Consulta: subjectFacets:"Molecular detection"
Registros recuperados: 6
Data/hora: 10/06/2024 04:45:49

Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds
Provedor de dados: 95
Autores: Barrocas,Ellen Noly; Machado,José da Cruz; Almeida,Mirella Figueiró de; Botelho,Luana Silva; Pinho,Édila Vilela de Resende Von.
Maize seeds, infected by Stenocarpella species, are important sources of inoculum for the introduction and dissemination of stalk and ear rot and macrospore leaf spot diseases. The use of healthy seeds is an important strategy for the preventive control of these diseases. However, one of the difficulties in the health quality control programs for maize seeds is the availability of a reliable and quick method for detecting these fungi during routine seed analyses. Therefore, the objective of the present study was to investigate the possibility of using the PCR technique as an alternative method for accurately detecting these pathogens in maize seed samples. Maize seeds were kept in contact with S. maydis colonie developed in PDA media containing mannitol at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fungus; Molecular detection; Stalk and ear rot; Macrospore leaf spot.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000200005

Protocol optimization for the detection of Trypanosoma cruzi DNA in açai (Euterpe oleraceae) pulp
Provedor de dados: 36
Autores: CARDOSO,Gabrielle Virgínia Ferreira; OLIVEIRA,Andrey Carlos do Sacramento de; SILVA,Andréia Silva da; SILVA,Marcos Clécio de Lemos; LIMA,Joelson Sousa; ROOS,Talita Bandeira; MORAES,Carina Martins de.
ABSTRACT Chagas disease, caused by the protozoan Trypanosoma cruzi, has often been linked to oral transmission through açai consumption. Molecular methods that allow fast and accurate identification of the pathogen are important for the detection of the presence of the parasite in this food. This study aimed to optimize polymerase chain reaction (PCR)-based detection of T. cruzi DNA in açai pulp. Several dilutions of T. cruzi DTU TcI trypomastigote forms were cultured in liver infusion tryptose (LIT) medium. Trypanosoma cruzi DNA was extracted from the cells and subjected to PCR. Subsequently, culture dilutions were added to açai pulp to evaluate the detection threshold of the optimized PCR assay. We demonstrate that our assay can detect T. cruzi DNA in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Detection method; Foodborne disease; Food pathogen; Molecular detection; Chagas disease.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672021000100079

Analysis of drug resistance mutations in pulmonary Mycobacterium tuberculosis isolates in the Southern coastal region of Andhra Pradesh, India
Provedor de dados: 55
Autores: Prasad,Polu Giri; Jasmine,Mohammad Shaik; kanta,Kota Neela Mani; Deepthi,Karumanchi; Allam,Uday Sankar.
ABSTRACT  Purpose and objectives Detection of drug resistance plays a crucial role in tuberculosis (TB) treatment and prevention of Mycobacterium tuberculosis (MTB) transmission. The aim of this study was to determine the levels and patterns of resistance of MTB isolates to two key anti-TB drugs (rifampicin, RIF and isoniazid, INH) and the type of mutations in drug resistance genes (rpoB, katG and inhA) of the isolates at the southern coastal region of Andhra Pradesh, India, using commercially available GenoType MTBDRplus assay under the Revised National TB Control Program.  Methods GenoType MTBDRplus assay was performed on 2859 sputum smear-positive samples and the mutations in the genes responsible for resistance (rpoB, katG and inhA) were analyzed....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multidrug resistance; Mycobacterium tuberculosis; MTBDRplus assay; Mutations; Molecular detection.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000500281

Molecular detection of Anaplasma platys in a naturally-infected cat in Brazil
Provedor de dados: 58
Autores: Lima,M.L.F; Soares,P.T; Ramos,C.A.N; Araújo,F.R; Ramos,R.A.N; Souza,I.I.F; Faustino,M.A.G; Alves,L.C.A.
Following the accidental finding of inclusion bodies similar to Anaplasma platys in a stained blood smear from a cat, DNA analysis of the 16S rRNA gene was performed and 100% identity was found with different strains of A. platys. These data confirm that cats are susceptible to parasitism by A. platys.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feline; Anaplasma platys; Molecular detection.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200019

Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA
Provedor de dados: 58
Autores: Santos,Carla R.; Franciscatto,Laura G.; Barcellos,Regina B.; Almeida,Sabrina E. M; Rossetti,Maria Lucia R.
This study aimed to evaluate the use of the FTA elute cardTM impregnated with cervicovaginal sample directly in the PCR amplification for detection of HPV-DNA. The results were compared to a reference technique. This method was more efficient than the protocol indicated by the manufacturer, identifying 91.7% against 54.2% of the positive samples.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPV; Cervical cancer; FTA elute; Molecular detection.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100047

Genotyping of South American clinical isolates of Pythium insidiosum based on single nucleotide polymorphism-based multiplex PCR
Provedor de dados: 65
Autores: Weiblen,Carla; Azevedo,Maria Isabel de; Ianiski,Lara Baccarin; Stibbe,Paula Cristina; Pereira,Daniela Isabel Brayer; Zanette,Régis Adriel; Sangioni,Luís Antônio; Rivero,Rodolfo; Santurio,Janio Morais; Botton,Sônia de Avila.
ABSTRACT: We aimed to genotype the South American clinical isolates of Pythium insidiosum using the single nucleotide polymorphisms (SNP) of the ribosomal DNA sequences (rDNA). Previously, an SNP-based multiplex-PCR was able to distinguish three different clades of P. insidiosum isolates. Thus, we used this assay to evaluate South American clinical isolates of P. insidiosum (n=32), standard strains from Costa Rica (n=4), Thailand (n=3), Japan (n=1), and India (n=1), a standard strain of Pythium aphanidermatum, and Brazilian environmental isolates of Pythium torulosum, Pythium rhizo-oryzae and Pythium pachycaule voucher (n=3). It was possible to allocate each American P. insidiosum isolate to clade I, the isolates of India, Japan, and Thailand to clade II,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pythium insidiosum; Pythiosis; Molecular detection; Genotype; Single nucleotide polimorphisms.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000100450