically based on chemical products, but they are not effective and leave residues in eggs; therefore, alternative control methods, such as entomopathogenic fungi, need to researched. This study aimed at evaluating, in the laboratory, the activity of Brazilian isolates of entomopathogenic fungi against D. gallinae. The mites were collected from a commercial layer house and were sprayed with conidial suspensions (1 × 108 conidia/mL) of five isolates of Beauveria bassiana and Metarhizium anisopliae. All...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematophagous mites; Microbial control; Ectoparasites; Laying hens.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000300457

Comparative bio-efficacy of nuclear polyhedrosis virus (NPV) and Spinosad against American bollwormm, Helicoverpa armigera (Hubner)
Provedor de dados: Revista Brasileira de Entomologia
Autores: Nawaz,Ahmad; Ali,Habib; Sufyan,Muhammad; Gogi,Muhammad Dildar; Arif,Muhammad Jalal; Ranjha,Mazhar Hussain; Arshid,Muhammad; Waseem,Muhammad; Mustafa,Tariq; Qasim,Muhammad; Rizwan,Muhammad; Zaynab,Madiha; Khan,Khalid Ali; Ghramh,Hamed A.
ABSTRACT American bollworm (ABW), Helicoverpa armigera (Hubner), is considered as a major pest of cotton, Gossypium hirsutum, all over the globe. Due to its destructive feeding nature and continuous consumption of the same chemicals, it devolved resistant against many insecticides. Therefore, a combined application of bio- and synthetic-pesticide need to evaluate against this pest. The entomopathogenic viruses like nuclear polyhedrosis virus (NPV), a member of baculoviruses, can be the potential candidates for better control against ABW. The present study was conducted to assess the comparative efficacy of NPV and Spinosad 240SC (with the concentration of 250 mL · ha-1) against ABW in the controlled environment. The ABW was treated with different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: American bollworm; Bio-pesticide; Insecticide; Microbial control; NPV.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262019000400277

Occurence of thrips on Niagara table grape and its control with the insecticides thiacloprid and methiocarb associated with Metarhizium anisopliae
Provedor de dados: Revista Brasileira de Fruticultura
Autores: LOPES,ROGÉRIO BIAGGIONI; TAMAI,MARCO ANTONIO; ALVES,SÉRGIO BATISTA; SILVEIRA NETO,SINVAL; SALVO,SÉRGIO DE.
Thrips are reported as important pests on table grapes in United States and several countries of Europe. Damage caused by thrips, particulary Frankliniella occidentalis, was observed on niagara table grape crop in Limeira-SP, Brazil. During the blooming period, high thrips densities were observed feeding on pollen and small berries. The symptoms left were more visible after the development of the berries and were characterized by dark scars and suberized surface on berries, sometimes causing the berry to crack, and the seed to prolapse. The effect of insecticides thiacloprid or methiocarb, associated or not with the entomopathogenic fungus Metarhizium anisopliae were evaluated during the blooming period. For evaluation of thrips damage on fruits, the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Chemical control; Fungi; Microbial control; Thrips.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452002000100060

Selection of Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok. for the control of the mite Mononychellus tanajoa (Bondar)
Provedor de dados: Scientia Agricola
Autores: Barreto,Rodrigo Soares; Marques,Edmilson Jacinto; Gondim Jr.,Manoel Guedes Corrêa; Oliveira,José Vargas de.
The green mite, Mononychellus tanajoa (Bondar) (Acari: Tetranychidae), is considered to be one of the key pests in cassava, Manihot esculenta Crants, leading to considerable field losses. In this study, ten Beauveria bassiana (Bals.) Vuill. and ten Metarhizium anisopliae (Metsch.) Sorok. isolates were evaluated with regard to their potential as biological control agents against adult M. tanajoa females. The total mortality percentage of M. tanajoa caused by B. bassiana ranged from 13.0 to 97.0%, with confirmed mortality rates extending from 9.0 to 91.0% and LT50 varying from 4.2 to 17.0 days. The M. anisopliae isolates showed total mortality percentages ranging from 12.0 to 45.0% with confirmed mortality rates extending from 8.0 to 45.0%, and LT50 varying...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Entomopathogenic fungi; Cassava green mite; Microbial control.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000600015

Selection of Metarhizium anisopliae (Metsch.) and Beauveria bassiana (Bals.) isolates to control Alabama argillacea (Huebner) caterpillars
Provedor de dados: Scientia Agricola
Autores: César Filho,Everardo; Marques,Edmilson Jacinto; Barros,Reginaldo.
The cotton leafworm, Alabama argillacea (Huebner, 1818) (Lepidoptera: Noctuidae), is considered to be one of the key pests in herbaceous cotton (Gossypium hirsutum L. r. latifolium Hutch) cropping, with constant occurrence in all cotton-growing states of Brazil. In this study Metarhizium anisopliae and Beauveria bassiana isolates were screened and evaluated for pathogenicity against Alabama argillaceae. Initially, a screening of ten isolates of each fungus in a concentracion of 10(8) conidia mL-1, was carried out on 3rd instar larvae of A. argillacea. Further studies were conducted to determine the pathogenicity and virulence of six and seven isolates of M. anisopliae and B. bassiana, respectively, against 3rd instar larvae of A. argillacea and using the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Entomopathogenic fungus; Cotton leafworm; Microbial control.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000300008

Compatibility between the entomopathogenic fungus Beauveria bassiana and insecticides used in coffee plantations
Provedor de dados: Scientia Agricola
Autores: Oliveira,Carolina Natali de; Neves,Pedro Manuel Oliveira Janeiro; Kawazoe,Lídio Sueki.
Microbial control in integrated pest management (IPM) programs of coffee plantations is an important factor for the reduction of pest population densities. The use of selective pesticides can be associated with entomopathogens, increasing the efficiency of the control and reducing the use of required insecticides. The in vitro fungitoxic effect of insecticide formulations of Thiamethoxam, Cyfluthrin, Deltamethrin, Alpha-Cypermethrin, Triazophos, Chlorpyrifos, Fenpropathrin and Endosulfan and Beauveria bassiana (CG 425 strain) was evaluated at three concentrations (FR = average field recommendation; 0.5 ´ FR and 2 ´ FR). Effects of these products on conidia germination, vegetative growth and sporulation were compared. Only five insecticides, at the FR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbial control; Toxic effect; Pesticides.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400009

External development of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in the subterranean termite Heterotermes tenuis
Provedor de dados: Scientia Agricola
Autores: Moino Jr.,Alcides; Alves,Sérgio Batista; Lopes,Rogério Biaggioni; Neves,Pedro Manuel Oliveira Janeiro; Pereira,Roberto Manoel; Vieira,Solange Aparecida.
The subterranean termite Heterotermes tenuis is one of the main pests of sugarcane and eucalyptus in Brazil, and the use of entomopathogenic fungi, alone or associated to chemicals, is an efficient and environmentally favorable method for its control. Studies related to the fungal development on these insects are important due to the effect of insect behavior on entomopathogens. The objective of this work was to describe the external development of Beauveria bassiana and Metarhizium anisopliae on H. tenuis using Scanning Electron Microscopy (SEM), determining the duration of the different phases of fungal infection. Two fixation techniques for preparing SEM samples were also evaluated. Worker specimens of H. tenuis were inoculated with a 1 x 10(9) conidia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Isoptera; Microbial control; Scanning electron microscopy.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000200010

Estabilidade de uma formulação de Bacillus sphaericus armazenada sob diferentes temperaturas
Provedor de dados: Scientia Agricola
Autores: Alves,Luis Francisco Angeli; Alves,Sérgio Batista; Lopes,Rogério Biaggioni; Augusto,Nílson Toschi.
O objetivo deste trabalho foi avaliar a estabilidade de uma formulação granulada de Bacillus sphaericus à base de sabugo de milho, armazenada em condições ambientais e a 50°C, tendo como comparação, uma suspensão concentrada de esporos e cristais da bactéria. A análise periódica dos materiais armazenados revelaram o efeito protetor da formulação sobre o microrganismo, de forma que ao longo de 12 meses de armazenamento, em condições ambiente, a viabilidade dos esporos foi maior que na suspensão concentrada. Além disso, a atividade inseticida para larvas de Culex quinquefasciatus, avaliada nos criadouros artificiais foi comparável ao patógeno não formulado. Quando mantidos a 50°C, a ação protetora da formulação mostrou-se mais eficiente, sendo que ao final...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Culex quinquefasciatus; Entomopatógeno; Controle microbiano; Culex quinquefasciatus; Entomopathogen; Microbial control.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162001000100004

Compatibility of polymers to fungi Beauveria bassiana and Metarhizium anisopliae and their formulated products stability
Provedor de dados: Acta Scientiarum: Agronomy
Autores: Wenzel Rodrigues, Inajá Marchizeli; Batista Filho, Antonio; Giordano, Isabella Barichello; Denadae, Bárbara Evelin; Fernandes, João Batista; Forim, Moacir Rossi.
Stability during storage is a limiting factor in the development and use of bioinsecticides. This study aims to evaluate the compatibility of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana with components used in encapsulated formulations and to evaluate shelf-life in formulations with B. bassiana. Sodium alginate and maltodextrin, hemicellulose, and dimethyl sulfoxide solvents were evaluated. Compatibility was evaluated by mixing the components with PDA, and the parameters evaluated were vegetative growth, sporulation and viability. The formulations of fungi were prepared in various concentrations of sodium alginate and then added drop-wise to calcium chloride. To evaluate the stability, formulations were stored at 26.5, 4.0, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbial control; Formulations; Entomopathogens; Encapsulation; Shelf-life; Microbiology.
Ano: 2017
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/32903

Toxicity of Bacillus species to larvae of Lutzomyia longipalpis (L. &amp;amp; N.) (Diptera: Psychodidae: Phlebotominae)
Provedor de dados: Anais da Sociedade Entomológica do Brasil
Autores: Wermelinger,Eduardo D.; Zanuncio,José C.; Rangel,Elizabeth F.; Cecon,Paulo R.; Rabinovitch,Leon.
A study was conducted to compare the susceptibility of third instar larvae of Lutzomyia longipalpis (L. &amp;amp; N.) (Diptera: Psychodidae: Phlebotominae), the vector of the American visceral leishmaniasis to two strains of Bacillus thuringiensis serovar israelensis, one strain of Bacillus sphaericus (all pathogenic to Diptera Culicidae) and a strain of B. thuringiensis ser. morrisoni (pathogenic to larvae of Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae)). Larvae of L. longipalpis showed similar susceptibility to the two strains of B. thruringiensis ser. israelensis, while B. sphaericus and B. thuringiensis ser. morrisoni showed low and no larvicidal effect to this vector, respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Microbial control; Leishmaniasis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80592000000300025

Safety of Beauveria bassiana (Bals.) Vuill. to Gallus domesticus L.
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Haas-Costa,Jucelaine; Alves,Luis Francisco Angeli; Daros,Alaxsandra Aparecida.
Beauveria bassiana, an important insect pathogen was used to evaluate its effect on the feeding, behavior, histology of the digestory system and anatomy of Gallus domesticus. The fungus (strain Unioeste 4) was administrated orally treated with chicken food. Chicken feces were collected, and the birds observed for 28 days. Also, were evaluated the weight of the birds, as well as any sign of intoxication or pathological modification. Tissue samples were withdrawn to test lesions with the optic microscope. There was 100% of survival of the birds, and no behavior alteration or external lesion was found. The test group presented the highest weight gain (1383.9±54.4g). Viable conidia were observed in the feces until 24 h after feeding the fungus, indicating that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Microbial control; Non-target organisms; Birds.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200027

Winter prevalence of obligate aphid pathogen Pandora neoaphidis mycosis in the host Myzus persicae populations in southern China: modeling description and biocontrol implication
Provedor de dados: Brazilian Journal of Microbiology
Autores: Zhou,Xiang.
Pandora neoaphidis overwintering had been investigated by monitoring its prevalence in Myzus persicae populations in open fields. Cabbage plants in field plots were weekly taken after mycosis initiation, to count and examine the living and dead aphids infected by P. neoaphidis. Based on the field data, infection levels (I) varied with field temperature (T), relative humidity (RH) and aphid count (numbers of living aphids per plant, N) over days (D), fitting well to the modified logistic equation I=0.91/[1+exp(8.5+(2.0H T H RH-20.2NI0)D)] (r²=0.897), where H T indicated daily hours of low temperature (&lt;4°C), H RH daily hours of high air humidity (&amp;gt;90% RH) and I0 primary infection level. The model demonstrated the abiotic and biotic factors...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Entomopathogenic fungi; Aphid; Epizootic modeling; Entomophthorales; Microbial control.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100038

Effectiveness of Metarhizium anisopliae against immature stages of Anastrepha fraterculus fruitfly (Diptera : Tephritidae)
Provedor de dados: Brazilian Journal of Microbiology
Autores: Destéfano,Ricardo Henry Rodrigues; Bechara,Ivanira José; Messias,Claudio Luiz; Piedrabuena,Aquiles Eugênico.
The study evaluated the effectiveness of Metarhizium anisopliae var. anisopliae (Hyphomycetes : Moniliales) strain E9, isolated from the pasture spittlebug Deois flavopicta (Hemiptera : Cercopidae), against larvae, prepupae and pupae stage and emergent adults of Anastrepha fraterculus, the South American fruitfly. The bioassay was carried out simulating field conditions, on autoclaved (AS) and non-autoclaved (NAS) soil from typical citrus orchards in the State of São Paulo, Southeastern region of Brazil. Various concentrations of conidia were incorporated into the soil the mortality, calculated based on the percentage of adult emergence, was 86% for the highest conidia concentrations: 2.52 x 10(10) for AS and 2.52 x 10(10) for NAS. The lethal concentration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbial control; Metarhizium anisopliae; Anastrepha fraterculus; Fruitfly; Bioassay.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100018

Sporulation of Metarhizium anisopliae var. Acridum and Beauveria bassiana on Rhammatocerus schistocercoides under humid and dry conditions
Provedor de dados: Brazilian Journal of Microbiology
Autores: Magalhães,Bonifácio Peixoto; Goettel,Mark Stanislaw; Frazão,Heloisa da Silva.
The sporulation of the fungi Metarhizium anisopliae var. acridum and Beauveria bassiana in cadavers of the grasshopper Rhammatocerus schistocercoides was studied in dry and humid environments. Both fungi were equally virulent against R. schistocercoides. However, internally, M. anisopliae produced more conidia than B. bassiana at 53% and 75% relative humidity. Externally, there was no sporulation at 53% and 75% RH, and M. anisopliae produced more conidia than B. bassiana at 100% RH.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Entomopathogenic fungi; Internal sporulation; Grasshoppers; Microbial control.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300002

Mechanism of infection and colonization of Rhipicephalus sanguineus eggs by Mertarhizium anisopliae as revealed by scanning eletron microscopy and histopathology
Provedor de dados: Brazilian Journal of Microbiology
Autores: Garcia,Marcos Valerio; Monteiro,Antonio Carlos; Szabo,Matias Juan Pablo; Prette,Nancy; Bechara,Gervásio Henrique.
The present work aimed to verify the penetration pattern of the Metarhizium anisopliae fungus into Rhipicephalus sanguineus tick eggs, as well as the lesions occurred inside the egg. The fungus adherence and penetration were studied by scanning electron microscopy and the action of fungus in the internal tissues was evaluated through conventional histological sessions. For the observation of these events, experimental infections were performed in 11 groups of R. sanguineus eggs containing 25 mg each. The eggs were bathed during 3 minutes under manual shaking in a 10(8) conidia/mL suspension. The bath was performed only in the suspension vehicle for the control groups. The eggs were processed for histopathological analysis and scanning electron microscopy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Microbial control; Entomopathogenic fungus; Ticks.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400012

Total lipids and fatty acids of strains of Metarhizium anisopliae
Provedor de dados: Brazilian Journal of Microbiology
Autores: Pupin,Antonio Marcos; Messias,Claudio Luiz; Piedrabuena,Aquiles Eugênico; Roberts,Donald Wilson.
Two growth stages, conidia (C) and mycelium (M), and two media, minimal medium (MM) and complete medium (MC), were compared in 10 strains of M. anisopliae, and two strains of M. anisopliae var. majus were similar in percentages of total lipids. Tukey test for average of lipid content in conidia (C) and mycelia (M) cultured on minimal (MM) and complete (MC) media showed significant differences between means at the 5% level for mycelia and conidia, indicating variability in total lipid production and storage during growth. Strains 5 and 7, both variety majus, did not present sizable differences from variety anisopliae. For fatty acids, C18:1 and C18:2, oleic and linoleic, respectively, the differences were all highly significant (p= 1%) with the highest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipids; Fatty acids; Metarhizium anisopliae; Entomopathogenic fungi; Microbial control.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200012

Bioassay assessment of metarhizium anisopliae (metchnikoff) sorokin (deuteromycota: hyphomycetes) against Oncometopia facialis (signoret) (hemiptera: cicadellidae)
Provedor de dados: Brazilian Journal of Microbiology
Autores: Pria Júnior,Wolney Dalla; Lacava,Paulo Teixeira; Messias,Claudio Luiz; Azevedo,João Lúcio; Lacava,Pedro Magalhães.
Citrus Variegated Chlorosis (CVC) is an economically important, destructive disease in Brazil and is caused by Xylella fastidiosa and transmitted by sharpshooter insects. In this study, the efficacy of the fungus Metarhizium anisopliae in controlling the sharpshooter Oncometopia facialis was studied by bioassay conditions. In the bioassay, insects were sprayed with a suspension containing 5 X 10(7) conidia mL-1. Adults captured in the field were treated in groups of 10 in a total of 11 replications per treatment. Significant differences between the natural mortality and the mortality of insects treated with the fungus were observed 6 days after inoculations (P&lt;0.05). These significant differences increased until 10 days after treatment. The fungus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbial control; Entomopathogen; Citrus Variegated Chlorosis (CVC); Bioassay.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100027

Genetic variability of brazilian Alternaria spp. isolates as revealed by RAPD analysis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Tigano,Myrian Silvana; Aljanabi,Salah; Mello,Sueli Corrêa Marques de.
The genetic variability of 22 isolates of the fungi Alternaria alternata and A. cassiae, obtained from Senna obtusifolia, was studied by RAPD analysis. A total of 491 scorable bands were produced with the use of 28 primers. Cluster analysis based on similarities computed from RAPD markers showed two distinct genetic groups of isolates related to both species. RAPD analysis proved to be an efficient method for detecting genetic variability of A. cassiae and A. alternata isolates occurring in S. obtusifolia, and also for distinguishing Alternaria species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fungi; Senna obtusifolia; Bioherbicide; Microbial control.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200005

Influence of culture media in viability test of conidia of entomopathogenic fungi
Provedor de dados: Ciência Rural
Autores: Francisco,Edimara Aparecida; Mochi,Dinalva Alves; Correia,Antônia do Carmo Barcelos; Monteiro,Antonio Carlos.
This work aimed at investigatimg whether the culture medium used in viability tests affects the conidial germination of Lecanicillium lecanii, Beauveria bassiana and Paecilomyces fumosoroseus isolates. The tests were performed on microscope slides containing one of the culture media: agar-water (AW), minimal medium (MM), potato-dextrose agar (PDA), potato-dextrose-1% yeast extract agar (PDAY), Sabouraud-dextrose-yeast extract agar (SDAY), and complete medium (CM). Three areas per slide were delimited and 0.05ml of a 5.5 x 105 conidia ml-1 suspension was applied to each area. One bioassay was performed for each isolate. Germination was determined after 15 hours of incubation at 26±0.5°C. The culture media influenced the germination of the species studied,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beauveria bassiana; Lecanicillium lecanii; Paecilomyces fumosoroseus; Germination; Microbial control.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000400043