Consulta: subjectFacets:"Myotoxicity"
Registros recuperados: 9
Data/hora: 16/06/2024 14:55:16

AN IN VITRO STUDY OF THE EFFECTS OF VENOM OF AUSTRALIAN ELAPIDS ON MURINE SKELETAL MUSCLE AND THE PROTECTIVE EFFECT OF HOMOLOGOUS PLASMA
Provedor de dados: Journal of Venomous Animals and Toxins
Autores: BUTLER,T. L.; JACOBSEN,P. F.; MIRTSCHIN,P. J.; KAKULAS,B. A.
The venom of many dangerous Australian snakes has a myotoxic component and some are strongly myolytic. The myotoxicity of venom of seven Australian elapid snakes was studied to determine their relative in vitro potency in causing cell death of C2C12 cells, a myoblast cell line, and murine myotubes in mixed cell culture. The venom of Pseudechis australis proved to be the most myotoxic, Austrelaps superbus and Pseudechis porphyriacus venoms also exhibited high myotoxicity relative to the other venoms tested. The specificity of Pseudechis porphyriacus venom was tested using the human glioma cell line TC3 and was shown to exhibit a general cytotoxicity. Myotoxicity, however, was the predominant action of the venom. It has long been known that certain animals...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom antidote; Plasma; Myotoxicity; Murine skeletal muscle; Australian elapids.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301998000100004

INTRASPECIFIC VARIATION IN NEUROTOXIC AND MYOTOXIC ACTIVITIES OF Bothrops neuwiedii VENOMS
Provedor de dados: Journal of Venomous Animals and Toxins
Autores: BORJA-OLIVEIRA,C. R.; SOARES,A. M.; ZAMUNÉR,S. R.; HYSLOP,S.; GIGLIO,J. R.; PRADO-FRANCESCHI,J.; RODRIGUES-SIMIONI,L.
Snake venoms frequently vary in composition. In this work, we compared the neurotoxic and myotoxic activities of 16 lots of Bothrops neuwiedii venoms from different regions of Brazil, using chick biventer cervicis preparations. The neuromuscular blockade varied from 2% to 100% after 120 min incubation with venoms (50 mug/ml). In all cases, this blockade was irreversible and concentration-dependent; at low concentrations (10-20 mug/ml), 15 of the 16 venom lots failed to abolish responses to acetylcholine (110 muM), but blocked responses to KCl (13.4 muM), and induced contracture. At 5-20 mug/ml, the most active venom totally blocked twitch-tension without affecting responses to acetylcholine and KCl. Polyacrylamide gel electrophoresis for basic proteins...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops neuwiedii venom; Intraspecific variation; Myotoxicity; Neurotoxicity; Neuromuscular blockade.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000100008

Non-neurotoxic activity of Malayan krait (Bungarus candidus) venom from Thailand
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Charoenpitakchai,Mongkon; Wiwatwarayos,Kulachet; Jaisupa,Nattapon; Rusmili,Muhamad Rusdi Ahmad; Mangmool,Supachoke; Hodgson,Wayne C.; Ruangpratheep,Chetana; Chanhome,Lawan; Chaisakul,Janeyuth.
Abstract  Background: Envenoming by kraits (genus Bungarus) is a medically significant issue in South Asia and Southeast Asia. Malayan krait (Bungarus candidus) venom is known to contain highly potent neurotoxins. In recent years, there have been reports on the non-neurotoxic activities of krait venom that include myotoxicity and nephrotoxicity. However, research on such non-neurotoxicity activities of Malayan krait venom is extremely limited. Thus, the aim of the present study was to determine the myotoxic, cytotoxic and nephrotoxic activities of B. candidus venoms from northeastern (BC-NE) and southern (BC-S) Thailand in experimentally envenomed rats.  Methods: Rats were administered Malayan krait (BC-NE or BC-S) venom (50 μg/kg, i.m.) or 0.9% NaCl...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Krait; Bungarus candidus; Myotoxicity; Nephrotoxicity; Venom; Kidney.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100304

Effects of manganese (Mn2+) on neurotoxic and myotoxic activities induced by Crotalus durissus terrificus and Bothrops jararacussu venoms in chick biventer cervicis preparations
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Bueno,L. G. F.; Leite,G. B.; Cruz-Höfling,M. A.; Rodrigues-Simioni,L.; Oshima-Franco,Y.
In the present study, manganese (Mn2+), a neuromuscular blocker with pre and postsynaptic actions, was used to verify the neurotoxicity and myotoxicity induced by Crotalus durissus terrificus (Cdt) and Bothrops jararacussu (Bjssu) venoms in biventer cervicis preparations (BCp). Preparations pretreated with 0.66 and 1.6mM Mn2+ did not affect Cdt venom-induced blockage nor change KCl-induced contracture but partially reduced ACh-induced contracture. However, both Mn2+ concentrations partially hindered Bjssu venom-induced blockage after washing the preparations with Krebs solution, and only 1.6mM Mn2+ preparations significantly recovered ACh-induced contracture. The effect of Cdt venom myotoxicity on contractile responses was different from that of Bjssu...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Contracture; Divalent cation; Mn2+; Myotoxicity; Neuromuscular blockage; Neurotoxicity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200007

Neuromuscular activity of Bothrops neuwiedi pauloensis snake venom in mouse nerve-muscle preparations
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Durigon,A. M.; Borja-Oliveira,C. R.; Dal Belo,C. A.; Oshima-Franco,Y.; Cogo,J. C.; Lapa,A. J.; Souccar,C.; Rodrigues-Simioni,L.
The pharmacological effects of Bothrops neuwiedi pauloensis venom on mouse phrenic nerve-diaphragm (PND) preparations were studied. Venom (20 mug/ml) irreversibly inhibited indirectly evoked twitches in PND preparations (60 ± 10% inhibition, mean ± SEM; p&lt;0.05; n=6). At 50 mug/ml, the venom blocked indirectly and directly (curarized preparations) evoked twitches in mouse hemidiaphragms. In the absence of Ca2+, venom (50 mug/ml), produced partial blockade only after an 80 min incubation, which reached 40.3 ± 7.8% (p&lt;0.05; n=3) after 120 min. Venom (20 mug/ml) increased (25 ± 2%, p&lt; 0.05) the frequency of giant miniature end-plate potentials in 9 of 10 end-plates after 30 min and the number of miniature end-plate potentials which was maximum (562 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Giant MEPPs; Myotoxicity; Neurotoxicity; Presynaptic action.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100004

Effect of suramin on myotoxicity of some crotalid snake venoms
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Arruda,E.Z.; Silva,N.M.V.; Moraes,R.A.M.; Melo,P.A.
We investigated the protective effect of suramin, an enzyme inhibitor and an uncoupler of G protein from receptors, on the myotoxic activity in mice of different crotalid snake venoms (A.c. laticinctus, C.v. viridis, C.d. terrificus, B. jararacussu, B. moojeni, B. alternatus, B. jararaca, L. muta). Myotoxicity was evaluated in vivo by injecting im the venoms (0.5 or 1.0 mg/kg) dissolved in physiological saline solution (0.1 ml) and measuring plasma creatine kinase (CK) activity. Two experimental approaches were used in mice (N = 5 for each group). In protocol A, 1 mg of each venom was incubated with 1.0 mg suramin (15 min, 37ºC, in vitro), and then injected im into the mice at a dose of 1.0 mg/kg (in vivo). In protocol B, venoms, 1.0 mg/kg, were injected...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Snake venom; Myotoxicity; Creatine kinase; Suramin.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600013

Effect of perimuscular injection of Bothrops jararacussu venom on plasma creatine kinase levels in mice: influence of dose and volume
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Calil-Elias,S.; Thattassery,E.; Martinez,A.M.B.; Melo,P.A.
The effect of dose and volume of a perimuscular injection of Bothrops jararacussu venom on myonecrosis of skeletal muscle was studied in mice. An increase of the venom dose (0.25 to 2.0 µg/g) at a given volume (50 µl) resulted in an increase in plasma creatine kinase (CK) levels 2 h after injection. Plasma CK activity increased from the basal level of 129.27 ± 11.83 (N = 20) to 2392.80 ± 709.43 IU/l (N = 4) for the 1.0 µg/g dose. Histological analysis of extensor digitorum longus muscle 4 h after injection showed lesion of peripheral muscle fibers, disorganization of the bundles or the complete degeneration of muscle fibers. These lesions were more extensive when higher doses were injected. Furthermore, an increase in volume (12.5 to 100 µl) by dilution of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bothrops venoms; Myotoxicity; Skeletal muscle; Plasma creatine kinase.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000018

The pharmacological effect of Bothrops neuwiedii pauloensis (jararaca-pintada) snake venom on avian neuromuscular transmission
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Borja-Oliveira,C.R.; Durigon,A.M.; Vallin,A.C.C.; Toyama,M.H.; Souccar,C.; Marangoni,S.; Rodrigues-Simioni,L.
The neuromuscular effects of Bothrops neuwiedii pauloensis (jararaca-pintada) venom were studied on isolated chick biventer cervicis nerve-muscle preparations. Venom concentrations of 5-50 µg/ml produced an initial inhibition and a secondary increase of indirectly evoked twitches followed by a progressive concentration-dependent and irreversible neuromuscular blockade. At venom concentrations of 1-20 µg/ml, the responses to 13.4 mM KCl were inhibited whereas those to 110 µM acetylcholine alone and cumulative concentrations of 1 µM to 10 mM were unaffected. At venom concentrations higher than 50 µg/ml, there was pronounced muscle contracture with inhibition of the responses to acetylcholine, KCl and direct stimulation. At 20-24ºC, the venom (50 µg/ml)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chick biventer cervicis; Myotoxicity; Neurotoxicity; Phospholipase A2; Presynaptic action.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500009

Pulsed ultrasound therapy accelerates the recovery of skeletal muscle damage induced by Bothrops jararacussu venom
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Saturnino-Oliveira,J.; Tomaz,M.A.; Fonseca,T.F.; Gaban,G.A.; Monteiro-Machado,M.; Strauch,M.A.; Cons,B.L.; Calil-Elias,S.; Martinez,A.M.B.; Melo,P.A.
We studied the effect of pulsed ultrasound therapy (UST) and antibothropic polyvalent antivenom (PAV) on the regeneration of mouse extensor digitorum longus muscle following damage by Bothrops jararacussu venom. Animals (Swiss male and female mice weighing 25.0 ± 5.0 g; 5 animals per group) received a perimuscular injection of venom (1 mg/kg) and treatment with UST was started 1 h later (1 min/day, 3 MHz, 0.3 W/cm², pulsed mode). Three and 28 days after injection, muscles were dissected and processed for light microscopy. The venom caused complete degeneration of muscle fibers. UST alone and combined with PAV (1.0 mL/kg) partially protected these fibers, whereas muscles receiving no treatment showed disorganized fascicules and fibers with reduced diameter....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulsed therapeutic ultrasound; Bothrops snake venom; Myotoxicity; Mouse skeletal-muscle regeneration.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600003