Consulta: subjectFacets:"Molecular epidemiology"
Registros recuperados: 26
Data/hora: 10/06/2024 14:08:31

Polymorphisms associated with the risk of lung cancer in a healthy Mexican Mestizo population: application of the additive model for cancer
Provedor de dados: 74
Autores: Pérez-Morales,Rebeca; Méndez-Ramírez,Ignacio; Castro-Hernández,Clementina; Martínez-Ramírez,Ollin C.; Gonsebatt,María Eugenia; Rubio,Julieta.
Lung cancer is the leading cause of cancer mortality in Mexico and worldwide. In the past decade, there has been an increase in the number of lung cancer cases in young people, which suggests an important role for genetic background in the etiology of this disease. In this study, we genetically characterized 16 polymorphisms in 12 low penetrance genes (AhR, CYP1A1, CYP2E1, EPHX1, GSTM1, GSTT1, GSTPI, XRCC1, ERCC2, MGMT, CCND1 and TP53) in 382 healthy Mexican Mestizos as the first step in elucidating the genetic structure of this population and identifying high risk individuals. All of the genotypes analyzed were in Hardy-Weinberg equilibrium, but different degrees of linkage were observed for polymorphisms in the CYP1A1 and EPHX1 genes. The genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Additive model; Genetic polymorphism; Lung cancer; Mexicans; Molecular epidemiology.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400003

Distribution of N-acetyltransferase Type 1 (NAT1) genotypes and alleles in a Turkish population
Provedor de dados: 74
Autores: Arslan,Serdal; Degerli,Naci; Bardakci,Fevzi.
NAT1 is an intronless gene on chromosome 8p21.3 encoding a 290-amino-acid-long protein showing acetyltransferase activity. Some 26 alleles of NAT1 gene have been identified in human populations. In the present study we determined the distributions of NAT1 genotypes and alleles in a sample of 201 individuals from the Turkish population in Central Anatolia. The most frequent genotypes were NAT1*4/NAT1*4 (51.74%), NAT1*10/NAT1*4 (22.39%), NAT1*11/NAT1*4 (7.46), NAT1*10/NAT1*10 (3.98%). Frequencies of NAT1*3, *4 (wild-type), *10 and *11 alleles were 3.73%, 69.6%, 17.66% and 7.2%, respectively. The frequency of NAT1*11 was the highest amongst the populations studied so far, the other allele frequencies being close to those described in Caucasian populations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NAT1 gene; Genetic polymorphism; Molecular epidemiology; Turkish population.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200005

Lack of detection of human papillomavirus DNA in prostate carcinomas in patients from northeastern Brazil
Provedor de dados: 74
Autores: Araujo-Neto,Ari P.; Ferreira-Fernandes,Hygor; Amaral,Carolina M.M.; Santos,Lina G.; Freitas,Antônio C.; Silva-Neto,Jacinto C.; Rey,Juan A.; Burbano,Rommel R.; Silva,Benedito B. da; Yoshioka,France K.N.; Pinto,Giovanny R.
Abstract Prostate cancer is the second most common cancer among men in western populations, and despite its high mortality, its etiology remains unknown. Inflammatory processes are related to the etiology of various types of tumors, and prostate inflammation, in particular, has been associated with prostate cancer carcinogenesis and progression. Human papillomavirus (HPV) is associated with benign and malignant lesions in the anogenital tract of both females and males. The possible role of HPV in prostate carcinogenesis is a subject of great controversy. In this study, we aimed to examine the prevalence of HPV infections in prostate carcinomas of patients from northeastern Brazil. This study included 104 tissue samples from primary prostate carcinoma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Prostate cancer; Prevalence; HPV PCR detection; Molecular epidemiology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100024

Contamination of Clams with Human Norovirus and a Novel Hepatitis A Virus in Cameroon
Provedor de dados: 5
Autores: Bonny, Patrice; Desdouits, Marion; Schaeffer, Julien; Garry, Pascal; Essia Ngang, Jean Justin; Le Guyader, Soizick.
Shellfish constitute an important protein source but may be contaminated by viruses from various origins. A study performed on clams collected in Cameroon showed a high prevalence of norovirus and hepatitis A virus. After sequencing, the hepatitis A virus showed similarities with the genotype V simian strains. The need for a one-health approach to protect humans from emerging diseases is evident by the high number of microbial pathogens that become zoonotic following insect bites or consumption of contaminated meat. This is particularly well documented for infectious disease transmission from non-human primates to human (Devaux et al. 2019). Environmental issues are critical in such events, especially for RNA viruses that may be excreted at high...
Tipo: Text
Palavras-chave: Shellfish; Hepatitis A virus; Molecular epidemiology.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00635/74745/74851.pdf

Molecular evolution and phylogeography of infectious hematopoietic necrosis virus with a focus on its presence in France over the last 30 years
Provedor de dados: 5
Autores: Bellec, Laure; Louboutin, Lenaig; Cabon, Joelle; Castric, Jeanne; Cozien, Joelle; Thiery, Richard; Morin, Thierry.
Infectious hematopoietic necrosis virus (IHNV) is among the most important pathogens affecting the salmonid industry. Here, we investigated the molecular evolution and circulation of isolates from 11 countries or regions all over the world, with a special focus on the epidemiological situation in France. The phylogeography, time to the most recent common ancestor (TMRCA) and nucleotide substitution rate were studied using 118 full-length glycoprotein gene sequences isolated from 9 countries (5 genogroups) over a period of 47 years. The TMRCA dates back to 1943, with the L genogroup identified as the likely root (67 %), which is consistent with the first report of this pathogen in the USA. A Bayesian inference approach was applied to the partial...
Tipo: Text
Palavras-chave: Infectious hematopoietic necrosis virus; Evolutionary dynamics; Dates of divergence; Phylogeography; Molecular epidemiology.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00399/51015/51832.pdf

Prevalence, Molecular Identification, and Risk Factors for Cryptosporidium Infection in Edible Marine Fish: A Survey Across Sea Areas Surrounding France
Provedor de dados: 5
Autores: Certad, Gabriela; Follet, Jérôme; Gantois, Nausicaa; Hammouma-ghelboun, Ourida; Guyot, Karine; Benamrouz-vanneste, Sadia; Fréalle, Emilie; Seesao, Yuwalee; Delaire, Baptiste; Creusy, Colette; Even, Gaël; Verrez-bagnis, Veronique; Ryan, Una; Gay, Mélanie; Aliouat-denis, Cécile; Viscogliosi, Eric.
Cryptosporidium, a zoonotic pathogen, is able to infect a wide range of hosts including wild and domestic animals, and humans. Although it is well known that some parasites are both fish pathogens and recognized agents of zoonosis with a public health impact, little information is available concerning the prevalence of Cryptosporidium in wild aquatic environments. To evaluate the prevalence of Cryptosporidium spp. in commercially important edible marine fish in different European seas (English channel, North sea, Bay of Biscay, Celtic sea and Mediterranean sea), 1,853 specimens were collected as part of two surveys. Nested PCR followed by sequence analysis at the 18S rRNA gene locus was used to identify Cryptosporidium spp. The overall prevalence of...
Tipo: Text
Palavras-chave: Cryptosporidium; Edible marine fish; 18S rRNA gene; Gp60; Molecular epidemiology; Phylogeny; Novel genotypes; European seas.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00497/60864/64198.pdf

Molecular techniques for the study and diagnosis of parasite infection
Provedor de dados: 80
Autores: Tavares,RG; Staggemeier,R; Borges,ALP; Rodrigues,MT; Castelan,LA; Vasconcelos,J; Anschau,ME; Spalding,SM.
In parasitology, routine laboratory diagnosis involves conventional methods, such as optical microscopy, used for the morphological identification of parasites. Currently, molecular biology techniques are increasingly used to diagnose parasite structures in order to enhance the identification and characterization of parasites. The objective of the present study was to review the main current and new diagnostic techniques for confirmation of parasite infections, namely: polymerase chain reaction (PCR), real-time polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), Luminex xMAP, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and restriction fragment length polymorphism (RFLP), in addition...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parasite infection; Diagnosis; Molecular techniques; Molecular epidemiology.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300003

GENETIC DIVERSITY OF Lutzomyia (Nyssomyia) intermedia IN AN ENDEMIC AREA OF AMERICAN CUTANEOUS LEISHMANIASIS, STATE OF PARANÁ, BRAZIL
Provedor de dados: 37
Autores: GONÇALVES,André Luiz; CASTRO,Edilene Alcântara de; LUZ,Ennio; Cancio FENDRICH,Ricardo; Araújo de SOUZA,Nataly; THOMAZ-SOCCOL,Vanete.
ABSTRACT Lutzomyia intermedia (Diptera: Psychodidae) features as one of the main vectors that are involved in the transmission of American cutaneous leishmaniasis (ACL) in the Neotropical region. However, genetic studies involving this taxon are still incipient and important for understanding the level of variability of different populations, their role, and implications as vectors. The aim of this study was to determine the level of genetic diversity of L. intermedia present in the Ribeira River Valley, an area of ACL transmission in the state of Paraná, Brazil, through the Random amplified polymorphic DNA (RAPD). Two municipalities were chosen to collect sand flies: Cerro Azul (new transmission area of the ACL) and Adrianópolis (endemic area of the ACL)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intraspecific variation; Molecular epidemiology; Phenetic analysis; Phlebotominae.
Ano: 2021
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2021000300365

Determinants of HIV-1 mother-to-child transmission in Southern Brazil
Provedor de dados: 42
Autores: Martínez,Ana M.B.; Hora,Vanusa P. da; Santos,Adriana L. dos; Mendoza-Sassi,Raul; Von Groll,Andréa; Soares,Esmeralda A.J.M.; D'Ávila,Nildo; Silveira,Jussara; Leal,Renata G.; Tanuri,Amilcar; Soares,Marcelo A.
Different human immunodeficiency virus type 1 (HIV-1) subtypes may have distinct biological, immunological and pathogenic properties. Efficiency of mother-to-child transmission (MTCT) may be among those properties, but few and controversial results have been described so far. In this study, 102 children born from HIV-1-infected mothers between 1998 and 2004 in the city of Rio Grande, Brazil were analyzed for potential risk factors associated with MTCT. That geographic region is characterized by a high proportion of subtype C-infected subjects, and it allowed comparison between subtypes B and C and their influence on MTCT. The analysis also included clinical, obstetric and immunological parameters. Multivariate regression analyses were conducted to evaluate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; MTCT; Vertical transmission; Subtype C; Molecular epidemiology.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000100011

Molecular diagnosis of Hepatozoon canis in symptomatic dogs in the city of Goiania, Goiás, Brazil
Provedor de dados: 45
Autores: Duarte,S.C.; Parente,J.A.; Silveira Neto,O.J.; Jayme,V.S.; Bastos,T.S.A.; Linhares,G.F.C.
ABSTRACT More than 300 species have been described in the genus Hepatozoon, occurring in different vertebrates. Among these, only Hepatozoon canis and Hepatozoon americanum are seen in dogs. Different methods may be used for laboratory diagnosis. The most common of these is direct parasitological examination of parasite stages in blood smears. The aim of this investigation was to conduct a phylogenetic study on Hepatozoon isolates from symptomatic dogs in the city of Goiânia, Goiás, Brazil. Blood samples were obtained from 40 symptomatic dogs that had been referred to the Veterinary Hospital of the Federal University of Goiás. Among these, only two samples were positive for Hepatozoon spp. using the direct parasitological method. These samples were then...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canis familiaris; Hepatozoonosis; Molecular epidemiology; Phylogeny.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000601431

Human T cell lymphotropic virus type 1 infection among men who have sex with men in Central Brazil
Provedor de dados: 55
Autores: Castro,Lisie Souza; Rezende,Grazielli Rocha de; Fernandes,Fernanda Rodas Pires; Bandeira,Larissa Melo; Puga,Marco Antonio Moreira; Tanaka,Tayana Serpa Ortiz; Weis-Torres,Sabrina Moreira dos Santos; Vicente,Ana Carolina Paulo; Otsuki,Koko; Motta-Castro,Ana Rita Coimbra.
ABSTRACT Human T cell lymphotropic virus type 1 (HTLV-1) was the first retrovirus discovered in humans and is endemic in several parts of the world. Because of risk behaviors, mainly sexual, men who have sex with men (MSM) are at high risk of acquiring HTLV-1 infection. A cross-sectional study was performed to estimate the prevalence of HTLV-1 infection, to characterize genetically HTLV-1 sequences and to identify risk behaviors associated with this infection among MSM in Central Brazil. A total of 430 MSM were enrolled in this study and three were shown to be HTLV-1 infected, prevalence of 0.7% (95% confidence interval: 0.4-0.9). Phylogenetic analysis showed that all HTLV-1 positive samples belonged to Cosmopolitan subtype Transcontinental subgroup A....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HTLV-1; Men who have sex with men; Sexual risk behavior; Molecular epidemiology; Brazil.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000600472

Factors associated with epidemic multiresistant Pseudomonas aeruginosa infections in a hospital with AIDS-predominant admissions
Provedor de dados: 55
Autores: Gomes,Marisa Zenaide Ribeiro; Oliveira,Raquel Vasconcellos C. de; Machado,Carolina Romero; Conceição,Magda de Souza da; Souza,Cristina Vieira de; Lourenço,Maria Cristina da Silva; Asensi,Marise Dutra.
INTRODUCTION: Infections caused by multiresistant Pseudomonas aeruginosa (MR-PA) have been associated with persistent infections and high mortality in acquired immunodeficiency syndrome (AIDS) patients. Therefore, understanding the predisposing factors for infection/colonization by this agent is critical for controlling outbreaks caused by MR-PA in settings with AIDS patients. OBJECTIVEAND METHODS: To analyze the presence of factors associated with the acquisition of an epidemic MR-PA strain in a hospital with AIDS-predominant admission. A case-control study was carried out in which cases and controls were gathered from a prospective cohort of all hospitalized patients in an infectious disease hospital during a five-year study period. RESULTS: Multivariate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas aeruginosa; Risk factors; Disease outbreaks; Molecular epidemiology; Acquired immunodeficiency syndrome.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000300001

Spread of multidrug-resistant Acinetobacter baumannii and Pseudomonas aeruginosa clones in patients with ventilator-associated pneumonia in an adult intensive care unit at a university hospital
Provedor de dados: 55
Autores: Royer,Sabrina; Faria,Ana Luiza Souza; Seki,Liliane Miyuki; Chagas,Thiago Pavoni Gomes; Campos,Paola Amaral de; Batistão,Deivid William da Fonseca; Asensi,Marise Dutra; Gontijo Filho,Paulo P.; Ribas,Rosineide Marques.
Background:In Brazil, ventilator-associated pneumonia (VAP) caused by carbapenem resis- tant Acinetobacter baumanniiand Pseudomonas aeruginosaisolates are associated with signiﬁcant mortality, morbidity and costs. Studies on the clonal relatedness of these isolates could lay the foundation for effective infection prevention and control programs.Objectives: We sought to study the epidemiological and molecular characteristics of A. baumannii vs. P. aeruginosaVAP in an adult intensive care unit (ICU).Methods: It was conducted a cohort study of patients with VAP caused by carbapenem resistant A. baumanniiand P'. aeruginosaduring 14 months in an adult ICU. Genomic studies were used to investigate the clonal relatedness of carbapenem resistant OXA-23-producing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acinetobacter baumannii; Intensive care unit; Molecular epidemiology; Multidrug-resistant.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000400350

The changing pattern of methicillin-resistant staphylococcus aureus clones in Latin America: implications for clinical practice in the region
Provedor de dados: 55
Autores: Rodríguez-Noriega,Eduardo; Seas,Carlos.
Methicillin-resistant Staphylococcus aureus (MRSA) clones belonging to the Brazilian, Pediatric, Cordobes/Chilean and New York/Japan clonal complexes are widely distributed across Latin America, although their individual distribution patterns and resistance to antimicrobial drugs are constantly changing. Furthermore, clones with increased virulence are beginning to appear more frequently both in hospital and community settings, and there is evidence that virulence factors can be transferred between hospital- and community-associated clones through recombination. These changing patterns have significant implications for clinical practice in the region. Most importantly, clinicians need to be aware of the changing antimicrobial resistance profile of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; Clones; Molecular epidemiology; Latin America.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000800004

Outbreaks, persistence, and high mortality rates of multiresistant Pseudomonas aeruginosa infections in a hospital with AIDS-predominant admissions
Provedor de dados: 55
Autores: Gomes,Marisa Zenaide Ribeiro; Machado,Carolina Romero; Conceição,Magda de Souza da; Ortega,Jois Alves; Neves,Sonia Maria Ferraz M; Lourenço,Maria Cristina da Silva; Asensi,Marise Dutra.
INTRODUCTION: Authors have reported increased incidence of multiresistant Pseudomonas aeruginosa (MR-PA) infections worldwide over the last decade. Researchers have proposed multifaceted approaches to control MR-PA infections, but none have been reported in the acquired immunodeficiency syndrome (AIDS) setting. OBJECTIVE AND METHODS: Herein we report the impact of a multifaceted intervention for controlling MR-PA over five years in a hospital with AIDS-predominant admissions and describe the clinical characteristics of MR-PA infection in our patient population. The clinical outcomes of infected patients and molecular characteristics of the isolated strains were used as tools for controlling MR-PA infection rates. RESULTS: Significant temporary decrease of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas aeruginosa; Disease outbreaks; Infection control; Molecular epidemiology; Acquired immunodeficiency syndrome.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400003

Virus isolation and genotype identification of human respiratory syncytial virus in Guizhou Province, China
Provedor de dados: 55
Autores: Zhao,Ting; Ye,Zhixu; Wang,Binlin; Cui,Yuxia; Nie,Yingjie; Yang,Bin; Chen,Kun; Zhang,Hua; Hu,Fangfang; Yu,Fuxun.
ABSTRACT To investigate the genetic variation and molecular epidemiology characteristics of Human Respiratory Syncytial Virus (HRSV) in Guizhou Province, nasopharyngeal aspirates were collected from patients with acute respiratory infection (ARI) in Guizhou Provincial People's Hospital, from December 2017 to March 2018, and inoculated to Hep-2 cells to isolate HRSV. Cells that showed cytopathic effect (CPE) were then confirmed by indirect immunofluorescence assay and reverse transcription. The sequence of the PCR products was determined for HRSV isolates, and the genetic variation was analyzed. Out of 196 nasopharyngeal aspirate samples, HRSV were isolated in 39. The second hypervariable region at the 3' terminal of glycoprotein gene (HVR2) sequence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human respiratory syncytial virus; Virus isolation; RT-PCR; HVR2; Phylogenetic analysis; Molecular epidemiology.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000600427

Molecular techniques for MRSA typing: current issues and perspectives
Provedor de dados: 55
Autores: Trindade,P. A.; McCulloch,J. A.; Oliveira,G. A.; Mamizuka,E. M.
Staphylococcus aureus has long been recognised as an important pathogen in human disease. Serious staphylococcal infections can frequently occur in inpatients and may lead to dire consequences, especially as to therapy with antimicrobial agents. The increase in the frequency of Methicillin-Resistant Staphylococcus aureus (MRSA) as the causal agent of nosocomial infection and the possibility of emergence of resistance to vancomycin demands a quick and trustworthy characterization of isolates and identification of clonal spread within hospitals. Enough information must be generated to permit the implementation of appropriate measures for control of infection, so that outbreaks can be contained. Molecular typing techniques reviewed in this manuscript include:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Molecular epidemiology; Genotyping; Methicillin resistance.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000100005

An outbreak of Acinetobacter baumannii septicemia in a neonatal intensive care unit of a university hospital in Brazil
Provedor de dados: 55
Autores: Brito,Denise von Dolinger de; Oliveira,Elias José; Abdallah,Vânia O. Steffen; Darini,Ana Lúcia da Costa; Gontijo Filho,Paulo P.
We studied an outbreak of two multi-drug resistant clones of Acinetobacter baumannii in the Neonatal Intensive Care Unit of the Uberlândia Federal University Hospital in Minas Gerais state, Brazil, and we analyzed the contribution of cross-transmission in the rise in infection rates. Eleven neonates who developed multi-drug resistant A. baumannii nosocomial infection were matched to 22 neonates who were admitted to the same unit and did not develop an infection during the outbreak period, in order to identify risk factors for infection. Three out of the 11 neonates died. Epidemiological investigation included molecular typing, using pulsed field gel electrophoresis. Prior to the outbreak, from December 2001 to March 2002, no case of infection by this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acinetobacter baumannii; Molecular epidemiology; Outbreak; Neonates.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000400006

Desmodus rotundus and Artibeus spp. bats might present distinct rabies virus lineages
Provedor de dados: 55
Autores: Fahl,Willian Oliveira; Carnieli Jr,Pedro; Castilho,Juliana Galera; Carrieri,Maria Luiza; Kotait,Ivanete; Iamamoto,Keila; Oliveira,Rafael Novaes; Brandão,Paulo Eduardo.
In Brazil, bats have been assigned an increasing importance in public health as they are important rabies reservoirs. Phylogenetic studies have shown that rabies virus (RABV) strains from frugivorous bats Artibeus spp. are closely associated to those from the vampire bat Desmodus rotundus, but little is known about the molecular diversity of RABV in Artibeus spp. The N and G genes of RABV isolated from Artibeus spp. and cattle infected by D. rotundus were sequenced, and phylogenetic trees were constructed. The N gene nucleotides tree showed three clusters: one for D. rotundus and two for Artibeus spp. Regarding putative N amino acid-trees, two clusters were formed, one for D. rotundus and another for Artibeus spp. RABV G gene phylogeny supported the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies Bat Artibeus spp.; Desmodus rotundus; Phylogeny; Molecular epidemiology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600008

HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil
Provedor de dados: 56
Autores: Rossini,M.A.A.; Diaz,R.S.; Caseiro,M.; Turcato,G.; Accetturi,C.A.; Sabino,E.C.
In order to assess the molecular epidemiology of HIV-1 in two neighboring cities located near the epicenter of the HIV-1 epidemics in Brazil (Santos and São Paulo), we investigated 83 HIV-1 strains obtained from samples collected in 1995 from intravenous drug users. The V3 through V5 region of the envelope of gp 120 was analyzed by heteroduplex mobility analysis. Of the 95 samples, 12 (12.6%) were PCR negative (6 samples from each group); low DNA concentration was the reason for non-amplification in half of these cases. Of the 42 typed cases from São Paulo, 34 (81%, 95% confidence limits 74.9 to 87.0%) were B and 8 (19%, 95% confidence limits 12.9 to 25.0%) were F, whereas of the 41 typed cases from Santos, 39 (95%, 95% confidence limits 91.6 to 98.4%)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HIV-1 subtypes; Intravenous drug users; Molecular epidemiology; Heteroduplex mobility analysis; Brazil.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100005

Epidemiological studies in the information and genomics era: experience of the Clinical Genome of Cancer Project in São Paulo, Brazil
Provedor de dados: 56
Autores: Wünsch-Filho,V.; Eluf-Neto,J.; Lotufo,P.A.; da Silva Jr.,W.A.; Zago,M.A.
Genomics is expanding the horizons of epidemiology, providing a new dimension for classical epidemiological studies and inspiring the development of large-scale multicenter studies with the statistical power necessary for the assessment of gene-gene and gene-environment interactions in cancer etiology and prognosis. This paper describes the methodology of the Clinical Genome of Cancer Project in São Paulo, Brazil (CGCP), which includes patients with nine types of tumors and controls. Three major epidemiological designs were used to reach specific objectives: cross-sectional studies to examine gene expression, case-control studies to evaluate etiological factors, and follow-up studies to analyze genetic profiles in prognosis. The clinical groups included...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multicenter studies; Large-scale studies; Molecular epidemiology; Data control quality; Cancer epidemiological studies.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400016

Use of molecular epidemiology to monitor the nosocomial dissemination of methicillin-resistant Staphylococcus aureus in a University Hospital from 1991 to 2001
Provedor de dados: 56
Autores: Beretta,A.L.R.Z.; Trabasso,P.; Stucchi,R.B.; Moretti,M.L.
Methicillin-resistant Staphylococcus aureus (MRSA) has been the cause of major outbreaks and epidemics among hospitalized patients, with high mortality and morbidity rates. We studied the genomic diversity of MRSA strains isolated from patients with nosocomial infection in a University Hospital from 1991 to 2001. The study consisted of two periods: period I, from 1991 to 1993 and period II from 1995 to 2001. DNA was typed by pulsed-field gel electrophoresis and the similarity among the MRSA strains was determined by cluster analysis. During period I, 73 strains presented five distinctive DNA profiles: A, B, C, D, and E. Profile A was the most frequent DNA pattern and was identified in 55 (75.3%) strains; three closely related and four possibly related...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methicillin-resistant Staphylococcus aureus; Molecular epidemiology; Hospital infection; Pulsed-field gel electrophoresis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900009

Genetic variability of hepatitis A virus isolates in Rio de Janeiro: implications for the vaccination of school children
Provedor de dados: 56
Autores: Villar,L.M.; Lampe,E.; Meyer,A.; Gaspar,A.M.C.
The epidemiology of hepatitis A virus (HAV) infection is shifting from high to intermediate endemicity in Brazil, resulting in increased numbers of susceptible individuals and a greater potential for the emergence of outbreaks. Universal vaccination against HAV has been recommended for children, but updated sero-epidemiological data are necessary to analyze the level of natural immunity and to identify candidates for preventive measures. In addition, more molecular studies are necessary to characterize the genotypes involved in HAV infections and outbreaks. Sera from 299 school children (5-15 years old) and 25 school staff members, collected during an outbreak of HAV at a rural public school in June 2000, were tested for IgM and total anti-HAV antibodies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis A virus (HAV); PCR; Molecular epidemiology; Sero-epidemiology; Vaccination.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200003

Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil
Provedor de dados: 56
Autores: Villar,L.M.; Morais,L.M.; Aloise,R.; Melo,M.M.M.; Calado,I.A.; Lampe,E.; Gaspar,A.M.C.
The Northeast region is the location of most cases of acute hepatitis A virus (HAV) in Brazil. In the present study, the genotypes of HAV strains from Pernambuco State, one of most populous states in the Northeast region, were characterized. Blood samples positive for anti-HAV IgM from 145 individuals (mean age = 29.1 years), collected during 2002 and 2003, were submitted to nested RT-PCR for amplification of the 5'non-translated region (5'NTR) and VP1/2A regions of the HAV genome. The VP1/2A and 5'NTR regions were amplified in 39 and 21% of the samples, respectively. Nucleotide sequencing was carried out in 46% of VP1/2A and in 53% of 5'NTR isolates. The identity in nucleotide sequence of the VP1/2A region ranged from 93.6 to 100.0%. Phylogenetic analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis A virus; Molecular epidemiology; Sub-genotype IA; Sub-genotype IB.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700004

Molecular epidemiology of type 1 and 2 dengue viruses in Brazil from 1988 to 2001
Provedor de dados: 56
Autores: Pires Neto,R.J.; Lima,D.M.; de Paula,S.O.; Lima,C.M.; Rocco,I.M.; Fonseca,B.A.L.
Dengue is a mosquito-borne viral infection that in recent decades has become a major international public health concern. Epidemic dengue fever reemerged in Brazil in 1981. Since 1990 more than one dengue virus serotype has been circulating in this tropical country and increasing rates of dengue hemorrhagic fever and dengue shock syndrome have been detected every year. Some evidence supports the association between the introduction of a new serotype and/or genotype in a region and the appearance of dengue hemorrhagic fever. In order to study the evolutionary relationships and possible detection of the introduction of new dengue virus genotypes in Brazil in the last years, we analyzed partial nucleotide sequences of 52 Brazilian samples of both dengue type...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dengue virus; Nucleotide sequence; E/NS1 region; Molecular epidemiology; Dengue fever; Dengue hemorrhagic fever.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600005

High incidence of oncogenic HPV genotypes found in women from Southern Brazil
Provedor de dados: 58
Autores: Entiauspe,Ludmila G; Silveira,Mariângela; Nunes,Emily M; Basgalupp,Suélen P; Stauffert,Dulce; Dellagostini,Odir A.; Collares,Tiago; Seixas,Fabiana K.
Oncogenic HPV genotypes are strongly associated with premalignant and malignant cervical lesion. The purpose was to determine human papillomavirus (HPV) prevalence and genotypes, and to estimate cervical cancer risk factor associations. Cervical samples were obtained from 251 women seeking gynecological care at the Pelotas School of Medicine Clinic. This is a cross-sectional study. HPV-DNA was amplified by nested-PCR using MY09/11 and GP5/6 primers, and the sequencing was used for genotyping. Sociodemographic and behavioral risk factors were obtained by closed questionnaire, and its relationship to HPV infection prevalence were analyzed. Statistical analyses were performed using SPSS 16.0 software, and differences were considered significant at p &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPV; SDT; Molecular epidemiology; Cervical cancer.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200043