Consulta: subjectFacets:"Microbiological analysis"
Registros recuperados: 19
Data/hora: 05/06/2024 17:00:36

The dispersion of adhered marine bacteria by pyrophosphate and ultrasound prior to direct counting.
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Velji, M; Albright, L.
A technique has been investigated for dispersing adhered marine bacteria from various surfaces prior to direct counting. The bacterial cells were initially preserved and strengthened by use of 3.7% formaldehyde (v/v final concentration). Seawater samples were then treated with 0.001 M of tetrasodium pyrophosphate (final concentration), a sequestering and deflocculating agent. Surface sediment and kelp disc samples were treated with 0.01 M tetrasodium pyrophosphate (final concentration) after having been suspended in 0.44 M sodium chloride solution. These samples were incubated for 15-30 min. Subsequently, the samples were treated with ultrasound (100 W) for 30 to 45 s. This technique resulted in an even dispersion of most bacterial cells in the samples....
Tipo: Text
Palavras-chave: Bacteria; Microbiological analysis; Flocculation; Ultrasonics; Bacterial counters.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-968.pdf

Methods for microbial biomass, community structure and metabolic activities on surfaces.
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: White, D.
Microbes in nature are often concentrated on surfaces where they exist in microcolonies of mixed composition. The assay of these mixed microbial consortia by utilizing biochemical components eliminates the problems associated with cultural selectivity of viable counts or the requirement for quantitative release from surfaces required in some microscopic techniques. The assay of cellular components restricted to subsets of the microbial community gives insights into the community structure of the biofilm whereas assay of cellular components common to all cells can be used for biomass estimations. Formation of endogenous storage materials can be utilized as measures of nutritional status and rates of formation or turnover of specific "signatures" from...
Tipo: Text
Palavras-chave: Bacteria; Fouling; Bioassays; Metabolism; Surfaces; Bacteria collecting devices; Culture media; Microbiological analysis.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-963.pdf

Sampling for a bacteriological study of aquatic enrironments
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Troussellier, M; Baleux, B; Andre, P.
Using water samples from several observation levels of a brackish lagoon, statistical solutions are offered for the sampling optimisation of bacteria viable counts. The variability between plate count is studied by the mean of the Taylor's power law. The sampling strategy optimisation, including several sources of variability, is realized by hierarchical analysis of variance and the estimation of the sampling cost of each source. This optimisation procedure results in a great increase of the relative efficiency of the sampling design and allow to obtain significant differences between the bacterial concentrations. The effect of the reduction of the total sampling effort on the precision of these average concentrations pointed out the scientific and...
Tipo: Text
Palavras-chave: Bacteria; Abundance; Variance analysis; Microbiological analysis; Lagoons; Brackishwater environment; Bacterial counters; Biological sampling.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1984/acte-941.pdf

Isolement et identification de vibrions halophiles d'une station experimentale de mariculture
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Bruni, V; Crisafi, E; Maugeri, T; Zaccone, R.
110 Vibrio strains were isolated, at various times of the year, from samples of sea-water supply, breeding-tank water and discarded fish used as food at the breeding stations. From the results obtained by the biochemical and cultural tests, it was possible to identify most of the isolated strains as Vibrio anguillarum . Strains with intermediate characteristics, above-all positive saccharose, proved to be numerous. The authors discuss the occurrence, in particular environments such as those being studied, of atypical or intermediate vibrio strains which are difficult to classify.
Tipo: Text
Palavras-chave: Vibrio; Microbiological culture; Bacteria; Food; Minced products; Fish ponds; Sea water; Identification; Marine aquaculture; Bacteria collecting devices; Microbiological analysis.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-997.pdf

Effects of environmental conditions on the sessile existence of an estuarine sediment bacterium.
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Geesey, G; Salas, S; Mittelman, M.
A submerged glass coverslip technique was developed to determine dissolved organic nutrient uptake by adherent cells of a sediment isolate of Enterobacter cloacae . Cells which colonized the coverslips in swirling culture flasks during anaerobic growth remained firmly attached during manipulations employed to determine uptake of radiolabeled glucose by the adherent population. The attached cells were capable of a more rapid rate of glucose uptake than free cells. Lineweaver-Burk plots demonstrated different glucose uptake kinetics for the 2 cell populations. The data suggest that physiological changes occur in cells soon after they become attached to surfaces. These changes appear to enhance the metabolic activity of the adherent population.
Tipo: Text
Palavras-chave: Enterobacter cloacae; Adhesion; Glucose; Environmental effects; Bacteria; Sediments; Estuaries; Sessile species; Culture media; Metabolism; Dissolved oxygen; Microbiological analysis.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-967.pdf

Etude experimentale de la colonisation de surfaces par les bacteries, en eau de mer circulante
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Fera, P; Prieur, D.
Before the building of an (OTEC) power plant, and experimental station was built near the entrance of the Bay of Brest in order to do preliminary studies. Using these facilities, the bacterial colonization of three substrata (stainless steel UB6, aluminium AG4, polycarbonate filters) was studied in an open seawater system, with a speed of 0,7 m s super(-1). The study was performed between May 1983 and May 1984, including seven experiments of 15 days each. Bacteria colonizing the metallic surfaces were counted by scanning electron microscopy, and bacteria colonizing the polycarbonate filters counted by epifluorescence microscopy. On the three substrata, bacterial colonization occurred in three successive stages: initial colonization, sometimes unsteady...
Tipo: Text
Palavras-chave: Fouling; OTEC plants; Bacteria; Colonization; Adhesion; Microbiological analysis; Surface films.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-964.pdf

Seasonal variation of microbial population from sediments of Vellar Estuary, South India.
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lakshmanaperumalsamy, P; Chandramohan, D; Natarajan, R.
A seasonal study on the occurrence and distribution of total aerobic microbial population (TMP), total aerobic heterotrophic bacteria (THB), actinomycetes and fungi in the sediments of Vellar Estuary, South India (11 degree 29'N 79 degree 46'E) was carried out during Jan. to Dec. 1975. Sediment samples were collected at monthly intervals, from 4 stations and analysed microbiologically. In general, bacterial population was found to be dominant followed by actinomycetes and fungi. The max peak for TMP, THB and fungi were recorded in Nov. in all stations. Monsoon season supported TMP, THB and fungi to a high level and it was summer for actinomycetes. Statistical analysis (Kendall's tau) showed a significantly positive correlation among TMP, THB, actinomycetes...
Tipo: Text
Palavras-chave: Actinomycetes; Population number; Statistical analysis; Seasonal variations; Fungi; Bacteria; Sediment analysis; Microbiological analysis.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-943.pdf

Estudios de enterobacterias en las inmediaciones de Punta Fort William, Isla Greenwich
Provedor de dados: Ocean Docs: e-repository of  publications
Autores: Helguero, C.; Valencia T., M.
Se presenta un estudio microbiológico de las aguas de Ensenada Guayaquil y Bahía Chile, con el propósito de determinar presencia de enterobacterias en las mismas; se detectó la existencia de tres grupos: Escherichiae, Yersinieae y Klebsielleae. Se estima que eventualmente dicha presencia es ocasionada por la presencia de la actividad humana en el área y por el aporte de los excrementos de las aves transportadas en aguas provenientes de deshielos en el verano.
Tipo: Journal Contribution
Palavras-chave: Microbiological analysis; Microorganisms; Polar waters; Microbiological analysis; Microorganisms; Http://aims.fao.org/aos/agrovoc/c_25302; Http://aims.fao.org/aos/agrovoc/c_4807.
Ano: 1993
URL: http://hdl.handle.net/1834/3808

Estudiosd bacteriológicos en la Ciénaga Grande de Santa Marta, Colombia
Provedor de dados: Ocean Docs: e-repository of  publications
Autores: Jeske, R.
Mientras predominan las aguas salobres en la Ciénaga Grande se destacan las formas bacterianas halófilas. Durante la época de lluvias (invierno, disminuye la salinidad, sin embargo las formas halófilas aumentan en número. La reproducción de bacterias de agua dulce se inicia sólo cuando la salinidad llega bajo un valor de un 10°/o/o. Estas alcanzan su valor máximo temporáneo en salinidades de un 3°/o/o. Al mismo tiempo disminuyen las formas halófilas. Observaciones sobre contaminación fecal se realizaron en las aguas de la Ciénaga. Como indicador se utilizó Escherichia coli. En las inmediaciones de los pueblos de pescadores es posible detectar cierta contaminación. En cambio, en el resto de la Ciénaga, en contados casos se observa E. coli. La presencia de...
Tipo: Journal Contribution
Palavras-chave: Coastal lagoons; Estuaries; Microbiological analysis; Microbial contamination; Estuaries; Microbial contamination; Coastal lagoons; Microbiological analysis; Http://aims.fao.org/aos/agrovoc/c_2673; Http://aims.fao.org/aos/agrovoc/c_37547; Http://aims.fao.org/aos/agrovoc/c_1697; Http://aims.fao.org/aos/agrovoc/c_25302.
Ano: 1976
URL: http://hdl.handle.net/1834/3322

Cambios en la composición de pequeños pescados pelágicos salados en salmuera durante su producción y almacenamiento
Provedor de dados: Ocean Docs: e-repository of  publications
Autores: Bostock, T.; Camba, N.
Los cambios físicos, químicos y organolépticos en macarela (Scomber japonicus) y sardina (Sardinops sagax, Etrumeus teres) saladas durante el procesamiento y subsecuente almacenamiento en salmuera son descritos. Es evaluada además la calidad del producto desde el punto de vista microbiológico, así como también los efectos que se observan en el producto final cuando el artesano ecuatoriano usa materia prima de baja calidad. Los principales cambios que ocurren (enrojecimiento de la carne, desarrollo de olores a frutas, el aumento de las bases volátiles totales y de proteínas solubles y posiblemente histamina parecen estar relacionadas más con la autólisis que con el desarrollo microbiológico). La maduración o ”deterioración” (en términos del paladar...
Tipo: Journal Contribution
Palavras-chave: Processing fishery products; Storage effects; Chemical composition; Physical properties; Organoleptic properties; Microbiological analysis; Cured products; Chemical composition; Physical properties; Organoleptic properties; Microbiological analysis; Http://aims.fao.org/aos/agrovoc/c_1794; Http://aims.fao.org/aos/agrovoc/c_13328; Http://aims.fao.org/aos/agrovoc/c_5399; Http://aims.fao.org/aos/agrovoc/c_25302.
Ano: 1986
URL: http://hdl.handle.net/1834/3153

Estudio comparativo de la calidad del agua en el área marino costera de Bahía Academia, Caleta Aeolian y Puerto Villamil - Islas Galápagos -  junio-julio 2007
Provedor de dados: Ocean Docs: e-repository of  publications
Autores: Palacios, C.; Burgos, L.
Se analiza los datos tomados en el área marino costera de tres importantes islas de Galápagos como son: Bahía Academia-Santa Cruz, Caleta Aeolian-Baltra y Puerto Villamil-Isabela, durante los meses de junio y julio del 2007, considerando la distribución superficial de los parámetros físicos, químicos en cada una de las áreas. Se efectúa el análisis comparativo de las concentraciones promedios y la calidad microbiológica con los límites permisibles establecidos en la Legislación Secundaria Ambiental, concluyendo en general, que la calidad del agua está acorde con la vida marina, pero que a pesar de no presentar mayores problemas de contaminación, existen área muy puntuales que ameritan mayor cuidado y seguimiento para preservar y conservar estas islas que...
Tipo: Journal Contribution
Palavras-chave: Water quality; Microbiological analysis; Physical properties; Data processing; Water quality; Microbiological analysis; Physical properties; Data processing; Http://aims.fao.org/aos/agrovoc/c_16061; Http://aims.fao.org/aos/agrovoc/c_25302; Http://aims.fao.org/aos/agrovoc/c_13328; Http://aims.fao.org/aos/agrovoc/c_10289.
Ano: 2009
URL: http://hdl.handle.net/1834/3369

Effet de l’addition du thym, du laurier et du romarin sur la conservation de l’anguille fumée
Provedor de dados: Ocean Docs: e-repository of  publications
Autores: Zerai, T.; Mestiri, F.; Romdhane, M.S.; Mejri, S.
L’effet  de l’addition de trois types d’additifs naturels: le thym (Thymus vulgaris), le laurier (Laurus nobilis) et le romarin (Rosmarinus officinalis) sur l’amélioration de la conservation de l’anguille fumée a été étudié. Une comparaison des caractéristiques microbiologiques, physicochimiques et organoleptiques du produit, par rapport au lot témoin, fumé sans additifs a été réalisée durant 5 semaines à 4 °C. Nous avons trouvé que l’addition du thym, a permit  une réduction de la flore aérobie mésophyte totale (FAMT), a ralenti  la formation de l’azote basique volatil total (ABVT), et a permit  une réduction  de l’oxydation de la matière grasse. Le lot ainsi traité, a été très apprécié par les dégustateurs, et le thym a eu un effet améliorateur sur le...
Tipo: Journal Contribution
Palavras-chave: Chemical analysis; Cured products; Microbiological analysis; Processing fishery products; Quality; Storage life; Taste tests; Anguilla anguilla; Laurus nobilis; Rosmarinus officinalis; Http://aims.fao.org/aos/agrovoc/c_41152; Http://aims.fao.org/aos/agrovoc/c_4227; Http://aims.fao.org/aos/agrovoc/c_6660.
Ano: 2006
URL: http://hdl.handle.net/1834/4278

Analyses microbiologiques et chimiques des produits de la pêche et de l'eau
Provedor de dados: Ocean Docs: e-repository of  publications
Autores: Diouf, Sidiya.
Ce rapport porte principalement sur la microbiologie, la chaîne biochimie des aliments et des eaux au terme d'un stage d'un mois effectué au Laboratoire de Sécurité Alimentaire et d'Hygiène de l'Environnement (LSAHE) de l'Institut Pasteur de Dakar. Nous avons donné quelques notions générales sur: (1) les différents germes recherchés dans: - le poisson (frais ou transformé); germes capables d'altérer la qualité marchande du poisson (la flore totale à 30 ° C); - germes potentiellement pathogènes (ASR, Salmonellea, Staphylococcus); - germes témoins de contamination fécale (coliformes fécaux);  - germes indicateurs technologiques (coliformes totaux entérobactéries). (2) l'eau et la glcae: - germes capables d'altérer la qualité marchande de l'eau (la flore...
Tipo: Report
Palavras-chave: Microbiological analysis; Fish; Water analysis; Ice; Hygiene; Quality control; Fish; Ice; Microbiological analysis; Water analysis; Hygiene; Http://aims.fao.org/aos/agrovoc/c_15903; Http://aims.fao.org/aos/agrovoc/c_3782; Http://aims.fao.org/aos/agrovoc/c_25302; Http://aims.fao.org/aos/agrovoc/c_16064; Http://aims.fao.org/aos/agrovoc/c_3739.
Ano: 2001
URL: http://hdl.handle.net/1834/4074

Etude de la résistance aux antibiotiques des bactéries isolées de "mytilus galloprovincialis.
Provedor de dados: Ocean Docs: e-repository of  publications
Autores: El Bour, M.; Bouamama, K.; El Abed, O.
L’étude des résistances vis-à-vis de différentes substances antibiotiques pour des espèces bactériennes (aérobies et anaérobies) isolées chez Mytilus galloprovincialis a été réalisée par la méthode standard de l’antibiogramme. Ainsi, 50 souches bactériennes aérobies et 20 souches anaérobies appartenant à plus de 25 espèces différentes ont été isolées et testées. Les résultats obtenus montrent des profils à plusieurs résistances vis-à-vis des différentes familles  d’antibiotiques testés. Les résultats obtenus révèlent des profils à 12 résistances différentes notamment chez l’espèce anaérobie (Propioni acnes). Aussi, chez l’espèce aérobie pré dominante ( Aeromonas hydrophila) nous avons mis en évidence au moins 6 antibiotypes différents. Ces résultats...
Tipo: Journal Contribution
Palavras-chave: Antibiotics; Bacterial diseases; Control resistance; Ecology; Eutrophication; Indicator species; Microbiological analysis; Bacteria; Mytilus galloprovincialis; Http://aims.fao.org/aos/agrovoc/c_765; Http://aims.fao.org/aos/agrovoc/c_31155.
Ano: 2006
URL: http://hdl.handle.net/1834/4266

Variabilidad hidroclimática en el Golfo de Batabanó, Cuba
Provedor de dados: Ocean Docs: e-repository of  publications
Autores: Piñeiro, R.; Cobas, S.; Fernández, L.
An study of hydroclimate variability in the region of the gulf of Batabanó analyzing the water and air monthly temperatures series from 1982 to 2008 was carried out. The correlation between both variables were r = 0,87 and was obtained an equation that allow to reconstruct the water temperature series from 2003 until 2008.The water temperature average was 27,60 ºC, with standard deviations of 1,496. In the anomalies fluctuations are observed warm and cold periods. The maximum positive anomalies appear in 1997-1998 and 2002-2003. In the transformed series of the annual water temperature average, a tendency to the temperature increase at the end of the 1990´s is obtained, until the 2008. The transformed series of the air temperature average had warming and...
Tipo: Preprint
Palavras-chave: Rivers; Microbiological analysis; Rivers; Microbiological analysis; Http://aims.fao.org/aos/agrovoc/c_6617; Http://aims.fao.org/aos/agrovoc/c_25302.
Ano: 2010
URL: http://hdl.handle.net/1834/3526

Estudios químicos y bacteriológicos del río Baradero (Argentina): calidad sanitaria del agua y aptitud de los peces para consumo humano
Provedor de dados: Ocean Docs: e-repository of  publications
Autores: Costagliola, M.; Seigneur, G.N.; Jurquiza, V.
As a result of an agreement signed by the Municipal Government of Baradero Buenos Aires Province, Argentina and the INIDEP, the sanitary quality of the Baradero River waters was studied and fish safety assessed. Results of five field surveys carried out between 1993 and 1997 are studied. Organochlorine and organophosphorate pesticide residues, as well as traces of heavy metals were analyzed in 49 fish specimens and aerobic heterotrophic bacteria (AHB), total coliform bacteria (TC) and fecal coliform bacteria (FC) were counted out in 7 sampling stations placed on the coast and the riverbed. Alpha Hexachlorohexane, gamma Hexachlorohexane, beta Hexachlorohexane, Aldrin, Heptachlor epoxide, pp'DDE and Dieldrin were detected in fish tissues. The values of AHB...
Tipo: Journal Contribution
Palavras-chave: Freshwater pollution; Pollution surveys; Chemical analysis; Water analysis; Microbiological analysis; Pesticides; Public health; Pathogenic bacteria; Pesticides; Public health; Water analysis; Microbiological analysis; Pathogenic bacteria; Http://aims.fao.org/aos/agrovoc/c_5739; Http://aims.fao.org/aos/agrovoc/c_6349; Http://aims.fao.org/aos/agrovoc/c_16064; Http://aims.fao.org/aos/agrovoc/c_25302; Http://aims.fao.org/aos/agrovoc/c_34317.
Ano: 2003
URL: http://hdl.handle.net/1834/2417

Selected bacterial strains protect &lt;i&gt;Artemia&lt;/i&gt; spp. from the pathogenic effects of &lt;i&gt;Vibrio proteolyticus&lt;/i&gt; CW8T2
Provedor de dados: Open Marine Archive
Autores: Verschuere, L.; Heang, H.; Criel, G.R.J.; Sorgeloos, P.; Verstraete, W.
In this study &lt;i&gt;Vibrio proteolyticus&lt;/i&gt; CW8T2 has been identified as a virulent pathogen for &lt;i&gt;Artemia&lt;/i&gt; spp. Its infection route has been visualized with transmission electron microscopy. The pathogen affected microvilli and gut epithelial cells, disrupted epithelial cell junctions, and reached the body cavity, where it devastated cells and tissues. In vivo antagonism tests showed that pre-emptive colonization of the culture water with nine selected bacterial strains protected &lt;i&gt;Artemia&lt;/i&gt; juveniles against the pathogenic effects. Two categories of the selected strains could be distinguished: (i) strains providing total protection, as no mortality occurred 2 days after the experimental infection with &lt;i&gt;V....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquatic animals; Bacteria; Biological control; Brine shrimp culture; Disease control; Infectious diseases; Microbiological analysis; Pathogenic bacteria; Artemia; Vibrio proteolyticus.
Ano: 2000
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=45

Enterobacteria identification and detection of diarrheagenic Escherichia coli in a Port Complex
Provedor de dados: Brazilian Journal of Microbiology
Autores: Costa,Clarissa Frota Macatrão; Monteiro Neto,Valério; Santos,Bruno Rafael de Carvalho; Costa,Bruno Rafael Rabelo; Azevedo,Alexandre; Serra,Josilene Lima; Mendes,Hermínio Benítez Rabello; Nascimento,Adenilde Ribeiro; Mendes,Mariana Bonfim Pinto; Kuppinger,Oliver.
The Port Complex of Maranhão (PCM) is the second largest port complex in Brazil, receiving ships with large volumes of ballast water. To evaluate the microbiological quality of its waters, physicochemical parameters (pH and salinity), the number of coliforms (thermotolerants and totals), and the presence of enterobacterias and diarrheagenic Escherichia coli strains were analyzed. In order to identify the presence of E. coli virulence genes target regions of the stx, elt, est, aggR, CVD432, ipaH and eae nucleotide sequences were studied. The presence of totals and thermotolerants coliforms were positive. Analyzing the salinity parameter, a significant increase in total coliforms was observed during the rainy season. We identified the species Escherichia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbiological analysis; Coliforms; Enterobacterias; Ballast water; Escherichia coli.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300026

Optimization of the osmotic dehydration of ginger
Provedor de dados: Ciência Rural
Autores: Siqueira,Leonardo Pereira de; Sarubbo,Leonie Asfora; Shinohara,Neide Kazue Sakugawa; Valencia,Marcela Sarmento; Cortez,Neila Mello Santos; Andrade,Samara Alvachian Cardoso.
ABSTRACT:  The present research aimed to optimize the process of osmotic dehydration (OD) of ginger with hypertonic sucrose solution employing response surface methodology. A 23 experimental planning was carried out and 17 experimental assays were performed based on three independent variables (temperature, concentration of the osmotic solution and immersion time) and three dependent variables (moisture loss (ML), solids gain (SG) and dehydration efficiency index (DEI)). The selected assay conditions exhibited the preferred DEI value (the highest ML and lower SG), which were considered in the optimization. Assay 16 demonstrated to be the most favorable conditions for the osmotic dehydration of ginger (DEI =1.61) at 40 40 °C, 50 °Brix and 90 minutes of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zingiber officinale Roscoe; Water loss; Microbiological analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001000754