Consulta: subjectFacets:"Multiplex-PCR"
Registros recuperados: 9
Data/hora: 16/06/2024 17:31:47

An analysis of multiplex-PCR in the detection of BCR-ABL transcripts in hematological disorders
Provedor de dados: 74
Autores: Sastre,Darío A.; Argaraña,Carlos E.; Heller,Viviana B.; Gallo,Mónica; Fernández,Enrique N.; Rodríguez,Cecilia M.
In this work, we describe the advantages of multiplex-PCR in the specific detection of BCR-ABL transcripts in different hematological disorders and its sensitivity compared to nested PCR. Fifty-three patients were studied for the presence of BCR-ABL transcripts: 24 patients with chronic myeloid leukemia (CML), 20 with acute leukemia (AL), and 9 patients with other hematological disorders. A variant rearrangement (b3a3) was found in a single case of CML (4.2%). Four out of the 20 patients with AL (20.0%) (14 adults, 6 children) were bcr-abl(+), and in this group three cases were classified as B-acute lymphoblastic leukemia (B-ALL), and one as acute myeloblastic leukemia (AML). Two of the three patients with B-ALL were positive for b2a2 and the other one for...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Multiplex-PCR; Ph chromosome; BCR-ABL; Chronic myeloid leukemia; PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400003

Neurological and epidemiological aspects of a BoHV-5 meningoencephalitis outbreak
Provedor de dados: 52
Autores: Lunardi,Michele; Claus,Marlise Pompeo; Lisbôa,Júlio Augusto Naylor; Amude,Alexandre Mendes; Headley,Selwyn Arlington; Alfieri,Alice Fernandes; Alfieri,Amauri Alcindo.
Bovine herpesvirus 5 is a DNA virus that has been associated with meningoencephalitis in young cattle. While its clinical diagnosis is obscured by other major diseases that also produce similar neurological disease in cattle, the use of conventional virological techniques is hampered by the establishment of a lifelong latent infection in the host and the difficulty in differentiating BoHV-1 and BoHV-5. The aim of the current report is to describe the clinical and epidemiological aspects observed in a natural outbreak of BoHV-5 meningoencephalitis in a dairy cattle herd from Brazil. In the outbreak, the affected animals consisted of nine calves, which presented three possible forms of the neurological disease, subjectively classified as peracute, acute, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Central nervous system; Bovine herpesvirus 5; Multiplex-PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700011

A search for Clostridium difficileribotypes 027 and 078 in Brazil
Provedor de dados: 55
Autores: Monteiro,Alexandre de Almeida; Pires Neto,Renata; Persson,Søren; Rodrigues Filho,Edison Moraes; Pasqualotto,Alessandro Comarú.
Toxigenic strains of Clostridium difficile may be disseminating. Here we prospectively screened patients with nosocomial diarrhoea in two hospitals in Brazil. To identify C. difficile polymerase chain reaction ribotypes 027/078 strains, we used high resolution melting and multiplex polymerase chain reaction. Among 116 screened patients, 11 were positive for C. difficile. The polymerase chain reaction ribotypes 027/078 strains were not identified in this study.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Multiplex-PCR; Clostridium difficile; HRM; Toxin gene profiling.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000600672

Comparison of multiplex-PCR and antigen detection for differential diagnosis of Entamoeba histolytica
Provedor de dados: 55
Autores: Santos,Helena Lúcia Carneiro; Peralta,Regina Helena Saramago; Macedo,Heloisa Werneck de; Barreto,Magali Gonçalves Muniz; Peralta,José Mauro.
Amebiasis is an infection caused by Entamoeba histolytica. However, differentiation between E. histolytica and Entamoeba dispar, which are morphologically identical species, is essential for treatment decision, precaution of the invasive disease and public health. The purpose of the present study was to evaluate a Multiplex -PCR for detection and differentiation of E. histolytica from E. dispar from fresh stool samples in comparison with the coproantigen commercial ELISA. Microscopic examination of stools using the Coprotest method, detection of stool antigen by enzyme-linked immunosorbent assay kit and a home made Multiplex-PCR, were used for the diagnosis of amoebiasis infection. Analysis of the 127 stools samples by microscopy examination demonstrated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multiplex-PCR; Entamoeba histolytica; Amebiasis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000300013

Bovine herpesvirus 5 detection by virus isolation in cell culture and multiplex-PCR in central nervous system from cattle with neurological disease in Brazilian herds
Provedor de dados: 58
Autores: Claus,Marlise Pompeo; Alfieri,Alice Fernandes; Médici,Kerlei Cristina; Lunardi,Michele; Alfieri,Amauri Alcindo.
Bovine herpesvirus 5 (BoHV-5) is an important cause of meningoencephalitis in young and adult cattle. The multiple etiology of neurological disturbances in cattle makes the quick and conclusive diagnosis of BoHV-5 infection important for animal and public health, mainly because of herbivore rabies that is endemic in Brazilian cattle herds. The objective of this retrospective study was to use a multiplex-polymerase chain reaction (multiplex-PCR) for BoHV-5 and BoHV-1 glycoprotein C gene detection from stored central nervous system (CNS) tissue fragments of cattle with neurological clinical signs. Forty-seven frozen CNS samples of young and adult cattle from 31 herds in three Brazilian geographical regions (South, Southeast, and Center-west) were evaluated....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Meningoencephalitis; Bovine herpesvirus 5; Multiplex-PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300019

Identification of Mycobacterium bovis Isolates by a multiplex PCR
Provedor de dados: 58
Autores: Figueiredo,Eduardo Eustáquio de Souza; Silvestre,Flávia Galindo; Campos,Wilma Neres; Furlanetto,Leone Vinícius; Medeiros,Luciana; Lilenbaum,Walter; Fonseca,Leila Sousa; Silva,Joab Trajano; Paschoalin,Vânia Margaret Flosi.
Isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-PCR) targeting for RvD1Rv2031c and IS6110 sequences, specific for M. bovis and Mycobacterium tuberculosis complex respectively. The m-PCR successfully identified as M. bovis 88.24% of the isolates.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium bovis; Multiplex-PCR; Bovine tuberculosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200004

Virulence genes and antimicrobial resistance of Pasteurella multocida isolated from poultry and swine
Provedor de dados: 58
Autores: Furian,Thales Quedi; Borges,Karen Apellanis; Laviniki,Vanessa; Rocha,Silvio Luis da Silveira; Almeida,Camila Neves de; Nascimento,Vladimir Pinheiro do; Salle,Carlos Tadeu Pippi; Moraes,Hamilton Luiz de Souza.
Abstract Pasteurella multocida causes atrophic rhinitis in swine and fowl cholera in birds, and is a secondary agent in respiratory syndromes. Pathogenesis and virulence factors involved are still poorly understood. The aim of this study was to detect 22 virulence-associated genes by PCR, including capsular serogroups A, B and D genes and to evaluate the antimicrobial susceptibility of P. multocida strains from poultry and swine. ompH, oma87, plpB, psl, exbD-tonB, fur, hgbA, nanB, sodA, sodC, ptfA were detected in more than 90% of the strains of both hosts. 91% and 92% of avian and swine strains, respectively, were classified in serogroup A. toxA and hsf-1 showed a significant association to serogroup D; pmHAS and pfhA to serogroup A. Gentamicin and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pasteurellosis; Virulence factors; Multiplex-PCR; Antimicrobial susceptibility.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100210

Detection of CDT toxin genes in Campylobacter spp. strains isolated from broiler carcasses and vegetables in São Paulo, Brazil
Provedor de dados: 58
Autores: Carvalho,Aline Feola de; Silva,Daniela Martins da; Azevedo,Sergio Santos; Piatti,Rosa Maria; Genovez,Margareth Elide; Scarcelli,Eliana.
Campylobacteriosis is a worldwide distributed zoonosis. One of the main virulence factors related to Campylobacter spp. in animals and humans is the cytolethal distending toxin (CDT), encoded by three adjacent genes (cdtA, cdtB, cdtC). The occurrence of Campylobacter spp. in samples of vegetables has not been reported in Brazil yet, and has seldom been described in the international literature. The detection of CDT in these strains has not been reported, either. The objectives of the present study were to determine the occurrence of Campylobacter spp. strains carrying virulence factors in samples of poultry and vegetables (lettuce and spinach) from different points of sale, thus verifying if vegetables are as an important vehicle for potentially virulent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Campylobacter spp.; Cytolethal distending toxin (CDT); Broiler carcasses; Vegetables; Multiplex-PCR.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300005

Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
Provedor de dados: 58
Autores: Figueiredo,Eduardo Eustáquio de Souza; Carvalho,Ricardo Cezar Tavares; Silvestre,Flávia Galindo; Lilenbaum,Walter; Fonseca,Leila Sousa; Silva,Joab Trajano; Paschoalin,Vânia Margaret Flosi.
Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9%). The use of mPCR of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium bovis; Multiplex-PCR; Nasal swab; Bovine tuberculosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200020