Consulta: subjectFacets:"Norovirus"
Registros recuperados: 40
Data/hora: 03/06/2024 16:33:23

Metagenomic to evaluate norovirus genomic diversity in oysters: Impact on hexamer selection and targeted capture-based enrichment
Provedor de dados: 5
Autores: Strubbia, Sofia; Schaeffer, Julien; Besnard, Alban; Wacrenier, Candice; Le Mennec, Cecile; Garry, Pascal; Desdouits, Marion; Le Guyader, Soizick.
Human virus transmission through food consumption has been identified since many years and the international trade increases the risk of dissemination of viral pathogens. The development of metagenomic approach holds many promises for the surveillance of viruses in food and water. This work aimed to analyze norovirus diversity and to evaluate strain-dependent accumulation patterns in three oyster types by using a metagenomic approach. Different hexamer sets to prime cDNA were evaluated before capture-based approach to enhance virus reads recovery during deep sequencing. The study includes the use of technical replicates of artificially contaminated oysters and the analysis of multiple negatives controls. Results showed a clear impact of the hexamer set...
Tipo: Text
Palavras-chave: Norovirus; Oysters; Metagenomic; Human enteric viruses.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00614/72575/71535.pdf

Detection of multiple noroviruses associated with an international gastroenteritis outbreak linked to oyster consumption
Provedor de dados: 5
Autores: Le Guyader, Soizick; Bon, Fabienne; Demedici, Dario; Parnaudeau, Sylvain; Bertone, Alessandra; Crudeli, Silvia; Doyle, Aoife; Zidane, Mohamed; Suffredini, Elisabetta; Kohli E, Evelyne; Maddalo, Francesco; Monini, Marina; Gallay, Anne; Pommepuy, Monique; Pothier, Pierre; Ruggeri, Franco M.
An international outbreak linked to oyster consumption involving a group of over 200 people in Italy and 127 total subjects in 13 smaller clusters in France was analyzed using epidemiological and clinical data and shellfish samples. Environmental information from the oyster-producing area, located in a lagoon in southern France, was collected to investigate the possible events leading to the contamination. Virologic analyses were conducted by reverse transcription-PCR (RT-PCR) using the same primer sets for both clinical and environmental samples. After sequencing, the data were analyzed through the database operated by the scientific network FoodBorne Viruses in Europe. The existence of an international collaboration between laboratories was critical to...
Tipo: Text
Palavras-chave: Seafood; Contamination; Norovirus; Epidemiology; Virology; Oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2096.pdf

Evaluation of various real-time RT-PCR assays for the detection and quantitation of human norovirus
Provedor de dados: 5
Autores: Butot, Sophie; Le Guyader, Soizick; Krol, Joanna; Putallaz, Thierry; Amoroso, Richard; Sanchez, Gloria.
Human noroviruses (NoVs) are the most common viruses causing acute gastroenteritis in humans. Performance characteristics of two commercial quantitative NoV RT-PCR assays, the Norovirus real-time RT-PCR Kit (AnDiaTec) and the Type I and Type II kits (Generon), and the international assay as selected by the CEN/TC/WG6/TAG4 group were evaluated for the specific detection and quantitation of 59 NoV samples, including different subtypes of NoV genogroup I and II. The results showed that the method proposed by the CEN/TC/WG6/TAG4 group was 100% specific since it was able to detect all samples tested. The commercialized kits evaluated failed to detect a vast majority of NoV GI strains. Additionally the Generon kit did not succeed to detect strains from GII.3,...
Tipo: Text
Palavras-chave: Norovirus; Real-time RT-PCR; Commercial kit.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00006/11760/8473.pdf

Les Toxi-Infections Alimentaires Collectives (TIAC) virales associées à la consommation d'huîtres en France, hiver 2000-2001
Provedor de dados: 5
Autores: Zidane, Mohamed; Le Guyader, Soizick; Pommepuy, Monique.
During the winter 2000/2001, several outbreaks associated with the consumption of shellfish were declared in France. Following these events, a close cooperation was organized between administrations and laboratories (DGAL, DPMA, InVS, AFSSA, Ifremer, CHU-Dijon and CNC) to gather competences and to manage the situation. This report synthesizes the information collected during these outbreaks.  From the outset, the epidemiologic investigations directed the research towards viral contamination and shellfish as contamination source. Several outbreaks were investigated. Oysters and stools samples from ill patients were analysed by RT-PCR for viruses (enterovirus, rotavirus, norovirus and astrovirus). The genic amplification was done by using the same primers in...
Tipo: Text
Palavras-chave: Épidémie; Gastro-entérites; Huîtres; Contamination virale; RT-PCR; Norovirus; TIAC.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/00068/17941/15476.pdf

Rapport d'activités 2020. Laboratoire Santé Environnement et Microbiologie, Laboratoire National de Référence de Microbiologie des coquillages
Provedor de dados: 5
Autores: Garry, Pascal; Le Guyader, Soizick.
Ce rapport présente une synthèse des actions, et travaux réalisés par le laboratoire Santé Environnement et Microbiologie pendant l’année 2020. Les divers projets de recherche sont résumés et les derniers développements ou résultats sont brièvement évoqués. Les actions en tant que Laboratoire National de Référence pour la Microbiologie des Coquillages et les activités pour la coordination du réseau REMI sont également présentées.
Tipo: Text
Palavras-chave: Microbiologie sanitaire; Bactéries entériques; Vibrions; Virus entériques humains; Norovirus; SARS-CoV-2; Coquillages. Activité de référence; REMI.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00691/80332/83455.pdf

Norovirus contamination on French marketed oysters
Provedor de dados: 5
Autores: Schaeffer, Julien; Le Saux, Jean-claude; Lora, Monica; Atmar, Robert; Le Guyader, Soizick.
Contaminaded shellfish have been implicated in gastroenteritis outbreaks in different countries. As no regulation has been set up yet regarding viral contamination of food, very few data are available on the prevalence of contamined products on the market. This study presents data obtained from oysters collected on the French market in one producing area over a 16 month period of time. Noroviruses were detected in 9% of samples with a seasonal impact and influence of climatic events. Contamination levels were low and, surprisingly, oysters sampled directly from the producer were found to have less contamination than oystes from supermarkets.
Tipo: Text
Palavras-chave: Oyster; Marker; Norovirus; Quantification; Calicivirus; Prevalence.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00152/26367/24457.pdf

Microbiologie et coquillages : des enjeux et des développements futurs
Provedor de dados: 5
Autores: Le Guyader, Soizick; Garry, Pascal; Ollivier, Joanna; Piquet, Jean-come.
The shellfish safety has long been considered a public health problem. The implementation of a regulation based on a bacterial indicator of faecal contamination has significantly reduced outbreaks of bacterial origin. However, epidemics of viral gastroenteritis due to the presence of noroviruses from human sewage persist. We demonstrated few years ago that oysters are not just a passive filter but are able to select some viral strains, via the presence of specific ligands, very similar to ligands observed in humans for these same virus. Ongoing developments, in conjunction with the application of metagenomic tools, will allow us to better understand the fate of some human pathogens after their release into the coastal environment.
Tipo: Text
Palavras-chave: Shellfish; Microbiology; Oysters; Norovirus.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00599/71141/69457.pdf

Scientific Opinion on an update on the present knowledge on the occurrence and control of foodborne viruses
Provedor de dados: 5
A review of the biology, epidemiology, diagnosis and public health importance of foodborne viruses was performed. Data needs to support a risk assessment were also identified. In addition possible control options and their anticipated impact to prevent or reduce the number of foodborne viral human infections were identified, including the scientific reasons for and against the establishment of food safety criteria and process hygiene criteria for viruses for certain food categories. Food may be contaminated by virus during all stages of the food supply chain, and transmission can occur by consumption of food contaminated during the production process (primary production, or during further processing), or contaminated by infected food handlers. Transmission...
Tipo: Text
Palavras-chave: Food borne viruses; Norovirus; Hepatitis; Microbiological criteria; Molluscs; Fresh produce.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00066/17734/15255.pdf

Rapport d’activités 2019. Laboratoire Santé Environnement et Microbiologie, Laboratoire National de Référence de Microbiologie des coquillages
Provedor de dados: 5
Autores: Garry, Pascal; Le Guyader, Soizick.
Ce rapport présente une synthèse des actions, et travaux réalisés par le laboratoire Santé Environnement et Microbiologie pendant l’année 2019. Les divers projets de recherche sont résumés et les derniers développements ou résultats sont brièvement évoqués. Les actions en tant que Laboratoire National de Référence pour la Microbiologie des Coquillages et les activités pour la coordination du réseau REMI sont également présentées.
Tipo: Text
Palavras-chave: Microbiologie sanitaire; Bactéries entériques; Vibrions; Virus entériques humains; Norovirus; Coquillage. Activité de référence; REMI.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00615/72671/71688.pdf

Infectivity of GI and GII noroviruses established from oyster related outbreaks
Provedor de dados: 5
Autores: Thebault, Anne; Teunis, Peter F. M.; Le Pendu, Jacques; Le Guyader, Soizick; Denis, Jean-baptiste.
Noroviruses (NoVs) are the major cause of acute epidemic gastroenteritis in industrialized countries. Outbreak strains are predominantly genogroup II (GII) NoV, but genogroup I (GI) strains are regularly found in oyster related outbreaks. The prototype Norwalk virus (GI), has been shown to have high infectivity in a human challenge study. Whether other NoVs are equally infectious via natural exposure remains to be established. Human susceptibility to NoV is partly determined by the secretor status (Se+/-). Data from five published oyster related outbreaks were analyzed in a Bayesian framework. Infectivity estimates where high and consistent with NV(GI) infectivity, for both GII and GI strains. The median and CI95 probability of infection and illness, in...
Tipo: Text
Palavras-chave: Norovirus; Shellfish; Bayesian analysis; Dose-response relationship; Fucosyltransferases.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00148/25927/24030.pdf

Metavirome Sequencing to Evaluate Norovirus Diversity in Sewage and Related Bioaccumulated Oysters
Provedor de dados: 5
Autores: Strubbia, Sofia; Schaeffer, Julien; Oude Munnink, Bas B.; Besnard, Alban; Phan, My V. T.; Nieuwenhuijse, David F.; De Graaf, Miranda; Schapendonk, Claudia M. E.; Wacrenier, Candice; Cotten, Matthew; Koopmans, Marion P. G.; Le Guyader, Soizick.
Metagenomic sequencing is a promising method to determine the virus diversity in environmental samples such as sewage or shellfish. However, to identify the short RNA genomes of human enteric viruses among the large diversity of nucleic acids present in such complex matrices, method optimization is still needed. This work presents methodological developments focused on norovirus, a small ssRNA non-enveloped virus known as the major cause of human gastroenteritis worldwide and frequently present in human excreta and sewage. Different elution protocols were applied and Illumina MiSeq technology were used to study norovirus diversity. A double approach, agnostic deep sequencing and a capture-based approach (VirCapSeq-VERT) was used to identify norovirus in...
Tipo: Text
Palavras-chave: Norovirus; Sewage; Oysters; Metagenomic sequencing; Metavirome.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00586/69765/67656.pdf

A Targeted Metagenomics Approach to Study the Diversity of Norovirus GII in Shellfish Implicated in Outbreaks
Provedor de dados: 5
Autores: Desdouits, Marion; Wacrenier, Candice; Ollivier, Joanna; Schaeffer, Julien; Le Guyader, Soizick.
Human noroviruses (NoV) cause epidemics of acute gastroenteritis (AGE) worldwide and can be transmitted through consumption of contaminated foods. Fresh products such as shellfish can be contaminated by human sewage during production, which results in the presence of multiple virus strains, at very low concentrations. Here, we tested a targeted metagenomics approach by deep-sequencing PCR amplicons of the capsid (VP1) and polymerase (RdRp) viral genes, on a set of artificial samples and on shellfish samples associated to AGE outbreaks, to evaluate its advantages and limitations in the identification of strains from the NoV genogroup (G) II. Using artificial samples, the method allowed the sequencing of most strains, but not all, and displayed variability...
Tipo: Text
Palavras-chave: Norovirus; Foodborne virus; Metagenomics; Amplicon deep sequencing; Viral diversity.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00646/75831/76818.pdf

Devenir des virus entériques humains en milieu marin : Apport des VLPs (Virus Like Particles) pour la purification des coquillages
Provedor de dados: 5
Autores: Loisy, Fabienne.
This work was developed following two research axes: the improvement of norovirus (human and animal strain) and rotavirus detection, and the evaluation of VLPs as a viral substitute for studying their comportment in marine environment. The main point concerning the method optimisation was the development of real-time RT-PCR for norovirus detection. The second part of the work has demonstrated the potential of norovirus and rotavirus VLPs as a viral surrogate as proven by the study of their stability in seawater. As VLPs are non-pathogen particles, the long persistence of rotavirus VLPs in shellfish has been shown in natural conditions (oysters on marine estuaries). The studies of Norwalk virus VLPs attachment to oyster tissues have demonstrated a specific...
Tipo: Text
Palavras-chave: Specific binding; Persistence; Shellfish; Real time RT PCR; VLPs; Rotavirus; Norovirus; Fixation spécifique; Persistance; Coquillage; RT PCR en temps réel; VLPs; Rotavirus.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/these-327.pdf

Contamination des coquillages par les norovirus en rivière d'Auray (Norocoqauray)
Provedor de dados: 5
Autores: Treguier, Cathy; Schaeffer, Julien; Le Saux, Jean-claude; Cochennec-laureau, Nathalie; Le Guyader, Soizick.
Les norovirus (NoV) sont responsables des épidémies hivernales de gastroentérites aiguës dans la population. Ils sont excrétés dans les fèces humains, puis se retrouvent, après épuration insuffisante, dans le milieu marin naturel où ils peuvent contaminer des coquillages, qui à leur tour risquent de déclencher des gastroentérites chez les consommateurs. Cette étude vise à améliorer les connaissances sur la contamination des coquillages en élevage en rivière d’Auray par les NoV, en lien avec les bactéries témoins de contamination fécale Escherichia coli (E. coli). L’objectif est de suivre les flux de NoV et d’E. coli apportés par différents rejets (dont une station d’épuration) impactant la zone conchylicole et leur influence sur la qualité des coquillages...
Tipo: Text
Palavras-chave: Norovirus; Coquillages; Contamination; Station d’épuration; Flux; Rivière d’Auray; Escherichia coli.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00267/37778/35829.pdf

Huîtres et norovirus : une affinité particulière?
Provedor de dados: 5
Autores: Le Guyader, Soizick; Maalouf, Haifa; Le Pendu, Jacques.
Shellfish can be a vector for human pathogens. Despite regulation based on enteric bacteria, shellfish are still implicated in viral outbreaks. Oysters are the most common shellfish associated with outbreaks, and noroviruses, which cause acute gastroenteritis, are the most frequently identified pathogen in these outbreaks. Analysis of shellfish-related outbreak data worldwide shows an unexpected high proportion of genogroup I strains. Recent studies performed in vitro, in vivo and in the environment indicate that oysters are not just a passive filter, but can selectively accumulate norovirus strains based on virus carbohydrate ligands shared with humans. These observations may help explain the GI/GII bias observed in shellfish-related outbreaks compared to...
Tipo: Text
Palavras-chave: Huîtres; Norovirus; Ligand; Sélection de souches; Oysters; Norovirus; Ligand; Strain selection.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00152/26357/24514.pdf

Characterization of Norovirus and Other Human Enteric Viruses in Sewage and Stool Samples Through Next-Generation Sequencing
Provedor de dados: 5
Autores: Strubbia, Sofia; Phan, My V. T.; Schaeffer, Julien; Koopmans, Marion; Cotten, Matthew; Le Guyader, Soizick.
This study aimed to optimize a method to identify human enteric viruses in sewage and stool samples using random primed next-generation sequencing. We tested three methods, two employed virus enrichment based on the binding properties of the viral capsid using pig-mucin capture or by selecting viral RNA prior to library preparation through a capture using the SureSelect target enrichment. The third method was based on a non-specific biophysical precipitation with polyethylene glycol. Full genomes of a number of common human enteric viruses including norovirus, rotavirus, husavirus, enterovirus and astrovirus were obtained. In stool samples full norovirus genome were detected as well as partial enterovirus genome. A variety of norovirus sequences was...
Tipo: Text
Palavras-chave: Human enteric viruses; Norovirus; Sewage; Metagenomic; Virome.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00512/62332/66592.pdf

Norovirus and Other Human Enteric Viruses in Moroccan Shellfish
Provedor de dados: 5
Autores: Benabbes, Laila; Ollivier, Joanna; Schaeffer, Julien; Parnaudeau, Sylvain; Rhaissi, Houria; Nourlil, Jalal; Le Guyader, Soizick.
The aim of this study was to evaluate the presence of human enteric viruses in shellfish collected along the Mediterranean Sea and Atlantic Coast of Morocco. A total of 77 samples were collected from areas potentially contaminated by human sewage. Noroviruses were detected in 30 % of samples, with an equal representation of GI and GII strains, but were much more frequently found in cockles or clams than in oysters. The method used, including extraction efficiency controls, allowed the quantification of virus concentration. As in previous reports, results showed levels of contamination between 100 and 1,000 copies/g of digestive tissues. Sapoviruses were detected in 13 % of samples mainly in oyster and clam samples. Hepatitis A virus was detected in two...
Tipo: Text
Palavras-chave: Shellfish; Norovirus; Hepatitis A virus; Environmental conditions; Quantification; Morocco.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00130/24102/22489.pdf

Assessment of human enteric viruses in cultured and wild bivalve molluscs
Provedor de dados: 5
Autores: Luz Vilarino, M.; Le Guyader, Soizick; Polo, David; Schaeffer, Julien; Krol, Joanna; Romalde, Jesus L.
Standard and real-time reverse transcription-PCR (rRT-PCR) procedures were used to monitor cultured and wild bivalve molluscs from the Ria de Vigo (NW Spain) for the main human enteric RNA viruses, specifically, norovirus (NoV), hepatitis A virus (HAV), astrovirus (AsV), rotavirus (RT), enterovirus (EV), and Aichi virus (AiV). The results showed the presence of at least one enteric virus in 63.4% of the 41 samples analyzed. NoV GII was the most prevalent virus, detected in 53.7% of the samples, while NoV GI, AsV, EV, and RV were found at lower percentages (7.3, 12.2, 12.2, and 4.9%, respectively). In general, samples obtained in the wild were more frequently contaminated than those from Cultured (70.6 vs. 58.3%) molluscs and were more readily contaminated...
Tipo: Text
Palavras-chave: Seafood industry; Viral quantification; Viral prevalence; Norovirus; Hepatitis A virus; Enteric viruses; Molluscs.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6957.pdf

Validation of EN ISO method 15216-Part 1-Quantification of hepatitis A virus and norovirus in food matrices
Provedor de dados: 5
Autores: Lowther, J. A.; Bosch, A.; Butot, S.; Ollivier, Joanna; Maede, D.; Rutjes, S. A.; Hardouin, G.; Lombard, B.; In'T Veld, P.; Leclercq, A.
Hepatitis A virus (HAV) and norovirus are important agents of food-borne human viral illness, with common vehicles including bivalve molluscan shellfish, soft fruit and various vegetables. Outbreaks of viral illness due to contamination of the surfaces of foods, or food preparation surfaces by for example infected food handlers are also common. Virus analysis of food matrices can contribute towards risk management for these hazards and a two-part technical specification for determination of Hepatitis A virus and norovirus in food matrices (ISO/TS 15216:2013) was published jointly by the European Committee for Standardisation and the International Organization for Standardization in 2013. As part of the European Mandate No. M381 to validate 15 standards in...
Tipo: Text
Palavras-chave: Norovirus; Hepatitis A virus; Real-time RT-PCR; Validation; Standardization; Bivalve molluscan shellfish; Soft fruit; Vegetables; Bottled water; Surfaces.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00476/58776/61442.pdf

Novel opportunities for NGS-based one health surveillance of foodborne viruses
Provedor de dados: 5
Autores: Desdouits, Marion; De Graaf, Miranda; Strubbia, Sofia; Oude Munnink, Bas B.; Kroneman, Annelies; Le Guyader, Soizick; Koopmans, Marion P. G.
Foodborne viral infections rank among the top 5 causes of disease, with noroviruses and hepatitis A causing the greatest burden globally. Contamination of foods by infected food handlers or through environmental pollution are the main sources of foodborne illness, with a lesser role for consumption of products from infected animals. Viral partial genomic sequencing has been used for more than two decades to track foodborne outbreaks and whole genome or metagenomics next-generation-sequencing (NGS) are new additions to the toolbox of food microbiology laboratories. We discuss developments in the field of targeted and metagenomic NGS, with an emphasis on application in food virology, the challenges and possible solutions towards future routine application
Tipo: Text
Palavras-chave: Next-generation sequencing; Metagenomics; Foodborne virus; Food virology; Human enteric virus; Norovirus.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00641/75330/75988.pdf

Processing Strategies to Inactivate Enteric Viruses in Shellfish
Provedor de dados: 5
Autores: Richards, Gary P.; Mcleod, Catherine; Le Guyader, Soizick.
Noroviruses, hepatitis A and E viruses, sapovirus, astrovirus, rotavirus, Aichi virus, enteric adenoviruses, poliovirus, and other enteroviruses enter shellfish through contaminated seawater or by contamination during handling and processing, resulting in outbreaks ranging from isolated to epidemic. Processing and disinfection methods include shellfish depuration and relaying, cooking and heat pasteurization, freezing, irradiation, and high pressure processing. All the methods can improve shellfish safety; however, from a commercial standpoint, none of the methods can guarantee total virus inactivation without impacting the organoleptic qualities of the shellfish. Noroviruses cause the majority of foodborne viral illnesses, yet there is conflicting...
Tipo: Text
Palavras-chave: Shellfish; Processing; Norovirus; Hepatitis A virus; Depuration; High pressure; Heat inactivation.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00011/12253/9199.pdf

Virus Type-Specific Removal in a Full-Scale Membrane Bioreactor Treatment Process
Provedor de dados: 5
Autores: Miura, Takayuki; Schaeffer, Julien; Le Saux, Jean-claude; Le Mehaute, Philippe; Le Guyader, Soizick.
We investigated removal of noroviruses, sapoviruses, and rotaviruses in a full-scale membrane bioreactor (MBR) plant by monitoring virus concentrations in wastewater samples during two gastroenteritis seasons and evaluating the adsorption of viruses to mixed liquor suspended solids (MLSS). Sapoviruses and rotaviruses were detected in 25% of MBR effluent samples with log reduction values of 3- and 2-logs in geometric mean concentrations, respectively, while noroviruses were detected in only 6% of the samples. We found that norovirus and sapovirus concentrations in the solid phase of mixed liquor samples were significantly higher than in the liquid phase (P &lt; 0.01, t test), while the concentration of rotaviruses was similar in both phases. The efficiency...
Tipo: Text
Palavras-chave: Norovirus; Sapovirus; Rotavirus; MBR; Mixed liquor suspended solids; Adsorption.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00414/52548/53441.pdf

Prévalence des norovirus dans les huîtres mises sur le marché dans le Département de la Vendée. Période février 2010-mai 2011
Provedor de dados: 5
Autores: Le Saux, Jean-claude; Lora, M.; Schaeffer, Julien; Poiraud, G.; Le Guyader, Soizick.
L’objet de cette étude est d’évaluer, par les méthodes de détection virale en cours de validation au niveau européen, le niveau de présence des norovirus dans les huîtres à l’expédition et sur les lieux de vente dans un département test, celui de la Vendée. En effet, aujourd’hui peu d’informations existent au niveau international et national sur la prévalence des norovirus dans les coquillages mis dans les circuits de consommation, alors que tous les ans des coquillages, répondant aux critères de mise en marché, sont incriminés ou suspectés dans des foyers de toxi-infections alimentaires collectives liés à la consommation de coquillages, majoritairement contaminés par des virus et plus particulièrement par les norovirus. Cette étude sur la période de...
Tipo: Text
Palavras-chave: Microbiologie; Coquillages; Norovirus; Huître creuse; Détection; Santé publique; Département Vendée.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00185/29650/28019.pdf

Characterization of new recombinant noroviruses
Provedor de dados: 5
Autores: Ambert Balay, K; Bon, F; Le Guyader, Soizick; Pothier, P; Kohli, E.
Noroviruses are important etiologic agents of acute gastroenteritis and show great genetic diversity. To characterize more fully previously detected strains that could not be assigned unequivocally to one particular genotype based on the RNA polymerase, we have sequenced a region in the capsid gene and, in some cases, in the junction between open reading frame 1 (ORF1) and ORF2. The results allowed us to identify several recombinant noroviruses: GGHb viruses were detected for the first time in France in August 2000 and then spread through France and to Europe during the following winter. Here we present the characterization of three other probable GII recombinants which showed different phylogenetic positions depending on their ORF1 and ORF2 sequences....
Tipo: Text
Palavras-chave: Genogroup; RNA recombination; Gastroenteritis; Round structured virus; Reverse transcription; Norovirus.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-1234.pdf

A large multi-pathogen waterborne community outbreak linked to faecal contamination of a groundwater system, France, 2000
Provedor de dados: 5
Autores: Gallay, A; De Valk, H; Cournot, M; Ladeuil, B; Hemery, C; Castor, C; Bon, F; Megraud, F; Le Cann, Pierre; Desenclos, Jc.
A large waterborne outbreak of infection that occurred during August 2000 in a local community in France was investigated initially via a rapid survey of visits to local physicians. A retrospective cohort study was then conducted on a random cluster sample of residents. Of 709 residents interviewed, 202 (28.5%) were definite cases (at least three liquid stools/day or vomiting) and 62 (8.7%) were probable cases (less than three liquid stools/day or abdominal pain). Those who had drunk tap water had a three-fold increased risk for illness (95% CI 2.4-4.0). The risk increased with the amount of water consumed (chi-square trend: p &lt; 0.0001). Bacteriological analyses of stools were performed for 35 patients and virological analyses for 24 patients....
Tipo: Text
Palavras-chave: Waterborne outbreak; Rotavirus; Norovirus; Gastroenteritis; Epidemiology; Campylobacter coli.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1696.pdf

Noroviruses are highly infectious but there is strong variation in host susceptibility and virus pathogenicity
Provedor de dados: 5
Autores: Teunis, Peter Fm; Le Guyader, Soizick; Liu, Pengbo; Ollivier, Joanna; Moe, Christine L.
Noroviruses are a major public health concern: their high infectivity and environmental persistence have been documented in several studies. Genetic sequencing shows that noroviruses are highly variable, and exhibit rapid evolution. A few human challenge studies have been performed with norovirus, leading to estimates of their infectivity. However, such incidental estimates do not provide insight into the biological variation of the virus and the interaction with its human host. To study the variation in infectivity and pathogenicity of norovirus, multiple challenge studies must be analysed jointly, to compare their differences and describe how virus infectivity and host susceptibility vary. Since challenge studies can only provide a small sample of the...
Tipo: Text
Palavras-chave: Challenge studies; Infectivity; Natural experiments; Norovirus; Multilevel dose response.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00641/75265/75474.pdf

Optimization of PMAxx pretreatment to distinguish between human norovirus with intact and altered capsids in shellfish and sewage samples
Provedor de dados: 5
Autores: Randazzo, Walter; Khezri, Mohammad; Ollivier, Joanna; Le Guyader, Soizick; Rodriguez-diaz, Jesus; Aznar, Rosa; Sanchez, Gloria.
Shellfish contamination by human noroviruses (HuNoVs) is a serious health and economic problem. Recently an ISO procedure based on RT-qPCR for the quantitative detection of HuNoVs in shellfish has been issued, but these procedures cannot discriminate between inactivated and potentially infectious viruses. The aim of the present study was to optimize a pretreatment using PMAxx to better discriminate between intact and heat-treated HuNoVs in shellfish and sewage. To this end, the optimal conditions (30 min incubation with 100 μM of PMAxx and 0.5% of Triton, and double photoactivation) were applied to mussels, oysters and cockles artificially inoculated with thermally-inactivated (99 °C for 5 min) HuNoV GI and GII. This pretreatment reduced the signal of...
Tipo: Text
Palavras-chave: Intercalating dyes; Viability PCR; Norovirus; Shellfish; Sewage; RT-qPCR.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00411/52208/52970.pdf

Passive Samplers, a Powerful Tool to Detect Viruses and Bacteria in Marine Coastal Areas
Provedor de dados: 5
Autores: Vincent Hubert, Francoise; Wacrenier, Candice; Morga, Benjamin; Lozach, Solen; Quenot, Emmanuelle; Mege, Mickael; Lecadet, Cyrielle; Gourmelon, Michele; Hervio-heath, Dominique; Le Guyader, Soizick.
The detection of viruses and bacteria which can pose a threat either to shellfish health or shellfish consumers remains difficult. The current detection methods rely on point sampling of water, a method that gives a snapshot of the microorganisms present at the time of sampling. In order to obtain better representativeness of the presence of these microorganisms over time, we have developed passive sampling using the adsorption capacities of polymer membranes. Our objectives here were to assess the feasibility of this methodology for field detection. Different types of membrane were deployed in coastal waters over 2 years and the microorganisms tested using qPCR were: human norovirus (NoV) genogroups (G)I and II, sapovirus, Vibrio spp. and the species...
Tipo: Text
Palavras-chave: Norovirus; Ostreid herpes virus 1 &amp;#956; Var; Vibrio spp; Microbial source tracking; Sea; Passive sampler; Oyster (Crassostrea gigas).
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00682/79375/81893.pdf

Improving the efficacy of sewage treatment decreases norovirus contamination in oysters
Provedor de dados: 5
Autores: Schaeffer, Julien; Treguier, Cathy; Piquet, Jean-come; Gachelin, Sonia; Cochennec-laureau, Nathalie; Le Saux, Jean-claude; Garry, Pascal; Le Guyader, Soizick.
As human population increases worldwide, water quality will become increasingly problematic, and food consumed raw may be of higher risk. This is already evident for oysters grown in coastal areas - despite regulations based on bacterial indicators, oysters are still implicated in food-borne outbreaks worldwide. The pathogens most frequently detected are human noroviruses, which are shed at high concentrations in human excreta and are very resistant to environmental conditions. Sewage treatment plants usually apply a variety of steps such as activated sludge treatment, chlorine or UV disinfection to eliminate contaminants, these processes have variable efficacy. This study demonstrates the impact of replacing an old lagoon-based sewage treatment plant with...
Tipo: Text
Palavras-chave: Norovirus; Shellfish safety; Sewage treatment; Coastal water quality.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00450/56140/57707.pdf

Scientific Opinion on Norovirus (NoV) in oysters: methods, limits and control options
Provedor de dados: 5
NoV is highly infectious, and there is no threshold infectivity limit for NoV detected by PCR. The probability of becoming infected increases with the dose but depends also on the characteristics of the organism, the food matrix and the host factors. The relationship between the number of infectious virus particles and the number of virus genome copies detected by quantitative PCR is not a constant, and it is important to realise that the infectious risk associated with low level positive oysters as determined by real-time PCR may be overestimated. Quantitative data on viral load from areas compliant with current EU legislative requirements (E. coli standards) during January-March 2010 in 3 selected member states, show that a viral limit of 100, 200, 500,...
Tipo: Text
Palavras-chave: Norovirus; Oysters; Diagnostic methods; Microbiological criteria; Treatment; Control.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00066/17735/15256.pdf

Environmental Conditions Leading to Shellfish Contamination and Related Outbreaks
Provedor de dados: 5
Autores: Maalouf, Haifa; Pommepuy, Monique; Le Guyader, Soizick.
Human fecal wastes contain a large variety of viruses that can enter the environment through discharge of waste materials from infected individuals. Despite the high diversity of viruses that are introduced into the environment by human fecal pollution, only a few have been recognized to cause disease in association with consumption of contaminated shellfish. To explain bivalve mollusks contamination, several factors including human epidemiology, virus persistence through sewage treatment plant, and shellfish uptake may be suggested. Considering different outbreaks described in the literature, the most common route for transmission is accidental contamination after heavy rainfall, when extra loads cause an overflow, and release of untreated sewage into the...
Tipo: Text
Palavras-chave: Shellfish; Outbreaks; Norovirus; Environmental conditions.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00011/12252/9031.pdf

Qualité microbiologique des zones conchylicoles dans les Pertuis Charentais
Provedor de dados: 5
Autores: Thomas, Gerard; Piquet, Jean-come; Ryckaert, Mireille; Masson, Daniel; Pepin, Jean-francois; Polsenaere, Pierre; Fillon, Alain.
Marine environment contamination can affect fishing and shellfish farming products (and shellfish in the first place), thus making them improper for human consumption, threatening public health and producer’s business. Analysis and health risk management are mandatory for consumer’s security and shellfish production survival.  About microbial risk, monitoring shellfish production areas and several studies carried by LERPC laboratory give a good view of the microbial contamination impacting the Pertuis Charentais shellfish production area. On the other hand, State-Region Plan Contract (CPER) studies results from 2007 to 2013 are presented and discussed; additionally, the law-prescribed sanitary studies for professional on foot-fishing classification are...
Tipo: Text
Palavras-chave: Microbiologie; E. coli; Norovirus; Étude sanitaire; Conchyliculture; Pêche à pied professionnelle; Pertuis Charentais; Microbiology; E.coli; Norovirus; Sanitary(health) studies; Shellfish farming; On foot]fishing; Pertuis Charentais.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00302/41299/40472.pdf

Rapport d’activités 2018 – Laboratoire Santé Environnement et Microbiologie.
Provedor de dados: 5
Autores: Desdouits, Marion; Garry, Pascal; Gourmelon, Michele; Hervio Heath, Dominique; Hubert, Francoise; Kaelin, Gaelle; Kergaravat, Cedric; Le Mennec, Cecile; Le Guyader, Soizick; Loiseau, Veronique; Lozach, Solen; Maillot, Jessica; Ollivier, Joanna; Parnaudeau, Sylvain; Piquet, Jean-come; Quenot, Emmanuelle; Rocq, Sophie; Schaeffer, Julien; Serghine, Joelle; Vallade, Emilie; Veron, Antoine; Wacrenier, Candice.
Ce rapport présente une synthèse des actions, et travaux réalisés par le laboratoire Santé Environnement et Microbiologie pendant l’année 2018. Les divers projets de recherche sont résumés et les derniers développements ou résultats sont brièvement évoqués. Les actions en tant que Laboratoire National de Référence pour la Microbiologie des Coquillages et les activités pour la coordination du réseau REMI sont également présentées.
Tipo: Text
Palavras-chave: Microbiologie sanitaire; Bactéries entériques; Vibrions; Virus entériques humains; Norovirus; Coquillage; Activité de référence; REMI.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00491/60218/63584.pdf

Adsorption of norovirus and ostreid herpesvirus type 1 to polymer membranes for the development of passive samplers
Provedor de dados: 5
Autores: Hubert, Francoise; Morga, Benjamin; Renault, Tristan; Le Guyader, Soizick.
Aims  This study was performed to develop passive sampling methodology for the detection of two viruses in seawater in the area of shellfish production, The Norovirus (NoV), a human pathogen implicated in gastroenteritis outbreaks linked to oyster consumption and the ostreid herpesvirus type 1 (OsHV-1) a virus associated with mass mortalities of Pacific oysters. Methods and Results  Commercially membranes were tested for their capacity to adsorb virus: Zetapor, gauze, nylon, low density polyethylene (LDPE) and Polyvinylidene difluoride (PVDF). Laboratory exposures of membranes to contaminated water samples (stool, sewage, seawater) were performed. Our data shown that the amount of NoV GII genome per membrane measured with qRT-PCR increased with the time of...
Tipo: Text
Palavras-chave: Norovirus; OsHV-1; Pacific oysters; Passive samplers; Seawater; Sewage.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00365/47646/47670.pdf

Virus entériques humains et coquillages
Provedor de dados: 5
Autores: Parnaudeau, Sylvain; Le Mennec, Cecile; Miura, Takayuki; Le Saux, Jean-claude; Le Guyader, Soizick.
Human enteric viruses discharged in raw sewage are hightly resistant and may be detected in surface waters, coastal areas and shellfish. Whereas noroviruses, responsible for relatively benign acute gastroentritis, are the most common agents implicated in shellfish borne outbreaks, other human viruses which cause severe human diseases, such as hepatitis A virus, may also be transmitted. Environmental studies have demonstrated that virus concentrations detected in shellfish are higher than in surrounding waters. This concentration may explain the high prevalence of noroviruses detected in various shellfish species collected in different countries. If many questions remain regarding viral contamination of shellfish, the most important seems to be the...
Tipo: Text
Palavras-chave: Shellfish; Human enteric viruses; Norovirus; Hepatitis A virus; Coquillage; Virus entérique humain; Norovirus; Virus de l'hépatite A.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00317/42849/42258.pdf

Viral acute gastroenteritis: clinical and epidemiological features of co-infected patients
Provedor de dados: 55
Autores: Ferreira,Carla Elizabeth de Oliveira; Raboni,Sonia Mara; Pereira,Luciane Aparecida; Nogueira,Meri Bordignon; Vidal,Luine Rosele Renaud; Almeida,Sérgio Monteiro.
BACKGROUND: Acute gastroenteritis (AGE) is a common disorder that affects children worldwide. It is usually caused by viral agents, including rotavirus, enteric adenovirus, norovirus, and astrovirus groups. Currently, there are few reports about co-infection among these viruses, mainly in Brazil. METHODS: This is a retrospective study in which 84 rotavirus-positive samples from hospitalized patients at a teaching hospital in Southern Brazil, collected in the 2001-2010 period, were analyzed by polymerase chain reaction (PCR) and reverse transcription - polymerase chain reaction (RT-PCR), for the investigation of enteric adenovirus, astrovirus, and norovirus. RESULTS: In total, 12 of the 84 (14%) samples were positive to enteric adenovirus or norovirus....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Co-infection; Viral gastroenteritis; Oiarrhea; Rotavirus A; Norovirus; Enteric adenovirus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000300009

Evaluation of skimmed milk flocculation method for virus recovery from tomatoes
Provedor de dados: 58
Autores: Melgaço,Fabiana Gil; Corrêa,Adriana Abreu; Ganime,Ana Carolina; Brandão,Marcelo Luiz Lima; Medeiros,Valéria de Mello; Rosas,Carla de Oliveira; Lopes,Silvia Maria dos Reis; Miagostovich,Marize Pereira.
Abstract This study aimed to evaluate the elution-concentration methodology based on skimmed milk flocculation from three varieties of tomatoes (Solanum lycopersicum L. [globe], Solanum lycopersicum var. cerasiforme [cherry] and hybrid cocktail [grape tomato]) for further monitoring of field samples. Spiking experiments were performed to determine the success rate and efficiency recovery of human norovirus (NoV) genogroup II, norovirus murine-1 (MNV-1) used as sample process control virus and human adenovirus (HAdV). Mean values of 18.8%, 2.8% and 44.0% were observed for NoV GII, MNV-1 and HAdV, respectively with differences according to the types of tomatoes, with lower efficiency for cherry tomatoes. Analysis of 90 samples, obtained at commercial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adenovirus; Flocculation; Murine norovirus-1; Norovirus; Tomato.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500034

Identification and Molecular Characterization of Norovirus in São Paulo State, Brazil
Provedor de dados: 58
Autores: Morillo,Simone Guadagnucci; Audrey,Cilli; Carmona,Rita de Cássia Compagnoli; Timenetsky,Maria do Carmo Sampaio Tavares.
Norovirus (NoV), previously called Norwalk-like virus, represents an important group of human pathogens associated with outbreaks of nonbacterial gastroenteritis. Epidemiological surveys of outbreaks have shown that the most important routes of transmission are person-to-person contacts and contaminated food and water, with the virus affecting adults and children. NoV is classified into genogroups, being genogroups GI, GII and GIV found in humans. In view of the high genetic diversity of NoV and the lack of information about this virus in Brazil, the aim of the present study was the molecular characterization of NoV isolated from diarrheic stool samples of patients from São Paulo State. In this study, 204 stool specimens collected during diarrhea outbreaks...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Norovirus; Genogroup GII; Molecular characterization.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400004

Caliciviruses in hospitalized children, São Luís, Maranhão, 1997-1999: detection of norovirus GII.12
Provedor de dados: 58
Autores: Portal,Thayara Morais; Siqueira,Jones Anderson Monteiro; Costa,Larissa Cristina Prado das Neves; Lima,Ian Carlos Gomes de; Lucena,Maria Silvia Sousa de; Bandeira,Renato da Silva; Linhares,Alexandre da Costa; Luz,Claudia Regina Nunes Eloi da; Gabbay,Yvone Benchimol; Resque,Hugo Reis.
ABSTRACT Gastroenteritis is one of the most common diseases during childhood, with norovirus (NoV) and sapovirus (SaV) being two of its main causes. This study reports for the first time the incidence of these viruses in hospitalized children with and without gastroenteritis in São Luís, Maranhão. A total of 136 fecal samples were tested by enzyme immunoassays (EIA) for the detection of NoV and by reverse transcription-polymerase chain reaction (RT-PCR) for detection of both NoV and SaV. Positive samples for both agents were subjected to sequencing. The overall frequency of NoV as detected by EIA and RT-PCR was 17.6% (24/136) and 32.6% (15/46), respectively in diarrheic patients and 10.0% (9/90) in non-diarrheic patients (p &lt; 0.01). Of the diarrheic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Norovirus; Sapovirus; Gastroenteritis; Children.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300724

Molecular screening of blue mussels indicated high mid-summer prevalence of human genogroup II Noroviruses, including the pandemic "GII.4 2012" variants in UK coastal waters during 2013
Provedor de dados: 58
Autores: Biswas,Subhajit; Jackson,Philippa; Shannon,Rebecca; Dulwich,Katherine; Sukla,Soumi; Dixon,Ronald A.
Abstract This molecular study is the first report, to the best of our knowledge, on identification of norovirus, NoV GII.4 Sydney 2012 variants, from blue mussels collected from UK coastal waters. Blue mussels (three pooled samples from twelve mussels) collected during the 2013 summer months from UK coastal sites were screened by RT-PCR assays. PCR products of RdRP gene for noroviruses were purified, sequenced and subjected to phylogenetic analysis. All the samples tested positive for NoVs. Sequencing revealed that the NoV partial RdRP gene sequences from two pooled samples clustered with the pandemic "GII.4 Sydney variants" whilst the other pooled sample clustered with the NoV GII.2 variants. This molecular study indicated mussel contamination with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blue mussels; Norovirus; GII.4 Sydney variant; Coastal water.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200279