Consulta: subjectFacets:"Non-small cell lung carcinoma"
Registros recuperados: 2
Data/hora: 04/06/2024 08:15:00

Analysis of gene expression changes associated with human carcinoma-associated fibroblasts in non-small cell lung carcinoma
Provedor de dados: Biological Research
Autores: Wu,Xiaofen; Ruan,Lei; Yang,Yi; Mei,Qi.
Abstract  Background  This study aimed to investigate the gene expression changes associated with carcinoma-associated fibroblasts (CAFs) involving in non-small cell lung carcinoma (NSCLC).  Methods  We downloaded the GEO series GSE22862, which contained matched gene expression values for 15 CAF and normal fibroblasts samples, and series GSE27289 containing SNP genotyping for four matched NSCLC samples. The differentially expressed genes in CAF samples were identified using the limma package in R. Then we performed gene ontology (GO) and pathway enrichment analysis and protein&amp;#8211;protein interaction (PPI) network construction using the identified DEGs. Moreover, aberrant cell fraction, ploidy, allele-specific copy number, and loss of heterozygosity...
Tipo: Journal article
Palavras-chave: Carcinoma-associated fibroblast; Non-small cell lung carcinoma; Differentially expressed genes; Allele-specific copy number; Functional analysis.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100204

Intercellular contact-dependent survival of human A549, NCI-H596 and NCI-H520 non-small cell lung carcinoma cell lines
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Castro,M.A.A.; Schwartsmann,G.; Moreira,J.C.F.
In the present study, we examined the relationship between cell phenotype and cell survival of three human non-small cell lung carcinoma cell lines (A549, NCI-H596 and NCI-H520). Cells in exponential growth at various densities were incubated for 24 h at 37ºC in a 5% CO2 humidified atmosphere and then exposed to UV radiation for 1 min (256 nm, 40 W, source-to-target distance 100 cm). After two days the surviving cells were quantified by sulforhodamine ß staining and DNA fragmentation assay. The differences in UV sensitivity at 60 x 10³ cells/cm² among the cell lines were not related to the proliferative state of the cells but to the extent of intercellular contact. In contrast to A549 and NCI-H596, irradiated NCI-H520 cells presented lower DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-small cell lung carcinoma; Cell density; Intercellular contact; Cell phenotype; UV radiation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800006