Consulta: subjectFacets:"Neutral red retention assay"
Registros recuperados: 2
Data/hora: 06/06/2024 05:28:10

Physiological and cellular responses in two populations of the mussel Perna perna collected at different sites from the coast of São Paulo, Brazil
Provedor de dados: 52
Autores: Abessa,Denis Moledo de Souza; Zaroni,Letícia Pires; Sousa,Eduinetty Ceci Pereira Moreira de; Gasparro,Marcia Regina; Pereira,Camilo Dias Seabra; Rachid,Bauer Rodarte de Figueredo; Depledge,Michael; King,Rebecca Susan.
The physiological conditions of mussels from Ubatuba and Santos and also of organisms transplanted from Ubatuba to Santos were studied by using different techniques. Assays for lysosomal stability were conducted on the haemolymph. Heart rate activity was monitored for 6h. The embryonic development of larvae obtained from the collected mussels was analysed. For all the compared groups of mussels, no significant differences were observed for the cardiac activity monitoring and the embryonic bioassays. The mean Neutral Red (NR) retention time was similar for the animals from Santos and Ubatuba, whereas the organisms transplanted to Santos showed a reduction in the retention time of the dye, indicating damage in the lysosomal membranes. These differences were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Perna perna; Neutral red retention assay; Capmon system; Embryonic assay; Biomarkers; Physiology.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200008

Copper Induced Lysosomal Membrane Destabilisation in Haemolymph Cells of Mediterranean Green Crab (Carcinus aestuarii, Nardo, 1847) from the Narta Lagoon (Albania)
Provedor de dados: 52
Autores: Aliko,Valbona; Hajdaraj,Gerta; Caci,Atenisa; Faggio,Caterina.
ABSTRACTDestabilisation of blood cell lysosomes in Mediterranean green crabCarcinus aestuarii was investigated using Neutral Red Retention Assay (NRRA). Crabs collected in Narta Lagoon, Vlora (Albania) during May 2014 were exposed in the laboratory to sub-lethal, environmentally realistic concentrations of copper. Neutral Red Retention Time (NRRT) and glucose concentration in haemolymph of animals were measured. The mean NRRT showed a significant reduction for the animals of the treatment group compared to the control one (from 118.6 ± 28.4 to 36.4 ± 10.48 min, p&lt;0.05), indicating damage of lysosomal membrane. Haemolymph glucose concentration was significantly higher in the treatment group (from 37.8 ± 2.7 to 137.8.4 ± 16.2 mg/dL, p&lt;0.05) than in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carcinus aestuarii; Neutral red retention assay; Lysosomal membrane destabilization; Bioindicator; Copper.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500750