Consulta: subjectFacets:"Myotoxins"
Registros recuperados: 4
Data/hora: 07/06/2024 11:35:54

Neutralization of pharmacological and toxic activities of Bothrops jararacussu snake venom and isolated myotoxins by Serjania erecta methanolic extract and its fractions
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Fernandes,RS; Costa,TR; Marcussi,S; Bernardes,CP; Menaldo,DL; Rodriguéz Gonzaléz,II; Pereira,PS; Soares,AM.
Most of the snakebites recorded in Brazil are caused by the Bothrops genus. Given that the local tissue damage caused by this genus cannot be treated by antivenom therapy, numerous studies are focusing on supplementary alternatives, such as the use of medicinal plants. Serjania erecta has already demonstrated anti-inflammatory, antiseptic and healing properties. In the current study, the aerial parts of S. erecta were extracted with methanol, then submitted to chromatographic fractionation on a Sephadex LH20 column and eluted with methanol, which resulted in four main fractions. The crude extract and fractions neutralized the toxic activities of Bothrops jararacussu snake venom and isolated myotoxins (BthTX-I and II). Results showed that phospholipase A2,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plants; Antiophidian properties; Serjania erecta; Bothrops jararacussu snake venom; Myotoxins.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100011

Anti-inflammatory activity of Blutaparon portulacoides ethanolic extract against the inflammatory reaction induced by Bothrops jararacussu venom and isolated myotoxins BthTX-I and II
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Pereira,IC; Barbosa,AM; Salvador,MJ; Soares,AM; Ribeiro,W; Cogo,JC; Zamuner,SR.
This article reports the anti-inflammatory effect of Blutaparon portulacoides (B. portulacoides), specifically the ethanolic extract of its aerial parts, on the edema formation and leukocyte influx caused by Bothrops jararacussu (B. jararacussu) snake venom and Bothropstoxin-I and II (BthTX-I and II) isolated from this venom as an alternative treatment for Bothrops snakebites. The anti-inflammatory effect of B. portulacoides ethanolic extract was compared with an animal group pretreated with dexamethasone. B. portulacoides ethanolic extract significantly inhibited paw edema induced by B. jararacussu venom and by BthTX-I and II. Also, results demonstrated that the extract caused a reduction of the leukocyte influx induced by BthTX-I. However, the extract...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararacussu; Myotoxins; Inflammation; Edema; Blutaparon portulacoides.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300013

Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Barbosa,AM; Villaverde,AB; Guimarães-Sousa,L; Soares,AM; Zamuner,SF; Cogo,JC; Zamuner,SR.
The prominent myotoxic effects induced by Bothrops jararacussu crude venom are due, in part, to its polycationic myotoxins, BthTX-I and BthTX-II. Both myotoxins have a phospholipase A2 structure: BthTX-II is an active enzyme Asp-49 PLA2, while BthTX-I is a Lys-49 PLA2 devoid of enzymatic activity. In this study, the effect of low-level laser therapy (LLLT), 685 nm laser at a dose of 4.2 J/cm2 on edema formation, leukocyte influx and myonecrosis caused by BthTX-I and BthTX-II, isolated from Bothrops jararacussu snake venom, was analyzed. BthTX-I and BthTX-II caused a significant edema formation, a prominent leukocyte infiltrate composed predominantly by neutrophils and myonecrosis in envenomed gastrocnemius muscle. LLLT significantly reduced the edema...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararacussu; Myotoxins; Inflammation; Myonecrosis; Low-level laser therapy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300014

A single-step purification of bothropstoxin-1
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Spencer,P.J.; Aird,S.D.; Boni-Mitake,M.; Nascimento,N.; Rogero,J.R.
Bothrops venoms are complex mixtures of components with a wide range of biological activities. Among these substances, myotoxins have been investigated by several groups. Bothropstoxin-1 (Bthtx-1) is a phospholipase A2-like basic myotoxin from Bothrops jararacussu. The purification of this component involves two chromatographic steps. Although providing a pure material, the association of these two steps is time consuming and a single-step method using high performance chromatography media would be useful. In the present study, we describe a single-step purification method for Bthtx-1. Bothrops jararacussu venom was dissolved in 1 ml buffer. After centrifugation, the supernatant was injected into a Resource-S cation exchange column connected to an FPLC...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Myotoxins; Purification; Chromatography; Bothrops jararacussu; Bothropstoxin-1.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900004