Consulta: subjectFacets:"Mycoplasma"
Registros recuperados: 31
Data/hora: 01/06/2024 18:27:06

ABC transporters in Mycoplasma hyopneumoniae and Mycoplasma synoviae: insights into evolution and pathogenicity
Provedor de dados: Genetics and Molecular Biology
Autores: Nicolás,Marisa Fabiana; Barcellos,Fernando Gomes; Nehab Hess,Pablo; Hungria,Mariangela.
ABC transporters represent one of the largest superfamilies of active membrane transport proteins (MTPs) with a highly conserved ATPase domain that binds and hydrolyzes ATP, supplying energy for the uptake of a variety of nutrients and for the extrusion of drugs and metabolic wastes. The complete genomes of a non-pathogenic (J) and pathogenic (7448) strain of Mycoplasma hyopneumoniae, as well as of a pathogenic (53) strain of Mycoplasma synoviae have been recently sequenced. A detailed study revealed a high percentage of CDSs encoding MTPs in M. hyopneumoniae strains J (13.4%), 7448 (13.8%), and in M. synoviae 53 (11.2%), and the ABC systems represented from 85.0 to 88.6% of those CDSs. Uptake systems are mainly involved in cell nutrition and some might be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ABC transporters; Bioinformatics; Genomes of microorganisms; Membrane transport proteins; Mycoplasma; Phylogeny of prokaryotes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200006

Insertion sequences as variability generators in the Mycoplasma hyopneumoniae and M. synoviae genomes
Provedor de dados: Genetics and Molecular Biology
Autores: Loreto,Elgion Lúcio Silva; Ortiz,Mauro Freitas; Porto,Jorge Ivan Rebelo.
We have analyzed the sequenced genomes of three strains of Mycoplasma hyopneumoniae and one strain of M. synoviae, and have found three and two different transposable element families, respectively in each species. In M. hyopneumoniae, the Insertion Sequences of the IS4 family is represented by ISMHp1, a putatively active element. The IS3 family is represented by several degenerated sequences. A third element called tMH was found, which shows some characteristics reminiscent of retrotransposons. In M. synoviae, three different possibly active IS4 elements are present (ISMHp1-like; ISMs1 and IS1634-like elements). The IS30 family is represented by the degenerated IS1630-like element. The IS1634-like element is shown to be involved in chromosomal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insertion sequences; IS; Mollicutes; Horizontal gene transfer; Mycoplasma.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200017

Kinases of two strains of Mycoplasma hyopneumoniae and a strain of Mycoplasma synoviae: an overview
Provedor de dados: Genetics and Molecular Biology
Autores: Bailão,Alexandre Melo; Parente,Juliana Alves; Pereira,Maristela; Soares,Célia Maria de Almeida.
Mycoplasma synoviae and Mycoplasma hyopneumoniae are wall-less eubacteria belonging to the class of Mollicutes. These prokaryotes have a reduced genome size and reduced biosynthetic machinery. They cause great losses in animal production. M. synoviae is responsible for an upper respiratory tract disease of chickens and turkeys. M. hyopneumoniae is the causative agent of enzootic pneumonia in pigs. The complete genomes of these organisms showed 17 ORFs encoding kinases in M. synoviae and 15 in each of the M. hyopneumoniae strain. Four kinase genes were restricted to the avian pathogen while three were specific to the pig pathogen when compared to each other. All deduced kinases found in the non pathogenic strain (J[ATCC25934]) were also found in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma; Kinases; Genomes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200008

Comparative genome analysis of proteases, oligopeptide uptake and secretion systems in Mycoplasma spp
Provedor de dados: Genetics and Molecular Biology
Autores: Staats,Charley Christian; Boldo,Juliano; Broetto,Leonardo; Vainstein,Marilene; Schrank,Augusto.
Mycoplasmas are very fastidious in their nutritional requirements for in vitro growth and have limited biosynthetic capacity, a reflection of their reduced genomes. As a result, these bacteria depend upon external metabolites for nutrition and growth and have developed dependence on their hosts for survival and maintenance. Protein degradation and peptide importation play an important role in Mycoplasma spp. nutrition, and proteases can play a role in host adaptation and pathogenicity. Here, we present a general survey on the genes involved in protein degradation, secretion and importation, comparing all available Mollicute genomes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma; Proteases; Minimal genomes; Secretion systems.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200009

Molecular analysis of an integrative conjugative element, ICEH, present in the chromosome of different strains of Mycoplasma hyopneumoniae
Provedor de dados: Genetics and Molecular Biology
Autores: Pinto,Paulo Marcos; Carvalho,Marcos Oliveira de; Alves-Junior,Leonardo; Brocchi,Marcelo; Schrank,Irene Silveira.
Diversification of bacterial species and pathotypes is largely caused by lateral gene transfer (LGT) of diverse mobile DNA elements such as plasmids, phages, transposons and genomic islands. Thus, acquisition of new phenotypes by LGT is very important for bacterial evolution and relationship with hosts. This paper reports a 23 kb region containing fourteen CDSs with similarity to the previous described Integrative Conjugal Element of Mycoplasma fermentans (ICEF). This element, named ICEH, is present as one copy at distinct integration sites in the chromosome of 7448 and 232 pathogenic strains and is absent in the type strain J (non-pathogenic). Notable differences in the nucleotide composition of the insertion sites were detected, and could be correlated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: LGT; Mycoplasma; Constins.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200014

A phylogenomic appraisal of the evolutionary relationship of mycoplasmas
Provedor de dados: Genetics and Molecular Biology
Autores: Yotoko,Karla S.C.; Bonatto,Sandro L.
Several genomes of mycoplasmas have been sequenced and here we tried to retrieve the evolutionary relationships of nine species using a phylogenomic approach. Several methods were used to build phylogenetic trees based on protein sequence information, gene-order, and gene-content. We also utilized datasets composed of individual and concatenated sets of orthologous proteins, as well as with reduced unreliable alignment regions. Most of our results converge to a single topology, except for the trees built with both the maximum parsimony method and with the gene-order dataset. The gene-content dataset presented trees consistent with most nodes of the convergent tree, but in the gene-order dataset most internal branches were clearly saturated and unreliable....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phylogenomic; Mycoplasma; Phylogenetic methods; Maximum likelihood; Maximum parsimony.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200015

Identification of the GTPase superfamily in Mycoplasma synoviae and Mycoplasma hyopneumoniae
Provedor de dados: Genetics and Molecular Biology
Autores: Borges,Clayton Luiz; Parente,Juliana Alves; Pereira,Maristela; Soares,Célia Maria de Almeida.
Mycoplasmas are the smallest known prokaryotes with self-replication ability. They are obligate parasites, taking up many molecules of their hosts and acting as pathogens in men, animals, birds and plants. Mycoplasma hyopneumoniae is the infective agent of swine mycoplasmosis and Mycoplasma synoviae is responsible for subclinical upper respiratory infections that may result in airsacculitis and synovitis in chickens and turkeys. These highly infectious organisms present a worldwide distribution and are responsible for major economic problems. Proteins of the GTPase superfamily occur in all domains of life, regulating functions such as protein synthesis, cell cycle and differentiation. Despite their functional diversity, all GTPases are believed to have...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma; GTPase superfamily; Genome.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200007

Differential metabolism of Mycoplasma species as revealed by their genomes
Provedor de dados: Genetics and Molecular Biology
Autores: Arraes,Fabricio B.M.; Carvalho,Maria José A. de; Maranhão,Andrea Q.; Brígido,Marcelo M.; Pedrosa,Fábio O.; Felipe,Maria Sueli S.
The annotation and comparative analyses of the genomes of Mycoplasma synoviae and Mycoplasma hyopneumonie, as well as of other Mollicutes (a group of bacteria devoid of a rigid cell wall), has set the grounds for a global understanding of their metabolism and infection mechanisms. According to the annotation data, M. synoviae and M. hyopneumoniae are able to perform glycolytic metabolism, but do not possess the enzymatic machinery for citrate and glyoxylate cycles, gluconeogenesis and the pentose phosphate pathway. Both can synthesize ATP by lactic fermentation, but only M. synoviae can convert acetaldehyde to acetate. Also, our genome analysis revealed that M. synoviae and M. hyopneumoniae are not expected to synthesize polysaccharides, but they can take...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mollicutes; Mycoplasma; Metabolism; Comparative genomics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200004

Cosmopolitan Distribution of Endozoicomonas-Like Organisms and Other Intracellular Microcolonies of Bacteria Causing Infection in Marine Mollusks
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Cano, Irene; Ryder, David; Webb, Steve C.; Jones, Brian J.; Brosnahan, Cara L.; Carrasco, Noelia; Bodinier, Barbara; Furones, Dolors; Pretto, Tobia; Carella, Francesca; Chollet, Bruno; Arzul, Isabelle; Cheslett, Deborah; Collins, Evelyn; Lohrmann, Karin B.; Valdivia, Ana L.; Ward, Georgia; Carballal, María J.; Villalba, Antonio; Marigómez, Ionan; Mortensen, Stein; Christison, Kevin; Kevin, Wakeman C.; Bustos, Eduardo; Christie, Lyndsay; Green, Matthew; Feist, Stephen W.
Intracellular microcolonies of bacteria (IMC), in some cases developing large extracellular cysts (bacterial aggregates), infecting primarily gill and digestive gland, have been historically reported in a wide diversity of economically important mollusk species worldwide, sometimes associated with severe lesions and mass mortality events. As an effort to characterize those organisms, traditionally named as Rickettsia or Chlamydia-like organisms, 1950 specimens comprising 22 mollusk species were collected over 10 countries and after histology examination, a selection of 99 samples involving 20 species were subjected to 16S rRNA gene amplicon sequencing. Phylogenetic analysis showed Endozoicomonadaceae sequences in all the mollusk species analyzed....
Tipo: Text
Palavras-chave: Mollusk; OTU; Endozoicomonas; Chlamydiae; Mycoplasma; Francisella; Lucinoma; Ridgeia.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00658/77032/78322.pdf

Disturbance induced decoupling between host genetics and composition of the associated microbiome
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Wegner, Karl Mathias; Volkenborn, Nils; Peter, Hannes; Eiler, Alexander.
Background  Studies of oyster microbiomes have revealed that a limited number of microbes, including pathogens, can dominate microbial communities in host tissues such as gills and gut. Much of the bacterial diversity however remains underexplored and unexplained, although environmental conditions and host genetics have been implicated. We used 454 next generation 16S rRNA amplicon sequencing of individually tagged PCR reactions to explore the diversity of bacterial communities in gill tissue of the invasive Pacific oyster Crassostrea gigas stemming from genetically differentiated beds under ambient outdoor conditions and after a multifaceted disturbance treatment imposing stress on the host. Results  While the gill associated microbial communities in...
Tipo: Text
Palavras-chave: Microbiota; Population structure; Stress; Pathogen; Biological invasion; Pacific oyster; Crassostrea gigas; Vibrio; Mycoplasma.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00171/28202/26430.pdf

Handbook of pigeonpea diseases
Provedor de dados: Open Acess Agriculture Research Repository
Autores: M.V.
Palavras-chave: Pigeonpeas; Fungi; Phoma; Mycoplasma; Soil water regimes; Ratoons; Diseases; Sole cropping; Seed dressing; Soil solarization.
Ano: 1993
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4234

Field diagnosis of groundnut diseases
Provedor de dados: Open Acess Agriculture Research Repository
Autores: P.
Palavras-chave: Fungi; Groundnuts; Necrosis; Fruits; Diseases; Mycoplasma; Viruses; Bacteria; Environmental factors.
Ano: 1992
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4249

Field diagnosis of chickpea diseases and their control
Provedor de dados: Open Acess Agriculture Research Repository
Autores: Y.L.
Palavras-chave: Chickpeas; Fungi; Phoma; Vegetative stage; Mycoplasma; Necrosis; Diseases; Sowing date; Soil water content; Fruits.
Ano: 1991
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4248

Economic Consequences of Biological Variation: The Case of Mycoplasma in Swine Herds
Provedor de dados: Research in Agricultural & Applied Economics (AgEcon Searh)
Autores: Otto,   Lars.
We present an economic decision support model, based on a Bayesian network, for Mycoplasma infection in slaughter swine production. The model describes the various risk factors for Mycoplasma infection and their interactions. This leads to a stochastic determination of the consequences of productivity factors, and this again results in stochastic economic consequences when changing the risk factors or engaging in control arrangements. We use the model to calculate how estimated prevalence and level of Mycoplasma changes when we gather evidence from various veterinarian examinations, and we show how this influences the distribution of the economic results of a change in strategy to fight Mycoplasma. One result is that the profitability in fighting...
Tipo: Conference Paper or Presentation
Palavras-chave: Decision support system; Bayesian network; Pig; Mycoplasma; Livestock Production/Industries; C11; D81; Q12.
Ano: 2005
URL: http://purl.umn.edu/24458

Circulation of Major Respiratory Pathogens in Backyard Poultry and their Association with Clinical Disease and Biosecurity
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Batista,IA; Hoepers,PG; Silva,MFB; Nunes,PLF; Diniz,DCA; Freitas,AG; Cossi,MVC; Fonseca,BB.
SUMMARY Raising backyard birds is a common practice in Brazil, mainly in the countryside or suburban areas. However, the level of respiratory pathogens in these animals is unknown. We sampled two hundred chickens from 19 backyard flocks near commercial poultry farms and performed ELISA to Infectious Bronchitis Virus, avian Metapneumovirus, Mycoplasma synoviae and Mycoplasma gallisepticum. We evaluated the association between the predictive ability of ELISA and Hemagglutination-inhibition (HI)by comparing results from eight flocks positive to Mycoplasma gallisepticum on ELISA. Besides, we assessed essential biosecurity measures in the properties (multiple species birds, rodent control, hygienic conditions, and water quality for the bird`s consumption). We...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avian Metapneumovirus; ELISA; Hemagglutination-inhibition; Infectious Bronchitis Virus; Mycoplasma.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100324

Serologic response of SPF chickens to live vaccines and other strains of Mycoplasma gallisepticum
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Nascimento,ER do; Polo,P de A; Pereira,VL de A; Barreto,ML; Nascimento,M da GF do; Zuanaze,MAF; Corrêa,ARA; Silva,R de CF.
False positive serologic reactions and difficulties in the diagnosis of Mycoplasma gallisepticum (MG) in chickens have increased lately as a result of infection by low virulent MG strains and the use of live MG vaccines in poultry. The objective of this study was to evaluate the serologic responses of SPF chickens exposed to the three commercially available live MG vaccines, and one low virulent MG strain (MG-70), contributing to the diagnosis and monitoring of MG infection in birds. Six groups of SPF chickens were used. The control group was not infected nor challenged; one group was infected with the low virulent strain MG-70 (MG-70); three groups were immunized and named after the MG vaccine used, i.e., MG-6/85, MG-ts11, and MG-F; and finally one group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma; Poultry; Serology; Vaccination.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000100007

Avian mycoplasmosis update
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Nascimento,ER; Pereira,VLA; Nascimento,MGF; Barreto,ML.
Avian mycoplasmas occur in a variety of bird species. The most important mycoplasmas for chickens and turkeys are Mycoplasma gallisepticum (MG), M. synoviae (MS), and M. meleagridis. Besides, M. iowe (MI) is an emerging pathogen in turkeys, but of little concern for chickens. Mycoplasmas are bacteria that lack cell wall and belong to the class Mollicutes. Although they have been considered extracellular agents, scientists admit nowadays that some of them are obligatory intracellular microorganisms, whereas all other mycoplasmas are considered facultative intracellular organisms. Their pathogenic mechanism for disease include adherence to host target cells, mediation of apoptosis, innocent bystander damage to host cell due to intimate membrane contact,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Airsacculitis; Diagnosis; Mycoplasma; Mollicutes; Poultry.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2005000100001

Hemoparasites and hemotropic bacteria detected through direct microscopy in dogs peripheric blood samples in a veterinarian private clinic in the municipality of Lorena, São Paulo, Brazil
Provedor de dados: Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP
Autores: Gavião Prado, Luan; Vieira Sene, Rafael; Camargo de Medeiros, Elisângela; Maioli do Nascimento, Letícia; Beatriz França de Faria, Ana.
Hemoparasitosis, diseases that may cause clinical and hematological complications, are considered a great challenge to practitioners and clinical pathologists. Its high incidence in all Brazilian territory and the difficulty for a precise diagnosis can lead in most cases to a therapeutic diagnosis. Anaplasma platys, Babesia canis vogeli, Ehrlichia canis, Mycoplasma haemocanis and with a lower frequency and clinical relevance, Anaplasma phagocytophilum are the main etiological agents affecting dogs in Brazil. The climatic conditions found in several Brazilian areas, such as high rain and temperature levels, favor the arthropods vectors life cycles. This subject became an important challenge to both veterinarians and pet owners. These etiological agents...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemoparasite; Anaplasma; Babesia; Mycoplasma; Ehrlichia; Hemoparasitose; Anaplasma; Babesia; Mycoplasma; Ehrlichia.
Ano: 2019
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/37999

Plasmids in Mycoplasma species isolated from goats and sheep and their preliminary typing
Provedor de dados: Revista de Microbiologia
Autores: Nascimento,Elmiro R.; DaMassa,Al J.; Yamamoto,Richard; Nascimento,M. Graça F.
One-hundred-five (105) clinical isolates of mycoplasma from caprine origin and one isolate from ovine were surveyed for plasmids, which were present in thirty-three (31%) of them. These mycoplasmas originated from 13 herds. Ten of them were symptomatic for mycoplasmal disease (mastitis, polyarthritis, septicemia) and three herds were asymptomatic, i.e., clinically normal. Twenty-eight isolates were Mycoplasma mycoides subspecies mycoides LC (large colony or caprine biotype), four were Mycoplasma capricolum subsp. capricolum and one was Mycoplasma cottewii. The isolated plasmids were linearized by EcoRI, EcoRV, EcoRI and EcoRV or BamHI and EcoRV, and were of five sizes (1.1, 1.6, 1.7, 1.8, and 1.9 Kbp). Based on restriction enzyme digestion and size of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma; Plasmid; Goat; Endonuclease; Electrophoresis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100006

Molecular biology of mycoplasmas: from the minimum cell concept to the artificial cell
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: CORDOVA,CAIO M.M.; HOELTGEBAUM,DANIELA L.; MACHADO,LAÍS D.P.N.; SANTOS,LARISSA DOS.
ABSTRACT Mycoplasmas are a large group of bacteria, sorted into different genera in the Mollicutes class, whose main characteristic in common, besides the small genome, is the absence of cell wall. They are considered cellular and molecular biology study models. We present an updated review of the molecular biology of these model microorganisms and the development of replicative vectors for the transformation of mycoplasmas. Synthetic biology studies inspired by these pioneering works became possible and won the attention of the mainstream media. For the first time, an artificial genome was synthesized (a minimal genome produced from consensus sequences obtained from mycoplasmas). For the first time, a functional artificial cell has been constructed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma; Molecular biology; Microbial genetics; Synthetic biology; Gene transfer techniques.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200599

Intra-uterine experimental infection by Ureaplasma diversum induces TNF-α mediated womb inflammation in mice
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: Silva,Jamile R.; Ferreira,Lício F.A.A.; Oliveira,Percíllia V.S.; Nunes,Ivanéia V.; Pereira,Ítalo S.; Timenetsky,Jorge; Marques,Lucas M.; Figueiredo,Tiana B.; Silva,Robson A.A.
Ureaplasma diversum is an opportunistic pathogen associated with uterine inflammation, impaired embryo implantation, infertility, abortions, premature birth of calves and neonatal pneumonia in cattle. It has been suggested that the intra-uterine infection by Ureaplasma diversum can cause vascular changes that hinder the success of pregnancy. Thus, the aim of this study was to evaluate the changes of intrauterine site of A/J mice in estrus or proestrus phase inoculated with Ureaplasma diversum. The infection was monitored at 24, 48 and 72 hours by the PCR methodology to detect the Ureaplasma in the inoculation site and the profile of circulating blood cells. Morphological changes, intensity of inflammation and the production of cytokines were compared. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma; Uterus; Ureaplasma; Mice.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200643

Mycoplasma hyorhinis and Mycoplasma fermentans induce cell apoptosis and changes in gene expression profiles of 32D cells
Provedor de dados: Biological Research
Autores: Liu,Wenbin; Shou,Chengchao.
Infection of mycoplasmas has been linked to various human diseases including arthritis, pneumonia, infertility and cancer. While Mycoplasma hyorhinis and Mycoplasma fermentans have been detected in gastric adenocarcinomas, the mechanisms underlyine the pathogenesis are unknown. In this study, cell growth kinetics, Hoechst 33258 staining, DNA ladder assays, Western blotting analysis and cDNA microarray assays were performed to investigate the roles of M. hyorhinis and M. fermentans during infection of mammalian cells. Our data demonstrated that these mycoplasmas inhibid the growth of immortalised cell lines (32D and COS-7) ane tumor cell lines (HeLa and AGS). In addition, the infection of the 32D cell line with M. hyorhinis and M. fermentans induced...
Tipo: Journal article
Palavras-chave: Mycoplasma; Apoptosis; Gene expression profiles.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400011

Quality Control of Biotechnological Inputs DetectingMycoplasma
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Netto,Cristiane; Soccol,Vanete Thomaz; Sepulveda,Lya; Garcia,Gabriel Henrique Oliveira; Timenetsky,Jorge.
The aim of this work was to study the Polymerase Chain Reaction (PCR) as a tool of quality control of bovine sera and cellular cultures used in the biotechnological industry. A total of 46 samples of bovine sera derived from two slaughterhouses and 33 samples of BHK21 cells derived from two biotechnological industries were evaluated using the primers GPO-3 (sense) and MGSO (antisense). The PCR technique sensibility analysis showed that 280 bp were amplified for the quantities of 50 ng to 0.006 ng of Micoplasma DNA. The primers specificity was confirmed in the test using Staphylococcus aureus, Escherichia coli, Bacillus subtilisand Candida albicans; except by the positive control, none of the samples showed amplification. The presence of Mycoplasma in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Bovine serum; BHK21 cells; Mycoplasma; Cellular culture.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200239

Mycoplasmateceae species are not found in Fallopian tubes of women with tubo-peritoneal infertility
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Costoya,Alberto; Morales,Francisco; Borda,Paula; Vargas,Renato; Fuhrer,Juan; Salgado,Nicole; Cárdenas,Hugo; Velasquez,Luis.
BACKGROUND: The role of mycoplasmas on the development and sequelae of pelvic inflammatory disease remains controversial. The objective of the present study is to correlate directly the presence of Mycoplasmateceae through polimerase chain reaction (PCR) determinations in cervix and Fallopian tubes of infertile patients with tubo-peritoneal factor diagnosed through laparoscopy. METHODS: Thirty patients with tubo-peritoneal infertility and 30 normal fertile patients were included in the study; cervical samples and tubal flushings were obtained during laparoscopy. PCR determinations for the detection of genetic material of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealiticum, Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma; Mycoplasmateceae; Fallopian tubes; Tuboperitoneal infertility; Infections transmitted sexually.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000300010

Thioglycollate-elicited murine macrophages are cytotoxic to Mycoplasma arginini-infected YAC-1 tumor cells
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ribeiro-Dias,F.; Russo,M.; Nascimento,F.R.F.; Barbuto,J.A.M.; Timenetsky,J.; Jancar,S.
Macrophages are important components of natural immunity involved in inhibition of tumor growth and destruction of tumor cells. It is known that these cells can be activated for tumoricidal activity by lymphokines and bacterial products. We investigated whether YAC-1 tumor cells infected with Mycoplasma arginini stimulate nitric oxide (NO) release and macrophage cytotoxic activity. Thioglycollate-elicited macrophages from male BALB/c mice were co-cultured for 20 h with YAC-1 tumor cells infected or not with Mycoplasma arginini. The cytotoxic activity was evaluated by MTT assay and nitrite levels were determined with the Griess reagent. Thioglycollate-elicited macrophages co-cultured with noninfected YAC-1 cells showed low cytotoxic activity (34.7 ± 8.6%)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Macrophages; Cytotoxicity; Mycoplasma; Tumor cells; Nitric oxide.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100010

Clastogenic effects of different Ureaplasma urealyticum serovars on human chromosomes
Provedor de dados: Brazilian Journal of Medical and Biological Research
The possibility that Ureaplasma urealyticum might play an important role in human infertility was first raised more than 20 years ago, but this association remains speculative. Considering the hypothesis that the pathogenicity of Ureaplasma urealyticum may depend on its serotypes, the clastogenic effects of different strains of Ureaplasma urealyticum, at concentrations of 103 CCU (color changing units)/ml, 104 CCU/ml and 105 CCU/ml, were evaluated in vitro in short-term cultures of human lymphocytes. Total or partial mitotic inhibition was produced by Ureaplasma urealyticum serotypes 2, 3 and 10 independent of the concentration (103 CCU/ml, 104 CCU/ml or 105 CCU/ml) of the microorganisms employed. In contrast, the clastogenic effects observed with...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mycoplasma; Chromosome alterations; Ureaplasma urealyticum; Clastogenic effects.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600008

Description of Lyme disease-like syndrome in Brazil: is it a new tick borne disease or Lyme disease variation?
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Mantovani,E.; Costa,I.P.; Gauditano,G; Bonoldi,V.L.N.; Higuchi,M.L.; Yoshinari,N.H.
An emerging clinical entity that reproduces clinical manifestations similar to those observed in Lyme disease (LD) has been recently under discussion in Brazil. Due to etiological and laboratory particularities it is named LD-like syndrome or LD imitator syndrome. The condition is considered to be a zoonosis transmitted by ticks of the genus Amblyomma, possibly caused by interaction of multiple fastidious microorganisms originating a protean clinical picture, including neurological, osteoarticular and erythema migrans-like lesions. When peripheral blood of patients with LD-like syndrome is viewed under a dark-field microscope, mobile uncultivable spirochete-like bacteria are observed. PCR carried out with specific or conservative primers to recognize...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lyme disease-like syndrome; Tick-borne disease; Mycoplasma; Chlamydia; Spirochete; Lyme disease.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400002

Detection of multiple mycoplasma infection in cell cultures by PCR
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Timenetsky,J.; Santos,L.M.; Buzinhani,M.; Mettifogo,E.
A total of 301 cell cultures from 15 laboratories were monitored for mycoplasma (Mollicutes) using PCR and culture methodology. The infection was detected in the cell culture collection of 12 laboratories. PCR for Mollicutes detected these bacteria in 93 (30.9%) samples. Although the infection was confirmed by culture for 69 (22.9%) samples, PCR with generic primers did not detect the infection in five (5.4%). Mycoplasma species were identified with specific primers in 91 (30.2%) of the 98 samples (32.6%) considered to be infected. Mycoplasma hyorhinis was detected in 63.3% of the infected samples, M. arginini in 59.2%, Acholeplasma laidlawii in 20.4%, M. fermentans in 14.3%, M. orale in 11.2%, and M. salivarium in 8.2%. Sixty (61.2%) samples were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma; Cell culture; Multiple infection; Mycoplasma detection.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700009

Mycoplasma synoviae infection on Newcastle disease vaccination of chickens
Provedor de dados: Brazilian Journal of Microbiology
Autores: Silva,Rita de Cássia Figueira; Nascimento,Elmiro Rosendo do; Pereira,Virgínia Léo de Almeida; Barreto,Maria Lúcia; Nascimento,Maria da Graça Fichel do.
Newcastle disease is characterized by respiratory manifestations in association with nervous and/or digestive symptoms. Its prevention is done by vaccination with live attenuated (lentogenic strains) and/or killed vaccines. The lentogenic strains can lead to strong post-vaccination reaction, principally due to the presence of other pathogenic agents. Among them, Mycoplasma synoviae is worldwide important, mainly in Brazil. The dissemination of this agent in poultry flocks has been achieved due to difficulties in diagnosis and disease reproduction, virulence variations among different M.synoviae strains, and attribution of typical M.synoviae disease manifestation to other disease agents. This experimental study in SPF chicks (Gallus gallus), previously...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vaccine; Serology; Mycoplasma; Newcastle disease.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200033

Sensitivity of rabbit fibrochondrocytes to mycoplasmas
Provedor de dados: Brazilian Journal of Microbiology
Autores: Nascimento,Carlos Manuel de Oliveira; Figueiredo,Cristina Adelaide; Timenetsky,Jorge.
Primary cell culture from rabbit meniscus (fibrochondrocytes-FcrC) was infected for 24 hours with different inocula (10² to 10(7) Colony Forming Units-CFU) of Mycoplasma hominis PG-21, M. pneumoniae FH and 1428 or M. arthritidis PG-6. The severity of the different obtained cytophatic effects-CPE was inoculum, Mycoplasma species and strain dependant. These bacteria were recovered from all infected FcrC and the SP4 medium for mycoplasmas also caused toxic effect on the FcrC. It was concluded that rabbit fibrochondrocytes were sensitive to mycoplasma infection, as well as to the SP4 mycoplasma medium.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma; Fibrochondrocytes; Arthritis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300011

Mycoplasmas hyorhinis in different regions of cuba: diagnosis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Lobo,Evelyn; Poveda,Carlos; Suarez,Alejandro; Hernández,Yenney; Ramírez,Ana; Poveda,José B.
M. hyorhinis is considered one of the etiological agents of arthritis in sucking pigs, but recently as seen, some strains can produce pneumonia that could not be distinguished from the mycoplasmosis caused by M. hyopneumoniae. The study was conducted to research the presence of Mycoplasma hyorhinis (M. hyorhinis ) in different regions of the country from exudates of pig lungs with typical EP lesions. Exudates from 280 pig lungs with typical EP lesions were studied using molecular techniques such as PCR, real time PCR and amplification of the 16S-23S rRNA. It was detected that the 66% of the samples studied resulted positive to M. hyorhinis, and the presence of this species was detected in all the provinces. Amplification and studies on the intergenic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Mycoplasma; Diagnostic; Enzootic pneumonia.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200039