Consulta: subjectFacets:"Molecular typing"
Registros recuperados: 11
Data/hora: 13/06/2024 00:34:00

Polymorphism of the rDNA and tDNA loci in clinical isolates of Pseudomonas aeruginosa: a perspective for molecular epidemiology surveillance
Provedor de dados: Genetics and Molecular Biology
Autores: Spacov,Isabel Cristina Guerra; Silva,Suzileyde Alberto Marques da; Morais Júnior,Marcos Antônio de; Morais,Márcia Maria Camargo de.
The Gram-negative bacterium Pseudomonas aeruginosa has a wide environmental and ecological distribution. It is an opportunistic pathogen that acquires resistance to multiple antimicrobial agents and can infect plants, animals and humans. We used rDNA and tDNA PCR markers to characterize the bacterial diversity of P. aeruginosa strains isolated at a Brazilian teaching hospital (Oswaldo Cruz University Hospital, Recife, Brazil) between March 2003 and February 2004. Clonal groups of P. aeruginosa clinical isolates were identified from different patients in different hospital units using either rDNA or tDNA markers, or a combination of both in a duplex PCR. These PCR-typing methods together with drug-resistance profiles were used to trace the distribution of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotic resistance; Molecular typing; Nosocomial infection; P. aeruginosa; RDNA-PCR; TDNA-PCR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400023

Arbitrarily primed PCR to type Vibrio spp. Pathogenic for shrimp
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Goarant, Cyrille; Merien, Fabrice; Berthe, Franck; Mermoud, Isabelle; Perolat, Philippe.
A molecular typing study on Vibrio strains implicated in shrimp disease outbreaks in New Caledonia and Japan was conducted by using AP-PCR (arbitrarily primed PCR). It allowed rapid identification of isolates at the genospecies level and studies of infraspecific population structures of epidemiological interest. Clusters identified within the species Vibrio penaeicida were related to their area of origin, allowing discrimination between Japanese and New Caledonian isolates, as well as between those from two different bays in New Caledonia separated by only 50 km. Other subclusters of New Caledonian V. penaeicida isolates could be identified, but it was not possible to link those differences to accurate epidemiological features. This contribution of AP-PCR...
Tipo: Text
Palavras-chave: Vibrio spp.; Shrimp; Molecular typing.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/publication-2734.pdf

Genotyping of Brazilian Giardia duodenalis human axenic isolates
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Coradi,ST; David,EB; Oliveira-Sequeira,TCG; Ribolla,PEM; Carvalho,TB; Guimarães,S.
Giardia duodenalis is a complex species that comprises at least seven distinct genetic groups (A to G), but only genotypes A and B are known to infect humans and a wide variety of other mammals. Regardless of biological, biochemical and antigenic analysis, several isolates maintained in vitro were not genetically typed yet. So, in the present study, five Brazilian axenic isolates obtained from asymptomatic and symptomatic patients were typed in order to determine the major genetic groups to which the isolates belonged. DNA was extracted from axenic trophozoites, fragments of glutamate dehydrogenase (gdh) and triosephosphate isomerase (tpi) genes were amplified by PCR and the isolate genotyping was carried out using restriction fragment length polymorphism...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Giardia duodenalis; Axenic isolates; Molecular typing; Genotype.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300016

Molecular characterization of Bacillus thuringiensis strains to control Spodoptera eridania (Cramer) (Lepidoptera: Noctuidae) population
Provedor de dados: Revista Brasileira de Entomologia
Autores: Machado,Déborah Heloísa Bittencourt; Livramento,Kalynka Gabriela do; Máximo,Wesley Pires Flausino; Negri,Bárbara França; Paiva,Luciano Vilela; Valicente,Fernando Hercos.
Abstract The main objective of this study was to characterize the toxicity and genetic divergence of 18 Bacillus thuringiensis strains in the biological control of Spodoptera eridania. Bacterial suspensions were added to the S. eridania diet. Half of the selected B. thuringiensis strains caused high mortality seven days after infection. The genetic divergence of B. thuringiensis strains was assessed based on Enterobacterial Repetitive Intergenic Consensus (ERIC) and Repetitive Extragenic Palindromic (REP) sequences, and five phylogenetic groups were formed. Despite their genetic diversity B. thuringiensis strains did not show any correlation between the collection sites and toxicity to larvae. Some B. thuringiensis strains are highly toxic to S. eridania,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Entomopathogenic bacteria; Insect pest; Molecular typing; Toxicity.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262020000100201

Molecular characteristics of methicillin-resistant Staphylococcus aureus isolates from hospital and community environments in northeastern Brazil
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Carvalho,Suzi P. de; Almeida,Jéssica B. de; Andrade,Yasmin M.F.S.; Silva,Lucas S.C. da; Chamon,Raiane C.; Santos,Kátia R.N. dos; Marques,Lucas M.
ABSTRACT This study characterized 30 MRSA isolates from intensive care unit (ICU) environment and equipment surfaces and healthy children. The SCCmec types I, IVa and V were detected in HA-MRSA isolates while CA-MRSA showed the SCCmec type IVa and V. Most isolates were classified as agr group II. All isolates presented the sei gene, and only HA-MRSA were positive for etb e tst genes. Three genotypes were related to Pediatric (ST5/SCCmecIV) and Berlin (ST45/SCCmecIV) clones. The present study showed molecular similarity between CA- and HA-MRSA isolates in hospital and community settings in a Brazilian region.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Methicillin-resistant Staphylococcus aureus; CA-MRSA; HA-MRSA; Molecular typing.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000200134

The use of molecular typing to evaluate the dissemination of antimicrobial resistance among gram-negative rods in Brazilian hospitals
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Tosin,Iraci; Silbert,Suzane; Sader,Helio S.
Antimicrobial resistance has increased rapidly in Brazil and worldwide during the past few years, giving rise to a growing necessity for antimicrobial resistance surveillance programs. These programs have been instituted in order to monitor bacterial resistance in various regions, and to guide empirical antimicrobial therapy. We evaluated the use of molecular typing in multicenter surveillance programs. We also studied the dissemination modes of selected resistance profiles. Antimicrobial susceptibility to various antimicrobial agents was evaluated by the reference broth microdilution method. Bacterial isolates with selected susceptibility patterns were characterized by pulsed field-gel electrophoresis (PFGE). A total of 119 Gram-negative bacteria were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular typing; Pulsed-field gel electrophoresis; Antimicrobial resistance; Carbapenem-resistant Pseudomonas aeruginosa; Clonal dissemination; Sentinela Program.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000600002

New multilocus sequence typing of MRSA in São Paulo, Brazil
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Carmo,M.S.; Inoue,F.; Andrade,S.S.; Paschoal,L.; Silva,F.M.; Oliveira,V.G.S.; Pignatari,A.C.C.
An increased incidence of nosocomial and community-acquired infections caused by methicillin-resistant Staphylococcus aureus (MRSA) has been observed worldwide. The molecular characterization of MRSA has played an important role in demonstrating the existence of internationally disseminated clones. The use of molecular biology methods in the surveillance programs has enabled the tracking of MRSA spread within and among hospitals. These data are useful to alert nosocomial infection control programs about the potential introduction of these epidemic clones in their areas. Four MRSA blood culture isolates from patients hospitalized at two hospitals in the city of São Paulo, Brazil, were analyzed; one of them was community acquired. The isolates were...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Methicillin-resistant Staphylococcus aureus; Molecular sequence typing; Resistance; Molecular typing.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000006

Typing of Enterococcus faecium by polymerase chain reaction and pulsed field gel electrophoresis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Bedendo,J.; Pignatari,A.C.C.
Polymerase chain reaction (PCR) with JB1 or REP consensus oligonucleotides and pulsed field gel electrophoresis (PFGE) were used to study genomic DNA extracted from 31 strains of enterococci. Eleven ATCC strains, representative of 11 species of Enterococcus, were initially tested by JB1-PCR, revealing that Enterococcus malodoratus and Enterococcus hirae presented identical banding patterns. Eight Enterococcus faecium isolates from Stanford University and 12 from São Paulo Hospital were studied by JB1-PCR, REP-PCR 1/2R and PFGE. Among the isolates from Stanford University, 5 genotypes were defined by JB1-PCR, 7 by REP-PCR 1/2R and 4 by PFGE. Among the isolates from São Paulo Hospital, 9 genotypes were identified by JB1-PCR, 6 by REP-PCR and 5 by PFGE. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular typing; Enterococcus faecium; PCR-PFGE.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100002

Extended-spectrum beta-lactamases in Klebsiella spp and Escherichia coli obtained in a Brazilian teaching hospital: detection, prevalence and molecular typing
Provedor de dados: Brazilian Journal of Microbiology
Autores: Freitas,Ana Lúcia Peixoto de; Machado,Denise Pires; Soares,Fabiana da Silva Correa; Barth,Afonso Luís.
His study was performed to compare the methods of detection and to estimate the prevalence of extended-spectrum beta-lactamases (ESBL) among Klebsiella spp and E.coli in a university hospital in southern Brazil. We also used a molecular typing method to evaluate the genetic correlation between isolates of ESBL K.pneumoniae. Production of ESBL was investigated in 95 clinical isolates of Klebsiella spp and Escherichia coli from Hospital de Clínicas de Porto Alegre, using Kirby-Bauer zone diameter (KB), double-disk diffusion (DD), breakpoint for ceftazidime (MIC CAZ), increased zone diameter with clavulanate (CAZ/CAC) and ratio of ceftazidime MIC/ceftazidime-clavulanate MIC (MIC CAZ/CAC). Molecular typing was performed by DNA macrorestriction analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ESBL; Klebsiella; Resistance; Molecular typing.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400012

LAMP technology: Rapid identification of Brucella and Mycobacterium avium subsp. paratuberculosis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Trangoni,Marcos D.; Gioffré,Andrea K.; Cerón Cucchi,María E.; Caimi,Karina C.; Ruybal,Paula; Zumárraga,Martín J.; Cravero,Silvio L.
In this study, we developed new sets of primers to detect Brucella spp. and M. avium subsp. paratuberculosis (MAP) through isothermal amplification. We selected a previously well-characterized target gene, bscp31, specific for Brucella spp. and IS900 for MAP. The limits of detection using the loop-mediated isothermal amplification (LAMP) protocols described herein were similar to those of conventional PCR targeting the same sequences. Hydroxynaphtol blue and SYBR GreenTM allowed direct naked-eye detection with identical sensitivity as agarose gel electrophoresis. We included the LAMP-based protocol in a rapid identification scheme of the respective pathogens, and all tested isolates were correctly identified within 2 to 3 h. In addition, both protocols...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Loop-mediated isothermal amplification; Molecular typing; Brucellosis; Paratuberculosis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200619

Molecular typing of Argentinian Mycobacterium avium subsp. paratuberculosis isolates by multiple-locus variable number-tandem repeat analysis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Gioffré,Andrea; Correa Muñoz,Magnolia; Alvarado Pinedo,María F.; Vaca,Roberto; Morsella,Claudia; Fiorentino,María Andrea; Paolicchi,Fernando; Ruybal,Paula; Zumárraga,Martín; Travería,Gabriel E.; Romano,María Isabel.
Multiple-locus variable number-tandem repeat analysis (MLVA) of Mycobacterium avium subspecies paratuberculosis (MAP) isolates may contribute to the knowledge of strain diversity in Argentina. Although the diversity of MAP has been previously investigated in Argentina using IS900-RFLP, a small number of isolates were employed, and a low discriminative power was reached. The aim of the present study was to test the genetic diversity among MAP isolates using an MLVA approach based on 8 repetitive loci. We studied 97 isolates from cattle, goat and sheep and could describe 7 different patterns: INMV1, INMV2, INMV11, INMV13, INMV16, INMV33 and one incomplete pattern. INMV1 and INMV2 were the most frequent patterns, grouping 76.3% of the isolates. We were also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paratuberculosis; Molecular typing; MIRU-VNTR; MLVA.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200557