Consulta: subjectFacets:"Microsatellites"
Registros recuperados: 149
Data/hora: 10/06/2024 02:57:07

Análisis de la variación infraespecífica de Vanilla insignis Ames bajo el enfoque de recursos genéticos.
Provedor de dados: 32
Autores: Hernández Silva, Néstor.
En la actualidad los efectos negativos de las interacciones bióticas y abióticas dentro del cultivo de Vanilla planifolia han devastado plantaciones de este recurso en todo el mundo. La generación de estrategias para su conservación y mejoramiento requiere del conocimiento de la variación que el género Vanilla alberga. Vanilla insignis es una especie silvestre genéticamente relacionada, con características agronómicas sobresalientes dentro del pool genético secundario de V. planifolia, particularmente en la resistencia a factores climáticos y patógenos. Por tal razón, se planteó conocer la variación infraespecífica de V. insignis por medio de la caracterización morfológica y molecular de poblaciones de V. insignis del estado de Quintana Roo, México. A...
Palavras-chave: Conservación; Mejoramiento; Labelo; Microsátelites; Morfotipo; Genotipo; Conservation; Improvement; Labellum; Microsatellites; Morphotype; Genotype; Estrategias para el Desarrollo Agrícola Regional; EDAR; Maestría.
Ano: 2014
URL: http://hdl.handle.net/10521/2230

Development of trinucleotide (GGC)n SSR markers in peanut (Arachis hypogaea L.)
Provedor de dados: 69
Autores: Yuan,Mei; Gong,Limin; Meng,Ronghua; Li,Shuangling; Dang,Phat; Guo,Baozhu; He,Guohao.
Cultivated peanut (Arachis hypogaea L.) is an oilseed crop of economic importance. It is native to South America, and it is grown extensively in the semi-arid tropics of Asia, Africa, and Latin America. Given an extremely narrow genetic base, efforts are being made to develop simple sequence repeat (SSR) markers to provide useful genetic and genomic tools for the peanut research community. A SSR-enriched library to isolate trinucleotide (GGC)n SSRs in peanut was constructed. A total of 143 unique sequences containing (GGC)n repeats were identified. One hundred thirty eight primer pairs were successfully designed at the flanking regions of SSRs. A suitable polymerase was chosen to amplify these GC-rich sequences. Although a low level of polymorphism was...
Tipo: Journal article
Palavras-chave: Cultivated peanut; Microsatellites; Polymorphism; Simple sequence repeat.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600005

Genetic differentiation between Cinta Senese and commercial pig breeds using microsatellite
Provedor de dados: 69
Autores: Scali,Monica; Vignani,Rita; Bigliazzi,Jacopo; Paolucci,Elisa; Bernini,Andrea; Spiga,Ottavia; Niccolai,Neri; Cresti,Mauro.
Background: Cinta Senese (CS) is an autochthonous Tuscan breed, which risked extinction since the ‘60s. Results: Monitoring the genetic variability of the actual population by use of DNA molecular markers is essential to address a correct breeding policy, finalized to obtain the race preservation and its fitness in the future. 17 SSRs autosomal markers and 1 associated to the X chromosome were used to genotype 86 individuals belonging to the CS and 12 belonging to two main white races Landrace (L), Large White (LW) and crosses between LW and L and L and CS widespread in Tuscany and used in the recent past to obtain hybrids with the CS. Conclusions: A dendrogram of similarity measures the relative genetic distance between individuals in the population. Data...
Tipo: Journal article
Palavras-chave: Cinta Senese; Genetic diversity; Genotyping; Microsatellites; Sus scrofa.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200001

Decline of genetic variability in a captive population of Pacific white shrimp Penaeus (Litopenaeus) vannamei using microsatellite and pedigree information
Provedor de dados: 69
Autores: Vela Avitúa,Sergio; Montaldo,Hugo H; Márquez Valdelamar,Laura; Campos Montes,Gabriel R; Castillo Juárez,Héctor.
Background: The objective of this study was to estimate the decline of genetic variability and the changes in effective population size in three shrimp populations. One was a wild population collected at several points in the Mexican Pacific Ocean. The other two populations were different generations (7 and 9) from a captive population selected for growth and survival. Microsatellite markers and pedigree were both used to assess genetic variability and effective population size. Results: Using 26 loci, both captive populations showed a decline in the expected heterozygosity (20%) and allelic diversity indices (48 to 91%) compared to the wild population (P &lt; 0.05). The studied captive populations did not differ significantly from each other regarding...
Tipo: Journal article
Palavras-chave: Effective population size; Genetic diversity; Heterozygosity; Microsatellites; Selection; Shrimp.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400009

SSR genetic diversity assessment of popular pigeonpea varieties in Malawi reveals unique fingerprints
Provedor de dados: 69
Autores: Njung'e,Vincent; Deshpande,Santosh; Siambi,Moses; Jones,Richard; Silim,Said; De Villiers,Santie.
Background: Pigeonpea (Cajanus cajan (L.) Millsp.) is a drought tolerant legume of the Fabaceae family and the only cultivated species in the genus Cajanus. It is mainly cultivated in the semi-arid tropics of Asia and Oceania, Africa and America. In Malawi, it is grown as a source of food and income and for soil improvement in intercropping systems. However, varietal contamination due to natural outcrossing causes significant quality reduction and yield losses. In this study, 48 polymorphic SSR markers were used to assess the diversity among all pigeonpea varieties cultivated in Malawi to determine if a genetic fingerprint could be identified to distinguish the popular varieties. Results: A total of 212 alleles were observed with an average of 5.58 alleles...
Tipo: Journal article
Palavras-chave: Genetic variation; Genotyping; Microsatellites; Seed purity.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300008

Genetic diversity analysis in the section Caulorrhizae (genus Arachis) using microsatellite markers
Provedor de dados: 74
Autores: Palmieri,Darío A.; Bechara,Marcelo D.; Curi,Rogério A.; Monteiro,Jomar P.; Valente,Sérgio E.S.; Gimenes,Marcos A.; Lopes,Catalina R.
Diversity in 26 microsatellite loci from section Caulorrhizae germplasm was evaluated by using 33 accessions of A. pintoi Krapov. &amp;amp; W.C. Gregory and ten accessions of Arachis repens Handro. Twenty loci proved to be polymorphic and a total of 196 alleles were detected with an average of 9.8 alleles per locus. The variability found in those loci was greater than the variability found using morphological characters, seed storage proteins and RAPD markers previously used in this germplasm. The high potential of these markers to detect species-specific alleles and discriminate among accessions was demonstrated. The set of microsatellite primer pairs developed by our group for A. pintoi are useful molecular tools for evaluating Section Caulorrhizae...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arachis; Genetic diversity; Germplasm; Microsatellites; Molecular markers.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100019

Genetic variability in Amerindian populations of Northern Argentina
Provedor de dados: 74
Autores: Dejean,Cristina B.; Crouau-Roy,Brigitte; Goicoechea,Alicia S.; Avena,Sergio A.; Carnese,Francisco R.
The allelic variability of four dinucleotide microsatellites located in the HLA region (MOGc, D6S265, MIB, and TNFa) was analyzed in 67 individuals representing three Amerindian populations of the Argentine Gran Chaco: Toba, Wichi and Chorote. Genomic DNA was prepared from peripheral blood and DNA was extracted using the standard phenol-chloroform procedure. Alleles were identified by PCR, using an end-labelled reverse oligonucleotide primer (fluorescent 6 - Fam labeling). Despite the low number of samples studied, a high level of gene diversity was observed in each population and for each locus. Moreover, the mean number of alleles was 7.7, 5.3, 10.0, and 7.0 at loci MOGc, D6S265, MIB and TNFa, respectively. Differentiation tests between pairs of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aboriginal Argentinians; Microsatellites; Intra- and inter-populational variation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400004

Evidence of high inbreeding in a population of the endangered giant anteater, Myrmecophaga tridactyla (Myrmecophagidae), from Emas National Park, Brazil
Provedor de dados: 74
Autores: Collevatti,Rosane G.; Leite,Kelly C.E.; Miranda,Guilherme H.B. de; Rodrigues,Flavio H.G.
We report the genetic structure, relatedness and mating structure of a population of the endangered giant anteater Myrmecophaga tridactyla Linnaeus, 1758 in the Emas National Park, Brazil, based on variability at five microsatellite loci. Additionally, we addressed the hypothesis that the M. tridactyla population studied has low levels of polymorphism and high levels of inbreeding and relatedness and that animals with overlapping home range are highly related. All five microsatellite loci displayed low levels of polymorphism and of expected and observed heterozygosity. The low level of polymorphism and high inbreeding showed by the population studied may be the outcome of high mortality and reduction in population size due to recurrent fire events in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerrado; Conservation; Microsatellites; Phylopatry; Relatedness.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100020

Multiple introductions and gene flow in subtropical South American populations of the fireweed, Senecio madagascariensis(Asteraceae)
Provedor de dados: 74
Autores: Mäder,Geraldo; Castro,Luana; Bonatto,Sandro Luis; Freitas,Loreta Brandão de.
Abstract Non-indigenous plants exhibit different attributes that make them aggressive competitors with indigenous plants and serious threats to biodiversity.Senecio madagascariensis (fireweed, Asteraceae), a native from southern Africa, is a strong competitor in agricultural activities and has toxic alkaloids that may result in high cattle mortality. In Brazil, this weed was collected for the first time in 1995 and has since spread quickly throughout the Pampas region. To better understand the invasion of the fireweed in South America, we used a genetic characterization with internal transcribed spacer (ITS) and microsatellite markers. Based on the ITS data, the southern Brazil populations of S. madagascariensis shared genetic homology with samples taken...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; ITS; Pampas; Weed; Spread.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100135

Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci
Provedor de dados: 74
Autores: Mantellatto,Aline Meira Bonfim; Caparroz,Renato; Christofoletti,Maurício Durante; Piovezan,Ubiratan; Duarte,José Maurício Barbanti.
Abstract The pampas deer (Ozotoceros bezoarticus) is close to being classified as ‘globally threatened’, with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Fecal DNA; Microsatellites; Non-invasive methods; Pampas deer.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500774

Microsatellite-assisted backcross selection in maize
Provedor de dados: 74
Autores: Benchimol,Luciana Lasry; Souza Jr.,Cláudio Lopes de; Souza,Anete Pereira de.
A microsatellite marker (SSR) was chosen to simulate a target allele and three criteria (02, 04 and 06 markers per chromosome) were tested to evaluate the most efficient parameters for performing marker-assisted backcross (MAB) selection. We used 53 polymorphic SSRs to genotype 186 BC1 maize (Zea mays L.) plants produced by crossing the inbred maize lines L-08-05 (donor parent) and L-14-4B (recurrent parent). The second backcross (BC2) generation was produced with 180 plants and screened with markers which were not recovered from the first backcross (BC1) generation. A total of 480 plants were evaluated in the third backcross (BC3) generation from which 48 plants were selected for parental genotype recovery. Recurrent genotype recovery averages in three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maize; Marker-assisted backcrossing (MAB); Marker-assisted selection; Microsatellites.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500022

Characterization of the complete mitochondrial genome and a set of polymorphic microsatellite markers through next-generation sequencing for the brown brocket deer Mazama gouazoubira
Provedor de dados: 74
Autores: Caparroz,Renato; Mantellatto,Aline M.B.; Bertioli,David J.; Figueiredo,Marina G.; Duarte,José Maurício B.
The complete mitochondrial genome of the brown brocket deer Mazama gouazoubira and a set of polymorphic microsatellite markers were identified by 454-pyrosequencing. De novo genome assembly recovered 98% of the mitochondrial genome with a mean coverage of 9-fold. The mitogenome consisted of 16,356 base pairs that included 13 protein-coding genes, two ribosomal subunit genes, 22 transfer RNAs and the control region, as found in other deer. The genetic divergence between the mitogenome described here and a previously published report was ∼0.5%, with the control region and ND5 gene showing the highest intraspecific variation. Seven polymorphic loci were characterized using 15 unrelated individuals; there was moderate genetic variation across most loci (mean...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Microsatellites; Mitogenome; Population genetics; 454-pyrosequencing.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300338

Origin, evolution and genome distribution of microsatellites
Provedor de dados: 74
Autores: Oliveira,Eder Jorge; Pádua,Juliano Gomes; Zucchi,Maria Imaculada; Vencovsky,Roland; Vieira,Maria Lúcia Carneiro.
Microsatellites, or simple sequence repeats (SSRs), have been the most widely applied class of molecular markers used in genetic studies, with applications in many fields of genetics including genetic conservation, population genetics, molecular breeding, and paternity testing. This range of applications is due to the fact that microsatellite markers are co-dominant and multi-allelic, are highly reproducible, have high-resolution and are based on the polymerase chain reaction (PCR). When first introduced, the development of microsatellite markers was expensive but now new and efficient methods of repetitive sequence isolation have been reported, which have led to reduced costs and microsatellite-technology has been increasingly applied to several species,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Molecular genetics; Genetic structure of populations.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200018

Genetic diversity and population structure of naturally rare Calibrachoa species with small distribution in southern Brazil
Provedor de dados: 74
Autores: John,Ana Laura de Wallau; Mäder,Geraldo; Fregonezi,Jeferson N.; Freitas,Loreta B.
Abstract Calibrachoa is a South-American genus comprising 27 species, several considered endemic or rare; few were subjects in genetic studies. We attempted to generate new data about the phylogenetically related and rare species C. eglandulata, C. sendtneriana, C. serrulata, and C. spathulata concerning their genetic diversity and population structure, which, coupled with their known restricted distribution, could help access their conservation status and contribute to the study of the Brazilian biodiversity. We sequenced 88 individuals for plastid intergenic spacers and genotyped 186 individuals for five microsatellite loci. Compared among each other, C. sendtneriana and C. serrulata presented the highest values of genetic diversity [π% (sd) = 0.23...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plastid DNA; Microsatellites; Threatened species; Grasslands; Herbaceous species.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100108

Molecular genetic diversity in populations of the stingless bee Plebeia remota: A case study
Provedor de dados: 74
Autores: Francisco,Flávio de Oliveira; Santiago,Leandro Rodrigues; Arias,Maria Cristina.
Genetic diversity is a major component of the biological diversity of an ecosystem. The survival of a population may be seriously threatened if its genetic diversity values are low. In this work, we measured the genetic diversity of the stingless bee Plebeia remota based on molecular data obtained by analyzing 15 microsatellite loci and sequencing two mitochondrial genes. Population structure and genetic diversity differed depending on the molecular marker analyzed: microsatellites showed low population structure and moderate to high genetic diversity, while mitochondrial DNA (mtDNA) showed high population structure and low diversity in three populations. Queen philopatry and male dispersal behavior are discussed as the main reasons for these findings.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA; Meliponini; Microsatellites; Philopatry; Population genetics.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100017

A polymorphic microsatellite from the Squalius alburnoides complex (Osteichthyes, Cyprinidae) cloned by serendipity can be useful in genetic analysis of polyploids
Provedor de dados: 74
Autores: Boto,Luis; Cunha,Carina; Doadrio,Ignacio.
A new microsatellite locus (SAS1) for Squalius alburnoides was obtained through cloning by serendipity. The possible usefulness of this new species-specific microsatellite in genetic studies of this hybrid-species complex, was explored. The polymorphism exhibited by SAS1 microsatellite is an important addition to the set of microsatellites previously used in genetic studies in S. alburnoides complex, that mostly relied in markers described for other species. Moreover, the SAS1 microsatellite could be used to identify the parental genomes of the complex, complementing other methods recently described for the same purpose.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Hybridogenesis; Squalius alburnoides.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300025

Genetic divergence among populations and accessions of the spineless peach palm from Pampa Hermosa landrace used in the heart-of-palm agribusiness in Brazil
Provedor de dados: 74
Autores: Alves-Pereira,Alessandro; Clement,Charles R.; Picanço-Rodrigues,Doriane.
Although originally domesticated for its fruit, exploitation of the peach palm (Bactris gasipaes Kunth) in the production of gourmet heart-of-palm has also become an important activity, hence the need for improved material for large-scale production, on employing the Pampa Hermosa landrace as the seed source. In this study 11 microsatellite markers were used to evaluate genetic divergence among 96 elite plants representing four populations of spineless peach palm from the above cited source. Genetic variability was high (H T = 0.82). The low levels of divergence [F ST (0.023), G ST' (0.005)] and the high number of migrants (Nm -3.8 to 52.2) indicated significant interpopulation gene flow. Some of the plants presented high levels of genetic divergence, but...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bactris gasipaes; Heart-of-palm; Microsatellites; Genetic divergence; Genetic improvement.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300015

Characterization of Brazilian soybean cultivars using microsatellite markers
Provedor de dados: 74
Autores: Priolli,Regina Helena Geribello; Mendes-Junior,Celso Teixeira; Arantes,Neylson Eustáquio; Contel,Eucleia Primo Betioli.
Microsatellite markers or SSR (Simple Sequence Repeats) have proved to be an excellent tool for cultivar identification, pedigree analysis and the evaluation of genetic distance among organisms. Soybean cultivars have been characterized mainly by morphological and biochemical traits. However, these traits have not been sufficient to characterize the large number of cultivars eligible to receive protection under the Brazilian Cultivar Protection Act. In order to define new soybean cultivar markers, the alleles of twelve SSR loci of 186 Brazilian soybean cultivars were studied by estimating the variation in their size range and their respective frequencies. On average, 5.3 alleles per locus were detected, with a mean genetic diversity of 0.64 ± 0.12. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max (L.) Merrill; Simple sequence repeat; Microsatellites; Molecular markers; Soybean elite cultivars.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200012

Genetic characterization of Bhutanese native chickens based on an analysis of Red Junglefowl (Gallus gallus gallus and Gallus gallus spadecieus), domestic Southeast Asian and commercial chicken lines (Gallus gallus domesticus)
Provedor de dados: 74
Autores: Dorji,Nedup; Duangjinda,Monchai; Phasuk,Yupin.
The genetic diversity of Bhutanese chickens needs to be understood in order to develop a suitable conservation strategy for these birds in Bhutan. In this, work, we used microsatellite markers to examine the genetic diversity of Bhutanese chickens. Four Bhutanese chicken varieties (Black plumage, Frizzle, Naked neck and Red Junglefowllike, corresponding to Yuebjha Narp, Phulom, Khuilay and Seim, respectively), two subspecies of Red Junglefowl (Gallus gallus gallus and Gallus gallus spadecieus), two varieties of Thai native chickens (Pradhu Hang Dam and Chee; Gallus gallus domesticus) representing the Southeast Asian domestic chicken, and two commercial lines (Broiler and Single Comb White Leghorn) were genotyped with 18 microsatellites that included 16...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation genetics; Genetic comparisons; Genetic variability; Microsatellites.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400010

Prediction of social structure and genetic relatedness in colonies of the facultative polygynous stingless bee Melipona bicolor (Hymenoptera, Apidae)
Provedor de dados: 74
Autores: Reis,Evelyze Pinheiro dos; Campos,Lucio Antonio de Oliveira; Tavares,Mara Garcia.
Stingless bee colonies typically consist of one single-mated mother queen and her worker offspring. The stingless bee Melipona bicolor (Hymenoptera: Apidae) shows facultative polygyny, which makes this species particularly suitable for testing theoretical expectations concerning social behavior. In this study, we investigated the social structure and genetic relatedness among workers from eight natural and six manipulated colonies of M. bicolor over a period of one year. The populations of M. bicolor contained monogynous and polygynous colonies. The estimated genetic relatedness among workers from monogynous and polygynous colonies was 0.75 ± 0.12 and 0.53 ± 0.16 (mean ± SEM), respectively. Although the parental genotypes had significant effects on genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melipona; Microsatellites; Polygyny; Queen number; Social structure.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200028

Population structure and genetic variability of mainland and insular populations of the Neotropical water rat, Nectomys squamipes (Rodentia, Sigmodontinae)
Provedor de dados: 74
Autores: Almeida,Francisca C.; Maroja,Luana S.; Moreira,Miguel A.M.; Seuánez,Héctor N.; Cerqueira,Rui.
Seven microsatellite loci were used to investigate the genetic variability and structure of six mainland and two island populations of the Neotropical water rat Nectomys squamipes, a South American semi-aquatic rodent species with a wide distribution. High levels of variability were found within mainland populations while island populations were less variable but the more differentiated in respect to allele number and frequency. The time of biological divergence between mainland and island populations coincided with geological data. A significant geographic structure was found in mainland populations (theta = 0.099; rho = 0.086) although the degree of differentiation was relatively low in respect to the distance between surveyed localities (24 to 740 km)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Population genetics; Islands; Population expansion; Population size; Divergence time.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500008

Genetic variability of Herpailurus yagouaroundi, Puma concolor and Panthera onca (Mammalia, Felidae) studied using Felis catus microsatellites
Provedor de dados: 74
Autores: Moreno,Vanessa Roma; Grisolia,Alexéia Barufatti; Campagnari,Francine; Milazzotto,Marcela; Adania,Cristina Harumi; Garcia,José Fernando; Souza,Edislane Barreiros de.
We used four microsatellite loci (Fca08, Fca45, Fca77 and Fca96) from the domestic cat, Felis catus, to investigate genetic variability in specimens of Herpailurus yagouaroundi (jaguarundi, otter cat, eyra), Puma concolor (cougar, mountain lion, puma) and Panthera onca (jaguar) held in various Brazilian zoos. Samples of DNA from the cats were PCR amplified and then sequenced before being analyzed using the CERVUS program. Our results show a mean polymorphic information content (PIC) of 0.83 for H. yagouaroundi, 0.66 for P. concolor and 0.69 for P. onca and a mean of 10.3 alleles for the Fca08 locus, 5.3 for Fca 45, 9 for Fca 77 and 14 for Fca 96. These results indicate a relatively high level of genetic diversity for the specimens studied.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Mammals; Felines; Microsatellites.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200017

Genomic resources for the conservation and management of the harpy eagle (Harpia harpyja, Falconiformes, Accipitridae)
Provedor de dados: 74
Autores: Banhos,Aureo; Hrbek,Tomas; Gravena,Waleska; Sanaiotti,Tânia; Farias,Izeni P.
We report the characterization and optimization of 45 heterologous microsatellite loci, and the development of a new set of molecular sex markers for the conservation and management of the Neotropical harpy eagle (Harpia harpyja L. 1758). Of the 45 microsatellites tested, 24 were polymorphic, six monomorphic, 10 uncharacterizable due to multiple bands and five did not amplify. The observed gene diversity of the analyzed sample of H. harpyja was low and similar to that of other threatened Falconiformes. While a high proportion of the microsatellite markers were highly variable, individuals of H. harpyja could be differentiated by a joint analysis of just three (p = 2.79 x 10-4) or four markers (p = 2.89 x 10-5). Paternity could be rejected with 95.23% and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation genetics; Harpia harpyja; Microsatellites; Raptors; Sex markers.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100025

Effective population size and the genetic consequences of commercial whaling on the humpback whales (Megaptera novaeangliae) from Southwestern Atlantic Ocean
Provedor de dados: 74
Autores: Cypriano-Souza,Ana Lúcia; da Silva,Tiago Ferraz; Engel,Márcia H.; Bonatto,Sandro L.
Abstract Genotypes of 10 microsatellite loci of 420 humpback whales from the Southwestern Atlantic Ocean population were used to estimate for the first time its contemporary effective (Ne) and census (Nc) population sizes and to test the genetic effect of commercial whaling. The results are in agreement with our previous studies that found high genetic diversity for this breeding population. Using an approximate Bayesian computation approach, the scenario of constant Ne was significantly supported over scenarios with moderate to strong size changes during the commercial whaling period. The previous generation Nc (Ne multiplied by 3.6), which should corresponds to the years between around 1980 and 1990, was estimated between ~2,600 and 6,800 whales (point...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Commercial whaling; Bottleneck; Humpback whale; Microsatellites; Demography.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200253

Repetitive DNAs and shrink genomes: A chromosomal analysis in nine Columbidae species (Aves, Columbiformes)
Provedor de dados: 74
Autores: Kretschmer,Rafael; de Oliveira,Thays Duarte; de Oliveira Furo,Ivanete; Oliveira Silva,Fabio Augusto; Gunski,Ricardo José; del Valle Garnero,Analía; de Bello Cioffi,Marcelo; de Oliveira,Edivaldo Herculano Corrêa; de Freitas,Thales Renato Ochotorena.
Abstract An extensive karyotype variation is found among species belonging to the Columbidae family of birds (Columbiformes), both in diploid number and chromosomal morphology. Although clusters of repetitive DNA sequences play an important role in chromosomal instability, and therefore in chromosomal rearrangements, little is known about their distribution and amount in avian genomes. The aim of this study was to analyze the distribution of 11 distinct microsatellite sequences, as well as clusters of 18S rDNA, in nine different Columbidae species, correlating their distribution with the occurrence of chromosomal rearrangements. We found 2n values ranging from 76 to 86 and nine out of 11 microsatellite sequences showed distinct hybridization signals among...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Birds; FISH; Microsatellites; Sex chromosomes; Chromosomal rearrangements.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100098

Implementation of a parentage control system in Portuguese beef-cattle with a panel of microsatellite markers
Provedor de dados: 74
Autores: Carolino,Inês; Sousa,Conceição O.; Ferreira,Sónia; Carolino,Nuno; Silva,Fátima S.; Gama,Luís T.
A study was conducted to assess the feasibility of applying a panel of 10 microsatellite markers in parentage control of beef cattle in Portugal. In the first stage, DNA samples were collected from 475 randomly selected animals of the Charolais, Limousin and Preta breeds. Across breeds and genetic markers, means for average number of alleles, effective number of alleles, expected heterozygosity and polymorphic information content, were 8.20, 4.43, 0.733 and 0.70, respectively. Enlightenment from the various markers differed among breeds, but the set of 10 markers resulted in a combined probability above 0.9995 in the ability to exclude a random putative parent. The marker-set thus developed was later used for parentage control in a group of 140 calves from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Genetic markers; Microsatellites; Parentage control.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200017

Evidence of lack of population substructuring in the Brazilian freshwater fish Prochilodus costatus
Provedor de dados: 74
Autores: Carvalho-Costa,Luis F.; Hatanaka,Terumi; Galetti Jr.,Pedro M.
Curimbatá-pióa (Prochilodus costatus Valenciennes, 1850) is an endemic species from the São Francisco River Basin with migratory habits, important ecological role in the ecosystem and relevant fishing importance. The present study aimed to assess the genetic variation in P. costatus in order to investigate its population genetic structure. Genetic variation was studied at three sites downstream the Três Marias dam (Upper-middle São Francisco River, State of Minas Gerais) through six specific microsatellite loci. Fish from the three sites had quite similar genetic diversity levels and no genetic differentiation was detected, suggesting that P. costatus might represent a single reproductive unit in the studied area. Alternatively, the present study was not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prochilodontidae; Curimbatá; Microsatellites; Genetic variation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200036

Genetic structure of Brazilian wild rice (Oryza glumaepatula Steud., Poaceae) populations analyzed using microsatellite markers
Provedor de dados: 74
Autores: Karasawa,Marines M.G.; Vencovsky,Roland; Silva,Cynthia M.; Zucchi,Maria Imaculada; Oliveira,Giancarlo C.X.; Veasey,Elizabeth A.
Knowledge of the genetic structure and diversity of natural populations is important in developing strategies for in situ and ex situ conservation. We used eight microsatellite loci to estimate genetic structure and investigate within and between population genetic variation in eleven Brazilian wild rice (Oryza glumaepatula) populations. The study showed the following genetic diversity parameters: average number of 3.1 alleles per locus; 77.3% polymorphic loci; 0.091 observed heterozygosity and 0.393 gene diversity. F-statistics detected by microsatellite loci were: F ST = 0.491 (and R ST = 0.608), F IS = 0.780 and F IT = 0.888. No population was in Hardy-Weinberg equilibrium. The estimated apparent outcrossing rate (0.143) indicated a predominance of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Gene flow; Genetic diversity; Microsatellites; Oryza glumaepatula.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300017

Challenges and prospects of population genetic studies in terns (Charadriiformes, Aves)
Provedor de dados: 74
Autores: Faria,Patrícia J.; Baus,Erika; Morgante,João S.; Bruford,Michael W.
Little information is available about the population structure of communally nesting terns (Sternidae) and skimmers (Rynchopidae) throughout the world. In order to fill this gap, a survey of molecular markers was carried out for six species of terns (Anous stolidus, Sterna hirundinacea, S. fuscata, S. superciliaris, Thalasseus maximus and Phaetusa simplex) and one species of skimmer (Rynchops niger). First, we describe the results of the construction of genomic DNA libraries and document problems encountered during this procedure. Secondly, we tested the cross-amplification of 18 microsatellite loci previously described for related species (the number of polymorphic loci ranged from three to seven). Thirdly, we tested the usefulness of mtDNA (control...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; Microsatellites; Mitochondrial DNA; Terns.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400029

Microsatellite characterization of Cimarron Uruguayo dogs
Provedor de dados: 74
Autores: Gagliardi,Rosa; Silvia,Llambí; García,Cristina; Arruga,María Victoria.
Various genetic markers, including microsatellites, have been used to analyze the genetic polymorphism and heterozygosity in canine breeds. In this work, we used nine microsatellite markers to investigate the genetic variability in Cimarron Uruguayo dogs, the only officially recognized native canine breed in Uruguay. DNA from 30 Cimarron Uruguayo dogs from northeastern and southern Uruguay was analyzed. The allelic frequencies for each micro-satellite, the genetic variability and the consanguinity were calculated, as were the polymorphic information content (PIC) and the probability of exclusion (PE). All of the microsatellites studied were polymorphic. FH 2361, FH 2305 and PEZ 03 were the most informative, with PIC values &gt; 0.7, in agreement with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cimarron Uruguayo dog; Genetic characterization; Microsatellites.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100028

Using genetic diversity information to establish core collections of Stylosanthes capitata and Stylosanthes macrocephala
Provedor de dados: 74
Autores: Santos-Garcia,Melissa Oliveira; Toledo-Silva,Guilherme de; Sassaki,Rodrigo Possidonio; Ferreira,Thais Helena; Resende,Rosângela Maria Simeão; Chiari,Lucimara; Karia,Cláudio Takao; Carvalho,Marcelo Ayres; Faleiro,Fábio Gelape; Zucchi,Maria Imaculada; Souza,Anete Pereira de.
Stylosanthes species are important forage legumes in tropical and subtropical areas. S. macrocephala and S. capitata germplasm collections that consist of 134 and 192 accessions, respectively, are maintained at the Brazilian Agricultural Research Corporation Cerrados (Embrapa-Cerrados). Polymorphic microsatellite markers were used to assess genetic diversity and population structure with the aim to assemble a core collection. The mean values of H O and H E for S. macrocephala were 0.08 and 0.36, respectively, whereas the means for S. capitata were 0.48 and 0.50, respectively. Roger's genetic distance varied from 0 to 0.83 for S. macrocephala and from 0 to 0.85 for S. capitata. Analysis with STRUCTURE software distinguished five groups among the S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stylosanthes; Tropical forage; Microsatellites; Genetic diversity; Core collection.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500020

Paternity testing and behavioral ecology: a case study of jaguars (Panthera onca) in Emas National Park, Central Brazil
Provedor de dados: 74
Autores: Soares,Thannya Nascimento; Telles,Mariana P.C.; Resende,Lucileide V.; Silveira,Leandro; Jácomo,Anah Tereza A.; Morato,Ronaldo G.; Diniz-Filho,José Alexandre F.; Eizirik,Eduardo; Brondani,Rosana P.V.; Brondani,Claudio.
We used microsatellite loci to test the paternity of two male jaguars involved in an infanticide event recorded during a long-term monitoring program of this species. Seven microsatellite primers originally developed for domestic cats and previously selected for Panthera onca were used. In order to deal with uncertainty in the mother's genotypes for some of the loci, 10000 values of W were derived by simulation procedures. The male that killed the two cubs was assigned as the true sire. Although the reasons for this behavior remain obscure, it shows, in principle, a low recognition of paternity and kinship in the species. Since the two cubs were not very young, one possibility is that the adult male did not recognize the cubs and killed them for simple...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ecological stress; Infanticide; Jaguar; Microsatellites; Paternity.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400025

DNA testing for parentage verification in a conservation nucleus of Pantaneiro horse
Provedor de dados: 74
Autores: Sereno,Fabiana Tavares Pires de Souza; Sereno,José Robson Bezerra; Vega-Pla,Jose Luis; Delgado,Juan Vicente.
We investigated the genealogy of the in situ conservation nucleus of the Pantaneiro horse using DNA microsatellites by evaluating 101 horses, the group consisting of 71 adult horses (3 stallions, 40 male and 31 mares) and 27 foals (14 colts and 13 fillies). Genomic DNA was extracted from hair roots and genotyped using 12 microsatellite markers (AHT4, AHT5, ASB2, ASB17, ASB23, HMS3 HMS6, HMS7, HTG4, HTG10, LEX33 and VHL20). The number of alleles per locus varied from 6 to 13, with a mean of 7.8 and the expected heterozygosity ranged from 0.544 to 0.734 (mean 0.644). The VLH20, ASB2, HTG10, ASB23 markers had a high (&gt; 0.8) polymorphism information content and the total exclusion probability of the 12 microsatellite loci was 0.99. The genealogical study of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Pantaneiro horse; Animal conservation; Microsatellites; Markers; Parentage verification.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100013

Genetic diversity in populations of African mahogany (Khaya grandioliola C. DC.) introduced in Brazil
Provedor de dados: 74
Autores: Soares,Sabrina Delgado; Bandeira,Ludmila Ferreira; Ribeiro,Stela Barros; Telles,Mariana Pires de Campos; Silva,João Augusto da; Borges,Canrobert Tormin; Coelho,Alexandre Siqueira Guedes; Novaes,Evandro.
Abstract Given its high-valued wood, the African mahogany (Khaya grandifoliola) has been envisaged as a renewable source of tropical hardwoods in Brazil. However, there are concerns about the hypothesized low diversity among the few K. grandifoliola germplasm sources introduced in the country. Using eight microsatellite markers, we evaluated the genetic diversity and divergence among 53 superior trees selected from three provenances of K. grandifoliola located in the state of Para. These populations are among the oldest plantations (&gt;15 years) in Brazil and, therefore, the country's main seed sources. The average number of alleles per locus was 5.9, expected heterozygosity was moderate (^=0.56) and lower than the high observed heterozygosity (HO=0.74)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; SSR; Genetic structure; Genetic divergence; Hardwood.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400301

Microsatellite analysis of a sample of Uruguayan Creole bulls (Bos taurus)
Provedor de dados: 74
Autores: Armstrong,Eileen; Postiglioni,Alicia; Martínez,Amparo; Rincón,Gonzalo; Vega-Pla,José Luis.
The Uruguayan Creole cattle genetic reserve consists of a herd of about 600 animals (bulls, cows and calves) located in an indigenous habitat of 650 hectares. In a previous study, a random sample from this herd showed high heterozygosity and a Hardy-Weinberg equilibrium for markers of major genes related to milk production. To study its genetic diversity we genotyped a sample of bulls (N = 19 out of 23 for the whole herd) using the PCR reaction with a set of 17 microsatellite markers. Between two and seven different alleles were identified per microsatellite in a total of 73 alleles. The expected mean heterozygosity (He) per locus was between 0.465 and 0.801, except for microsatellite HEL13 which gave a He value of 0.288. The expected mean heterozygosity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Uruguayan Creole cattle; Genetic diversity; Microsatellites.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200012

Genetic differentiation in geographically close populations of the water rat Nectomys squamipes (Rodentia, Sigmodontinae) from the Brazilian Atlantic Forest
Provedor de dados: 74
Autores: Maroja,L.S.; Almeida,F.C.; Seuánez,H.N.
We examined the genetic structure and the effects of a bottleneck in populations of the water rat Nectomys squamipes, a primary host of Schistosoma mansoni. Eight microsatellite loci were studied in 7 populations from the Sumidouro region of the Brazilian state of Rio de Janeiro. Our data, covering a four-year period during which a bottleneck occurred, revealed substantial variation (6-31 alleles per locus) and high levels of both observed (0.718-0.789) and expected (0.748-0.832) heterozygosity. Most populations were in Hardy-Weinberg equilibrium without linkage disequilibrium between loci. Overall average genetic differentiation between populations (estimated with the F ST (q) and R ST (r) analogues was 0.037 for q and 0.060 for r. There was significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Migration; Population genetics; Schistosoma mansoni host; Sigmodontinae.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400002

Genetic characterization of the Spanish Trotter horse breed using microsatellite markers
Provedor de dados: 74
Autores: Javier Azor,Pedro; Valera,Mercedes; Gómez,María Dolores; Goyache,Félix; Molina,Antonio.
To assist in selection schemes we carried out the first genetic characterization of the Spanish Trotter horse (Trotador Español). We used 16 microsatellite markers to genotype 40 unrelated Spanish trotters, 25 native Balearic horses (11 Menorquina and 14 Mallorquina horses) and 32 Andalusian horses. The observed heterozygosity for the Spanish Trotters was 0.647 ± 0.037 and the expected heterozygosity was 0.696 ± 0.026 while the average number of alleles per locus was 6.0 ± 0.341, these values being similar to the data published for other horse breeds. We also tried to establish the importance of the Mallorquina and Menorquina breeds in the present Spanish Trotter population. Only 9% of the total genetic variability could be attributed to differences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horse; Spanish Trotter breed; Microsatellites; Genetic diferenciation; Migration rates.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100009

Evaluation of polymorphism in ten microsatellite loci in Uruguayan sheep flocks
Provedor de dados: 74
Autores: Tomasco,Ivanna; Wlasiuk,G.; Lessa,E.P.
The allele frequencies of 10 microsatellite loci previously described for sheep as BM1314, BM6526, OarFCB128, OarHH64, OarCP20, OarHH47, OarFCB48, OarHH35, OarHH72 and BM2508 were estimated for the Uruguayan flocks. A representative sample of 101 individuals composed by the two predominant breeds (76% Corriedale and 24% Australian Merino) was used. The sample did not show a significant tendency towards substructuring, in spite of presenting some significantly different allele frequencies between races. The Corriedale sample presents three loci in which the presence of null alleles is possible. The markers were highly variable, showing between 7 and 15 alleles each. The Polymorphism Information Content Index ranged from 0.63 to 0.87 and the Exclusion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sheep; Polymorphism; Microsatellites.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100008

Genetic variability in five populations of Partamona helleri (Hymenoptera, Apidae) from Minas Gerais State, Brazil
Provedor de dados: 74
Autores: Borges,Andreia Arantes; Campos,Lucio Antônio de Oliveira; Salomão,Tânia Maria Fernandes; Tavares,Mara Garcia.
Partamona is a Neotropical genus of stingless bees that comprises 33 species distributed from Mexico to southern Brazil. These bees are well-adapted to anthropic environments and build their nests in several substrates. In this study, 66 colonies of Partamona helleri from five localities in the Brazilian state of Minas Gerais (São Miguel do Anta, Teixeiras, Porto Firme, Viçosa and Rio Vermelho) were analyzed using nine microsatellite loci in order to assess their genetic variability. Low levels of observed (Ho = 0.099-0.137) and expected (He = 0.128-0.145) heterozygosity were encountered and revealed discrete genetic differentiation among the populations (F ST =0.025). AMOVA further showed that most of the total genetic variation (94.24%) in P. helleri was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Partamona helleri; Population genetics; Stingless bees.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400028

Genetic characterization of cassava ( Manihot esculenta ) landraces   in Brazil assessed with simple sequence repeats
Provedor de dados: 74
Autores: Siqueira,Marcos V. B. M.; Queiroz-Silva,Jurema R.; Bressan,Eduardo A.; Borges,Aline; Pereira,Kayo J. C.; Pinto,José G.; Veasey,Elizabeth Ann.
Based on nine microsatellite loci, the aim of this study was to appraise the genetic diversity of 42 cassava ( Manihot esculenta ) landraces from selected regions in Brazil, and examine how this variety is distributed according to origin in several municipalities in the states of Minas Gerais, São Paulo, Mato Grosso do Sul, Amazonas and Mato Grosso. High diversity values were found among the five above-mentioned regions, with 3.3 alleles per locus on an average, a high percentage of polymorphic loci varying from 88.8% to 100%, an average of 0.265 for observed heterozygosity and 0.570 for gene diversity. Most genetic diversity was concentrated within the regions themselves ( H   S   = 0.52). Cluster analysis and principal component based scatter plotting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Microsatellites; SSR markers; Traditional farming.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100015

Genetic characterization of Uruguayan Pampa Rocha pigs with microsatellite markers
Provedor de dados: 74
Autores: Montenegro,M; Llambí,S; Castro,G; Barlocco,N; Vadell,A; Landi,V; Delgado,JV; Martínez,A.
In this study, we genetically characterized the Uruguayan pig breed Pampa Rocha. Genetic variability was assessed by analyzing a panel of 25 microsatellite markers from a sample of 39 individuals. Pampa Rocha pigs showed high genetic variability with observed and expected heterozygosities of 0.583 and 0.603, respectively. The mean number of alleles was 5.72. Twenty-four markers were polymorphic, with 95.8% of them in Hardy Weinberg equilibrium. The level of endogamy was low (FIS = 0.0475). A factorial analysis of correspondence was used to assess the genetic differences between Pampa Rocha and other pig breeds; genetic distances were calculated, and a tree was designed to reflect the distance matrix. Individuals were also allocated into clusters. This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic resources; Microsatellites.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100048

Molecular characterization and genetic structure of the Nero Siciliano pig breed
Provedor de dados: 74
Autores: Guastella,Anna Maria; Criscione,Andrea; Marletta,Donata; Zuccaro,Antonio; Chies,Luigi; Bordonaro,Salvatore.
Nero Siciliano is an autochthonous pig breed that is reared mainly in semi-extensive systems in northeastern Sicily. Despite its economic importance and well-appreciated meat products, this breed is currently endangered. Consequently, an analysis of intra-breed variability is a fundamental step in preserving this genetic resource and its breeding system. In this work, we used 25 microsatellite markers to examine the genetic composition of 147 unrelated Nero Siciliano pigs. The total number of alleles detected (249, 9.96 per locus) and the expected heterozygosity (0.708) indicated that this breed had a high level of genetic variability. Bayesian cluster analysis showed that the most likely number of groups into which the sample could be partitioned was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cluster analysis; Coancestry; Intra-breed genetic variation; Microsatellites; Nero Siciliano pig breed.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400010

Developing a common bean core collection suitable for association mapping studies
Provedor de dados: 74
Autores: Perseguini,Juliana Morini Küpper Cardoso; Silva,Gliciane Micaele Borges; Rosa,João Ricardo Bachega Feijó; Gazaffi,Rodrigo; Marçal,Jéssica Fernanda; Carbonell,Sérgio Augusto Morais; Chiorato,Alisson Fernando; Zucchi,Maria Imaculada; Garcia,Antonio Augusto Franco; Benchimol-Reis,Luciana Lasry.
Because of the continuous introduction of germplasm from abroad, some collections have a high number of accessions, making it difficult to explore the genetic variability present in a germplasm bank for conservation and breeding purposes. Therefore, the aim of this study was to quantify and analyze the structure of genetic variability among 500 common bean accessions to construct a core collection. A total of 58 SSRs were used for this purpose. The polymorphism information content (PIC) in the 180 common bean accessions selected to compose the core collection ranged from 0.17 to 0.86, and the discriminatory power (DP) ranged from 0.21 to 0.90. The 500 accessions were clustered into 15 distinct groups and the 180 accessions into four distinct groups in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; Genetic diversity; Genetic structure; Microsatellites; Phaseolus vulgaris L.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100067

Frequency and distribution of microsatellites from ESTs of citrus
Provedor de dados: 74
Autores: Palmieri,Darío Abel; Novelli,Valdenice Moreira; Bastianel,Marinês; Cristofani-Yaly,Mariângela; Astúa-Monge,Gustavo; Carlos,Eduardo Fermino; Oliveira,Antonio Carlos de; Machado,Marcos Antonio.
Nearly 65,000 citrus EST (Expressed Sequence Tags) have been investigated using the CitEST project database. Microsatellites were investigated in the unigene sequences from Citrus spp. and Poncirus trifoliata. From these sequences, approximately 35% of the non-redundant ESTs contained SSRs. The frequencies of different SSR motifs were similar between Citrus spp and trifoliate orange. In general, mononucleotide repeats appeared to be the most abundant SSRs in the CitEST database, but we also identify di-, tri-, tetra-, penta- and hexanucleotide repeats. The AG/CT and AAG/CTT were the most common dinucleotide and trinucleotide motifs, with frequencies of 54.4% and 25.2%, respectively. Primer sequences flanking SSR motifs were successfully designed and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus spp.; Poncirus trifoliata; ESTs; Microsatellites; In silico polymorphism.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500029

Microsatellites in Portuguese autochthonous horse breeds: usefulness for parentage testing
Provedor de dados: 74
Autores: Luís,C.; Cothran,E.Gus; Oom,M.M.
The use of DNA technology for parentage testing is increasing every day. Most laboratories have improved their exclusion probabilities (PE) by the addition of DNA microsatellite loci to standard blood-typing results. The efficacy of each locus depends on the number of alleles detected and their frequencies in the breeds tested. Here we analyzed the usefulness of six microsatellites for routine parentage testing in three Portuguese autochthonous horse breeds: Lusitano, Sorraia and Garrano. The DNA loci analyzed - ASB2, HMS3, HMS7, HTG4, HTG10, and VHL20 - were chosen based upon the polymorphism detected in other breeds with Iberian horse influence. The estimated probability of exclusion of wrongly named parents (PE) was high, with values ranging from 88.5%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; DNA; Portuguese horse breeds; Parentage testing.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200003

Isolation of (GA)n microsatellite sequences and description of a predicted MADS-box sequence isolated from common bean (Phaseolus vulgaris L.)
Provedor de dados: 74
Autores: Yaish,M.W.F.; Pérez de la Vega,M.
The isolation of (GA)n microsatellites using a highly microsatellite-enriched library is described for the first time in common bean (Phaseolus vulgaris L.). A relatively simple and effective method to isolate DNA repeats from microsatellite-enriched libraries based on hybridization-capture of repeat regions using biotin-conjugated oligonucleotids and non-radioactive colony hybridization was carried out. PCR products from 200 to 800 bp were obtained and cloned. Of the 60 clones sequenced, 21 yielded (GA)n microsatellites with n values equal or higher than six. These (GA)n microsatellite-containing loci could be useful for further genetic mapping studies. A (GA)n microsatellite linked to a putative MADS-box gene was identified. This sequence, which...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris L.; Genetic markers; Microsatellites; MADS-box; Transcriptional factor.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300019

Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations
Provedor de dados: 74
Autores: Tavares,Mara Garcia; Pietrani,Nathalia Teixeira; Durvale,Maxwell de Castro; Resende,Helder Canto; Campos,Lucio Antonio de Oliveira.
Melipona quadrifasciata is a stingless bee widely found throughout the Brazilian territory, with two recognized subspecies, M. quadrifasciata anthidioides, that exhibits interrupted metasomal stripes, and M. quadrifasciata quadrifasciata, with continuous metasomal stripes. This study aimed to estimate the genetic variability of these subspecies. For this purpose, 127 colonies from 15 Brazilian localities were analyzed, using nine species-specific microsatellite primers. At these loci, the number of alleles ranged from three to 15 (mean: 7.2), and the observed heterozygosity (Ho) ranged from 0.03-0.21, while the expected heterozygosity (He) ranged from 0.23-0.47. The genetic distances among populations ranged from 0.03-0.45. The F ST multilocus value (0.23)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic differentiation; Microsatellites; Population genetics; Stingless bees.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100016

High genetic diversity among and within bitter manioc varieties cultivated in different soil types in Central Amazonia
Provedor de dados: 74
Autores: Alves-Pereira,Alessandro; Peroni,Nivaldo; Cavallari,Marcelo Mattos; Lemes,Maristerra R.; Zucchi,Maria Imaculada; Clement,Charles R.
Abstract Although manioc is well adapted to nutrient-poor Oxisols of Amazonia, ethnobotanical observations show that bitter manioc is also frequently cultivated in the highly fertile soils of the floodplains and Amazonian dark earths (ADE) along the middle Madeira River. Because different sets of varieties are grown in each soil type, and there are agronomic similarities between ADE and floodplain varieties, it was hypothesized that varieties grown in ADE and floodplain were more closely related to each other than either is to varieties grown in Oxisols. We tested this hypothesis evaluating the intra-varietal genetic diversity and the genetic relationships among manioc varieties commonly cultivated in Oxisols, ADE and floodplain soils. Genetic results did...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Microsatellites; Amazonian dark earths; Floodplain; Oxisols.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300468

Molecular markers for population genetic analyses in the family Psittacidae (Psittaciformes, Aves)
Provedor de dados: 74
Autores: Faria,Patrícia J.; Miyaki,Cristina Y.
The selection of molecular markers for population studies is an important tool for biodiversity conservation. The family Psittacidae contains many endangered and vulnerable species and we tested three kinds of molecular markers for their potential use in population studies of five psitacid species: 43 hyacinth macaws (Anodorhynchus hyacinthinus), 42 blue-and-yellow macaws (Ara ararauna), 23 red-and-green macaws (Ara chloroptera), 19 red-spectacled amazons (Amazona pretrei); and 18 red-tailed amazons (Amazona brasiliensis). We tested 21 clones from a genomic library of golden conure (Guarouba guarouba) minisatellites and 12 pairs of microsatellite primers developed for the domestic chicken (Gallus gallus) and A. hyacinthinus. We also tested seven...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Minisatellites; Molecular markers; Parrots; Population studies.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200007

Microsatellites for detecting inconsistencies in Capsicum cultivars registration in Brazilian database: more than meets the eye
Provedor de dados: 75
Autores: Azevedo,Carlos Diego de O; Rodrigues,Rosana; Sudré,Cláudia P.
ABSTRACT In Brazil, cultivars are registered by National Register of Cultivars (RNC), which besides enabling commercialization of cultivar propagative material, also guarantees the producers genetic purity and identity of propagules. However, it is possible that the information about registration and commercialization of some cultivars is inaccurate. This study aims to analyze the use of microsatellite markers to detect inconsistencies in data of Capsicum spp. cultivars obtained from the official database (CultivarWeb). Seven cultivars were evaluated, three of them were through genetic identity analysis (Amarela Comprida, De Cayenne and Cayenne Long Slin) and the others were used as standard for the species C. annuum, C. frutescens and C. chinense....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capsicum spp.; Microsatellites; Registered cultivars.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362019000300285

Molecular characterization of potato cultivars using SSR markers
Provedor de dados: 75
Autores: Favoretto,Patrícia; Veasey,Elizabeth Ann; Melo,Paulo César Tavares de.
The potato crop has a very narrow genetic base, so the use of molecular markers is a very important tool in the characterization of germplasm banks and evaluation of genetic divergence. The objective of this study was to identify, using microsatellite or simple sequence repeat (SSR) markers, 38 accessions of potato from two collections of commercial cultivars. For the molecular characterization 10 loci were used, generating a total of 46 alleles, which were analyzed as binary data. A cluster analysis was performed with the Jaccard´s similarity coefficient and the UPGMA method, using the software NTSYSpc. On average, the number of alleles per locus was 4.6, ranging from two alleles for primers STM1049, STM 1053 and STM 1104 to 12 alleles per locus for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum tuberosum; Germplasm banks; Genetic diversity; Microsatellites.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000400017

Genetic similarity between coriander genotypes using ISSR markers
Provedor de dados: 75
Autores: Melo,Roberto de A; Resende,Luciane V; Menezes,Dimas; Beck,Ana Paula A; Costa,José Carlos da; Coutinho,Alisson E; Nascimento,Ana Verônica S do.
With the development of new cultivars, a precise genetic characterization is essential for improvement programs or for cultivar registration and protection. Molecular markers have been complementing the traditional morphological and agronomic characterization techniques because they are virtually unlimited, cover the whole genome and are not environmentally influenced. Genetic characterization constitutes the basis for studies involving estimates of genetic similarity. Therefore, the objective of the present study was to evaluate the genetic similarity between ten coriander genotypes (nine cultivars and one line) using ISSR markers. The cultivars used were: Americano, Asteca, Palmeira, Português, Santo, Supéria, Tabocas, Tapacurá, Verdão and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coriandrum sativum L; Molecular characterization; Microsatellites; Condiments.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000400014

Identification de polymorphismes de gènes chez l'huître plate Ostrea edulis L. et variabilité au sein de populations européennes
Provedor de dados: 5
Autores: Chupeau, Gaelle.
Le but de ce travail était de rechercher des marqueurs non neutres afin de comparer ces données obtenues avec celles données par les marqueurs précédemment étudiés (marqueurs neutres ou supposés l'être à savoir microsatellites, enzymatique et mitochondriales). Dû à des soucis d'ADN extrait des populations qui avaient été étudiées jusqu'alors, il a fallu effectuer un nouvel échantillonnage de l'huître plate Européenne, Ostrea edulis, sur son aire de répartition. Nous avons donc analysé en plus du but majeur, les données microsatellites pour ces nouvelles populations de manière à avoir un jeu de données de marqueurs non neutres. Ces analyses ont ainsi complété l'arbre phylogénétique déjà existant, et ont confirmé dans une moindre mesure puisque tous les...
Tipo: Text
Palavras-chave: Huître plate; Ostrea edulis; Génétique des populations; Nucleotide Polymorphisme (SNP); Test de neutralisté; Microsatellites.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/00033/14417/11712.pdf

Caractérisation génétique de l’effort reproducteur de l’huître creuse, Crassostrea gigas, dans le cadre des mortalités estivales de juvéniles : Approch QTL
Provedor de dados: 5
Autores: Flahauw, Emilie.
The Pacific oyster, Crassostrea gigas, is a major aquacultured species whose production represents an economic interest at worldwide, european and french levels. However, this species undergoes summer mortalities recorded from the beginning of the 20th century and, since 2008, this phenomenon increased and threatens mainly juvenile oysters. Aquaculture production of oysters suffers consequences of mass mortalities, that’s why this phenomenon has been studied for many years. In France, the bacterium Vibrio splendidus and the Ostreid virus Herpes Virus 1 (OsHV-1) are often associated with mass mortality outbreaks of juveniles oysters and it was demonstrated that selected individuals for resistance to summer mortality were able to slow the increasing in viral...
Tipo: Text
Palavras-chave: Huître creuse du Pacifique; Crassostrea gigas; Mortalités estivales; Survie; Effort reproducteur; Imagerie par Résonance Magnétique; Histologie; Marqueurs microsatellites; Marqueurs SNP; Cartographie de liaison; Détection de QTL; Pacific oyster; Crassostrea gigas; Summer mortality; Survival; Reproductive effort; Magnetif Resonance Imaging; Histology; Microsatellites; SNPs; Linkage mapping; QTL detection.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00233/34377/33786.pdf

The use of microsatellite markers for parentage analysis in the Pacific cupped oyster, Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: Boudry, Pierre; Collet, Bertrand; Kotoulas, Georgios; Magoulas, Antonio; Hervouet, Veronique; Bonhomme, Francois; Gerard, Andre.
Highly polymorphic genetic markers can be useful tools for the analysis of parental contributions. The present paper reports 3 experiments using microsatellite markers to analyse parental contributions in controlled in vitro factorial crosses of the cupped oyster Crassostrea gigas. Parentage analysis was eased by the large polymorphism observed at the 3 loci studied. In a cross between 20 males and 20 females, a quarter of the progeny could be assigned to a given parent using a single microsatellite locus showing 28 alleles. In 3 crosses between 5 males and females, all the progeny could be assigned to a family by using 3 loci. In all cases, the combined genotyping at 2 loci allowed parentage to be detennined unambiguously. Despite the balanced gametic...
Tipo: Text
Palavras-chave: Crassostrea gigas; Microsatellites; Parental contributions; Crassostrea gigas; Microsatellites; Contributions parentales.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/1998/acte-2739.pdf

Response to domestication and selection for growth in the European sea bass (Dicentrarchus labrax) in separate and mixed tanks
Provedor de dados: 5
Autores: Vandeputte, Marc; Dupont Nivet, M; Haffray, P; Chavanne, H; Cenadelli, S; Parati, K; Vidal, Marie-odile; Vergnet, Alain; Chatain, Beatrice.
Selective breeding of European sea bass (Dicentrarchus labrax) receives a growing interest, as the estimated heritability of growth is medium to high. In this study, we compared the offspring of four groups of sea bass sires, mated with the same wild dams: wild (W), first generation of domestication (D), first generation of mass selection for length (M), first generation of PROSPER-like selection for length (P). The comparison was done both in replicated tanks (separate rearing) and in mixed tanks (mixed rearing) where sire origins were recovered by genotyping of eight microsatellite markers. Weight, length and growth rate were measured from day 238 post-fertilization (69 g mean weight) to day 611 post-fertilization (390 g mean weight). Both in mixed and...
Tipo: Text
Palavras-chave: Nephrocalcinosis; Competition; Microsatellites; Parentage assignment; Realized heritability; Selection response; Growth; Selective breeding; Dicentrarchus labrax; European sea bass.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-5303.pdf

Domestication en phase larvaire chez l'huître creuse Crassostrea gigas. Effets sélectifs des conditions environnementales appliquées en écloseries sur la diversité génétique
Provedor de dados: 5
Autores: Sauvage, Christopher.
L'huître creuse Crassostrea gigas est, aujourd'hui, la première espèce ostréicole française avec une production de 140000 tonnes par an. Parallèlement au recrutement naturel, les écloseries produisent aujourd'hui 20% du naissain mis en élevage. Cette production est réalisée dans des conditions assurant une productivité maximale (température supérieure à 25°C, ration alimentaire optimale et tamisage sélectif). Peu de travaux se sont intéressés à l'impact génétique de ces pratiques d'élevage intensif au stade larvaire. Notre étude porte sur l'effet de la température appliquée lors de l'élevage larvaire sur la croissance et la survie et leurs composantes génétiques. Un croisement factoriel de 4 femelles et 12 mâles a été réalisé, en choisissant les géniteurs...
Tipo: Text
Palavras-chave: Crassostrea gigas; Ecloseries; Croissance; Survie; Température; Microsatellites; Larves; Sélection.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/00033/14418/11713.pdf

Genetic population structure of the Swordfish (Xiphias gladius) in the southwest Indian Ocean: Sex-biased differentiation, congruency between markers and its incidence in a way of stock assessment
Provedor de dados: 5
Autores: Muths, Delphine; Grewe, R.; Jean, Claire; Bourjea, Jerome.
Genetic variation was surveyed at 11 microsatellite loci and at 517 bp of the mitochondrial control region to investigate the presence of genetic stock structure in swordfish (Xiphias gladius) in four proximal localities of the southwest Indian Ocean. One aim of this study was to serve as a preliminary examination for congruency of structure detected by these two genetic markers, prior to conducting a more comprehensive basin-wide survey of the Indian Ocean and nearby surrounding areas. Analyses of multilocus microsatellite genotypes and mitochondrial control region sequences both revealed a great homogeneity between samples. Genetic diversity detected at the regional scale was not significantly higher than detected at the local scale. Results suggest that...
Tipo: Text
Palavras-chave: Indian Ocean; Population genetic; MtDNA; Microsatellites; Swordfish.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6455.pdf

Disentangling the Influence of Mutation and Migration in Clonal Seagrasses Using the Genetic Diversity Spectrum for Microsatellites
Provedor de dados: 5
Autores: Arnaud-haond, Sophie; Moalic, Yann; Hernandez-garcia, Emilio; Eguiluz, Victor M.; Alberto, Filipe; Serrao, Ester A.; Duarte, Carlos M.
The recurrent lack of isolation by distance reported at regional scale in seagrass species was recently suggested to stem from stochastic events of large-scale dispersal. We explored the usefulness of phylogenetic information contained in microsatellite loci to test this hypothesis by using the Genetic Diversity Spectrum (GDS) on databases containing, respectively, 7 and 9 microsatellites genotypes for 1541 sampling units of Posidonia oceanica and 1647 of Cymodocea nodosa. The simultaneous increase of microsatellite and geographic distances that emerges reveals a coherent pattern of isolation by distance in contrast to the chaotic pattern previously described using allele frequencies, in particular, for the long-lived P. oceanica. These results suggest...
Tipo: Text
Palavras-chave: Clonality; Genetic Diversity Spectrum; Genetic divergence; Microsatellites; Seagrass; Stepwise mutation.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00182/29292/27733.pdf

Diversité et différentiation génétiques des populations de tortues vertes (Chelonia mydas) dans les sites de ponte et d'alimentation du sud-ouest de l'océan Indien : application aux stratégies de conservation de l'espèce
Provedor de dados: 5
Autores: Taquet, Coralie.
The green turtle (Chelonia mydas) is an emblematic species of marine life. However, nowadays it is subject to many threats (poaching, by-catch). Even if there is deep growing measures for its protection, the green turtle still is an endangered species and it is listed in Appendix I of Washington Convention (CITES). In order to elaborate efficient conservation and management plans, perfect knowledge of green turtle biology, but also of its population structure and their characteristics, are needed.  In this thesis, we have assessed genetic structure of green turtle populations in the South-Western Indian Ocean by using genetic tools. In all, 1551 tissue samples have been collected from our study zone and from our control site French Polynesia (37 samples)....
Tipo: Text
Palavras-chave: Management units; Microsatellites; Mitochondrial DNA; Foraging sites; Nesting sites; South Western Indian Ocean; Population genetics; Chelonia mydas; Green turtle; Unités de gestion (MUs); Microsatellites; ADN mitochondrial; Sites d'alimentation; Sites de ponte; Génétique des populations; Chelonia mydas; Tortue verte.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/these-3532.pdf

Genetic variability and selective breeding for traits of aquacultural interest in the Pacific oyster (Crassostrea gigas)
Provedor de dados: 5
Autores: Boudry, Pierre; Degremont, Lionel; Taris, Nicolas; Mccombie, Helen; Haffray, Pierrick; Ernande, Bruno.
The most significant genetic improvement for production of Pacific oyster (Crassastrea gigas) has been obtained through the breeding of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or for other traits of aquacultural interest, could be obtained using this approach. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have slowed the development of selective breeding programs. Recently, in the USA, Australia and New Zealand, family-based selective breeding programs have been initiated to improve growth and yield. In Europe, where both natural and...
Tipo: Text
Palavras-chave: Microsatellites; Quantitative genetic; Genetic variability; Crassostrea gigas; Oysters.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-2861.pdf

Role of evolutionary and ecological factors in the reproductive success and the spatial genetic structure of the temperate gorgonian Paramuricea clavata
Provedor de dados: 5
Autores: Mokhtar-jamai, Kenza; Coma, Rafel; Wang, Jinliang; Zuberer, Frederic; Feral, Jean-pierre; Aurelle, Didier.
Dispersal and mating features strongly influence the evolutionary dynamics and the spatial genetic structure (SGS) of marine populations. For the first time in a marine invertebrate, we examined individual reproductive success, by conducting larval paternity assignments after a natural spawning event, combined with a small-scale SGS analysis within a population of the gorgonian Paramuricea clavata. Thirty four percent of the larvae were sired by male colonies surrounding the brooding female colonies, revealing that the bulk of the mating was accomplished by males from outside the studied area. Male success increased with male height and decreased with increasing male to female distance. The parentage analyses, with a strong level of self-recruitment (25%),...
Tipo: Text
Palavras-chave: Gamete and larval dispersal; Mating system; Microsatellites; Natural spawning event; Parentage analyses.
Ano: 2013
URL: https://archimer.ifremer.fr/doc/00372/48281/48502.pdf

Genetic diversity of the European oyster (Ostrea edulis L.) in Nova Scotia: Comparison with other parts of Canada, Maine and Europe and implications for broodstock management
Provedor de dados: 5
Autores: Vercaemer, Bénédikte; Spence, Koren R.; Herbinger, Christophe; Lapegue, Sylvie; Kenchington, Ellen L.
The European oyster (Ostrea edulis) was introduced to the Nova Scotia aquaculture industry 30 years ago using stocks imported from naturalized populations in Maine whose ancestors originated in the Netherlands. This study used 5 microsatellites to assess the level of genetic diversity in several hatchery stocks and naturalized populations from Nova Scotia, New Brunswick, British Columbia and Maine. Some genetic erosion was shown to have occurred in the Maritimes populations, with the largest loss of alleles being experienced by the hatchery stocks. In spite of this loss, genetic diversity and heterozygosity in the Maritimes populations are still relatively high. Relationships within and between the populations and the existence of kin groups within the...
Tipo: Text
Palavras-chave: Genetic diversity; Maine; Microsatellites; Flat oyster; European oyster; Ostrea edulis.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2121.pdf

Heritabilities and GxE interactions for growth in the European sea bass (Dicentrarchus labrax L.) using a marker-based pedigree
Provedor de dados: 5
Autores: Dupont Nivet, M; Vandeputte, Marc; Vergnet, Alain; Merdy, O; Haffray, P; Chavanne, H; Chatain, Beatrice.
253 full-sib families from 33 males and 23 females of European seabass were produced in a partly factorial mating design. All fish were reared in the same tank during 14 months, then 7000 of them were dispatched in four farms to different locations (France, Israel, Italy, Portugal) representing a wide variety of environmental conditions. Around 400 g mean weight, 1177 to 1667 fish in each site were weighed and length was measured. Condition factor (K) was calculated. Pedigrees were redrawn a posteriori using microsatellites markers: parental origin could be retraced for 99.2% of fish. Due to a high incidence of deformities, the useful sample size was reduced to 491-670 fish per site.   Maternal effects were small. Using a simple animal model, heritability...
Tipo: Text
Palavras-chave: Microsatellites; GxE interactions; Heritability; Growth; Seabass; Dicentrarchus labrax.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4404.pdf

From population connectivity to the art of striping Russian dolls: the lessons from     Pocillopora     corals
Provedor de dados: 5
Autores: Gelin, Pauline; Fauvelot, Cecile; Bigot, Lionel; Baly, Joseph; Magalon, Helene.
Here, we examined the genetic variability in the coral genus Pocillopora, in particular within the Primary Species Hypothesis PSH09, identified by Gélin, Postaire, Fauvelot and Magalon (2017) using species delimitation methods [also named Pocillopora eydouxi/meandrina complex sensu, Schmidt-Roach, Miller, Lundgren, &amp; Andreakis (2014)] and which was found to split into three secondary species hypotheses (SSH09a, SSH09b, and SSH09c) according to assignment tests using multi-locus genotypes (13 microsatellites). From a large sampling (2,507 colonies) achieved in three marine provinces [Western Indian Ocean (WIO), Tropical Southwestern Pacific (TSP), and Southeast Polynesia (SEP)], genetic structuring analysis conducted with two clustering analyses...
Tipo: Text
Palavras-chave: Bayesian assignments; Cluster; DAPC; Microsatellites; Pocillopora; Scleractinian.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00416/52776/53649.pdf

Origin and route of establishment of the invasive Pacific oyster Crassostrea gigas in Scandinavia
Provedor de dados: 5
Autores: Faust, Ellika; Andre, Carl; Meurling, Sara; Kochmann, Judith; Christiansen, Henrik; Jensen, Lasse Fast; Charrier, Gregory; Laugen, Ane T.; Strand, Asa.
Identifying the routes and rates of introductions is fundamental for the understanding of marine invasions. Recurring introductions over the last 50 yr have led to the establishment of feral Pacific oyster Crassostrea gigas populations throughout Europe. In the northern countries, Sweden and Norway, the species first occurred in large numbers in 2006. Here, we investigated the relative importance of introduction via re-laying of cultured oysters imported for consumption from France, Ireland or the Netherlands, and dispersal of oyster larvae by ocean currents from wild oyster populations in Denmark. Using microsatellite DNA markers, we estimated genetic differentiation among Pacific oysters collected at 4 Swedish locations, 3 Norwegian locations and 9...
Tipo: Text
Palavras-chave: Population genetics; Microsatellites; Range expansion; Non-native species; Aquaculture; Connectivity; Scandinavia; Skagerrak.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00395/50627/72574.pdf

Genetic structure of a commercially exploited bivalve, the great scallop Pecten maximus, along the European coasts
Provedor de dados: 5
Autores: Morvezen, Romain; Charrier, Gregory; Boudry, Pierre; Chauvaud, Laurent; Breton, Florian; Strand, Oivind; Laroche, Jean.
The great scallop Pecten maximus is harvested in several European countries and fisheries targeting this species are severely regulated by fishing quotas. In addition, hatchery-based population enhancement has been developed in some countries to provide alternative or complementary production. The genetic structure of wild populations of P. maximus and the potential impact of aquaculture on the genetic diversity of this species remains poorly documented. In this study, we explored the genetic structure of P. maximus using 12 microsatellite markers, considering 14 populations sampled from Galicia (Spain) to the North of Norway, and one population of Pecten jacobaeus (L., 1758) from the Lion Gulf (Mediterranean Sea). Results indicated a clear differentiation...
Tipo: Text
Palavras-chave: Great scallop; Pecten maximus; Microsatellites; Population genetics; Aquaculture.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00311/42251/41692.pdf

Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles inferred from microsatellite and mitochondrial markers
Provedor de dados: 5
Autores: Lallias, Delphine; Boudry, Pierre; Batista, Frederico; Beaumont, Andy; King, Jonathan W.; Turner, John R.; Lapegue, Sylvie.
The Pacific oyster, Crassostrea gigas, native to northeast Asia, is one of the most important cultured shellfish species. In Europe, Pacific oysters first settled along the Atlantic coasts of France at the end of the 1960s but rapidly spread and are now widely established. Twenty-two sites in the United Kingdom (UK), Ireland, Denmark, France and Spain were sampled to assess genetic diversity and differentiation. Hatchery-propagated stocks from two hatcheries located in the UK also were included. Two main genetic clusters were identified from pairwise genetic differentiation indexes, Bayesian clustering methods or neighbour-joining analysis, based on 7 microsatellite loci: (1) a Northeast cluster (including feral samples from East England, Ireland and...
Tipo: Text
Palavras-chave: Invasion genetics; Marine mollusc; Microsatellites; MtDNA; Pacific oyster; Crassostrea gigas.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00268/37973/36052.pdf

Isolation and characterization of eight microsatellite loci from Galeocerdo cuvier (tiger shark) and cross-amplification in Carcharhinus leucas, Carcharhinus brevipinna, Carcharhinus plumbeus and Sphyrna lewini
Provedor de dados: 5
Autores: Pirog, Agathe; Jaquemet, Sebastien; Blaison, Antonin; Soria, Marc; Magalon, Helene.
The tiger shark Galeocerdo cuvier (Carcharhinidae) is a large elasmobranch suspected to have, as other apex predators, a keystone function in marine ecosystems and is currently considered Near Threatened (Red list IUCN). Knowledge on its ecology, which is crucial to design proper conservation and management plans, is very scarce. Here we describe the isolation of eight polymorphic microsatellite loci using 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries. Their characteristics were tested on a population of tiger shark (n = 101) from Reunion Island (South-Western Indian Ocean). All loci were polymorphic with a number of alleles ranging from two to eight. No null alleles were detected and no linkage disequilibrium was detected after Bonferroni...
Tipo: Text
Palavras-chave: Carcharhiniform; Microsatellites; Control region; Population Genetics.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00626/73769/74405.pdf

Characterization of ten microsatellite loci in the blue mussel Mytilus edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Stockdale, Ruth; Boudry, Pierre; Lapegue, Sylvie; Beaumont, Andy R.
Mussels of the genus Mytilus are ecologically and commercially important worldwide, and they form hybrid complexes where their distributions overlap. Allozyme and nuclear markers have bee It Used to investigate their genetics over many years, but successful development of reliable highly valuable microsatellite markers has lagged be hind other shellfish species. We have developed and characterized tell novel microsatellite loci that amplify reliably for the blue mussel Mytilus edulis. The number of alleles among 30 individuals from a wild population (Menai Strait, North Wales UK) ranged between 9 and 29 and the observed heterozygosity between 0.300 and 0.954. Significant heterozygote deficiencies against the Hardy-Weinberg model were observed at 6 out of...
Tipo: Text
Palavras-chave: Mytilus edulis; Microsatellites; Universal tailed-primer labeling; Null alleles; Mussels.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00002/11275/7841.pdf

Characterization of 27 microsatellite loci in the European flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Stockdale, Ruth; Boudry, Pierre; Beaumont, Andy R.; Lapegue, Sylvie.
The flat oyster Ostrea edulis is native to Europe and populations have been severely depleted by the parasite Bonamia ostreae since the 1980s. Additional genetic markers are required to improve population genetics study and linkage map development for selection for B. ostrea-resistance in this species. Here, we characterized 27 novel microsatellite loci for O. edulis. Number of alleles per locus ranged from 6 to 25 and observed heterozygosity between 0.375 and 1. Null alleles were suggested at a few loci but most loci were in Hardy-Weinberg agreement enabling their reliable use in further population and mapping genetics approaches.
Tipo: Text
Palavras-chave: Universal tailed primer labelling; Oysters; Ostrea edulis; Microsatellites.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00341/45195/44592.pdf

Population differentiation or species formation across the Indian and the Pacific Oceans? An example from the brooding marine hydrozoan Macrorhynchia phoenicea
Provedor de dados: 5
Autores: Postaire, Bautisse; Gelin, Pauline; Bruggemann, J. Henrich; Pratlong, Marine; Magalon, Helene.
Assessing population connectivity is necessary to construct effective marine protected areas. This connectivity depends, among other parameters, inherently on species dispersal capacities. Isolation by distance (IBD) is one of the main modes of differentiation in marine species, above all in species presenting low dispersal abilities. This study reports the genetic structuring in the tropical hydrozoan Macrorhynchia phoenicea α (sensu Postaire et al., 2016a), a brooding species, from 30 sampling sites in the Western Indian Ocean and the Tropical Southwestern Pacific, using 15 microsatellite loci. At the local scale, genet dispersal relied on asexual propagation at short distance, which was not found at larger scales. Considering one representative per...
Tipo: Text
Palavras-chave: Assignment test; Brooding species; Cryptic diversity; Hydrozoa; Microsatellites.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00399/51011/51795.pdf

Detecting past changes of effective population size
Provedor de dados: 5
Autores: Nikolic, Natacha; Chevalet, Claude.
Understanding and predicting population abundance is a major challenge confronting scientists. Several genetic models have been developed using microsatellite markers to estimate the present and ancestral effective population sizes. However, to get an overview on the evolution of population requires that past fluctuation of population size be traceable. To address the question, we developed a new model estimating the past changes of effective population size from microsatellite by resolving coalescence theory and using approximate likelihoods in a Monte Carlo Markov Chain approach. The efficiency of the model and its sensitivity to gene flow and to assumptions on the mutational process were checked using simulated data and analysis. The model was found...
Tipo: Text
Palavras-chave: Atlantic salmon; Effective population size; Microsatellites; Past demography.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00194/30565/28986.pdf

Merging scleractinian genera: the overwhelming genetic similarity between solitary Desmophyllum and colonial Lophelia
Provedor de dados: 5
Autores: Addamo, Anna Maria; Vertino, Agostina; Stolarski, Jaroslaw; Garcia-jimenez, Ricardo; Taviani, Marco; Machordom, Annie.
Background  In recent years, several types of molecular markers and new microscale skeletal characters have shown potential as powerful tools for phylogenetic reconstructions and higher-level taxonomy of scleractinian corals. Nonetheless, discrimination of closely related taxa is still highly controversial in scleractinian coral research. Here we used newly sequenced complete mitochondrial genomes and 30 microsatellites to define the genetic divergence between two closely related azooxanthellate taxa of the family Caryophylliidae: solitary Desmophyllum dianthus and colonial Lophelia pertusa. Results  In the mitochondrial control region, an astonishing 99.8 % of nucleotides between L. pertusa and D. dianthus were identical. Variability of the mitochondrial...
Tipo: Text
Palavras-chave: Mitochondrial genome; Microsatellites; Genetic divergence; Skeletal plasticity; Desmophyllum dianthus; Lophelia pertusa.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00334/44541/44248.pdf

APIS: An Auto‐Adaptive Parentage Inference Software that tolerates missing parents
Provedor de dados: 5
Autores: Griot, Ronan; Allal, Francois; Brard‐fudulea, S; Morvezen, R; Haffray, P; Phocas, F; Vandeputte, Marc.
In the context of parentage assignment using genomic markers, key issues are genotyping errors and an absence of parent genotypes because of sampling, traceability or genotyping problems. Most likelihood‐based parentage assignment software programs require a priori estimates of genotyping errors and the proportion of missing parents to set up meaningful assignment decision rules. We present here the R package APIS, which can assign offspring to their parents without any prior information other than the offspring and parental genotypes, and a user‐defined, acceptable error rate among assigned offspring. Assignment decision rules use the distributions of average Mendelian transmission probabilities, which enable estimates of the proportion of offspring with...
Tipo: Text
Palavras-chave: Microsatellites; Missing parents; Parentage assignment; Pedigree; SNP.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00587/69866/67767.pdf

Variable microsatellites in the Pacific Oyster Crassostrea gigas and other cupped oyster species
Provedor de dados: 5
Autores: Huvet, Arnaud; Boudry, Pierre; Ohresser, M; Delsert, Claude; Bonhomme, F.
Source/description : Genomic DNA was extracted from a whole Pacific oyster (C. gigas) after grinding in liquid nitrogen. Purified DNA was then digested by a mix of 3 restriction enzymes (AluI, RsaI, HaeIII). DNA fragments ranging from 250 to 500 bp were size-selected by agarose gel electrophoresis and ligated into the dephosphorylated blunt-ended SmaI-linearised pBKS2 plasmid (Stratagene Cloning System, CA). White colonies (11,500) were screened by hybridisation with double-stranded DNA probes containing dinucleotide repeats. Poly(dAdC), poly(dAdG), poly(dAdT) (Pharmacia Biotech, Sweden) were mixed at equimolar ratio and radiolabelled by [&amp;#61537;-32P] alpha dATP and dCTP using a random priming labelling kit (Life Technologies, Germany). Filter...
Tipo: Text
Palavras-chave: Crassostrea gigas; Oyster; Microsatellites.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-703.pdf

Différenciation génétique de deux huîtres creuses Crassostrea gigas et Crassostrea angulata : apport des marqueurs microsatellites
Provedor de dados: 5
Autores: Huvet, Arnaud.
Les définitions taxonomiques de Crassostrea angulata et Crassostrea gigas font l'objet de controverses. Les similitudes morphologiques et physiologiques ainsi que l'homogénéité des fréquences allozymiques entre populations des deux taxons orientent les auteurs vers un regroupement au sein d'une seule espèce. Récemment, une étude basée sur l'analyse par RFLP de l'ADN mitochondrial a révélé des différences permettant de distinguer C. angulata et C. gigas. La caractérisation par 4 marqueurs microsatellites de 12 populations des 2 taxons révèlent les points suivants : des déficits en hétérozygotes sont observés dans l'ensemble des populations, l'analyse de la variabilité inter-populations montre l'existence d'une structuration, l'analyse des distances...
Tipo: Text
Palavras-chave: Crassostrea gigas; Crassostrea angulata; Microsatellites; Différenciation.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00033/14377/11667.pdf

Population genomics shed light on the demographic and adaptive histories of European invasion in the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Cornette, Florence; Lapegue, Sylvie.
Crassostrea gigas originated from the Pacific coast of Asia, but was introduced into several European countries in the early 1970s. Natural populations have now spread across the length of the western seaboard of Europe. To elucidate the demographic and selective processes at play during this rapid expansion, genome-scan analysis was performed on different populations. High diversities and low differentiation were observed overall, but significant genetic differentiation was found among newly established populations and between the newly established northern group and a nearly panmictic group composed of southern European populations and a population from Japan. Loss of genetic diversity was also seen in the north, likely caused by founder events during...
Tipo: Text
Palavras-chave: AFLPs; Crassostrea gigas; Genome scan; Invasive species; Microsatellites; SNPs.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00149/25977/24070.pdf

Microsatellite analysis of 6-hour-old embryos reveals no preferential intraspecific fertilization between cupped oysters Crassostrea gigas and Crassostrea angulata
Provedor de dados: 5
Autores: Huvet, Arnaud; Balabaud, K; Bierne, Nicolas; Boudry, Pierre.
Experimental examination of reproductive isolation is the first step in understanding li,hybridization processes. Here, we studied preferential fertilization between 2 cupped oyster taxa, Crassostrea angulata and Crassostrea gigas, as a potential prezygotic reproductive isolation. Early examination of sperm competition is now possible by molecular analysis of oyster embryos. This avoids the confounding effect of differential mortality during the larval stage. Six hundred embryos were sampled from 2 crosses. Three microsatellite loci were enough to determine without ambiguity the taxa of contributing sires of embryos. No evidence of preferential fertilization between gametes from the same taxa was shown. A significantly higher contribution of the C. gigas...
Tipo: Text
Palavras-chave: Paternal contribution; Microsatellites; In vitro crossings; Hybridization; Crassostrea angulata; Crassostrea gigas.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-436.pdf

Microsatellite-based parentage analysis of factorial crosses in Pacific oyster (Crassostrea gigas) larvae and spat
Provedor de dados: 5
Autores: Taris, Nicolas; Sauvage, Christopher; Ernande, Bruno; Boudry, Pierre.
Several studies have suggested that significant genetic variability might exist for some early developmental traits in the Pacific oyster, C. gigas. However, most studies have examined only limited numbers of families.  More critically, these families were rarely replicated, leading to possible bias due to heterogeneous larval rearing conditions among families. To overcome these potential problems, we used a mixed-family approach with subsequent parentage analysis. A recently developed set of three multiplexed microsatellite markers was used to determine parentage in a complete factorial cross between 12 males and 4 females. Larvae were reared at two different temperatures (20 and 26°C) to provide a contrast resembling wild versus hatchery conditions....
Tipo: Text
Palavras-chave: Selection; Genetic variability; Microsatellites; Crassostrea gigas; Pacific oysters.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3440.pdf

From theory to practice: Empirical evaluation of the assignment power of marker sets for pedigree analysis in fish breeding
Provedor de dados: 5
Autores: Vandeputte, Marc; Rossignol, Marie-noelle; Pincent, Cedric.
The use of marker-based pedigrees is increasing in aquaculture breeding, and obtaining high assignment rates is necessary for practical use of this methodology. In this paper, we used 12 real parentage assignment datasets from three species (European sea bass Dicentrarchus labrax, common carp Cyprinus carpio and rainbow trout Oncorhynchus mykiss) to investigate the relationships between theoretical, simulated and real assignment power. We found out that there was a large decrease between the theoretical and the observed values, which we modeled in four independent steps: 1) from theoretical values to population-wise simulations (-2.8% on average), 2) from population-wise simulations to true parent set specific simulations (-2.6% on average), 3) from true...
Tipo: Text
Palavras-chave: Aquaculture; Parentage assignment; Exclusion probability; Microsatellites.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00035/14586/11964.pdf

Maintenance of genetic differentiation across a transition zone in the sea: discordance between nuclear and cytoplasmic markers
Provedor de dados: 5
Autores: Lemaire, Christophe; Versini, J; Bonhomme, Francois.
To investigate the origin and maintenance of the genetic discontinuity between Atlantic and Mediterranean populations of the common sea bass (Dicentrarchus labrax) we analysed the genetic variation at a fragment of mitochondrial cytochrome b sequence for 18 population samples. The result were also compared with new or previously published microsatellite data. Seven mitochondrial haplotypes and an average nucleotidic divergence of 0.02 between Atlantic and Mediterranean populations that matches a Pleistocene allopatric isolation were found. The frequency variation at the cytochrome b locus was many times greater between Atlantic and Mediterranean populations ((&amp;#952;) over cap (C) = 0.67) than at microsatellite loci ((&amp;#952;) over cap (N) = 0.02)....
Tipo: Text
Palavras-chave: Reproductive isolation; Phylogeography; MtDNA; Microsatellites; Genetic transitions.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-776.pdf

Mitochondrial and nuclear DNA phylogeography of Crassostrea angulata, the Portuguese oyster endangered in Europe
Provedor de dados: 5
Autores: Huvet, Arnaud; Lapegue, Sylvie; Magoulas, A; Boudry, Pierre.
The respective status of the Portuguese oyster, Crassostrea angulata, and the Pacific oyster, Crassostrea gigas, has long been a matter of controversy. Morphological and physiological similarities, homogeneity of allozyme allelic frequencies between populations of the two taxa and the demonstration of hybridization lead most authors to suggest that they should be regrouped within the same species. The risk of introgression and the present expansion of C. gigas aquaculture in Europe raises the question of the need for preservation of C. angulata in Europe, as only a few populations remain. We studied European and Asian populations of C. gigas and C. angulata using microsatellite and mitochondrial DNA markers to estimate their genetic diversity and...
Tipo: Text
Palavras-chave: Phylogeography; Population genetics; Microsatellites; Crassostrea gigas; Crassostrea angulata.
Ano: 2000
URL: https://archimer.ifremer.fr/doc/2000/publication-707.pdf

Molecular species delimitation methods and population genetics data reveal extensive lineage diversity and cryptic species in Aglaopheniidae (Hydrozoa)
Provedor de dados: 5
Autores: Postaire, Bautisse; Magalon, Helene; Bourmaud, Chloe A. -f.; Bruggemann, J. Henrich.
A comprehensive inventory of global biodiversity would be greatly improved by automating methods for species delimitation. The Automatic Barcode Gap Discovery method, the Poisson tree processes algorithm and the Generalized mixed Yule-coalescent model have been proposed as means of increasing the rate of biodiversity description using single locus data. We applied these methods to explore the diversity within the Aglaopheniidae, a hydrozoan family with many species widely distributed across tropical and temperate oceans. Our analyses revealed widespread cryptic diversity in this family, almost half of the morpho-species presenting several independent evolutionary lineages, as well as support for cases of synonymy. For two common species of this family,...
Tipo: Text
Palavras-chave: Automatic Barcode Gap Discovery; Generalized mixed Yule-coalescent model; Poisson tree processes; 16S; Microsatellites; Cnidarians.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00348/45907/45590.pdf

Detection and description of a particular Ostreid herpesvirus 1 genotype associated with massive mortality outbreaks of Pacific oysters, Crassostrea gigas, in France in 2008
Provedor de dados: 5
Autores: Segarra, Amelie; Pepin, Jean-francois; Arzul, Isabelle; Morga, Benjamin; Faury, Nicole; Renault, Tristan.
Ostreid herpesvirus 1 (OsHV-1) infections have been reported around the world and are associated with high mortalities of the Pacific oyster (Crassostrea gigas). In the summer 2008, abnormal mortality rates ranging from 80% to 100% were reported in France and affected only Pacific oysters. Analyses of oyster samples collected during mortality outbreaks demonstrated a significant detection of OsHV-1 (75% of analysed batches), which appeared stronger than previous years. DNA sequencing based on C and IA regions was carried out on 28 batches of OsHV-1 infected Pacific oysters collected in 2008. Polymorphisms were described in both the C and IA regions and characterized a genotype of OsHV-1 not already reported and termed OsHV-1 μVar. A microsatellite zone...
Tipo: Text
Palavras-chave: Herpes virus; Oyster; Crassostrea gigas; OsHV-1 mu Var; Increased mortality; Microsatellites.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00011/12260/9035.pdf

Genetic variation for growth at one and two summers of age in the common carp (Cyprinus carpio L.): Heritability estimates and response to selection
Provedor de dados: 5
Autores: Vandeputte, Marc; Kocour, M; Mauger, S; Rodina, M; Launay, A; Gela, D; Dupont Nivet, M; Hulak, M; Linhart, O.
We estimated the heritability of growth-related traits (weight and length at ages one summer, first spring and two summers) in a synthetic mirror carp strain (HSM) in the Czech Republic. The four generation pedigree was obtained from parentage assignment of three factorial mating designs with microsatellite markers, and included 195 fish without phenotypes (48 G0, 147 G1) and 1321 fish with phenotypes (674 G2, 647 G3). Animal model heritability estimates over generations were in the range 0.21-0.33 for length and in the range 0.31-0.44 for weight. The genetic correlation between length and weight was high (0.97). The correlations between growth measurements in the first and in the second summer of age were moderate to low (0.34-0.67). Divergent selection...
Tipo: Text
Palavras-chave: Quantitative genetics; Performance; Microsatellites; Parentage assignment; Realized heritability; Selection response; Selection; Growth; Heritability; Cyprinus carpio; Common carp.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4076.pdf

Utilisation des marqueurs microsatellites pour l'étude des contributions parentales chez l'huître creuse Crassostrea gigas
Provedor de dados: 5
Autores: Boudry, Pierre; Collet, Bertrand; Kotoulas, G.; Hervouet, Veronique; Cornette, Florence; Bonhomme, Francois; Gerard, Andre.
Numerous aquacultural species show very high fertilities. As a consequence, it is possible to produce, in hatcheries, a large amount of sibs from a small amount of parents. The sibs' genetic variability is, of course, directly linked to the number of parents involved, but it may be largely lowered by imbalanced parental contributions. Polymorphic genetic markers are very handy tools in the study of these phenomena in cultivated populations (Harris et al. 1991; Herbinger et al., 1996a; Garcia de Leon et al. 1998) or in natural populations (Ferguson et al, 1995; Kellog et al., 1995; Colbourne et al., 1996; Herbinger et al., 1996b). This document is a presentation of the results of a number of experiments, realised in the scope of the European program...
Tipo: Text
Palavras-chave: Microsatellites; Contributions parentales; Crassostrea gigas; Huîtres.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/acte-3452.pdf

Analyse du succès reproducteurs chez des populations naturelles et d'écloserie d'huître plate Ostrea edulis L.
Provedor de dados: 5
Autores: Lallias, Delphine.
Cette étude vise à approfondir la connaissance de la biologie de la reproduction de l'huître plate Ostrea edulis afin de mieux comprendre comment les caractéristiques de la biologie de reproduction de l'espèce peuvent rendre compte des phénomènes de déficits en hétérozygote et de relation positive entre hétérozygotie et croissance. En particulier, elle vise à analyser le succès reproducteur en milieu naturel et dans les populations maintenues en écloserie. Cette étude repose sur l'utilisation de marqueurs microsatellites qui, du fait de leur fort polymorphisme, représentent des outils puissants d'assignation de parenté. Une première partie de l'étude a consisté à analyser les pontes issues de femelles incubantes prélevées dans le milieu naturel. Le nombre...
Tipo: Text
Palavras-chave: Ostrea edulis; Microsatellites; Biologie de la reproduction; Variance du succès reproducteur.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00370/48167/48281.pdf

Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment
Provedor de dados: 5
Autores: Morvezen, Romain; Cornette, Florence; Charrier, Gregory; Guinand, Bruno; Lapegue, Sylvie; Boudry, Pierre; Laroche, Jean.
We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops.
Tipo: Text
Palavras-chave: Microsatellites; Polymerase chain reaction; Parentage; Assignment; Bivalve mollusc.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00137/24822/22907.pdf

Diversité et différentiation génétique chez l'huître plate européenne : de la population à l'individu (Actes)
Provedor de dados: 5
Autores: Boudry, Pierre; Launey, Sophie; Diaz-almela, A.; Naciri-graven, Yamama; Ledu, Christophe; Mira, S.; Taris, Nicolas; Bonhomme, Francois; Lapegue, Sylvie.
PCR-based DNA markers enable the study of diversity and differentiation of marine organisms at different stages of their life cycle. Our study of European fiat oyster (Ostrea edulis) populations aimed to describe their spatial and temporal dynamics. First, we analyzed adults sampled over the natural range of the species. An Atlantic/Mediterranean differentiation pattern was observed. Similar patterns of variation were found using allozymes, microsatellites and mitochondrial DNA, aIl which support an isolation by distance model. High variability was observed in the range of diversity between populations using a mitochondrial marker, a reflection of small effective population sizes in sorne locations. A 10fold quantitative difference was observed in Fst,...
Tipo: Text
Palavras-chave: Recruitment; Diversity; Molecular markers; Population genetics; Ostrea edulis; Flat oyster; Dynamique de recrutement; Diversité génétique; Microsatellites; Marqueurs moléculaires; Génétique des populations; Ostrea edulis; Huître plate.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-2851.pdf

Characterization of 27 microsatellite loci in the European flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Stockdale, Ruth; Boudry, Pierre; Beaumont, Andy R.; Lapegue, Sylvie.
The flat oyster Ostrea edulis is native to Europe and populations have been severely depleted by the parasite Bonamia ostreae since the 1980s. Additional genetic markers are required to improve population genetics study and linkage map development for selection for B. ostrea-resistance in this species. Here, we characterized 27 novel microsatellite loci for O. edulis. Number of alleles per locus ranged from 6 to 25 and observed heterozygosity between 0.375 and 1. Null alleles were suggested at a few loci but most loci were in Hardy-Weinberg agreement enabling their reliable use in further population and mapping genetics approaches.
Tipo: Text
Palavras-chave: Universal tailed primer labelling; Oysters; Ostrea edulis; Microsatellites.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6421.pdf

Natural hybridization between genetically differentiated populations of Crassostrea gigas and C-angulata highlighted by sequence variation in flanking regions of a microsatellite locus
Provedor de dados: 5
Autores: Huvet, Arnaud; Fabioux, Caroline; Mccombie, Helen; Lapegue, Sylvie; Boudry, Pierre.
The marine environment is of special interest for studying hybridization between closely related taxa because of the high dispersal potential of planktonic larvae, such as those of most bivalve species. The oysters Crassostrea angulata and C. gigas are known to be very close genetically and entirely inter-fertile under controlled conditions. However, hybridization in the wild had not been investigated, mainly due to the lack of nuclear diagnostic markers. In the present paper, we first estimated genetic differentiation between these 2 closely related taxa using 8 microsatellite markers. Interestingly, 5 markers displayed significant differences of allele size between taxa. The subsequent sequencing of alleles of one of these microsatellites showed several...
Tipo: Text
Palavras-chave: Allele size; Microsatellites; Genetic differentiation; Hybridization; Crassostrea gigas; Crassostrea angulata.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-3355.pdf

Microsatellite-associated heterosis in hatchery-propagated stocks of the shrimp Penaeus stylirostris
Provedor de dados: 5
Autores: Bierne, Nicolas; Bezuart, Ivan; Vonau, Vincent; Bonhomme, François; Bedier, Edouard.
Correlation between DNA microsatellite heterozygosity and growth rate was investigated in two hatchery-propagated stocks of the shrimp Penaeus stylirostris, which had been genetically isolated from wild founders for 17 generations, and from each other for five more generations. presumed demographic history of these populations suggested that they were maintained at small effective population sizes. and this was confirmed by significant changes in allelic frequencies between the two stocks. Despite a small sample size (n = 60) and a small number of loci screened, a significant positive correlation was detected between microsatellite tri-locus heterozygosity and growth rate in one of the two stocks (r = 0.3, P = 0.02). In the other stock (n = 48), the...
Tipo: Text
Palavras-chave: Penaeus stylirostris; Heterozygosity; Marker associated heterosis; Inbreeding depression; Microsatellites.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-753.pdf

Diversité génétique et dynamique du recrutement chez l'huître plate Ostrea edulis L. 2002
Provedor de dados: 5
Autores: Taris, Nicolas.
Cette étude aborde la diversité génétique d'une espèce de bivalve marin, Ostrea edulis et plus particulièrement les interactions existant entre génétique (flux de gènes) et démographie (dynamique de reproduction) à l'échelle de la population. Elle repose sur l'utilisation de marqueurs microsatellites pour tenter de comprendre les facteurs participant à la mise ne place d'une structuration génétique au sein de laquelle peuvent être observés des phénomènes de déficit en hétérozygotes et de ration positive entre hétérozygotie et valeur sélective. L'un des objectifs est en particulier de tester l'hypothèse de consanguinité à l'échelle locale. Pour cela, nous avons étudier la structure génétique de cohortes d'huîtres plates au cours d'une saison de reproduction...
Tipo: Text
Palavras-chave: Ostrea edulis; Génétique des Populations; Microsatellites; Diversité génétique; Dynamique de recrutement.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00033/14419/11714.pdf

Genealogical and molecular analysis of an Argentinean Angus seedstock herd
Provedor de dados: 46
Autores: Corva,P.M; Colavita,M.I; Legaz,G; Martínez,M.
Introduced in Argentina from the British Isles in 1879, the Angus progressively became the most popular beef cattle breed in the country. In this article the analysis of genealogical and molecular information generated in the registration process of progenies from an Angus seedstock herd is presented. The objectives were to analyze the genetic structure of the herd, and to use the acquired information to get insight of the history of the Angus breed in Argentina. Pedigree information from 16,611 individuals born between 1954 and 2012 was analyzed, including Black and Red Angus. Also available were genotypes of 17 microsatellites from 470 individuals born between 2005 and 2012. Identification of the most influential ancestors showed a trend that is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Population structure; Coancestry; Microsatellites; Inbreeding.
Ano: 2015
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332015000300002

A conservation genetic study of Rafflesia speciosa (Rafflesiaceae): patterns of genetic diversity and differentiation within and between islands
Provedor de dados: 16
Autores: Pelser, P.B.; Nickrent, D.L.; Barcelona, J.F.
Rafflesia speciosa is a threatened endo-holoparasitic species. It has several populations in the Central Panay Mountain Range (CPMR) of Panay island and a single population on Negros Island. Because R. speciosa is the only Philippine species of the genus that is not endemic to an individual island, it is a suitable species for improving our understanding of the factors underlying the high island endemism of Philippine Rafflesia. For this purpose and to inform the conservation management of R. speciosa, patterns of genetic diversity and differentiation were studied using 15 microsatellite loci and samples from nine populations. None of these populations shows evidence of inbreeding and R. speciosa has similar levels of heterozygosity as generally observed...
Tipo: Article / Letter to the editor
Palavras-chave: Microsatellites; Conservation; Negros; Panay; Philippines; Population genetics.
Ano: 2018
URL: http://www.repository.naturalis.nl/record/655789

Phytophthora betacei, a new species within Phytophthora clade 1c causing late blight on Solanum betaceum in Colombia
Provedor de dados: 16
Autores: Mideros, M.F.; Turissini, D.A.; Guayazán, N.; Ibarra-Avila, H.; Danies, G.; Cárdenas, M.; Myers, K.; Tabima, J.; Goss, E.M.; Bernal,, A.; Lagos, L.E.; Grajales, A.; Gonzalez, L.N.; Cooke, D.E.L.; Fry, W.E.; Grünwald, N.; Matute, D.R.; Restrepo, S.
Over the past few years, symptoms akin to late blight disease have been reported on a variety of crop plants in South America. Despite the economic importance of these crops, the causal agents of the diseases belonging to the genus Phytophthora have not been completely characterized. In this study, a new Phytophthora species was described in Colombia from tree tomato (Solanum betaceum), a semi-domesticated fruit grown in northern South America. Comprehensive phylogenetic, morphological, population genetic analyses, and infection assays to characterize this new species, were conducted. All data support the description of the new species, Phytophthora betacei sp. nov. Phylogenetic analyses suggest that this new species belongs to clade 1c of the genus...
Tipo: Article / Letter to the editor
Palavras-chave: Host specificity; Microsatellites; Oomycetes; Species delimitation; Tree tomato.
Ano: 2018
URL: http://www.repository.naturalis.nl/record/643330

Morphological and molecular data to describe a hybrid population of the Common toad (Bufo bufo) and the Spined toad (Bufo spinosus) in western France
Provedor de dados: 16
Autores: Trujillo, T.; Gutiérrez-Rodríguez, J.; Arntzen, J.W.; Martínez-Solano, I.
The use of hyper-variable markers across species is often hindered by low cross-species amplification success, a reduced level of polymorphism or a high frequency of null alleles. However, optimizing sets of reliable and informative markers that can be consistently amplified and scored across taxa is key to address questions about patterns of genetic diversity and structure, hybridization and speciation. Here we present 14 newly developed microsatellite markers in the Spined toad (Bufo spinosus), assess their polymorphism in two Iberian populations and test for cross-species amplification in the closely related Common toad (Bufo bufo). We then use the 12 loci co-amplifying in both species to the study of a morphologically intermediate population (Moyaux)...
Tipo: Article / Letter to the editor
Palavras-chave: Amphibians; Cross-amplification; Genetic polymorphism; Hybridization; Microsatellites; Morphology.
Ano: 2017
URL: http://www.repository.naturalis.nl/record/623750

The endangered Dama dama mesopotamica Brooke, 1875: genetic variability, allelic loss and hybridization signals
Provedor de dados: 16
Autores: Fernández-García, J.L.
The Persian fallow deer (Dama dama mesopotamica) formerly widespread in the Middle East was described scientifically at the end of the 19th century and considered extinct ever since. In 1956 it was rediscovered in south-western Iran. As a result, several countries have undertaken actions to reintroduce this subspecies in its native territory. In 2007 the Christian Oswald Foundation, in close cooperation with Iranian institutions, launched plans of in situ and ex situ breeding actions, with its centre in the German Von Opel Zoo and with cooperative Mediterranean partner countries as Israel, to support conservation efforts under scientific control. We performed genetic studies to study the suspected hybridization with European fallow deer (Dama dama dama)...
Tipo: Article / Letter to the editor
Palavras-chave: Breeding and restocking plans; European fallow deer; Fallow deer distinctiveness; Allele loss; Microsatellites; Persian fallow deer; 42.84.
Ano: 2012
URL: http://www.repository.naturalis.nl/record/434754

Evaluating methodologies for species delimitation: the mismatch between phenotypes and genotypes in lichenized fungi (Bryoria sect. Implexae, Parmeliaceae)
Provedor de dados: 16
Autores: Boluda, C.G.; Rico, V.J.; Divakar, P.K.; Nadyeina, O.; Myllys, L.; McMullin, R.T.; Zamora, J.C.; Scheidegger, C.; Hawksworth, D.L.
In many lichen-forming fungi, molecular phylogenetic analyses lead to the discovery of cryptic species within traditional morphospecies. However, in some cases, molecular sequence data also questions the separation of phenotypically characterised species. Here we apply an integrative taxonomy approach ‒ including morphological, chemical, molecular, and distributional characters ‒ to re-assess species boundaries in a traditionally speciose group of hair lichens, Bryoria sect. Implexae. We sampled multilocus sequence and microsatellite data from 142 specimens from a broad intercontinental distribution. Molecular data included DNA sequences of the standard fungal markers ITS, IGS, GAPDH, two newly tested loci (FRBi15 and FRBi16), and SSR frequencies from 18...
Tipo: Article / Letter to the editor
Palavras-chave: Chemotypes; Cryptic species; Haplotypes; Incomplete lineage sorting; Integrative taxonomy; Microsatellites; Speciation; Species concepts.
Ano: 2019
URL: http://www.repository.naturalis.nl/record/660277

Evidence of polygamy in the socially monogamous Amazonian fish Arapaima gigas (Schinz, 1822) (Osteoglossiformes, Arapaimidae)
Provedor de dados: 83
Autores: Farias,Izeni Pires; Leão,Adam; Almeida,Yane Santos; Verba,Júlia Tovar; Crossa M.,Marcelo; Honczaryk,Alexandre; Hrbek,Tomas.
Arapaima gigas is one of the largest freshwater fishes of the world. It is socially monogamous, forming pairs, constructing a nest and providing parental care. We performed a paternity analysis under three scenarios in captive, semi-natural and natural areas using 10 microsatellite markers. As a positive control, we analyzed three pairs and their offspring isolated individually in artificial breeding ponds (a priori very high probability of monogamy). We then analyzed two samples of offspring from large artificial ponds with multiple adults but only one reproductive pair (a priori high probability of monogamy), two samples from semi-natural breeding station with multiple adults but only one reproductive pair (a priori high probability of monogamy), and a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mate choice; Microsatellites; Multiple paternity; Pirarucu; Polygamy.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000100195

Population genetics of three threatened catfish species in heterogeneous environments of the Cauca River, Colombia
Provedor de dados: 83
Autores: Restrepo-Escobar,Natalia; Yepes-Acevedo,Anny Johanna; Márquez,Edna Judith.
ABSTRACT Neotropical catfishes Ageneiosus pardalis, Pimelodus grosskopfii and Sorubim cuspicaudus are migratory fishes of commercial importance that exhibit decreasing populations due to overfishing and other anthropic interventions. This study used species-specific microsatellite loci to test the hypothesis that threatened fish populations show genetic vulnerability signs and are genetically structured in the middle and lower sections of the Cauca River. The studied species exhibit genetic diversity levels higher than the average values reported for Neotropical Siluriformes; however, they seem to have suffered recent bottlenecks and they present significant endogamy levels that are higher for the critically endangered catfish P. grosskopfii. Furthermore,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Freshwater fish; Genetic diversity; Genetic structure; Microsatellites; Siluriformes.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252021000100204

Genetic evidence supports polygamous mating system in a wild population of Prochilodus lineatus (Characiformes: Prochilodontidae), a Neotropical shoal spawner fish
Provedor de dados: 83
Autores: Ribolli,Josiane; Miño,Carolina Isabel; Scaranto,Bianca Maria Soares; Reynalte-Tataje,David Augusto; Zaniboni Filho,Evoy.
ABSTRACT Behavioral observations made on fish have revealed remarkably diverse reproductive strategies, including polygamy by both sexes. Still, to date, most Neotropical species remain unstudied as to whether the observed reproductive behavior in natural populations correlates with their genetic mating systems. Here, we investigated the genetic mating system of a wild population of Prochilodus lineatus settled in the Middle Uruguay River basin. By using sibship reconstruction and parental inference methods based on microsatellites’ genotypes, we inferred 45 females and 47 males as potential parents of the 87 larvae analyzed. We found evidence supporting polygamous mating in both sexes: while a high percentage of males (44.7%) fertilized the eggs of one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic mating system; Microsatellites; Migratory fish; Parentage; Relatedness.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000200215

Genetic diversity of Brazilian Pantaneiro horse and relationships among horse breeds
Provedor de dados: 86
Autores: Sereno,Fabiana Tavares Pires de Souza; Sereno,José Robson Bezerra; Vega-Pla,Jose Luis; Kelly,Lucy; Bermejo,Juan Vicente Delgado.
The objective of this work was to evaluate the genetic diversity of Brazilian Pantaneiro horse by microsatellite markers, investigate the effect of genetic bottlenecks and estimate genetic differentiation among four horse breeds. Genetic variation was estimated through allele frequencies and mean breed heterozygosity. Nei's genetic distances among the breeds Pantaneiro, Thoroughbred, Arabian, Spanish Pure Breed (Andalusian), and Uruguay Creole were calculated, and it was used to construct an UPGMA dendrogram. Clustering at different K values was calculated to infer population structure and assign individuals to populations. Nei's distances showed a minimum distance between Pantaneiro horse and Spanish Pure Breed (0.228), and similar distances from Spanish...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Equus caballus; Animal conservation; Molecular markers; Microsatellites; Genetic resources conservation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000500007

Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analyses
Provedor de dados: 86
Autores: Koehler-Santos,Patrícia; Dornelles,Ana Lúcia Cunha; Freitas,Loreta Brandão de.
The objective of this work was to characterize mandarin (Citrus spp.) germplasm from Southern Brazil by morphological and molecular analyses. Thirty seven cultivars from 34 distinct mandarin varieties were evaluated by morphological and agronomic traits of leaves, flowers and fruits, and by microsatellite markers. The morphological and agronomic characteristics suggested that almost all varieties can be produced for commercial use, and some, as the Satsuma variety, are recommended for breeding programs. Pooled DNA samples from 1-5 plants belonging to each cultivar were tested. Eight of the nine primers detected polymorphisms. Specific markers were found for some accessions. The dendrogram constructed with the morphological results divided the 37 cultivars...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus; Genetic variation; Microsatellites; Breeding methods.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2003000700003

Genetic variability of Brazilian rice landraces determined by SSR markers
Provedor de dados: 86
Autores: Borba,Tereza Cristina de Oliveira; Mendes,Clistiane dos Anjos; Guimarães,Élcio Perpétuo; Brunes,Tuliana Oliveira; Fonseca,Jaime Roberto; Brondani,Rosana Vianello; Brondani,Claudio.
The objective of this study was to evaluate the genetic variability of rice (Oryza sativa) landraces collected in Brazilian small farms. Twelve simple sequence repeat (SSR) markers characterized 417 landraces collected in 1986, 1987 and 2003, in the state of Goiás, Brazil. The number of landraces with long and thin grain type increased in the evaluated period, probably due to market demand. Based on the molecular data, the genetic variability increased during this period and, as per to the factorial correspondence analysis, most of the accessions were grouped according to the year of collection. The incorporation of modern rice cultivars in landrace cultivation areas and the selection carried out by small farmers are the most probable factors responsible...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oryza sativa; Genotyping; Microsatellites; Participatory breeding; Rice breeding.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000700009

Genetic diversity between improved banana diploids using canonical variables and the Ward-MLM method
Provedor de dados: 86
Autores: Pereira,Valquíria Martins; Borges,Cristine Vanz; Brandão,Lívia Pinto; Oliveira,Larissa Santos; Souza,Cintia Paula Feitosa; Gonçalves,Zalmar Santana; Silva,Sebastião de Oliveira e; Santos-Serejo,Janay Almeida dos; Ferreira,Claudia Fortes; Amorim,Edson Perito; Ledo,Carlos Alberto da Silva.
The objective of this work was to estimate the genetic diversity of improved banana diploids using data from quantitative analysis and from simple sequence repeats (SSR) marker, simultaneously. The experiment was carried out with 33 diploids, in an augmented block design with 30 regular treatments and three common ones. Eighteen agronomic characteristics and 20 SSR primers were used. The agronomic characteristics and the SSR were analyzed simultaneously by the Ward-MLM, cluster, and IML procedures. The Ward clustering method considered the combined matrix obtained by the Gower algorithm. The Ward-MLM procedure identified three ideal groups (G1, G2, and G3) based on pseudo-F and pseudo-t² statistics. The dendrogram showed relative similarity between the G1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Musa; Hybrids; Microsatellites; Morphological characteristics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012001000010

Genetic diversity and structure of native maize races from Northwestern Mexico
Provedor de dados: 86
Autores: Vega-Alvarez,Isrrael; Santacruz-Varela,Amalio; Rocandio-Rodríguez,Mario; Córdova-Téllez,Leobigildo; López-Sánchez,Higinio; Muñoz-Orozco,Abel; Hernández-Bautista,Aurelio.
Abstract: The objective of this work was to evaluate the genetic diversity of nine maize races (Zea mays ssp. mays) from Northwestern Mexico and one population of teosinte of the Balsas race (Zea mays ssp. parviglumis). A total of 649 alleles were identified, with an average of 20.9 alleles per locus using 31 microsatellite loci; 84.3% of them were polymorphic loci with a 0.49 expected heterozygosity. Graphic representation of principal coordinate analysis (PCoA) showed broad variation and population distribution. The highest probabilistic value obtained with the ΔK criterion confirmed the existence of five population groups clustered by the Bayesian model. This grouping coincided with the population distribution observed in the PCoA graph. Maize races...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays; Conservation strategies; Landraces; Microsatellites; Plant breeding.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001101023

Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance
Provedor de dados: 86
Autores: Pinto,Fernanda de Oliveira; Maluf,Mirian Perez; Guerreiro-Filho,Oliveiro.
The objective of this work was to identify expressed simple sequence repeats (SSR) markers associated to leaf miner resistance in coffee progenies. Identification of SSR markers was accomplished by directed searches on the Brazilian Coffee Expressed Sequence Tags (EST) database. Sequence analysis of 32 selected SSR loci showed that 65% repeats are of tetra-, 21% of tri- and 14% of dinucleotides. Also, expressed SSR are localized frequently in the 5'-UTR of gene transcript. Moreover, most of the genes containing SSR are associated with defense mechanisms. Polymorphisms were analyzed in progenies segregating for resistance to the leaf miner and corresponding to advanced generations of a Coffea arabica x Coffea racemosa hybrid. Frequency of SSR alleles was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leucoptera coffeella; Microsatellites; Molecular markers.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000300011

Morphological and molecular diversity among cassava genotypes
Provedor de dados: 86
Autores: Mezette,Thiago Fonseca; Blumer,Caroline Groppo; Veasey,Elizabeth Ann.
The objective of this work was to characterize morphologically and molecularly the genetic diversity of cassava accessions, collected from different regions in Brazil. A descriptive analysis was made for 12 morphological traits in 419 accessions. Data was transformed into binary data for cluster analysis and analysis of molecular variance. A higher proportion of white or cream (71%) root cortex color was found, while flesh colors were predominantly white (49%) and cream (42%). Four accession groups were classified by the cluster analysis, but they were not grouped according to their origin, which indicates that diversity is not structured in space. The variation was greater within regions (95.6%). Sixty genotypes were also evaluated using 14 polymorphic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Genetic diversity; Germplasm; Microsatellites; Morphological traits.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000500007

Genetic associations in the detection of QTLs for wheat spike-related traits
Provedor de dados: 86
Autores: Brbaklić,Ljiljana; Trkulja,Dragana; Kondić-Špika,Ankica; Hristov,Nikola; Denčić,Srbislav; Mikić,Sanja; Tomičić,Marina; Kobiljski,Borislav.
The objective of this work was to assess the genetic diversity and population structure of wheat genotypes, to detect significant and stable genetic associations, as well as to evaluate the efficiency of statistical models to identify chromosome regions responsible for the expression of spike-related traits. Eight important spike characteristics were measured during five growing seasons in Serbia. A set of 30 microsatellite markers positioned near important agronomic loci was used to evaluate genetic diversity, resulting in a total of 349 alleles. The marker-trait associations were analyzed using the general linear and mixed linear models. The results obtained for number of allelic variants per locus (11.5), average polymorphic information content value...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triticum aestivum; Genetic resources; Microsatellites; Population structure; Spike-related traits.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2015000200149

Development of herbicide-tolerant irrigated rice cultivars
Provedor de dados: 86
Autores: Rangel,Paulo Hideo Nakano; Moura Neto,Francisco Pereira; Fagundes,Paulo Ricardo Reis; Magalhães Junior,Ariano Martins de; Morais,Orlando Peixoto de; Schmidt,Andréa Branco; Mendonça,João Antônio; Santiago,Carlos Martins; Rangel,Priscila Nascimento; Cutrim,Veridiano dos Anjos; Ferreira,Márcio Elias.
The objective of this work was to develop new irrigated rice lines tolerant to imidazolinone herbicides. The backcross breeding procedure was used to transfer the imidazolinone tolerance allele from mutant 93AS3510 to the recurrent parents 'BRS 7 Taim' and 'BRS Pelota'. Individual herbicide-tolerant plants were selected in each generation, for three backcrossings (RC1 to RC3), followed by three selfing generations (RC3F1 to RC3F3). The best four RC3F3 lines for agronomic traits were genotyped with 44 microsatellite markers. The observed conversion index of the new imidazolinone-tolerant lines varied from 91.86 to 97.67%. Pairwise genetic distance analysis between these lines and 22 accessions from the Embrapa's Rice Germplasm Bank clustered the new lines...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oryza sativa; Backcrossing; Clearfield rice; Microsatellites; Red rice.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000700010

Microsatellite-dense genetic map: towards genome coverage in a tropical maize (Zea mays L.) population
Provedor de dados: 88
Autores: Lima,Milena De Luna Alves; Souza Jr.,Cláudio Lopes de; Souza,Anete Pareira de.
Dense molecular genetic maps are used for an efficient quantitative trait loci (QTL) mapping and in the marker-assisted selection programs. A dense genetic map was generated with 139 microsatellite markers using 256 F2 plants generated by the crossing of two tropical maize inbred lines (L-02-03D and L-20-01F). This map presented 1,858.61 cM in length, where 10 linkage groups were found spanned, with an average interval of 13.47 cM between adjacent markers. Seventy seven percent of the maize genetic mapping bins were covered, which means an increase of 14% coverage in relation to the previous tropical maize maps. The results provide a more detailed and informative genetic map in a tropical maize population representing the first step to make possible the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dense genetic map; Genome coverage; Microsatellites; Tropical maize; Zea mays L.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042009000300009

GENETIC DIVERSITY AND STRUCTURE OF Oenocarpus mapora GERMPLASM CONSERVED AT EASTERN AMAZON
Provedor de dados: 94
Autores: MOURA,ELISA FERREIRA; OLIVEIRA,MARIA DO SOCORRO PADILHA DE; SILVA,DIEHGO TULOZA DA; PONTES,LÍGIA CRISTINE GONÇALVES.
ABSTRACT  The aim of this study was to evaluate the genetic diversity and structure in the germoplasm of Oenocarpus mapora conserved at Eastern Amazon. Thus, 88 individuals were genotyped with five microsatellite loci. These individuals belong to 24 accessions that were sampled in eight sample places of three Brazilian Amazon states conserved at the Active Germplasm Bank (AGB) of Embrapa Eastern Amazon. All loci were polymorphic and they generated 85 alleles with an average of 17 alleles per loci. Total genetic diversity (HE) was 0.48. Sample places were considered genetically distinct, with ?p = 0.354. The analysis of molecular variance (AMOVA) identified that the genetic portion among areas was of 36.14% and within 63.86%. The Nei distances varied from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arecaceae; Molecular markers; Microsatellites; Plant genetic resources.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452015000400984

Genetic and chemical characterization of avocado commercial cultivars avocado of Risaralda Colombia
Provedor de dados: 94
Autores: Álvarez,Gloria Edith Guerrero; Gutiérrez,Ana María López; Valencia,Katalina Ángel; Mossos,Paula Sandoval; Rozo,Diana Lucía Suárez; Hurtado,Nathalia Cardona.
Abstract  This research aimed at performing the molecular characterization of commercial Papelillo avocado (Persea americana cv Lorena) cultivars from the municipality of Marsella (Risaralda, Colombia), as well as the physicochemical analysis and antioxidant activity assessment of the pulp and seed. An evaluation of 50 individuals among commercial varieties and possible patterns was performed using 17 microsatellite markers. Proximate analysis of the pulp was performed, and the fatty acid profile of oils, the antioxidant activity by the DPPH and FRAP methods, and the total phenolic content were evaluated. From the cluster analysis, Dice index, and Principal Coordinates Analysis, it became evident that all the individuals showed a tendency to group by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; DNA; Fatty acids; Microsatellites; Persea americana Mill.; Total phenols.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452020000500902

Managing genetic diversity and society needs
Provedor de dados: 96
Autores: Mariante,Arthur da Silva; Egito,Andréa Alves; Albuquerque,Maria do Socorro Maués; Paiva,Samuel Rezende; Ramos,Alexandre Floriani.
Most livestock are not indigenous to Brazil. Several animal species were considered domesticated in the pre-colonial period, since the indigenous people manage them as would be typical of European livestock production. For over 500 years there have been periodic introductions resulting in the wide range of genetic diversity that for centuries supported domestic animal production in the country. Even though these naturalized breeds have acquired adaptive traits after centuries of natural selection, they have been gradually replaced by exotic breeds, to such an extent, that today they are in danger of extinction To avoid further loss of this important genetic material, in 1983 Embrapa Genetic Resources and Biotechnology decided to include conservation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal genetic resources; Brazilian conservation program; Genetic variability; Microsatellites.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982008001300016

Genetic diversity of the Brazilian Creole cattle Pé-duro assessed by microsatellites and mitochondrial DNA
Provedor de dados: 96
Autores: Oliveira,Ana Paula Ferreira de; Carvalho,José Herculano de; Miretti,Marcos Mateo; Lara,Maria Aparecida Cassiano; Contel,Eucleia Primo Betioli.
The objective of this study was to describe the genetic diversity and structure of the largest Pé-duro population by assessing variation at ten autosomal microsatellite (STR) loci and mitochondrial DNA (mtDNA) sequences. The mean expected heterozygosity was 0.755, the mean observed heterozygosity was 0.600 and significant inbreeding coefficient (Fis) and deviations from the Hardy-Weinberg equilibrium in most of analyzed loci demonstrate the impact of inbreeding and homozygosis on this population. A more in-depth genetic analysis could be achieved by expanding the STR list. The analysis of mtDNA provided evidence of ancestral African taurine haplotypes in Pé-duro and excluded maternal Zebuine introgression. In this report, the main Pé-duro population is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bos taurus; Cattle; Conservation; Genetic diversity; Microsatellites; Mitochondrial DNA.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001100003

Influence of forest type and host plant genetic relatedness on the canopy arthropod community structure of Quercus crassifolia
Provedor de dados: 97
Autores: Tovar-Sánchez,Efraín; Martí-Flores,Erwin; Valencia-Cuevas,Leticia; Mussali-Galante,Patricia.
BACKGROUND: Quercus crassifolia is an oak species with characteristics of foundation species, which is a canopy dominant element of different forest types that supports a wide diversity of associated species. Therefore, it is an excellent system to address important ecological questions. We analyzed the effect of individual genetic relatedness of the host plant, forest type (Abies-Quercus, Quercus-Pinus, and Quercus forest), and season (dry vs. rainy) on the canopy arthropod community structure. Thirty oak canopies were fogged (five individuals/season/forest type). RESULTS: We identified 442 arthropod species belonging to 22 orders. The highest values of density, diversity, and richness were recorded during the rainy season for each forest type. Also, the...
Tipo: Journal article
Palavras-chave: Quercus; Foundation species; Individual genetic diversity; Microsatellites; Forest type; Arthropods community.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2015000100007

Comparative genetic structure in pines: evolutionary and conservation consequences
Provedor de dados: 97
Autores: DELGADO,PATRICIA; CUENCA,ARGELIA; ESCALANTE,ANA E.; MOLINA-FREANER,FRANCISCO; PIÑERO,DANIEL.
Pines have been the focus of several studies that estimate population genetic parameters using both allozymes and chloroplast single sequence repeats (SSRs). Also, the genus has also been recently studied using molecular systematics so that we now have a more clear understanding of their evolutionary history. With this background we studied comparatively the genetic structure in pines. Expected heterozygosity is particularly constant with a 99 % confidence interval between 0.19 and 0.23 in species that have been studied until now using allozymes. There is a significant proportion of species (9/41) that show high population differentiation estimates (Fst = or larger than 0.15) and five of these have large and wingless seeds probably associated with low...
Tipo: Journal article
Palavras-chave: Pinus; Genetic structure; Outcrossing rates; Microsatellites; Allozymes.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2002000100004

Genetic variability in Pseudoplatystoma reticulatum from a breeding program in Brazil
Provedor de dados: 124
Autores: Albuquerque,Daniele Menezes; Oliveira,Carlos Antonio Lopes de; Rodriguez,Maria Del Pilar Rodriguez; Ribeiro,Ricardo Pereira.
ABSTRACT The aim of this study was to characterise genetic variability in five groups of Pseudoplatystoma reticulatum: Mato Grosso I (MT-I), Mato Grosso II (MT-II), Mato Grosso III (MT-III), Mato Grosso do Sul I (MS-I) and Mato Grosso do Sul II (MS-II), taken from a centre for genetic improvement. In order to determine the allelic frequency, observed heterozygosity (HO), inbreeding coefficients (FIS), Hardy-Weinberg equilibrium (HW-p) and genetic differentiation, eight polymorphic microsatellite loci (Pcor01, Pcor05, Pcor08, Pcor10, Ppu01, Ppu04, Ppu09 and Ppu10) were selected, in which a total of 62 alleles were observed in the individuals (n = 211). The MS-I population had the highest (Pcor08 allele 83: 0.5000) and lowest (Pcor10 allele 50: 0.1667) mean...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cachara; Inbreeding coefficient; Allelic Frequency; Population genetics; Microsatellites.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000100402

Identification and validation of novel EST-SSR markers in olives
Provedor de dados: 63
Autores: Arbeiter,Alenka Baruca; Hladnik,Matjaž; Jakše,Jernej; Bandelj,Dunja.
ABSTRACT: The olive (Olea europaea L.) is a leading oil crop in the Mediterranean area. Limited information on the inheritance of agronomic significant traits hinders progress in olive breeding programs, which encourages the development of markers linked to the traits. In this study, we report on the development of 46 olive simple sequence repeat (SSR) markers, obtained from 577,025 expressed sequence tags (ESTs) in developing olive fruits generated in the framework of the Slovenian national olive transcriptome project. Sequences were de novo assembled into 98,924 unigenes, which were then used as a source for microsatellites searching. We identified 923 unigenes that contained 984 SSRs among which dinucleotide SSRs (36 %) were the most abundant, followed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Expressed sequence tags; Microsatellites; Genic markers; Genetic diversity; Functional annotation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000300215

Development of an SSR-based identification key for Tunisian local almonds
Provedor de dados: 63
Autores: Gouta,Hassouna; Ksia,Elhem; Buhner-Zaharieva,Tatiana; Mliki,Ahmed; Gogorcena,Yolanda.
Ten simple sequence repeat (SSR) loci were used to study polymorphism in 54 almond genotypes. All genotypes used in this study originated from almond-growing areas in Tunisia with different climatic conditions ranging from the sub-humid to the arid and are preserved in the national collection at Sidi Bouzid. Using ten SSR, 130 alleles and 250 genotypes were revealed. In order to develop an identification key for each accession, the data were analysed separately for each microsatellite marker. The most polymorphic microsatellite (CPDCT042) was used as a first marker. Two microsatellite loci (CPDCT042 and CPDCT025) were sufficient to discriminate among all accessions studied. Neighbour-joining clustering and principal coordinate analysis were performed to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prunus dulcis Mill; Genetic diversity; Genetic resources; Microsatellites; Molecular characterization.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000200004

Genetic linkage map of chicken chromosome 1 from a Brazilian resource population
Provedor de dados: 63
Autores: Nones,Kátia; Ledur,Mônica Corrêa; Ruy,Deborah Clea; Baron,Erica Elias; Moura,Ana Silvia Alves Meira Tavares; Coutinho,Luiz Lehmann.
A genetic map provides insight into genome organization and chromosomal location of markers and genes, and is important for quantitative trait loci (QTL) mapping. The objective of this study was to use a Brazilian resource population to construct a linkage map for chicken chromosome 1. Eighty microsatellite markers were tested and 26 informative markers were typed in an experimental F2 population developed by two generations of crossbreeding between a broiler sire line and a layer line. A total of 649 F2 individuals from seven full-sib families with about 95 F2 offspring each were genotyped. Multi-locus linkage analysis resulted in a chromosome 1 map of 26 ordered markers. Locus order was in general agreement with other published linkage maps, except for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Poultry; Mapping.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000100003

การหาเครื่องหมายพันธุกรรมที่เกี่ยวข้องกับความสมบูรณ์พันธุ์ในโคนมภายใต้สภาพร้อนชื้น: รายงานการวิจัยฉบับสมบูรณ์
Provedor de dados: 106
Autores: Monchai Duangjinda; Suporn Katawatin; Yupin Phasuk; Tawin Wongpalub.
Tipo: PhysicalObject
Palavras-chave: Dairy cattle; Hot stress; Humid condition; Genetic marker; Animal breeding; Dna; Microsatellites; Allele; Heat tolerance; Fertility; โคนม; ภูมิอากาศ; ความร้อน; ความชื้น; ความเครียด; การทนความร้อน; การปรับปรุงพันธุ์สัตว์; เครื่องหมายพันธุกรรม; ดีเอ็นเอ; ความทนทานต่อความเครียด; ไมโครแซทเทิลไลท์; อัลลีล; ความสมบูรณ์พันธุ์; การผลิตน้ำนม.
Ano: 2009
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5717

Molecular characterization of Iranian dromedaries using microsatellite markers
Provedor de dados: 109
Autores: Mohammadabadi.,Mohammadreza; Meymandi,Mehrdad Ghasemi; Montazen,Mahdieh; Afanasenko,Volodymyr; Kalashnyk,Oleksandr.
Abstract Considering the importance of maintaining the genetic diversity in native animals, this study conducted to analyse genetic diversity in dromedary populations in the north of Kerman province, Iran, using eight autosomal microsatellite markers. Eighty-one blood samples were collected from five different populations and DNA was extracted. The highest and the lowest alíele number and effective alíeles were shown in YWLL08 (21 and 4) and VOLP32 (14.97 and 3.11), respectively. The expected heterozygosity varied from 0.778 in Sahra-e Jahad population to 0.847 in Nogh population. The test for Hardy-Weinberg equilibrium showed significant deviations in most loci. The mean multilocus Fst value (0.057) suggested that differentiation is modérate between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Iranian dromedary populations; Microsatellites; Population genetic structure.
Ano: 2020
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122020000400321

Phenotypic and genotypic characterization and compatibility among genotypes to select elite clones of cupuassu
Provedor de dados: 36
Autores: ALVES,Rafael Moysés; SILVA,Carlos Rogério de Sousa; ALBUQUERQUE,Paulo Sérgio Bevilaqua de; SANTOS,Vinicius Silva dos.
ABSTRACT The expansion of the genetic base of cultivated materials is an ongoing activity of the cupuassu (Theobroma grandiflorum) breeding program. However, the parents involved need to be genotypically and phenotypically characterized to ensure compatibility of crossings, as well as to assist in the selection of more promising individuals for hybridization. This study aimed to identify and select T. grandiflorum clones that are compatible and genetically divergent using tools such as the estimates of genotypic, phenotypic, and combined distances, as well as the compatibility rates among clones. The genetic distance analysis of the clones was performed with 14 heterologous microsatellite primers of cocoa (Theobroma cacao) that amplify the DNA of cupuassu....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Tropical fruit; Diversity; Plant breeding; Theobroma grandiflorum.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672017000300175

DNA fingerprinting based on SSR amplification profiles for Piper species identification (Piperaceae)
Provedor de dados: 39
Autores: Christ,Jheniffer Abeldt; Hollunder,Renan Köpp; Carvalho,Marina Santos; Ferreira,Marcia Flores da Silva; Garbin,Mário Luís; Carrijo,Tatiana Tavares.
ABSTRACT DNA fingerprinting based on SSR amplification profiles was applied to native species of Piper from the Atlantic Forest to compare the utility of this type of molecular marker with the morphological characters traditionally applied in Piper taxonomy and identification. Fifty-one SSR markers developed for four species of Piper native to Asia and Mesoamerica were applied to 16 species, together with 63 morphological characters, for species characterization. Molecular and morphological data were analysed by cluster analysis, followed by a cluster sharpness test and the construction of a heat map to visualize the association of characters with species groups. A multivariate regression tree determined the number of loci needed for species...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heat map; Microsatellites; Morphology; Multivariate analyses; Taxonomy.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000400511

Genetic structure of Dicksonia sellowiana Hook. (Dicksoniaceae) reveals clinal distribution along the latitudinal gradient of the Atlantic Forest
Provedor de dados: 39
Autores: Fagundes,Bruna Saviatto; Poersch,Maria Augusta; Santos,Jaqueline dos; Gaglioti,André Luiz; Labiak,Paulo Henrique; Muschner,Valeria Cunha.
ABSTRACT Dicksonia sellowiana is the only species of the genus occurring in Brazil. Its distribution is restricted to humid areas of the Atlantic Forest biome. The distribution pattern of biodiversity in this biome is known to have been influenced by historical and environmental factors, although the pattern for ferns remains unknown. This study is first to describe the genetic structure of D. sellowiana along the latitudinal gradient of the Atlantic Forest biome. We use microsatellite markers to estimate genetic diversity and structure for 267 individuals representing 14 populations of D. sellowiana from the Atlantic Forest. The results (Ho, He, Fst, Fis, distance genetic) support the hypothesis of a pattern of biodiversity discontinuity. We found greater...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Neotropics; Gene flow; Genetic diversity; Microsatellites; Tree fern.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000400712

Linkage disequilibrium and population structure in Fragaria chiloensis revealed by SSR markers transferred from commercial strawberry
Provedor de dados: 1
Autores: Oñate, Felipe Alberto; Hasbún, Rodrigo; Mora, Freddy; Figueroa, Carlos Rodrigo.
 The Chilean strawberry [Fragaria chiloensis (L.) Mill.] is the maternal progenitor of the commercial strawberry (Fragaria ´ ananassa Duch.), which is characterized by fruits with high organoleptic quality and is well-suited to areas where drought and salinity represent a constraint on crop growth and productivity. We examined the patterns of linkage disequilibrium, genetic diversity and population structure among 54 accessions of F. chiloensis to understand the genetic basis of this species. We used a core microsatellite marker set (n = 95) from a consensus linkage map of strawberry. A transferability rate of 82.1% (78/95) was found, and 38 markers were selected for this study. The SSR primers produced a total of 259 alleles, which varied between 112 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Microsatellites; Chilean strawberry; Marker transferability.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/34966

Linkage disequilibrium and population structure in Fragaria chiloensis revealed by SSR markers transferred from commercial strawberry
Provedor de dados: 1
Autores: Oñate, Felipe Alberto; Hasbún, Rodrigo; Mora, Freddy; Figueroa, Carlos Rodrigo.
 The Chilean strawberry [Fragaria chiloensis (L.) Mill.] is the maternal progenitor of the commercial strawberry (Fragaria ´ ananassa Duch.), which is characterized by fruits with high organoleptic quality and is well-suited to areas where drought and salinity represent a constraint on crop growth and productivity. We examined the patterns of linkage disequilibrium, genetic diversity and population structure among 54 accessions of F. chiloensis to understand the genetic basis of this species. We used a core microsatellite marker set (n = 95) from a consensus linkage map of strawberry. A transferability rate of 82.1% (78/95) was found, and 38 markers were selected for this study. The SSR primers produced a total of 259 alleles, which varied between 112 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Microsatellites; Chilean strawberry; Marker transferability.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/34966

Diversidade genética em populações-núcleo de Eucalyptus grandis - doi: 10.4025/actasciagron.v32i4.3727
Provedor de dados: 1
Autores: Souza, Helenize Gabriela de; UNESP; Doria, Karolina Marie Alix Benedictte Van Sebroech; UNESP; Basseto, Marco Antonio; UNESP; Rosa, Daniel Dias; UNESP-FCA, Programa de Pós-graduação em Proteção de Plantas; Furtado, Edson Luiz; UNESP; Marino, Celso Luis; UNESP - IBB, Depto de Genética.
No melhoramento genético de espécies florestais, uma população base ou indivíduos superiores pré-selecionados tem importância fundamental para a manutenção do programa. Indivíduos de melhores procedências e de ampla base genética propiciam a obtenção de ganhos de forma contínua. O objetivo deste trabalho foi avaliar a diversidade genética em duas populações-núcleo de Eucalyptus grandis. Foram avaliados 39 indivíduos, sendo 19 pertencentes à população 1 e 20, à população 2, utilizando-se 14 primers microssatélite. Os fragmentos foram identificados e analisados a partir dos programas GeneScan e Genotyper, utilizando-se um sequenciador automático ABI Prism 3100. O número de alelos encontrados para cada primer variou de cinco a 15 para a população 1 e, de 8 a...
Palavras-chave: 5.01.03.05-9 populações-núcleo; Eucalyptus; Melhoramento; Microssatélites Melhoramento genético Florestal nucleus populations; Eucalyptus; Improvement; Microsatellites.
Ano: 2010
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3727

Genetic dissimilarity among sweet potato genotypes using morphological and molecular descriptors
Provedor de dados: 1
Autores: Andrade, Elisângela Knoblauch Viega de; Andrade júnior, Valter Carvalho de; Laia, Marcelo Luiz de; Fernandes, José Sebastião Cunha; Oliveira, Altino Junior Mendes; Azevedo, Alcinei Mistico.
 This study aimed to evaluate the genetic dissimilarity among sweet potato genotypes using morphological and molecular descriptors. The experiment was conducted in the Olericulture Sector at Federal University of Jequitinhonha and Mucuri Valleys (UFVJM) and evaluated 60 sweet potato genotypes. For morphological characterization, 24 descriptors were used. For molecular characterization, 11 microsatellite primers specific for sweet potatoes were used, obtaining 210 polymorphic bands. Morphological and molecular diversity was obtained by dissimilarity matrices based on the coefficient of simple matching and the Jaccard index for morphological and molecular data, respectively. From these matrices, dendrograms were built. There is a large amount of genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fitotecnia Ipomoea batatas (L.) Lam.; Morphological markers; Microsatellites; Genetic diversity; Germplasm bank. Melhoramento genético de plantas.
Ano: 2017
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/32847

Genetic variability in a population of Astyanax scabripinnis: recent bottleneck and the possible influence of individuals with B chromosomes
Provedor de dados: 3
Autores: Limeira, Daniel Meneguello; Santos, Mateus Henrique; Mateus, Rogério Pincela; Almeida, Mara Cristina de; Artoni, Roberto Ferreira.
Access the genetic variability of endangered and isolated populations has become an important conservation tool. Astyanax scabripinnis is a well-known fish model for genetic studies, forming very isolated populations in headwaters. Besides that, this species frequently presents supernumerary chromosomes, which elevates the interest on genetic studies. Genetic diversity of an Astyanax scabripinnis population from the Atlantic Forest (Serra da Mantiqueira region, Brazil) was assessed with microsatellite markers for the first time. Since microsatellite markers are not described for this species, we tested markers described for a related species for transferability to A. scabripinnis. Six polymorphic loci were sufficiently reliable for population genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allelic deficiency; Heterozygosity excess; Fixation index; Microsatellites; Supernumerary chromosomes.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/47323

Genetic diversity assessed by microsatellite markers in the amphicarpic species Trifolium polymorphum Poir.
Provedor de dados: 42
Autores: CONTERATO,IONARA F.; SCHIFINO-WITTMANN,MARIA TERESA; GUERRA,DIVANILDE; BÜTTOW,MIRIAM V.; AGNOL,MIGUEL DALL’; DAHMER,NAIR.
ABSTRACT Trifolium polymorphum Poir. is an amphicarpic forage legume from southern Brazil, Uruguay, Argentina, Paraguay and Chile. Information on the genetic diversity of natural populations in natural grasslands in southern Brazil is limited. In order to increase the knowledge about this species, an analysis of the genetic diversity was carried out in 10 natural populations of T. polymorphum with the use of 20 microsatellite markers. The expected heterozygosity in T. polymorphum populations ranged from 0.40 to 0.43, with a mean of 0.42. A total of 193 alleles were detected with a mean of 9.3 alleles per locus and polymorphic information content (PIC) for these markers of 0.62 to 0.89 with a mean of 0.84.The grouping based on the Jaccard’s coefficient of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Microsatellites; Natural grasslands; Pampa biome.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401685

Molecular marker assisted selection for increasing inbreeding in S1 populations of cassava
Provedor de dados: 42
Autores: OLIVEIRA,PAULO HENRIQUE G.A. DE; BARBOSA,ANA CLAUDIA O.; DINIZ,RAFAEL P.; OLIVEIRA,EDER J. DE; FERREIRA,CLAUDIA F.
Abstract The objective of the present work was to use MAS in self-pollinated cassava populations for obtaining individuals with high inbreeding (f) aimed at rapid development of partial inbred lines. Three progenies (F0222, F1378 and F1662) were self-pollinated, generating a total of 233 S1 individuals. The progenies and the S1 individuals were evaluated in the seedlings and clonal evaluation trials (CETs). In the CET, plants were evaluated for the following traits: plant height (PH), root dry matter content (DMC), above ground yield (AGY), root (RY) and starch yield (SY). Twenty-seven microsatellites and five minisatellites were used to determine the level of inbreeding of the S1 individuals. Inbreeding (f) values varied from 0.15 to 0.89 within...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Partial inbred lines; Manihot esculenta Crantz; Microsatellites; Self-pollination.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703853

Is the Murciano-Granadina a single goat breed? A molecular genetics approach
Provedor de dados: 45
Autores: Martínez,A.M.; Vega-Pla,J.L.; León,J.M.; Camacho,M.E.; Delgado,J.V.; Ribeiro,M.N.
The population structure of the Murciano-Granadina breed was determined using 25 microsatellites from 266 goats of seven populations. The results of the genetic differentiation analysis showed that it is possible to differentiate the Murciana and Granadina populations even though a low F ST value (0.0432) had been obtained. Individuals could be assigned to their populations with a success rate of more than 80%. Bayesian-based clustering analysis of allele frequencies and multivariate analysis revealed that Murciana and Granadina populations were grouped in different clusters since K=3. The results demonstrate that Murciana and Granadina are still two different genetic groups included into Murciano-Granadina denomination. There is the opportunity to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dairy goat; Microsatellites; Genetic diversity; Conservation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000500023

Discrepancy variation of dinucleotide microsatellite repeats in eukaryotic genomes
Provedor de dados: 48
Autores: GAO,HUAN; CAI,SHENGLI; YAN,BINLUN; CHEN,BAIYAO; YU,FEI.
To address whether there are differences of variation among repeat motif types and among taxonomic groups, we present here an analysis of variation and correlation of dinucleotide microsatellite repeats in eukaryotic genomes. Ten taxonomic groups were compared, those being primates, mammalia (excluding primates and rodentia), rodentia, birds, fish, amphibians and reptiles, insects, molluscs, plants and fungi, respectively. The data used in the analysis is from the literature published in the Journal of Molecular Ecology Notes. Analysis of variation reveals that there are no significant differences between AC and AG repeat motif types. Moreover, the number of alleles correlates positively with the copy number in both AG and AC repeats. Similar conclusions...
Tipo: Journal article
Palavras-chave: Microsatellites; Eukaryotic genomes; Variation of SSR; Copy number; Meta-analysis.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300011

Use of microsatellites for evaluation of genetic diversity in cherry tomato
Provedor de dados: 53
Autores: Aguirre,Nelson Ceballos; López,Walter; Orozco-Cárdenas,Martha; Coronado,Yacenia Morillo; Vallejo-Cabrera,Franco.
ABSTRACT Much of the diversity of tomato is found in wild forms, the most important being the Solanum lycopersicum L. var. cerasiforme and S. pimpinellifolium. The objective of this research was to assess the genetic diversity of 30 introductions of cherry tomato with 36 microsatellite molecular markers. The study was conducted at the Plant Transformation Research Center (PTRC) of the University of California. A dendrogram was built using the Dice-Nei and Li similarity index and the UPGMA clustering method, where introductions were differentiated without preserving a distribution pattern obeying the geographical area of origin. A coefficient of genetic differentiation was found (Fst = 0.3474), showing a high genetic differentiation of the introductions;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant genetic resources; Microsatellites; Real-time PCR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000200220

A comparative genetic diversity assessment of industrial and household Brazilian cassava varieties using SSR markers
Provedor de dados: 53
Autores: Siqueira,Marcos Vinicius Bohrer Monteiro; Borges,Aline; Valle,Teresa Losada; Veasey,Elizabeth Ann.
This study was carried out in order to assess the genetic diversity of 20 cassava (Manihot esculenta Crantz) genotypes with high phenotypic performance using microsatellite markers. Two groups were considered for this study: eleven varieties for industrial uses and nine for household consumption. By using nine microsatellite primers, high polymorphism was identified in all the loci analyzed, with values reaching 100%. On average, 3.4 alleles per locus were found, with 0.371 the value estimated for the observed heterozygosity and 0.555 for gene diversity for the entire set of varieties. The genetic variability found in both varieties, cultivated on a large-scale in the South Center region of Brazil, is wide enough to allow the choice of divergent parental...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Genetic diversity; Germplasm; Microsatellites.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052011000400003

Comparative analysis of genetic diversity among the maize inbred lines (Zea mays L.) obtained by RAPD and SSR markers
Provedor de dados: 52
Autores: Souza,Silvia Graciele Hülse de; Carpentieri-Pípolo,Valéria; Ruas,Claudete de Fátima; Carvalho,Valdemar de Paula; Ruas,Paulo Maurício; Gerage,Antônio Carlos.
The RAPD and SSR markers were used to compare the genetic diversity among the 16 maize inbred lines. Twenty-two primers were used in the RAPD reactions, resulting in the amplification of 265 fragments, while 16 pairs of SSR primers resulted in 75 fragments. The similarity based on Dice coefficient for the RAPD ranged from 53 to 84% and for the SSR from 11 to 82%. The dendrogram obtained by the RAPD showed five groups, while dendrogram obtained by the SSR showed three groups and one isolated line. The association constructed from the markers and the principal coordinate’s analysis separated lines into two groups according to endosperm color, either orange or yellow. The RAPD were effective to validate pedigree data, while the SSR were effective to recognize...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corn; Distance genetic; Microsatellites; Molecular markers; Polymorphism; RAPD.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100022

Genetic Variability of Brycon hilarii in a Repopulation Program
Provedor de dados: 52
Autores: Bignardi,Annaiza Braga; Povh,Jayme Aparecido; Alves,Mariana Srucki; Goes,Elenice Souza dos Reis; Filho,Ruy Alberto Caetano Corrêa; Castro,Reinaldo José; Barrero,Nelson Mauricio Lopera.
ABSTRACT Brycon hilarii, popularly called piraputanga in Brazil, is a species distributed throughout the whole basin of the river Paraguay. In recent years, the species has been on a repopulation program due to its remarkable decline as a wild species in the region. Assessment of the genetic diversity of broodstock and fingerling stocks in repopulation programs is basic to avoid genetic impacts on wild populations. The genetic variability of the wild population and of the broodstock and fingerling stocks of B. hilarii in a repopulation program in the river Itiquira MT Brazil will be determined. Seven microsatellite loci produced 52 polymorphic alleles and heterozygosity revealed rates between 0.5794 and 0.7204. FIS did not register any endogamy in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation genetics; Microsatellites; Piraputanga.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100424

Genetic variability among the wild boars (Sus scrofa scrofa), crossbred animals and pigs using microsatellite markers (STRs)
Provedor de dados: 52
Autores: Silva,Paula Viana Correa da; Lui,Jeffrey Frederico; Band,Guilherme de Oliveira; Regitano,Luciana Correia de Almeida; Grossi,Selma de Fátima; Sollero,Bruna Pena; Nunes,Cleujosí da Silva.
The aim of this work was to study the genetic variability among the wild boars, crossbred animals and pigs using microsatellite markers. Five genetic groups were studied. The fragments of three microsatellites developed for Sus scrofa domestica - IGF1, ACTG2 and TNFB - were amplified through PCR technique to evaluate the expected intra populacion variability (He) and observed (Ho) heterozygosity, and endogamy coefficient (F IS ) within each population and inter population variability F IS , testing relationship among five genetic groups to establish the genetic distance among them. The high level of observed heterozygosity values varied between 0.537 and 0.7871. Generally, F IS was low, suggesting that the endogamy did not exist between the tested animals.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular genetics; Wild boars; Microsatellites; Fragments; Amplification.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200011

Evaluation of nine microsatellite loci and misidentification paternity frequency in a population of Gyr breed bovines
Provedor de dados: 62
Autores: Curi,Rogério Abdallah; Lopes,Catalina Romero.
Paternity misidentification is harmful due to the reduction in annual genetic earnings of the population and because it endangers an efficient genetic improvement program. The objectives of the present study was to evaluate nine microsatellites in Paternity Testing and to investigate misidentification paternity frequency in families of Gyr breed bovines population. In the present experiment blood samples from forty Gir breed families ( bull / cow / calf ), registered pure breed in the Zebu Breeders Brazilian Association (ABCZ) were used. The most part of the microsatellites used in this work were recommended by the International Society of Animal Genetics (ISAG). The genomic DNA extraction was performed from whole blood samples. The microsatellites...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA; Microsatellites; Paternity; Bovine; Gyr.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000300004

Implementation of a Molecular System for Traceability of Beef Based on Microsatellite Markers
Provedor de dados: 117
Autores: Felmer D,Ricardo; Sagredo D,Boris; Chávez R,Renato; Iraira H,Sergio; Folch M,Carolina; Parra G,Lorena; Catrileo S,Adrián; Ortiz L,Manuel.
Animal products traceability has acquired considerable importance as a security measure in EEC member countries since the food crisis of the mid-nineties. This has led to reinforcing the capacity to manage cattle product quality, with traceability emerging as the main tool to prevent risks to product security and quality as demanded by consumers in developed countries. The practical application of a traceability system for beef, based on molecular markers requires the election of a panel of microsatellites, as well as the optimization of methods of sampling and DNA analysis. In this work, a traceability system for beef based on a panel of 10 microsatellites markers was implemented. Different biological samples were evaluated, such as hair, blood, tissue...
Tipo: Journal article
Palavras-chave: Traceability; Microsatellites; Individual identification; Beef.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000400004

Diversity of microsatellites linked to the FSHbeta gene, their usefulness for individual identification and association with reproductive performance
Provedor de dados: 65
Autores: Duarte,Letícia Becker Homrich; Moraes,José Carlos Ferrugem; Weimer,Tania de Azevedo.
The genetic diversity of three microsatellites (ILSTS027, MBO22, BM4325) mapped on the bovine chromosome 15 and linked to the follicle-stimulating hormone beta gene (FSHß) was investigated in cows of a Brangus Ibagé herd and the efficiency of these markers for individual identification and parentage control was estimated. Possible associations between each molecular marker and cow reproductive performance were also analyzed. Six alleles were detected in BM4325 and ILSTS027 and 12 in MB022, the most frequent being BM4325*101, BM4325*103, ILSTS027*169 and MB022*229. Polymorphic information content ranged from 0.58 to 0.88 while expected heterozygosity ranged from 65% to 89%, with an average mean value of 77%. Although only three markers were studied, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Bovine genetic diversity; Individual identification; Reproductive performance; Marker-assisted selection.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000100023

Identification of molecular markers on bovine chromosome 18 associated to calving interval in a Brangus-Ibagé cattle herd
Provedor de dados: 65
Autores: Weimer,Tania de Azevedo; Steigleder,Clara Sabina; Machado,Márcia Silveira; Almeida,Sabrina Esteves Matos; Oliveira,João Francisco Coelho; Moraes,José Carlos Ferrugem; Henkes,Luis Ernani.
In the detection phase of a bovine marker assisted selection program, this paper investigated the genetic variability of three microsatellites on the chromosome 18 (BTA 18). The possible associations between genotypes or alleles of these markers versus weight at first calving and a lifetime calving interval (as indicators of reproductive performance) were evaluated in a beef cattle herd (5/8 Aberdeen Angus x 3/8 Nelore). Eleven alleles were detected in TGLA227 and ILSTS002 and three in BMS3004, the most frequent being TGLA227*79, ILSTS002*133, ILSTS002*135 and BMS3004*129. Polymorphic information content ranged from 0.41 to 0.84, while heterozygosity ranged from 49% to 86%, with an average value of 77%. The association analyses performed between genotype...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Reproduction; Marker assisted selection; BTA 18.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000500050

Simulation of pattern of gene flow in Canjerana fragments in the Brazilian Atlantic Rainforest for evaluating genetic conservation strategies
Provedor de dados: 65
Autores: Serrote,Caetano Miguel Lemos; Reiniger,Lia Rejane Silveira; Costa,Leonardo Severo da; Stefanel,Charlene Moro; Silva,Karol Buuron da; Rabaiolli,Silvia Machado dos Santos.
ABSTRACT: Gene flow is important for the conservation of genetic resources to allow connectivity of geographically isolated populations and which genetic variability is reduced. Gene movement is a function of flow rate and model. Understanding how gene flow occurs can contribute to the conservation and selection of priority populations that could benefit from an eventual intervention. Simulation softwares allow making inferences about past events based on current datasets or predict future phenomena under real genetic scenarios. Adverse phenomena can be predicted and actions can be taken to avoid them. The aim of this study was to identify a model and the gene flow rates that could explain genetic structure of eight forest fragments of Cabralea canjerana...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cabralea canjerana; Reproductive isolation; Genetic variability; EASYPOP; Microsatellites.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000800301

Quantitative trait loci associated with resistance to gray leaf spot and grain yield in corn
Provedor de dados: 64
Autores: Veiga,Adriano Delly; Pinho,Renzo Garcia Von; Resende,Luciane Vilela; Pinho,Édila Vilela de Resende Von; Balestre,Márcio; Pereira,Laís Andrade.
The main objectives of hybrid development programs include incorporating genetic resistance to diseases and increasing grain yield. Identification of Quantitative Trait Loci (QTL) through the statistical analysis of molecular markers allows efficient selection of resistant and productive hybrids. The objective of this research was to identify QTL associated with resistance to gray leaf spot and for grain yield in the germplasm of tropical corn. We used two strains with different degrees of reaction to the disease; the genotypes are owned by GENESEEDS Ltda, their F1 hybrid and the F2 population. The plants were evaluated for gray leaf spot resistance, for grain yield and were genotyped with 94 microsatellite markers. Association of the markers with the QTL...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maximum likelihood; Microsatellites; Regression.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000100004

Identification of haploid maize by flow cytometry, morphological and molecular markers
Provedor de dados: 64
Autores: Couto,Evellyn Giselly de Oliveira; Davide,Livia Maria Chamma; Bustamante,Fernanda de Oliveira; Pinho,Renzo Garcia Von; Silva,Tallyta Nayara.
The development of homozygous breeding lines in maize may be accelerated through the use of haploids. Thus, the obtaining and prior identification of haploids generated by the haploid inducer lines is an important factor. The purpose of this study was to identify haploids by flow cytometry and to correlate the nuclear DNA content to the morphological and morphometric traits of the seeds that gave rise to them. In addition, molecular markers were used to confirm the androgenetic nature of the haploid. The seeds obtained were derived from the cross between the inbred line W23 and the commercial hybrid P30F90. Among these seeds, a group was selected, putative haploids, whose embryo was white and the pericarp purplish. This group, consisting of 330 seeds, was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays; Nuclear DNA content; Androgenetic inheritance; R-navajo; Microsatellites.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542013000100003