Consulta: subjectFacets:"Molecular diagnosis"
Registros recuperados: 28
Data/hora: 06/06/2024 02:51:21

Costs of genetic testing: Supporting Brazilian Public Policies for the incorporating of molecular diagnostic technologies
Provedor de dados: Genetics and Molecular Biology
Autores: Schlatter,Rosane Paixão; Matte,Ursula; Polanczyk,Carisi Anne; Koehler-Santos,Patrícia; Ashton-Prolla,Patricia.
This study identifies and describes the operating costs associated with the molecular diagnosis of diseases, such as hereditary cancer. To approximate the costs associated with these tests, data informed by Standard Operating Procedures for various techniques was collected from hospital software and a survey of market prices. Costs were established for four scenarios of capacity utilization to represent the possibility of suboptimal use in research laboratories. Cost description was based on a single site. The results show that only one technique was not impacted by rising costs due to underutilized capacity. Several common techniques were considerably more expensive at 30% capacity, including polymerase chain reaction (180%), microsatellite instability...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular diagnosis; Hereditary cancer; Cost analysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300332

Molecular analysis of the most prevalent mutations of the FANCA and FANCC genes in Brazilian patients with Fanconi anaemia
Provedor de dados: Genetics and Molecular Biology
Autores: Rodriguez,David Enrique Aguilar; Lima,Carmen Silvia Passos; Lourenço,Gustavo Jacob; Figueiredo,Maria Estela; Carneiro,Jorge David Aivazoglu; Tone,Luiz Gonzaga; Llerena Jr.,Juan Clinton; Toscano,Raquel Alves; Brandalise,Silvia; Pinto Júnior,Walter; Costa,Fernando Ferreira; Bertuzzo,Carmen Sílvia.
Fanconi anaemia (FA) is a recessive autosomal disease determined by mutations in genes of at least eleven complementation groups, with distinct distributions in different populations. As far as we know, there are no reports regarding the molecular characterisation of the disease in unselected FA patients in Brazil. OBECTIVE: This study aimed to investigate the most prevalent mutations of FANCA and FANCC genes in Brazilian patients with FA. METHODS: Genomic DNA obtained from 22 racially and ethnically diverse unrelated FA patients (mean age ± SD: 14.0 ± 7.8 years; 10 male, 12 female; 14 white, 8 black) was analysed by polymerase chain reaction and restriction site assays for identification of FANCA (delta3788-3790) and FANCC (delta322G, IVS4+4A -&gt; T,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fanconi anaemia; DEB test; Molecular diagnosis; FANCA; FANCC.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200004

Variants of the HNF1α gene: a molecular approach concerning diabetic patients from southern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Bonatto,Naieli; Nogaroto,Viviane; Svidnicki,Paulo V.; Milléo,Fábio Q.; Grassiolli,Sabrina; Almeida,Mara C.; Vicari,Marcelo R.; Artoni,Roberto F.
Maturity Onset Diabetes of the Young (MODY) presents monogenic inheritance and mutation factors which have already been identified in six different genes. Given the wide molecular variation present in the hepatocyte nuclear factor-1α gene (HNF1α) MODY3, the aimof this study was to amplify and sequence the coding regions of this gene in seven patients from the Campos Gerais region, Paraná State, Brazil, presenting clinical MODY3 features. Besides the synonymous variations, A15A, L17L, Q141Q, G288G and T515T, two missense mutations, I27L and A98V, were also detected. Clinical and laboratory data obtained from patients were compared with the molecular findings, including the I27L polymorphism that was revealed in some overweight/obese diabetic patients of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MODY3; Molecular diagnosis; Diabetes mellitus; Nucleotide sequencing.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500005

Application of a Genus-Specific LAMP Assay for Schistosome Species to Detect Schistosoma haematobium x Schistosoma bovis Hybrids
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Crego-vicente, Beatriz; Fernández-soto, Pedro; Febrer-sendra, Begoña; García-bernalt Diego, Juan; Boissier, Jérôme; Angora, Etienne K.; Oleaga, Ana; Muro, Antonio.
Schistosomiasis is a disease of great medical and veterinary importance in tropical and subtropical regions caused by different species of parasitic flatworms of the genus Schistosoma. The emergence of natural hybrids of schistosomes indicate the risk of possible infection to humans and their zoonotic potential, specifically for Schistosoma haematobium and S. bovis. Hybrid schistosomes have the potential to replace existing species, generate new resistances, pathologies and extending host ranges. Hybrids may also confuse the serological, molecular and parasitological diagnosis. Currently, LAMP technology based on detection of nucleic acids is used for detection of many agents, including schistosomes. Here, we evaluate our previously developed...
Tipo: Text
Palavras-chave: LAMP; Schistosomiasis; Schistosome hybrids; Schistosoma haematobium; Schistosoma bovis; Molecular diagnosis; Species-specific LAMP; Genus-specific LAMP.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00686/79840/82659.pdf

Characterization of the protozoan parasite Marteilia refringens infecting the dwarf oyster Ostrea stentina in Tunisia
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Elgharsalli, Refka; Aloui-bejaoui, Nejla; Sala, Hedi; Chollet, Bruno; Joly, Jean-pierre; Robert, Maeva; Couraleau, Yann; Arzul, Isabelle.
Marteilia refringens is a protozoan parasite recognized as a significant pathogen of the European flat oyster Ostrea edulis. The life cycle of this species is still poorly known, although there is evidence of the need for intermediate host(s). In the present study, we have used molecular approaches to identify this parasite in samples of the dwarf oyster Ostrea stentina after reports of massive mortality along the Tunisian coasts. In 2009 we evaluated the status of O. stentina from Monastir and checked if there was an infection with M. refringens, using polymerase chain reaction assays. Of the 103 tested O. stentina, 85 were PCRpositive using a Marteilia genus-specific assay. Additional assays were subsequently carried out on some samples collected in 2010...
Tipo: Text
Palavras-chave: Marteilia refringens; Molecular diagnosis; IGS; ITS; Transmission electron microscopy; Ostrea stentina.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00124/23538/21422.pdf

Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Paiva-Cavalcanti,M; Regis-da-Silva,CG; Gomes,YM.
In recent years, the polymerase chain reaction (PCR) technique has significantly advanced towards expanding its use and versatility by working with quantitative real-time PCR (qPCR). Data from the literature show that both methods present interesting characteristics for the diagnosis of visceral leishmaniasis. The benefits of qPCR in relation to conventional PCR include speed, reproducibility and quantitative ability. In addition to operational advantages, qPCR is more sensitive and reproducible and may replace conventional PCR in diagnostic routines. Regarding visceral leishmaniasis, the possibility of deployment of real-time PCR in highly complex diagnoses (reference services) in endemic areas will facilitate a swift and safe return for patients....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; Molecular diagnosis; Visceral leishmaniasis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400004

Identification of the capsule type of Pasteurella multocida isolates from cases of fowl cholera by multiplex PCR and comparison with phenotypic methods
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Furian,TQ; Borges,KA; Pilatti,RM; Almeida,C; Nascimento,VP do; Salle,CTP; Moraes,HL de S.
The ability of Pasteurella multocida to invade and multiply in its host is enhanced by the presence of the capsule, one of the most important virulence factors for this bacterium. Capsular typing methods are often used in epidemiological and pathogenesis studies of this agent. Five different serogroups have been identified based on serological typing. However, such tests are laborious, and agglutination of homologous antiserum may fail. The aim of this study was to develop a multiplex PCR protocol for the identification of the hyaD-hyaC and dcbF genes specific to serogroups A and D, respectively, and to compare these results with those of phenotypic tests for 54 strains isolated from fowl cholera cases in southern Brazil. The kappa coefficient and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular diagnosis; Non-serologic tests; Pasteurellosis; Serogroup.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000200004

MOLECULAR DIAGNOSIS OF Guignardia citricarpa IN ASYMPTOMATIC SWEET ORANGE TISSUE
Provedor de dados: Revista Brasileira de Fruticultura
Autores: FAGANELLO,FERNANDA DE SILLOS; CARRER FILHO,RENATO; DIAS,VANESSA DUARTE; MORELLO,REGINA MELO SARTORI COELHO; CUNHA,MARCOS GOMES DA.
ABSTRACT  Citrus black spot, a fungal disease caused by the quarantine fungus Guignardia citricarpa, restricts the exportation of fresh fruit to countries in the European Union. The occurrence of latent infections and the time required for diagnosis using conventional methods have brought about the need to validate fast, efficient and reproducible molecular techniques to detect the pathogen in asymptomatic tissue. As such, this study aims to detect G. citricarpa in the symptomatic fruit and asymptomatic leaf tissue of sweet oranges by conventional and real-time polymerase chain reaction (PCR). Specificity and limit of detection (LOD) were assessed in tissue samples of fruit lesions and asymptomatic leaves. Low concentrations of the fungus were found in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citriculture; Molecular diagnosis; Phyllosticta citricarpa.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000400404

Selection of aggressive pathogenic and solopathogenic strains of Ustilago maydis to improve Huitlacoche production
Provedor de dados: Acta Botanica Brasilica
Autores: Galicia-García,Porfirio Raúl; Silva-Rojas,Hilda Victoria; Mendoza-Onofre,Leopoldo Ernesto; Zavaleta-Mancera,Hilda Araceli; Córdova-Téllez,Leobigildo; Espinosa-Calderón,Alejandro.
ABSTRACT Ustilago maydis is a basidiomycete known as the causative agent of 'common smut', worldwide disease of maize that is recognized by the galls it forms, which have considerable potential as a gourmet food. Results of infection are quite variable, even under optimal greenhouse conditions. In order to find pathogenic strains able to be used as a highly infective and stable inoculum for the successful production of galls either in greenhouses or in the field, ears with gall symptoms containing teliospores were recovered from maize plants. The teliospores were suspended in water and plated on nutrient-rich medium. Twenty-six colonies developed, containing three types of yeast-like colonies: saprotrophic, pathogenic, and solopathogenic. DAPI staining...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Basidiomycete; Maize galls; Mating types; Molecular diagnosis; Smut.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062016000400683

Improvement of the specific detection of Xanthomonas phaseoli pv. manihotis based on the pthB gene
Provedor de dados: Acta Scientiarum: Agronomy
Autores: Cerqueira Melo, Rita de Cássia; Dorea Bragança, Carlos Augusto; Nogueira Pestana, Katia; Silva, Harllen Sandro Alves da; Ferreira, Claudia Fortes; Alves Santos de Oliveira, Saulo.
Modifications were made in the PCR conditions aiming to overcome the problem of non-amplification of the Xanthomonas phaseoli pv. manihotis (Xpm) fragment, using the primer pair XV / XK described in the literature. The objective of this study was to propose changes in the primers already described (XV / XK_MOD) and validate the use of these new primers in identifying Xpm. The validation procedure was carried out with the primer pair XV and XK_MOD, using different strains of Xpm, other plant pathogenic and endophytic bacteria genera and one isolate of X. phaseoli pv. passiflorae. As a control, additional reactions were conducted in multiplex with the universal primers for the 16S rRNA gene of the bacteria together with XV / XK and XV / XK_MOD. Using the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xpm; Cassava bacterial blight; Molecular diagnosis; Resistance.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42708

Molecular investigation of Neorickettsia risticii in trematodes and snails in a region with serological evidence of this agent in horses, state of Rio de Janeiro
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Costa,R.L.; Vitari,G.L.V.; Silva,C.B.; Peckle,M.P.; Pires,M.S.; Brandolini,S.V.P.B.; Pinheiro,J.; Massard,C.L.; Santos,H.A.
ABSTRACT In Brazil, some studies have indicated that Neorickettsia risticii circulates in horses, but it is unclear which are the possible intermediate vectors of this bacterium in the country. The aim of this study was to use molecular techniques in order to analyze the presence of N. risticii in snails and larval stages of trematodes in farms in a region with a history of seroreactive horses towards this bacterium, in Rio de Janeiro, Brazil. Convenience sampling was used in the studied region. The collected snails were exposed to incandescent light (60W) for 2-4 hours in order to investigate trematodes in larval forms. Deoxyribonucleic acid (DNA) was extracted from snail tissue and trematode. Real-time PCR (qPCR) technique was used to investigate the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Equine neorickettsiosis; Molecular diagnosis; QPCR; Intermediate hosts.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000601470

Clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Paraná, Brazil
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Thomaz-Soccol,Andréa; Mocellin,Marcos; Mulinari,Fabiane; Castro,Edilene Alcântara de; Queiroz-Telles,Flávio de; Alcântara,Fernanda de Souza; Bavaresco,Marcello Tortelli; Hennig,Luciane; Andraus,Anielo; Luz,Ennio; Thomaz-Soccol,Vanete.
The aim of the present work was to study the clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Parana, Brazil. Twenty one suspected cases of mucocutaneous leishmaniasis (MCL) in patients from the endemic areas of leishmaniasis were assessed. Different methods used in diagnosing the disease and the polymerase chain reaction (PCR) technique were compared in order to establish the sensitivity of each method. Out of the 21 patients analyzed, 14.3% presented other etiologies such as vasculitis, syphilis, and paracoccidioidomycosis, with all tests negative for leishmaniasis. Out of the remaining 15 patients, 6.7% cases were confirmed for leishmaniasis by direct examination; 46.67% were positive for culture,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucocutaneous leishmaniasis; Molecular diagnosis; Leishmania (Viannia) braziliensis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300008

Effectiveness of the BDProbeTec ET system for detection of Mycobacterium tuberculosis complex in sputum and bronchoalveolar lavage specimens
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Hung,Hung-Chang; Chan,Chi-Ho; Tsao,Shih-Ming; Chen,Shiuan-Chih; Wu,Tzu-Chin; Lu,Min-Chi; Wu,Der-Jinn; Huang,Shu-Ju; Lee,Yuan-Ti.
OBJECTIVE: The diagnostic efficacy of the BDProbeTEC ET Mycobacterium tuberculosis (MTB) complex direct detection assay (DTB) performed on bronchoalveolar lavage (BAL) specimens and sputum smears was compared with acid-fast bacilli (AFB) smear microscopy. METHOD: AFB smear microscopy, DTB and culture results of 286 patients with pulmonary tuberculosis were retrospectively reviewed. A total of 120 patients provided expectorated sputum samples, and 166 patients provided BAL specimens. Culture results and clinical diagnosis were used as gold standards. RESULTS: The sensitivity and specificity of the DTB assay in detecting MTB in sputum specimens was significantly higher compared to AFB smear microscopy (83.7% and 82.4%, vs. 75.6%, and 41.2%, respectively)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bronchoalveolar lavage; Mycobacterium tuberculosis; Acid-fast bacilli staining; Molecular diagnosis; BDProbeTEC ET system.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000300005

Molecular diagnosis of cerebral toxoplasmosis: comparing markers that determine Toxoplasma gondii by PCR in peripheral blood from HIV-infected patients
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Mesquita,Rafael Tonini; Vidal,José Ernesto; Pereira-Chioccola,Vera Lucia.
As cerebral toxoplasmosis is the most common cerebral focal lesion in AIDS patients, this study evaluated three PCR markers for diagnosis, since some limitations remain present, such as low parasite levels in some clinical samples. The molecular markers were B22-B23 and Tg1-Tg2 (based on the B1 gene) and Tox4-Tox5 (non-coding fragment, repeated 200-300-fold). DNA samples from 102 AIDS patients with previously known diagnosis were analyzed. The cerebral toxoplasmosis group was constituted of DNA extracted from the blood of 66 AIDS patients, which was collected before or until the third day of the therapy for toxoplasmosis. DNA from the blood of 36 AIDS patients with other neurologic opportunistic infections was used as control group. Sensitivities of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AIDS; Cerebral toxoplasmosis; Molecular diagnosis; PCR markers.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000400006

Rothia aeria endocarditis in a patient with a bicuspid aortic valve: case report
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Nicodemo,Antonio Carlos; Gonçalves,Luiz Guilherme; Odongo,Fatuma Catherine Atieno; Martino,Marines Dalla Valle; Sampaio,Jorge Luiz Mello.
Rothia aeria is an uncommon pathogen mainly associated with endocarditis in case reports. In previous reports, endocarditis by R. aeria was complicated by central nervous system embolization. In the case we report herein, endocarditis by R. aeria was diagnosed after acute self-limited diarrhea. In addition to the common translocation of R. aeria from the oral cavity, we hypothesize the possibility of intestinal translocation. Matrix-assisted laser desorption ionization-time of flight mass spectrometry and genetic sequencing are important tools that can contribute to early and more accurate etiologic diagnosis of severe infections caused by Gram-positive rods.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Rothia aeria; Endocarditis; Intestinal translocation; Molecular diagnosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000500561

Validation and clinical application of a molecular method for the identification of Cryptococcus neoformans/Cryptococcus gattii complex DNA in human clinical specimens
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Rivera,Vanessa; Gaviria,Marcela; Muñoz-Cadavid,Cesar; Cano,Luz; Naranjo,Tonny.
ABSTRACT The diagnosis of cryptococcosis is usually performed based on cultures of tissue or body fluids and isolation of the fungus, but this method may require several days. Direct microscopic examination, although rapid, is relatively insensitive. Biochemical and immunodiagnostic rapid tests are also used. However, all of these methods have limitations that may hinder final diagnosis. The increasing incidence of fungal infections has focused attention on tools for rapid and accurate diagnosis using molecular biological techniques. Currently, PCR-based methods, particularly nested, multiplex and real-time PCR, provide both high sensitivity and specificity. In the present study, we evaluated a nested PCR targeting the gene encoding the ITS-1 and ITS-2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryptococcus neoformans/Cryptococcus gattiicomplex; Nested PCR; Cryptococcosis; Molecular diagnosis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600563

Validation and clinical application of a nested PCR for paracoccidioidomycosis diagnosis in clinical samples from Colombian patients
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Gaviria,Marcela; Rivera,Vanessa; Muñoz-Cadavid,Cesar; Cano,Luz Elena; Naranjo,Tonny Williams.
Paracoccidioidomycosis is a systemic and endemic mycosis, restricted to tropical and subtropical areas of Latin America. The infection is caused by the thermal dimorphic fungus Paracoccidioides brasiliensisand Paracoccidioides lutzii. The diagnosis of paracoccidioidomycosis is usually performed by microscopic examination, culture and immunodiagnostic tests to respiratory specimens, body fluids and/or biopsies; however these methods require laboratory personnel with experience and several days to produce a result. In the present study, we have validated and evaluated a nested PCR assay targeting the gene encoding the Paracoccidioides gp43membrane protein in 191 clinical samples: 115 samples from patients with proven infections other than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paracoccidioides brasiliensis; Nested PCR; Paracoccidioidomycosis; Molecular diagnosis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000400376

Direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry and real-time PCR in a combined protocol for diagnosis of bloodstream infections: a turnaround time approach
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Quiles,Milene Gonçalves; Boettger,Bruno Cruz; Inoue,Fernanda Matsiko; Monteiro,Jussimara; Santos,Daniel Wagner; Ponzio,Vinicius; Carlesse,Fabianne; Cappellano,Paola; Carvalhaes,Cecilia Godoy; Pignatari,Antonio Carlos Campos.
ABSTRACT Bloodstream infections (BSIs) are serious infections associated with high rates of morbidity and mortality. Every hour delay in initiation of an effective antibiotic increases mortality due to sepsis by 7%. Turnaround time (TAT) for conventional blood cultures takes 48 h, forcing physicians to streamline therapy by exposing patients to broad-spectrum antimicrobials. Our objective was (1) to evaluate the accuracy and TAT of an optimized workflow combining direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and in-house real-time polymerase chain reaction (PCR) for bacterial identification and antimicrobial resistance profiling directly from positive blood bottles for diagnosing bloodstream infections...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bloodstream infections; Molecular diagnosis; Molecular panels; PCR; Mass spectrometry.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000300164

Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples
Provedor de dados: Brazilian Journal of Microbiology
Autores: Cruz,Heidi Lacerda Alves da; Montenegro,Rosana de Albuquerque; Lima,Juliana Falcão de Araújo; Poroca,Diogo da Rocha; Lima,Juliana Figueirêdo da Costa; Montenegro,Lílian Maria Lapa; Crovella,Sergio; Schindler,Haiana Charifker.
The polymerase chain reaction (PCR) and its variations, such as the nested-PCR, have been described as promising techniques for rapid diagnosis of tuberculosis (TB). With the aim of evaluating the usefulness of a nested-PCR method on samples of blood and urine of patients suspected of tuberculosis we analyzed 192 clinical samples, using as a molecular target the insertion element IS6110 specific of M. tuberculosis genome. Nested-PCR method showed higher sensitivity in patients with extrapulmonary tuberculosis (47.8% and 52% in blood and urine) when compared to patients with the pulmonary form of the disease (sensitivity of 29% and 26.9% in blood and urine), regardless of the type of biological sample used. The nested-PCR is a rapid technique that, even if...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Nested-PCR; Blood; Urine; Molecular diagnosis; IS6110 insertion sequence.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100041

Detection of Mycobacterium in clinical samples by multiprimer polymerase chain reaction
Provedor de dados: Brazilian Journal of Microbiology
Autores: Barouni,Abdulmonam Saied; Saridakis,Halha Ostrensky; Vidotto,Marilda Carlos.
We investigated the use of multiprimer-PCR for detection of mycobacteria species in clinical samples. Three different mycobacterial genomic fragments were investigated: the IS6110 insertion sequence, present in M. tuberculosis complex; the genus specific fragment (32kDa); and from M. tuberculosis species-specific mtp40 gene. The sensitivity and specificity using 135 clinical isolates were 94.5% and 95.9%, respectively, compared with culture in Löwenstein-Jensen medium; the detection limit was 0.05ng of DNA. In conclusion, this assay is reliable and rapid for detection of Mycobacterium species in clinical samples, and differentiates M. tuberculosis from M. bovis strains in a single-step assay.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Multiprimer-PCR; Molecular diagnosis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100004

Epidemiological aspects of astrovirus and coronavirus in poults in the South Eastern Region of Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Silva,S.E.L. da; Bonetti,A.M.; Petrocelli,A.; Ferrari,H.F.; Luvizotto,M.C.R.; Cardoso,T.C.
A survey of Turkey Coronavirus (TCoV) and Astrovirus (TAstV-2) prevalence was carried out from February to December during 2006 year in semiarid region of Brazil, from a turkey producer area, localized in South Eastern of Brazil. To asses the risk factor related to clinical material, climatic condition and type of RT-PCR applied, cloacal swabs (CS), faeces, sera, bursa of Fabricius (BF), thymus (TH) and spleen (SP) and ileum-caeca region were collected from 30-day-old poults suffering of enteritis episode characterized as poult enteritis mortality syndrome (PEMS). The PEMS clinical features were characterized by watery to foamy faeces, light brown-yellow in colour and low mortality rate. Meteorological data (rainfall and relative humidity) observed during...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Turkey Astrovirus; Turkey Coronavirus; Molecular diagnosis; PEMS.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200008

Rapid diagnosis of vesicular stomatitis virus in Ecuador by the use of polymerase chain reaction
Provedor de dados: Brazilian Journal of Microbiology
Autores: Sepúlveda,Lya Madureira; Malirat,Viviana; Bergmann,Ingrid E.; Mantilla,Anibal; Nascimento,Elmiro Rosendo do.
Vesicular Stomatitis (VS) is a viral disease that has a great impact in animal health, as infected animals present marked decrease in meat and milk production. Its presence is a limiting factor for international animal trade. Besides the damage in the livestock productivity, such disease assumes an important role in animal health programs since it is clinically indistinguishable from Foot-and-Mouth Disease. The diagnosis of the VS has been made, mainly, through Complement Fixation, ELISA and Virus Neutralization tests, assays that allow not only for viral detection but also for differentiation of the two serotypes described for Vesicular Stomatitis Virus (VSV): New Jersey (NJ) and Indiana (Ind). In this work, a molecular diagnostic approach, the polymerase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymerase Chain reaction; Vesicular Stomatitis; Molecular diagnosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300022

Application of broad-range bacterial PCR amplification and direct sequencing on the diagnosis of neonatal sepsis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Ruppenthal,Rubia Denise; Pereira,Fabiana de Souza; Cantarelli,Vlademir Vicente; Schrank,Irene Silveira.
A broad-range bacterial PCR target to conserved regions of the 23S rDNA was applied to 306 blood culture samples from 295 infants (up to one year of age) admitted to a neonatal intensive care unit. Classic blood culture results were compared to DNA sequencing analysis of the PCR amplification products. Culture results were in agreement to DNA sequencing in 90.5% (277) of 306 samples tested, including 263 PCR and culture negative samples and 29 culture and PCR positive samples. The sensitivity of the PCR method combined with sequencing was 88%, and the specificity was 96.3%, with positive and negative predictive values of 74.3 e 98.5%, respectively. The PCR-based approach directly applied to blood culture samples, correlated well with blood culture results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sepsis; Molecular diagnosis; Neonatal; PCR.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100006

Clinical and epidemiological use of nested PCR targeting the repetitive element IS1111 associated with the transposase gene from Coxiella burnetii
Provedor de dados: Brazilian Journal of Microbiology
Autores: Mares-Guia,Maria Angélica M.M.; Guterres,Alexandro; Rozental,Tatiana; Ferreira,Michelle dos Santos; Lemos,Elba R.S.
ABSTRACT Q fever is a worldwide zoonosis caused by Coxiella burnetii—a small obligate intracellular Gram-negative bacterium found in a variety of animals. It is transmitted to humans by inhalation of contaminated aerosols from urine, feces, milk, amniotic fluid, placenta, abortion products, wool, and rarely by ingestion of raw milk from infected animals. Nested PCR can improve the sensitivity and specificity of testing while offering a suitable amplicon size for sequencing. Serial dilutions were performed tenfold to test the limit of detection, and the result was 10× detection of C. burnetti DNA with internal nested PCR primers relative to trans-PCR. Different biological samples were tested and identified only in nested PCR. This demonstrates the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Q fever; Coxiella burnetii; Molecular diagnosis; Nested PCR; IS1111.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100138

Canine distemper virus detection by different methods of One-Step RT-qPCR
Provedor de dados: Ciência Rural
Autores: Tozato,Claudia de Camargo; Zadra,Vívian Ferreira; Basso,Caroline Rodrigues; Araújo Junior,João Pessoa.
ABSTRACT: Three commercial kits of One-Step RT-qPCR were evaluated for the molecular diagnosis of Canine Distemper Virus. Using the kit that showed better performance, two systems of Real-time RT-PCR (RT-qPCR) assays were tested and compared for analytical sensitivity to Canine Distemper Virus RNA detection: a One-Step RT-qPCR (system A) and a One-Step RT-qPCR combined with NESTED-qPCR (system B). Limits of detection for both systems were determined using a serial dilution of Canine Distemper Virus synthetic RNA or a positive urine sample. In addition, the same urine sample was tested using samples with prior centrifugation or ultracentrifugation. Commercial kits of One-Step RT-qPCR assays detected canine distemper virus RNA in 10 (100%) urine samples from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canine distemper; Molecular diagnosis; One-Step RT-qPCR; Urine; Centrifugation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901601

Pneumonia by Cryptococcus neoformans in a goat in the Southern region of Brazil
Provedor de dados: Ciência Rural
Autores: Bianchi,Ronaldo Michel; Cecco,Bianca Santana de; Schwertz,Claiton Ismael; Panziera,Welden; Andrade,Caroline Pinto de; Spanamberg,Andreia; Ravazzolo,Ana Paula; Ferreiro,Laerte; Driemeier,David.
ABSTRACT:  This report describes the clinical, pathological, and molecular aspects of a pneumonia by Cryptococcus neoformans in a goat in the Southern region of Brazil. A goat with a history of prolonged transportation presented dyspnea, nasal discharge and cough, and was subjected to necropsy, histopathology, and mycological evaluation. Grossly, cranio-ventral pulmonary consolidation was observed, characterized by firm and grayish areas interspersed with dark red foci. Histopathology of the lungs showed areas of parenchymal necrosis, containing blastoconidia with a slightly basophilic central cell, surrounded by an unstained capsule. It was associated with moderate granulomatous inflammatory infiltrate and peripheral fibrosis. The capsule and fungus cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Goats; Respiratory diseases; Pathology; Mycology; Molecular diagnosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001000551

Fatal systemic Mortierella wolfii infection in a neonatal calf in southern Brazil
Provedor de dados: Ciência Rural
Autores: Bianchi,Ronaldo Michel; Vielmo,Andréia; Schwertz,Claiton Ismael; Cabreira,Adan; Rocha,Jéssica Goulart da; Siqueira,Franciele Maboni; Pavarini,Saulo Petinatti.
ABSTRACT: This study described the pathological and microbiological aspects of a fatal systemic Mortierella wolfii infection in a neonatal calf in southern Brazil. The calf was born clinically normal, but on the third day of life it presented apathy, unilateral hypopyon, and neurological signs, and in the next day it was euthanized. At necropsy, multiple soft, and white-yellow nodules were observed in the liver, spleen, kidneys, mesenteric lymph nodes, heart, and lungs. In the brain, reddish, and friable areas were found. In the eye, there were anterior and posterior synechiae, diffuse thickening of choroid, and anterior chamber filled by whitish and friable material. Microscopically, areas of necrosis, pyogranulomatous inflammation, vasculitis,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle diseases; Fungal diseases; Pathology; Molecular diagnosis.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000100551

Oral infection of neonate gerbils by Neospora caninum tachyzoites
Provedor de dados: Ciência Rural
Autores: Ferreira,Maiara Sanitá Tafner; Vogel,Fernanda Silveira Flores; Sangioni,Luis Antonio; Weber,Augusto; Bräunig,Patricia; Vaz,Marcos André Braz; Cezar,Alfredo Skrebsky.
ABSTRACT: Neosporosis is a parasitic disease caused by the protozoan Neospora caninum which results in major economic losses for cattle breeding due to abortion and other reproductive disorders. Gerbils (Meriones unguiculatus) are commonly used as experimental models for neosporosis due to their high susceptibility to N. caninum infection, both by oocysts ingestion as by tachyzoites/bradyzoites parenteral inoculation. However, the risk of transmission by tachyzoites ingestion is not fully elucidated. In this study, infection of neonate gerbils by N. caninum (NC-1 strain) tachyzoites inoculated by the oral route and the parasite distribution in gerbils' tissues were evaluated by protozoan DNA detection. Seventeen neonate gerbils, aged 4-5 days, were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular diagnosis; Experimental model; Bovine neosporosis; Rodents.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400654