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Characterisation and expression of four mRNA sequences encoding glutathione S-transferases pi, mu, omega and sigma classes in the Pacific oyster Crassostrea gigas exposed to hydrocarbons and pesticides
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Boutet, Isabelle; Tanguy, Aurelie; Moraga, Dario.
Hydrocarbon and pesticide pollution in coastal ecosystems can disturb marine bivalve metabolism. In this study, we characterised four full-length cDNA sequences encoding glutathione S-transferases (GSTs) in the Pacific oyster Crassostrea gigas. A BLAST X search showed that these four sequences encode GSTs from four different classes: GST pi, sigma, mu and omega. A phylogenetic analysis of GST was made to determine the position of oyster GST compared to invertebrate and vertebrate sequences. We developed a semi-quantitative, multiplex RT-PCR to follow the expression of these four GSTs in tissues of oysters exposed to hydrocarbons and two pesticide treatments ( glyphosate and a mixture composed of atrazine, diuron and isoproturon) under experimental...
Tipo: Text
Palavras-chave: Crassostrea gigas; Gene expression; Multiplex RTPCR; Pesticides; Hydrocarbons; CDNA sequences; Glutathione S transferases.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-431.pdf