Consulta: subjectFacets:"Molecular cytogenetics"
Registros recuperados: 9
Data/hora: 02/06/2024 07:10:06

Genomic imbalances in esophageal squamous cell carcinoma identified by molecular cytogenetic techniques
Provedor de dados: 74
Autores: Bellini,Marilanda Ferreira; Silva,Ana Elizabete; Varella-Garcia,Marileila.
This review summarizes the chromosomal changes detected by molecular cytogenetic approaches in esophageal squamous cell carcinoma (ESCC), the ninth most common malignancy in the world. Whole genome analyses of ESCC cell lines and tumors indicated that the most frequent genomic gains occurred at 1, 2q, 3q, 5p, 6p, 7, 8q, 9q, 11q, 12p, 14q, 15q, 16, 17, 18p, 19q, 20q, 22q and X, with focal amplifications at 1q32, 2p16-22, 3q25-28, 5p13-15.3, 7p12-22, 7q21-22, 8q23-24.2, 9q34, 10q21, 11p11.2, 11q13, 13q32, 14q13-14, 14q21, 14q31-32, 15q22-26, 17p11.2, 18p11.2-11.3 and 20p11.2. Recurrent losses involved 3p, 4, 5q, 6q, 7q, 8p, 9, 10p, 12p, 13, 14p, 15p, 18, 19p, 20, 22, Xp and Y. Gains at 5p and 7q, and deletions at 4p, 9p, and 11q were significant prognostic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CGH; Esophageal carcinoma; FISH; Genomic imbalances; Molecular cytogenetics.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200001

Astyanax hastatus Myers, 1928 (Teleostei, Characidae): a new species complex within the genus Astyanax?
Provedor de dados: 74
Autores: Kavalco,Karine Frehner; Brandão,Karina de Oliveira; Pazza,Rubens; Almeida-Toledo,Lurdes Foresti de.
Four populations of Astyanax hastatus Myers 1928 from the Guapimirim River basin (Rio de Janeiro State) were analyzed and three distinct cytotypes identified. These cytotypes presented 2n = 50 chromosomes, with 4M+8SM+10ST+28A (Cytotype A), 8M+10SM+14ST+18A (Cytotype B), 6M+8SM+4ST+32A (Cytotype C) and scanty heterochromatin, mainly located throughout pericentromeric regions of several chromosomal pairs. No homologies with the As-51 satellite DNA were observed in the three cytotypes, although all of them presented multiple 18S rDNA sites, as detected by both silver nitrate staining and FISH (fluorescent in situ hybridization). The application of the term "species complex" in Astyanax is discussed from a cytotaxonomic viewpoint.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astyanax hastatus; Molecular cytogenetics; Karyotypic evolution; Species complex.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300009

Human molecular cytogenetics: from cells to nucleotides
Provedor de dados: 74
Autores: Riegel,Mariluce.
The field of cytogenetics has focused on studying the number, structure, function and origin of chromosomal abnormalities and the evolution of chromosomes. The development of fluorescent molecules that either directly or via an intermediate molecule bind to DNA has led to the development of fluorescent in situ hybridization (FISH), a technology linking cytogenetics to molecular genetics. This technique has a wide range of applications that increased the dimension of chromosome analysis. The field of cytogenetics is particularly important for medical diagnostics and research as well as for gene ordering and mapping. Furthermore, the increased application of molecular biology techniques, such as array-based technologies, has led to improved resolution,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular cytogenetics; FISH; Array-CGH; Copy number variation; Genomic disorders.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200006

The lowest diploid number in Testudines: Banding patterns, telomeric and 45S rDNA FISH in Peltocephalus dumerilianus, 2n = 26 and FN = 52 (Pleurodira, Podocnemididae)
Provedor de dados: 74
Autores: Ventura,Karen; Moreira,Camila N; Moretti,Renata; Yonenag-Yassuda,Yatiyo; Rodrigues,Miguel T.
The karyotype of the big-headed Amazon River turtle, Peltocephalus dumerilianus, is characterized based on a sample of seven juveniles from Reserva Biológica do Rio Trombetas, Pará State, Brazil (1°30' S, 56°34' W). Here we present the first results on GTG and CBG-banding patterns, Ag-NOR staining and FISH, with telomeric and 45S rDNA sequences as probes. A cytogenetic comparison with related Podocnemidae is also provided.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Big-headed Amazon River turtle; Molecular cytogenetics; Pelomedusoides.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100011

Chromosomal polymorphism and comparative chromosome painting in the rufous-collared sparrow (Zonotrichia capensis)
Provedor de dados: 74
Autores: Bülau,Sandra Eloisa; Kretschmer,Rafael; Gunski,Ricardo José; Garnero,Analía del Valle; O’Brien,Patricia C. M.; Ferguson-Smith,Malcolm A.; Oliveira,Edivaldo Herculano Correa de; Freitas,Thales Renato Ochotorena de.
Abstract Zonotrichia capensis is widely distributed in the Neotropics. Previous cytogenetic studies demonstrated the presence of polymorphisms in two chromosome pairs (ZCA2 and ZCA4). Here, we report results based on comparative chromosome painting, using probes derived from Gallus gallus and Leucopternis albicollis, focused on characterizing the chromosome organization of Z. capensis. Our results demonstrate the conservation of ancestral syntenies as observed previously in other species of passerine. Syntenies were rearranged by a series of inversions in the second chromosome as described in other Passeriformes, but in this species, by using probes derived from L. albicollis we observed an extra inversion in the second chromosome that had not previously...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Birds; Chromosomal rearrangements; Molecular cytogenetics; FISH.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500799

Comparative analyses of three swallow species (Aves, Passeriformes, Hirundinidae): Insights on karyotype evolution and genomic organization
Provedor de dados: 74
Autores: Barcellos,Suziane Alves; Kretschmer,Rafael; Souza,Marcelo Santos de; Costa,Alice Lemos; Degrandi,Tiago Marafiga; Lopes,Cassiane Furlan; Ferguson-Smith,Malcolm A.; Pereira,Jorge; Oliveira,Edivaldo Herculano Correa de; Gunski,Ricardo José; Garnero,Analía del Valle.
Abstract Despite the richness of species in the Hirudinidae family, little is known about the genome organization of swallows. The Progne tapera species presents genetic and morphological difference when compared to other members of the same genus. Hence, the aims of this study were to analyze the chromosomal evolution of three species Progne tapera, Progne chalybea and Pygochelidon cyanoleuca - by comparative chromosome painting using two sets of probes, Gallus gallus and Zenaida auriculata, in order to determine chromosome homologies and the relationship between these species. All karyotypes exhibited 76 chromosomes with similar morphology, except for the 5th, 6th and 7th chromosome pairs in P. cyanoleuca. Additionally, comparative chromosome painting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Homology; Molecular cytogenetics; Fluorescent in situ hybridization; Hirundinidae.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100201

Cytogenetic mapping of the ALMT (aluminum-activated malate transporter) gene in wheat genotypes
Provedor de dados: 63
Autores: Oliveira,Érica Cristina de; Pinto-Maglio,Cecília Alzira Ferreira.
ABSTRACT: Presence of the ALMT1 (aluminum-activated malate transporter) gene confers resistance to aluminum toxicity in Triticum aestivum (common wheat). No resistant cultivars of Triticum turgidum ssp. Durum Desf. (durum wheat) have been registered in Brazil. The aim of this study was to map the ALMT1 through application of the FISH (fluorescence in situ hybridization) technique in five wheat genotypes, common and durum, from the Active Germplasm Bank (AGB) of the Instituto Agronômico (IAC): BH 1146, P19, P33, Anahuac, and IAC 1003. FISH-ALMT1 signals were registered in Anahuac (sensitive) chromosomes and in BH 1146, P19, and P33 (resistant) chromosomes. In the three resistant genotypes, a characteristic double FISH signal was found, located in different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triticum sp.; Molecular cytogenetics; Fluorescence in situ hybridization; Aluminum tolerance; Toxicity.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000501102

rDNA mapping, heterochromatin characterization and AT/GC content of Agapanthus africanus (L.) Hoffmanns (Agapanthaceae)
Provedor de dados: 42
Autores: REIS,ARYANE C.; FRANCO,ANA LUIZA; CAMPOS,VICTÓRIA R.; SOUZA,FLÁVIA R.; ZORZATTO,CRISTIANE; VICCINI,LYDERSON F.; SOUSA,SAULO M.
ABSTRACT Agapanthus (Agapanthaceae) has 10 species described. However, most taxonomists differ respect to this number because the great phenotypic plasticity of the species. The cytogenetic has been an important tool to aid the plant taxon identification, and to date, all taxa of Agapanthus L'Héritier studied cytologically, presented 2n = 30. Although the species possess large chromosomes, the group is karyologically little explored. This work aimed to increase the cytogenetic knowledge of Agapanthus africanus (L.) Hoffmanns by utilization of chromosome banding techniques with DAPI / CMA3 and Fluorescent in situ Hybridization (FISH). In addition, flow cytometry was used for determination of DNA content and the percentage of AT / GC nitrogenous bases....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: African lily; Chromosome banding; Cytotaxonomy; Flow cytometry; Genome size; Molecular cytogenetics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501727

Cytogenetics of coffee
Provedor de dados: 61
Autores: Pinto-Maglio,Cecília A. F.
The genus Coffea L. has around 100 native species distributed in tropical and subtropical areas in Africa, and the most important economic species are C. arabica and C. canephora. C. arabica is exceptional in the genus since it is the only species so far analyzed which is self-compatible, and a natural polyploid with 2n=4x=44 chromosomes; it is considered to be a segmental allopolyploid because it presents a disomic inheritance and a regular meiotic behavior. All other species in the genus are self-incompatible diploids with 2n=2x=22. Cytogenetic studies in Coffea, undertaken since 1912, have followed various phases: initial studies were limited only to establishing chromosome counts. Subsequent studies characterized the karyotypes of various species using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea; Chromosomal characterization; Karyotype; Meiotic behavior; Chromomere pattern; Chromosome banding; Molecular cytogenetics.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100004