Consulta: subjectFacets:"Microinjection"
Registros recuperados: 6
Data/hora: 05/06/2024 17:58:37

Optimization of transgenesis conditions for the generation of CXCL2-luciferase reporter mice line
Provedor de dados: 69
Autores: Crispo,Martina; Schlapp,Geraldine; Cárdenas-Rodríguez,Magdalena; González-Maciel,Dolores; Rumbo,Martín.
Background: Transgenesis by microinjection has been widely used for the generation of different mouse models. Different variables of the procedure may critically affect the efficiency of the process. A DNA construction that carries the CXCL2 promoter gene and firefly luciferase has been used to optimize aspects of the procedure. Three different concentrations (0.5, 1.0 and 4.0 ng/µl) of the DNA construction to microinject a total of 1981 zygotes has been tested. Intact/injected embryos, pregnancy and birth rate, survival of pups 7 days after birth, number of transgenic pups and overall transgenic efficiency was registered and analyzed by Z test of proportions for each group. Results: A total of seven transgenic founders were detected for the three DNA...
Tipo: Journal article
Palavras-chave: DNA concentration; Microinjection; Reporter systems.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600014

Improving the production of transgenic fish germlines: in vivo evaluation of mosaicism in zebrafish (Danio rerio) using a green fluorescent protein (GFP) and growth hormone cDNA transgene co-injection strategy
Provedor de dados: 74
Autores: Figueiredo,Márcio de Azevedo; Lanes,Carlos Frederico Ceccon; Almeida,Daniela Volcan; Marins,Luis Fernando.
In fish, microinjection is the method most frequently used for gene transfer. However, due to delayed transgene integration this technique almost invariably produces mosaic individuals and if the gene is not integrated into germ cells its transmission to descendants is difficult or impossible. We evaluated the degree of in vivo mosaicism using a strategy where a reporter transgene is co-injected with a transgene of interest so that potential germline founders can be easily identified. Transgenic zebrafish (Danio rerio) were produced using two transgenes, both comprised of the carp beta-actin promoter driving the expression of either the green fluorescent protein (GFP) reporter gene or the growth hormone cDNA from the marine silverside fish Odonthestes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transgenesis; Genetically modified fish; Microinjection; Growth hormone cDNA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100008

Mise au point de méthodes de manipulation embryonnaire de mollusques bivalves. Application en génétique et pathologie infectieuse
Provedor de dados: 5
Autores: Cadoret, Jean-paul.
Après vingt ans de programmes pivilégiant les méthodes d'élevage, les recherches concernant l'aquaculture, évoluent maintenant vers les domaines de la pathologie infectieuse et la génétique. La maîtrise des productions larvaires a en effet favorisé l'étude de la reproduction et de l'embryologie, et permet désormais d'aborder des travaux expérimentaux sur les différents stades embryonnaires. La polyploïdisation d'oeufs de moules ou d'huîtres a d'abord été envisagée par l'application d'un procédé d'électrofusion. Des expérimentations à grande échelle ont permis l'obtention d'embryons triploïdes (55% chez les huîtres et 36% chez les moules) ainsi que tétraploïdes (20% chez les huîtres et 26% chez les moules). La microinjection de spermatozoïdes a permis de...
Tipo: Text
Palavras-chave: Mollusques; Bivalves; Polyploïdie; Electrofusion; Microinjection; Fécondation assistée; Rickettsies; Embryoculture; Trasfert de gène; B-galactosidase; Résistance; Pathologie; Génétique.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/00440/55127/56585.pdf

Insecticidal activity of venomous saliva from Rhynocoris fuscipes (Reduviidae) against Spodoptera litura and Helicoverpa armigera by microinjection and oral administration
Provedor de dados: 80
Autores: Sahayaraj,K; Vinothkanna,A.
Rhynocoris fuscipes is a potential predator of many economically important pests in India. In the present study, its venomous saliva (VS) was collected by milking and diluted with HPLC grade water to different concentrations (200, 400, 600, 800 and 1000 ppm). Microinjection of Rhynocoris fuscipes VS was more toxic than its oral administration in Helicoverpa armigera (cotton bollworm) and Spodoptera litura (tobacco cutworm). Thus, R. fuscipes VS was found to be toxic to third instar S. litura and H. armigera with respective LD50s of 846.35 and 861.60 ppm/larva at 96 hours after microinjection. The current results showed that VS of Rhynocoris fuscipes caused mortality of H. armigera and S. litura. Active peptides from VS may be isolated, identified and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salivary venom; Microinjection; Oral administration; Mortality; Rhynocoris fuscipes; Biological control.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400016

Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP
Provedor de dados: 52
Autores: Finokiet,Manuela; Goni,Beatriz; Loreto,Élgion Lúcio Silva.
Studies were carried out on the use of piggyBac transposable element as vector and the green fluorescent protein (EGFP) from the jellyfish, Aquorea victoria, as a genetic marker for the transformation of Drosophila willistoni. Preblastoderm embryos of D. willistoni white mutant were microinjected with a plasmid containing the EGFP marker and the piggyBac ITRs, together with a helper plasmid containing the piggyBac transposase placed under the control of the D. melanogaster hsp70 promoter. G0 adults transformants were recovered at a frequency of approximately 67%. Expression of EGFP in larvae, pupae and adults was observed up to the third generation, suggesting that this transposon was not stable in D. willistoni. Transformed individuals displayed high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transposable elements; Insect transgenesis; Germ-line transformation; Fluorescent protein marker; Microinjection.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100013

Maternal aggression in Wistar rats: effect of 5-HT2A/2C receptor agonist and antagonist microinjected into the dorsal periaqueductal gray matter and medial septum
Provedor de dados: 56
Autores: Almeida,R.M.M. de; Giovenardi,M.; Silva,S.P. da; Oliveira,V.P. de; Stein,D.J.
The objective of the present study was to assess the role of the 5-HT2A/2C receptor at two specific brain sites, i.e., the dorsal periaqueductal gray matter (DPAG) and the medial septal (MS) area, in maternal aggressive behavior after the microinjection of either a 5-HT2A/2C receptor agonist or antagonist. Female Wistar rats were microinjected on the 7th postpartum day with the selective agonist alpha-methyl-5-hydroxytryptamine maleate (5-HT2A/2C) or the antagonist 5-HT2A/2C, ketanserin. The agonist was injected into the DPAG at 0.2 (N = 9), 0.5 (N = 10), and 1.0 µg/0.2 µl (N = 9), and the antagonist was injected at 1.0 µg/0.2 µl (N = 9). The agonist was injected into the medial septal area (MS) at 0.2 (N = 9), 0.5 (N = 7), and 1.0 µg/0.2 µl (N = 6) and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: 5-HT receptors; Ketanserin; Alpha-Methyl-5-hydroxytrypta- mine maleate; Microinjection; Serotonin.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400014