Consulta: subjectFacets:"Micropropagation"
Registros recuperados: 113
Data/hora: 03/06/2024 16:42:20

Caracterización molecular de Laelia halbingeriana y su propagación in vitro.
Provedor de dados: 32
Autores: Raya Montaño, Yurixhi Atenea.
Laelia halbingeriana pertenece a la familia Orchidaceae y debido a su descubrimiento relativamente reciente, no se ha caracterizado morfológica, cromosómica ni molecularmente. Con la finalidad de conocer la diversidad de esta especie y su posible relación con L. anceps, L. superbiens y L. autumnalis, la caracterización molecular  mediante el análisis de RAPDs se utilizaron 16 iniciadores, cuatro iniciadores mostraron productos de amplificación aceptable. Se observó 60.97 % de polimorfismo que denota gran variabilidad entre las especies de Laelia. Por otra parte se observó que el número cromosómico más común es de 2n=36. Además, en un tercer estudio se desarrolló una metodología para la propagación in vitro de L. halbingeriana se utilizó el medio de MS...
Palavras-chave: Laelia halbingeriana; Marcadores moleculares; Cromosomas; Micropropagación; Aclimatación; Molecular markers; Chromosomae; Micropropagation; Genética; Doctorado; Acclimation.
Ano: 2013
URL: http://hdl.handle.net/10521/2049

Organogénesis directa de novo en Musa AAA 'Enano gigante' y 'FHIA 23'.
Provedor de dados: 32
Autores: López Barrios, Zeila Yaneth.
Nuestro país se encuentra entre las primeras 12 naciones productoras de banano (Musa sp), y en el ámbito nacional este cultivo ocupa el tercer lugar en importancia, además de estar incluido dentro del rubro de frutas de exportación, que lo convierte en un cultivo altamente rentable. El banano tiene gran demanda en el mercado nacional, con un consumo per cápita de 23 kg anuales, debido a que se encuentra disponible durante todo el año (Daquita, 2006).  La Sigatoka negra (Micosphaerella fijiensis) es la principal enfermedad que limita la producción de este frutal. La transformación genética de genotipos es una técnica de vanguardia exitosa que puede permitir incorporar genes que confieran tolerancia a enfermedades por hongos, una vez que se disponga de un...
Palavras-chave: Organogénesis de novo; Zeatina; Cinetina; Thidiazuron; Micropropagación; Zeatin; Kinetin; Micropropagation; Maestría; Fruticultura.
Ano: 2010
URL: http://hdl.handle.net/10521/343

Cultivo in vitro de Stevia rebaudiana Bertoni.
Provedor de dados: 32
Autores: Vázquez Baxcajay, Liberia.
Stevia  es un género de plantas fanerógamas perteneciente a la familia de las asteráceas, tiene 407 especies; una de sus especies es Stevia rebaudiana Bertoni, nombrada así por Moisés de Santiago Bertoni. Para el establecimiento in vitro de Stevia rebaudiana se aplicaron cinco tratamientos de desinfestación y se obtuvo al usar Cloro® (1.8 % cloro activo), Ridomil y Fungimycin previo a la siembra in vitro y al agregar al medio Cefotaxima (500 mgL-1) se obtuvo un porcentaje de contaminación de 50 % permitiendo una supervivencia de 47 %. En la inducción de callo se mostro que al combinar 1.5 mgL-1 2,4-D/AIA la capacidad de formación de callo del explante de hoja es de 25 %. El tratamiento de cinetina (1.3 gL-1) en el proceso micropropagación de Stevia mostro...
Palavras-chave: In vitro; Micropropagación; Aclimatación; Stevia; Micropropagation; Acclimatization; Maestría; Fisiología Vegetal.
Ano: 2012
URL: http://hdl.handle.net/10521/751

Germinación y propagación de Tillandsia macdougallii L. B Sm. y Tillandsia violacea Baker (Bromeliaceae) con fines de aprovechamiento sustentable.
Provedor de dados: 32
Autores: Vázquez Hurtado, Nora Berenice.
Los procesos sustentables son adoptados con mayor frecuencia por comunidades rurales de México. La conveniencia de conservar y aprovechar de una forma sostenible los recursos naturales, especialmente las bromelias (con potencial ornamental), pueden generar opciones y beneficios para las familias. Como una opción para evitar el saqueo, así como contribuir al manejo y protección, de las bromelias .En esta investigación se plantearon los siguientes objetivo:1) se evaluó el porcentaje de germinación y viabilidad en las semillas de Tillandsia macdougallii y Tillandsia violacea, 2) se probó el efecto de los tratamientos pregerminativos, AG₃ y NKO₃ en la germinación y desarrollo de plántulas, 3) se estudiaron los efectos de diferentes sustratos para el...
Palavras-chave: Germinación; Viabilidad; Sustratos; Micropropagación; Sobrevivencia; Germination; Viability; Substrates; Micropropagation; Survival; Botánica; Maestría.
Ano: 2014
URL: http://hdl.handle.net/10521/2298

Morfogénesis in vitro de cactáceas.
Provedor de dados: 32
Autores: Téllez Román, Janeth.
En esta investigación se estudiaron las condiciones de cultivo in vitro y las respuestas morfogénicas de tres especies de cactáceas: Echinopsis chamaecereus Friedrich &amp; Glaetzle “aurea”, Gymnocalicium mihanovichii cv. Hibotan y Mammillaria plumosa Weber ornamentales de importancia económica y la última en peligro de extinción. Con la regeneración de brotes, se establecieron protocolos eficientes para la propagación masiva in vitro a partir de la vía organogénica directa e indirecta de E. chamaecereus aurea y M. plumosa. Finalmente, se evaluaron varios tipos de uniones para el establecimiento de microinjertos compuestos de estas dos especies (injertos) e Hylocereus undatus (portainjerto). Durante la micropropagación, los explantes utilizados en todas...
Palavras-chave: Cactácea; Micropropagación; Organogénesis indirecta; Organogénesis directa; Microinjertos; Cactus; Micropropagation; Indirect organogenesis; Direct organogenesis; Micrografts; Fisiología Vegetal; Doctorado.
Ano: 2012
URL: http://hdl.handle.net/10521/1680

Gene expression of a putative glutathione S-transferase is responsive to abiotic stress in embryogenic cell cultures of Cyclamen persicum
Provedor de dados: 69
Autores: Hoenemann,Claudia; Ambold,Juliane; Hohe,Annette.
Background: Cyclamen persicum is an economically important ornamental crop that is propagated exclusively through seeds as vegetative propagation using cuttings is not possible. Therefore a micropropagation method through somatic embryogenesis is of high interest; however the method suffers from low reliability concerning quality and quantity of the produced plantlets. A crucial step of the protocol is the removal of plant growth regulators (PGRs) that triggers embryo development. In order to get a better insight in this crucial step of the propagation process, a gene expression analysis has been set up using five different genes of glutathione S-transferases (GST) as these are known to be auxin responsive as well as stress reactive. Results: One out of...
Tipo: Journal article
Palavras-chave: Auxin; Callus; Micropropagation; Semisolid medium; Suspension culture.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100006

Perspectives on the application of biotechnology to assist the genetic enhancement of plantain and banana (Musa spp.)
Provedor de dados: 69
Autores: Crouch,Jonathan H.; Vuylsteke,Dirk; Ortiz,Rodomiro.
Bananas and plantains (Musa spp.) are the most important tropical fruit crops. They form an integral component of the farming systems in the humid agroecological zones of the tropics. A broad array of applied cell and molecular techniques are increasingly being used worldwide to facilitate and enhance the handling and improvement of plantain and banana germplasm. Tissue culture is used for germplasm exchange, conservation and rapid multiplication, while in vitro seed germination (based on embryo culture or rescue) plays a critical role in generating hybrid plants. DNA marker systems have been developed in Musa to assist germplasm management, selection within the breeding pool or gene introgression from wild species, and for disease diagnosis. Likewise,...
Tipo: Journal article
Palavras-chave: Banana; Improvement; Micropropagation; DNA; Markers; Plantain.
Ano: 1998
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000100002

An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis
Provedor de dados: 69
Autores: Sharry,Sandra; Cabrera Ponce,José Luis; Herrera Estrella,Luis; Rangel Cano,Rosa María; Lede,Silvia; Abedini,Walter.
A highly efficient somatic embryogenesis system and subsequent plant regeneration of chinaberry (Melia azedarach L.) was developed. Plants were regenerated from indirect somatic embryogenesis induction. Novel features of this improved protocol, include: a) Embryogenic callus induction with no addition of 2, 4-D in the culture media; b) Somatic embryos differentiation was achieved by using high concentration of cytokinins (BAP 10 mg/L) and adenine; c) 100% conversion of somatic embryos to plants was practically obtained and 100% of plants survived under greenhouse conditions; d) Addition of putrescine improved somatic embryos germination. The amount of somatic embryos produced by the pathway of indirect somatic embryogenesis was 447 per gram of fresh weight...
Tipo: Journal article
Palavras-chave: Callus induction; Clonal forestry; Embryos conversion; Micropropagation; Persian lilac; Somatic embryos.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300003

Establishment of an in vitro micropropagation protocol for Mecardonia tenella
Provedor de dados: 69
Autores: Alderete,Liliana Marisol; Mori,Marcela; Kato,Adriana; Salvio Escandón,Alejandro.
Mecardonia tenella is an attractive herbaceous native plant, characterized by their intense green foliage and their abundant yellow flowers. It is a very interesting plant for pot and/or garden. A trait to improve in this species is the size of the flowers. The goal of the present paper is to study the in vitro behaviour of M. tenella and to establish a routine protocol for its micropropagation. For the in vitro establishment of M. tenella, nodal segments were disinfected by standard methods using ethanol/sodium hypochloride and cultured on hormone-free MS medium, supplemented with a mixture of antibiotics and an antifungal. In order to study the hormonal requirements of the species, nodal segments were cultured on basal MS supplemented with...
Tipo: Journal article
Palavras-chave: In vitro; Micropropagation; Scrophulariaceae.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300017

In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.
Provedor de dados: 69
Autores: Piña-Escutia,José Luis; Vázquez-García,Luis Miguel; Arzate-Fernández,Amaury Martín.
Plants of Tigridia pavonia (L.f.) DC were regenerated from twin-scaling explants cultured on Murashige and Skoog and N6 basal medium. The highest formation of shoots per responding explant was obtained on N6 medium supplemented with 4.5 µM 2,4-dichlorophenoxy acetic acid in combination with 2.2 µM benzylaminopurine. Shoots rooted readily on N6 basal medium supplemented with 1 g l-1 activated charcoal and 2.6 µM naphtalenacetic acid. The rooted shoots achieved 100% survival. Inter Simple Sequence Repeat analysis was carried out to check for possible genetic alterations in plants obtained after two consecutive subcultures. The results revealed that the recovered plants did not exhibit any type of polymorphism.
Tipo: Journal article
Palavras-chave: Genetic integrity; Micropropagation; Oceloxóchitl.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100007

Wild ornamental germplasm exploration and domestication based on biotechnological approaches: In vitro colchicine treatment to obtain a new cultivar of Scoparia montevidiensis
Provedor de dados: 69
Autores: Escandón,Alejandro S; Miyajima,Ikuo; Alderete,Marisol; Hagiwara,Juan Carlos; Facciuto,Gabriela; Mata,Diego; Soto,Silvina M.
The genus Scoparia is native from Argentina. The diversity of colours and shape of their flowers bestows this genus a very interesting ornamental potential. The purpose of the present study is to explore the Scoparia species germplasm by means of in vitro polyploidization in order to improve their ornamental qualities. Accessions of S. montevidiensis var. montevidiensis, S. montevidiensis var. glandulifera, S. nudicaulis, S. hasleriana and S. dulcis were collected and maintained under greenhouse conditions. The Murashige-Skoog medium, supplemented with 0.25 mg/L BAP was used for the nodal segments multiplication of the five Scoparia species. Except for S. hasleriana, the multiplication rate of the other species ranged between 10 and 12 shoots per explant....
Tipo: Journal article
Palavras-chave: Chimera; Flow cytometry; Micropropagation; Ornamental plants; Tetraploid.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200010

In vitro establishment of pepper-rosmarin nodal segments
Provedor de dados: 75
Autores: Blank,Arie F; Costa,Andréa S da; Arrigoni-Blank,Maria de Fátima; Mendonça,Aline B de; Ledo,Ana da S.
Pepper-rosmarin (Lippia sidoides Cham.) is a native medicinal plant from the Brazilian biome Caatinga. Its high economical importance comes from the antimicrobial properties of thymol and carvacrol, both present in the pepper-rosmarin essential oil. Nodal segments of pepper-rosmarin were established in vitro to evaluate different growth regulators. We tested four concentrations of IBA (0.0; 0.01; 0.05; and 0.1 mg L-1) combined with six concentrations of BAP (0.0; 0.1; 0.5; 1.0; 2.0; and 4.0 mg L-1) in assay 1; five concentrations of NAA (0.0; 0.1; 0.25; 0.5; and 1.0 mg L-1) in assay 2; two concentrations of NAA (0.0 and 0.01 mg L-1) combined with five concentrations of kinetin (0.0; 0.25; 0.5; 1.0; and 2.0 mg L-1) in assay 3; five concentrations of IAA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lippia sidoides; Medicinal plant; Micropropagation; Growth regulator.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362008000200025

In vitro culture of jambu with different growth regulators
Provedor de dados: 75
Autores: Almeida,Susan P; Souza,Joanne MM; Amorim,Andredy MT; Gusmão,Sérgio AL de; Souza,Rodrigo ORM; Santos,Alberdan S.
ABSTRACT The aim of this study was to establish the best concentrations of growth regulators for in vitro cultivation of jambu for a subsequent elaboration of an efficient micropropagation protocol. After sterilized, the seeds were inoculated on different media (MS, ½MS and water-agar) for in vitro germination. Nodal segments of in vitro germinated jambu seedlings were used as explants in the micropropagation with different concentrations of 6-benzylaminopurine (BAP) (0.0; 0.125; 0.25; 0.50 and 0.75 mg L-1) and callus induction with 2.4- dichlorophenoxyacetic acid (2.4-D) (0.0; 0.25; 0.50; 0.75 and 1.0 mg L-1) on Murashige &amp; Skoog’s (MS) medium. The highest germination rates were obtained on MS medium with better seedling development and greater height...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acmella oleracea; Micropropagation; Callus induction; BAP; 2.4-D; Nodal segments.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000200134

Physiological development of zygotic embryos of heliconias propagated in vitro and conventionally
Provedor de dados: 75
Autores: Ulisses,Cláudia; Morais,Marciana; Barbosa,Marta R; Albuquerque,Cynthia C; Willadino,Lilia; Camara,Terezinha R.
ABSTRACT The aim of this study is to compare physiological development of Heliconia bihai cv. Lobster Claw Two plants derived from in vitro culture of zygotic embryos and conventional propagation. Heliconias obtained from rhizomes and from in vitro multiplication were evaluated every 30 days during ten months under greenhouse conditions. The experimental design was completely randomized, with ten repetitions, and the 2x10 factorial arrangement consisted of two plant multiplication methods and ten evaluations performed at different times. The analyzed biometric parameters were plant height, number of leaves, number of tillers, leaf area, and color intensity in the bracts. Plants derived from in vitro culture showed significant differences in the development...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heliconia bihai; Micropropagation; Rhizome; Vegetative propagation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000200229

Produção de material vegetativo de videira livre de virus no submédio São Francisco.
Provedor de dados: 14
Autores: LEAO, P. C. de S.; MELO, N. F. de; PIRES, E. da L.
Tem como objetivo produzir material vegetativo livre de virus das principais cultivares de videira de interesse comercial para o submedio Sao Francisco, proporcionando aos produtores e viveiristas da Regiao Nordeste do Brasil a possibilidade de disporem de mudas certificadas sadias e de elevada qualidade, evitando a disseminacao de viroses e, com isto, permitindo a implantacao de vinhedos homogeneos e de elevada produtividade.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Videira; Submedio Sao Francisco; Grape; Diseases; Resistance.; Uva; Biotecnologia; Doença; Muda; Meristema; Micropropagação; Resistência; Vírus.; Biotechnology; Meristems; Micropropagation; Seedlings.
Ano: 1997
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/133465

Protocolo de micropropagação para as espécies Piper hispidinervum e P. aduncum.
Provedor de dados: 14
Autores: RAPOSO, A.; TEIXEIRA, R. B.; MANFIO, C. E.; SILVA, D. A. da; MOREIRA, N. R. da S.; MACIEL, S. de A.
Este trabalho apresenta o protocolo de cultivo in vitro para as espécies Piper hispidinervum e P. aduncum, utilizando material oriundo de mudas obtidas por meio da germinação in vitro de sementes.
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Técnicas de laboratorio.; Cultivo in vitro; Micropropagación; Pimenta de macaco.; Micropropagação; Cultura in vitro; Pimenta longa; Piper hispidinervum; Piper longum; Micropropagation; In vitro culture; Laboratory techniques.; Piper aduncum.
Ano: 2012
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/943350

Efeito de reguladores de crescimento na multiplicação in vitro de brotos de abacaxizeiro.
Provedor de dados: 14
Autores: LEMOS, O. F. de; SILVA, S. P. G. da; ALBIM, E. de M. e S.; LAMEIRA, O. A.; REGO, J. C.; MENEZES, I. C. de.
bitstream/item/57407/1/CPATU-BP204.pdf
Tipo: Séries anteriores (INFOTECA-E)
Palavras-chave: Cabeça de onça; Belém; Pará; Brasil; Growth rate.; Abacaxi; Ananás Comosus; Micropropagação; Regulador de Crescimento.; Amazonia; Micropropagation; Pineapples; Varieties.
Ano: 1998
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/374240

Jardins in vitro : tecnologia e criatividade em pequenos recipientes.
Provedor de dados: 14
bitstream/item/77595/1/Folder-Jardins-in-vitro-2012.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Ornamental Grass.; Micropropagação; Reprodução vegetal; Planta ornamental; Micropropagation; Plant reproduction.
Ano: 2012
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/951625

Ensaios preliminares para o estabelecimento de um protocolo de assepsia, visando a micropropagação de cultivares de mangueira.
Provedor de dados: 14
Autores: CORDEIRO, M. C. R.; CID, L. P. B.; PINTO, A. C. de Q.; GOMES, A. C.; ANDRADE, S. R. M. de; VARGAS RAMOS, V. H.
RESUMO: Visando ao estabelecimento de um protocolo de assepsia para a micropropagação de diferentes cultivares de mangueira, foram coletadas no campo, por um período de dois anos (2000-2001), microestacas e folhas jovens e tratadas no laboratório com hipoclorito de sódio (v/v) 2% com Tween 80 a 0,025% por 15 minutos no caso de microestacas e, 0,5; 1,0; 1,5 e 2% com Tween 80 a 0,06 por 5 minutos no caso de folhas jovens. As microestacas, depois da assepsia com hipoclorito de sódio foram tratadas também com Benomyl 200mg/L por 30 minutos, ficando, em seguida, em magentas com vermiculita umedecidas em água estéril. As folhas jovens depois da assepsia foram colocadas em placas de Petri, contendo meio nutritivo básico SP com polivinilpirrolidona, Benomyl e...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Mango; Desinfectants.; Cultura de Tecido; Desinfecção; Explante; Manga; Mangifera Indica; Micropropagação; Reprodução Vegetal.; Explants; Plant propagation; Micropropagation; Tissue culture.
Ano: 2002
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/555088

Micropropagação da mandioca mediante ápices caulinares e segmentos nodais.
Provedor de dados: 14
Autores: SOUZA, A. da S.; SOUZA, F. V. D.; SANTOS-SEREJO, J. A. dos; JUNGANHS, T. G.; SILVA NETO, H. P. da.
Em plantios de mandioca é frequente observar-se, no momento da colheita, um número inferior de plantas em relação ao de manivas plantadas, devido à não brotação de manivas ou à morte de plantas. Ocorre uma notória desuniformidade no desenvolvimento das plantas, variação considerável na produção por planta e frequentes podridões das raízes, fenômeno este que ocasiona baixo rendimento. Geralmente, acredita-se que as causas desses problemas são de ordem climática e/ou edáfica; entretanto, a qualidade das manivas é outro fator muito importante.
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Mandioca; Micropropagação; Reprodução vegetal; Cassava; Micropropagation; Plant propagation.
Ano: 2008
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/655629

Cultura in vitro de embriões zigóticos de cupuaçuzeiro.
Provedor de dados: 14
Autores: LEDO, A. da S.; LAMEIRA, O. A.; MOURA, E.; MENEZES, I. C. de.
bitstream/item/42519/1/Boletim-Pesquisa-28.pdf
Tipo: Séries anteriores (INFOTECA-E)
Palavras-chave: Cupuaçuzeiro; Belém; Pará; Brasil.; Cultura de Tecido; Cultura In Vitro; Micropropagação; Theobroma Grandiflorum.; Amazonia; In vitro culture; Micropropagation; Tissue culture.
Ano: 2000
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/382729

Preliminar assay to establish a micropropagation protocol for mango (Mangifera indica, L.) cultivated in brazilian savannah.
Provedor de dados: 14
Autores: CORDEIRO, M. C. R.; BARRUETO, C. P. L.; PITO, A. C. Q.; GOMES, A. C.; ANDRADE, S. R. M.; VARGAS RAMOS, V. H.
bitstream/CPAC-2009/24171/1/p2002_60.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Tropical fruits.; Explante; Fruta Tropical; Manga; Mangifera Indica; Micropropagação; Melhoramento Vegetal; Propagação Vegetativa.; Explants; Plant propagation.; Micropropagation; Plant breeding.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567480

Desenvolvimento e avaliação de protocolos para descontaminação de explantes de mangueira visando à micropropagação.
Provedor de dados: 14
Autores: ANDRADE, S. R. M. de; PINTO, A. C. de Q.; FALEIRO, F. G.; CORDEIRO, M. C. R.; RAMOS, V. H. V.; TEIXEIRA, J. B.
RESUMO: A eliminação de microrganismos é uma condição essencial para o estabelecimento de um processo de regeneração in vitro, pois eles além de competirem pelos nutrientes também produzem toxinas que causam a morte do explante. O trabalho visou desenvolver metodologias de controle de crescimento de microrganismos provenientes de explantes de mangueira inoculados em meio de cultura, como fase inicial do estabelecimento de um protocolo de micropropagação de gemas laterais de mangueira para apoiar o programa de melhoramento desenvolvido na Embrapa. Contudo, uma bactéria de cor esbranquiçada, gram positiva, demonstrou ser resistente a todos os antibióticos, menos ao complexo sulfametaxazol + trimetroprima. Foi verificada a presença de outra bactéria, de...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Mango; Desinfection.; Cultura de Tecido; Desinfecção; Explante; Genética Vegetal; Manga; Mangifera Indica; Organogénese.; Genetics; Explants; Micropropagation; Tissue culture.
Ano: 2005
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/570345

Embriogênese somática e regeneração de plantas em açaizeiro.
Provedor de dados: 14
Autores: LEDO, A. da S.; LAMEIRA, O. A.; MENEZES, I. C. de.
O objetivo do presente trabalho foi estudar as diferentes respostas morfogenéticas de embriões zigóticos de açaizeiro (Euterpe oleracea Mart.) submetidos a várias condições de cultura in vitro. Os experimentos foram conduzidos em laboratório com material vegetal coletado de plantas de açaí na Embrapa Amazônia Oriental, Belém, PA, Brasil. Verificou-se a expressão de um modelo de embriogênese somática, direto, repetitivo a assincronizado em embriões zigóticos maduros cultivados em meio primário MS suplementado com 339.36 ? M de 2,4-diclorofenoxiacético (2,4-D) e transferidos para meio secundário MS na presença de 0,537 ? M de ácido 1-naftalenoacético (ANA) e 12,30 ? M de 2-isopenteniladenina (2iP). A conversão de embriões somáticos em plântulas foi alcançada...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Açaí de touceira; Embrapa Amazônia Oriental; Belém (PA); Pará; Eastern Amazon; Amazônia Oriental; Micropropagación.; Cultivo in vitro; Fitomejoramiento; Embriogénesis; Melhoramento genético vegetal; Açaí; Euterpe oleracea; Cultura in vitro; Embriogênese.; Micropropagação; Embryogenesis.; Micropropagation; Plant breeding; In vitro culture.
Ano: 2002
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/496030

Cultura de tecidos: (manual).
Provedor de dados: 14
Autores: LAMEIRA, O. A.; LEMOS, O. F.; MENEZES, I. C. de; PINTO, J. E. B. P.
bitstream/item/63281/1/Oriental-Doc66.pdf
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Manual.; Cultura de Tecido; Micropropagação.; Micropropagation; Tissue culture.
Ano: 2000
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/382772

Produção de mudas de teca por micropropagação.
Provedor de dados: 14
Autores: RAPOSO, A.; FERMINO JUNIOR, P. C. P.; TEIXEIRA, R. B.; PEREIRA, J. E. S.
A propagação in vitro de teca tem sido realizada por meio da cultura de tecidos vegetais de diferentes genótipos. Nesse contexto, o uso da micropropagação a partir de plantações de teca no Brasil pode ser uma importante ferramenta, pois permite a produção de grandes quantidades de novas plantas em um curto espaço de tempo, a partir de um único propágulo vegetativo retirado de uma planta matriz. Assim, o objetivo deste trabalho é descrever protocolos de micropropagação da espécie Tectona grandis a partir de genótipos estabelecidos na Amazônia Sul-Ocidental, no Estado do Acre.
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Teak; Producción de plántulas.; Cultivo in vitro; Madera tropical; Micropropagación; Tectona grandis; Muda; Produção.; Cultura in vitro; Essência florestal; Teca; Micropropagação; Tropical wood; Seedling production.; In vitro culture; Micropropagation.
Ano: 2010
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/872550

Automacao e racionalizacao na micropropagacao industrial.
Provedor de dados: 14
Autores: FARI, M.; MELO, N. F. de.
Tendencias da mecanizacao na propagacao in vitro em larga escala, automacao das etapas dos metodos tradicionais de cultura de tecidos, novastecnologias baseadas na utilizacao de sistemas de propagacao em meio liquido, desenvolvimento de sistemas de cultivo in vitro alternativos, sistema CLONER: uma nova iniciativa de racionalizacao da bancada de micropropagacao.
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Propagacao in vitro; In vitro propagation; Biotecnologia; Cultura de Tecido; Meristema; Micropropagação; Biotechnology; Micropropagation; Tissue culture.
Ano: 1996
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/131977

Somatic embryogenesis and in vitro plant regeneration from pejibaye adult plant leaf primordia
Provedor de dados: 86
Autores: Almeida,Marcílio de; Almeida,Cristina Vieira de.
The aim of this work was to evaluate a protocol for plant regeneration by means of somatic embryos obtained from isolated adult pejibaye leaf primordia, and to describe histological origin of embryos and morphogenetic response. Explants were cultivated in modified MS medium. Mesophyll parenchymatous cells originated meristemoids (preembryonic complex formation) induced with 7.1 µM BAP in the first two subculture periods. After polarized structures with 12.9 µM NAA and 3.55 µM BAP were formed in the third subculture, somatic embryos developed and regenerated normal plants. The mesophyll parenchymatous cells display high capacity of direct response to the auxin and cytokinin.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bactris gasipaes; Histology; Micropropagation; Peach palm.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000900015

Acclimatization of Tapeinochilos ananassae plantlets in association with arbuscular mycorrhizal fungi
Provedor de dados: 86
Autores: Oliveira,João Ricardo Gonçalves de; Morais,Thiago Alberto de Lima; Melo,Natoniel Franklin de; Yano‑Melo,Adriana Mayumi.
The objective of this work was to assess the potential of three isolates of arbuscular mycorrhizal fungi to promote growth of micropropagated plantlets of Tapeinochilos ananassae during acclimatization. The experiment was carried out in greenhouse, in a completely randomized block design, with four inoculation treatments: non‑inoculated control and plants inoculated with Glomus etunicatum, Acaulospora longula or Gigaspora albida, with ten replicates. After 90 days, the following parameters were evaluated: survival rate, height, leaf and tiller number, leaf area, fresh and dry biomass, contents of macro‑ and micronutrients in the root and shoot, glomerospore number, and mycorrhizal colonization. The survival percentage was 100%, except for plants inoculated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glomeromycota; Growth promotion; Micropropagation; Mineral nutrition; Tropical flowers.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000900018

Somatic embryogenesis and plant regeneration in elite clones of Theobroma cacao
Provedor de dados: 86
Autores: Silva,Thiago Édson Ribeiro da; Cidade,Luciana Cardoso; Alvim,Fátima Cerqueira; Cascardo,Júlio Cézar de Mattos; Costa,Marcio Gilberto Cardoso.
The objective of this work was to evaluated a procedure for somatic embryogenesis and regeneration of cacao (Theobroma cacao L.) elite clones. Petal explants from cacao clones TSH 565 and TSH 1188 were cultured on PCG and SCG-2 media, for calli growth. Somatic embryos were formed on the surface of embryogenic calli after transfer to embryo development (ED) medium. Clone TSH 565 showed a higher embryogenic potential than TSH 1188. The best combination of carbon source for embryo induction in ED medium was genotype-specific. Embryogenic callus formations increased in micropore tape-sealed Petri dishes, irrespective of cacao genotype. Mature somatic embryos were successfully converted into plantlets.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotechnology; Cacao; Gas exchange; Micropropagation; Morphogenesis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001000024

ANATOMICAL STUDIES OF IN VITRO ORGANOGENESIS INDUCED IN LEAF-DERIVED EXPLANTS OF PASSIONFRUIT
Provedor de dados: 86
Autores: GLORIA,BEATRIZ APPEZZATO DA; VIEIRA,MARIA LUCIA CARNEIRO; DORNELAS,MARCELO CARNIER.
With the aim of studying the organogenesis in vitro in Passiflora edulis Sims f. flavicarpa Deg., the passionfruit, leaf-derived explants were cultured on media containing NAA or BAP and incubated either in continuous darkness or in light. The histological events leading to de novo organ formation were evaluated. Darkness induces rhizogenesis in the presence of NAA, whereas direct shoot regeneration is stimulated by light and BAP. This latter condition is recommended for passionfruit micropropagation as several adventitious shoot buds were formed from meristemoids of parenchymal origin.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passiflora; Tissue culture; Micropropagation; Histology.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X1999001100005

In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions
Provedor de dados: 86
Autores: Silva,Tatiane Loureiro da; Scherwinski-Pereira,Jonny Everson.
The objective of this work was to evaluate in vitro storage of Piper aduncum and P. hispidinervum under slow-growth conditions. Shoots were stored at low temperatures (10, 20 and 25°C), and the culture medium was supplemented with osmotic agents (sucrose and mannitol - at 1, 2 and 3%) and abiscisic acid - ABA (0, 0.5, 1.0, 2.0 and 3.0 mg L-1). After six-months of storage, shoots were evaluated for survival and regrowth. Low temperature at 20ºC was effective for the in vitro conservation of P. aduncum and P. hispidinervum shoots. In vitro cultures maintained at 20ºC on MS medium showed 100% survival with slow-growth shoots. The presence of mannitol or ABA, in the culture medium, negatively affected shoot growth, which is evidenced by the low rate of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ex situ conservation; Germplasm bank; Micropropagation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000400007

Rescue of a non-viable accession and rapd analysis of recovered plants of Arachis retusa
Provedor de dados: 86
Autores: Gagliardi,Rachel Fatima; Pacheco,Georgia Pereira; Oliveira,Carlos Alberto; Carneiro,Leonardo Alves; Valls,José Francisco Montenegro; Vieira,Maria Lucia Carneiro; Mansur,Elisabeth.
In vitro regeneration of Arachis retusa was examined for the purpose of germplasm renewal and conservation. Random amplified polymorphic DNA (RAPD) fingerprinting was used to evaluate the genetic stability of plants derived from embryo axes and apical segments. Ten arbitrary decamer primers were screened and five of them were selected. Ninety genomic regions were evaluated, with an average of 18 loci per clone. All amplified segments were monomorphic. The results indicate that recovered plants are genetically stable at the assessed genomic regions and that both regeneration processes are suitable for in vitro germplasm preservation of Arachis species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Germplasm conservation; Micropropagation; Molecular characterization; Genetic stability.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000200015

Silicon and agar on in vitro development of cockscomb (Amaranthaceae)
Provedor de dados: 86
Autores: Assis,Franscinely Aparecida de; Souza,Genaina Aparecida de; Dias,Gabrielen de Maria Gomes; Assis,Gleice Aparecida de; Rodrigues,Filipe Almendagna; Pasqual,Moacir; Costa,Bárbara Nogueira Souza; Carvalho,Fabio Janoni.
Abstract: The objective of this work was to evaluate the effect of silicon and agar concentrations on cockscomb (Celosia cristata) (Amaranthaceae) development in vitro. Phytotechnical, anatomical, and ultrastructural analyses were carried out in a completely randomized design, in a 4x3 factorial arrangment, with four silicic acid concentrations (0.0, 0.5, 1.0, and 1.5 g L-1) and three agar concentrations (5.5, 8.0, and 10.5 g L-1), totaling 12 treatments with seven replicates. Celosia cristata explants were inoculated in vitro in a Murashige &amp; Skoog (MS) culture medium, with 30 g L-1 sucrose. The analyses were conducted 30 days after inoculation. Phytotechnical traits improve with agar concentration of 8.0 g L-1, and the association of this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Celosia cristata; Gelling agent; Micropropagation; Ornamental plant; Silicic acid.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000100030

Short-term storage in vitro and large-scale propagation of grapevine genotypes
Provedor de dados: 86
Autores: Silva,Rafael de Carvalho; Luis,Zanderluce Gomes; Scherwinski-Pereira,Jonny Everson.
The objective of this work was to evaluate the large-scale propagation of grapevine genotypes after short-term storage in vitro. Microshoots from ten grapevine genotypes were used. The following storage temperatures were evaluated: 10, 20, and 25°C. After short-term storage, the shoots were propagated in up to five successive subcultures, to assess the large-scale propagation of the germplasm maintained under conditions of minimal growth. The propagated shoots were rooted in different concentrations of indolbutiric acid (IBA) and acclimatized in greenhouse. The best temperature for short-term storage in vitro and survival of the genotypes was 20°C. In the propagation phase, the highest number of shoots per explant was found in the subcultures 4 and 5, with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitis; Germplasm; Ex situ conservation; In vitro conservation; Micropropagation; Minimal growth.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000300005

Influence of substrates and in vitro preconditioning treatments on ex vitro acclimatization of Arachis retusa
Provedor de dados: 86
Autores: Pacheco,Georgia; Gagliardi,Rachel Fátima; Cogliatti,Mariana Buturini; Manhães,Harlen Barreira; Carneiro,Leonardo Alves; Valls,José Francisco Montenegro; Mansur,Elisabeth.
The objective of this work was to evaluate the influence of substrate and preconditioning treatments on the acclimatization of in vitro plants of Arachis retusa. Plants were transferred to Plantmax or sand, and fertilized with Hoagland's nutrient solution. Plants maintained in sand, with or without fertilizer, showed the highest survival rates. In order to evaluate the influence of in vitro preconditioning treatments, stem segments were cultured on MS medium supplemented with different sucrose concentrations. The highest survival and developmental rates were observed in plants from two accessions cultured on MS supplemented with 1.5% and 3% sucrose. Flowering and fruit production were observed after five months.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arachis; Groundnut; In vitro conservation; Micropropagation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000100023

Solidifying agents and activated charcoal for in vitro culture of Solanum sessiliflorum
Provedor de dados: 86
Autores: Rodrigues,Filipe Almendagna; Rezende,Renata Alves Lara Silva; Pasqual,Moacir; Lopes,Maria Teresa Gomes.
Abstract: The objective of this work was to evaluate the effects of the solidifying agents agar and phytagel and of activated charcoal on the in vitro cultivation of two maná cubiu (Solanum sessiliflorum) varieties: Thaís and Santa Luzia. The phytotechnical characteristics analyzed included number of leaves, number of roots, shoot and root length, and fresh matter of shoot and root. Regardless of the variety, phytagel was superior to agar as a culture medium. A greater number of leaves and longer shoots were observed in the Santa Luzia variety, in the absence of charcoal. The Thaís variety showed longer shoots and roots in the presence of charcoal.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maná cubiu; Micropropagation; Plant tissue culture; Phytagel; Solanaceae.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001101123

Genetic and resistance stability to Black Sigatoka disease during micropopagation of Musa CIEN BTA-03 somaclonal variant
Provedor de dados: 85
Autores: Giménez,C; de García,E; Haddad,O.
Evaluation of clonal micropropagation and phenotype stability of elite somaclones are critical steps for development of new varieties. In the present work somaclon variant CIEN BTA-03 (resistant to Black Sigatoka), obtained through in vitro process from cultivar Williams (susceptible to Black Sigatoka), was micropropagated via apical shoot culture for five multiplication cycles in 0.5 mg/l of benzyl-aminopurine (BA). To verify the genetic stability of the progeny of this elite material, random amplified polymorphic DNA (RAPD) markers were used. A total of 5,292 monomorphic bands were obtained from the amplification of fifty six DNA samples (extracted from vitroplants randomly selected) with 10 different primer combinations. Non-polymorphic RAPD bands were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Genetic stability; RAPD; Disease resistance; Youngest leaf spotted (YLS).
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572008000100006

An efficient protocol for culturing meristems of sorghum hybrids
Provedor de dados: 85
Autores: Sadia¹,B; Josekutty,PC; Potlakayala,SD; Patel,P; Goldman,S; Rudrabhatla,SV.
A robust protocol for culturing meristems of Sorghum is required to assist with rapid genetic improvement of the genus. Through meristem culture, an efficient method for rapid micropropagation was developed for Sorghum bicolor (L.) Moench. hybrids, namely NC+262, NC+6C21 and NC+6B50. Complete plants were regenerated directly from shoot meristems without an intervening callus phase. Regeneration frequencies varied between the studied genotypes and according to the growth regulator combinations present in the medium. The combination of BAP and TDZ showed a synergistic effect on shoot multiplication. The highest number of shoots per meristem (68 ± 2) was recorded for the genotype NC+262, followed by NC+6C21 (31.3 ± 0.8), and NC+6B50 (16 ± 1.7). MS medium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Sorghum bicolor; Tissue culture; Genetic improvement; Genotypes.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000200010

Somatic embryogenesis in goiabeira serrana: genotype response, auxinic shock and synthetic seeds
Provedor de dados: 93
Autores: GUERRA,MIGUEL P.; DAL VESCO,LIRIO L.; DUCROQUET,JEAN PIERRE H.J.; NODARI,RUBENS O.; REIS,MAURÍCIO S. DOS.
The induction and control of in vitro somatic embryogenesis is dependent of a number of factors. The genotype of the mother plant donor of explants and the kind of auxin play essential roles in conferring embryogenic competence. Once obtained somatic embryos may be encapsulated in alginate originating synthetic seeds that can be stored at low temperatures. Considering this the present work aimed at the assessment of five different genotypes, five periods of 2,4-D shock (20 muM for 1, 2, 4, 8 and 16 weeks) (), and different substances supplemented to sodium alginate to obtain synthetic seeds. Zygotic embryos of Feijoa sellowiana were inoculated in basal LP medium (von Arnold and Erikson, 1981) supplemented with Morel’s vitamins, sucrose (3%) and phytagel...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acca sellowiana; Micropropagation; Pineapple-guava; Encapsulation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000200001

Agronomic characteristics of 'Prata-anã' and 'Maçã' bananas micropropagated
Provedor de dados: 94
Autores: Scandelai,Luís Henrique Mariano; Leonel,Sarita; Apponi,Luciene Michelle.
The experiment evaluated the agronomic characteristics of 'Prata-anã' and 'Maçã' banana plants, in the São Manuel region of São Paulo state. In the first crop cycle, the number of days from planting to inflorescence and from inflorescence to harvest; the number of shoots until the appearance of inflorescence and during the harvest period; active leaves number at inflorescence appearance and during the harvest period. Were evaluated yield parameters: like average weight of bunch, hand, rachis and fruit; average fruit number per hand and bunch; average fruit diameter and length. Incidences of plague diseases, as well as their severity were also evaluated. Delineation was totally at random, with 2 treatments, 15 replications and 5 useful plants per...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Banana; Musa spp; Micropropagation; Cycle.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452006000100041

Morphological identification of Ilyonectria liriodendri and its interaction with plant growth-promoting bacteria in grapevine rootstocks
Provedor de dados: 94
Autores: Brito,Liliane Martins de; Zanette,Flávio; Biasi,Luiz Antonio; Costa,Murilo Dalla; Passos,João Frederico Mangrich dos.
Abstract  In order to analyze the pathogenicity and antagonism between grapevine rootstocks and soil microorganisms, plant growth-promoting bacteria with antibiotic effect were selected from the phytopathogen Ilyonectria liriodendri, 2T-VSC-101 isolated monosporic, in in vitro antibiosis tests. The bacterial access that produced the largest zone of inhibition was used in the seedling biotization of the grapevine rootstocks P1103, IBBT481 and VR039-16. From the interaction between isolates of plant growth-promoting bacteria and Ilyonectria liriodendri isolate resulted in three bacterial isolates with control of the mycelial growth of the phytopathogen, all of the Burkholderia gender.However, the highest control bacterium, Burkholderia catarinensis sp.nov....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Acclimatization; Burkholderia catarinensis sp. nov.; Ilyonectria liriodendri.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000400401

Somatic embryogenesis from zygotic embryos of Euterpe oleracea Mart.
Provedor de dados: 94
Autores: Ledo,Ana da Silva; Lameira,Osmar Alves; Benbadis,Abdellatif Kemaleddine; Menezes,Ilmarina Campos de; Oliveira,Maria do Socorro Padilha de; Medeiros Filho,Sebastião.
The aim of this work was to study the morphogenetic responses of zygotic embryos of açai palm (Euterpe oleracea Mart.) submitted to several conditions of in vitro culture. Several research experiments were conducted, in laboratory, using vegetable material collected from açai palm plants at Embrapa Amazon Oriental, Belém-PA, Brazil. It was possible to verify the expression of a direct, repetitive and no-synchronized model of somatic embryogenesis in mature zygotic embryos cultivated in primary MS medium supplemented with 2,4-D (339.36 muM) and transferred to a secondary MS medium in the presence of NAA (0.537 muM) and 2iP (12.30 muM). The conversion of somatic embryos into seedlings was reached after 210 days with the transfer of the cultures to a third...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Açai palm; Growth regulators; Micropropagation; Tissue culture.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452002000300004

GROWTH OF ‘PRATA-ANA’ BANANA’S MICROSHOOTS CLONE GORUTUBA FROM SYNTHETIC SEEDS:SUBSTRATES AND BAP CONCENTRATION
Provedor de dados: 94
Autores: PEREIRA,JÚLIO CÉSAR GOMES; ROCHA,SELMA SILVA; LONDE,LUCIANA CARDOSO NOGUEIRA; MOTA,MARCELA CAROLINE BATISTA DA; ALVES,PABLO FERNANDO SANTOS; VIANA,WANDER SILVA.
ABSTRACT  The banana crop stands out as an activity of great social and economic importance in Brazil, which occupies the fifth place in world production. Synthetic seed production is becoming promising for a micropropagation and in vitro conservation. The aim of the study was to analyze the conversion and growth of ‘Prata-anã’ banana’s microshoots clone Gorutuba from synthetic seed in MS medium and vermiculite, different substrates and concentrations of BAP (6-benzylaminopurine) associated with ANA (acetic naphthalene acid) in the constitution of its capsule were tested. The microshoots were immersed in the sodium alginate matrix (3%) and dripped in a solution of CaCl2.2H2O (100 mM) for complexation and then in KNO3 solution (100 mM) to decomplex. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Encapsulation; Micropropagation; Musa sp.; Growth regulators.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000500704

Biotechnology applied to Annona species: a review
Provedor de dados: 94
Autores: Encina,Carlos Lopez; Martin,Elisabeth Carmona; Lopez,Antonio Arana; Padilla,Isabel Maria Gonzalez.
Annonaceae is an ancient family of plants including approximately 50 genera growing worldwide in a quite restricted area with specific agroclimatic requirements. Only few species of this family has been cultivated and exploited commercially and most of them belonging to the genus Annona such as A. muricata, A. squamosa, the hybrid A. cherimola x A. squamosa and specially Annona cherimola: the cherimoya, commercially cultivated in Spain, Chile, California, Florida, México, Australia, Ecuador, Peru, Brazil, New Zealand and several countries in South and Central America. The cherimoya shows a high degree of heterozygosis, and to obtain homogeneous and productive orchards it is necessary to avoid the propagation by seeds of this species. Additionally, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Annona; In vitro; Micropropagation; Protoplasts; Organogenesis; Polyploidy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452014000500002

Temporary immersion biorreators: efficient technique for the propagation of the ‘Pircinque’ strawberry
Provedor de dados: 94
Autores: Camargo,Samila Silva; Rufato,Leo; Magro,Maicon; Souza,André Luiz Kulkamp de.
Abstract  The in vitro propagation technique via temporary immersion bioreactors is a tool that, through the culture in a liquid medium, allows an increase in the efficiency of seedling production. Several researches with the strawberry crop have shown greater efficiency of the system compared to the conventional process of micropropagation in solid medium. In this sense, the objective herein was to establish a protocol of multiplication and rooting of the ‘Pircinque’ strawberry, in temporary immersion bioreactors. Two distinct and independent studies were carried out, characterized by the multiplication and rooting stages of strawberry explants, newly introduced and registered in Brazil. Two culture media (MS and KNOP) were studied and, as a control...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fragaria Ananassa; Micropropagation; Automation; Liquid medium.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000101001

Rooting and acclimatization of the Japanese plum tree, cv. América
Provedor de dados: 94
Autores: Bandeira,Juliana de Magalhães; Thurow,Liane Bahr; Braga,Eugenia Jacira Bolacel; Peters,José Antonio; Bianchi,Valmor João.
Rooting and acclimatization are limiting steps in plant micropropagation, especially in woody plant species. This study aimed to evaluate the IAA and IBA effect on the in vitro rooting and acclimatization of micropropagated shoots of Japanese plum (Prunus salicina Lindl.) cv. América. Shoots from 3 to 4 cm long were inoculated in MS medium with half salt and vitamin concentrations (MS/2) added with IAA and IBA (0, 0.25, 0.5, 0.75 and 1 mg L-1). After a 20-day period in in vitro cultivation, the shoots were evaluated, and then transferred to a greenhouse, and evaluated after 30 days. At the end of the in vitro cultivation period, no significant interactions were observed for number of roots per shoot and rooting percentage, but a significant effect was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prunus salicina; Micropropagation; Auxins.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452012000200035

Pineapple (Ananas comosus) cv. pérola ex vitro growth and mycorrhizal colonization affected by in vitro sucrose concentration
Provedor de dados: 94
Autores: Kunze,Alceu; Lovato,Paulo Emílio; Costa,Murilo Dalla; Dal Vesco,Lírio Luiz.
The aim of this study was to evaluate the effect of sucrose concentration in the culture medium on growth and on the establishment of mycorrhizas during the acclimatization of pineapple cv. Pérola. The plantlets were micropropagated in MS culture medium with 0, 10, 20 and 30 g L-1 of sucrose and then they were acclimatized during 12 weeks under greenhouse conditions, in a sandy soil - compost mixture, uninoculated or inoculated with a Rhizophagus clarus isolate. Plantlets from the culture medium with 20 g and 30 g of sucrose L-1 showed higher shoot and root biomass than those from sugar-free medium. Mycorrhizal colonization was lower in plantlets micropropagated in sucrose-free medium, but the intensity of arbuscules did not differ among treatments. In the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhizophagus clarus; Micropropagation; Weaning; Arbuscular mycorrhiza.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452014000300032

Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants
Provedor de dados: 94
Autores: Souza,Fernanda Vidigal Duarte; Canto,Ana Maria Mascarenhas Eloy; Souza,Antônio da Silva; Costa,Maria Angélica Pereira de Carvalho.
This work aimed to evaluate the influence of naphthaleneacetic acid (NAA) and gibberellic acid (GA3) plant regulators in in vitro etiolation and subsequent regeneration of the PE x SC-60 pineapple hybrid. Nodal segments of in vitro plants with approximately 5-7 cm height were incubated in basic MS culture medium supplemented with 0.0; 0.5 and 1.0 mg L-1 of naphthaleneacetic acid (NAA) in combination with gibberellic acid (GA3) in concentrations of 0.0; 0.5 and 1.0 mg L-1, and maintained at 27 ºC under dark condition. Evaluations were carried out at 90 and 180 days after incubation period. The best results for length of etiolated stems were obtained with 1.0 mg L-1 of NAA. In the experiment followed by the regeneration, stems with 3 cm from the etiolation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ananas comosus var. comosus; In vitro culture; Plant regulators; Nodal segments; Germplasm conservation; Micropropagation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452010000200035

In vitro propagation and acclimatization of Lippia rotundifolia, an endemic species of Brazilian Campos Rupestres
Provedor de dados: 124
Autores: Resende,Cristiano Ferrara de; Bianchetti,Ricardo Ernesto; Oliveira,Aline Mystica Silva de; Braga,Virgínia Fernandes; Peixoto,Paulo Henrique Pereira.
ABSTRACTThe importance in folk medicine, combined to threats in their environment, becomes necessary to carry out studies involving large-scale propagation of Lippia genus. Although the tissue culture propagation is widely disseminated for medicinal plants, for L. rotundifolia any article was published yet. The present study aimed to establish an efficient protocol for micropropagation of L. rotundifolia. Nodal segments, taken from plants collected in the Espinhaço Range, were disinfected, and cultures were initiated on MS medium with PVPP (1 g L- 1), sucrose (3%) and agar (0.7%). The culture were maintained in a growth room at controlled conditions. Disinfestation procedures and the supply of PVPP on culture media resulted in both reduced contamination...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; In vitro multiplication; In vitro rooting; Ex vitro acclimatization; Biodiversity conservation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902015000300582

Standardized production of Phyllanthus tenellus Roxb. by plant tissue culture
Provedor de dados: 124
Autores: Victório,Cristiane Pimentel; Henriques,Anaize Borges; Tavares,Eliana Schwartz; Esquibel,Maria Apparecida; Lage,Celso Luiz Salgueiro.
Exigencies as ethic plant raw material are part of the needs of modern phytotherapy. Micropropagation offers opportunities to obtain mass propagation of superior genotypes in short time. This study aimed to develop a protocol of direct and indirect organogenesis of Phyllanthus tenellus Roxb. Nodal segments from plantlets obtained by in vitro germination were subcultured in modified Murashige and Skoog medium added with different plant growth regulators: IAA (indole-3-acetic acid), IBA (indole-3-butyric acid), GA3 (3-giberelic acid) and KIN (kinetin). The highest proliferation rate was obtained using the combinations: IBA, KIN + GA3 (3.5 mg L-1) and IBA + KIN (2.4 mg L-1). Rooting was intensified after 40 days, reaching 100% for all media with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Callus; Micropropagation; Medicinal plant.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902010000200015

Acclimatization of 'VR043-43' (Vitis vinifera x Vitis rotundifolia) grapevine rootstock
Provedor de dados: 63
Autores: Machado,Marília Pereira; Biasi,Luiz Antonio; Ritter,Marlice; Ribas,Luciana Lopes Fortes; Zanette,Flávio.
The pre-acclimatization stage can be used to improve micropropagation protocols and increase the yield of produced plants. The influence of sucrose and photon flux density (PFD) levels on the acclimatization of in vitro-grown 'VR043-43' (Vitis vinifera x Vitis rotundifolia) grapevine rootstocks was evaluated. Rooted shoots were obtained from 4-week-old in vitro shoots cultivated in QL (Quoirin and Lepoivre, 1977) culture medium supplemented with 15, 30 and 45 g L-1 of sucrose. The experiment was kept in a 25 ± 2ºC growth room, under 16-h photoperiod and PFD of 18 µmol m-2 s-1 or 43 µmol m-2 s-1. Plants were transferred to an intermittent misting system greenhouse for 10 d followed by 20 d of once-a-day watering routine using a handheld hose. Plant height...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue culture; Sucrose; Micropropagation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000400007

Organogenesis and transient genetic transformation of the hybrid Eucalyptus grandis × Eucalyptus urophylla
Provedor de dados: 63
Autores: Alcantara,Giovana Bomfim de; Bespalhok Filho,João Carlos; Quoirin,Marguerite.
The hybrid Eucalyptus grandis x Eucalyptus urophylla presents high levels of productivity and potential for use in paper, cellulose and fiber industries. The bud organogenesis from leaf explants of two clones of E. grandis × E. urophylla was studied in order to verify the effect of several factors: subculture duration on multiplication medium, type of explants, entire and half leaves: basal and apical portions, and duration of the culture on a regeneration medium. Differences in organogenic capacity of the two clones tested were observed. The explant most recommended for organogenesis is the basal section of the leaf collected from shoot clusters subcultured every 17 days. Moreover, the leaf explants must be transferred to a fresh bud induction medium...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Agrobacterium tumefaciens; Micropropagation; Tissue culture; Transgenic plant; Woody species.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000200016

Slow-grown in vitro conservation of Heliconia champneiana cv. Splash under different light spectra
Provedor de dados: 63
Autores: Rodrigues,Paulo Hercílio Viegas; Arruda,Flávia; Forti,Victor Augusto.
ABSTRACT: In vitro techniques, with the purpose of conserving the genetic resources of plants, are fundamental to the feasibility of establishing germplasm banks and enabling the commercial production of micropropagated plants. The aim of this study was to evaluate the use of different spectra of light in in vitro germplasm conservation by slow grown storage of Heliconia Champneiana cv. Splash. Explants of heliconia in vitro were submitted to the following light treatments: CW (control white), B100 (100 % blue), R100 (100 % red) and R70B30 (70 % red/30 % blue), all with PFD = 25 μmol m2 s–1, maintained in vitro for two time periods, namely P1, 6 weeks and P2, twelve weeks, and were statistically evaluated after each interval with respect to: height, fresh...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue culture; Micropropagation; Ornamental plants.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000200163

Tissue culture of ornamental cacti
Provedor de dados: 63
Autores: Pérez-Molphe-Balch,Eugenio; Santos-Díaz,María del Socorro; Ramírez-Malagón,Rafael; Ochoa-Alejo,Neftalí.
Cacti species are plants that are well adapted to growing in arid and semiarid regions where the main problem is water availability. Cacti have developed a series of adaptations to cope with water scarcity, such as reduced leaf surface via morphological modifications including spines, cereous cuticles, extended root systems and stem tissue modifications to increase water storage, and crassulacean acid metabolism to reduce transpiration and water loss. Furthermore, seeds of these plants very often exhibit dormancy, a phenomenon that helps to prevent germination when the availability of water is reduced. In general, cactus species exhibit a low growth rate that makes their rapid propagation difficult. Cacti are much appreciated as ornamental plants due to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cactaceae; Areole activation; Conservation; Micropropagation; Ornamental cactus.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000600540

IN VITRO GERMINATION AND VEGETATIVE PROPAGATION THROUGH BUD DEVELOPMENT OF SACHA INCHI (Plukenetia volubilis)
Provedor de dados: 37
Autores: HENAO RAMÍREZ,Ana María; URREA TRUJILLO,Aura Inés; ATEHORTÚA GARCÉS,Lucía.
ABSTRACT This study describes the in vitro seed germination and micropropagation of Plukenetia volubilis (sacha inchi), an oilseed crop rich in omega-3 fatty acids, with health benefits and several industrial applications. Seed germination was evaluated in different culture media (MS and 1/2 MS), seed coat presence/absence and culture temperature (18 °C and 28 °C). Micropropagation was performed using axillary bud development (ABD) on nodal segments from in vitro seedlings. KIN, BAP and 2-ip were evaluated for ABD, and the effect of modified MS in 453 mg L-1 CaCl2 and 351.62 mg L-1 MgSO4 on ABD and shoot survival was assessed to improve the process. Finally, six treatments were evaluated to optimize ABD and shoot leaf formation. Seed germination of 91.6 %...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Axillary buds; Calcium; Kinetin; Micropropagation; 2-ip.
Ano: 2022
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2022000100070

An efficient system for in vitro propagation of Bouchea fluminensis (Vell.) Mold. (Verbenaceae)
Provedor de dados: 39
Autores: Resende,Cristiano Ferrara de; Braga,Virgínia Fernandes; Silva,Cristiane Jovelina da; Pereira,Paula da Fonseca; Ribeiro,Cleberson; Salimena,Fátima Regina Gonçalves; Peixoto,Paulo Henrique Pereira.
This study aimed to establish and propagate in vitro plants of Bouchea fluminensis, a medicinal species known in Brazil as gervão-falso ("false verbena"), evaluating the influences of different growth regulators on in vitro multiplication and rooting stages, as well as examining ex vitro acclimatization of rooted plants. Explants were established on Murashige and Skoog medium at half strength of salts and vitamins without growth regulators. For multiplication, the explants were subjected to combinations of 6-benzyladenine (BA; 0, 2.5, 5.0 and 7.5 µM) and α-naphthalene-acetic acid (NAA; 0, 0.2, 0.4 and 0.6 µM). The medium found to induce the greatest number of shoot was that containing 5 µM of BA (NAA-free). For rooting, we evaluated three auxins (NAA,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Biodiversity conservation; Espinhaço Mountain Range.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000200005

Survival and development of reintroduced Cattleya intermedia plants related to abiotic factors and herbivory at the edge and in the interior of a forest fragment in South Brazil
Provedor de dados: 39
Autores: Endres Júnior,Delio; Sasamori,Márcio Hisayuki; Schmitt,Jairo Lizandro; Droste,Annette.
ABSTRACT Biotic and abiotic factors, such as luminosity, temperature, air humidity, and herbivory, can affect the establishment of reintroduced plants in natural habitats. This study evaluated the effects of these factors on the survival and growth of Cattleya intermedia plants reintroduced into a forest fragment in South Brazil. Plants of C. intermedia were obtained from in vitro seed germination in asymbiotic culture. Eighty-eight plants were reintroduced at both the forest edge and forest interior. Plants with greater shoot heights and number of leaves and pseudobulbs suffered more damage from herbivores at the edge. There were no significant differences in morphometric parameters between damaged and non-damaged plants in the interior. Tenthecoris...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Micropropagation; Monitoring; Orchidaceae; Reintroduction.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000400555

In Vitro Propagation of Heliconia bihai (L.) L. from Zygotic Embryos
Provedor de dados: 39
Autores: Ulisses,Cláudia; Melo-de-Pinna,Gladys Flávia; Willadino,Lilia; Albuquerque,Cynthia Cavalcanti de; Camara,Terezinha Rangel.
The internal morphology of embryos from immature and mature fruits of Heliconia bihai (L.) L. cv. Lobster Claw Two was examined. Embryos were inoculated into MS media (full MS and ½ MS) and GA3 (0, 2.5 and 5 mg L-1) with either sucrose or glucose. These plantlets were then replicated and transferred to MS medium (full MS or ½ MS) with 0 or 2.5 mg L-1 BAP and their multiplication was evaluated 30 and 45 days after inoculation. The genetic variability of the multiplied plants was estimated using isoenzyme analyses. The internal morphology of the mature embryos revealed their tissues to be in more advanced stages of differentiation than immature embryos. In the conversion phase, 85% of the inoculated embryos developed into plants in the ½ MS medium with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carbohydrates; Embryo culture; Histology; Isoenzymes; Micropropagation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062010000100018

In vitro propagation of Anathallis adenochila (Loefgr.) F. Barros (Orchidaceae), a species endemic to southern and southeastern Brazil
Provedor de dados: 39
Autores: Endres Júnior,Delio; Sasamori,Márcio Hisayuki; Droste,Annette.
Anathallis adenochila is a small epiphytic orchid, endemic to the Atlantic Forest in the southern and southeastern regions of Brazil. Information on the species is scarce and limited to distribution and occurrence data, with no reports about its development. In the present study, plantlets were propagated in vitro, and the influence of different concentrations of macronutrient salts and sucrose on the survival and development of the species was assessed. The analysis were performed on complete Murashige and Skoog (MS) medium and half MS medium (with half-strength macronutrients) containing 10, 30 or 60 g L-1 of sucrose. Plantlet survival, height of the aerial part, and the length of the longest root were significantly greater on half MS medium containing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Carbon source; Micropropagation; Orchid; Mineral salts.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000400001

Resposta rizogênica in vitro de ápices caulinares de mamoeiro ‘Tainung 01’ em diferentes tempos de permanência em meios de indução e regeneração - DOI: 10.4025/actasciagron.v31i4.863
Provedor de dados: 1
Autores: Schmildt, Edílson Romais; UFES; Amaral, José Augusto Teixeira do; UFES; Schmildt, Omar; UENF; Coelho, Ruimário Inácio; UFES; Rabello, Wanderson Souza; UENF; Martins Filho, Sebastião; UFV.
Este trabalho teve como objetivo avaliar a resposta rizogênica in vitro de ápices caulinares de plantas jovens de mamoeiro ‘Tainung 01’, em diferentes tempos de permanência em meios de indução e regeneração. O meio de indução empregado foi o MS, suplementado com complementos orgânicos constituídos de sacarose, mio-inositol, tiamina, piridoxina, ácido nicotínico, além de AIB. O meio de regeneração diferiu do meio de indução por ser desprovido de AIB. Os tratamentos consistiram em tempos variáveis de permanência dos explantes nos meios de indução e de regeneração (indução/regeneração): 0/25; 5/20; 10/15; 15/10; 20/5 e 25/0 dias. O delineamento experimental foi inteiramente casualisado, com seis tratamentos e quatro repetições, e cada unidade experimental foi...
Palavras-chave: 5.01.03.04-0    Produção de Mudas Carica papaya; Auxina; Micropropagação; Cultura de tecidos Carica papaya; Auxin; Micropropagation; Tissue culture.
Ano: 2009
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/863

Activated charcoal and graphite for the micropropagation of Cattleya bicolor Lindl. and a orchid double-hybrid ‘BLC Pastoral Innocence’ - doi:  10.4025/actasciagron.v34i2.12257
Provedor de dados: 1
Autores: Prizão, Eliane Cristina; Universidade Estadual de Maringá; Gonçalves, Letícia de Menezes; Universidade Estadual de Maringá; Gutierre, Maria Auxiliadora; Universidade Estadual de Maringá; Mangolin, Claudete Aparecida; Universidade Estadual de Maringá; Machado, Maria de Fátima Pires da Silva; Universidade Estadual de Maringá.
No previous studies have investigated the use of graphite instead of activated charcoal in orchids. In this work, different concentrations of activated charcoal or graphite were added to KC medium to darken the culture medium and stimulate the in vitro propagation of Cattleya bicolor and of a double hybrid orchid (‘BLC Pastoral Innocence’). The seedlings were inoculated on growth regulator-free KC medium; the effects of activated charcoal (0.0, 1.5, 3.0, 4.5, 6.0, and 7.5 g L-1) and graphite (0.0, 1.5, 3.0, 4.5, 6.0, and 7.5 g L-1) concentrations were tested separately. Each flask contained fifteen seedlings, and the experiments had a randomised, two-way factorial design. The two tested variables were culture medium and illumination levels; eleven culture...
Tipo: Laboratory analysis
Palavras-chave: Activated charcoal; Graphite; Orchids; Micropropagation.
Ano: 2012
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12257

Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279
Provedor de dados: 1
Autores: Kavand, Somayeh; Agricultural Biotechnology Research Institute of Iran; Kermani, Maryam Jafarkhani; Agricultural Biotechnology Research Institute of Iran; Haghnazari, Ali; Agricultural Biotechnology Research Institute of Iran; Khosravi, Pegah; Agricultural Biotechnology Research Institute of Iran; Azimi, Mohamad Reza; Agricultural Biotechnology Research Institute of Iran.
In Iran, a large number of Rosa species have been exposed to extinction and therefore preservation techniques are necessary to safeguard their future. In the present investigation, the objectives were to optimize the micropropagation and medium-term conservation of one of these wild species, Rosa pulverulenta. At proliferation stage, the maximum number of new leaves (9.6) were produced on the medium containing 4 &amp;micro;M BAP + 0 &amp;micro;M GA3, whereas the maximum number of axillary shoots (4.1) were observed in the medium containing 4 &amp;micro;M BAP + 3 &amp;micro;M GA3. The results for rooting experiments suggested that the highest increase in stem height (49.5 mm) at the acclimatization stage was observed in plantlets treated with 1 &amp;micro;M...
Tipo: Laboratory analysis
Palavras-chave: In vitro conservation; Micropropagation; Proliferation; Recovery; Rooting; Rosa pulverulenta in vitro conservation; Micropropagation; Proliferation; Recovery; Rooting; Rosa pulverulenta.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279

Desenvolvimento de protocolo para microenxertia do tomateiro Lycopersicon esculentum Mill - DOI: 10.4025/actasciagron.v32i1.968
Provedor de dados: 1
Autores: Coutinho, Ozimar de Lima; UFRR; Rego, Maílson Monteiro do; UFPB; Rego, Elizanilda Ramalho do; UFPB; Kitamura, Mario Couquiti; UFRR; Marques, Luciano Façanha; UFPB; Farias Filho, Leonildo de Paula; UFRR.
A murcha bacteriana, causada por Ralstonia solanacearum, é a principal doença vascular de plantas em todo o mundo. O agente causal é uma bactéria que ocorre em todas as regiões do Brasil, predominando em condições de umidade e temperatura elevadas, fatores propícios ao desenvolvimento do patógeno. Este agente causal é de difícil controle, pois sobrevive nos mais diversos hospedeiros, especialmente Solanaceas, além de outras famílias de importância alimentar, condimentar e medicinal. O uso de espécies do gênero Solanum na enxertia convencional e minienxertia em tomateiro Lycopersicon esculentum é limitada em função da incompatibilidade. Neste trabalho, utilizou-se o método da microenxertia para obtenção de plantas resistentes a doenças como a murcha...
Palavras-chave: 5.01.00.00-9    Agronomia Solanaceae; Murcha bacteriana; Ralstonia solanacearum; Micropropagação; Cultura in vitro 5.01.03.05-9; Solanaceae; Bacterial wilt; Ralstonia solanacearum; Micropropagation; In vitro  culture.
Ano: 2010
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/968

Níveis de ácido indol butírico (AIB) no enraizamento in vitro de microestacas de mamoeiro ‘Tainung 01’ - DOI: 10.4025/actasciagron.v32i1.866
Provedor de dados: 1
Autores: Schmildt, Edílson Romais; UFES; Amaral, José Augusto Teixeira do; UFES; Schmildt, Omar; UENF; Coelho, Ruimário Inácio; UFES; Rabello, Wanderson Souza; UENF; Martins Filho, Sebastião; UFV.
Este trabalho teve como objetivo avaliar níveis de AIB no enraizamento in vitro de microestacas de mamoeiro (Carica papaya L. ‘Tainung 01’). Ápices caulinares de plantas com 120 dias de idade foram estabelecidos e avaliados em meio MS, suplementado com complementos orgânicos constituídos de sacarose a 87,64 mM, mio-inositol a 555,06 µM, tiamina-HCl a 0,30 µM, piridoxina-HCl a 2,43 µM, ácido nicotínico a 4,06 µM e 0; 0,74; 1,48; 2,96 e 5,92 µM de AIB, em delineamento inteiramente casualizado, com quatro repetições e cinco tubos de ensaio por parcela. Após 30 dias, avaliou-se a percentagem de enraizamento, o crescimento das microestacas, o comprimento da maior folha e a massa de calo. Observou-se que o maior percentual estimado de enraizamento ocorreu com a...
Palavras-chave: 5.01.00.00-9    Agronomia Carica papaya; Auxina; Micropropagação; Cultura de tecidos Fitotecnia Carica papaya; Auxin; Micropropagation; Tissue culture.
Ano: 2010
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/866

Morpho-anatomical characterization of embryogenic calluses from immature zygotic embryo of peach palm during somatic embryogenesis - DOI: 10.4025/actasciagron.v32i2.3248
Provedor de dados: 1
Autores: Maciel, Simone Alencar; Universidade Federal do Acre; Fermino Junior, Paulo Cesar Poeta; Universidade Federal do Acre; Silva, Ricardo Alexandre; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Scherwinski-Pereira, Jonny Everson; Embrapa Recursos Genéticos e Biotecnologia.
The objective of this study was to morpho-anatomically characterize nodular embryogenic calluses from zygotic embryos of peach palm during the induction of somatic embryogenesis. Immature zygotic embryos were pre-treated in MS medium added to Picloram and 2,4-D (25 &amp;micro;M) and BAP (0, 5, 10 &amp;micro;M). After three months, primary calluses were transferred to MS induction medium added to Picloram and 2,4-D (450 &amp;micro;M). After six months, the embryogenic calluses were then histologically analyzed and cultivated in the maturation medium. The competent tissues of the zygotic embryos differentiated embryogenic calluses under action of both Picloram and 2,4-D auxins (450 &amp;micro;M), where the presence of multi-granular structures were observed....
Palavras-chave: Bactris gasipaes; Micropropagation; Histology; Morphology; Callus anatomy; Somatic embryos 5.00.00.00-4 Bactris gasipaes; Micropropagation; Histology; Morphology; Callus anatomy; Somatic embryos Ciências Agrárias.
Ano: 2010
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3248

Dynamics of proteins, carbohydrates and global DNA methylation patterns during induction of nodular cluster cultures from seeds of Vriesea reitzii
Provedor de dados: 1
Autores: Corredor, Jenny; De Conti, Daniela; Guerra, Miguel Pedro; Dal Vesco, Lirio Luiz; Pescador, Rosete.
Tissue culture techniques have been employed for bromeliad mass propagation by means of the morphogenetic route of nodular cluster cultures (NCs). This study aimed to assess protein, carbohydrate and global DNA methylation (GDM) level dynamics during NCs induction from Vriesea reitzii seeds. Seeds were inoculated into Murashige and Skoog (MS) liquid medium supplemented with 4 µM α-naphthaleneacetic acid (NAA) to induce NCs and in culture medium without plant growth regulators to form normal seedlings. Samples collected at 0, 3, 7, 10, 14, and 21 days of culture were analyzed. All parameters assessed showed the same variation pattern. However, seeds inducing NCs showed significantly lower starch (6.0 mg g-1 FM), carbohydrate (10.7 mg g-1 FM) and GDM (11.0%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bromeliaceae; Bromeliad; Germination; Micropropagation; Seedling. Bromeliaceae bromeliad germination micropropagation seedling.
Ano: 2020
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42448

Biochemical characterization of systemic bacteria in bananas, sensitivity to antibiotics and plant phytotoxicity during shoot proliferation
Provedor de dados: 1
Autores: Oliveira, Janiffe Peres de; Scherwinski-Pereira, Jonny Everson.
The objective of this work was to characterize the biochemically systemic bacterial isolated from banana plants, to evaluate the bacterial sensitivity to antibiotics, and to determine the phytotoxicity of banana shoots during in vitro proliferation. Systemic bacteria belonging to the Klebsiella and Aeromonas genera were isolated from the “Maravilha” (FHIA 01 AAAB), “Preciosa” (PV 4285 AAAB) and “Thap Maeo” (AAB) varieties and were then characterized. Tests of shoot sensitivity to antibiotics were performed, and the minimum inhibitory concentration (MIC) and phytotoxic effects of selected antibiotics to plants were determined. Among the 20 antibiotics evaluated, the strains showed sensitivity to cefaclor, cefalexin, cefalotin, nalidixic acid,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ciências agrárias Musa spp.; Micropropagação; Contaminação; Controle microbiano. Produção de mudas Musa spp.; Micropropagation; Contamination; Endophytic microorganisms; Antimicrobial control.
Ano: 2016
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/27689

Estabelecimento de protocolo para multiplicação in vitro de Bastão-do-imperador (Etlingera elatior) Jack RM Sm. - doi: 10.4025/actasciagron.v32i4.4830
Provedor de dados: 1
Autores: Colombo, Larissa Abgariani; UEL; Assis, Adriane Marinho de; UEL; Faria, Ricardo Tadeu de; UEL; Roberto, Sergio Ruffo; UEL.
O objetivo deste trabalho foi estabelecer um protocolo para a multiplicação in vitro de Bastão-do-imperador (Etlingera elatior). Brotos axilares foram inoculados em meio MS contendo as seguintes concentrações de reguladores de crescimento durante a fase de isolamento: testemunha; BAP 2,25 mg L-1; BAP 2,25 mg L-1 + ANA 0,93 mgL-1; BAP 2,25 mg L-1 + AIA 0,87 mg L-1; BAP 4,95 mg L-1; BAP 4,95 mg L-1 + ANA 0,93 mg L-1; BAP 4,95 mg L-1 + AIA 0,87 mg L-1. As brotações provenientes desta fase foram subcultivadas em um novo meio de cultura MS: testemunha; BAP 2,25 mg L-1; BAP 3,37 mg L-1; BAP 4,50 mg L-1; BAP 2,25 mg L-1 + ANA 1,12 mg L-1; BAP 3,37 mg L-1 + ANA 2,25 mg L-1; BAP 4,50 mg L-1 + ANA 3,37 mg L-1 e avaliou-se a taxa de multiplicação dos brotos por...
Palavras-chave: 5.01.03.04-0 Zingiberaceae; Micropropagação; Reguladores de crescimento Produção de mudas Zingiberaceae; Micropropagation; Plant growth regulators.
Ano: 2010
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/4830

Effects of glutathione supplementation and carbon source during somatic embryogenesis of Acca sellowiana (O.Berg) Burret (Myrtaceae)
Provedor de dados: 3
Autores: Pavei, Ana Flávia; Fraga, Hugo Pacheco de Freitas; Vieira, Leila do Nascimento; Guerra, Miguel Pedro.
 The present study intended to investigate the effects of different glutathione (GSH) levels (0, 0.1, 0.5 and 1 mM) on the somatic embryogenesis (SE) induction of Acca sellowiana. Besides, we evaluated the effect of different carbon sources (sucrose and maltose) on the somatic embryos conversion. GSH-supplemented treatments resulted in improved SE induction rates (~70%) as compared to the control GSH-free (~35%) after 50 days of culture. The total number of somatic embryos obtained did not differ between treatments, but significant differences were observed for the embryonic stages after 80 days of culture. After 80 days of culture, 0.5 and 1 mM GSH-supplemented treatments showed the largest amount of torpedo-staged somatic embryos. In contrast, treatments...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2.03.00.00-0 Botânica in vitro culture; Micropropagation; Somatic embryo conversion; Feijoa. 2.03.06.00-8 Botânica Aplicada.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/40257

Induction of in vitro shoots of Billbergia euphemiae E. Morren (Bromeliaceae) from leaf explants
Provedor de dados: 3
Autores: Simão, Mariela Justiniano; Faria, Daniele Vidal; Werner, Elias Terra; Soares, Taís Cristina Bastos; Gontijo, Andreia Barcelos Passos Lima.
Bromeliads are an important group for the maintenance of the Atlantic Forest, with many threatened species due to exacerbated extraction and destruction of their natural habitats. Considering the need of developing protocols for the conservation of these species, the aim of this work was to evaluate the effect of different growth regulators in the in vitro induction of shoots of Billbergia euphemiae. Leaf explants were excised from seedlings derived from in vitro germination and grown on MS medium supplemented with NAA (0, 1 or 2 μM) and BA (0, 2, 4 or 6 μM) combinations. The evaluation of the number of shoots per explant, shoot length, number of leaves per shoot and longest leaf length average was carried out after 30 and 60 days of culture. The best in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2.03.06.00-8 BA; Bromeliad; Micropropagation; NAA; Organogenesis Botânica Aplicada.
Ano: 2016
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31694

Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of Cattleya tigrina A. Richard
Provedor de dados: 3
Autores: Almeida, Vivian; Fraga, Hugo Pacheco de Freitas; Navarro, Bruno Bachiega; Guerra, Miguel Pedro; Pescador, Rosete.
Cattleya tigrina is endemic to the Atlantic forest biome and classified as vulnerable in the Red Book of Brazilian Flora. In vitro techniques comprise valuable tools for the conservation of endangered plant species. The aim of the present study was to evaluate the morphological features, global DNA methylation levels and free polyamines during protocorm-like bodies (PLBs) induction of C. tigrina. Along with that, an efficient protocol for in vitro propagation of this species is proposed. The first evidences of PLBs induction in C. tigrina occurred at seven days in culture, starting from the basal portion of the leaf abaxial surface. A hypomethylation marked the beginning of cell differentiation, followed by an increased global DNA methylation at 35 days in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2.03.00.00-0; 2.03.03.00-9; 2.03.06.00-8 orchids; Micropropagation; In vitro culture; Epigenetics; PLBs. Botânica.
Ano: 2017
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36656

Micropropagated banana plants induced by gamma irradiation and resistant to the root-knot nematode reproduction
Provedor de dados: 111
Autores: Taha, Rania Abdel-Ghaffar; El-Nagdi, Wafaa M. A.; Kamel, Hedaya Ahmed.
Micropropagation of banana plants has been used successfully in commerce. Banana plantation has a great interest in Egypt due to suitable climate. However, some problems might thwart the plantation progress like the root-knot nematode; Meloidogyne incognita infection which can affect acclimatization and plantation of in vitro plants. Thus, our investigation aimed to produce nematode resistant in vitro plants using gamma radiation (γ). Banana in vitro plantlets were exposed to gamma radiation at several doses then replanted in vitro. Sensitivity of banana plants to irradiation was determined. Acclimatized plants were infected with M. incognita in greenhouse to see their capability for tolerance. Results indicated that irradiated plants could manage the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Musa sp.; Micropropagation; Gamma radiation; Meloidogyne incognita; Free amino acids.
Ano: 2020
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/5500

Rooting and acclimatization of Apuleia leiocarpa plantlets
Provedor de dados: 115
Autores: Haygert-Lencina,Kelen; Antônio-Bisognin,Dilson; Kielse,Paula; Pimentel,Nathalia.
Abtract Apuleia leiocarpa is an endangered tree species with high quality timber. Propagation by seed is difficult due to irregular fruiting and water-impermeable tegument, so asexual propagation is a promising alternative. This study aimed to evaluate in vitro and ex vitro rooting of A. leiocarpa and acclimatization of micropropagated plantlets. The hypothesis was that type of explant, indolebutyric acid (IBA) concentration and substrate influence the induction of adventitious roots in apuleia. For in vitro rooting, nodal segments and micro-cuttings were grown in Wood Plant Medium (WPM) with 0, 4.9, 9.8, 14.7 and 19.6 μM IBA. Compositions of commercial substrate + vermiculite + coarse sand and commercial substrate + vermiculite, in equal proportions, were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apuleia leiocarpa; Indolebutyric acid; Micropropagation; Micro-cuttings; Substrate composition.
Ano: 2017
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952017000800909

Mini-incubators improve the adventitious rooting performance of Corymbia and Eucalyptus microcuttings according to the environment in which they are conditioned
Provedor de dados: 42
Autores: BRONDANI,GILVANO E.; OLIVEIRA,LEANDRO S. DE; KONZEN,ENÉAS R.; SILVA,ANDRÉ L.L. DA; COSTA,JEFFERSON L.
ABSTRACT We addressed a major challenge in the in vitro clonal propagation of Corymbia citriodora, Eucalyptus urophylla and E. benthamii by using an ex vitro adventitious rooting strategy in a mini-incubator. Mini-incubators were placed in four environments for rooting. A shade house with no fogging system and a greenhouse with no ventilation but with a fogging environment had the best performance in terms of rooting, root growth and survival of microcuttings. Daily recording of the temperature within each mini-incubator in each environment allowed the verification of negative correlations between the maximum average temperature and the survival, adventitious rooting and root growth. The ideal maximum air temperature for the efficient production of clonal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maximum average temperature; Micropropagation; Rhizogenesis; Shade house; Greenhouse.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502409

Morpho-physiological changes in Billbergia zebrina due to the use of silicates in vitro
Provedor de dados: 42
Autores: MARTINS,ADALVAN D.; MARTINS,JOÃO PAULO R.; BATISTA,LUCAS A.; DIAS,GABRIELEN M.G.; ALMEIDA,MIRIELLE O.; PASQUAL,MOACIR; SANTOS,HELOÍSA O. DOS.
Abstract The use of silicon in Billbergia zebrina cultivation in vitro is an alternative for optimizing micropropagation of this important ornamental plant species. The objective of the present study was to evaluate the growth and anatomical and physiological alterations in Billbergia zebrina (Herbert) Lindley plants as a function of different sources and concentrations of silicon during in vitro cultivation and acclimatization. The experimental design was completely randomized, with a double factorial arrangement and an additional control treatment (2 x 3 + 1). The first factor was relative to calcium silicate and sodium silicate added to the Murashige &amp; Skoog culture medium; the second factor was related to its concentrations, 0.5, 1.0, and 2.0 mg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bromeliad; Micropropagation; Plant anatomy; Plant physiology; Silicon.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703449

Organogenesis and plant regeneration of Arachis villosa Benth. (Leguminosae) through leaf culture
Provedor de dados: 47
Autores: Fontana,María Laura; Mroginski,Luis Amado; Rey,Hebe Yolanda.
With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: &amp;#945;-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 &amp;#956;M thidiazuron and 4.44 &amp;#956;M 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in Arachis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro cultivation; Leaflet explants; Micropropagation; Thidiazuron; Wild groundnut.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000300006

Genetic stability in rice micropropagation
Provedor de dados: 47
Autores: Medina,R; Faloci,M; Marassi,M.A.; Mroginski,L.A.
An efficient clonal propagation procedure for six rice varieties cultivated in Argentina was developed by using shoot tip cultures, and the genetic stability of the micropropagated plants was verified by isozyme analysis. One week old seedlings obtained on MS medium were sectioned and subcultured on MS medium (0.75% agar) supplemented with different combination and concentrations of cytokinins (BAP and KIN) and auxins (2,4-D and NAA). After four weeks of culture, multiple shoots were obtained. The best response was observed on MS supplemented with BAP 5 mg l-1. Shoot clumps were multiplied in MS liquid medium containing BAP 5 mg l-1. Profuse rooting was obtained after transfer to MS medium lacking growth regulators and with sucrose 8% (w/v). Complete...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Multiple shoots; Oryza sativa L.; Isozyme; Genetic stability.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000100002

In vitro propagation of Opuntia ellisiana Griff. and acclimatization to field conditions
Provedor de dados: 47
Autores: Juárez,María Cecilia; Passera,Carlos Bernardo.
The genus Opuntia is a valuable forage resource in arid and semiarid lands during periods of drought and shortage of herbaceous plants. However, absolute minimum temperatures in the plains of Mendoza represent a limiting factor to cultivate several species. Opuntia ellisiana is a cold hardy species, so the goals of this study were to massively propagate it using in vitro culture techniques, and then to acclimatize plantlets obtained to field conditions. Different sterilization protocols were tested. Areoles were isolated in laminar airflow cabinet, and cultured on Murashige-Skoog medium, supplemented with sucrose and different BAP and IBA combinations. Explants were grown at 27±2ºC, under a 16-h photoperiod. The shoots produced were used in the rooting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Areole culture; Cold hardy forage; Arid forage resources.
Ano: 2002
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452002000300002

Inhibitory effect of jasmonic acid and ethylene on epicotyl growth and bud induction in the maritime pine, Pinus pinaster Soland. in Ait
Provedor de dados: 47
Autores: Martin,Maria Teresa; Pedranzani,Hilda; García-Molinero,Patricia; Pando,Valentin; Sierra-de-Grado,Rosario.
Two independent parameters, epicotyl height (cm) and number of induced buds were studied on Pinus pinaster explants to analyse the effects of three phytohormones (6-benzylaminopurine, jasmonic acid, ethylene) which were combined or not in 11 different treatments. Epicotyle length diminished significantly in relation to the control medium (medium without exogen phytohormones) in presence of jasmonic acid, 6-benzylaminopurine or Ethephon (which is converted to ethylene in plants) in any of treatments. Concentrations of 100 &amp;#956;M of jasmonic acid and Ethephon had a greater inhibitory effect than the treatments with 10 &amp;#956;M. In addition to that, jasmonic acid was a stronger inhibitor than Ethephon in any of the tried combinations. There were no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Phytohormones; Plant growth regulators; 6-benzylaminopurine; In vitro plant culture.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000300002

Micropropagation of Ilex dumosa (Aquifoliaceae) from nodal segments in a tissue culture system
Provedor de dados: 47
Autores: Luna,C.; Sansberro,P.; Mroginski,L.; Tarragó,J.
Micropropagation of Ilex dumosa var. dumosa R. ("yerba señorita") from nodal segments containing one axillary bud was investigated. Shoot regeneration from explants of six-year-old plants was readily achieved in ¹/4 strength Murashige and Skoog medium (¹/4 MS) plus 30 gr·L-1 sucrose and supplemented with 4.4 mM BA. Further multiplication and elongation of the regenerated shoots were obtained by subculture in a fresh medium of similar composition with 1.5 gr·L-1 sucrose. Rooting induction from shoots were achieved in two steps: 1) 7 days in ¹/4 MS (30 gr·L-1 sucrose, 0.25 % Phytagel®) with 7.3 mM IBA and 2) 21 days in the same medium without IBA and 20 mM of cadaverine added. Regenerated plants were successfully transferred to soil. This micropropagation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Nodal Segment; Cytokinins; Ilex Dumosa.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000200006

Behavior and preservation of an in vitro collection of European aspen in Spain
Provedor de dados: 47
Autores: Martin,M.T.; Pedranzani,H.E.; Sierra de Grado,R.
An in vitro collection has been established with selected European aspen from Palencia province (Spain). Currently, this collection includes 32 high quality clones, selected for their good bearing and healthy state. Most of them belong to different discrete local populations. Populus tremula L. was propagated in proliferation Aspen Culture Medium; they required subculture every 3 months. The purpose of this study was, therefore, to select a medium which allows the maintenance of 32 clones for a period longer than 3 months without subculture and to observe the behavior of those clones in 15 different culture medium compositions. Seven nodal cutting stem explants from each clone were cultured in parallel in the different media. One and three months after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Populus tremula; Germplasm conservation; In vitro plant culture; Micropropagation; Selected clone.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100005

In vitro spore culture and reproductive aspects of the annual fern Anogramma chaerophylla (Pteridaceae)
Provedor de dados: 51
Autores: Luna,María L; Yañez,Agustina; Ramos Giacosa,Juan Pablo; Gorrer,Daniel; Berrueta,Pedro C; Giudice,Gabriela E.
Anogramma chaerophylla belongs to a fern genus with annual sporophytes and potentially perennating gametophytes. In the studied area, Natural Reserve Punta Lara, the plants grow stressed mainly by pollution and the invasion of exotic species. As a part of a project on conservation of ferns inhabiting Buenos Aires province, the objectives of this work were to evaluate in Anogramma chaerophylla the conditions for in-vitro spore germination and to analyse the different stages of its reproductive cycle. Spores were sterilized in an aqueous solution 10 % of NaCIO during different times and then sown in Petri dishes with Murashige &amp;amp; Skoog medium, without the addition of sucrose. The dishes were kept under laboratory conditions, at 12 h light/ darkness...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anogramma chaerophylla; Micropropagation; Gametophyte; Tubercle; Ex-situ conservation.
Ano: 2016
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722016000400010

Effect of 6-BA on nodal explant bud sproutings of Coffea arabica cv. Mundo Novo
Provedor de dados: 53
Autores: Ramos,Luis Carlos da Silva; Almeida,Julieta Andrea Silva de.
Coffee plants can be micropropagated by nodal bud sprouting using the 6-benzylaminopurine (6-BA) hormone. However, literature reports the use of a wide range of 6-BA, from 0.5 to 88.8 µM L-1. So, this study was performed to narrow that range. Nodal explants of Coffea arabica cv Mundo Novo obtained from in vitro plantlets were inoculated on gelled-MS medium supplemented with different concentrations of 6-BA. Two assays were carried out: in the first one, 6-BA was used at concentrations of 0, 5, 25, 50, and 100 µM L-1, being evaluated at 43 and 123 days. In the second experiment, dosis of 10, 20 and 30 µM L-1, have evaluated at 65 and 100 days. Treatments with 6-BA induced multiple sprouting from the nodal explants, which were best characterized around 100...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Coffee; Nodal bud; Micropropagation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052005000200003

Production of pre-basic potato seed by polyvinyl chloride PVC: articulate gutters hydroponic system
Provedor de dados: 52
Autores: Scherwinski-Pereira,Jonny Everson; Medeiros,Carlos Alberto Barbosa; Fortes,Gerson Renan de Luces; Pereira,Arione da Silva.
The development of more efficient and productive systems for pre-basic seed potato production would improve the quality of the propagative material used by the potato growers, directly affecting the crop yields. A two-year experiment was carried out to evaluate the potato pre-basic seed production by two types of hydroponic systems (fibrocement tiles and articulated PVC gutters), two cultivars (`Baronesa` and `Eliza`) and two types of propagative material (plants coming from in vitro culture and minitubers). The PVC gutters system was highly efficient. When using minitubers, this system reached multiplication rates up to 74 tubers per plant. Minitubers were more productive than in vitro plants, independent of cultivar and hydroponic system utilized.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum tuberosum; Soilless culture; Multiplication; Propagation; Minitubers; Micropropagation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500007

In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe
Provedor de dados: 52
Autores: Delmail,David; Labrousse,Pascal; Lesouëf,Jean-Yves; Le Hir,François; Autret,Jean-Luc; Botineau,Michel.
Normania triphylla is an endemic species from Madeira island (Portugal) extinct in the wild since 1991. The aim of this work was to culture the meristems of this species in vitro and to multiply its shoots in order to preserve this endangered species. The best results in terms of multiplication were obtained in Murashige and Skoog medium supplemented with 10 µM 6‑benzylaminopurine (BAP). The number of shoots, the number of nodes and the number of leaves were the most important in this medium. However, the best results concerning the total shoot length were obtained when BAP was not supplemented into the medium and in the presence of 5 or 7.5 µM 1-naphtalene acetic acid (NAA). This is the first report on the in vitro culture of N. triphylla which could...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Normania; Endemic species; Micropropagation; 6‑benzylaminopurine; Callus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400009

Induction of leafy galls in Acacia mearnsii De Wild seedlings infected by Rhodococcus fascians
Provedor de dados: 52
Autores: Quoirin,Marguerite; Bona,Cleusa; Souza,Elizandra F. de; Schwartsburd,Pedro B.
Plantlets of blackwattle (Acacia mearnsii De Wild) were inoculated with the bacterium Rhodococcus fascians and cultured in vitro. Leafy galls appeared at the cotyledonary nodes in 75% of the infected plants. The galls were separated from the plants and cultured on a medium containing three-quarters-strength MS salts (Murashige and Skoog, 1962), MS vitamins, 2% sucrose and an antibiotic (cephalothin), supplemented with or without 0.2% activated charcoal. Histological studies conducted from the sixth to the twenty-second day after plant infection revealed the presence of newly formed meristematic centers, first in the axillary region, then on the petioles and lamina of the leaflets around the apical meristem. Approximately 37% of the galls developed one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Black wattle; Legume tree; Micropropagation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300002

Plant regeneration through somatic embryogenesis of yacón [smallanthus sonchifolius (poepp. and endl.) H. Robinson]
Provedor de dados: 52
Autores: Corrêa,Cynthia Manyra; Oliveira,Graciele Nicolodi de; Astarita,Leandro Vieira; Santarém,Eliane Romanato.
Smallanthus sonchifolius has tuberous roots containing large amounts of fructo-oligosaccharides and its medicinal use has increased due to the hypoglycemic properties reported for this species. An efficient system for propagation via somatic embryogenesis is reported using petiole segments cultivated on MS medium supplemented with combinations of BA, kinetin and 2,4-D, under light and darkness conditions. Embryogenic callus was formed in most of the treatments; however, somatic embryogenesis was promoted by the presence of light. Clusters of somatic embryos appeared on callus surface after 50 days of culture. The highest number of embryos was produced on 0.45 µM BA and 4.5 µM 2,4-D. Embryogenic calli were maintained on MS medium containing 4.5 µM BA and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plant; Tissue culture; Polymnia sonchifolia; Micropropagation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300005

In vitro multiplication of 'Flordaguard' rootstock: cytokinin source and concentration effects, explants orientation and period of permanence in the culture medium
Provedor de dados: 52
Autores: Radmann,Elizete Beatriz; Bianchi,Valmor João; Fachinello,José Carlos; Ferreira,Letícia Vanni; Oliveira,Roberto Pedroso de.
This work aimed at studying the effects of different sources and cytokinin concentrations, as well as explants orientation and time of their permanence in shoots induction medium to obtain high multiplication rate. Four experiments were carried out to evaluate the sprout percentage mean, shoots number per explant, elongated shoots percentage and shoots length. The treatments with 2iP and zeatin did not promote in vitro plant multiplication. When different BAP concentrations were tested, an increase in sprout percentage was obtained using BAP concentration up to 3.66 mg L-1 (80%), and for shoots number per explant a linear behavior was observed, showing a mean of four shoots per explant with 4.0 mg L-1 BAP. For the 'Flordaguard' rootstocks, the explants...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Cytokinin; Explant; Prunus.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100004

Crude Fermented Extract Containing Gibberellic Acid Produced by Fusarium moniliforme is an Alternative to Cost Reduction in Biofactories
Provedor de dados: 52
Autores: Costa,Jefferson da Luz; Silva,André Luís Lopes da; Gollo,André Luiz; Brondani,Gilvano Ebling; Santos,Leandro Freire dos; Rodrigues,Cristine; Vandenberghe,Luciana Porto de Souza; Soccol,Carlos Ricardo.
ABSTRACT Nidularium procerum and Nidularium innocentii (Bromeliaceae) were cultivated in vitro on media supplemented with different sources and levels of GA3 (gibberellic acid). These sources were the commercial powder (analytical degree) and fermented extract obtained by Fusarium moniliforme via solid state fermentation. The in vitro elongation and rooting of these plants were evaluated after 50 days of cultivation. The GA3 present in the fermented extract possess the same effect of purified GA3 (analytical degree) for the increase of the height of aerial part of shoots of N. innocentii, but not for the N. procerum being the GA3 fermented extract in a lesser degree. The GA3 fermented extract influences negatively the rooting in N. innocentii, while GA3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shelf life; Bromeliad; Gibberellin; Solid state fermentation; Micropropagation; Biofactory.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100427

Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plant
Provedor de dados: 52
Autores: Sharmin,Shamima Akhtar; Alam,Md. Jahangir; Sheikh,Md. Mominul Islam; Sarker,Kanak Kanti; Khalekuzzaman,Muhammad; Haque,Md. Anwarul; Alam,Mohammad Firoz; Alam,Iftekhar.
In this work, plant regeneration via somatic embryogenesis was achieved from leaf and internode derived callus of Wedelia calendulacea, an endangered medicinal plant. Primary callus was induced by culturing leaf disc and internode explant on Murashige and Skoog medium supplemented with 2.0 mg L-1 of 2,4-D under light condition. Transfer of embryogenic callus on a reduced concentration of 2,4-D facilitated somatic embryo development while calluses remained unorganized at the same 2,4-D level. A histological analysis confirmed somatic embryo by revealing the presence of a closed vascular system in the developing embryos and lack of a vascularconnection with surrounding callus tissues. Somatic embryos germinated into plantlets upon transfer on MS medium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro; Medicinal plant; Micropropagation; Somatic embryo; Threatened; Wedelia calendulacea.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300013

In vitro Callus Induction and Plant Regeneration of Celosia argentea- An Important Medicinal Plant
Provedor de dados: 52
Autores: Bakar,Dalilah Abu; Ahmed,Bakrudeen Ali; Taha,Rosna Mat.
Celosia argentea (Var.) cristata (Amaranthaceae) is a widely cultivated ornamental plant, which has antibacterial, astringent, haemostatic, hypertensive, ophthalmic, and parasitic significance. This study describes a protocol for in vitro callus induction and plant regeneration from leaf and stem explants of C. argentea using Murashige and Skoog (MS) medium. Callus culture was initiated and established from seedling, leaf, and stem explants. Explants were cultured on MS medium supplemented with auxin alone (0.5 mg/L Naphthaleneacetic acid (NAA), 2, 4-Dicholorophenoxyacetic acid (2, 4-D). Green and red compact callus (98%) were induced using MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L Benzyladenine (BA). Two different concentrations (1.0 mg/L BA +...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Celosia argentea; Amaranthaceae; In vitro; Micropropagation; Callus; Plant regeneration.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600860

Micropropagation of an elite F1 watermelon (Citrullus lanatus) hybrid from the shoot tip of field grown plants
Provedor de dados: 52
Autores: Khalekuzzaman,Mohammad; Khatun,Marufa; Rashid,Mohammad Harunur; Sheikh,Mominul Islam; Sharmin,Shamima Akhtar; Alam,Iftekhar.
The aim of this work was to develop a protocol for rapid micropropagation of an elite F1 hybrid watermelon cultivar using shoot tip of field-grown plants. Maximum frequency (73%) of shoot tip showed growth response in MS medium supplemented with 5 mg l-1 benzyl adenine (BA) and 0.1 mg l-1 indole-3 acetic acid (IAA). Upon transfer to cytokinin-enriched medium, the cultures produced multiple shoots and 2.0 mg l-1 BA was optimum in this respect. Addition of gibberellic acid (GA3) in the multiplication medium resulted in better growth of shoots. Rooting rate was 100% when shoots were obtained from second subculture were cultured in medium with 1.0 mg l-1 indole-3 butyric acid (IBA). The shoots produced more roots with increasing number of subcultures. About...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro; Watermelon; Micropropagation; Hybrid vigor.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300002

Biotic Contamination and Possible Ways of Sterilization: A Review with Reference to Bamboo Micropropagation.
Provedor de dados: 52
Autores: Ray,Syandan Sinha; Ali,Nasim.
ABSTRACT Multipurpose use of bamboo in rural life makes it as poor man’s timber in Asian countries. Deforestation and industrialization leads to destruction of natural forest. To replenish, a rapid plantation of bamboo could be one of the possible solutions. Bamboo is propagated mainly by vegetative methods though it is not suitable for large scale plantation because of several limitations. Micropropagation is gaining importance for large scale propagation because of its capability in raising huge number of true to type propagules in a limited space in very short span of time. Like any other plant, the chief constraint of bamboo micropropagtion is in vitro contamination arises from several sources including explants. Most of the contaminants are reduced by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotic contamination; Sterilization; Bamboo; Micropropagation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100207

Assessment of Genetic Fidelity of in vitro Raised Plants in Swertia chirayita through ISSR, RAPD analysis and Peroxidase Profiling during Organogenesis
Provedor de dados: 52
Autores: Sharma,Vikas; Belwal,Nidhi; Kamal,Barkha; Dobriyal,Anoop Kumar; Jadon,Vikash Singh.
ABSTRACT An efficient in vitro regeneration protocol for medicinally important herb Swertia chirayita was developed and the genetic fidelity was assessed using RAPD and ISSR markers. The best shoot regeneration was observed on MS basal supplemented with 1.0 mg/L Benzyl amino purine (BAP) in combination with Indole-3-acetic acid (IAA) (0.5 mg/L) that resulted in the increase by multiplication rate (7.65) with an average of 33.33 numbers of shoots and average shoot length of 2.70 cm. It was further enhanced by the addition of adenine sulfate (0.007%) that resulted in an average of 42 shoots per clum with 4.13 cm of average shoot length and the increase in multiplication fold to 9.75 that further resulted in the reduced use of other cytokinins and auxins. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swertia chirayita; Micropropagation; Adenine sulfate; Ascorbate peroxidase; RAPD; ISSR.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100434

Antimicrobial action of the essential oil of Lippia gracilis Schauer
Provedor de dados: 52
Autores: Albuquerque,Cynthia Cavalcanti de; Camara,Terezinha Rangel; Mariano,Rosa de Lima Ramos; Willadino,Lilia; Marcelino Júnior,Cristiano; Ulisses,Cláudia.
Antimicrobial activity of the essential oil (EO) of Lippia gracilis Schauer over fungi and heliconia endophytic bacteria was evaluated. For the fungi Geotrichum candidum; Trichoderma viride; Torula herbarum; Paecillomyces sp.; Fusicoccum sp.; P. aeruginens; Curvularia lunata; Aspergillus nidulans; A. flavus; and A. niger mycelial discs were inoculated in PDA medium with oil (0; 420; 440; 460; 480 e 500 µL L-1). The inhibition percentage in 420 µL L-1 was 100% for all fungi, except for C. lunata and A. niger, which was 95.58% and 89.40% , respectively. In other experiment, a suspension of the Salmonella choleraceuis-diarizonae, Enterobacter asburiae, Bacillus thuringiensis, B. pumilus, B. cereus, Klebsiella pneumoniae, and E. hormaechei was individually...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Contamination; Monoterpenes.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500001

Factors affecting "in vitro" plant development and root colonization of sweet potato by Glomus etunicatum Becker &amp;amp; Gerd
Provedor de dados: 58
Autores: Bressan,Wellington.
Nutrients media (Murashige &amp;amp; Skoog, Hoagland &amp;amp; Arnon and White's media) supplemented or not with sucrose and substrates (vermiculite, agar and natrosol) were tested for their effects on plant development and root colonization of micropropagated sweet potato, cv. White Star, by Glomus etunicatum Becker &amp;amp; Gerdemann (isolate INVAM FL S329). Addition of sucrose (3%) did not affect plant development. However, hyphal growth and root colonization were depressed. Contrasting responses to media nutrient concentration were observed for plant height, root colonization, and hyphal growth. The highest concentration of nutrients in Murashige &amp;amp; Skoog medium improved plant development, but this medium decreased hypha growth and inhibited...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arbuscular mycorrhiza; Micropropagation; Media composition; Substrate.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100006

Multiple shoot formation in Hypericum perforatum L.and hypericin production
Provedor de dados: 61
Autores: Santarém,Eliane Romanato; Astarita,Leandro Vieira.
Hypericum perforatum is a traditional medicinal plant with wound healing and antidepressive properties. Among the secondary compounds of interest is hypericin, a naphtodianthrone that seems to participate in the medicinal effects of this species. The aim of this work was to obtain an efficient micropropagation system of H. perforatum and to compare the hypericin content between in vitro and field-grown plants. Cultures were initiated from nodal segments of mature plants inoculated onto MS medium supplemented with 4.5 muM BA, kinetin, thidiazuron, individually or in combination with 0.05 muM NAA. Organogenic explants were observed on medium with either BA or kinetin alone or in combination of these with NAA. Subculture of organogenic explants onto the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Medicinal plant; Micropropagation; Organogenesis; St. John's Wort.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000100006

Influence of Vessel Type, Physical State of Medium and Temporary Immersion on the Micropropagation of Three Rhodophiala species
Provedor de dados: 117
Autores: Muñoz,Manuel; Seemann,Peter; Jara,Gloria; Riegel,Ricardo.
Rhodophiala C. Presl (Amaryllidaceae) is a genus of attractive flowering geophytes native to South America. They have ornamental value, but most species are little known and have conservation problems. The objective of this study was to optimize a micropropagation process to support the use and preservation of three Chilean native species, R. montana (Phil.) Traub, R. splendens (Rengifo) Traub., and R. ananuca (Phil.) Traub. The research evaluated the feasibility of implementing liquid medium culture and assessed the influence of different tissue culture systems on the shoot production and biomass increment of small bulbs. Three experiments were carried out. The first one determined the influences of flask size and volume of media; the second compared...
Tipo: Journal article
Palavras-chave: TIS; Liquid culture; Micropropagation; Acclimatization.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000400014

Plant tissue culture: Current status, opportunities and challenges
Provedor de dados: 118
Autores: García-Gonzáles,Rolando; Quiroz,Karla; Carrasco,Basilio; Caligari,Peter.
In the last two decades plant biotechnology applications have been widely developed and incorporated into the agricultural systems of many countries worldwide. Tissue culture tools have been a key factor to support such outcomes. Current results have allowed plant biotechnology and its products -including transgenic plants with several traits- to be the most assimilated technology for farmers and companies, representing several benefits such as: 125 millions ha of transgenic crops in 2008, the reduction of pesticides application by up to 9% in the last ten years, transgenic plants with a better nutritional quality, mass propagation of selected and healthy plants, and the production of proteins for industrial or therapeutic use. The rapid and extensive...
Tipo: Journal article
Palavras-chave: In vitro; Micropropagation; Organogenesis; Plant bioreactors; Somatic embryogenesis; Transgenic plants.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000300001

Micropropagation of Stevia rebaudiana plants
Provedor de dados: 65
Autores: Rokosa,Marta Teresa; Kulpa,Danuta.
ABSTRACT:  The aim of the study was to develop optimum composition of plant growth regulators in media for the propagation and rooting of shoots of stevia (Stevia rebaudiana Bertoni) in in vitro cultures. Single-node shoot fragments obtained from plants propagated on MS medium were placed onto media supplemented with: BAP, 2iP and KIN at concentrations: 0.5, 1, 2 and 5 mg∙dm-3, whereas at the rooting stage with addition of: IAA, IBA and NAA at concentrations 1, 2, 4 and 8 mg∙dm-3. The highest number of shoots and leaves was reported for plants propagated on MS medium enriched with 0.5 mg∙dm-3 BAP. The greatest number of the longest roots was developed by stevia on the MS medium enriched with 1 mg∙dm-3 IAA.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Plant growth regulators; Cytokinins; Auxins; Steviosides.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000100403

Flask sealing on in vitro seed germination and morphogenesis of two types of ornamental pepper explants
Provedor de dados: 65
Autores: Batista,Diego Silva; Dias,Leonardo Lucas Carnevalli; Rêgo,Mailson Monteiro do; Saldanha,Cleber Witt; Otoni,Wagner Campos.
ABSTRACT: The influence of flask sealing and explant source on the in vitro morphogenesis of pepper, were evaluated in Capsicum annuum. Seeds were sterilized and inoculated in Murashige and Skoog media supplemented with vitamins, myo-inositol, sucrose, and agar. Gas exchange was evaluated in the germination stage by comparing 3 flask-sealing systems: rigid polypropylene lids (PLs) without vents, PLs with 1 vent, and PLs with 2 vents covered with membranes. In the regeneration stage, cotyledon and hypocotyl segments were transferred to the organogenesis-inducing media, being the different sealing types also tested in a factorial scheme. Photosynthetic pigments, morphological and hystological analyses were conducted for each treatment. Plants maintained in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Gas exchange; Ornamental plant; Capsicum annuum.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300402

In vitro establishment of blackberry (Rubus sp.) cultivar 'Xavante'
Provedor de dados: 65
Autores: Pelizza,Tânia Regina; Silveira,Fabiane Nunes; Ribeiro,Roberta Sabatino; Machado,Bruno Dalazen; Rufatto,Leo; Kretzschmar,Aike Anneliese.
ABSTRACT: The objective of this study was to evaluate the in vitro establishment of 'Xavante' blackberry under different conditions of plant luminosity and concentrations of salts of MS medium. The maintenance of 'Xavante' blackberry plants in the absence of light, with the use of MS medium with salt concentrations of 75% and 100% reduce bacterial contamination and the percentage of explants oxidized and provide the highest percentage of surviving and established explants. The 50% salt dilution in MS medium is not recommended when plants are subjected to the absence of light due to the high percentage of oxidized explants and consequently lower survival and establishment.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Contamination; Luminosity; In vitro maintenance; Micropropagation; Oxidation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901542

Ex vitro acclimatization of Hippeastrum reticulatum (Amaryllidaceae) using different substrates
Provedor de dados: 65
Autores: Heintze,Willian; Milani,Marília; Pradella,Elisandra Maria; Fior,Claudimar Sidnei; Schafer,Gilmar.
ABSTRACT: Hippeastrum spp. are herbaceous species belonging to the family Amaryllidaceae, widely marketed worldwide as indoor plants. Aiming to characterize chemically and physically and to compare five substrates in acclimatization, micropropagated seedlings of Hippeastrum reticulatum var. striatifolium (Herb), with an average of four roots, two leaves and bulbs with a 4.7mm diameter, were transplanted to 50-cell polyethylene trays each containing the following substrates: T1 - vermiculite, T2 - carbonized rice husk (CRH), T3 - commercial (peat + vermiculite + CRH), T4 - coconut fiber, and T5 - composted Pinus bark. After transplantation, the trays were kept for 17 days at a greenhouse with intermittent irrigation by nebulization, and 80 days at a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Seedling production; Floriculture.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001200401

THE EFFICIENCY OF SHOOT AND PLANTLET FORMATION OF Cephaelis ipecacuanha AFTER THREE SUBCULTURES IN VITRO
Provedor de dados: 65
Autores: Lameira,Osmar Alves; Costa,Marly Pedroso da; Pinto,José Eduardo Brasil Pereira.
Multiple adventitious shoot formed from internodal segments of Cephaelis ipecacuanha cultured 25 days on Gamborg basal medium (GAMBORG et al., 1968) supplemented with 6.66mM 6-benzylaminopurine there was a maximum of nine shoots per segment and an average of five shoots per segment formed. The presence of gibberellic acid in the subculture media promoted shoot elongation in all treatments. The shoots attained 3cm in height and rooting of 100% after 35 days of culturing upon Murashige and Skoog's basal medium (MS), added with 4.92mM indole-3-butyric acid, 0.87m gibberellic acid and 0.1% activated charcoal. Further growth was accelerated after the transfer to 1/2 MS without growth regulators. Rooted plantlets transferred to potting soil could be successfully...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plant; Micropropagation; Ipecac.
Ano: 1994
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781994000300013

Kasugamycin on Leifsonia xyli subsp. xyli in the in vitro culture of sugarcane
Provedor de dados: 65
Autores: Garcia,Fabio Ribeiro; Cavalcante,Felipe Lira de Sá; Souza,Robson Antonio de; Houllou,Laureen Michelle.
ABSTRACT:  One of the significant obstacles to the growth of sugarcane production is the infection by phytopathogens, mainly by the bacterium Leifsonia xyli subsp. xyli (Lxx) causal agent of Ratoon stunting disease. Thus, this research aimed to evaluate the effects of kasugamycin on the in vitro growth of sugarcane, as well as its effect on the bacterium Lxx. Explants of strain SP791011 from sugarcane were inoculated in MS culture medium supplemented with the antimicrobial kasugamycin at concentrations of 0.00; 0.87; 1.08; 1.74 and 3.48 mL.L-1, where they remained for 30 days. After this period, the survival rate, shoot number per explant, height of the explants, phytomass, dry phytomass and phytosanitary were evaluated based on the presence of genomic DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Endophytic; Ratoon stunting disease.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000800404

Decontaminant solution on in vitro growth of Byrsonima intermedia seedlings
Provedor de dados: 65
Autores: Silva,Luciano Coutinho; Paiva,Renato; Vargas,Daiane Peixoto; Silva,Diogo Pedrosa Corrêa da; Barbosa,Sandro; Herrera,Raírys Cravo.
Byrsonima intermedia A. Juss. é uma planta medicinal e frutífera do Cerrado, cuja propagação convencional é difícil, devido à presença do endocarpo extremamente lignificado. Embora o hipoclorito de sódio (NaOCl) seja amplamente utilizado para a desinfestação superficial, existem poucos relatos de seus efeitos sobre o crescimento do explante. Neste trabalho, o objetivo foi estudar o efeito de diferentes pHs e períodos de exposição de sementes de B. intermedia em uma solução de NaOCl. As sementes foram submetidas a diferentes tempos de exposição (1, 5 e 10 minutos) numa solução de NaOCl com diferentes pHs (5, 7, 10 e 12) e, após o tratamento com hipoclorito de sódio, os embriões foram inoculados em meio WPM com a concentração de 50% de sais sem sacarose,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Native plant; Tissue culture; PH; NaOCl.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000400674

Callus induction in leaf segments of Croton urucurana Baill
Provedor de dados: 64
Autores: Lima,Ednabel Caracas; Paiva,Renato; Nogueira,Raírys Cravo; Soares,Fernanda Pereira; Emrich,Eduardo Bucsam; Silva,Álvaro Augusto Naves.
Croton urucurana Baill., a species belonging to the family Euphorbiaceae, can be useful in the recovery of degraded riparian areas. In the traditional medicine, it is widely used as cicatrizant and in the treatment of rheumatism. However, its seeds present dormancy and low viability, making the propagation of this species a challenge. With the objective of establishing an alternative route for the propagation, the effect of different concentrations of 2,4-D (2,4- dichlorophenoxyacetic acid) with BAP (6-benzylaminopurine) or TDZ (thidiazuron) and of NAA (1-naphthaleneacetic acid) with BAP and their possible combinations were evaluated for callus induction in leaf segments. Callus fresh mass was evaluated forty-five days after inoculation. The isolated use...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Calogenesis; Tissue culture.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000100002

Effect of different carbon sources on the in vitro multiplication of Annona sp.
Provedor de dados: 64
Autores: Santana,José Raniere Ferreira de; Paiva,Renato; Souza,Ana Valéria de; Oliveira,Lenaldo Muniz de.
The Annonaceae family comprises approximately 2.300 species, some with significant commercial value. Although commercial plantations have suffered due to problems related to seedling production. As micropropagation is a viable technique for seedling production, the present work evaluated the effects of different carbon sources on in vitro bud induction in five Annonaceae species. Nodal segments obtained from plants of the Annona glabra, A. cauliflora, A. coriacea, A. bahiensis and Rollinia silvatica species were inoculated into solid WPM culture medium with 8.87 μM BAP, 0.86 mM of benomyl, and 87.64 mM of the following carbon sources: glucose, sucrose, fructose, galactose, sorbitol and maltose. We evaluated the buds number, the length and weight of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Annonaceae; Micropropagation; Carbohydrates.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000300008

In vitro morphogenesis of Syngonanthus mucugensis Giul: subsp. mucugensis
Provedor de dados: 64
Autores: Lima-Brito,Alone; Resende,Sheila Vitória; Lima,Carolina Oliveira de Cerqueira; Alvim,Bruno Matos; Carneiro,Claudia Elena; Santana,José Raniere Ferreira de.
Syngonanthus mucugensis Giul. subsp. mucugensis is an herbaceous plant with significant economic value in the ornamental dry flower business. The restricted occurrence of the municipality Mucugê-BA, Brazil, exclusively associated with extractive exploitation, has considered this species as endangered. The objective of this work was to evaluate the organogenic potential of three different types of S. mucugensis subsp. mucugensis explants to promote the development of an alternative method to the propagation of the genetic resources of this important plant. The morphogenetic capacities of the leaf, stem and root this species was tested using Murashige and Skoog culture medium at half salt concentration and different concentrations of growth of regulators...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue culture; Organogenesis; Micropropagation; Straw-flowers.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000300010

In vitro propagation and acclimatization of genipapo accessions
Provedor de dados: 64
Autores: Sa,Francielen Paola de; Ledo,Ana da Silva; Amorim,Julie Anne Espindola; Silva,Ana Veruska Cruz da; Pasqual,Moacir.
ABSTRACT Genipapo is a species with an intermediate seed storage behavior, and its germination is slow and asynchronous; therefore the tissue culture is an alternative strategy for the propagation in large-scale. This study aimed to evaluate three genipapo accessions at different micropropagation stages (adventitious shoot induction, in vitro rooting and acclimatization). Genipapo nodal segments from the Sabinópolis (AS), Cruz das Almas (CZA) and Núcleo Bandeirante (NB) accessions were used and inoculated in culture media with 0 and 1.0 mg L-1 6-benzylaminopurine (BAP). Different indole-3-butyric acid (IBA) concentrations were tested for in vitro rooting. Subsequently, the effect of two substrates on the seedling development of three genipapo accessions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genipa americana; In vitro culture; Micropropagation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542016000200155

In vitro propagation of Campomanesia rufa: An endangered fruit species
Provedor de dados: 64
Autores: Sant’Ana,Cecília Ramos de Oliveira; Paiva,Renato; Reis,Michele Valquíria dos; Silva,Diogo Pedrosa Corrêa da; Silva,Luciano Coutinho.
ABSTRACT The Campomanesia rufa is a fruitful species native from Brazil considered as endangered by the IUC (International Union for Conservation of Nature) and low information regarding its propagation is available. In this context, the aim of the present study was to develop in vitro germination, micropropagation and callogenesis protocols for the species. For in vitro germination, seeds were inoculated in MS medium supplemented with GA3 (gibberellic acid) and for shoot induction, the medium was supplemented with three different cytokinins BA (benzyladenine), BAP (6-Benzylaminopurine) or TDZ (Thidiazuron). For shoot growth, culture medium containing BA, BAP, TDZ were maintained under white fluorescent lamps and blue:red light-emitting diodes (LED). GA3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue culture; Micropropagation; Myrtaceae; Casaqueira.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542018000400372

Culture media for the multiplication of wild Manihot species
Provedor de dados: 64
Autores: Sá,Jucieny Ferreira de; Sampaio,Emília dos Santos; Mendes,Maria Inês de Souza; Santos,Karen Cristina Fialho dos; Sousa,Antônio da Silva; Ledo,Carlos Alberto da Silva.
ABSTRACT The cassava propagation system is slow and favors disease transmission through successive generations. Micropropagation is an alternative to overcome the aforementioned limitations, besides allowing the generation of a larger number of pest- and pathogen-free plants. Therefore, the aim of the present study is to investigate the effect of culture media on the multiplication in vitro of five wild Manihot species. The experiment followed a completely randomized design, at factorial arrangement 5 (wild Manihot species) x 6 (culture media), with 11 repetitions. Explants consisted in nodal segments (91 cm long and one lateral bud) of species Manihot flabellifolia, M. tristis, M. caerulescens, M. chlorosticta and M. jacobinensis, which were extracted in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cassava; Wild parental; Nutritional media; Tissue culture; Micropropagation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542018000600598