Consulta: subjectFacets:"Non-homologous DNA end joining"
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Data/hora: 10/06/2024 18:15:05

Non-homologous DNA end joining in normal and cancer cells and its dependence on break structures
Provedor de dados: Genetics and Molecular Biology
Autores: Poplawski,Tomasz; Pastwa,Elzbieta; Blasiak,Janusz.
DNA double-strand breaks (DSBs) are a serious threat to the cell, for if not or miss-repaired, they can lead to chromosomal aberration, mutation and cancer. DSBs in human cells are repaired via non-homologous DNA end joining (NHEJ) and homologous recombination repair pathways. In the former process, the structure of DNA termini plays an important role, as does the genetic constitution of the cells, through being different in normal and pathological cells. In order to investigate the dependence of NHEJ on DSB structure in normal and cancer cells, we used linearized plasmids with various, complementary or non-complementary, single-stranded or blunt DNA termini, as well as whole-cell extract isolated from normal human lymphocytes, chronic myeloid leukemia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA repair; Non-homologous DNA end joining; DNA double-strand breaks; Complementary and non-complementary DNA ends.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200027