Consulta: subjectFacets:"Micronucleus"
Registros recuperados: 42
Data/hora: 04/06/2024 15:46:49

Assessment of the genetic risks of a metallic alloy used in medical implants
Provedor de dados: 74
Autores: Gomes,Cristiano C.; Moreira,Leonardo M.; Santos,Vanessa J.S.V.; Ramos,Alfeu S.; Lyon,Juliana P.; Soares,Cristina P.; Santos,Fabio V.
The use of artificial implants provides a palliative or permanent solution for individuals who have lost some bodily function through disease, an accident or natural wear. This functional loss can be compensated for by the use of medical devices produced from special biomaterials. Titanium alloy (Ti-6Al-4V) is a well-established primary metallic biomaterial for orthopedic implants, but the toxicity of the chemical components of this alloy has become an issue of concern. In this work, we used the MTT assay and micronucleus assay to examine the cytotoxicity and genotoxicity, respectively, of an extract obtained from this alloy. The MTT assay indicated that the mitochondrial activity and cell viability of CHO-K1 cells were unaffected by exposure to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomaterial; Cytotoxicity; Micronucleus; Mutagenicity; Ti-6Al-4V.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100020

Evaluation of the in vivo mutagenic potential of hydroalcoholic extracts of the northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) on peripheral blood cells of Swiss mice (Mus musculus Rodentia, Muridae)
Provedor de dados: 74
Autores: Freitas,Patrícia Scotini; Andrade,Sérgio Faloni de; Maistro,Edson Luis.
The northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) is very rich in anthocyanins, natural pigments which have strong antioxidant properties and potential health benefits, resulting in the worldwide use the blueberry as a medicinal plant. We investigated the mutagenic potential of simple hydroalcoholic extracts of V. corymbosum acutely administrated by gavage to Swiss mice at doses of 1 g kg-1, 1.5 g kg-1 and 2 g kg-1. Peripheral blood cells were collected 4 h and 24 h post-gavage and assessed by the alkaline comet assay, with further blood samples being collected at 48 h and 72 h for assessment using the micronucleus (MN) assay. Our results show that the V. corymbosum extracts did not induce any statistically significant increase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; Micronucleus; Mutagenicity; Vaccinium corymbosum.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300025

Profile of micronucleus frequencies and DNA damage in different species   of fish in a eutrophic tropical lake
Provedor de dados: 74
Autores: Grisolia,Cesar K.; Rivero,Carla L. G.; Starling,Fernando L. R. M.; Silva,Izabel C. R. da; Barbosa,Antonio C.; Dorea,Jose G.
Lake Paranoá is a tropical reservoir for the City of Brasilia, which became eutrophic due to inadequate sewage treatment associated with intensive population growth. At present, two wastewater treatment plants are capable of processing up to 95% of the domestic sewage, thereby successfully reducing eutrophization. We evaluated both genotoxic and cytotoxic parameters in several fish species ( Geophagus brasiliensis ,  Cichla temensis ,  Hoplias malabaricus ,  Astyanax bimaculatus lacustres ,  Oreochromis niloticus ,  Cyprinus carpio  and  Steindachnerina insculpita ) by using the micronucleus (MN) test, the comet assay and nuclear abnormality assessment in peripheral erythrocytes. The highest frequencies of MN were found in  Cichla temensis  and  Hoplias...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxicity; Fish biomonitoring; Eutrophic lake; Micronucleus; Comet assay; Cytotoxicity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100021

The pequi pulp oil (Caryocar brasiliense Camb.) provides protection against aging-related anemia, inflammation and oxidative stress in Swiss mice, especially in females
Provedor de dados: 74
Autores: Roll,Mariana Matos; Miranda-Vilela,Ana Luisa; Longo,João Paulo Figueiró; Agostini-Costa,Tania da Silveira; Grisolia,Cesar Koppe.
Abstract Continued exposure to reactive oxygen species and inflammation are the rationale behind aging theories and associated diseases. Scientific evidence corroborates the ethnomedicinal use of the oil of pequi (Caryocar brasiliense Camb.), a typical Brazilian Cerrado fruit, against oxidative damage to biomolecules and inflammation. We aimed to investigate in vivo the antioxidant and anti-inflammatory effects of pequi oil on hemogram and DNA damage in healthy young adult and older middle-aged Swiss mice of both genders. Animals, aged 6-7 and 11-12 months, were orally treated for 15 days with pequi oil at 30 mg/day. Blood samples were used for hemogram and comet assay, and bone marrow for micronucleus test. Female controls of 11-12 months had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging-related inflammation and oxidative stress; Comet assay; Micronucleus; Antioxidant; Folk medicine.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500858

Antigenotoxicity and antimutagenicity of ethanolic extracts of Brazilian green propolis and its main botanical source determined by the Allium cepa test system
Provedor de dados: 74
Autores: Roberto,Matheus Mantuanelli; Jamal,Cláudia Masrouah; Malaspina,Osmar; Marin-Morales,Maria Aparecida.
Abstract Brazilian green propolis is a resinous substance prepared by bees from parts of the plant Baccharis dracunculifolia. As it possess several biological properties, this work assessed the cytotoxic/anticytotoxic, genotoxic/antigenotoxic and mutagenic/antimutagenic potential of ethanolic extracts of Brazilian green propolis (EEGP) and of B. dracunculifolia (EEBD), by means of the Allium cepa test system. The effects were evaluated by assessing the chromosomal aberrations (CA) and micronuclei (MN) frequencies on meristematic and F1 generation cells from onion roots. Chemical analyses performed with the extracts showed differences in flavonoid quality and quantity. No genotoxic or mutagenic potential was detected, and both extracts were capable of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal aberration; Micronucleus; Anticlastogenicity; Protective effects; Flavonoids.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200257

Sewage sludge does not induce genotoxicity and carcinogenesis
Provedor de dados: 74
Autores: Silva,Paula Regina Pereira; Barbisan,Luis Fernando; Dagli,Maria Lúcia Zaidan; Saldiva,Paulo Hilário Nascimento.
Through a series of experiments, the genotoxic/mutagenic and carcinogenic potential of sewage sludge was assessed. Male Wistar rats were randomly assigned to four groups: Group 1 - negative control; Group 2 - liver carcinogenesis initiated by diethylnitrosamine (DEN; 200 mg/kg i.p.); Group 3 and G4- liver carcinogenesis initiated by DEN and fed 10,000 ppm or 50,000 ppm of sewage sludge. The animals were submitted to a 70% partial hepatectomy at the 3rd week. Livers were processed for routine histological analysis and immunohistochemistry, in order to detect glutathione S-transferase positive altered hepatocyte foci (GST-P+ AHF). Peripheral blood samples for the comet assay were obtained from the periorbital plexus immediately prior to sacrificing....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; Micronucleus; Sewage genotoxicity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400017

Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips
Provedor de dados: 74
Autores: Matsumoto,Silvia Tamie; Mantovani,Mário Sérgio; Malaguttii,Mirtis Irene Ariza; Dias,Ana Lúcia; Fonseca,Inês Cristina; Marin-Morales,Maria Aparecida.
Cytotoxicity of metals is important because some metals are potential mutagens able to induce tumors in humans and experimental animals. Chromium can damage DNA in several ways, including DNA double strand breaks (DSBs) which generate chromosomal aberrations, micronucleus formation, sister chromatid exchange, formation of DNA adducts and alterations in DNA replication and transcription. In our study, water samples from three sites in the Córrego dos Bagres stream in the Franca municipality of the Brazilian state of São Paulo were subjected to the comet assay and micronucleus test using erythrocytes from the fish Oreochromis niloticus. Nuclear abnormalities of the erythrocytes included blebbed, notched and lobed nuclei, probably due to genotoxic chromium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; Chromium; Chromosomes aberrations; Comet assay; Micronucleus; Oreochromis niloticus.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100028

Mutagenic effects of tributyltin and inorganic lead (Pb II) on the fish H. malabaricus as evaluated using the comet assay and the piscine micronucleus and chromosome aberration tests
Provedor de dados: 74
Autores: Ferraro,Marcos Vinícius M.; Fenocchio,Alberto Sérgio; Mantovani,Mario Sérgio; Ribeiro,Ciro de Oliveira; Cestari,Marta Margarete.
Genotoxicity studies on toxic metals and their organic compounds are very important, especially so in the investigation of the effects of these compounds on the aquatic environments where they tend to accumulate. The use of endemic aquatic organisms as biological sentinels has proved useful to environmental monitoring. We assessed the mutagenic potential of tributyltin (TBT) and inorganic lead (PbII) using samples of the fish Hoplias malabaricus (commonly called traíra) using the comet assay and the piscine micronucleus and chromosome aberration tests. Eighteen H. malabaricus were acclimatized in three individual aquariums, each containing six fish, six fish being exposed to 0.3 mg/g of body weight (bw) of TBT, six to 21 mg/g bw of PbII and six being used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; Micronucleus; Chromosome aberrations; Tributyltin; Lead; Fish.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100017

Protective effect of Hemidesmus indicus R.Br. root extract against cisplatin-induced cytogenetic damage in mouse bone marrow cells
Provedor de dados: 74
Autores: Ananthi,Rajagopal; Chandra,Nallathambi; Santhiya,Sathiyavedu T.
The aqueous extract of Hemidesmus indicus roots was investigated for its in vivo antigenotoxic effect against cisplatin-induced cytogenetic damage. Swiss albino mice were administered with various doses of the extract either singly (50, 100 and 200 mg/kg body weight) or as split doses (10, 20 and 40 mg/kg bw/day) for five consecutive days by oral gavage. As endpoints, chromosome aberrations, micronuclei in polychromatic erythrocytes, mitotic index and PCE/NCE ratio were estimated. The extract protected the bone marrow cells from cisplatin-induced genotoxicity in an inverse dose-dependent manner. However, the extract was cytotoxic at all doses. But, under split dose regime it conferred a higher level of genoprotection and was not cytotoxic at the lower two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genoprotective; Cytotoxic; Chromosomal aberrations; Micronucleus; Mitotic index.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100030

Association between cervical lesion grade and micronucleus frequency in the Papanicolaou test
Provedor de dados: 74
Autores: Bueno,Caroline Tanski; Silva,Cláudia Maria Dornelles da; Barcellos,Regina Bones; Silva,Juliana da; Santos,Carla Rossana dos; Menezes,João Evangelista Sampaio; Menezes,Honório Sampaio; Rossetti,Maria Lucia Rosa.
The aim of this study was to evaluate the association between the frequency of micronuclei (MN) and the cellular changes detected in the conventional Papanicolaou test. One hundred and seventy-four Papanicolaou test smears with cellular changes were examined. MN screening was done in cytopathological smears by counting 1,000 cervical cells in a light microscope. MN frequencies were significantly higher in the group with cellular changes compared to the control group (p &lt; 0.001). The mean MN frequencies were 0.95 ± 1.12 (mean ± SD) in the control group (n = 223), 2.98 ± 1.20 in individuals with atypical squamous cells of undetermined significance (ASC-US) (n = 50), 4.04 ± 1.45 in cervical intraepithelial neoplasia (CIN) I (n = 52), 5.97 ± 1.83 in CIN II...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cervical cancer; Cervical lesions; Micronucleus; Papanicolaou test.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400004

Anticlastogenic potential and antioxidant effects of an aqueous extract of pulp from the pequi tree (Caryocar brasiliense Camb)
Provedor de dados: 74
Autores: Khouri,Juliana; Resck,Inês S.; Poças-Fonseca,Marcio; Sousa,Thiago M.M.; Pereira,Luciana O.; Oliveira,Adriano B.B.; Grisolia,Cesar K.
The effectiveness of an aqueous extract of Caryocar brasiliense (Caryocaraceae) Camb pulp, popularly known in Brazil as pequi, against clastogenicity induced by cyclophosphamide and bleomycin was evaluated using an in vivo mouse bone marrow cell micronuclei test, an in vitro Chinese hamster ovary cell (CHO-K1) chromosome aberration test and an in vitro antioxidant assay based on the oxidative damage to 2-deoxy-D-ribose (2-DR) induced by hydroxyl radicals (•OH) generated by the reaction between ascorbic acid and (Fe III)-EDTA. In mouse bone marrow cells the extract showed a protective effect against micronuclei induced by cyclophosphamide and bleomycin but did not interfere with polychromatic bone marrow erythrocyte proliferation, except when the mice had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caryocar brasiliense; Anticlastogenicity; Antioxidant; Micronucleus.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300024

Absence of mutagenicity in somatic and germ cells of mice submitted to subchronic treatment with an extract of Croton cajucara Benth. (Euphorbiaceae)
Provedor de dados: 74
Autores: Santos,Fabio V.; Mesquita,Suzana F.P.; Faria,Maria José S.S.; Poersh,Aline; Maciel,Maria Aparecida M.; Pinto,Angelo C.; Morimoto,Helena K.; Cólus,Ilce Mara S.
The plant Croton cajucara Benth. (Euphorbiaceae) is a medicinal plant from the Brazilian Amazon where it is commonly known as sacaca. The principal compound isolated from C. cajucara stem-bark extracts is the clerodane-type diterpene trans-dehydrocrotonin (DCTN) which presents several biological activities, including antiulcerogenic, anti-inflammatory, hypoglycemic, antimutagenic and antitumoral activity. However, few studies have been carried out to evaluate the therapeutic potential of raw C. cajucara extracts. We studied mutagenicity and antimutagenicity effects of C. cajucara methanol extract using the micronucleus assay in bone marrow cells and the dominant lethal assay in mice submitted to subchronic treatments. The blood testosterone levels of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimutagenicity; Mutagenicity; Dominant lethal; Micronucleus; Croton cajucara; Medicinal plant.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100029

Genotoxic evaluation of an industrial effluent from an oil refinery using plant and animal bioassays
Provedor de dados: 74
Autores: Rodrigues,Fernando Postalli; Angeli,José Pedro Friedmann; Mantovani,Mário Sérgio; Guedes,Carmen Luisa Barbosa; Jordão,Berenice Quinzani.
Polycyclic aromatic hydrocarbons (PAHs) are genotoxic chemicals commonly found in effluents from oil refineries. Bioassays using plants and cells cultures can be employed for assessing environmental safety and potential genotoxicity. In this study, the genotoxic potential of an oil refinery effluent was analyzed by means of micronucleus (MN) testing of Alium cepa, which revealed no effect after 24 h of treatment. On the other hand, primary lesions in the DNA of rat (Rattus norvegicus) hepatoma cells (HTC) were observed through comet assaying after only 2 h of exposure. On considering the capacity to detect DNA damage of a different nature and of these cells to metabolize xenobiotics, we suggest the association of the two bioassays with these cell types,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; HTC cells; Micronucleus; Comet assay; Petroleum waste disposal.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100028

Cardanol: toxicogenetic assessment and its effects when combined with cyclophosphamide
Provedor de dados: 74
Autores: Schneider,Beatriz Ursinos Catelan; Meza,Alisson; Beatriz,Adilson; Pesarini,João Renato; Carvalho,Pamela Castilho de; Mauro,Mariana de Oliveira; Karaziack,Caroline Bilhar; Cunha-Laura,Andréa Luiza; Monreal,Antônio Carlos Duenhas; Matuo,Renata; Lima,Dênis Pires de; Oliveira,Rodrigo Juliano.
Abstract Cardanol is an effective antioxidant and is a compound with antimutagenic and antitumoral activity. Here, we evaluated the genotoxic and mutagenic potential of saturated side chain cardanol and its effects in combination with cyclophosphamide in preventing DNA damage, apoptosis, and immunomodulation. Swiss mice were treated with cardanol (2.5, 5 and 10 mg/kg) alone or in combination with cyclophosphamide (100 mg/kg). The results showed that cardanol is an effective chemopreventive compound, with damage reduction percentages that ranged from 18.9 to 31.76% in the comet assay and from 45 to 97% in the micronucleus assay. Moreover, cardanol has the ability to reduce the frequency of apoptosis induced by cyclophosphamide. The compound did not show...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenolic lipid; Antimutagenesis; Micronucleus; Comet assay; Apoptosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200279

Antigenotoxic activity and antioxidant properties of organic and aqueous extracts of pequi fruit (Caryocar brasiliense Camb.) pulp
Provedor de dados: 74
Autores: Miranda-Vilela,Ana L.; Resck,Inês S.; Grisolia,Cesar K.
The daily consumption of natural antioxidants protects against oxidative damage caused by reactive oxygen species (ROS), including DNA damage, and can reduce the risk of cancer, atherosclerosis and other degenerative diseases. The pulp of pequi (Caryocar brasiliense Camb.) fruit, a tree native to the Brazilian savannah, contains several compounds with antioxidant properties, including carotenoids, vitamin C, phenolic compounds such as flavonoids, saponins and tannins, and essential oils. In this work, we examined the ability of organic and aqueous extracts of pequi fruit pulp to protect against the genotoxicity induced by two antineoplastic drugs, cyclophosphamide (CP) and bleomycin (BLM). Micronucleus tests with mouse bone marrow cells and single-cell gel...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antigenotoxic; Antioxidant; Caryocar brasiliense; Comet assay; Micronucleus; Pequi pulp extracts; Reactive oxygen species; TBARS assay.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500025

Genotoxicity biomonitoring of sewage in two municipal wastewater treatment plants using the Tradescantia pallida var. purpurea bioassay
Provedor de dados: 74
Autores: Thewes,Márcia Regina; Endres Junior,Delio; Droste,Annette.
The genotoxicity of untreated and treated sewage from two municipal wastewater treatment plants (WTP BN and WTP SJN) in the municipality of Porto Alegre, in the southern Brazilian state of Rio Grande do Sul, was evaluated over a one-year period using the Tradescantia pallida var. purpurea (Trad-MCN) bioassay. Inflorescences of T. pallida var. purpurea were exposed to sewage samples in February (summer), April (autumn), July (winter) and October (spring) 2009, and the micronuclei (MCN) frequencies were estimated in each period. The high genotoxicity of untreated sewage from WTP BN in February and April was not observed in treated sewage, indicating the efficiency of treatment at this WTP. However, untreated and treated sewage samples from WTP SJN had high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Effluent; Micronucleus; Mutagenicity; Pollution; Trad-MCN.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400023

Chromosomal damage and apoptosis analysis in exfoliated oral epithelial cells from mouthwash and alcohol users
Provedor de dados: 74
Autores: Rocha,Rodrigo dos Santos; Meireles,José Roberto Cardoso; Cerqueira,Eneida de Moraes Marcílio.
Chromosomal damage and apoptosis were analyzed in users of mouthwash and/or alcoholic beverages, using the micronucleus test on exfoliated oral mucosa cells. Samples from four groups of 20 individuals each were analyzed: three exposed groups (EG1, EG2 and EG3) and a control group (CG). EG1 comprised mouthwash users; EG2 comprised drinkers, and EG3 users of both mouthwashes and alcoholic beverages. Cell material was collected by gently scraping the insides of the cheeks. Then the cells were fixed in a methanol/acetic acid (3:1) solution and stained and counterstained, respectively, with Schiff reactive and fast green. Endpoints were computed on 2,000 cells in a blind test. Statistical analysis showed that chromosomal damage and apoptosis were significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micronucleus; Apoptosis; Mouthwash; Alcoholic beverages.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500013

Association between human papillomavirus (HPV) DNA and micronuclei in normal cervical cytology
Provedor de dados: 74
Autores: Cassel,Ana Paula Rebelo; Barcellos,Regina Bones; Silva,Cláudia Maria Dornelles da; Almeida,Sabrina Esteves de Matos; Rossetti,Maria Lucia Rosa.
The aim of the present study was to investigate the association between HPV-DNA and micronucleus (MN) frequency in women with normal cervical cytology. A total of 158 normal cervical smears were analyzed cytologically. The HPV genome was amplified using the GP5+/bioGP6+ consensus primers. HPV-DNA of high-risk types 16, 18, 31, 33, 39, 45 and 59 were also investigated. Of the 158 samples, 20 (12.7%) and 47 (29.7%) were positive for HPV-DNA and MN, respectively. Evidence for MN was found in 11 out of 20 (55%) HPV-DNA positive samples and in 36 out of 138 (26.1%) HPV-DNA negative ones. MN presence was significantly higher in HPV-DNA positive samples (p = 0.016). On the other hand, the absence of MN observed in a considerable number of HPV-DNA negative samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Micronucleus; Cytology; High-risk HPV; Southern Brazil.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300007

Cytotoxic and genotoxic potential of surface water from the Pitimbu river, northeastern/RN Brazil
Provedor de dados: 74
Autores: Egito,Lucila Carmem Monte; Medeiros,Maria das Graças; Medeiros,Silvia Regina Batistuzzo de; Agnez-Lima,Lucymara Fassarella.
In this study, the onion (Allium cepa) root test was used to evaluate the genotoxicity of the Pitimbu River (Natal city, Brazil) surface water. The water was collected at five sampling sites along the river and one sample was obtained after the treatment (flocculation, chlorination and pH correction) of the river water for human consumption. All raw river water samples increased the frequency of chromosomal abnormalities and/or micronuclei and two of the water samples produced alterations in the mitotic index of the root cells. Two of the water samples also altered root growth and two produced morphological modifications in the A. cepa roots. Water collected from a site near an industrial area was the most consistently toxic and genotoxic of the samples....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; Micronucleus; Cytotoxicity; Genotoxicity; Surface water.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300023

Genotoxicity assessment of a pharmaceutical effluent using four bioassays
Provedor de dados: 74
Autores: Bakare,Adekunle A.; Okunola,Alabi A.; Adetunji,Olusanmi A.; Jenmi,Hafeez B.
Pharmaceutical industries are among the major contributors to industrial waste. Their effluents when wrongly handled and disposed of endanger both human and environmental health. In this study, we investigated the potential genotoxicity of a pharmaceutical effluent, by using the Allium cepa, mouse- sperm morphology, bone marrow chromosome aberration (CA) and micronucleus (MN) assays. Some of the physico-chemical properties of the effluent were also determined. The A. cepa and the animal assays were respectively carried out at concentrations of 0.5, 1, 2.5, 5 and 10%; and 1, 5, 10, 25 and 50% of the effluent. There was a statistically different (p &lt; 0.05), concentration-dependent inhibition of onion root growth and mitotic index, and induction of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxicity; Pharmaceutical effluent; Mouse; Allium cepa; Chromosome; Spermatozoa; Micronucleus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200026

Cytotoxicity and genotoxicity of Agaricus blazei methanolic extract fractions assessed using gene and chromosomal mutation assays
Provedor de dados: 74
Autores: Bellini,Marilanda Ferreira; Cabrioti,Leonardo Neves; Terezan,Ana Paula; Jordão,Berenice Quinzani; Ribeiro,Lúcia Regina; Mantovani,Mário Sérgio.
Functional food investigations have demonstrated the presence of substances that could be beneficial to human health when consumed. However, the toxic effects of some substances contained in foods have been determined. Reported medicinal and nutritive properties have led to the extensive commercialization of the basidiomycete fungi Agaricus blazei Murrill (sensu Heinemann), also known as Agaricus brasiliensis Wasser et al., Agaricus subrufescens Peck or the Brazilian medical mushroom (BMM). Different methanolic extract fractions (ME) of this mushroom were submitted to the cytokinesis-block micronucleus (CBMN) clastogenic assay and the hypoxanthine-guanine phosphoribosyl transferase locus (HGPRT) assay for gene mutation, both using Chinese hamster ovary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agaricus blazei; Hgprt locus; Micronucleus.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100021

Effects of β-glucan polysaccharide revealed by the dominant lethal assay and micronucleus assays, and reproductive performance of male mice exposed to cyclophosphamide
Provedor de dados: 74
Autores: Oliveira,Rodrigo Juliano; Pesarini,João Renato; Salles,Maria José Sparça; Kanno,Tatiane Yumi Nakamura; Lourenço,Ana Carolina dos Santos; Leite,Véssia da Silva; Silva,Ariane Fernanda da; Matiazi,Hevenilton José; Ribeiro,Lúcia Regina; Mantovani,Mário Sérgio.
β-glucan is a well-known polysaccharide for its chemopreventive effect. This study aimed to evaluate the chemopreventive ability of β-glucan in somatic and germ cells through the dominant lethal and micronucleus assays, and its influence on the reproductive performance of male mice exposed to cyclophosphamide. The results indicate that β-glucan is capable of preventing changes in DNA in both germ cells and somatic ones. Changes in germ cells were evaluated by the dominant lethal assay and showed damage reduction percentages of 46.46% and 43.79% for the doses of 100 and 150 mg/kg. For the somatic changes, evaluated by micronucleus assay in peripheral blood cells in the first week of treatment, damage reduction percentages from 80.63-116.32% were found. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mutation; Post-implantation losses; Chemoprevention; Micronucleus; Nulliparous females.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100017

Toxicity and genotoxicity of wastewater from gasoline stations
Provedor de dados: 74
Autores: Oliveira-Martins,Cynthia R.; Grisolia,Cesar K.
The toxicity and genotoxicity of wastewater from eight gasoline stations in Brasília, Brazil's capital city, was studied by assessing chromosomal aberrations, chromosomal malsegregation and the mitotic index in Allium cepa root cells, and the occurrence of micronucleus and nuclear abnormalities in peripheral erythrocytes of tilapia fish (Oreochromis niloticus). The content of gasoline station effluents was also analyzed based on several physico-chemical parameters. None of the wastewater samples was genotoxic to A. cepa root cells, although cell proliferation was significantly inhibited, especially at the highest concentrations. Likewise, no micronuclei were observed in O. niloticus peripheral erythrocytes, even after exposure to high concentrations, but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; Chromosomal aberrations; Gas station; Micronucleus; Oreochromis niloticus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400029

Protective effects of acerola juice on genotoxicity induced by iron in vivo
Provedor de dados: 74
Autores: Horta,Roberta Nunes; Kahl,Vivian Francilia Silva; Sarmento,Merielen da Silva; Nunes,Marisa Fernanda Silva; Porto,Carem Rejane Maglione; Andrade,Vanessa Moraes de; Ferraz,Alexandre de Barros Falcão; Silva,Juliana Da.
Abstract Metal ions such as iron can induce DNA damage by inducing reactive oxygen species (ROS) and oxidative stress. Vitamin C is one of the most widely consumed antioxidants worldwide, present in many fruits and vegetables, especially inMalpighia glabra L., popularly known as acerola, native to Brazil. Acerola is considered a functional fruit due to its high antioxidant properties and phenolic contents, and therefore is consumed to prevent diseases or as adjuvant in treatment strategies. Here, the influence of ripe and unripe acerola juices on iron genotoxicity was analyzed in vivo using the comet assay and micronucleus test. The comet assay results showed that acerola juice exerted no genotoxic or antigenotoxic activity. Neither ripe nor unripe acerola...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxicity; Comet assay; Micronucleus; Acerola juice; Iron.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100122

Genotoxic evaluation of the River Paranaíba hydrographic basin in Monte Carmelo, MG, Brazil, by the Tradescantia micronucleus
Provedor de dados: 74
Autores: Campos,Carlos F.; Pereira,Boscolli B.; Campos-Junior,Edimar O. de; Sousa,Eduardo F.; Souto,Henrique N.; Morelli,Sandra.
Abstract Pollutants have adverse effects on human health and on other organisms that inhabit or use water resources. The aim of the present study was to assess the environmental quality of three watercourses in Monte Carmelo, MG, Brazil, using the micronucleus test on Tradescantia. For each treatment, 15 plants were exposed to water samples for 24 h. The control group was exposed to formaldehyde (0.2%) and the negative control to Hoagland solution. Subsequently the plants were placed in Hoagland solution for 24 h to recover. Cells were stained with 2% acetic carmine and examined by light microscopy. Three hundred tetrads were analyzed per slide. The frequency of genotoxic alterations was expressed as the number of micronuclei per 100 tetrads, and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomonitoring; Genotoxicity; Micronucleus; Tradescantia pallida.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400507

Genotoxicity assessment of Copaiba oil and its fractions in Swiss mice
Provedor de dados: 74
Autores: Almeida,Mara Ribeiro; Darin,Joana D'Arc Castania; Hernandes,Lívia Cristina; Ramos,Mônica Freiman de Souza; Antunes,Lusânia Maria Greggi; Freitas,Osvaldo de.
Copaiba oil-resin, extracted from the trunk of Copaifera, and traditionally used in folk medicine in the treatment of various disorders, has been shown to be an effective antiinflamatory, antitumor, antitetanus, antiseptic and antiblenorrhagea agent. As, there are few studies evaluating its genotoxicity, this aspect of the commercial oil-resin, and its volatile and resinous fractions, were evaluated in mice by comet assay and micronucleus (MN) test. A single dose of oil resin, volatile or resin fractions (500; 1,000 or 2,000 mg/kg b.w.) was administered by gavage. The chemical compositions of Copaiba oil resin and its fractions was analyzed by gas chromatography. According to comet assaying, treatment with either one did not increase DNA damage, and as to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Copaiba oilresin; Genotoxicity; Comet assay; Micronucleus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400018

Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae)
Provedor de dados: 83
Autores: Santos,Celina Alcoforado; Lenz,Dominik; Brandão,Geisamanda Pedrini; Chippari-Gomes,Adriana Regina; Gomes,Levy Carvalho.
Diesel oil can be a source of contamination in aquatic environments, mainly as a result of spills. The effects of the water-soluble fraction of diesel (WSF) on Prochilodus vimboideswere assessed. Fish were exposed to three different WSF dilutions for up to 96 h and were compared to a control group. Damages in the fragments of DNA were analyzed using the Comet assay. The presence of erytrocyts abnormalities was assessed by micronucleus test. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity and the accumulation of copper in gills were also analyzed. Fish exposed for 96 h had higher rates of damage than those exposed for 24 h. There was no significant difference regarding the presence of micronuclei between exposed and control fish...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; Curimba; Diesel; Micronucleus.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000100193

Influence of natural radon and metal contamination on surface water quality from a Brazilian Semiarid Region
Provedor de dados: 3
Autores: Dantas, Richelly da Costa; Navoni, Julio Alejandro; Ferreira, Douglisnilson de Morais; Costa, Thomas Ferreira da; Medeiros, Silvia Regina Batistuzzo de; Amaral, Viviane Souza do.
The Brazilian semiarid region presents some adverse environmental conditions for the settled population such as a restricted water availability and the presence of radon and metal natural sources that can contaminate the water reservoirs and consequently become a concern for human health. The present study evaluated the water quality of the Riacho das Cachoeiras Dam located in the urban area of Lajes Pintadas (state of Rio Grande do Norte, Brazil) as source for human consumption. An analysis of Physicochemical parameters, heavy metal content and Radon in water samples was performed along with the assessment of the water mutagenic potential through Micronucleus Test (MN) on Tradescantia pallida and Oreochromis niloticus. The content of metals in water for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Area: Genetica. Sub-area: Mutagenese radiation; Lead; Mutagenicity; Micronucleus; Geogenic contamination. Genetica.
Ano: 2017
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/27787

Comparison of nuclear abnormalities in Astyanax bifasciatus Cuvier, 1819 (Teleostei: Characidae) of two sections of rivers from the middle Iguaçu
Provedor de dados: 3
Autores: Melo-Silva, Merieli; Oliveira, Felipe Rafael; Rosa, Jonathan; Gemelli, Emanuelli; Santos, Lilian; Bueno-Krawczyk, Ana Carolina de Deus.
Most changes in water bodies are result of human activities that have the potential to undermine the environmental integrity of aquatic ecosystems. Changes in genetic material can be evidenced by the frequency of nuclear abnormalities in fish blood cells, in response to genotoxic agents even at low concentrations. Thus, we aimed at comparing the frequencies of nuclear abnormalities of fish kept in acclimation, with fish collected in Timbó river (Santa Cruz do Timbó, Santa Catarina State), under low anthropogenic interference, and fish collected in Iguaçu river (União da Vitória, Paraná State), a polluted river. The highest frequency of changes in nuclear morphology of fish erythrocytes was found in the urbanized area around the Iguaçu river, while, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micronucleus; Ecotoxicology; Mutagenesis; Pollution. Mutagênese.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/40669

Genotoxic and Genoprotective Potential of Black Mulberry (Morus nigra) Fruit
Provedor de dados: 42
Autores: YILMAZ,SERKAN; UÇAR,ASLI; GÖKTAŞ,BAYRAM.
Abstract: Black Mulberry (Morus nigra L.) belongs to Moraceae family. The present study evaluated the possible genotoxic and/or protective activities of black mulberry fruit juice (BMFJ), in vitro, using mitomycin C (MMC) as a positive control, by chromosomal aberrations and micronucleus assays. Human lymphocytes were treated with BMFJ concentrations alone (1/1, 1/2, 1/4, 1/8 dilutions), pretreatment (49h) (0.20 μg/ml MMC+ 1/1 BMFJ, 0.20 μg/ml MMC+1/2 diluted BMFJ, 0.20 μg/ml MMC+1/4 diluted BMFJ, 0.20 μg/ml MMC+1/8 diluted BMFJ) and simultaneous-treatment (48h) (0.20 μg/ml MMC+ 1/1 BMFJ, 0.20 μg/ml MMC+1/2 diluted BMFJ, 0.20 μg/ml MMC+1/4 diluted BMFJ, 0.20 μg/ml MMC+1/8 diluted BMFJ). The in vitro results demonstrated that BMFJ showed no genotoxicity,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Black mulberry; Chromosomal aberrations; Micronucleus; Genotoxicity; Anti-genotoxicity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700602

Antigenotoxicity protection of Carapa guianensis oil against mitomycin C and cyclophosphamide in mouse bone marrow
Provedor de dados: 42
Autores: LEMES,SUSY R.; CHAVES,DWIGHT A.; SILVA JÚNIOR,NELSON J. DA; CARNEIRO,CRISTIENE C.; CHEN-CHEN,LEE; ALMEIDA,LUCIANE M. DE; GONÇALVES,PABLO J.; MELO-REIS,PAULO R. DE.
ABSTRACT The aim of this study was to evaluate the possible protective of C. guianensis oil against MMC and CP, which are direct- and indirect-acting chemical mutagens, using the micronucleus test. Three experiments were performed. First the C. guianensis oil was co-administered to mice at doses of 250, 500 and 1000 mg/kg bw with 4 mg/kg bw MMC or 50 mg/kg bw CP. Second, the mutagenic drug (CP) was administered ip 50 mg/kg bw and after 6 and 12 hours 250 and 500 mg/kg bw of C. guianensis oil were administered. In the last, C. guianensis oil was administrated (250 and 500 mg/kg bw) during five days and after it was administered ip 50 mg/kg bw CP. The results obtained showed that the C. guianensis oil is not cytotoxic neither genotoxic to mouse bone marrow....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antigenotoxic; Carapa guianensis; Micronucleus; Oil.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502043

DNA damage and cytotoxicity in pathology laboratory technicians exposed to organic solvents
Provedor de dados: 42
Autores: AQUINO,TATIANE DE; ZENKNER,FERNANDA F.; ELLWANGER,JOEL H.; PRÁ,DANIEL; RIEGER,ALEXANDRE.
The aim of this study was to evaluate potential DNA damage and cytotoxicity in pathology laboratory technicians exposed to organic solvents, mainly xylene. Peripheral blood and buccal cells samples were collected from 18 technicians occupationally exposed to organic solvents and 11 non-exposed individuals. The technicians were sampled at two moments: Monday and Friday. DNA damage and cytotoxicity were evaluated using the Comet Assay and the Buccal Micronucleus Cytome assay. Fifteen subjects (83.5%) of the exposed group to solvents complained about some symptom probably related to contact with vapours of organic solvents. DNA damage in the exposed group to solvents was nearly 2-fold higher on Friday than on Monday, and in both moments the individuals of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA damage; Micronucleus; Toxicology; Occupational health; Organic solvents; Xylene.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100227

High consumption of sucrose induces DNA damage in male Wistar rats
Provedor de dados: 42
Autores: FRANKE,SILVIA I.R.; MOLZ,PATRÍCIA; MAI,CAMILA; ELLWANGER,JOEL H.; ZENKNER,FERNANDA F.; HORTA,JORGE A.; PRÁ,DANIEL.
ABSTRACT The purpose of this study was to determine the effects of the high consumption of sucrose on the levels of DNA damage in blood, hippocampus and bone marrow of rats. Male Wistar rats were treated for 4 months with sucrose (10% for 60 initial days and 34% for the following 60 days) in drinking water, and then, glycemia and glycated hemoglobin (A1C) were measured. Levels of DNA damage in blood and hippocampus were evaluated by the comet assay. The micronucleus test was used to evaluate chromosomal damages in the bone marrow. The sucrose treatment significantly increased (p&lt;0.01) the serum glucose levels (~20%) and A1C (~60%). The level of primary DNA damage was significantly increased (p&lt;0.05) in hippocampal cells (~60%) but not in peripheral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; Diabetes; DNA damage; Glycated hemoglobin; Micronucleus; Sucrose.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602657

In Vitro Genotoxic Effects of Four Helichrysum Species in Human Lymphocytes Cultures
Provedor de dados: 48
Autores: Erolu,Erhan H; Hamzaolu,Ergin; Aksoy,Ahmet; Budak,Ümit; Özkul,Yusuf.
Helichrysum sanguineum, Helichrysum pamphylicum, Helichrysum orientale, Helichrysum noeanum (Asteraceae) are medicinal plants. For centuries, they have been used as tea in Turkey because of their medicinal properties. So far no scientifc evidence has been found in a literature survey regarding the genotoxic effects of these plants. This work evaluated the genotoxic effects on human lymphocyte cultures induced by methanol extracts of these plants, assayed in different concentrations (0.01, 0.05, 0.1, 0.5 and 1 mg/mL). According to the results, Helichrysum noeanum, Helichrysum pamphylicum and Helichrysum sanguineum induced the formation of micronuclei and decreased the mitotic and replication indexes. Helichrysum orientale did not affect these parameters,...
Tipo: Journal article
Palavras-chave: Genotoxic; Helichrysum; Micronucleus; Mitotic index; Replication index.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200005

Microscopic Studies on Erythrocytes of Channa punctata Exposed to Commercial Grade Lindane
Provedor de dados: 52
Autores: Bhattacharjee,Debasish; Das,Suchismita.
ABSTRACT Light and scanning electron microscopic studies of erythrocytes of Channa punctata exposed to two sublethal doses of lindane over a period of 21 days revealed myriads of anomalies. High frequency of micronucleus appeared progressively in the erythrocyte, ranging from 3.8-7.5%, when monitored weekly for 21 d at 0.25 g/L and 1.0-3.0% at 0.025 g/L lindane. Prominent structural anomalies of erythrocytes included abnormal shapes, vacuolation, membrane invagination and disintegration. At higher dose and at later duration, a unique tendency of abnormal cells to stick together was observed. It could be concluded that commercial formulation of lindane could induce genotoxicity and structural anomalies in the erythrocytes of fish.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RBC; SEM; Light microscopy; Micronucleus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100449

The cytogenetic effects of black tea and green tea on cultured human lymphocytes
Provedor de dados: 52
Autores: Eroğlu,Halil Erhan.
In this study, the cytogenetic effects of black tea and green tea were determined in cultured peripheral blood lymphocytes. Results showed that black tea and green tea induced the mitotic and replication indexes and decreased micronuclei. But these data were not statistically significant for green tea. The effects of black tea on the micronucleus formation and mitotic index were statistically significant. The decrease in micronucleus counts indicated that black tea and green tea had considerable anticlastogenic and antigenotoxic effects as observed in vitro in human lymphocytes. Thus, it could be concluded that tea polyphenols protected the normal cells from genotoxic or carcinogenic agents, which indicated the therapeutic and antioxidative role of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tea; Catechins; Micronucleus; Mitotic index; Replication index.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600011

Impact of Anthropogenic Activities on Water Quality of The Paranhana River, Southern Brazil
Provedor de dados: 52
Autores: Dalzochio,Thaís; Souza,Mateus Santos de; Simões,Leonardo Airton Ressel; Silva,Gláucia Joselaine Herbert; Rodrigues,Gabriela Zimmermann Prado; Andriguetti,Natália Bordin; Silva,Luciano Basso da; Gehlen,Günther.
Abstract: The Paranhana River, located in Southern Brazil, is one of the major tributaries of the Sinos River basin and receives mainly industrial and domestic effluents. In the present study, water physicochemical and microbiological analyses, condition factor, micronucleus test, gill histopathology and metal bioaccumulation in the muscle of the native fish Bryconamericus iheringii collected at two sites (S1 and S2) of the Paranhana River under different degrees of anthropogenic pressures were assessed in four sampling campaigns. Data from water quality parameters, condition factor, mucous cells proliferation in fish gills and bioaccumulation of chromium and manganese in muscle evidenced higher impacts at S2, whereas a higher genotoxic potential was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomarkers; Paranhana River; Bioaccumulation; Histopathology; Micronucleus.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100421

In Vitro Study Towards Role of Acrylamide-Induced Genotoxicity in Human Lymphocytes and the Protective Effect of L- Carnitine
Provedor de dados: 52
Autores: Zamani,Ehsan; Shokrzadeh,Mohammad; Modanloo,Mona; Shaki,Fatemeh.
ABSTRACT Acrylamide (AA), is an important industrial compound, formed during food processing under high temperature. Due to its potential carcinogenic effect, it has been recognized as a human health concern. In this study, we investigated the role of oxidative stress in acrylamide’s genotoxicity and protective role of L- carnitine (LCA) in human lymphocytes. The micronucleus test (MNT) was used to monitor AA genotoxicity after 20h exposure to concentrations of 0.25,50 and 100 mM. Furthermore we evaluated oxidative stress markers, such as reactive oxygen species (ROS), lipid peroxidation (LPO) and glutathione (GSH) content. In MNT, It has been found that AA at high concentrations (50 and 100 µM) significantly increased the micronuclei (MN) frequencies. On...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acrylamide; L- carnitine; Micronucleus; Oxidative stress; Genotoxicity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100426

Genotoxic activity of the insecticide Nuvacron (Monocrotophos) detected by the micronucleus test in bone marrow erythrocytes of mice and in CHO cells
Provedor de dados: 54
Autores: Peitl Jr,Paulo; Sakamoto-Nojo,Elza T.; Cólus,Ilce M. de Syllos.
The organophosphorus insecticide Nuvacron (Monocrotophos) is a very toxic agent widely utilized in Brazilian agriculture. To evaluate the clastogenic potential of this insecticide, in vivo and in vitro micronucleus (MN) assay experiments were carried out on Swiss mice and on Chinese hamster ovary (CHO) cells, respectively. Nuvacron administered at doses of 2.5 and 5.0 mg/kg induced a statistically significant increase in the frequencies of MN detected in polychromatic bone marrow erythrocytes from animals (six/group) treated ip 24 h before. Exponentially growing CHAO cells were treated continuously (16h) with Nuvacron diluted in water to final concentrations of 1, 10, 100, 200, and 400 mug/ml. Three experiments were carried out using the cytokinesis-block...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxic; Insecticide Nuvacron; Micronucleus; Bone marrow; Erythrocytes; Mice; CHO cells.
Ano: 1996
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400006

Cell cycle synchronization and BrdU incorporation as a tool to study the possible selective elimination of ErbB1 gene in the micronuclei in A549 cells
Provedor de dados: 56
Autores: Lauand,C.; Niero,E.L.; Dias,V.M.; Machado-Santelli,G.M.
Lung cancer often exhibits molecular changes, such as the overexpression of the ErbB1 gene that encodes epidermal growth factor receptor (EGFR). ErbB1 amplification and mutation are associated with tumor aggressiveness and low response to therapy. The aim of the present study was to design a schedule to synchronize the cell cycle of A549 cell line (a non-small cell lung cancer) and to analyze the possible association between the micronuclei (MNs) and the extrusion of ErbB1 gene extra-copies. After double blocking, by the process of fetal bovine serum deprivation and vincristine treatment, MNs formation was monitored with 5-bromo-2-deoxyuridine (BrdU) incorporation, which is an S-phase marker. Statistical analyses allowed us to infer that MNs may arise both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: A549 cells; Cell cycle synchronization; BrdU incorporation; ErbB1; Micronucleus.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500382

Pollen mother cells of Tradescantia clone 4430 and Tradescantia pallida var. purpurea are equally sensitive to the clastogenic effects of X-rays
Provedor de dados: 56
Autores: Suyama,F.; Guimarães,E.T.; Lobo,D.-J.A.; Rodrigues,G.S.; Domingos,M.; Alves,E.S.; Carvalho,H.A.; Saldiva,P.H.N.
The Tradescantia micronucleus test is a sensitive bioassay for mutagenesis that may be employed both under field and laboratory conditions. This test has been standardized mostly on the basis of the results obtained with clone 4430. However, this clone is not well adapted to tropical weather, frequently showing problems with growth and flowering. In addition, it is attacked by parasites and insects, a fact that limits its use in field studies aiming at the biomonitoring of air pollution. In the city of São Paulo, Tradescantia pallida (Rose) Hunt. var. purpurea Boom is widely distributed as an ornamental plant in gardens and along roadsides and streets, mostly because of its natural resistance and its easy propagation. In this report, we present...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tradescantia; Micronucleus; Environmental mutagenesis; Clastogenesis; X-Radiation; Pollution biomonitoring.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100018

Laurus nobilis L.: assessment of the cytotoxic and genotoxic potential of aqueous extracts by micronucleus and Allium cepa assays
Provedor de dados: 60
Autores: Silva,Mayara Christine; Matos,Aline Ferreira; Santos,Hataânderson Luíz Cabral dos; Gomes,Jennifer Vieira; Pastura,Danilo Giovanni Narciso; Pereira,Gabriela Lopes; Rocha,Ellen Borges da; Larangeira,Mayara de Jesus Costa; Alves,Raphael Santiago; Bastos,Leonardo de Oliveira; Borba,Helcio Resende; Lima,Viviane Moreira de.
Laurus nobilis L. is a large shrub belonging to the Lauracea family. Its leaves are widely used for food seasoning as well as in folk medicine. Various studies have demonstrated the antiproliferative, antifungal and antibacterial effects of Laurus nobilis, but no studies have investigated the genotoxic effect of the aqueous extract of the plant. The objective of this study was to analyze the genotoxic potential of an aqueous extract of leaves, using the Allium cepa assay and mouse peripheral blood cell micronucleus test. The results showed that the extract did not have any genotoxic activity, but cytotoxic activity was observed in the two experimental models used. The extract had an antiproliferative effect, detected through the reduction of the mitotic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laurus nobilis; Allium cepa; Micronucleus; Genotoxicity; Cytotoxicity.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100534