Consulta: subjectFacets:"Oxidative stress"
Registros recuperados: 283
Data/hora: 28/05/2024 17:43:57

Respuestas morfológicas y fisiológicas de Rhizophagus intraradices y Medicago sativa ante contaminación por diésel.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Hernández Ortega, Herminia Alejandra.
Este estudio evaluó el crecimiento, cambios morfológicos, actividades fisiológicas y expresión de genes en Medicago sativa en respuesta al estrés por la contaminación con diésel, y por la inoculación del hongo micorrízico arbuscular Rhizophagus intraradices. Este trabajo fue dividido en cuatro capítulos que describen cuatro diferentes fases experimentales. El primer capítulo describe el efecto del diésel en el desarrollo y colonización de R. intraradices. El segundo capítulo presenta resultados relacionados con el crecimiento y la expresión de genes en respuesta al estrés oxidativo generado por la contaminación con diésel en M. sativa. El tercer capítulo describe los cambios anatómicos e hidráulicos de las plantas de M. sativa en presencia de diésel. El...
Palavras-chave: Cambios anatómicos; Estrés oxidativo; Expresión génica; Intercambio gaseoso; Anatomical changes; Oxidative stress; Gene expression; Gas exchange; Edafología; Doctorado.
Ano: 2014
URL: http://hdl.handle.net/10521/2265

Responses of plankton and fish from temperate zones to UVR and temperature in a context of global change
Provedor de dados: Ecología Austral
Autores: Gonçalves,Rodrigo J; Souza,María Sol; Aigo,Juana; Modenutti,Beatriz; Balseiro,Esteban; Villafañe,Virginia E; Cussac,Víctor; Helbling,E Walter.
In the last decades, both temperature and UVB (280-315 nm) radiation on the surface of the Earth increased at growing rates as a result of human activities. Many studies had evaluated the effects of temperature on aquatic ecosystems, but now the field broadens as the combination and variations of temperature and radiation gains especial importance. In this work we attempt to revisit some of our knowledge about the effects of UVR and temperature on marine and freshwater plankton and fish from temperate regions (defined here as latitudes between 30-60º), especially from the Patagonia area, due to the special characteristics found in these sites. UVR affects (often negatively) almost all processes, from carbon fixation to behavior; and certainly all trophic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Climate change; Bacterioplankton; Phytoplankton; Zooplankton; Fish; Oxidative stress; Upper mixed layer.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1667-782X2010000200005

Effect of the Puyehue-Cordon Caulle volcanic complex eruption on crustacean zooplankton of Andean lakes
Provedor de dados: Ecología Austral
Autores: Balseiro,Esteban; Souza,María S.; Serra Olabuenaga,Ignacio; Wolinski,Laura; Bastidas Navarro,Marcela; Laspoumaderes,Cecilia; Modenutti,Beatriz.
We studied the effect of volcanic ash on the crustacean zooplankton (mainly cladocerans) in the Andean North-Patagonian lakes that were affected by the eruption of the Puyehue-Cordón Caulle complex. The study was carried out at three different scales: sub cellular, population and ecosystemic. Cladocerans are filter feeding organisms that cannot distinguish between food (algae less than 30 µm) and suspended ash, therefore when feeding they ingest this material. The ash affected negatively the individuals at a biochemical level, increasing the oxidative stress reacting enzymes like Glutation-S-transferase and the biomarker Acetyl cholinesterase. At the population level, we recorded a decrease in survival and fecundity, in particular, when ash concentration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Volcanic ash; Cladocerans; Oxidative stress; Survivorship; Trophic level.
Ano: 2014
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1667-782X2014000100009

Discrimination of population of recombinant inbred lines of rye (Secale cereale L.) for different responses to nitrogen-potassium stress assessed at the seedling stage under in vitro conditions
Provedor de dados: Electronic Journal of Biotechnology
Autores: Smolik,Mi&amp;#322;osz.
Background: Plants differ in the methods used to acquire nutrients from environments with low nutrient availability, and may change the morphology of their ‘root architecture’ to be able to take up nutrients. Results: In the present study rye response to stress caused by high and low nitrogen-potassium treatments in mature embryos cultures was described within a population consisting of one hundred and thirty eight recombinant inbred lines of rye. Characterization of the response of recombinant inbred lines (RILs) to nutrient stress was presented as the results of analyses of morphological traits, and physiological and biochemical parameters of the seedlings grown in both treatments. A wide range of variability of individual RILs to induced stress was...
Tipo: Journal article
Palavras-chave: Mature embryos; Nutrient stress; Oxidative stress; RILs; Rye.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000100005

Oxidative stress assessment by glutathione peroxidase activity and glutathione levels in response to selenium supplementation in patients with Mucopolysaccharidosis I, II and VI
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira-Silva,José Araújo de; Yamamoto,Joyce Umbelino Pinto; Oliveira,Renata Bernardes de; Monteiro,Vaneisse Cristina Lima; Frangipani,Beatriz Jurkiewcz; Kyosen,Sandra Obikawa; Martins,Ana Maria; D’Almeida,Vânia.
Abstract We assessed levels of plasma selenium (Se), selenoproteins and their change after Se supplementation in patients with mucopolysaccharidosis (MPS) types I, II and VI. This was done in a retrospective study of the medical records of 30 patients with MPS I (n=13), MPS II (n=9) and MPS VI (n=8) who were being treated with enzyme replacement therapy. As part of routine nutritional monitoring, Se levels were measured, revealing that 28 patients (93.3%) had values below the normal range. Therefore, they received supplementation for 12 months, and Se was measured after 6 and 12 months. Glutathione peroxidase (GPx) activity, total glutathione (GSHt), oxidized glutathione (GSSG) and reduced glutathione (GSH) were measured at baseline and 6 months after Se...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucopolysaccharidosis; Oxidative stress; Selenium; Glutathione peroxidase.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100001

DNA repair diseases: what do they tell us about cancer and aging?
Provedor de dados: Genetics and Molecular Biology
Autores: Menck,Carlos FM; Munford,Veridiana.
The discovery of DNA repair defects in human syndromes, initially in xeroderma pigmentosum (XP) but later in many others, led to striking observations on the association of molecular defects and patients' clinical phenotypes. For example, patients with syndromes resulting from defective nucleotide excision repair (NER) or translesion synthesis (TLS) present high levels of skin cancer in areas exposed to sunlight. However, some defects in NER also lead to more severe symptoms, such as developmental and neurological impairment and signs of premature aging. Skin cancer in XP patients is clearly associated with increased mutagenesis and genomic instability, reflecting the defective repair of DNA lesions. By analogy, more severe symptoms observed in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xeroderma pigmentosum; Ultraviolet; DNA damage; Oxidative stress; DNA repair.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200008

GSTT1 and GSTM1 null variants in Mestizo and Amerindian populations from northwestern Mexico and a literature review
Provedor de dados: Genetics and Molecular Biology
Autores: Palma-Cano,Luz Elena; Córdova,Emilio J.; Orozco,Lorena; Martínez-Hernández,Angélica; Cid,Miguel; Leal-Berumen,Irene; Licón-Trillo,Angel; Lechuga-Valles,Ruth; González-Ponce,Mauricio; González-Rodríguez,Everardo; Moreno-Brito,Verónica.
Abstract The GSTT1 and GSTM1 genes are key molecules in cellular detoxification. Null variants in these genes are associated with increase susceptibility to developing different types of cancers. The aim of this study was to determine the prevalence of GSTT1 and GSTM1 null genotypes in Mestizo and Amerindian individuals from the Northwestern region of Mexico, and to compare them with those reported worldwide. GSTT1 and GSTM1 null variants were genotyped by multiplex PCR in 211 Mestizos and 211 Amerindian individuals. Studies reporting on frequency of GSTT1 and GSTM1 null variants worldwide were identified by a PubMed search and their geographic distribution were analyzed. We found no significant differences in the frequency of the null genotype for GSTT1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; GSTT1; GSTM1; Null variants.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500727

An association study of FOXO3 variant and longevity
Provedor de dados: Genetics and Molecular Biology
Autores: Silva-Sena,Geralda Gillian; Camporez,Daniela; Santos,Lígia Ramos dos; Silva,Aline Sesana da; Sagrillo Pimassoni,Lúcia Helena; Tieppo,Alessandra; Pimentel Batitucci,Maria do Carmo; Morelato,Renato Lírio; Paula,Flavia de.
Abstract Human longevity is a polygenic and multifactorial trait. Pathways related to lifespan are complex and involve molecular, cellular, and environmental processes. In this analytical observational study, we evaluated the relationship between environment factors, oxidative stress status, DNA integrity level, and the association of FOXO3 (rs2802292), SOD2 (rs4880), APOE (rs429358 and rs7412), and SIRT1 (rs2273773) polymorphisms with longevity in oldest-old individuals from southeastern Brazil. We found an association between the FOXO3 GG genotype and gender. While lifestyle, anthropometric, and biochemical characteristics showed significant results, DNA damage and oxidative stress were not related to lifespan. We found that long-lived individuals with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lifespan; SNPs; Environmental factors; Oxidative stress; Genomic damage.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300386

Adaptive response: some underlying mechanisms and open questions
Provedor de dados: Genetics and Molecular Biology
Autores: Dimova,Evgeniya G.; Bryant,Peter E.; Chankova,Stephka G.
Organisms are affected by different DNA damaging agents naturally present in the environment or released as a result of human activity. Many defense mechanisms have evolved in organisms to minimize genotoxic damage. One of them is induced radioresistance or adaptive response. The adaptive response could be considered as a nonspecific phenomenon in which exposure to minimal stress could result in increased resistance to higher levels of the same or to other types of stress some hours later. A better understanding of the molecular mechanism underlying the adaptive response may lead to an improvement of cancer treatment, risk assessment and risk management strategies, radiation protection, e.g. of astronauts during long-term space flights. In this mini-review...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adaptive response; Oxidative stress; DNA repair up-regulation; DNA-binding proteins; Antioxidant defense system.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300002

Combined GSTM1 and GSTT1 null genotypes are strong risk factors for atherogenesis in a Serbian population
Provedor de dados: Genetics and Molecular Biology
Autores: Grubisa,Ivana; Otasevic,Petar; Vucinic,Nada; Milicic,Biljana; Jozic,Tanja; Krstic,Slobodan; Milasin,Jelena.
Abstract Oxidative stress (OS) plays an important role in atherogenesis and since glutathione S-transferases (GSTs) provide protection against OS, we have tested the hypothesis that deletion polymorphisms in two GSTs (GSTM1 and GSTT1) may affect the risk of developing atherosclerosis. A total of 382 individuals (200 patients with atherosclerosis and 182 healthy controls) were included in this association study. Genomic DNA was isolated from peripheral blood cells or from buccal epithelial cells and genotyping was performed using multiplex-PCR or real-time PCR methods. GSTM1 null genotype was significantly more frequent in atherosclerotic patients than in controls (52.0% vs 34.1%) and individuals with the GSTM1 null genotype had an approximately 2-fold...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Gene polymorphisms; Glutathione S-transferases; Oxidative stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100035

Differential gene expression between wild-type and Gulo-deficient mice supplied with vitamin C
Provedor de dados: Genetics and Molecular Biology
Autores: Jiao,Yan; Zhang,Jifei; Yan,Jian; Stuart,John; Gibson,Griffin; Lu,Lu; Willaims,Robert; Wang,Yong Jun; Gu,Weikuan.
The aim of this study was to test the hypothesis that hepatic vitamin C (VC) levels in VC deficient mice rescued with high doses of VC supplements still do not reach the optimal levels present in wild-type mice. For this, we used a mouse scurvy model (sfx) in which the L-gulonolactone oxidase gene (Gulo) is deleted. Six age- (6 weeks old) and gender- (female) matched wild-type (WT) and sfx mice (rescued by administering 500 mg of VC/L) were used as the control (WT) and treatment (MT) groups (n = 3 for each group), respectively. Total hepatic RNA was used in triplicate microarray assays for each group. EDGE software was used to identify differentially expressed genes and transcriptomic analysis was used to assess the potential genetic regulation of Gulo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene expression; L-gulonolactone oxidase; Liver; Oxidative stress; Vitamin C.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300004

Evaluation of oxidative stress markers and cardiovascular risk factors in Fabry Disease patients
Provedor de dados: Genetics and Molecular Biology
Autores: Müller,Karen B.; Galdieri,Luciano C.; Pereira,Vanessa G.; Martins,Ana M.; D'Almeida,Vânia.
Fabry Disease, an X-linked inborn error of metabolism, is characterized by progressive renal insufficiency, with cardio and cerebrovascular involvement. Homocysteine (Hcy) is considered a risk factor for vascular diseases, but the mechanisms by which it produces cardiovascular damage are still poorly understood. Regarding the vascular involvement in FD patients, the analysis of factors related to thromboembolic events could be useful to improving our understanding of the disease. The aim of this study was to evaluate plasma Hcy and other parameters involved in the methionine cycle, as well as oxidative stress markers. The sample consisted of a group of 10 male FD patients and a control group of 8 healthy individuals, paired by age. Venous blood was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inborn errors of metabolism; Fabry Disease; Homocysteine; Oxidative stress.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300006

Активность ферментов пероксидного комплекса тканей мидии Mytilus galloprovincialis Lam в норме и в условиях естественного окислительного стресса
Provedor de dados: IBSS Repository
Autores: Гостюхина, О. Л.; Солдатов, А. А.; Головина, И. В.
Исследовано содержание продуктов перекисного окисления липидов (ПОЛ) и состояние ферментативного пероксидного комплекса (ПК) (глутатионпероксидазы (ГП), каталазы и пероксидазы) в гепатопанкреасе, жабрах и ноге черноморского моллюска Mytilus galloprovincialis Lam. в условиях физиологического покоя и нереста. Последнее рассматривали как модель естественного окислительного стресса. Изучали особей двух цветовых морф - черной и коричневой. Выявлена тканевая специфика активностей ферментов и распределения продуктов ПОЛ в состоянии нормы, а также особенности ответных реакций тканей моллюсков обеих морф при действии окислительной нагрузки. Наиболее чувствительной к действию перекисных продуктов в условиях естественного окислительного стресса оказались особи черной...
Tipo: Journal Contribution
Palavras-chave: Ферменты; Каталаза; Пероксидаза; Глутатионпероксидаза; Перекисное окисление липидов; Окислительный стресс; Мидии; Цветовые морфы; Нерест; Enzymes; Catalase; Peroxidase; Glutathione peroxidase; Lipid peroxidation; Spawning; Oxidative stress; Mussels; Color morphs Spawning Enzymes Lipids Spawning Enzymes Lipids http://aims.fao.org/aos/agrovoc/c_14490 http://aims.fao.org/aos/agrovoc/c_2603 http://aims.fao.org/aos/agrovoc/c_4362.
Ano: 2005
URL: http://repository.ibss.org.ua/dspace/handle/99011/226

Parental exposure to the herbicide diuron results in oxidative DNA damage to germinal cells of the Pacific oyster Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Barranger, Audrey; Heude-berthelin, Clothilde; Rouxel, Julien; Adeline, Beatrice; Benabdelmouna, Abdellah; Burgeot, Thierry; Akcha, Farida.
Chemical pollution by pesticides has been identified as a possible contributing factor to the massive mortality outbreaks observed in Crassostrea gigas for several years. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to the herbicide diuron at environmental concentrations during gametogenesis. This trans-generational effect occurs through damage to genitor-exposed gametes, as measured by the comet-assay. The presence of DNA damage in gametes could be linked to the formation of DNA damage in other germ cells. In order to explore this question, the levels and cell distribution of the oxidized base lesion 8-oxodGuo were studied in the gonads of exposed genitors. High-performance liquid chromatography...
Tipo: Text
Palavras-chave: Crassostrea gigas; Oxidative stress; DNA damage; Germ cell; Pesticides.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00295/40655/39753.pdf

Cross talk: Two way allelopathic interactions between toxic Microcystis and Daphnia
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Bojadzija Savic, Gorenka; Bormans, Myriam; Edwards, Christine; Lawton, Linda; Briand, Enora; Wiegand, Claudia.
Due to eutrophication, freshwater ecosystems frequently experience cyanobacterial blooms, many of which produce bioactive metabolites that can affect vertebrates and invertebrates life traits. Zooplankton are able to develop tolerance as a physiological response to cyanobacteria and their bioactive compounds, however, this comes with energetic cost that in turn influence Daphnia life traits and may impair populations. Vice versa, it has been suggested that Daphnia are able to reduce cyanobacterial dominance until a certain cyanobacterial density; it remains unclear whether Daphnia metabolites alone influence the physiological state and bioactive metabolites production of cyanobacteria. Hence, this study investigates mutual physiological reactions of toxic...
Tipo: Text
Palavras-chave: Zooplankton; Cyanobacteria; Secondary metabolites; PCC7806; Toxic; Oxidative stress.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00624/73653/76105.pdf

Comparative embryotoxicity and genotoxicity of the herbicide diuron and its metabolites in early life stages of Crassostrea gigas: implication of reactive oxygen species production
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Behrens, Daphne; Rouxel, Julien; Burgeot, Thierry; Akcha, Farida.
Herbicides are one of the major classes of pollutants contaminating coastal waters over the world. Among them, diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea) is a phenylurea herbicide frequently detected in oyster-producing area, known to be toxic for this important exploited non-target species. With the aim to investigate the mechanisms by which diuron displays its toxicity in oyster, the implication of both biotransformation and oxygen reactive species (ROS) production was studied considering embryotoxicity and genotoxicity as endpoints. Comparative embryotoxicity and genotoxicity of diuron and its main metabolites (DCPMU, DCPU and 3,4-DCA) were thus studied on oyster larvae by the embryo-larval bioassay on D larvae and the comet assay on trochophore...
Tipo: Text
Palavras-chave: Oyster; Herbicide; Embryotoxicity; Genotoxicity; Oxidative stress; Biotransformation.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00324/43521/43053.pdf

Effect of dietary probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress status of shrimp Litopenaeus stylirostris
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Castex, Mathieu; Lemaire, Pierrette; Wabete, Nelly; Chim, Liet.
in this study we evaluated the effects of the probiotic Pediococcus acidilactici MA18/5M on shrimp, Litopenaeus stylirostris (also called Penaeus stylirostris), first on antioxidant defences and secondly on the oxidative stress status in the shrimps' haemolymph and digestive gland. We conducted two experiments with the same protocol in which shrimps were fed two diets for three weeks: a control diet and a probiotic diet containing 1 g of live P. acidilactici MA18/5M kg(-1). In the first experiment, the shrimps were found to be healthy over the trial period; no mortalities and non-detectable signs of infection were recorded. These resulted in high final survival rates (above 90% in both treatments). On the other hand, during the second trial, carried out at...
Tipo: Text
Palavras-chave: Vibriosis; Antioxidant defences; Oxidative stress; Probiotic; Shrimps.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6780.pdf

Variation in the link between oxygen consumption and ATP production, and its relevance for animal performance
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Salin, Karine; Auer, Sonya K.; Rey, Benjamin; Selman, Colin; Metcalfe, Neil B.
It is often assumed that an animal's metabolic rate can be estimated through measuring the whole-organism oxygen consumption rate. However, oxygen consumption alone is unlikely to be a sufficient marker of energy metabolism in many situations. This is due to the inherent variability in the link between oxidation and phosphorylation; that is, the amount of adenosine triphosphate (ATP) generated per molecule of oxygen consumed by mitochondria (P/O ratio). In this article, we describe how the P/O ratio can vary within and among individuals, and in response to a number of environmental parameters, including diet and temperature. As the P/O ratio affects the efficiency of cellular energy production, its variability may have significant consequences for animal...
Tipo: Text
Palavras-chave: Mitochondrial coupling efficiency; Life history; Oxidative stress; Reactive oxygen species; Trade-off; Uncoupling.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00414/52509/53322.pdf

Influence de l'environnement trophique de l'élevage en biofloc sur les performances physiologiques de la crevette Litopenaeus stylirostris : Étude de paramètres de la nutrition, de l'immunité et de la reproduction
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Cardona, Emilie.
Biofloc is an intensive rearing system with zero or minimal water exchange where a diverse population of microorganisms (microalgae, zooplankton and bacteria) develops in association with organic matter to form the floc particles. These particles play the double role of biological filter and dietary supplement. This dissertation aims to better understand the process of this rearing system and its interactions with the Litopenaeus stylirostris shrimp. Two specific objectives were integrated within the framework of this general objective: (i) to measure the production gain from biofloc rearing and (ii) to study the interaction between biofloc environment and shrimp and to assess its role on production performances of shrimps. Thus, our results show...
Tipo: Text
Palavras-chave: Biofloc; Crevette; Nutrition; Stress oxydant; Défenses antioxydantes; Immunité; Reproduction; Oeufs; Larves; Bactéries; Microbiote; Biofloc; Shrimp; Nutrition; Oxidative stress; Antioxidant defences; Immunity; Reproduction; Eggs; Larvae; Bacteria; Microbiota.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00272/38294/36492.pdf

Microarray-Based Identification of Gonad Transcripts Differentially Expressed Between Lines of Pacific Oyster Selected to Be Resistant or Susceptible to Summer Mortality
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Fleury, Elodie; Moal, Jeanne; Boulo, Viviane; Daniel, Jean-yves; Mazurais, David; Henaut, Alain; Corporeau, Charlotte; Boudry, Pierre; Favrel, Pascal; Huvet, Arnaud.
Summer mortality of the Pacific oyster Crassostrea gigas is the result of a complex interaction between oysters, their environment, and pathogens. Heredity appears to be a major factor determining the sensitivity of oysters to summer mortality, allowing resistant (R) and susceptible (S) lines to be produced. We conducted genome-wide expression profiling of R and S gonads during the 3-month period preceding a summer mortality event, using a cDNA microarray that we designed. ANOVA analysis revealed that 34 genes were differentially expressed between R and S lines on four dates preceding the mortality event. Annotation of some of these genes highlights reproduction and its allocation and antioxidant defenses as the main pathways that operate differentially...
Tipo: Text
Palavras-chave: Mollusca; Bivalvia; Gene expression; CDNA microarrays; Reproduction; Oxidative stress; Energy metabolism.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00005/11590/8258.pdf

Utilisation de bactéries lactiques probiotiques pour prémunir les poissons d'élevage contre des vibrions pathogènes
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lamari, Faouzi.
Diseases cause major production losses in fish farms. Bacteria of the genus Vibrio are known as opportunistic pathogens, and have been associated with mass-mortality episodes. To cope with these disease outbreaks, fish farmers often resort to the use of antibiotics. However, preventive overuse has led to the emergence of resistant strains and frequent treatment failures. Probiotic lactic acid bacteria are increasingly used to improve the quality of seed production in aquaculture, while limiting antibiotic treatments. Microbial strains must fulfil several criteria to be evaluated as potential probiotics.In a first step, we selected and characterized 55 strains of lactic acid bacteria isolated from fish hatchery. The first selection criterion focused on the...
Tipo: Text
Palavras-chave: Probiotiques; Microflore intestinale; Réponse immunitaire; Stress oxydatif; Malformations vertébrales; Ossification; Oxidative stress; Vertebral malformations; Dicentrarchus labrax; Artemia; Vibrio; Probiotic; Intestinal microflora; Immune response.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00498/60943/64336.pdf

Effect of probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress of Litopenaeus stylirostris under Vibrio nigripulchritudo challenge
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Castex, Mathieu; Lemaire, Pierrette; Wabete, Nelly; Chim, Liet.
Antioxidant defences and induced oxidative stress tissue damage of the blue shrimp Litopenaeus stylirostris, under challenge with Vibrio nigriputchritudo, were investigated for a 72-h period. For this purpose, L stylirostris were first infected by immersion with pathogenic V. nigripulchritudo strain SFn1 and then antioxidant defences: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (Gpx), Total antioxidant status (TAS), glutathiones and induced tissue damage (MDA and carbonyl proteins) were determined in the digestive gland at 0, 12, 24, 48 and 72 h post-infection (h.p.i.). In the meantime, TAS was also measured in the blood. Infection level of the shrimps during the challenge was followed by determining V. nigripulchritudo prevalence...
Tipo: Text
Palavras-chave: Probiotic; Pediococcus acidilactici; Shrimp; Oxidative stress; Antioxidant status; Vibrio; Immersion challenge.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00003/11419/7984.pdf

Metal bioaccumulation and physiological condition of the Pacific oyster (Crassostrea gigas) reared in two shellfish basins and a marina in Normandy (northwest France)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Seguin, A.; Caplat, C.; Serpentini, A.; Lebel, J. M.; Menet-nedelec, Florence; Costil, K.
A 5-month experiment combining a geochemical survey of metals with a bioaccumulation study in batches of Crassostrea gigas was conducted in two shellfish farming areas and a marina in Normandy (France). Various endpoints at different levels of biological organization were studied. ROCCH data showed differences in biota contamination between the two shellfish areas but the present study revealed only slight differences in metallic contamination and biomarkers. By contrast, significantly different values were recorded in the marina in comparison with the two other sites. Indeed, higher levels of Cd, Cu and Zn were measured in the oysters from the marina, and these oysters also showed a poorer physiological condition (e.g., condition index, histopathological...
Tipo: Text
Palavras-chave: Crassostrea gigas; Metallic contamination; Bioaccumulation; Physiological condition; Oxidative stress; France.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00319/43053/42632.pdf

Physiological responses of Manila clams Venerupis (=Ruditapes) philippinarum with varying parasite Perkinsus olseni burden to toxic algal Alexandrium ostenfeldii exposure
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lassudrie, Malwenn; Soudant, Philippe; Richard, Gaelle; Henry, N.; Medhioub, Walid; Da Silva, P.m.; Donval, Anne; Bunel, M.; Le Goïc, Nelly; Lambert, Christophe; De Montaudoin, X.; Fabioux, Caroline; Hegaret, Helene.
Manila clam stock from Arcachon Bay, France, is declining, as is commercial harvest. To understand the role of environmental biotic interactions in this decrease, effects of a toxic dinoflagellate, Alexandrium ostenfeldii, which blooms regularly in Arcachon bay, and the interaction with perkinsosis on clam physiology were investigated. Manila clams from Arcachon Bay, with variable natural levels of perkinsosis, were exposed for seven days to a mix of the nutritious microalga T-Iso and the toxic dinoflagellate A. ostenfeldii, a producer of spirolides, followed by seven days of depuration fed only T-Iso. Following sacrifice and quantification of protozoan parasite Perkinsus olseni burden, clams were divided into two groups according to intensity of the...
Tipo: Text
Palavras-chave: Bivalve; Harmful algal bloom; Oxidative stress; Venerupis philippinarum; Alexandrium ostenfeldii; Perkinsus olseni.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00189/30022/28507.pdf

Active and passive biomonitoring suggest metabolic adaptation in blue mussels (Mytilus spp.) chronically exposed to a moderate contamination in Brest harbor (France)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lacroix, Camille; Richard, Gaelle; Seguineau, Catherine; Guyomarch, Julien; Moraga, Dario; Auffret, Michel.
Brest harbor (Bay of Brest, Brittany, France) has a severe past of anthropogenic chemical contamination, but inputs tended to decrease, indicating a reassessment of its ecotoxicological status should be carried out. Here, native and caged mussels (Mytilus spp.) were used in combination to evaluate biological effects of chronic chemical contamination in Brest harbor. Polycyclic aromatic hydrocarbon (PAH) contamination was measured in mussel tissues as a proxy of harbor and urban pollution. Biochemical biomarkers of xenobiotic biotransformation, antioxidant defenses, generation of reducing equivalents, energy metabolism and oxidative damage were studied in both gills and digestive glands of native and caged mussels. In particular, activities of...
Tipo: Text
Palavras-chave: Marine biomonitoring; Chronic pollution; Blue mussel Mytilus spp.; Caging; Oxidative stress; Energetic metabolism; Bay of Brest.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00255/36654/35795.pdf

Unlike the Escherichia coli counterpart, archaeal RNase HII cannot process ribose monophosphate abasic sites and oxidized ribonucleotides embedded in DNA
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Malfatti, Matilde Clarissa; Henneke, Ghislaine; Balachander, Sathya; Koh, Kyung Duk; Newnam, Gary; Uehara, Ryo; Crouch, Robert J.; Storici, Francesca; Tell, Gianluca.
The presence of ribonucleoside monophosphates (rNMPs) in nuclear DNA decreases genome stability. To ensure survival despite rNMP insertions, cells have evolved a complex network of DNA repair mechanisms, in which the ribonucleotide excision repair pathway, initiated by type 2 ribonuclease H (RNase HII/2), plays a major role. We recently demonstrated that eukaryotic RNase H2 cannot repair damaged, that is, ribose monophosphate abasic (both apurinic or apyrimidinic) site (rAP) or oxidized rNMP embedded in DNA. Currently, it remains unclear why RNase H2 is unable to repair these modified nucleic acids having either only a sugar moiety or an oxidized base. Here, we compared the endoribonuclease specificity of the RNase HII enzymes from the archaeon Pyrococcus...
Tipo: Text
Palavras-chave: Ribonuclease; Bacteria; Escherichia coli (E coli); Archaea; Oxidative stress; Abasic-ribose; Oxidized-ribonucleotides; Pyrococcus abyssi; Type 2 RNase H.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00506/61796/65801.pdf

Wastewater bioremediation by mangrove ecosystems impacts crab ecophysiology: in-situ caging experiment
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Theuerkauff, Dimitri; Rivera-ingraham, Georgina A.; Lambert, Sophia; Mercky, Yann; Lejeune, Mathilde; Lignot, Jehan-hervé; Sucré, Elliott.
Mangroves are tidal wetlands that are often under strong anthropogenic pressures, despite the numerous ecosystem services they provide. Pollution from urban runoffs is one such threats, yet some mangroves are used as a bioremediation tool for wastewater (WW) treatment. This practice can impact mangrove crabs, which are key engineer species of the ecosystem. Using an experimental area with controlled WW releases, this study aimed to determine from an ecological and ecotoxicological perspective, the effects of WW on the red mangrove crab Neosarmatium africanum. Burrow density and salinity levels (used as a proxy of WW dispersion) were recorded, and a 3-week caging experiment was performed. Hemolymph osmolality, gill Na+/K+-ATPase (NKA) activity and gill...
Tipo: Text
Palavras-chave: Mangrove; Bioturbation; Wastewater; Ecophysiology; Oxidative stress; Osmoregulation.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00592/70449/68559.pdf

Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Sussarellu, Rossana; Fabioux, Caroline; Le Moullac, Gilles; Fleury, Elodie; Moraga, Dario.
Marine intertidal organisms commonly face hypoxic stress during low tide emersion; moreover, eutrophic conditions and sediment nearness could lead to hypoxic phenomena; it is indeed important to understand the molecular processes involved in the response to hypoxia. In this study the molecular response of the Pacific oyster Crassostrea gigas to prolonged hypoxia (2 mg O-2 L-1 for 20 d) was investigated under experimental conditions. A transcriptomic approach was employed using a cDNA microarray of 9058 C. gigas clones to highlight the genetic expression patterns of the Pacific oyster under hypoxic conditions. Lines of oysters resistant (R) and susceptible (S) to summer mortality were used in this study. ANOVA analysis was used to identify the genes...
Tipo: Text
Palavras-chave: Mollusca; Hypoxia; CDNA microarray; Gene expression; Oxidative stress; Respiratory chain.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00027/13799/11053.pdf

Tissue-Specific Biomarker Responses in the Blue Mussel Mytilus spp. Exposed to a Mixture of Microplastics at Environmentally Relevant Concentrations
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Revel, Messika; Lagarde, Fabienne; Perrein-ettajani, Hanane; Bruneau, Mélanie; Akcha, Farida; Sussarellu, Rossana; Rouxel, Julien; Costil, Katherine; Decottignies, Priscilla; Cognie, Bruno; Châtel, Amélie; Mouneyrac, Catherine.
The impact of a microplastic (MP) mixture composed of polyethylene (PE) and polypropylene (PP) plastic particles, prepared from commercially available products, was evaluated in blue mussels Mytilus spp. exposed to three environmentally relevant concentrations: 0.008 μg L−1 (low), 10 μg L−1 (medium), and 100 μg L−1 (high). Organisms were exposed for 10 days followed by 10 days of depuration in clean seawater under controlled laboratory conditions. The evaluation of MP effects on mussel clearance rate, tissue structure, antioxidant defenses, immune and digestive parameters, and DNA integrity were investigated while the identification of plastic particles in mussel tissues (gills, digestive gland, and remaining tissues), and biodeposits (feces and...
Tipo: Text
Palavras-chave: Microplastics; Polyethylene; Polypropylene; Mytilus; Oxidative stress; Biomarkers.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00489/60089/63427.pdf

Evaluation du probiotique bactérien Pediococcus acidilactici MA18/5M chez la crevette pénéide Litopenaeus stylirostris en Nouvelle- Calédonie
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Castex, Mathieu.
P. acidilactici MA 18/5M is a dietary probiotic (BACTOCELL®) which has shown its efficiency on several terrestrial and aquatic species. The present thesis aimed to investigate more fully the effects of this probiotic on cultured penaeid shrimps. For this study, we chose a global approach by replacing the problem studied in a model of interactions between three compartments: the shrimp, its intestinal microflora and the probiotic. Having first defined how the probiotic should be administrated, we studied its effects under controlled laboratory conditions at several levels of Litopenaeus stylirostris biology: (i) Nutritional status and growth, (ii) associated bacterial microflora, and (iii) health status with respect to oxidative stress. Then the probiotic...
Tipo: Text
Palavras-chave: Floating cages; Vibriosis; Oxidative stress; Intestinal microflora; Nutrition; Growth; Shrimp; Pediococcus acidilactici; Probiotic; Cages flottantes; Vibriose; Stress oxydant; Microflore intestinale; Nutrition; Croissance; Crevette; Pediococcus acidilactici; Probiotique.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/these-6814.pdf

Behavioural and biochemical responses of two marine invertebrates Scrobicularia plana and Hediste diversicolor to copper oxide nanoparticles
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Buffet, Pierre-emmanuel; Tankoua, Olivia Fossi; Pan, Jin-fen; Berhanu, Deborah; Herrenknecht, Christine; Poirier, Laurence; Amiard-triquet, Claude; Amiard, Jean-claude; Berard, Jean-baptiste; Risso, Christine; Guibbolini, Marielle; Romeo, Michele; Reip, Paul; Valsami-jones, Eugenia; Mouneyrac, Catherine.
Engineered nano-sized Cu oxide particles are extensively used in diverse applications. Because aquatic environments are the ultimate "sink" for all contaminants, it is expected that nanoparticles (NP) will follow the same fate. In this study, two marine invertebrates Scrobicularia plana and Hediste diversicolor were chosen as ecotoxicological models. The aim was to evaluate behavioural (burrowing kinetics, feeding rate) and biochemical (biomarkers) responses of S. plana and H. diversicolor exposed in the laboratory to Cu (10 mu g L(-1)) added in natural seawater either in the form of engineered nanoparticles (NPs) of CuO or as dissolved Cu in 2% HNO(3). Exposure was characterized by considering (i) the physico-chemical fate of NP (ii) the fraction of...
Tipo: Text
Palavras-chave: Copper oxide nanoparticles; Behaviour; Biomarkers; Oxidative stress; Scrobicularia plana; Hediste diversicolor.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00040/15140/15856.pdf

Physiological and biochemical changes associated with massive mortality events occurring in larvae of American oyster (Crassostrea virginica)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Genard, Bertrand; Pernet, Fabrice; Lemarchand, Karine; Boudry, Pierre; Moraga, Dario; Tremblay, Rejean.
In this paper, biochemical and physiological analyses were used to characterize changes associated with mortality event occurred during veliger development of American oyster, Crassostrea virginica. Biochemical analyses included the evaluation of lipid classes, fatty acid composition and total protein content. Larval physiology was evaluated by studying feeding activity, enzymes related to energy metabolism, oxidative stress levels and enzymatic antioxidant defenses. These analyses were complemented by bacterial community analyses as well as by measuring larval oyster performance. We observed that mortality events coincided with (1) strong changes in the surrounding bacterial community; (2) a progressive decrease in feeding activity; (3) higher levels of...
Tipo: Text
Palavras-chave: Massive mortality; Larvae; Microbial environment; Lipid; Fatty acids; Energy metabolism; Oxidative stress; Antioxidant enzymes; Crassostrea virginica.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00051/16231/13753.pdf

Starting with a handicap: phenotypic differences between early- and late-born king penguin chicks and their survival correlates
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Stier, Antoine; Viblanc, Vincent A.; Massemin-challet, Sylvie; Handrich, Yves; Zahn, Sandrine; Rojas, Emilio R.; Saraux, Claire; Le Vaillant, Maryline; Prud'Homme, Onesime; Grosbellet, Edith; Robin, Jean-patrice; Bize, Pierre; Criscuolo, Francois.
1.  The exceptionally long (c. 11 months) growth period of king penguin chicks (Aptenodytes patagonicus) is interrupted by the Austral winter. As a consequence, penguin chicks born late in the breeding season have little time to build-up their energy reserves before the drastic energy bottleneck they experience during winter and face greater risks of mortality than early-born chicks.  2.  Whereas it is well known that breeding adults alternate between early- and late-breeding attempts, little is known on the phenotype of early- and late-chicks, and on the potential existence of specific adaptive phenotypic responses in late-born individuals.  3.  We investigated phenotypic differences between early- and late-chicks and tested their survival correlates both...
Tipo: Text
Palavras-chave: Early-life conditions; Phenotypic plasticity; Oxidative stress; Individual quality; Growth; Telomere; Corticosterone; Reproductive timing.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00170/28175/32107.pdf

Protective Role of Melatonin in Mouse Spermatogenesis Induced by Sodium Arsenite
Provedor de dados: International Journal of Morphology
Autores: Bustos-Obregón,Eduardo; Poblete,Daniel; Catriao,Roberto; Fernandes,Fábio Henrique.
Arsenic is a testicular environmental toxic. Melatonin (Me), being a potent antioxidant, may reduce the damage caused by arsenic in male fertility. The effects of daily oral exposure of Sodium Arsenite (As; 7.0 mg/kg/bw); Melatonin (Me, 10.0 mg/kg/bw); Me (10.0 mg/kg/bw) plus As (7.0 mg/kg/bw), and Negative Control (NaCl 0.9%) in male CF-1 adult mice were assessed in acute (8.3 days), chronic (33.2 days) and recovery (66,4 days) of testicular damage. We evaluated changes in testicular weight and histopathological, morphometric measurements, expression of COX-2 and Androgen Receptor (AR) antigens and lipid peroxidation levels. Treatment resulted in decreased tubular diameter and AR expression, and increased: interstitial area, luminal diameter, COX-2...
Tipo: Journal article
Palavras-chave: Mouse; Arsenic; COX-2; Androgen receptor; Oxidative stress; Melatonin.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300012

Gill and Liver Histopathology in Goodea atripinnis Jordan, Related to Oxidative Stress in Yuriria Lake, Mexico
Provedor de dados: International Journal of Morphology
Autores: Ruiz-Picos,R; López-López,E.
In aquatic ecosystems, the complex mixture of pollutants may mediate the formation of free radicals and cause oxidative damage to the biota. Yuriria Lake (a Ramsar site in Central Mexico) receives input of wastewater from its tributaries, agricultural runoff, and municipal discharge. We studied the lipid peroxidation (LPO), antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), and histopathology of gill and liver of the native fish Goodea atripinnis in Yuriria Lake. Results were compared to a control group of fish cultivated in the laboratory. LPO, SOD, and CAT showed no significant differences compared to controls, but GPx showed greater and significant differences in both tissues. Three class sizes were...
Tipo: Journal article
Palavras-chave: Mexican wetlands; Oxidative stress; Histopathology; Fish health condition; Liver; Gill.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300060

Analysis of Myenteric Neurons of the Cecum of Diabetic Rats After Supplementation with Ascorbic Acid
Provedor de dados: International Journal of Morphology
Autores: Zanoni,Jacqueline Nelisis; Pereira,Priscila Goncalves Cardoso; dos Santos Pereira,Marli Aparecida.
The objective of this work was to investigate the neuroprotective action of the ascorbic acid over the myenteric neurons in the cecum of Wistar rats, four months after induction of the diabetes mellitus experimental with streptozotocin. Three groups with five rats each were used: C- controls, D- diabetic, DA- diabetic treated with ascorbic acid. For evidentiation of the myenteric neurons was carried out to Giemsa's technique. Were evaluated the areas of cell bodies of 500 neurons in each group studied. The quantitative analysis was carried out in an area of 16.6 mm2 in each cecum studied. In the animals diabetic observed elevation of the glycemia and glycated hemoglobin. The supplementation with ascorbic acid was effective under the myenteric neurons of...
Tipo: Journal article
Palavras-chave: Ascorbic acid; Diabetes mellitus; Oxidative stress; Cecum; Streptozotocin.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200013

Protective Role of Melatonin and Taurine Against Carbamazepine-induced Toxicity in Freshly Isolated Rat Hepatocytes
Provedor de dados: International Journal of Morphology
Autores: Eghbal,Mohammad Ali; Taziki,Shohreh; Sattari,Mohammad Reza.
Carbamazepine is widely used in a broad spectrum of psychiatric and neurological disorders. Idiosyncratic hepatotoxicity is a well-known adverse reaction associated with carbamazepine. Hepatotoxicity is rare, but a real concern when initiating therapy. It was found that oxidative stress is a potential mechanism for carbamazepine-induced hepatotoxicity. Present study evaluated the hepato protective role of taurine and melatonin against carbamazepine-induced hepatotoxicity. Hepatocytes were prepared by the method of collagenase enzyme perfusion via portal vein. Cells were treated with 400 µM carbamazepine, 1mM taurine, and 1mM melatonin. Cell death, reactive oxygen species formation, lipid peroxidation, and mitochondrial membrane depolarization were assessed...
Tipo: Journal article
Palavras-chave: Carbamazepine; Hepatotoxicity; Oxidative stress; Taurine; Melatonin.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300049

Density and Morphometry of Overall Myenteric Neurons Population from the Duodenum of Diabetic Rats (Rattus norvegicus) under Ascorbic Acid Supplementation
Provedor de dados: International Journal of Morphology
Autores: Pereira,Marli Aparecida dos Santos; Liberati,Ana Paula; Souza,Ivan Domicio da Silva; Zanoni,Jacqueline Nelisis.
The objective of this work was to evaluate the overall myenteric neurons population from the duodenum of adult streptozotocin-induced diabetic rats supplemented with ascorbic acid (AA), a potent antioxidant. Fifteen 90-day-old rats were divided in groups: control (C), diabetic (D), diabetic treated with ascorbic acid (DA). After 120 days of experimental period duodenums were resected and processed as whole-mount preparations according to Giemsa's technique, which allowed us to evaluate neuronal density in an area of 8.96 mm² and measure the area of 500 neuronal cell bodies per group. It was observed a 32.55% reduction in neuronal density of group D when compared to group C (p&lt;0.05). The density of spared neurons in group DA, in relation to group D, was...
Tipo: Journal article
Palavras-chave: Ascorbic acid; Diabetes mellitus; Duodenum; Oxidative stress.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200017

Diabetes-Induced Prefrontal Nissl Substance Deficit and the Effects of Neem-Bitter Leaf Extract Treatment
Provedor de dados: International Journal of Morphology
Autores: Akinola,Oluwole B; Omotoso,Olaiya G; Dosumu,Olufunke O; Akinola,Oluwafunmike S; Olotufore,Favour.
Cognitive dysfunction is reportedly associated with poorly-managed diabetes mellitus. In this study, we report the effect of oral treatment with combined leaf extract (CLE) of neem and bitter leaf on the prefrontal cortex of diabetic Wistar rats. Adult male Wistar rats were randomized to one of the following groups: control, diabetic (STZ-induced), STZ + CLE, STZ + metformin and CLE only. At euthanasia, paraffin sections of the prefrontal cortex were stained with cresyl fast violet; while malondialdehyde (MDA) and glutathione peroxidase (GPx) were assayed in prefrontal homogenates. Oral CLE produced normoglycemia in the treated hyperglycaemic rats. Besides, Nissl-stained prefrontal sections showed no morphologic deficits in all the groups except the...
Tipo: Journal article
Palavras-chave: Pyramidal cells; Hyperglycaemia; Oxidative stress; Cognition.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300031

Potential Radioprotective Effect of AT1 Receptor Antagonists Against Morphological and Ultrastructural Changes in the Testes Induced by Ionizing Radiation
Provedor de dados: International Journal of Morphology
Autores: Schwarz,Franciele Cristina; Mansano,Naira da Silva; Chies,Agnaldo Bruno; Viani,Gustavo Arruda; Spadella,Maria Angélica.
Radiotherapy is a source of human exposure to ionizing radiation. This pure energy causes deleterious effects on tissues, which result from oxidative stress, a phenomenon in which there is the participation of the Renin-Angiotensin System (RAS). The male genital organs are extremely radiosensitive and the action of radiation in the testes can significantly affect spermatogenesis. In search of potential radioprotective for male genital system, this study investigated whether the AT1 receptor antagonists minimize radiation-induced damage to reproductive tissues, by decreasing oxidative stress. Male Wistar rats were divided into six groups: 0 Gray (Gy) (control), 5 Gy (single dose in the scrotal area), telmisartan, losartan, 5Gy+telmisartan and 5Gy+losartan....
Tipo: Journal article
Palavras-chave: Ionizing radiation; Testicular ultrastructure; Spermatogenesis; Renin-angiotensin system; Oxidative stress; Angiotensin II type 1 receptor blockers.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000300005

The Role of Antioxidants in Cerebellar Development: A Review of Literature
Provedor de dados: International Journal of Morphology
Autores: Imosemi,I. O.
Developmental pathologies may result from endogenous or xenobiotic-enhanced formation of reacting oxygen species (ROS), which oxidatively damage cellular macromolecules and/or alter signal transduction, and that the embryonic processes regulating the balance of ROS formation, oxidative DNA damage and repair, and ROS-mediated signal transduction may be important determinants of teratological risk. ROS can oxidize molecular targets such as DNA, protein, lipid in a process called oxidative stress resulting in cellular dysfunction and in utero death or teratogenicity. This review, consisting of literature search of journals and chapters in books aims at highlighting the importance of the cerebellum in controlling various motor activities in the body, as well...
Tipo: Journal article
Palavras-chave: Cerebellar development; Reacting oxygen species; Oxidative stress; Antioxidants.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100034

Cytotoxic activity of Androctonus australis hector venom and its toxic fractions on human lung cancer cell line
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Béchohra,Louisa; Laraba-Djebari,Fatima; Hammoudi-Triki,Djelila.
Abstract  Background: Several studies have showed that animal venoms are a source of bioactive compounds that may inhibit the growth of cancer cells, which makes them useful agents for therapeutic applications. Recently, it was established that venom toxins from scorpions induced cytotoxic, antiproliferative and apoptogenic effects on cancer cells. Therefore, the present study aims to investigate the cytotoxic activity of Androctonus australis hector (Aah) scorpion venom and its toxic fractions (FtoxG-50 and F3) on NCI-H358 human lung cancer cells.  Methods: The cytotoxic and antiproliferative activities were estimated using MTT assay, lactate dehydrogenase release and clonogenic assays. Apoptosis was evaluated by Hoechst 33258 staining, DNA fragmentation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aah venom; Cytotoxicity; F3 fraction; Apoptosis; Lung cancer cells; Oxidative stress.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100322

Rattlesnake Crotalus molossus nigrescens venom induces oxidative stress on human erythrocytes
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Meléndez-Martínez,David; Muñoz,Juan Manuel; Barraza-Garza,Guillermo; Cruz-Peréz,Martha Sandra; Gatica-Colima,Ana; Alvarez-Parrilla,Emilio; Plenge-Tellechea,Luis Fernando.
Abstract  Background  Globally, snake envenomation is a well-known cause of death and morbidity. In many cases of snakebite, myonecrosis, dermonecrosis, hemorrhage and neurotoxicity are present. Some of these symptoms may be provoked by the envenomation itself, but others are secondary effects of the produced oxidative stress that enhances the damage produced by the venom toxins. The only oxidative stress effect known in blood is the change in oxidation number of Fe (from ferrous to ferric) in hemoglobin, generating methemoglobin but not in other macromolecules. Currently, the effects of the overproduction of methemoglobin derived from snake venom are not extensively recorded. Therefore, the present study aims to describe the oxidative stress induced by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Attenuated total reflectance-Fourier transform infrared spectroscopy; Crotalus molossus nigrescens; Venom; Snake venom; Methemoglobin; Oxidative stress; Oxyhemoglobin.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100311

Hepatotoxicity and oxidative stress induced byNaja haje crude venom
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Al-Quraishy,Saleh; Dkhil,Mahamed A; Moneim,Ahmed Esmat Abdel.
Background Snake venoms are synthesized and stored in venom glands. Most venoms are complex mixtures of several proteins, peptides, enzymes, toxins and non-protein components. In the present study, we investigated the oxidative stress and apoptosis in rat liver cells provoked by Naja haje crude injection (LD50) after four hours.Methods Wistar rats were randomly divided into two groups, the control group was intraperitoneally injected with saline solution while LD50-dose envenomed group was intraperitoneally injected with venom at a dose of 0.025 μg/kg of body weight. Animals were killed four hours after the injection. Lipid peroxidation, nitric oxide and glutathione levels were measured as oxidative markers in serum and liver homogenate. In addition, liver...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Naja haje venom; Hepatotoxicity; Oxidative stress; Apoptosis; Rats.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200314

Conus vexillum venom induces oxidative stress in Ehrlich's ascites carcinoma cells: an insight into the mechanism of induction
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Abdel-Rahman,Mohamed A; Abdel-Nabi,Ismail M; El-Naggar,Mohamed S; Abbas,Osama A; Strong,Peter N.
Background It is estimated that venoms of marine cone snails (genus Conus) contain more than 100,000 different small peptides with a wide range of pharmacological and biological actions. Some of these peptides were developed into potential therapeutic agents and as molecular tools to understand biological functions of nervous and cardiovascular systems. In this study we examined the cytotoxic and anticancer properties of the marine vermivorous cone snail Conus vexillum (collected from Hurgada and Sharm El-Shaikh, Red Sea, Egypt) and suggest the possible mechanisms involved. The in vitro cytotoxic effects of Conus venom were assessed against Ehrlich’s ascites carcinoma (EAC) cells. Results Conus venom treatment resulted in concentration-dependent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conus vexillum venom; Ehrlich’s cells; Oxidative stress; Cancer; Egypt.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100307

Antiproliferative activity of marine stingray Dasyatis sephenvenom on human cervical carcinoma cell line
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Rajeshkumar,RK; Vennila,R; Karthikeyan,S; Prasad,N Rajendra; Arumugam,M; Velpandian,T; Balasubramaniam,T.
AbstractBackgroundVenoms comprise mixtures of numerous bioactive compounds that have a wide range of pharmacologic actions. Toxins from venomous animals have attracted the attention of researchers because of their affinity for primary sites responsible for lethality and their efficacy at extremely low concentrations. The venoms of marine stingrays have not been extensively studied and limited data is available on them. The present study aims to evaluate the antiproliferative and biochemical properties of the venom obtained from a species of marine stingray (Dasyatis sephen) on human cervical cancer cell line HeLa.MethodsThe antiproliferative effect of D. sephen venom was determined by MTT assay, and the oxidative stress was determined by lipid peroxidation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marine organisms; Membrane potential; Oxidative stress; ROS; Stingray; Venom.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100345

Oxidative stress and hepatotoxicity in rats induced by poisonous pufferfish (Lagocephalus lagocephalus) meat
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Saoudi,M; Abdelmouleh,A; Ellouze,F; Jamoussi,K; El Feki,A.
This study was undertaken to evaluate the effect of pufferfish (Lagocephalus lagocephalus) meat poisoning on hepatic functions of Wistar rats. For this purpose, groups of rats (Lcr, Lcu+b and Lcu-b) received diet supplemented with 10% of raw or cooked meat, respectively, with or without cooking water of L. lagocephalus while groups Mcr and Mcu+b received diet supplemented with 10% of raw or cooked meat of Liza aurata, which were used as a negative control. In Lcu+b group, ALT, AST and ALP rates (hepatic enzyme markers) decreased after two months of treatment, indicating liver damage. We also observed an increase of 54 and 65% of thiobarbituric acid reactive substances (TBARS) in their livers respectively 48 hours and two months after treatment compared to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatotoxicity; Lagocephalus lagocephalus; Oxidative stress; Tetrodotoxin.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300006

The essential oil from Lippia alba induces biochemical stress in the silver catfish (Rhamdia quelen) after transportation
Provedor de dados: Neotropical Ichthyology
Autores: Salbego,Joseânia; Becker,Alexssandro G.; Gonçalves,Jamile F.; Menezes,Charlene C.; Heldwein,Clarissa G.; Spanevello,Rosélia M.; Loro,Vania L.; Schetinger,Maria Rosa C.; Morsch,Vera M.; Heinzmann,Berta M.; Baldisserotto,Bernardo.
This study investigated the effects of the essential oil (EO) from Lippia alba on biochemical parameters related to oxidative stress in the brain and liver of silver catfish (Rhamdia quelen) after six hours of transport. Fish were transported in plastic bags and divided into three treatments groups: control, 30 µL L- 1 EO from L.alba and 40 µL L-1 EO from L.alba. Prior to transport, the fish were treated with the EO from L. alba (200 µL L -1 for three minutes), except for the control group. Fish transported in bags containing the EO did not have any alterations in acetylcholinesterase, ecto -nucleoside triphosphate diphosphohydrolase and 5'nucleotidase activity in the brain or superoxide dismutase activity in the liver. The hepatic catalase (CAT),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anesthetics; Antioxidant defenses; Biochemical parameters; Fish transport; Oxidative stress.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000400811

Probucol dramatically enhances dihydroartemisinin effect in murine malaria
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Kume, Aiko; Dang Trinh Minh Anh; Shichiri, Mototada; Ishida, Noriko; Suzuki, Hiroshi.
Background: Artemisinin-based combination therapy (ACT) has been adopted as national policy for the first-line treatment in large number of malaria-endemic regions. However, artemisinin-resistant parasites have emerged and are spreading, with slow-cleaning parasites being reported in patients treated with ACT. It means that more parasites are exposed to the partner drug alone and the risk of developing resistant parasites against the partner drug is increasing. Therefore, the development of a new method to enhance the effect of artemisinin is required. In this study, the potential effect of probucol as a combination drug of dihydroartemisinin (DHA), an artemisinin derivative, was examined. Methods: C57BL/6 J mice infected with Plasmodium yoelii XL-17 were...
Palavras-chave: C57BL/6 J mice; Dihydroartemisinin; Malaria; Oxidative stress; Probucol; Plasmodium yoelii XL-17; Vitamin E.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4436

Growth, antioxidant system, and immunological status of shrimp in bioflocs and clear water culture systems
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Souza,Diego Moreira de; Kutter,Mateus Tavares; Furtado,Plinio Schmidt; Romano,Luis Alberto; Wasielesky Junior,Wilson; Monserrat,José María; Garcia,Luciano.
Abstract: The objective of this work was to evaluate the effect of the traditional culture system in clear water and of the one in microbial flocs on the antioxidant and immunological status of Litopenaeus vannamei shrimp. Samples (gill, hemolymph, and hepatopancreas) were collected on days 15, 30, and 60 of the experimental period. The following immunological parameters were analyzed at each evaluation time: granular and hyaline hemocytes, total protein, and apoptosis. Assays on glutamate-cysteine ligase (GCL) activity and on the concentrations of reduced glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) were also performed. The evaluated immunological parameters did not differ significantly between treatments. Shrimp reared in clear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Litopenaeus vannamei; BFT; Oxidative stress; TBARS.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000101100

Effects of high arsenic and fluoride soil concentrations on soybean plants
Provedor de dados: Phyton: Revista Internacional de Botánica Experimental
Autores: Bustingorri,C; Balestrasse,K; Lavado,RS.
Arsenic (As) and Fluoride (F) are present in many soils, affecting crops and posing risks in the food chain. We performed pot experiments on spiked soils enriched in these elements either individually or simultaneously, over a wide range of concentrations. Soybean biomass production, grain yield, As and F accumulation and distribution within the plant, and the antioxidant response to these stresses were analyzed. Arsenic was more toxic than F. At As levels &gt;35 mg/kg and F levels &gt;375 mg/kg, yield loss reached 60% and 30%, respectively. At the highest dose of As plants died within 2 weeks, whereas F showed no lethality. When they were applied simultaneously, detrimental effects were more important. As and F in plants increased in all soybean organs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soil contamination; Toxic elements in crops; Grain contamination; Oxidative stress; Soybean.
Ano: 2015
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572015000200020

Physiological responses of Allium cepa var. agrogarum L. plants to Cadmium stress
Provedor de dados: Phyton: Revista Internacional de Botánica Experimental
Autores: Wang,QL; Liu,DH; Yue,JY.
This study aimed to determine the effects of different Cd concentrations (0, 1, 10, 100, and 300 µM CdCl2) on Allium plantlets. To achieve this goal, we examined the (1) dynamics of Cd2+ fux in the rhizosphere, (2) partitioning of Cd between roots and leaves, (3) formation of reactive oxygen species (ROS), (4) levels of H2O2 and chlorophyll, and (5) translocation of several macro- and micronutrients. A strong net Cd2+ influx was observed in the root apex after exposure to 100 µM Cd for 24 h. Exposure to either 1 or 10 µM Cd for 96 h presented no evident influence on root growth. However, treatments with 100 to 300 µM Cd for 72 h significantly inhibited root growth. ROS levels increased in roots and leaves with increasing Cd concentrations. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cadmium (Cd) toxicity; Net cadmium flux; ICP; Oxidative stress; Photosynthesis.
Ano: 2016
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572016000200015

Interference of Volunteer Corn on Stress Metabolism and Yield of Dry Beans
Provedor de dados: Planta Daninha
Autores: PIASECKI,C.; RIZZARDI,M.A.; SCHONS,J.; CAVERZAN,A.; OLIVEIRA,C.
ABSTRACT: Dry bean cultivation after corn favors the occurrence of volunteer corn plants which interfere with the crop and cause yield losses of dry bean. Yield losses resulting from interferences caused by corn may be related to oxidative stress, which, in turn, is caused by the higher production of reactive oxygen species (ROS), such as hydrogen peroxide (H2O2). This work aimed to quantify H2O2 contents, and the activity of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) in dry beans under interference with densities of volunteer corn F2 originated from individual plants and clumps (seven corn plants emerged at the same point). Two experiments were carried out in a randomized blocks design with three replicates in Passo Fundo -...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Zea mays; Clumps; Oxidative stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100310

Morphological and Biochemical Alterations of Paddy Rice in Response to Stress Caused by Herbicides and Total Plant Submersion
Provedor de dados: Planta Daninha
Autores: MARCHEZAN,M.G.; AVILA,L.A.; AGOSTINETTO,D.; SCHAEDLER,C.E.; LANGARO,A.C.; OLIVEIRA,C.; ZIMMER,M.; SCHREIBER,F.
ABSTRACT Herbicide selectivity in paddy rice varies in several aspects, among which are the environmental conditions. The aim of the study was to evaluate the effect of herbicide application and total plant submersion on morphological and biochemical changes in paddy rice. Total chlorophyll and carotenoids, catalase activity, ascorbate peroxidase and superoxide dismutase, total phenolic content, lipid peroxidation and hydrogen peroxide levels were assessed. Leaf samples were collected 24 hours and 7 days after the application of water regimes. The results observed in the first experiment show that cultivars Puitá INTA CL, IRGA 417 and IRGA 422 CL are more tolerant to total submersion. The most sensitive cultivars are IRGA 424, BRS Querência, EPAGRI 108 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Hypoxia; Oryza sativa; Herbicide selectivity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582017000100201

Biochemical and Physiological Changes in Rice Plants Due to the Application of Herbicides1
Provedor de dados: Planta Daninha
Autores: LANGARO,A.C.; AGOSTINETTO,D.; OLIVEIRA,C.; SILVA,J.D.G.; BRUNO,M.S.
ABSTRACT The application of herbicides, even if selective, can cause biochemical and physiological changes, resulting in oxidative stress. This stress comes from the accumulation of reactive oxygen species produced due to exposure to the herbicide. However, plants have developed defense strategies, which can be enzymatic or non-enzymatic. The objective of this study was to evaluate the morphological and metabolic changes such as photosynthetic parameters, oxidative damage and antioxidant enzyme activity of rice plants after applying herbicides. For this, a study was conducted in a greenhouse and laboratory and the treatments consisted of application of imazapic + imazapyr, quinclorac, bentazon, cyhalofop-butyl, penoxsulan, bispyribac-sodium and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oryza sativa; Oxidative stress; Lipid peroxidation; Selectivity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000200277

Changes in the Metabolism of Soybean Plants Submitted to Herbicide Application in Different Weed Management Systems
Provedor de dados: Planta Daninha
Autores: FRAGA,D.S.; AGOSTINETTO,D.; RUCHEL,Q.; LANGARO,A. C.; OLIVEIRA,C.
ABSTRACT: Technologies that advocate the use of herbicide-resistant crops are alternatives to weed control, but they may cause oxidative stress and change secondary metabolism of plants. Therefore, this study aimed to evaluate changes in the secondary metabolism of soybean plants which contained Cultivance® (CV), sulfonylurea-tolerant soybean (STS) and Roundup Ready® (RR) technologies submitted to the application of the mixture of herbicides imazapyr and imazapic in “plant-apply” and “apply-plant” management systems. Two field experiments, in which soybean cultivars were submitted to increasing doses of the mixture of herbicides imazapyr and imazapic, were performed. Aerial parts of plants were collected 10 days after crop emergence and stored at -83oC,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Oxidative stress; Chemical control.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582020000100243

Morphological and physiological alterations induced by lactofen in soybean leaves are reduced with nitric oxide
Provedor de dados: Planta Daninha
Autores: Ferreira,L.C; Cataneo,A.C; Remaeh,L.M.R; Búfalo,J; Scavroni,J; Andréo-Souza,Y; Cechin,I; Soares,B.J.A.
Lactofen is a diphenylether herbicide recommended to control broad-leaved weeds in soybean (Glycine max) fields and its mechanism of action is the inhibition of protoporphyrinogen-IX oxidase (Protox), which acts in the chlorophyll biosynthesis. This inhibition results in an accumulation of protoporphyrin-IX, which leads to the production of reactive oxygen species (ROS) that cause oxidative stress. Consequently, spots, wrinkling and leaf burn may occur, resulting in a transitory crop growth interruption. However, nitric oxide (NO) acts as an antioxidant in direct ROS scavenging. Thus, the aim of this work was to verify, through phytometric and biochemical evaluations, the protective effect of NO in soybean plants treated with the herbicide lactofen....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Oxidative stress; Plant growth; Soluble sugars.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582011000400014

Physiological and Molecular Responses in Rice, Weedy Rice and Barnyardgrass Exposed to Supra-Optimal Temperatures
Provedor de dados: Planta Daninha
Autores: OLIVEIRA,C.; AGOSTINETTO,D.; LANGARO,A.C.; GARCIA,J.R.; LAMEGO,F.P.
ABSTRACT: The global temperature to rise 0.3 to 4.8 oC to century. Supra-optimal temperatures can affect plants at different organizational levels, causing morphological, physiological, biochemical and gene expression alterations. Rice, weedy rice, and barnyardgrass may to response differently when subjected to supra-optimal temperatures. Thus, the aimed at determining the physiological response and expression of the genes APX2, HSP24.15 e HSP71.10 in rice, weedy rice, and barnyardgrass when in to supra-optimal temperatures. A greenhouse experiment was conducted in randomized complete desing with four repetitions, with a factorial combination of temperature x plantas, where: factor A consisted of two temperatures (25 oC and 40 oC); and factor B of three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oryza sativa; Echinochloa spp.; Photosynthesis; Oxidative stress; Gene expression.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100239

CHANGE IN PHYSIOLOGICAL FEATURES IN RYEGRASS BIOTYPES IN COMPETITION WITH SOYBEAN DUE RESISTANCE TO GLYPHOSATE
Provedor de dados: Planta Daninha
Autores: AGOSTINETTO,D.; OLIVEIRA,C.; LANGARO,A.C.; NOHATTO,M.A.; MANICA-BERTO,R.
ABSTRACT Herbicide resistance can cause biochemical, physiological, morphological and phenological changes in weeds, altering their competitive ability against crops. The aim of the study was to assess physiological changes and cellular damage in susceptible and resistant biotypes of ryegrass and soybeans under competition. Three experiments were conducted in a greenhouse using a completely randomized design with four replications performed in a replacement series. The ratios of soybean and to susceptible (experiment 1) and resistant ryegrass (experiment 2) and of susceptible to resistant (experiment 3) ryegrass were 100:0, 75:25, 50:50, 25:75 and 0:100. Forty days after the emergence of the soybean crop and 60 days after the emergence of the ryegrass...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Antioxidant enzymes; Adaptability of herbicide resistant plants.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000300517

Responses of Plants to Pesticide Toxicity: an Overview
Provedor de dados: Planta Daninha
Autores: SHARMA,A.; KUMAR,V.; THUKRAL,A.K.; BHARDWAJ,R.
ABSTRACT: Pesticides are applied all over the world to protect plants from pests. However, their application also causes toxicity to plants, which negatively affects the growth and development of plants. Pesticide toxicity results in reduction of chlorophyll and protein contents, accompanied by decreased photosynthetic efficiency of plants. Pesticide stress also generates reactive oxygen species which causes oxidative stress to plants. To attenuate the negative effects of oxidative stress, the antioxidative defense system of plants gets activated, and it includes enzymatic antioxidants as well as non-enzymatic antioxidants. The present review gives an overview of various physiological responses of plants under pesticide toxicity in tabulated form.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Physiological responses; Antioxidative defense system; Oxidative stress.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100303

Biochemical Alterations of Weeds in Response to Stress Caused by Herbicides and Total Plant Submersion
Provedor de dados: Planta Daninha
Autores: MARCHEZAN,M.G.; AVILA,L.A.; SCHAEDLER,C.E.; AMARANTE,L.; SCHERNER,A.; SCHREIBER,F.
ABSTRACT: The maximum biological efficiency of a herbicide is performed when the plants are in favorable condition to absorb and metabolize it. Stress situations, such as total submersion, cause stress in plants, reducing weed control efficiency and crop yield. The objective of this study was to verify whether different soil water content and herbicide treatments alter the components of the secondary metabolism and cause cellular damage in weed species Two experiments were conducted (I and II) in a completely randomized experimental design with a factorial scheme (2 x 2 x 4 and 2 x 5, respectively) and three replicates. The first experiment was conducted with two species (Leersia hexandra and Luziola peruviana), which were submitted to four herbicide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Luziola peruviana; Leersia hexandra; Echinochloa crus-galli; Oxidative stress; Chemical control.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582017000100288

Does the Interference of GR® Volunteer Corn Alters Stress Metabolism on Soybean?
Provedor de dados: Planta Daninha
Autores: PIASECKI,C.; RIZZARDI,M.A.; SCHONS,J.; CAVERZAN,A.; CHAVARRIA,G.
ABSTRACT: The cultivation of GR® corn prior to soybean favors the occurrence of GR® volunteer corn plants interfering in soybean crops. The interference of volunteer corn causes the soybean yield losses, and the magnitude of losses varies with the corn density. The soybean yield losses can be partially explained by the occurrence of oxidative stress, which occurs by the higher content of reactive oxygen species (ROS), such as hydrogen peroxide (H2O2). The objective of this study was to quantify H2O2 content and the activity of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) on soybean as a function of interference of populations of GR® volunteer corn originated from individual plants and clumps (clumps are seven corn plants...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Zea mays; Volunteer plants; Competition; Oxidative stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100219

The Effect of High Temperature on Viability, Proliferation, Apoptosis and Anti-oxidant Status of Chicken Embryonic Fibroblast Cells
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Ibtisham,F; Zhao,Y; Nawab,A; Liguang,H; Wu,J; Xiao,M; Zhao,Z; An,L.
ABSTRACT The effects of oxidative stress induced by high temperature on the cell viability, proliferation, apoptosis and oxidative status of chicken embryonic fibroblasts (CEF) were analyzed. The viability, proliferation, apoptotic and anti-oxidative status were measured after incubating CEF at the temperatures of 37ºC (control) and 40-44ºC (experimental groups) for 6,12 and 24 hours. The results showed that at high temperature (42-43ºC), the viability of CEF cells decreased after 6, 12 and 24 h of incubation, but the difference was significant only at 43ºC. Cell proliferation was significantly reduced at 44oC/6h. The apoptotic rate of CEF cells was increased following heat treatments in a time-dependent manner. ROS formation increased with increasing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Chicken embryonic fibroblast; Cell proliferation; Heat stress; Oxidative stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300463

Effect of Two Strains of Probiotics on the Antioxidant Capacity, Oxidative Stress, and Immune Responses of Salmonella-Challenged Broilers
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Abudabos,AM; Alyemni,AH; Zakaria,HAH.
Abstract The present study aimed at evaluating the effect of two strains of probiotics (Bacillus subtilis PB6 and Bacillus Cereus var. toyoi) on selected indicators of the antioxidant status and immune responses of broilers before and after challenge with Salmonella enterica subsp. Typhimurium (S. typhimurium). Birds were distributed into the following five treatments: negative control (CTL−); negative control+S. typhimurium (CTL+); antibiotic+S. typhimurium, (AB); Toyocerin(r)+S. typhimurium (TOYO); and CloSTATTM+ S. typhimurium (CLOS). Birds in all treatments, except for CTL−, were orally challenged with 3 x109 CFUS. typhimurium at 16 days of age. The results showed that total antioxidant capacity (TAC), superoxide dismutase activity (SOD), and hydrogen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immune responses; Probiotics; Oxidative stress; Salmonella enterica; Total antioxidant capacity (TAC).
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000100175

Effect of Oxidized Soybean Oils on Oxidative Status and Intestinal Barrier Function in Broiler Chickens
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Tan,L; Rong,D; Yang,Y; Zhang,B.
ABSTRACT The objective of this study was to evaluate the effect of oxidized soybean oils on the growth performance, metabolic oxidative status and intestinal barrier function of broiler chickens. A total of 240 one-day-old female broiler chickens were assigned to four dietary treatments with six replicates (cages) of 10 birds each. The dietary treatments comprised of a basal diet supplemented with 4% of: non-oxidized (fresh) soybean oil (control treatment, SNX); lowly-oxidized soybean oil (SLX) (oil heated for 10h at 200°C); moderately-oxidized soybean oil (SMX) (oil heated for 18h at 200°C); or highly-oxidized soybean oil (SHX) (oil heated for 30h at 200°C). Diets and water were offered ad libitum. The experiment was lasted 21d.The growth performance of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal barrier function; Inflammation-related factor; Oxidative stress; Heat-oxidized oil; Broilers.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000200333

Effect of Vitamin E Supplementation and High Stocking Density on the Performance and Stress Parameters of Broilers
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Selvam,R; Saravanakumar,M; Suresh,S; Sureshbabu,G; Sasikumar,M; Prashanth,D.
ABSTRACT The present study was carried out to investigate the effect of dietary supplementation with vitamin E and stocking density on the mortality, body weight gain, feed conversion ratio, European production efficiency factor, heterophil/lymphocyte (H/L) ratio and liver glutathione (GSH) and malondialdehyde (MDA) levels of broilers. In total, 570 one-day-old Vencobb 400 broiler chickens were randomly assigned to three treatments, with six replicates each. G1 served as normal control and birds were provided a normal floor space (0.0465 m2/bird from day 1 to 21 and 0.0929 m2/bird from day 22 to 42) and basal diet containing vitamin E at 70 g/ton feed (n=150). G2 and G3 birds were subjected to high stocking density (0.0279 m2/bird from day 1 to 21 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler performance; High stocking density; Oxidative stress; Vitamin E.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000400587

Effects of In-Ovo Rutin Injection to Fertile Japanese Quail (Coturnix Coturnix Japonica) Egg on Hatchability, Embryonic Death, Hatchling Weight, and Hatchling Liver Oxidative and Nitrosative Stress
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Genc,M; Kandemir,FM; Coban,O.
ABSTRACT This study was conducted with the aim of investigating the effects of the antioxidant rutin injected in fertilized quail eggs on incubation parameters and some hatchling liver biochemical parameters. The study was carried out with 6 groups including a control group and 5 different doses of rutin, and it involved 720 fresh Japanese quail (Coturnix coturnix japonica) eggs. It was observed that rutin dose did not affect the early embryo mortality, whereas intermediate and late embryo mortality rates were higher in all groups given rutin in comparison to the control group. The mean hatchability of fertile eggs and total eggs for the control, 0.25 mg, 0.50 mg, 0.75 mg, 1 mg and 1.5 mg groups were calculated as 82.06, 82.23, 64.43, 68.84, 44.08, 22.95 %...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hatchability; In-ovo injection; Oxidative stress; Nitrosative stress; Rutin.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100329

The Effects of Different Amount of Protein and Vitamin e Supplementation in Rations on Lipid and Antioxidant Metabolism of Broilers Exposed to Heat Stress
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Erol,HS; Imik,H; Gumus,R; Halici,M.
ABSTRACT Heat stress, causes economic losses and has negative effects on both broiler husbandry and animal welfare. Nutritional strategies are applied for minimizing the negative effects of heat stress. In the present study, at the finishing period (24-39 days of age) of heat stress, the effects of diet involving 21% and 19% proteins and vitamin E on lipid metabolism and antioxidant mechanism of action, aimed to be identified. This study was carried out in six groups as: HPC (24oC heat + 21% crude protein (CP)), HPS (34oC heat + 21% CP), LPC (24oC heat + 19% CP), LPS (34oC heat + 19% CP), HPSVE (34oC heat + 21% CP + Vitamin E) and LPSVE (34oC heat + 19% CP + vitamin E) groups. Superficial pectoral muscles (breast) and liver tissues were used for oxidative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heat stress; Broiler; Protein; Vitamin E; Oxidative stress.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000200289

Effect of dried extract of boldo (Peumus Boldus Mol.) on growth and oxidative tissue status of broiler chickens
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Fellenberg,MA; Delporte,C; Backhouse,N; Peña,I; Speisky,H.
The addition of antioxidants to broiler diets has been shown to enhance their antioxidant status. Since boldo (Peumus boldus Mol.) leaves contain highly antioxidant molecules, a dried extract of boldo (DEB) was added to broiler diets to improve "in vivo" antioxidant tissue status and to favor animal growth. A DEB standardized for antioxidant content was prepared and added to poultry diets at three different levels (low-DELB, medium-DEMB, and high-DEHB) for a period of 6 weeks. A single negative control (no added antioxidant) and one positive control (supplementation with 200 mg/kg vitamin E) were used. Plasma antioxidant capacity (PAC), thiol content (GHS), and basal and induced lipoperoxidation of liver, leg and breast tissues were determined in birds at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Boldo; Broiler; Oxidative stress.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2008000400009

Antioxidant supplementation to the exercising horse
Provedor de dados: Revista Brasileira de Zootecnia
Autores: Williams,Carey A.
Evidence of oxidative stress in horses has been described in reports dealing with intense and endurance exercise. Oxidative stress occurs when antioxidant systems are insufficient causing oxidation to potentially damage DNA, proteins, and lipids, and contribute to degenerative changes. Vitamin E is the most commonly supplemented antioxidant in horses and has been shown that horses in exercise conditioning may require higher intakes of vitamin E than recommended. Also in various species vitamin C potentiates the effects of vitamin E; however, under maintenance conditions horses have the ability to synthesize sufficient ascorbate, the demand increases as stress on the body is increased. Competitive endurance horses were estimated to consume 1.2 to 5-times...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Equine; Lipoic acid; Oxidative stress; Vitamin E.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001300017

The role of oxidative stress in small ruminants' health and production
Provedor de dados: Revista Brasileira de Zootecnia
Autores: Celi,Pietro.
This paper examines the role that oxidative stress plays in small ruminant medicine. We will examine how redox homeostasis is involved in some physiological functions and we will discuss the implications of the impairment of oxidative status on small ruminant health and production.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Health; Oxidative stress; Production; Ruminants.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001300038

Effect of different levels of alpha tocopherol on performance traits, serum antioxidant enzymes, and trace elements in Japanese quail ( Coturnix coturnix japonica ) under low ambient temperature
Provedor de dados: Revista Brasileira de Zootecnia
Autores: Shah,Assar Ali; Khan,Muhammad Saleem; Khan,Sarzamin; Ahmad,Nazir; Alhidary,Ibrahim A.; Khan,Rifat Ullah; Shao,Tao.
ABSTRACT This study was designed to find the effect of vitamin E supplementation on growth, serum antioxidant enzymes, and some trace elements in Japanese quail (Coturnix coturnix japonica) under low ambient temperature. A total of 180 day-old Japanese quails were randomly divided into four groups and provided with 0 (group A), 50 (group B), 100 (group C), and 150 IU/kg (group D) vitamin E (dl-α-tocopherol acetate) under an average temperature of 9±0.5 °C for an experimental period of 42 days. The result showed that feed intake per day, body weight, weight gain per day, and feed conversion ratio did not differ significantly between the groups. Serum concentrations of superoxide and glutathione peroxidase were significantly high in birds supplemented with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Trace minerals; Vitamin E.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016001000622

Recent advances in sow nutrition
Provedor de dados: Revista Brasileira de Zootecnia
Autores: Kim,Sung Woo.
Sows produce a larger number of fetuses than before and these fetuses possess genetic potentials to grow faster than before. Recent comparison shows that a porcine fetus is 40% heavier than 40 years ago. Thus the nutritional management of sows has been updated to reflect these genetic changes. Our recent study quantified nutritional needs for sows to support the growth of fetuses and mammary glands during gestation. Amino acid needs for fetal growth and mammary growth during late gestation (d 70 to farrowing) significantly greater than those needs during early gestation (until d 70). Considering these increases, daily requirement of true ileal digestible Lys for a primiparous sows increases from 7 g (until d 70) to 15 g (d 70 to farrowing). Required...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amino acids; Fetus; Gestation; Lactation; Oxidative stress; Sows.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001300033

Effect of adenosine concentration on quality of cooled ram semen
Provedor de dados: Revista Brasileira de Zootecnia
Autores: Bezerra,Andréia Santana; Nascimento,Thialla Emille Costa do; Castilho,Erick Fonseca de; Gonçalves,Natália Larissa Cunha; Silva,Sillas Matteus Batista Soares da; Cardoso,Aline Serrão; Souza,Heloíse Karine Rolim de; Rodrigues,Luiz Fernando de Souza.
ABSTRACT This study analyzed the adenosine concentration on quality of ram semen in different refrigerated storage times at 5 °C. The design was in blocks with repeated-measures factorial design. Five levels of adenosine and four refrigeration times were considered as fixed effects and the animal as random effect (block). Ram semen was diluted with Andromed® diluent (control) and increasing adenosine levels (0.5, 0.75, 1, and 1.5%). The samples were taken from refrigeration after 4, 8, 12, and 16 h and evaluated for progressive rectilinear sperm motility, sperm vigor, and membrane integrity (supravital test). Data were subjected to analysis of variance with Tukey's test. The regression analysis was conducted to evaluate behavior of adenosine levels. Factor...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nucleoside; Oxidative stress; Purinergic; Reproduction; Spermatozoon.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100606

Feeding systems and tocopherol level in the diet and their effects on the quality of lamb meat: a meta-analysis
Provedor de dados: Revista Brasileira de Zootecnia
Autores: Hampel,Viviane da Silva; Poli,Cesar Henrique Espírito Candal; Devincenzi,Thais; Pötter,Luciana.
ABSTRACT The objective of the present study was to evaluate, through a meta-analysis, the effects of feeding systems and tocopherol levels found in the diet of lambs on qualitative characteristics of their meat. A search of the computerised literature in Science Direct databases, PubMed, Scopus, and SciELO virtual library was carried out to select works that evaluated the quality of lamb meat. As a first requirement for inclusion in the meta-analysis, articles were selected with the keywords “tocopherol”, “meat”, and “lamb”. The variables extracted from the articles were classified according to the type of feeding system and tocopherol level in the diet of lambs. Production systems alter the qualitative characteristics of meat. Lambs raised exclusively on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Concentrated; Omega 3; Oxidative stress; Pasture.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100711

Effects of ascorbic acid on the germination and vigour of cowpea seeds under water stress
Provedor de dados: Revista Ciência Agronômica (RCA)
Autores: Nunes,Luma Rayane de Lima; Pinheiro,Paloma Rayane; Silva,João Batista da; Dutra,Alek Sandro.
ABSTRACT Water is one of the most compromising factors in the germination and initial growth of seedlings, where its restriction causes a reduction in the water potential of cells in addition to causing oxidative stress. Ascorbic acid (AsA) is known to protect organelles and cells against the accumulation of ROS. The aim of this work was to study the effects of ascorbic acid on the conditioning of cowpea seeds subjected to water stress. The seeds of the BRS Marataoã and Setentão genotypes were conditioned at a concentration of 0.0 (control), 0.25, 0.50, 0.75 and 1.00 mM AsA, and sown on paper rolls (Germitest®) moistened with mannitol solution at a potential of 0.0 (control), -0.3, -0.6, -0.9 and -1.2 MPa, and stored in a germination chamber (BOD) at 25...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vigna unguiculata L.; Oxidative stress; Drought.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000200407

Nitrogen source influences the antioxidative system of soybean plants under hypoxia and re-oxygenation
Provedor de dados: Scientia Agricola
Autores: Borella,Junior; Becker,Rafael; Lima,Milene Conceição; Oliveira,Denise dos Santos Colares de; Braga,Eugenia Jacira Bolacel; Oliveira,Ana Claudia Barneche de; Amarante,Luciano do.
ABSTRACT: In this work, we compared nitrate-supplied plants (non-nodulated) with non-nitrate-supplied plants (nodulated) under oxygen privation of root system (hypoxia) and re-oxygenation (post-hypoxia; recovery) in order to verify whether N sources affect the antioxidant system during oxidative stress caused by hypoxia and post-hypoxia conditions. Antioxidant enzymatic activities, ascorbate redox state, and reactive oxygen species (ROS) levels were analyzed in roots and leaves of two soybean genotypes, Fundacep 53 RR and BRS Macota at reproductive stage R2, during hypoxia and post-hypoxia in an experiment carried out in a hydroponic system. The antioxidant system was strongly induced in roots of nitrate-supplied plants of both genotypes, with high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Waterlogging; Oxidative stress; Antioxidative enzymes.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019000100051

Stomatal behavior and components of the antioxidative system in coffee plants under water stress
Provedor de dados: Scientia Agricola
Autores: Deuner,Sidnei; Alves,José Donizeti; Zanandrea,Ilisandra; Goulart,Patrícia de Fátima Pereira; Silveira,Neidiquele Maria; Henrique,Paôla de Castro; Mesquita,Alessandro Carlos.
Coffee (Coffea arabica) plants show a positive relationship between stomatal closure and formation and accumulation of H2O2. However, for coffee plants under water restriction such relationship has never been studied. The objective of the present study was evaluate the stomatal movement and the antioxidant capacity of coffee seedlings under different water regimes. Eight months old coffee seedlings of cv. Catuaí IAC 99 were submitted to field capacity, gradual and total suspension of irrigation during a period of 21 days. Evaluations of leaf water potential (Ψw) were performed in the beginning of the morning, and stomatal resistance, transpiration rate and vapor pressure deficit were determined at 10 am and 5 pm. All biochemical and enzymatic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea arabica; Oxidative stress; Antioxidants; Water stress.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000100012

Antioxidant system of ginseng under stress by cadmium
Provedor de dados: Scientia Agricola
Autores: Marques,Teresa Cristina Lara Lanza de Sá e Melo; Soares,Angela Maria.
Toxic levels of Cd can cause protein denaturation and oxidative stress, which result in membrane damage, enzimatic activity changes and other metabolic damage. Some plants may show alteration in the activity of their antioxidant enzymes as a heavy metal tolerance mechanism. This study aims at evaluating the role of enzymes of the antioxidant system in adaptive responses of the accumulator P. glomerata species to levels of cadmium (Cd). Plants were cultivated in nutrient solutions containing concentrations of Cd in the form of CdSO4 (0, 45 and 90 µmol L-1), for 20 d. Cd concentrations and yields of root and shoot dry matter were determined at the end of the experiment. Malondialdehyde (MDA) production and the activities of antioxidant enzymes were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant enzymes; Oxidative stress; Tolerance; Cadmium toxicity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400014

Oxidative damage and photosynthetic impairment in tropical rice cultivars upon exposure to excess iron
Provedor de dados: Scientia Agricola
Autores: Pinto,Samuel de Souza; Souza,Anna Elisa de; Oliva,Marco Antonio; Pereira,Eduardo Gusmão.
ABSTRACT Iron plays a pivotal role in the redox reactions of photosynthesis and metabolic processes such as chlorophyll synthesis. Iron availability in waterlogged soils can reach toxic levels and promote oxidative stress. Fe toxicity is the most concerning of stresses for rice in many lowland environments around the world and may cause severe impairments in rice photosynthesis. This study aimed to investigate the extension of oxidative stress after excess Fe exposure and its effects on the photosynthesis of rice cultivars with differential sensitivity. Three Brazilian rice cultivars (EPAGRI 107, BRSMG SELETA and BR IRGA 409) were grown in Hoagland nutrient solution (pH 4.0) with two Fe-EDTA doses corresponding to excess Fe (7 mM) and control (0.009 mM)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TBARS; Iron toxicity; Light energy dissipation; Oxidative stress; Photosynthesis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000300217

Antioxidant and hepatoprotective effects of ethanolic and ethyl acetate stem bark extracts of Copaifera multijuga (Fabaceae) in mice
Provedor de dados: Acta Amazonica
Autores: PEREIRA,Débora Linsbinski; CUNHA,Ana Paula Simões DA; CARDOSO,Cássia Regina Primila; ROCHA,Cláudia Quintino DA; VILEGAS,Wagner; SINHORIN,Adilson Paulo; SINHORIN,Valéria Dornelles Gindri.
ABSTRACT The properties of oil-resin of copaiba, Copaifera multijuga are commonly mentioned in the literature, but there are few studies on extracts from its stem bark. We evaluated the antioxidant effects of ethanolic (EE) and ethyl acetate (EA) crude stem bark extracts from copaiba and compared them to rutin in a paracetamol (PCM)-induced oxidative stress model in mice. All test comparisons differed significantly. Hepatic catalase (CAT) and glutathione-S-transferase (GST) activity decreased in the PCM group, and there was an increase of protein carbonyls in the liver, kidney and brain. However, the protein carbonyls decreased in the liver for the PCM + EE group, in the kidneys for the PCM + EA and PCM + Rutin groups, and in the brain for all treatments....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Acute intoxication; Acetaminophen; Copaiba.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672018000400347

First phytochemical and biological study of the ethanolic extract from leaves of Capirona decorticans (Rubiaceae)
Provedor de dados: Acta Amazonica
Autores: BARBOSA,Fernando Gomes; SUGUI,Marina Mariko; SINHORIN,Valéria Dornelles Gindri; BICUDO,Rogério de Campos; MOURA,Fernando Rafael de; SINHORIN,Adilson Paulo.
ABSTRACT Capirona decorticans (Rubiaceae) is popularly used to treat warts, wounds, mycoses and scabies, and is also a component of the Ayahuasca tea. Despite its popular use, the phytochemical and pharmacological research on this species is limited. Therefore, this work quantified phenolic compounds in the ethanolic extract (EE) and hydromethanolic fraction (FM) (406, 293 mgEAG g-1, respectively) from leaves of C. decorticans. We identified flavonoids by LC-MS/MS-MMR-ESI (apigenin, rutin, luteolin, miricetin, quercetin, quercetin-3-β-D-glucoside, quercetrin), and evaluated oxidative stress and mutagenic/antimutagenic effect of EE and FM through an in vivo experiment using Swiss mice and cyclophosphamide (CP) as an inducer of DNA damage and oxidative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Cyclophosphamide; Genotoxicity; Phenolic compounds.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672018000400338

Evaluation of the antioxidant potential of Copaifera multijuga in Ehrlich tumor-bearing mice
Provedor de dados: Acta Amazonica
Autores: CUNHA,Ana Paula Simões da; BALDISSERA,Luana; PEREIRA,Débora Linsbinski; ALBIERO,Lucineia Reuse; CASTOLDI,Lindsey; SINHORIN,Adilson Paulo; SINHORIN,Valéria Dornelles Gindri.
ABSTRACT Copaifera multijuga, commonly known as copaiba, is popularly used in the form of tea for various conditions due to the presence of antioxidant substances in its composition, which protect cells against damage caused by free radicals. Its oleoresin is also used as an anti-inflammatory and antitumoral agent. The present study investigated the antioxidant effect of the ethanolic extract of copaiba stem bark on Swiss mice inoculated with solid Ehrlich tumors. Mice were inoculated subcutaneously with 1x106 Ehrlich’s tumor cells and treated via gavage with ethanolic extract of copaiba for thirty days, with doses varying between 100 and 200 mg kg-1. Biochemical analyses of enzymatic antioxidants [superoxide dismutase (SOD), catalase (CAT),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solid tumor; Oxidative stress; Free radicals; Copaiba; Antineoplasic.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000100041

RESPONSE TO in vitro SALT STRESS IN SUGARCANE IS CONDITIONED BY CONCENTRATION AND CONDITION OF EXPOSURE TO NaCI
Provedor de dados: Acta Biológica Colombiana
Autores: CAVALCANTE GRANJA,Manuela Maria; LACERDA E MEDEIROS,Maria Jaislanny; MEDEIROS DE ANDRADE SILVA,Marina; CAMARA,Terezinha; WILLADINO,Lilia; ULISSES,Claudia.
ABSTRACT Salinity is one of the major environmental stress factors that affect crop productivity, as well as interfering with plant growth and development, resulting in reduced production quality. Given this, we highlight the importance of research in response to plants subjected to salt stress in order to assess the physiological and biochemical behavior of genotypes, with the objective of selecting the more tolerant. One way to ensure the uniformity of the response of the plants is through in vitro cultivation, which allows control of the cultivation conditions. Therefore, the objective of this work was to evaluate the response of two commercial varieties of sugar cane exposed to saline stress in different conditions (gradual and abrupt). Two varieties...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant enzymes; In vitro culture; Oxidative stress; Salinity.
Ano: 2018
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2018000100030

EFFECT OF EXOGENOUS PROLINE IN TWO SUGARCANE GENOTYPES GROWN IN VITRO UNDER SALT STRESS
Provedor de dados: Acta Biológica Colombiana
Autores: L. MEDEIROS,Maria Jaislanny; Medeiros De A. SILVA,Marina; C. GRANJA,Manuela Maria; De SOUZA E SILVA JÚNIOR,Gilberto; CAMARA,Terezinha; WILLADINO,Lilia.
Sugarcane (Saccharum officinarum L.) production is influenced by various abiotic stresses, including salt stress. Salinity can induce oxidative stress, which in turn damages biomolecules and cellular structures. However, such damage can be avoided or minimized by the enzymatic and non-enzymatic defense systems of plants. The aim of the study was to determine the effect of exogenous proline on Na+ and K+ content and on the activity of catalase, ascorbate peroxidase and peroxidase in two sugarcane genotypes (RB931011 and RB872552) grown in vitro under salt stress. The plants were grown for 20 days with or without 100 mM NaCl and exposed to 20 mM proline for varying period of time. The data were subjected to ANOVA factorial (two genotypes and five...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant enzymes; Oxidative stress; Saccharum officinarum L.
Ano: 2015
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000200006

Algicidal effects of aqueous leaf extracts of neem (Azadirachta indica) on Scenedesmus quadricauda (Turp.) de Brébission
Provedor de dados: Acta Botanica Brasilica
Autores: Chia,Mathias Ahii; Akinsanmi,Jamiu Taiwo; Tanimu,Yahuza; Ladan,Zakari.
The application of synthetic algaecides for the control of algae produces by-products that are sometimes toxic to the environment. There is a need for natural and cheap alternatives to synthetic algaecides. In the present study, we investigated the potential of aqueous crude extract of Azadirachta indica to inhibit the growth of Scenedesmus quadricauda. Phytochemical screening of the extract revealed the presence of groups of bioactive compounds that are capable of inhibiting microalgal growth. Chlorophyll a concentration, dry weight production and cell density of microalga decreased with increasing crude extract concentration. After three days of exposure, the 1000 mg/L extract concentration resulted in complete growth inhibition and cell lysis....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Algaecide; Growth inhibition; Microalgae; Neem; Oxidative stress; Phenotypic plasticity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062016000100001

Structural changes in Psidium guajava 'Paluma' leaves exposed to tropospheric ozone
Provedor de dados: Acta Botanica Brasilica
Autores: Tresmondi,Fernanda; Alves,Edenise Segala.
Psidium guajava 'Paluma' has being tested as an ozone (O3) bioindicator and responds with pigmentation between the veins on the adaxial surface, due to the accumulation of phenolic compounds. These compounds act as non-enzymatic antioxidants that neutralize reactive oxygen species (ROS), formed from O3. This study aimed to evaluate the leaf structure of plants with and without visible symptoms and to establish these symptoms at the cellular level. Beside this we also aimed to detect structural changes that can minimize the effects of the O3 on the plant. The accumulation of phenolic substances, stomatal density and structural changes in P. guajava 'Paluma' leaf tissues exposed during the four seasons of the year were evaluated. The study was conducted at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioindicator; Phenols; Leaf anatomy; Oxidative stress; Stomatal density.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062011000300005

Ethnobotany and antioxidant evaluation of commercialized medicinal plants from the Brazilian Pampa
Provedor de dados: Acta Botanica Brasilica
Autores: Teixeira,Marianne Pinheiro; Cruz,Litiele; Franco,Jeferson Luis; Vieira,Renara Bittencourt; Stefenon,Valdir Marcos.
Despite the importance of medicinal plants for the healthcare of local people, the knowledge about medicinal species used in the Pampa biome has been neglected over the years. In this study, an ethnobotanical survey was employed aiming to characterize the species richness and diversity of commercialized medicinal plant species in five cities within the Brazilian Pampa. Additionally, among the listed plants, ten species were selected for in vitro testing of their potential antioxidant activity. A total of 56 plant species belonging to 33 botanical families were listed by the 115 interviewees. No significant difference in commercialized medicinal plant species, and very similar species richness was observed among the cities, indicating that the local...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethnobiology; Ethnopharmacology; Gaúcho; Oxidative stress; Pampa biome.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062016000100047

Salt stress: antioxidant activity as a physiological adaptation of onion cultivars
Provedor de dados: Acta Botanica Brasilica
Autores: Corrêa,Natália Silveira; Bandeira,Juliana de Magalhães; Marini,Patrícia; Borba,Isabel Cristina Gouvea de; Lopes,Nei Fernandes; Moraes,Dario Munt de.
The germination and development of several plant species can be limited when those plants are grown in soils with high salinity, which reduces seedling viability and vigor, as well as activating the antioxidant defense system. The aim of this study was to evaluate germination, initial growth and activity of antioxidant enzymes (superoxide dismutase, catalase and ascorbate peroxidase) in seedlings of three onion cultivars (Madrugada, Fepagro 27 and Petroline) exposed to different concentrations of NaCl (0, 40, 80, 120 and 160 mM). Seedlings were evaluated for viability, vigor and antioxidant enzyme activity. The experimental procedures were completely randomized in 3 × 5 factorial design, with each treatment performed in triplicate, at a significance level...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; Growing; Oxidative stress; Germination; NaCl.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000200013

Role of glutathione in tolerance to arsenite in Salvinia molesta, an aquatic fern
Provedor de dados: Acta Botanica Brasilica
Autores: Silva,Adinan Alves da; Oliveira,Juraci Alves de; Campos,Fernanda Vidal de; Ribeiro,Cleberson; Farnese,Fernanda dos Santos.
ABSTRACT In many plant species, tolerance to toxic metals is highly dependent on glutathione, an essential metabolite for cellular detoxification. We evaluated the responses of glutathione metabolism to arsenite (AsIII) in Salvinia molesta, an aquatic fern that has unexplored phytoremediation potential. Plants were exposed to different AsIII concentrations in nutrient solution for 24 h. AsIII caused cell membrane damage to submerged leaves, indicating oxidative stress. There was an increase in the glutathione content and ϒ-glutamylcysteine synthetase enzyme activity in the submerged and floating leaves. The glutathione peroxidase and glutathione sulfotransferase enzymes also showed increased activity in both plant parts, whereas glutathione reductase only...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arsenic; Glutathione; Macrophytes; Oxidative stress; Phytoremediation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000400657

Pretreatment of forage legumes under moderate salinity with exogenous salicylic acid or spermidine
Provedor de dados: Acta Scientiarum: Agronomy
Autores: Pizolato Neto, Antonio; Alves, Rita de Cássia; Camargos, Ayza Eugênio Viana; Gratão, Priscila Lupino; Carregari, Sônia Maria Raymundo; Zingaretti, Sonia Marli; Santos, Durvalina Maria Mathias Dos.
The present study aims to determine whether exogenous salicylic acid (SA) or spermidine (Spd) has any protective effect against salt stress. Seeds were subjected to 0, 20, 40, and 60 mM NaCl with or without salicylic acid or spermidine (0.5 mM) for 10 days. The evaluated variables were germination rate, shoot and root dry masses, glycine betaine content, lipid peroxidation, and the activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX). The data were subjected to Tukey’s test (p ≤ 0.05). There was a growth increase, especially in plant shoots. The reduction in lipid peroxidation, as indicated by lower malondialdehyde (MDA) levels, can be explained by an increase in antioxidant activity when SA and Spd were added. When...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cajanus cajan; Dolichos lablab; Salt stress; Glycine betaine; Oxidative stress; Growth. Cajanus cajan; Dolichos lablab; Salt stress; Glycine betaine; Oxidative stress; Growth. Fisiologia Vegetal.
Ano: 2020
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42809

The compensatory effect of glutathione on alleviating salinity–induced modulations in growth and biochemical traits in maize irrigated with diluted seawater
Provedor de dados: Agricultural Engineering International : The CIGR e-journal
Autores: El-Shahawy, Tarek Abd El-Ghafar.
Salinity stress has recently received much attention as an object worthy of research and interest. It is a great challenge for the future global agricultural production that aspires to a large-scale conversion of raw seawater to irrigation use. Our study aims to investigate the antioxidant and free radical scavenging effect of glutathione (GSH) that would enhance maize tolerance to the destructive effect of salinity. A greenhouse trail was conducted in this context during the summer season of 2015 using two salinity (Mediterranean seawater: 3000 and 6000 ppm) and GSH (100 and 200 pm) levels. Tap water was used as a control. Individually, saline water acted in a distinctly different manner than GSH. Irrigation with diluted seawater caused morphological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant physiology; Environmental stresses Antioxidants; Oxidative stress; Reactive oxygen species (ROS); Salinity; Tolerance; Zea mays.
Ano: 2018
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/4393

Temporal dynamics of the response to Al stress in Eucalyptus grandis × Eucalyptus camaldulensis
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: Alcântara,Berenice K. de; Pizzaia,Daniel; Piotto,Fernando A.; Borgo,Lucélia; Brondani,Gilvano E.; Azevedo,Ricardo A.
Lipid peroxidation and root elongation of Eucalyptus grandis × Eucalyptus camaldulensis were studied under stress conditions in response to aluminum (Al), a metal known to limit agricultural productivity in acidic soils primarily due to reduced root elongation. In Brazil, the Grancam 1277 hybrid (E. grandis × E. camaldulensis) has been planted in the "Cerrado", a region of the country with a wide occurrence of acidic soils. The present study demonstrated that the hybrid exhibited root growth reduction and increased levels of lipid peroxidation after 24h of treatment with 100 µM of Al, which was followed by a reduction in lipid peroxidation levels and the recovery of root elongation after 48h of Al exposure, suggesting a rapid response to the early...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aluminum; Eucalypt; Lipid peroxidation; Oxidative stress; Root growth.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000201063

Reduction of the DNA damages, Hepatoprotective Effect and Antioxidant Potential of the Coconut Water, ascorbic and Caffeic Acids in Oxidative Stress Mediated by Ethanol
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: BISPO,VANDERSON S.; DANTAS,LUCAS S.; CHAVES FILHO,ADRIANO B.; PINTO,ISABELLA F.D.; SILVA,RAILMARA P. DA; OTSUKA,FELIPE A.M.; SANTOS,RODRIGO B.; SANTOS,ALINE C.; TRINDADE,DANIELLE J.; MATOS,HUMBERTO R.
ABSTRACT Hepatic disorders such as steatosis and alcoholic steatohepatitis are common diseases that affect thousands of people around the globe. This study aims to identify the main phenol compounds using a new HPLC-ESI+-MS/MS method, to evaluate some oxidative stress parameters and the hepatoprotective action of green dwarf coconut water, caffeic and ascorbic acids on the liver and serum of rats treated with ethanol. The results showed five polyphenols in the lyophilized coconut water spiked with standards: chlorogenic acid (0.18 µM), caffeic acid (1.1 µM), methyl caffeate (0.03 µM), quercetin (0.08 µM) and ferulic acid (0.02 µM) isomers. In the animals, the activity of the serum γ-glutamyltranspeptidase (γ-GT) was reduced to 1.8 I.U/L in the coconut...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coconut water; Ethanol; Oxidative stress; Phenolic compounds.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301095

Relationship between testicular lesion and hormone levels in male rats infected with Trypanosoma evansi
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: FACCIO,LUCIANA; SILVA,ALEKSANDRO S. DA; TONIN,ALEXANDRE A.; OBERHERR,LUCAS; GRESSLER,LUCAS T.; OLIVEIRA,CAMILA B.; OLIVEIRA,DIONATAN T.; SANGOI,MANUELA B.; MORESCO,RAFAEL N.; SAMARA,YASMIN N.; VEIGA,MARCELO; DUARTE,MARTA M.M.F.; MONTEIRO,SILVIA G.
The aim of this study was to evaluate the relationship between testicular lesions and hormone levels in rats experimentally infected with Trypanosoma evansi. For that, the measurement of reproductive hormones, histopathology and biomarkers of cellular injury were carried out in twenty-four animals, which were divided into two groups with 12 animals each. Group A was the negative control, or uninfected, while group B was composed by animals infected with T. evansi. Both groups were divided again into two other subgroups (n=6), from which serum and testicular fragments were collected on days 5 (A1 and B1) and 15 (A2 and B2) post-infection (PI). The morphological analysis showed increased alterations of head and tail of sperm in infected rats when compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protozoa; Wistar rats; Testicular lesion; Reproduction; Oxidative stress.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301537

Mitochondrial DNA damage associated with lipid peroxidation of the mitochondrial membrane induced by Fe2+-citrate
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: Almeida,Andréa M.; Bertoncini,Clélia R.A.; Borecký,Jiri; Souza-Pinto,Nadja C.; Vercesi,Aníbal E.
Iron imbalance/accumulation has been implicated in oxidative injury associated with many degenerative diseases such as hereditary hemochromatosis, beta-thalassemia, and Friedreich's ataxia. Mitochondria are particularly sensitive to iron-induced oxidative stress - high loads of iron cause extensive lipid peroxidation and membrane permeabilization in isolated mitochondria. Here we detected and characterized mitochondrial DNA damage in isolated rat liver mitochondria exposed to a Fe2+-citrate complex, a small molecular weight complex. Intense DNA fragmentation was induced after the incubation of mitochondria with the iron complex. The detection of 3' phosphoglycolate ends at the mtDNA strand breaks by a 32P-postlabeling assay, suggested the involvement of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Iron; Lipid peroxidation; Mitochondria; MtDNA; Oxidative stress.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000300010

Vitamin B6 reduces oxidative stress in lungs and liver in experimental sepsis
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: GIUSTINA,AMANDA D.; DANIELSKI,LUCINÉIA G.; NOVOCHADLO,MICHELE M.; GOLDIM,MARIANA P.S.; JOAQUIM,LARISSA; METZKER,KIUANNE L.L.; CARLI,RAQUEL JACONI DE; DENICOL,TAIS; CIDREIRA,THAINA; VIEIRA,THAYNAN; PETRONILHO,FABRICIA.
Abstract Abstract: Sepsis is a life-threatening organ dysfunction induced by a disrupted host response to infecting pathogens. Inflammation and oxidative stress are intrinsically related to sepsis progression and organ failure. Vitamin B6 is an important cellular cofactor for metabolic processes and has anti-inflammatory and antioxidant properties. We aimed at evaluating the effect of vit B6 on inflammation and oxidative stress markers in the liver and lung of rats subjected to a relevant animal model of polymicrobial sepsis. Adult male Wistar rats were submitted to ﻿cecal ligation and perforation model and immediately after sepsis induction, vit B6 was administered as a single dose (600 mg/kg, subcutaneous). Twenty-four hours later, the lung and liver...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sepsis; Vitamin B6; Oxidative stress; Antioxidant.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700627

Neurotrophins, cytokines, oxidative parameters and funcionality in Progressive Muscular Dystrophies
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: COMIM,CLARISSA M.; MATHIA,GISIANE B.; HOEPERS,ANDREZA; TUON,LISIANE; KAPCZINSKI,FLÁVIO; DAL-PIZZOL,FELIPE; QUEVEDO,JOÃO; ROSA,MARIA I.
We investigated the levels of brain derived-neurotrophic factor (BDNF), cytokines and oxidative parameters in serum and tried to correlate them with the age and functionality of patients with Progressive Muscle Dystrophies (PMD). The patients were separated into six groups (case and controls pared by age and gender), as follows: Duchenne Muscular Dystrophy (DMD); Steinert Myotonic Dystrophy (SMD); and Limb-girdle Muscular Dystrophy type-2A (LGMD2A). DMD patients (±17.9 years old) had a decrease of functionality, an increase in the IL-1β and TNF-α levels and a decrease of IL-10 levels and superoxide dismutase activity in serum. SMD patients (±25.8 years old) had a decrease of BDNF and IL-10 levels and superoxide dismutase activity and an increase of IL-1β...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Progressive Muscular Dystrophies; BNDF; Cytokines; Oxidative stress.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000401809

Biological functions of selenium and its potential influence on Parkinson's disease
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: ELLWANGER,JOEL H.; FRANKE,SILVIA I.R.; BORDIN,DIANA L.; PRÁ,DANIEL; HENRIQUES,JOÃO A.P.
ABSTRACT Parkinson's disease is characterized by the death of dopaminergic neurons, mainly in the substantia nigra, and causes serious locomotor dysfunctions. It is likely that the oxidative damage to cellular biomolecules is among the leading causes of neurodegeneration that occurs in the disease. Selenium is an essential mineral for proper functioning of the brain, and mainly due to its antioxidant activity, it is possible to exert a special role in the prevention and in the nutritional management of Parkinson's disease. Currently, few researchers have investigated the effects of selenium on Parkinson´s disease. However, it is known that very high or very low body levels of selenium can (possibly) contribute to the pathogenesis of Parkinson's disease,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurodegeneration; Nutrition; Oxidative stress; Parkinson's disease; Selenium.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501655

Zingiber officinale formulation reduces hepatic injury and weight gain in rats fed an unhealthy diet
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: LEAL,DALILA T.; FONTES,GLEIDE G.; VILLA,JULIA K.D.; FREITAS,RODRIGO B.; CAMPOS,MATEUS G.; CARVALHO,CAMILO A.; PIZZIOLO,VIRGINIA R.; DIAZ,MARISA A.N.
Abstract: This study investigated the ability of formulation containing Zingiber officinale (ginger) to reverse health changes promoted by unhealthy diet in Wistar rats. Five compounds from the gingerol family and three from the shogaol family were identified in the chromatographic analyzes of the extract. The animals were fed a combination of unhealthy foods, the cafeteria diet, which promoted increases in body weight, hepatocyte nucleus area, total hepatocyte area and liver fat accumulation, as well as reduced hepatic glutathione S-transferase concentration, compared to the control group, which received commercial chow. The treatment with ginger improved all these results, highlighting the reduction of 10% of body weight and 66% of the total area of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cafeteria diet; Gingerol; Nonalcoholic fatty liver disease; Oxidative stress; Shogaol; Zingiber officinale.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700801

The neurotoxic effects of vitamin A and retinoids
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: OLIVEIRA,MARCOS ROBERTO DE.
Vitamin A (retinol) and its congeners - the retinoids - participate in a panoply of biological events, as for instance cell differentiation, proliferation, survival, and death, necessary to maintain tissue homeostasis. Furthermore, such molecules may be applied as therapeutic agents in the case of some diseases, including dermatological disturbances, immunodeficiency, and cancer (mainly leukemia). In spite of this, there is a growing body of evidences showing that vitamin A doses exceeding the nutritional requirements may lead to negative consequences, including bioenergetics state dysfunction, redox impairment, altered cellular signaling, and cell death or proliferation, depending on the cell type. Neurotoxicity has long been demonstrated as a possible...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mitochondrial dysfunction; Neurotoxicity; Oxidative stress; Retinoids; Vitamin A.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000301361

Biochemical and histological characterization of tomato mutants
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: Monteiro,Carolina C.; Rolão,Milca B.; Franco,Mônica R.; Peters,Leila P.; Cia,Mariana C.; Capaldi,Flávia R.; Carvalho,Rogério F.; Gratão,Priscila L.; Rossi,Mônica L.; Martinelli,Adriana P.; Peres,Lázaro E.P.; Azevedo,Ricardo A.
Biochemical responses inherent to antioxidant systems as well morphological and anatomical properties of photomorphogenic, hormonal and developmental tomato mutants were investigated. Compared to the non-mutant Micro-Tom (MT), we observed that the malondialdehyde (MDA) content was enhanced in the diageotropica (dgt) and lutescent (l) mutants, whilst the highest levels of hydrogen peroxide (H2O2) were observed in high pigment 1 (hp1) and aurea (au) mutants. The analyses of antioxidant enzymes revealed that all mutants exhibited reduced catalase (CAT) activity when compared to MT. Guaiacol peroxidase (GPOX) was enhanced in both sitiens (sit) and notabilis (not) mutants, whereas in not mutant there was an increase in ascorbate peroxidase (APX). Based on PAGE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant enzymes; Leaf and root anatomy; Oxidative stress; Solanum lycopersicum.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200025

Low dissolved oxygen levels increase stress in piava (Megaleporinus obtusidens): iono-regulatory, metabolic and oxidative responses
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: COPATTI,CARLOS E.; BOLNER,KEIDI C.S.; LONDERO,ÉRIKA P.; ROSSO,FELIPE L. DE; PAVANATO,MARIA A.; BALDISSEROTTO,BERNARDO.
Abstract: The aquatic environment presents daily and/or seasonal variations in dissolved oxygen (DO) levels. Piava faces different DO levels in the water due to its distributional characteristics. The goal of this study was to describe the effects of low DO levels on plasma ion, biochemical and oxidative variables in piava juveniles. Fish were exposed to different DO levels, including 1.0, 2.0, 3.0, 4.0 and 5.0 mg L-1 of DO for 96 h, after which blood and tissue samples (liver, kidney, gill and muscle) were collected. The decrease in DO levels decreased plasma Na+, Cl-, K+ and NH3 levels as well as protein and glycogen levels in the liver, kidney and muscle; increased Na+/K+-ATPase activity in the gills and kidney as well as glucose and ammonia levels in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Iono-regulatory; Glucose; Lactate; Na+; K+-ATPase activity; Oxidative stress.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500858

In vitro effect of globotriaosylceramide on electron transport chain complexes and redox parameters
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: ALVARIZ,RAFAELA M.; MOREIRA,ISABEL T.D.S.; CURY,GABRIELA K.; VARGAS,CARMEN R.; BARSCHAK,ALETHÉA G.
Abstract: Fabry disease (FD) is an X-linked inherited disease and occurs due to mutations in GLA gene that encodes the α-galactosidase enzyme. Consequently, there is an accumulation of enzyme substrates, namely globotriaosylceramide (GB3). FD is a multisystemic disease, caused by storage of GB3 in vascular endothelia, with significant renal, cardiac and vascular involvement. The aim of this work was to evaluate the in vitro effect of GB3 on electron transport chain complexes (ETC) and redox parameters. Biochemical biomarkers were determined in homogenates of cerebral cortex, kidneys and liver of Wistar rats in the presence or absence of GB3 at concentrations of 3, 6, 9 and 12 mg/L. We found that GB3 caused an increase of ETC complexes II and IV activities,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electron transport chain; Fabry disease; Globotriaosylceramide; GB3; Oxidative stress; Reactive species.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300710

In vitro antioxidant capacity of tea of Echinodorus grandiforus, “leather hat,” in Wistar rat liver
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: LUNARDI,RAFAELA F.; WOHLENBERG,MARIANE; MEDEIROS,NIARA; AGOSTINI,FABIANE; FUNCHAL,CLÁUDIA; DANI,CAROLINE.
Oxidative stress has been considered as one of the factors responsible for hepatic diseases, which sometimes require new ways of treatment. The present study aimed to evaluate the in vitro antioxidant capacity of the tea of Echinodorus grandiforus (“leather hat” plant) in rat liver. Different preparations of tea were evaluated for phenolic composition, antioxidant activity by DPPH assay and ability to inhibit lipid peroxidation induced by copper sulfate. The antioxidant activity was assessed in liver tissue treated with sodium azide in the presence or absence of tea by assays for lipid peroxidation (TBARS), protein oxidation (carbonyl) and the antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD). The results show that different preparations of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Phenolic compounds; Reactive species; Sodium azide.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301451

Antioxidant effect of simvastatin throught oxidative imbalance caused by lisdexamfetamine dimesylate
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: Eger,Guilherme A.; Ferreira,Vinícius V.; Batista,Camila R.; Bonde,Henrique; Lima,Daniela D. de; Wyse,Angela T.S.; Cruz,Júlia N. da; Rodrigues,André F.; Magro,Débora D. Dal; Cruz,José G.P. da.
The present study aims to directly investigate the behavioral and antioxidant effects of simvastatin in a model of bipolar mania induced by lisdexamfetamine dimesylate. Wistar rats were treated for 30 days with simvastatin. On the 24th day after the start of treatment, each rat was administered lisdexamfetamine dimesylate for 7 days. The results suggest that simvastatin combined with lisdexamfetamine dimesylate induced a significant increased locomotion and lisdexamfetamine dimesylate administration causes an oxidative imbalance determined by an increment in lipid peroxidation, protein oxidation and alterations in the activities of antioxidant enzymes in brain areas; moreover, in the presence of simvastatin, most of these effects were prevented. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amphetamine; Lisdexamfetamine dimesylate; Oxidative stress; Simvastatin.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100335

Regular exercise, overweight/obesity and sedentary lifestyle cause adaptive changes in thiol–disulfide homeostasis
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: GOL,MEHMET; ÖZKAYA,BEYTULLAH; YILDIRIM,CANER; BAL,RAMAZAN.
Abstract: Dynamic thiol–disulfide homeostasis is considered to have critical roles in maintenance of physiological functioning. We aimed to reveal whether there is any specific aberration in thiol–disulfide homeostasis in three distinct categories of individuals, including those who 1) exercise regularly (fitness group), 2) have a sedentary lifestyle (sedentary group) and 3) are overweight or obese (overweight/obese group). 72 male individuals were included in the study, 21 of whom were in fitness group, 28 of whom were overweight or obese and 23 of whom had a sedentary lifestyle. Plasma native thiol (-SH) and total thiol [(-SH) + (-S-S-)] levels were quantitatively determined. Total thiol levels in sedentary group were significantly lower than those in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise; Obesity; Oxidative stress; Thiol-disulfide homeostasis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300801

Endothelial, renal and hepatic variables in wistar rats treated with Vancomycin
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: BRUNIERA,FELIPE R.; FERREIRA,FELIPE M.; SAVIOLI,LUIZ R.M.; BACCI,MARCELO R.; FEDER,DAVID; PEREIRA,EDIMAR C.; PEDREIRA,MAVILDE L.G.; PETERLINI,MARIA A.S.; PERAZZO,FÁBIO F.; AZZALIS,LIGIA A.; ROSA,PAULO C.P.; JUNQUEIRA,VIRGINIA B.C.; SATO,MONICA A.; FONSECA,FERNANDO L.A.
Vancomycin (VCM) is indicated in combat against Gram-positive infections, but it is not considered a first-choice drug because of its adverse effects. It is believed that oxidative stress is the primary mechanism of endothelial injury and the consequent VCM toxicity, which varies from phlebitis to nephrotoxicity. Moreover, dose recommendations, dilution, rates and types of infusion are still controversial. The aim of this study was to determine the effect of different VCM dilutions in endothelial, liver and kidney injuries by biochemical parameters and histopathological analysis. Wistar rats were randomly divided into six groups and subjected to femoral vein cannulation for drug administration. Control groups received 0.9 ml of saline and the others...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vancomycin; Acute kidney injury; Oxidative stress; Hepatotoxicity; Endothelial injury.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401963

Effects of Aliskiren, an RAAS inhibitor, on a carrageenan-induced pleurisy model of rats
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: BAYIR,YASIN; UN,HARUN; CADIRCI,ELIF; AKPINAR,EROL; DIYARBAKIR,BUSRA; CALIK,ILKNUR; HALICI,ZEKAI.
Abstract Our aim is to investigate the potentially preventive effects of Aliskiren in a carrageenan-induced lung pleurisy model and to compare the standard anti-inflammatory agents, indomethacin and dexamethasone. The pleurisy model was induced through the injection of carrageenan (0.2 ml-%2) into the pleural cavity. After the experiment, serum and lung tissues were collected and biochemical, molecular and pathological examinations were performed. In our study, pleural inflammation decreased superoxide dismutase activity and the glutathione level and increased the malondialdehyde level in the lung of rats, while Aliskiren increased the superoxide dismutase activity and glutathione level and decreased the malondialdehyde level. In addition,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aliskiren; Cytokines; Lung injury; Oxidative stress; Pleurisy; Rat.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100801

The pentose phosphate pathway in Trypanosoma cruzi: a potential target for the chemotherapy of Chagas disease
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: Igoillo-Esteve,Mariana; Maugeri,Dante; Stern,Ana L.; Beluardi,Paula; Cazzulo,Juan J.
Trypanosoma cruzi is highly sensitive to oxidative stress caused by reactive oxygen species. Trypanothione, the parasite's major protection against oxidative stress, is kept reduced by trypanothione reductase, using NADPH; the major source of the reduced coenzyme seems to be the pentose phosphate pathway. Its seven enzymes are present in the four major stages in the parasite's biological cycle; we have cloned and expressed them in Escherichia coli as active proteins. Glucose 6-phosphate dehydrogenase, which controls glucose flux through the pathway by its response to the NADP/NADPH ratio, is encoded by a number of genes per haploid genome, and is induced up to 46-fold by hydrogen peroxide in metacyclic trypomastigotes. The genes encoding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Chagas disease; Pentose phosphate pathway; Oxidative stress; NADPH generation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000400007

Early fragmentation of polyester urethane sheet neither causes persistent oxidative stress nor alters the outcome of normal tissue healing in rat skin
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: SGROTT,SANDRO M.; NEVES,RODRIGO D.; D’ACAMPORA,ARMANDO J.; BERNARDES,GERALDO J.S.; BELMONTE,LUIZ; MARTINS,THIAGO C.; BOBINSKI,FRANCIANE; CARGNIN-FERREIRA,EDUARDO; HOEPERS,ANDREZA; COMIM,CLARISSA M.; MARTINS,DANIEL F.; PIOVEZAN,ANNA P.
Abstract Silicone breast implant is associated with complications inherent to the surgical procedure. Prosthesis coating with polyurethane, however, commonly reduces the incidence of such complications. In this paper, the authors evaluated the inflammatory histomorphometric profile and oxidative damage associated to the implant of polyester urethane sheets. Forty-eight Wistar rats were divided into Sham or polyester urethane groups (n = 8/group) and underwent a polyester urethane implant in the dorsal skinfold. Tissue samples were collected on days seven, 30, and 90 after surgery and subjected to histomorphometric analysis and biochemical tests. Results were analyzed by one-way ANOVA (p ≤ 0.05). Peri-implant tissue samples exhibited characteristic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyurethane; Inflammation; Cytokines; Oxidative stress; Wistar rats.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502211

Ameliorative potential of Ocimum sanctum in chronic constriction injury-induced neuropathic pain in rats
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: KAUR,GURPREET; BALI,ANJANA; SINGH,NIRMAL; JAGGI,AMTESHWAR S.
The present study was designed to investigate the ameliorative potential of Ocimumsanctum and its saponin rich fraction in chronic constriction injury-induced neuropathic pain in rats. The chronic constriction injury was induced by placing four loose ligatures around the sciatic nerve, proximal to its trifurcation. The mechanical hyperalgesia, cold allodynia, paw heat hyperalgesia and cold tail hyperalgesia were assessed by performing the pinprick, acetone, hot plate and cold tail immersion tests, respectively. Biochemically, the tissue thio-barbituric acid reactive species, super-oxide anion content (markers of oxidative stress) and total calcium levels were measured. Chronic constriction injury was associated with the development of mechanical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic constriction injury; Ocimum sanctum; Saponin rich fraction; Calcium; Oxidative stress.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100417

Evaluation of antioxidant activity of grapevine leaves extracts (Vitis labrusca) in liver of Wistar rats
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: SCHAFFER,THAYS K.; WOHLENBERG,MARIANE F.; MEDEIROS,NIARA; MARTINS,JÉSSICA B.; AGOSTINI,FABIANA; FUNCHAL,CLÁUDIA; DANI,CAROLINE.
The aim of this study was to evaluate the hepatoprotection of organic and conventional grapevine leaves extract (Vitis labrusca). The total polyphenol content and the isolate polyphenols by HPLC were evaluate. The animals received intraperitoneal injections of saline or extracts (conventional or organic - 30 mg/kg) for 14 days. On day 15, the rats received carbon tetrachloride (CCl4) or mineral oil (i.p.). After 4h, the animals were euthanized. The analysis of the liver enzymes activity (AST, ALT, GGT) was performed using serum, obtained by blood and the levels of lipid peroxidation (TBARS), protein oxidation (carbonyl), and the activity of antioxidant enzymes superoxide dismutase and catalase were analyzed in the liver. The results showed that the organic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Carbon tetrachloride; Hepatoprotection; Grapes; Phenolic compounds.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100187

Attenuation of oxidative stress, inflammation and apoptosis by ethanolic and aqueous extracts of Crocus sativusL. stigma after chronic constriction injury of rats
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: AMIN,BAHAREH; ABNOUS,KHALIL; MOTAMEDSHARIATY,VAHIDEH; HOSSEINZADEH,HOSSEIN.
In our previous study, the ethanolic and aqueous extracts of Crocus sativus elicited antinociceptive effects in the chronic constriction injury (CCI) model of neuropathic pain. In this study, we explored anti-inflammatory, anti-oxidant and anti-apoptotic effects of such extracts in CCI animals. A total of 72 animals were divided as vehicle-treated CCI rats, sham group, CCI animals treated with the effective dose of aqueous and ethanolic extracts (200 mg/kg, i.p.). The lumbar spinal cord levels of proinflammatory cytokines including tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β) and interleukin 6 (IL-6), were evaluated at days 3 and 7 after CCI (n=3, for each group). The apoptotic protein changes were evaluated at days 3 and 7 by western blotting....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crocus sativus; Chronic constriction injury; Inflammatory cytokines; Oxidative stress; Apoptosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401821

Dextran Sulphate of Sodium-induced colitis in mice: antihyperalgesic effects of ethanolic extract of Citrus reticulata and potential damage to the central nervous system
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: HILEL,ALEXANDRE S.; GYSEMANS,BRUNA; LISBÔA,MARIA E.M.; HEYMANNS,ANA C.; FREIBERGER,VIVIANE; VENTURA,LETÍCIA; MAGNAGO,RACHEL F.; COMIM,CLARISSA M.; HOREWICS,VERÔNICA; MARTINS,DANIEL F.; BOBINSKI,FRANCIANE; PIOVEZAN,ANNA P.
Abstract Citrus species are widely related to antihyperalgesic and anti-inflammatory effects. The aim of this study was to investigate if treatment with ethanolic extract from peels of mature Citrus reticulata Blanco causes antihyperalgesic effects on the referred mechanical hyperalgesia in a model of dextran sulphate of sodium (DSS)-induced colitis in mice, as well as the possible oxidative damage in different regions of the brain induced by its inflammatory reaction. Antihyperalgesia (30 to 300 mg/kg) was investigated by behavioral response (frequency of response to von Frey filament stimulation) in Swiss mice, while damage to central nervous system was investigated through techniques that evaluated oxidative stress using male black C57 BL6 mice (n=8)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus reticulate; Medicinal plants; Inflammation; Oxidative stress; Central nervous system.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000603139

Red cabbage (Brassica oleracea L.) extract reverses lipid oxidative stress in rats
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: VEBER,BRUNO; CAMARGO,ANDERSON; DALMAGRO,ANA PAULA; BONDE,HENRIQUE LUIS P.; MAGRO,DÉBORA D. DAL; LIMA,DANIELA D. DE; ZENI,ANA LÚCIA B.
Abstract  Red cabbage (Brassica oleracea L. var. capitata f. rubra DC.) extract has been demonstrated hypolipidemic and antioxidant capacity. Herein, we investigated the effect of red cabbage aqueous extract (RC) or fenofibrate (FF) in oxidative stress induced by Triton WR-1339 in rats. The antioxidant capacity was evaluated through the superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities and, thiobarbituric reactive species (TBARS) and protein carbonyl (PC) levels in erythrocytes, liver, kidneys, cerebral cortex and hippocampus of male rats. The alterations promoted by Triton WR-1339 in enzymatic antioxidant defense in the liver, kidneys and hippocampus were reversed by RC or FF treatments. The TBARS and PC levels...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Red cabbage; Brassica oleracea; Oxidative stress; Antioxidants; Triton WR-1339.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100711

Protective Effect of Vitamin C on Triptolide-induced Acute Hepatotoxicity in Mice through mitigation of oxidative stress
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: XU,PENGJUAN; LI,YOUYOU; YU,ZHICHAO; YANG,LIN; SHANG,RONG; YAN,ZIHANG.
Abstract: Triptolide, a purified diterpenoid from the herb Tripterygium wilfordii Hook.f., was widely used to treat many diseases. However, the hepatotoxicity of triptolide limited its clinical use. Research showed oxidative stress played an important role in triptolide-induced liver injury. To investigate the effect of vitamin C, which was one of the most effective antioxidants, on triptolide-induced hepatotoxicity and its potential mechanism in mice. In the present study, acute liver injury was induced by intraperitoneal injection of triptolide and vitamin C was orally administered. The results showed treatment with vitamin C prevented the triptolide-induced liver injury by reducing the levels of aspartate transaminase from 286.86 to 192.48 U/mL and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitamin C; Triptolide; Liver; Oxidative stress; Mice.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300713

The effect of newly initiated exercise training on dynamic thiol / disulphide homeostasis in sedentary obese adults
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: CELIK,HAKIM; KILIC,TUGBA; KAPLAN,DAVUT S.; EREN,MEHMET A.; EREL,OZCAN; KARAKILCIK,ALI Z.; BAGCI,CAHIT.
Abstract: We studied dynamic thiol/disulphide homeostasis, an indicator of oxidative stress, to investigate the effects of newly initiated exercise training on sedentary obese adults. Seventeen sedentary obese adults and 15 normal-weight controls were included in the sample for this study. The obese adults were given a physical exercise training program that lasted twelve weeks. Before and after the exercise training program, blood samples were collected, and serum thiol/disulphide parameters were measured by using a novel technique. Before the start of the exercise training, it was observed that thiol/disulphide homeostasis was impaired, and this impairment was positively correlated with body mass index in sedentary obese adults because of the higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise; Obesity; Oxidative stress; Thiol / disulphide homeostasis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700805

Lipid peroxidation in female dogs bearing mammary gland carcinomas
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Souza,I.B.; Cardoso,C.V.; Poppe,S.C.; Bondan,E.F.
RESUMO O estresse oxidativo causa peroxidação lipídica e formação de substâncias reativas ao ácido tiobarbitúrico (TBARS), processo que está comprovadamente associado à progressão de neoplasias malignas em seres humanos. Por sua vez, espécies reativas de oxigênio (EROs) são produzidas no processo carcinogênico, de forma que a geração de EROs parece ser, ao mesmo tempo, causa e consequência dele. Em cães, a associação da peroxidação lipídica com a carcinogênese permanece ainda obscura, com estudos escassos, de resultados conflitantes, que, muitas vezes, incluem, dentro de um mesmo grupo amostral, animais portadores de tumores heterogêneos dos pontos de vista morfológico e comportamental, além de estes se apresentarem em estágios bastante distintos. Nesse...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Dogs; Cancer; Mammary gland; Oxidative stress; TBARS.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501335

(+)-Catechin and (-)-epigallocatechin gallate: are these promising antioxidant therapies for frozen goat semen?
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Silva,E.C.B.; Arruda,L.C.P.; Vieira,J.I.T.; Soares,P.C.; Guerra,M.M.P.
ABSTRACT The aim of this study was to evaluate the effects of different concentrations of (+)-catechin or (-)-epigallocatechin gallate (EGCG) on goat semen freezability. Poolsof semen were processed (Experiment 1: 0, 15, 25, 50, 75, or 100µM (+)-catechin; Experiment 2: 0, 15, 25, 50, 75, or 100µM EGCG) and frozen. After thawing, the samples were evaluated for kinematics, plasma membrane (PMi) and acrosome integrity, morphology, and oxidative stress, at 0 and 1h. In Experiment 1, at 0h, VSL and VAP were greater (P&lt;0.05) with 15µM than with 50 and 100; WOB was lower (P&lt;0.05) with 100µM than with 0, 15, and 25; and BCF was higher (P&lt;0.05) with 75 and 100µM than with 0. In turn, in Experiment 2, progressive motility was higher (P&lt;0.05) with0 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Cryopreservation; Flavonoids; Oxidative stress; Semen.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200521

High resveratrol or quercetin concentrations reduce the oscillation index of frozen goat semen
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Silva,E.C.B.; Arruda,L.C.P.; Silva,S.V.; Souza,H.M.; Guerra,M.M.P.
ABSTRACT The aim of this study was to evaluate the effect of different concentrations of trans-resveratrol or quercetin on the ability of goat sperm to withstand being frozen. Six pools of semen obtained from six male goats were processed with different concentrations of resveratrol or quercetin (Experiment 1: 0, 15, 25, 50, 75 or 100µM resveratrol; Experiment 2: 0, 15, 25, 50, 75 or 100µM quercetin) and frozen. After thawing, the semen was evaluated for sperm kinematics, plasma membrane and acrosome integrity, morphology and oxidative stress following 0 and 1h of incubation. Immediately after thawing (0h), wobble (oscillation index) in the groups treated with 100µM of quercetin or resveratrol was lower (P&lt;0.05) than in those treated with 0 and 25µM...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Flavonoid; No flavonoid; Oxidative stress; Semen freezing.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501237

Oxidative profiles of endurance horses racing different distances
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Siqueira,R.F.; Weigel,R.A.; Nunes,G. R.; Mori,C.S.; Fernandes,W.R.
Increased oxidative stress during prolonged endurance exercises may result in muscle damage, fatigue and decreased performance. An adequate stress response during training is critical to obtain improved results and high animal welfare standards. The aim of this study was to evaluate the red blood cell haemolysate concentrations of superoxide dismutase (SOD), glutathione peroxidase (GPx), reduced glutathione (GSH) and catalase (CAT) and the plasma concentrations of malondialdehyde (MDA) from endurance horses in different distances at high speed in a tropical climate. Fifteen horses were tested; five at 160km (18.54 - 17.16km/h race speed), five at 120km (21.53 - 17km/h race speed) and five at 80km (20.06 - 18.01km/h race speed). Blood samples were collected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horse; Oxidative stress; Endurance.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000200020

Reactive oxygen species in bovine embryo in vitro production
Provedor de dados: Biocell
Autores: Dalvit,G.C.; Cetica,P.D.; Pintos,L.N.; Beconi,M.T.
Oxidative modifications of cell components due to the action of reactive oxygen species (ROS) is one of the most potentially damaging processes for proper cell function. However, in the last few years it has been observed that ROS participate in physiological processes. The aim of this work was to determine ROS generation during in vitro production of bovine embryos. Cumulus-oocyte complexes were recovered by aspiration of antral follicles from ovaries obtained from slaughtered cows and cultured in medium 199 for 22 h at 39ºC in 5% CO2: 95% humidified air. In vitro fertilization was carried out in IVF-mSOF with frozenthawed semen in the same culture conditions and embryo in vitro culture in IVC-mSOF at 90% N2: 5% CO2: 5% O2. ROS was determined in denuded...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Reactive oxygen species; Oxidative stress; Oocyte; Embryo; Bovine.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200009

Biochemical-molecular markers in unilateral ureteral obstruction
Provedor de dados: Biocell
Autores: Manucha,Walter.
Congenital obstructive nephropathy is the primary cause of end-stagerenal disease in children. Rapid diagnosis and initiation of the treatment are vital to preserve function and/or to slow down renal injury. Obstr uctive uropathy effects-decline in the plasmatic renal flow and glomerular filtrationrate, interstitial infiltrate of leukocytes, significant decrease of the urine concentration, loss of the capacity to concentrate urine as well as fibrosis and apoptosisare a consequence of a variety of factors that work in complex ways and are still not fully understood. Mediators as angiotensin II, transforming growth factor-b (TGF-b) and nitric oxide (NO) have been implicated in congenital obstructive nephropathy. The renin-angiotensin system is regulated in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Unilateral ureteral obstruction; Interstitial fibrosis; Nitric oxide; Angiotensin II; Oxidative stress.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100001

Protective effect of bixin on carbon tetrachloride-induced hepatotoxicity in rats
Provedor de dados: Biological Research
Autores: Moreira,Priscila R; Maioli,Marcos A; Medeiros,Hyllana CD; Guelfi,Marieli; Pereira,Flávia TV; Mingatto,Fábio E.
BACKGROUND: The liver is an important organ for its ability to transform xenobiotics, making the liver tissue a prime target for toxic substances. The carotenoid bixin present in annatto is an antioxidant that can protect cells and tissues against the deleterious effects of free radicals. In this study, we evaluated the protective effect of bixin on liver damage induced by carbon tetrachloride (CCl4) in rats. RESULTS: The animals were divided into four groups with six rats in each group. CCl4 (0.125 mL kg-1 body wt.) was injected intraperitoneally, and bixin (5.0 mg kg-1 body wt.) was given by gavage 7 days before the CCl4 injection. Bixin prevented the liver damage caused by CCl4, as noted by the significant decrease in serum aminotransferases release....
Tipo: Journal article
Palavras-chave: Bixin; Carbon tetrachloride; Hepatotoxicity; Oxidative stress; Protective activity.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100072

Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E
Provedor de dados: Biological Research
Autores: Santana,Andréia T; Guelfi,Marieli; Medeiros,Hyllana C D; Tavares,Marco A; Bizerra,Paulo F V; Mingatto,Fábio E.
BACKGROUND: Gossypol is a chemical present in the seeds of cotton plants (Gossypium sp.) that reduces fertility in farm animals. Vitamin E is an antioxidant and may help to protect cells and tissues against the deleterious effects of free radicals. The aim of this study was to evaluate the mechanisms of reproductive toxicity of gossypol in rats and the protective effects of vitamin E. Forty Wistar rats were used, divided into four experimental groups (n = 10): DMSO/ saline + corn oil; DMSO/saline + vitamin E; gossypol + corn oil; and gossypol + vitamin E. RESULTS: Fertility was significantly reduced in male rats treated with gossypol in that a significant decrease in epididy-mal sperm count was observed (P &lt; 0.05) and the number of offspring was...
Tipo: Journal article
Palavras-chave: Gossypol; Fertility; Oxidative stress; Bioenergetics; Antioxidant system; Vitamin E.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100043

Co-operative effect of glutathione depletion and selenium induced oxidative stress on API and NFkB expression in testicular cells in vitro: insights to regulation of spermatogenesis
Provedor de dados: Biological Research
Autores: SHALINI,SONIA; BANSAL,MOHINDER P.
Previous studies have shown that transcription factors, API and NFkB exert important roles in the process by which selenium regulates spermatogenesis. Glutathione, an intracellular thiol, acts as a source of reducing power and aids in maintenance of the cellular redox status. The activities of selenium are closely related to the availability of glutathione. Presently, mouse testicular cells were cultured in the presence of BSO, a known glutathione depletor, to generate oxidative stress. Selenium (Se) was added as sodium selenite to these cells at concentrations of 0.5 µM and 1.5 µM. It was observed that at 1.5 µM, Se acted as a pro-oxidant and significantly decreased the redox ratio. RT PCR analysis revealed that cjun, cfos expression increased in...
Tipo: Journal article
Palavras-chave: API; BSO; GSH; NFkB; Oxidative stress; Selenium; Testicular cells.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400005

Endothelial cell oxidative stress and signal transduction
Provedor de dados: Biological Research
Autores: FONCEA,ROCIO; CARVAJAL,CRISTIAN; ALMARZA,CAROLINA; LEIGHTON,FEDERICO.
Endothelial dysfunction (ED) is an early event in atherosclerotic disease, preceding clinical manifestations and complications. Increased reactive oxygen species (ROS) have been implicated as important mechanisms that contribute to ED, and ROS’s may function as intracellular messengers that modulate signaling pathways. Several intracellular signal events stimulated by ROS have been defined, including the identification of two members of the mitogen activated protein kinase family (ERK1/2 and big MAP kinase, BMK1), tyrosine kinases (Src and Syk) and different isoenzymes of PKC as redox-sensitive kinases. ROS regulation of signal transduction components include the modification in the activity of transcriptional factors such as NFkB and others that result in...
Tipo: Journal article
Palavras-chave: Oxidative stress; Signal transduction; Gene expression; Endothelial cell; Atherosclerosis; Antioxidants.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200008

Protective role of vitamins C and E in diclorvos-induced oxidative stress in human erythrocytes in vitro
Provedor de dados: Biological Research
Autores: Eroglu,Sema; Pandir,Dilek; Uzun,Fatma G; Bas,Hatice.
Organophosphate (OP) pesticides such as dichlorvos (DDVP) intoxication has been shown to produce oxidative stress due to the generation of free radicals, which alter the antioxidant defense system in erythrocytes. In this study, the effects of DDVP (1, 10, 100 µM) or DDVP + vitamin C (VC; 10 µM) or vitamin E (VE; 30 µM), on the levels of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities in human erythrocytes were examined in vitro. There were no statistical differences between all groups for 1 µM concentration of DDVP. Treatment with DDVP alone produced an increase in the level of MDA and decreased activities of antioxidant enzymes (P &lt; 0.05). Groups treated with vitamins and DDVP showed...
Tipo: Journal article
Palavras-chave: Antioxidant enzyme; Dichlorvos; Oxidative stress; Vitamin; Malondialdehyde.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100005

Iron and glutathione at the crossroad of redox metabolism in neurons
Provedor de dados: Biological Research
Autores: ARACENA,PAULA; AGUIRRE,PABLA; MUÑOZ,PABLO; NÚÑEZ,MARCO T.
Neurons, as non-dividing cells, encounter a myriad of stressful conditions throughout their lifespan. In particular, there is increasing evidence that iron progressively accumulates in the brain with age and that iron-induced oxidative stress is the cause of several forms of neurodegeneration. Here, we review recent evidence that gives support to the following notions: 1) neuronal iron accumulation leads to oxidative stress and cell death; 2) neuronal survival to iron accumulation associates with decreased expression of the iron import transporter DMT1 and increased expression of the efflux transporter IREG1; and 3) the adaptive process of neurons towards iron-induced oxidative stress includes a marked increase in both the expression of the catalytic...
Tipo: Journal article
Palavras-chave: Neurons; Iron metabolism; Iron accumulation; Oxidative stress; Glutathione metabolism; Neurodegeneration.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100017

Cobalt oxide nanoparticles aggravate DNA damage and cell death in eggplant via mitochondrial swelling and NO signaling pathway
Provedor de dados: Biological Research
Autores: Faisal,Mohammad; Saquib,Quaiser; Alatar,Abdulrahman A.; Al-Khedhairy,Abdulaziz A.; Ahmed,Mukhtar; Ansari,Sabiha M.; Alwathnani,Hend A.; Dwivedi,Sourabh; Musarrat,Javed; Praveen,Shelly.
BACKGROUND: Despite manifold benefits of nanoparticles (NPs), less information on the risks of NPs to human health and environment has been studied. Cobalt oxide nanoparticles (Co3O4-NPs) have been reported to cause toxicity in several organisms. In this study, we have investigated the role of Co3O4-NPs in inducing phytotoxicity, cellular DNA damage and apoptosis in eggplant (Solanum melongena L. cv. Violetta lunga 2). To the best of our knowledge, this is the first report on Co3O4-NPs showing phytotoxicity in eggplant. RESULTS: The data revealed that eggplant seeds treated with Co3O4-NPs for 2 h at a concentration of 1.0 mg/ml retarded root length by 81.5 % upon 7 days incubation in a moist chamber. Ultrastructural analysis by transmission electron...
Tipo: Journal article
Palavras-chave: Cobalt oxide nanoparticles; Nanotoxicity; DNA damage; Apoptosis; Oxidative stress.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100020

Cytoprotective and suicidal signaling in oxidative stress
Provedor de dados: Biological Research
Autores: Videla,Luis A.
Oxidative stress is an imbalance between pro-oxidants and antioxidants in favor of the pro-oxidants, leading to different responses depending on the level of pro-oxidants achieved and the duration of exposure. In this article, we discuss the cytoprotective or suicidal signaling mechanisms associated with oxidative stress by addressing: (i) the development of an acute and mild pro-oxidant state by thyroid hormone administration eliciting the redox upregulation of the expression of proteins affording cell protection as a preconditioning strategy against ischemia-reperfusion liver injury; and (ii) the role of prolonged and severe oxidative stress and insulin resistance as determinant factors in the pathogenesis of non-alcoholic fatty liver disease associated...
Tipo: Journal article
Palavras-chave: Oxidative stress; Thyroid hormone; Liver preconditioning; Obesity; Insulin resistance; Non-alcoholic fatty liver disease.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300014

Effect of iron on the activation of the MAPK/ERK pathway in PC12 neuroblastoma cells
Provedor de dados: Biological Research
Autores: MUÑOZ,PABLO; ZAVALA,GABRIELA; CASTILLO,KAREN; AGUIRRE,PABLA; HIDALGO,CECILIA; NÚÑEZ,MARCO T.
Recent evidence suggests that reactive oxygen species function as second messenger molecules in normal physiological processes. For example, activation of N-Methyl-D-Aspartate receptor results in the production of ROS, which appears to be critical for synaptic plasticity, one of the cellular mechanisms that underlie learning and memory. In this work, we studied the effect of iron in the activation of MAPK/ERK pathway and on Ca2+ signaling in neuronal PC12 cells. We found that iron-dependent generation of hydroxyl radicals is likely to modulate Ca2+ signaling through RyR calcium channel activation, which, in turn, activates the MAPK/ERK pathway. These findings underline the relevance of iron in normal neuronal function.
Tipo: Journal article
Palavras-chave: NMDA receptor; Calcium; Iron; Oxidative stress; MAPK/ERK; Hippocampal slices; Long-term potentiation.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100021

Effects of weight loss on liver and erythrocyte polyunsaturated fatty acid pattern and oxidative stress status in obese patients with non-alcoholic fatty liver disease
Provedor de dados: Biological Research
Autores: ELIZONDO,ALEJANDRA; ARAYA,JULIA; RODRIGO,RAMÓN; SIGNORINI,CINZIA; SGHERRI,CRISTIANA; COMPORTI,MARIO; PONIACHIK,JAIME; VIDELA,LUIS A.
Our aim was to study the influence of weight loss on the fatty acid (FA) composition of liver and erythrocyte phospholipids and oxidative stress status in obese, non-alcoholic, fatty liver disease (NAFLD) patients. Seven obese NAFLD patients who underwent subtotal gastrectomy with a gastro-jejunal anastomosis in roux and Y were studied immediately and 3 months after surgery. Seven non-obese patients who underwent anti-reflux surgery constituted the control group. Serum F2-isoprostane levels were measured by GS/NICI-MS/MS and FA composition was determined by GC. At the time of surgery, controls and obese patients exhibited a hepatic polyunsaturated fatty acid (PUFA) pattern that correlated with that of erythrocytes. Three months after surgery, NAFLD...
Tipo: Journal article
Palavras-chave: Polyunsaturated fatty acids; Oxidative stress; Obesity; Weight loss.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100008

Effectiveness of zinc in modulating perinatal effects of arsenic on the teratological effects in mice offspring
Provedor de dados: Biological Research
Autores: Ahmad,Mohammad; Wadaa,Mohammad A. M; Farooq,Muhammad; Daghestani,Maha H; Sami,Ahmed S.
Exposure to arsenic via drinking water is considered as a worldwide problem. Studies have shown that arsenic exposure during pregnancy affects embryogenesis and offspring development in rats and mice. Zinc as a micronutrient regulates many physiological functions, including an antioxidative role under various toxic conditions. However, studies on the perinatal protective effect of zinc on offspring need further attention. The present study was designed to evaluate the potential protective role of zinc in mitigating the adverse effects in the offspring of arsenic exposure during pregnancy. The arsenic (40mg/kg body weight) and zinc (4% w/v) doses formed the only drinking fluid source for the experimental groups of dams during the perinatal period of the...
Tipo: Journal article
Palavras-chave: Arsenic; Zinc; Perinatal; Mice offspring; Sensory motor reflexes; Behavior; Oxidative stress.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200003

Relationship between severity of adult community-acquired pneumonia and impairment of the antioxidant defense system
Provedor de dados: Biological Research
Autores: Castillo,Rodrigo L; Carrasco,Rodrigo A; Álvarez,Pedro I; Ruiz,Mauricio; Luchsinger,Vivian; Zunino,Enna; Martínez,María A; Avendaño,Luis F.
Oxidant/antioxidant imbalance has been reported in some infectious diseases, including community-acquired pneumonia (CAP). The aim was to assess the antioxidant status in adults with CAP and its relationship with clinical severity at admission. Fifty-nine patients with CAP were enrolled and categorized at admission by the FINE score, from July 2010 to October 2012. In the same period 61 controls were enrolled. Plasma samples were obtained at admission for determination of the ferric reducing ability of plasma (FRAP) and lipid peroxidation (8-isoprostane). Erythrocyte reduced (GSH)/oxidized (GSSG) glutathione, malondialdehyde (MDA) and antioxidant enzyme activity were assessed. Antioxidant status in adults with CAP represented by FRAP and the GSH/GSSG ratio...
Tipo: Journal article
Palavras-chave: Pneumonia; Oxidative stress; Reduced glutathione; Lipid peroxidation.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200013

Lipid peroxidation and antioxidants in hyperlipidemia and hypertension
Provedor de dados: Biological Research
Autores: MORIEL,PATRICIA; PLAVNIK,FRIDA L; ZANELLA,MARIA T; BERTOLAMI,MARCELO C; ABDALLA,DULCINEIA SP.
Lipid peroxidation and lipid-derived oxidized products have been implicated in the pathogenesis of a variety of human diseases. To clarify the role of oxidative stress in essential hypertension and hypercholesterolemia the in vitro oxidative susceptibility of LDL, the antioxidant status and the lipid peroxide content of blood plasma were examined in hypercholesterolemic (HC), hypertensive (H), hypercholesterolemic/hypertensive (HH) and normolipidemic/normotensive subjects (N). Plasma ascorbate and lipid-soluble antioxidants were lower, while LDL oxidizability, CE-OOH and TL-OOH were higher in H, HC, and HH groups than in the N group. No difference was observed among groups for PL-OOH and isoprostanes. In summary, the results show that: 1) lipid- and...
Tipo: Journal article
Palavras-chave: Lipoprotein oxidation; Lipid peroxides; Isoprostanes; Antioxidants; Atherosclerosis; Oxidative stress.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200010

Impact of the Training Program on Lipid Profile and Cardiac Health
Provedor de dados: Biological Research
Autores: BURNEIKO,REGINA COELI; DINIZ,YEDA SANT'ANA; FAINE,LUCIANE APARECIDA; MACHADO GALHARDI,CRISTIANO; PADOVANI,CARLOS ROBERTO; NOVELLI,ETHEL LOURENZI B.; CICOGNA,ANTONIO CARLOS.
The aim of this study was to investigate the effects of training programs on serum lipid profile and myocardial oxidative stress. Male Wistar rats (2 mo-old) were divided into three groups (n=8): sedentary (S), loadless trained (T) and trained-overload 2% body weight (TL). T and TL were trained through swimming for 9 weeks. T and TL rats had increased myocardial lipoperoxide (TBA) and lipid hydroperoxide (HP), whereas HP was higher in TL than in T animals. Superoxide dismutase (SOD) activities were lowest in TL. Myocardial glutathione peroxidase (GSH-Px) was lower in TL than in T and S rats. TL decreased HDL-cholesterol and increased LDL-cholesterol. The serum lactate dehydrogenase and TBA were increased, while SOD and GSH-Px activities were decreased in...
Tipo: Journal article
Palavras-chave: Training; Lipid profile; Oxidative stress; Heart; Serum.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100006

Vitamin C and oxidative stress in the seminiferous epithelium
Provedor de dados: Biological Research
Autores: Angulo,Constanza; Maldonado,Rodrigo; Pulgar,Eduardo; Mancilla,Héctor; Córdova,Alex; Villarroel,Franz; Castro,Maite A; Concha,Ilona I.
In this article, we focus on the fundamental role of vitamin C transporters for the normal delivery of vitamin C to germ cells in the adluminal compartment of seminiferous tubules. We argue that the redox status within spermatozoa or in semen is partly responsible for the etiology of infertility. In this context, antioxidant defence plays a critical role in male fertility. Vitamin C, a micronutrient required for a wide variety of metabolic functions, has long been associated with male reproduction. Two systems for vitamin C transport have been described in mammals. Facilitative hexose transporters (GLUTs), with 14 known isoforms to date, GLUT1-GLUT14, transport the oxidized form of vitamin C (dehydroascorbic acid) into the cells. Sodium ascorbic acid...
Tipo: Journal article
Palavras-chave: Ascorbic acid; Oxidative stress; GLUT; Sertoli cells; SVCT; Vitamin C transporters.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200009

Iron homeostasis in the lung
Provedor de dados: Biological Research
Autores: GHIO,ANDREW J; TURI,JENNIFER L; YANG,FUNMEI; GARRICK,LAURA M; GARRICK,MICHAEL D.
Iron is essential for many aspects of cellular function. However, it also can generate oxygen-based free radicals that result in injury to biological molecules. For this reason, iron acquisition and distribution are tightly regulated. Constant exposure to the atmosphere results in significant exposure of the lungs to catalytically active iron. The lungs have a mechanism for detoxification to prevent associated generation of oxidative stress. Those same proteins that participate in iron uptake in the gut are also employed in the lung to transport iron intracellularly and sequester it in an inactive form within ferritin. The release of metal is expedited (as transferrin and ferritin) from lung tissue to the respiratory lining fluid for clearance by the...
Tipo: Journal article
Palavras-chave: Lung diseases; Oxidative stress; SLC11A2 protein; SLC40A1 protein.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100008

Effects of isoflavone on oxidative stress parameters and homocysteine in postmenopausal women complaining of insomnia
Provedor de dados: Biological Research
Autores: BRANDÃO,LETÍCIA C; HACHUL,HELENA; BITTENCOURT,LIA R; BARACAT,EDMUND C; TUFIK,SERGIO; D'ALMEIDA,VÂNIA.
Sleep disorders have an increased incidence after menopause. The objective of this work was to evaluate the effects of isoflavone on some oxidative stress markers in postmenopausal women complaining of insomnia. Women aged between 50-65 years (n=38) were recruited and assigned to a double-blind placebo controlled study for 4 months. The treated group received 100 mg/day of isoflavones. Blood collections were conducted on three different occasions to assess total glutathione; superoxide dismutase and catalase in erythrocytes; lipid peroxidation; and homocysteine plasma concentrations. No differences between the groups were observed. However, all the patients seem to improve their oxidative stress status and homocysteine concentration after treatment....
Tipo: Journal article
Palavras-chave: Homocysteine; Isoflavones; Insomnia; Menopause; Oxidative stress.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300002

Selected spices and their combination modulate hypercholesterolemia-induced oxidative stress in experimental rats
Provedor de dados: Biological Research
Autores: Otunola,Gloria A; Oloyede,Oyelola B; Oladiji,Adenike T; Afolayan,Anthony J.
BACKGROUND: Effect of aqueous extracts of Allium sativum (garlic), Zingiber officinale (ginger), Capsicum fructensces (cayenne pepper) and their mixture on oxidative stress in rats fed high Cholesterol/high fat diet was investigated. Rats were randomly distributed into six groups (n&amp;#8201;=&amp;#8201;6) and given different dietary/spice treatments. Group 1 standard rat chow (control), group 2, hypercholesterolemic diet plus water, and groups 3, 4, 5, 6, hypercholesterolemic diet with 0.5 ml 200 mg&amp;#8201;·&amp;#8201;kg-1 aqueous extracts of garlic, ginger, cayenne pepper or their mixture respectively daily for 4 weeks. RESULTS: Pronounced oxidative stress in the hypercholesterolemic rats evidenced by significant (p&amp;#8201;&lt;&amp;#8201;0.05)...
Tipo: Journal article
Palavras-chave: Lipid peroxidation; Oxidative stress; Spices; Hypercholesterolemia; ROS.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100005

Hepatoprotective effect of Cinnamon extracts against carbon tetrachloride induced oxidative stress and liver injury in rats
Provedor de dados: Biological Research
Autores: MOSELHY,SAID S; ALI,HUSEIN K. H.
Cinnamon is used to flavor most foods in Arabian countries. The aim of this study was to evaluate the medicinal importance, reflecting an important trend in research. The hepatoprotective activity of aqueous and ethanolic extracts of cinnamon was investigated against carbon tetrachloride (CC1(4)) induced lipid peroxidation and hepatic injury in rats. The elevated serum AST and ALT enzymatic activities induced by CC1(4) were significantly restored to near normal by oral administration of 200 mg/kg of either extracts once daily for 7 days, as compared to untreated rats. There was a significant elevation in the level of liver malondialdhyde (MDA), while the activities of antioxidant enzymes superoxide dismutase and catalase (SOD and CAT) were significantly...
Tipo: Journal article
Palavras-chave: Cinnamon extracts; CCL; Oxidative stress; Rats.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100009

Melatonin Protects the Heart, Lungs and Kidneys from Oxidative Stress under Intermittent Hypobaric Hypoxia in Rats
Provedor de dados: Biological Research
Autores: Farías,Jorge G; Zepeda,Andrea B; Calaf,Gloria M.
Melatonin (N-acetyl-5-methoxytryptamine) is the main secretory product of the pineal gland in all mammals including humans, but it is also produced in other organs. It has been previously demonstrated to be a powerful organ-protective substance under oxidative stress conditions. The aim of this study was to evaluate the protective effect of melatonin in several organs such as heart, lung, kidney, and of the reproductive system, such as testis and epididymis in animals exposed to intermittent hypobaric hypoxia and therefore exposed to oxidative stress and analyzed by lipid peroxidation. Ten-week-old male Wistar rats were divided into 6 groups for 96 hours during 32 days under: 1) Normobaric conditions, 2) plus physiologic solution, 3) plus melatonin, 4)...
Tipo: Journal article
Palavras-chave: Intermittent hypobaric hypoxia; Lipid peroxidation; Melatonin; Oxidative stress.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100011

Wine grape pomace flour improves blood pressure, fasting glucose and protein damage in humans: a randomized controlled trial
Provedor de dados: Biological Research
Autores: Urquiaga,Inés; D'Acuna,Sonia; Pérez,Druso; Dicenta,Sara; Echeverria,Guadalupe; Rigotti,Attilio; Leighton,Federico.
BACKGROUND: The Mediterranean diet is a healthy diet with positive scientific evidence of preventing chronic diseases. Bioactive components support the healthy properties of the Mediterranean diet. Antioxidants and fiber, two components of the Mediterranean diet, are key functional nutrients for healthy eating and nutrition. Wine grape pomace is a rich source of these dietary constituents and may be beneficial for human health. Our hypothesis was that the intake of red wine grape pomace flour (WGPF) prepared from red wine grapes (Cabernet Sauvignon variety) reduced the metabolic syndrome in humans. To evaluate the effect of WGPF on components of metabolic syndrome we design a 16-week longitudinal intervention study. Thirty-eight males, 30-65 years of age,...
Tipo: Journal article
Palavras-chave: Antioxidant; Dietary intervention; Fiber; Metabolic syndrome; Oxidative stress; Wine grape pomace.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100049

Plant Polyphenol Antioxidants and Oxidative Stress
Provedor de dados: Biological Research
Autores: URQUIAGA,INES; LEIGHTON,FEDERICO.
In recent years there has been a remarkable increment in scientific articles dealing with oxidative stress. Several reasons justify this trend: knowledge about reactive oxygen and nitrogen species metabolism; definition of markers for oxidative damage; evidence linking chronic diseases and oxidative stress; identification of flavonoids and other dietary polyphenol antioxidants present in plant foods as bioactive molecules; and data supporting the idea that health benefits associated with fruits, vegetables and red wine in the diet are probably linked to the polyphenol antioxidants they contain.In this review we examine some of the evidence linking chronic diseases and oxidative stress, the distribution and basic structure of plant polyphenol antioxidants,...
Tipo: Journal article
Palavras-chave: Oxidative stress; Antioxidant; Plant polyphenol; Flavonoid; Chronic diseases; Diet.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200004

Compensatory responses induced by oxidative stress in Alzheimer disease
Provedor de dados: Biological Research
Autores: MOREIRA,PAULA I; ZHU,XIONGWEI; LIU,QUAN; HONDA,KAZUHIRO; SIEDLAK,SANDRA L; HARRIS,PEGGY L; SMITH,MARK A.; PERRY,GEORGE.
Oxidative stress occurs early in the progression of Alzheimer disease, significantly before the development of the pathologic hallmarks, neurofibrillary tangles and senile plaques. In the first stage of development of the disease, amyloid-&amp;#946; deposition and hyperphosphorylated tau function as compensatory responses and downstream adaptations to ensure that neuronal cells do not succumb to oxidative damage. These findings suggest that Alzheimer disease is associated with a novel balance in oxidant homeostasis.
Tipo: Journal article
Palavras-chave: Alzheimer disease; Antioxidant therapy; Mitochondria; Oxidative stress; Redox metals.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100002

Protective effects of lycopene on oxidative stress, proliferation and autophagy in iron supplementation rats
Provedor de dados: Biological Research
Autores: Liu,Chongbin; Wang,Rui; Zhang,Bo; Hu,Chen; Zhang,Hong.
Lycopene is common in diet and known for its antioxidant activities. However, the impact of lycopene on iron metabolism is poorly investigated. In this study, we hypothesize that lycopene can prevent iron-mediated oxidative stress, proliferation and autophagy in liver and use a rat model of nutritional iron supplementation to confirm its intervention in these defence mechanisms. We found that iron supplementation induced cell proliferation predominantly in non parenchymal cells compared with hepatocytes, but not apoptosis. In addition, iron was accumulated within the hepatic lysosomes where it triggered autophagy as evidenced by the formation of autophagic vesicles detected by LC3-B staining. Iron supplementation also induced morphologic alterations of the...
Tipo: Journal article
Palavras-chave: Autophagy; Iron supplementation; Lycopene; Oxidative stress; Proliferation.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200011

Antioxidant and anti hyperglycemic role of wine grape powder in rats fed with a high fructose diet
Provedor de dados: Biological Research
Autores: Hernández-Salinas,Romina; Decap,Valerie; Leguina,Alberto; Cáceres,Patricio; Perez,Druso; Urquiaga,Ines; Iturriaga,Rodrigo; Velarde,Victoria.
BACKGROUND: Metabolic syndrome is a growing worldwide health problem. We evaluated the effects of wine grape powder (WGP), rich in antioxidants and fiber, in a rat model of metabolic syndrome induced by a high fructose diet. We tested whether WGP supplementation may prevent glucose intolerance and decrease oxidative stress in rats fed with a high fructose diet. METHODS: Male Sprague-Dawley rats weighing 180 g were divided into four groups according to their feeding protocols. Rats were fed with control diet (C), control plus 20 % WGP (C + WGP), 50 % high fructose (HF) or 50 % fructose plus 20 % WGP (HF + WGP) for 16 weeks. Blood glucose, insulin and triglycerides, weight, and arterial blood pressure were measured. Homeostasis model assessment (HOMA) index...
Tipo: Journal article
Palavras-chave: Insulin resistance; Oxidative stress; Metabolic syndrome; Wine grape powder.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100053

The role of thyroid hormone calorigenesis in the redox regulation of gene expression
Provedor de dados: Biological Research
Autores: VARELA,PATRICIA; TAPIA,GLADYS; FERNÁNDEZ,VIRGINIA; VIDELA,LUIS A.
Thyroid hormone (TH; 3,3',5-triiodothyronine, T3) is required for the normal function of most tissues, with major effects on 0(2) consumption and metabolic rate. These are due to transcriptional activation of respiratory genes through the interaction of T3-liganded TH receptors with TH response elements or the activation of intermediate factors, with the consequent higher production of reactive 0(2) species (ROS) and antioxidant depletion. T3-induced oxidative stress in the liver triggers the redox upregulation of the expression of cytokines (tumor necrosis factor-á [TNF-á], interleukin-10), enzymes (inducible nitric oxide synthase, manganese superoxide dismutase), and anti-apoptotic proteins (Bcl-2), via a cascade initiated by TNF-á produced by Kupffer...
Tipo: Journal article
Palavras-chave: Thyroid hormone; Calorigenesis; Oxidative stress; Gene expression.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500004

Upregulation of liver inducible nitric oxide synthase following thyroid hormone preconditioning: suppression by N-acetylcysteine
Provedor de dados: Biological Research
Autores: FERNÁNDEZ,VIRGINIA; TAPIA,GLADYS; VARELA,PATRICIA; CORNEJO,PAMELA; VIDELA,LUIS A.
3,3-5-L-Triiodothyronine (T3) exerts significant protection against ischemia-reperfusion (IR) liver injury in rats. Considering that the underlying mechanisms are unknown, the aim of this study was to assess the involvement of inducible nitric oxide synthase (iNOS) expression and oxidative stress in T3 preconditioning (PC). Male Sprague-Dawley rats given a single dose of 0.1 mg of T3/kg were subjected to 1-hour ischemia followed by 20 hours reperfusion, in groups of animals pretreated with 0.5 g of N-acetylcysteine (NAC)/kg 0.5-hour prior to T3 or with the respective control vehicles. At the end of the reperfusion period, liver samples were taken for analysis of iNOS mRNA levels (RT-PCR), liver NOS activity, and hepatic histology. T3 protected against...
Tipo: Journal article
Palavras-chave: Thyroid hormone; Oxidative stress; Inducible nitric oxide synthase; Liver preconditioning; Ischemia-reperfusion injury; N-acetylcysteine.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400010

Potential of hydrogen (pH) differentially modulates cadmium stress response in abscisic acid-deficient sitiens tomato mutant
Provedor de dados: Bragantia
Autores: Pompeu,Georgia Bertoni; Ambrosano,Guilherme Bovi; Vilhena,Milca Bartz; Carvalho,Rogério Falleiros; Gratão,Priscila Lupino; Andrino,Felipe; Lira,Simone Possedente de; Azevedo,Ricardo Antunes.
ABSTRACT Uptake of nutrients and cadmium (Cd) are dependent upon many factors, including plant species, ions concentration and pH. Tolerance to Cd-induced oxidative stress can be modulated by phytohormones such as abscisic acid (ABA), which induce the production of reactive oxygen species, activating proteins and enzymes involved in stress response and possibly stress tolerance. The present study aimed to evaluate the biochemical variations induced by Cd in ABA-deficient sitiens tomato mutant (sit) and its wild-type counterpart, Micro-Tom (MT), grown at different pH conditions. The plants were cultivated in nutrient solution (pH 5, 6 and 7; 20-days) and were then further grown over a 48-h period in 0 or 50 ?M CdCl2 at pH 6. Before Cd addition, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant enzymes; Micro-Tom; Mineral nutrition; Oxidative stress; Solanum lycopersicum.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052019000300317

Genotypic differences in cyanogenic glycosides levels of compatible Prunus persica P. persica and incompatible P. persica P. mume combinations
Provedor de dados: Bragantia
Autores: Pereira,Ivan dos Santos; Pina,Ana; Antunes,Luis Eduardo Correa; Campos,Ângela Diniz; Fachinello,José Carlos.
ABSTRACT Graft incompatibility is a phenomenon associated with complex physiological, biochemical, and genetic interactions between scion and rootstock. The main objective of this work was to assess the role of cyanogenic glycosides (CGs), amygdalin and prunasin, in the graft incompatibility of Prunus and possible biochemical effects in compounds of the phenylpropanoid pathway. Graft compatibility, amygdalin and prunasin content, phenylalanine ammonia-lyase activity, total phenolic compounds content and antioxidant activity, were studied in different graft combinations (Chimarrita/Capdeboscq; Chimarrita/Tsukuba 1; Chimarrita/Umezeiro; Maciel/Capdeboscq; Maciel/’Tsukuba 1; Maciel/Umezeiro) and ungrafted genotypes. The results indicate that there was graft...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Graft incompatibility; Amygdalin; Prunasin; Phenylalanine ammonia-lyase; Oxidative stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052018000100001

Aluminum stress tolerance in potato genotypes grown with silicon
Provedor de dados: Bragantia
Autores: Dorneles,Athos Odin Severo; Pereira,Aline Soares; Sasso,Victória Martini; Possebom,Gessieli; Tarouco,Camila Peligrinotti; Schorr,Márcio Renan Weber; Rossato,Liana; Ferreira,Paulo Ademar Avela; Tabaldi,Luciane Almeri.
ABSTRACT Potato cultivation is widespread around the world, being exposed to several abiotic stresses, including soils with high aluminum (Al) availability. Silicon (Si) is recognized for alleviating the stress caused by Al in various plant species. Thus, the aim of this study was to investigate the potential of Si to mitigate the oxidative stress caused by Al in potato genotypes, exhibiting differential sensitivity toward this element. Plants of the Al-sensitive genotype (SMIJ319-7) and Al-tolerant genotype (SMIF212-3) were grown for two weeks in a hydroponic system with the nutrient solution containing combinations of Al (0 and 1.85 mM) and Si (0, 0.5 and 1.0 mM). At the end of the experiment, photosynthetic parameters, pigment content, root and shoot...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum tuberosum; Beneficial elements; Enzyme activity; Oxidative stress; Toxic elements.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052019000100012

Physiological and ultrastructural analysis reveal the absence of a defined abscission zone in coffee fruits
Provedor de dados: Bragantia
Autores: Brandão,Isabel Rodrigues; Silva,Dayane Meireles da; Souza,Kamila Rezende Dázio de; Boas,Lissa Vasconcellos Vilas; Santos,Meline de Oliveira; Silva,Fábio Moreira da; Alves,Jose Donizeti.
ABSTRACT In order to investigate the existence of a possible abscission zone in the binding region between the peduncle and the coffee fruit, as well as the biochemical events involved in fruit ripening, we studied peduncles and fruits of Coffea arabica L. cv. “Icatu amarelo”. Coffee fruits were harvested at different maturation stages by using specific intervals of detachment force. It was observed a breakdown in the cells of the connecting region between peduncles and fruits throughout the maturation process. This disruption is probably caused by increased activity of the cell wall-loosening enzymes (polygalacturonase and cellulase) as well as by higher levels of lipid peroxidation in the final maturation stages. In general, physiological differences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea arabica; Icatu amarelo; Mechanized harvesting; Degrading enzyme; Oxidative stress.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052016000400386

The Dynamic of some Biochemical Indicators at Species of Medicinal Plants from Areas of Suceava County, Romania, Emerged under the Mining Pollution Influence
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Todirascu-Ciornea,Elena; Dumitru,Gabriela.
ABSTRACT It is known the fact that the mining causes huge damages on the environment having negative biologic effects extremely severe on some groups of organisms of the food chain. The pollutants causes major damages at the level of leaves' cuticles, the vegetation reacting through irreversible modifications at the tissue's level and at substances and energy metabolism, like a riposte to the apparition of the stress induced by the ROS, stress that creates real disturbances on physiological and biochemical processes. Considering the fact that, on the one hand, the activity of oxidoreductases is strongly influenced by the presence of the pollutants factors, with direct effect on the oxidative's stress production, and on the other hand that the plants react...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Mint; Plantain; Pollution.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100344

Oxidative stress during rehabilitation from protein malnutrition associated with aerobic exercise in rats
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Prada,Francisco José Andriotti; Macedo,Denise Vaz; Mello,Maria Alice Rostom de.
This study was designed to evaluate biomarkers of oxidative stress in rats with or without aerobic exercise during recovery from protein malnutrition. From the 30th to the 90th day of life, male Wistar rats were fed a low protein diet (LP + 6%) followed by a normal protein diet (NP = 17%) until the 120th day and separated in two groups: sedentary (S) and exercise trained (E = swimming 1h/day, 5 days/week, with from the 90th to the 120th day). Rats fed a normal protein diet were used as controls. Results showed that physical exercise had beneficial effects on body weight gain during nutrition rehabilitation. Erythrocytes catalase and glutathione reductase (biomarkers of the antioxidant system) were significantly reduced in all groups in comparison to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein malnutrition; Nutritional recovery; Exercise; Oxidative stress.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100006

Protective Effects of Edaravone against Methamphetamine-Induced cardiotoxicity
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Koohsari,Motahareh; Shaki,Fatemeh; Jahani,Daniel.
ABSTRACT Methamphetamine (METH) is widely abused in worldwide. METH use could damage the dopaminergic system and induce cardiotoxicity via oxidative stress and mitochondrial dysfunction. Edaravone, a sedative-hypnotic agent, is known for it's antioxidant properties. In this study we used edaravone for attenuating of METH-induced cardiotoxicity in rats. The groups (six rats in each group) were as follows: control, METH (5 mg/kg IP) and edaravone (5, 10 and 20 mg/kg, IP) was administered 30 min before METH. After 24 hours, animals were killed, heart tissue was separated and mitochondrial fraction was isolated and oxidative stress markers were measured. Edaravone significantly (p&lt;0.05) protected the heart against lipid peroxidation by inhibition of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Edaravone; Methamphetamine; Cardiotoxicity; Oxidative stress; Mitochondria.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100342

The Evolving Role of Erythropoietin in Lung of Irradiated Rats
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Ashry,Omaima Mohamed; Hasan,Hesham Farouk.
ABSTRACT The aim of the present study is to evaluate the possible curative effect of erythropoietin (EPO) on radiation-induced damage to the lung and its renin angiotensin system. EPO (200 U/100g) was i.p. injected to male rats one hour post 6 Gy whole body gamma irradiation. The animals were sacrificed after 14 days post irradiation. Irradiation induced significant drop of haematological values, bone marrow (BM) count, lung oxidative stress markers, glutathione (GSH) and superoxide dismutase(SOD) associated with significant elevation of malondialdehyde (MDA), advanced oxidation protein product (AOPP) and nitric oxide (NO) besides serum inflammatory markers, tumor necrosis factor alpha (TNF-α) and lactate dehydrogenase (LDH). Also serum and lung renin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Erythropoietin; Radiation; Inflammation; Oxidative stress.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100312

Lead Nitrate Induced Testicular Toxicity in Diabetic and Non-Diabetic Rats: Protective Role of Sodium Selenite
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Apaydin,Fatma Gokce; Kalender,Suna; Bas,Hatice; Demir,Filiz; Kalender,Yusuf.
Among heavy met als, lead is one of the common pollutants found in the environment and biological system. In the present study, streptozotocin-induced diabetic and normal non-diabetic male Wistar rats were given sodium selenite (1.0 mg/kg bw), lead nitrate (22.5 mg/kg bw) and sodium selenite plus lead nitrate (1.0 mg/kg+22.5 mg/kg bw, respectively) through gavage. At the end of 4th week, malondialdehyde (MDA) levels, antioxidant enzyme activities [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST)], and histopathological changes of testes were investigated compared to the control group. No significant differences were observed between the control and sodium selenite treated groups. However, lead...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Testicular toxicity; Lead nitrate; Sodium selenite; Streptozotocin; Oxidative stress; Histopathology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100068

Morphological and Biochemical Answer of the Wheat Seeds at Treatment with 2,4-Dinitrophenol and Potassium Iodate
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Todirascu-Ciornea,Elena; Drochioiu,Gabi; Stefanescu,Raluca; Axinte,Elena Violeta; Dumitru,Gabriela.
Oxidative stress can be regarded as an imbalance between the amount of reactive oxygen species (ROS) and the ability of a biological system to eliminate the toxic species and repair the resulting damages. Since the germinating seeds and the resulted seedlings are rich in enzymes, whereas the treatment with chemicals affects much the seed germination, producing also ROS, we evaluate here the influence of 2,4-dinitrophenol (DNP) and potassium iodate (KIO3) on wheat germination (Triticum aestivum L.) and seedlings growth. Germination rate, the masses and heights of the 7 day old seedlings, as well as the activity of some enzymes involved in the oxidative stress such as peroxidase, catalase and superoxide dismutase were measured seven days after the chemical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNP; KIO3; Oxidative stress; Wheat.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100414

Vitamin E and Sodium Selenite Against Mercuric Chloride-Induced Lung Toxicity in the Rats
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Celikoglu,Emine; Aslanturk,Ayse; Kalender,Yusuf.
The aim of the present study was to elucidate the possible protective role of vitamin E and / or sodium selenite on mercuric chloride-induced oxidative stress and histopathological changes in the lung tissue of the rats. Adult male albino Wistar rats were exposed to mercuric chloride (1.0 mg/kg day) for four weeks. Treatment with mercuric chloride led to oxidative stress by enhancing MDA level and also decreasing superoxide dismutase (SOD), catalase (CAT) glutathione peroxidase (GPx) and glutathione S transferaz (GST) activities. However, mercuric chloride exposure resulted in histopathological changes in the lung tissue in the rats. MDA level and SOD, CAT GPx and GST activities and histopathological changes modulated in concomitantly supplementation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mercuric chloride; Sodium selenite; Vitamin E; Lung toxicity; Oxidative stress; Histopathology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400587

Extract of the Bark of Bathysa cuspidataAttenuates the Development of Chemically-Induced Preneoplastic Colorectal Lesions in Rats
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Rosa,Damiana Diniz; Reis,Sandra Aparecida dos; Siqueira,Nathane Pais; Gonçalves,Reggiani Vilela; Machado,Alessandra Barbosa Ferreira; Tafuri,Natália Filardis; Leite,João Paulo Viana; Matta,Sérgio Luís Pinto da; Peluzio,Maria do Carmo Gouveia.
ABSTRACTThe aim of this study was to investigate the effect of the bark extractBathysa cuspidata on chemically induced preneoplastic colorectal lesions in Wistar rats. Forty male rats were randomly divided into four groups (n = 10 each): saline (control group, oral administration of saline solution 0.9%); dimethylsulfoxide (DMSO, vehicle control), B200 (treated with 200 mg/kg bark extract ofB. cuspidata), and B400 (treated with 400 mg/kg bark extract ofB. cuspidata). Administration of treatments was carried out by the gavage. The animals received four subcutaneous injections of 1,2-dimethylhydrazine (DMH, 40 mg/kg) in the initial two weeks of the experiment to induce preneoplastic colorectal lesions. After 15 weeks, the animals were euthanized and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bathysa cuspidata; Animal model; Oxidative stress; Aberrant crypt foci.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500732

Antioxidant Status, Lipid Peroxidation and Testis-histoarchitecture Induced by Lead Nitrate and Mercury Chloride in Male Rats
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Bas,Hatice; Kalender,Suna.
ABSTRACT This study was done to evaluate the effects of lead nitrate and mercury chloride in testis tissues of Wistar rats. Lead nitrate and mercury chloride are widely used heavy metals in industry. Oral lead and mercury administrations to adult male rats at doses 45 mg/kg bw and 0.02 mg/kg bw, respectively for 4 weeks caused a significant increasing in MDA levels and antioxidant enzyme activities (SOD, CAT, GPx and GST). The MDA levels and acivities of antioxidant enzymes was lower in rats that were administrated by lead nitrate than mercury chloride treated group. Light microscopic analyses revealed that lead nitrate and mercury chloride induced numerous histopathological changes in testis tissues of rats. Histopathological observations of the testis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant enzymes; Lead; Mercury; Oxidative stress; Testis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100341

Evaluation of Gamma Irradiation Effect on the Oxidative Stress Factors in Septic Rats Treated With Iranian Plant Essential Oils
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Fatemi,Faezeh; Yazdi,Atefeh Jalali; Ghanooni,Shirin; Zolfaghari,Mohammad Reza.
ABSTRACT Cuminum cyminum L. (CM), Zataria multiflora Boiss. (ZM) and Mentha piperita L. (MP) are traditional medicinal plants with various pharmacological properties. This study was designed to assess the role of gamma irradiation -a modern decontamination method- in hepatoprotective effects of their essential oil (E.Os) in septic rats induced by experimental cecal ligation and puncture (CLP) model. The rats were divided into 20 groups; sham-operated (SOP); CLP; CLP + CM, ZM and MP (E.Os) (100 &amp; 200 mg/kg b.w) and CLP + gamma irradiated (10 and 25 kGy) E.Os (100 &amp; 200 mg/kg b.w) as treatment groups. All E.Os were injected i.p immediately after sepsis induction. 24 hour after CLP, the rats were sacrificed and the liver tissue was examined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Essential oils; Oxidative stress; Antioxidant; CLP; Gamma irradiation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100312

Cardioprotection of Rosuvastatin Pre-conditioning on Myocardial Ischemia / Reperfusion Injury in a Rat Model
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Zhang,Pei-Xi; Zhang,Hong-Xia; Hou,Dai-Liang; Xiao,Zhi-Chao; Zhou,Cheng-Yun.
Abstract This study aimed to investigate the cardioprotection of rosuvastatin pre-conditioning (R-Pre) in a rat model of myocardial ischemia / reperfusion (I/R). Male SD rats were assigned into three groups: sham group, I/R group and R-Pre group. Rats in I/R group and R-Pre group received ischemia for 30 min and reperfusion for 2 h. In R-Pre group, rats received intragastrical administration with rosuvastatin at 5 mg/kg once daily for 1 week. After 2-h reperfusion, the cardiac function was detected by ultrasonography; the blood was collected for biochemical analysis; the heart was collected for the TUNEL staining and immunohistochemistry for Bcl-2 and Bax. Our results showed rosuvastatin pre-conditioning for 1 week could significantly reduce the infarct...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial ischemia/reperfusion; Rosuvastatin; Oxidative stress; Apoptosis; Pre-conditioning.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100311

Oxidative stress action in cellular aging
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Oliveira,Monique Cristine de; Schoffen,João Paulo Ferreira.
Various theories try to explain the biological aging by changing the functions and structure of organic systems and cells. During lifetime, free radicals in the oxidative stress lead to lipid peroxidation of cellular membranes, homeostasis imbalance, chemical residues formation, gene mutations in DNA, dysfunction of certain organelles, and the arise of diseases due to cell death and/or injury. This review describes the action of oxidative stress in the cells aging process, emphasizing the factors such as cellular oxidative damage, its consequences and the main protective measures taken to prevent or delay this process. Tests with antioxidants: vitamins A, E and C, flavonoids, carotenoids and minerals, the practice of caloric restriction and physical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Cellular aging; Cell death; Oxidative stress.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600009

Pre-eclampsia: a life-threatening pregnancy syndrom
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Brazdova,Andrea; Keprova,Alena; Zidkova,Jarmila; Madar,Jindrich.
Pre-eclampsia is a serious pathological state affecting 5-10% of pregnant women. Currently, it is diagnosed in the second half of pregnancy, particularly after the 20th week. Symptoms mostly correspond to the changes of blood vessels and kidneys. The severity of pre-eclampsia is proportional to symptomatic manifestations, thus the more symptoms present, the higher is of pre-eclampsia development. Although there are several studies dealing with pre-eclampsia pathology, the complete etiology is still unknown. In this review paper, several theories are presented and discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Placenta; Pre-eclampsia; Pregnancy; Oxidative stress.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500701

The Risk Evaluation of Tungsten Oxide Nanoparticles in Cultured Rat Liver Cells for Its Safe Applications in Nanotechnology
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Turkez,Hasan; Sonmez,Erdal; Turkez,Ozlem; Mokhtar,Yousef Ibrahem; Stefano,Antonio Di; Turgut,Guven.
Tungsten (VI) oxide (WO3) nanoparticles (NPs) are used for many industrial purposes in everyday life. However, their effects on human health have not been sufficiently evaluated. Therefore, the present study was designed to investigate the toxicity potentials of various concentrations (0 to 1000 ppm) of WO3NPs (&lt;100 nm particle size) in cultured primary rat hepatocytes. The results of cell viability assay showed that the higher concentrations of dispersed WO3 NPs (300, 500 and 1000 ppm) caused significant (p&lt;0.05) decreases of cell viability. Also, dose dependent negative alterations were observed in oxidative status and antioxidant capacity levels after the application of WO3 in cultured rat primary hepatocytes. The results of genotoxicity tests...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tungsten oxide; Nanotoxicity; Genotoxicity; Cytotoxicity; Hepatocytes; Oxidative stress.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400532

Oxidative stress parameters as biomarkers of risk factor for diabetic foot among the patients with type 2 diabetes
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Oliveira,Ana Carla Pozzi; Teixeira,Caio Jordão; Stefanello,Talitha Fernandes; Carrara,Marcia Aparecida; Bazotte,Roberto Barbosa; Sá-Nakanishi,Anacharis Babeto; Comar,Jurandir Fernando; Batista,Marcia Regina.
The aim of this study was to determine whether plasma levels of carbonylated proteins, total antioxidant capacity (TAC) and reduced protein thiols could be suitable biomarkers of risk factors for diabetic foot. Individuals with type 2 diabetes with normal protective sensation (normal foot group) vs. loss of protective sensation and/or signs of peripheral arterial disease and/or foot deformities and/or history of ulcers and/or neuropathic fractures and/or amputation (diabetic foot group) were compared. The diabetic foot group showed higher carbonylated protein levels (P = 0.0457) and lower levels of TAC (P = 0.0148) and reduced protein thiols (P = 0.0088), compared with the normal foot group. In general, several other parameters of risk of diabetes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetic foot; Oxidative stress; Total antioxidant capacity; Reduced protein thiols; Protein carbonyls.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200009

Influence of chromium salts on increased lipid peroxidation and differential pattern in antioxidant metabolism in Pistia stratiotes L
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Upadhyay,RishiKesh; Panda,Sanjib Kumar.
In this work, the changing effect of different concentrations (0, 0.01, 0.1, 1, 10mM) of hexavalent and trivalent chromium on different biochemical parameters along with antioxidant enzymes was investigated on water lettuce (Pistia stratiotes L.) in order to know the possible involvement of this metal in oxidative injury, besides the activities of antioxidant enzymes leading to biochemical and oxidative aberration induced by elevated concentrations. Both in roots and shoots, Cr produced a significant increase in enzymic and non-enzymic antioxidants, except in catalase (CAT) activity where a strong accumulation of hydrogen peroxide was indicated, suggesting an imposition of oxidative stress. The observation showed an uptake of chromium by P. stratiotes L....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biochemical alterations; Chromium stress; Lipid peroxidation; Oxidative stress; Pistia stratiotes L; Reactive oxygen species.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500018

Dry Extract of Passiflora incarnata L. leaves as a Cardiac and Hepatic Oxidative Stress Protector in LDLr-/- Mice Fed High-Fat Diet
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Sarto,Danielle Aparecida Quintino Silva; Siqueira,Ana Helena D’Arcadia de; Magalhaes,Francisco Monteiro de Almeida; Caproni,Karine de Paula; Martins,Ângela Maria; Santos,Gérsika Bitencourt; Silva,Délcio Bueno da; Boas,Brígida Monteiro Vilas; Garcia,José Antônio Dias.
ABSTRACT The control of dyslipidemia by using herbal products is an important subject for studies. In this study, we evaluated the effects of dry Passiflora incarnata L. extract over dyslipidemia, left ventricular hypertrophy, and hepatic oxidative stress of LDL receptor knockout mice (LDLr-/-). Forty 4-month old male LDLr-/- mice were distributed into four groups: Group standard diet; Group standard diet and 200 mg/kg of body weight of Passiflora incarnata L. leaf dry extract; Group high-fat diet; Group high-fat diet and 200 mg/kg of body weight of Passiflora incarnata L. leaf dry extract. After 30 days, Passiflora incarnata L. dry extract reduced the effects of the high-fat diet, with a decrease of total cholesterol, triglycerides, and increase of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passion fruit; Left ventricular hypertrophy; Dyslipidemia; Oxidative stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100321

Brassinosteroids Reciprocates Heavy Metals Induced Oxidative Stress in Radish by Regulating the Expression of Key Antioxidant Enzyme Genes
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Sharma,Indu; Sharma,Ashutosh; Pati,Pratap; Bhardwaj,Renu.
ABSTRACT Heavy metal toxicity in plants lead to accumulation of reactive oxygen species (ROS). Antioxidant enzyme system is also not able to revert altered ROS homeostasis. The present study reports the heavy metal induced ROS toxicity by up-regulating the expression of key antioxidant enzyme genes through Brassinosteroids pre-soaking treatment in radish.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brassinosteroids; Oxidative stress; Antioxidant enzymes; Gene expression.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100425

Antioxidant effect of phycocyanin on oxidative stress induced with monosodium glutamate in rats
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Bertolin,Telma Elita; Farias,Daniele; Guarienti,Cíntia; Petry,Fernanda Tais Souza; Colla,Luciane Maria; Costa,Jorge Alberto Vieira.
The objective of this work was to study the antioxidant effect of phycocyanin on the oxidative stress induced by monosodium glutamate in the rats. The tests were performed with 32 rats of Wistar breed, divided into four groups, which were administered saline solution of phycocyanin, monosodium glutamate and monosodium glutamate plus phycocyanin. Sulfhydryl groups and the secondary substances derived from lipid oxidation were determined through the level of TBA. The evaluation of these values and the level of sulfhydryl showed that the administration of phycocyanin presented significant antioxidant effect (p &lt; 0.05) reducing the oxidative stress induced by the monosodium glutamate in vivo.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Oxidative stress; Phycocyanin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400012

Hepatoprotective effects of Iranian Hypericum scabrum essential oils against oxidative stress induced by acetaminophen in rats
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Dadkhah,Abolfazl; Fatemi,Faezeh; Farsani,Mohsen Eslami; Roshanaei,Kambiz; Alipour,Mahdi; Aligolzadeh,Hadi.
This studied examined the protective role of Hypericum scabrum oils (100 and 200 mg/kg b.w, i.p) on acetaminophen-induced liver damages in the rat. The hepatic oxidative/antioxidant parameters such as lipid peroxidation (LP), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and ferric reducing ability of plasma (FRAP) were measured 2, 4, 8, 16 and 24h after the treatments confirmed by histopathological consideration. The results indicated that increased levels of hepatic LP and FRAP and SOD activity were reversed in the rats treated with oils. In addition, the depleted GSH were compensated with the oil treatments. The protective effect of the oils was further confirmed by the histophatological examination carried out on liver biopsies. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypericum scabrum; Essential oils; Acetaminophen; Rat; Oxidative stress; Liver.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300005

Lycopene Protects the Diabetic Rat Kidney Against Oxidative Stress-mediated Oxidative Damage Induced by Furan
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Pandir,Dilek; Unal,Betul; Bas,Hatice.
Furan is a food and environmental contaminant and a potent carcinogen in animals. Lycopene is one dietary carotenoid found in fruits such as tomato, watermelon and grapefruit. The present study was designed to explore the protective effect of lycopene against furan-induced oxidative damage in streptozotocin (STZ)-induced diabetic rat kidney. At the end of the experimental period (28 days), we found that lycopene markedly decreased the malondialdehide (MDA) levels in the kidney, urea, uric acid and creatinine levels in the serum of furan-treated rats. The increase of histopathology in the kidney of furan-treated rats were effectively suppressed by lycopene. Furthermore, lycopene markedly restored superoxide dismutase (SOD), catalase (CAT), glutathione...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Furan; Lycopene; Kidney; Oxidative stress; Diabetes.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100314

Evaluation of the Redox State of Wistar Rats Submitted to High-Fat Diet Supplemented With Infusion of Ilex paraguariensis
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Silva,Clédia Flores; Nardin,Patrícia; Grings,Mateus; Uecker,Julia Neitzel; Cerqueira,Jenifer Heller; Rincón,Joao Alvarado; Abib,Renata Torres; Schneider,Augusto; Barros,Carlos Castilho; Andreazza,Robson; Pieniz,Simone.
ABSTRACT This study aimed to evaluate the effect of Ilex paraguariensis infusion on redox state of Wistar rats submitted to high-fat and standard diet. Glutathione determination and lipid peroxidation in the hippocampus tissues and liver was performed, as well as the analysis of gene expression of superoxide dismutase, catalase and glutathione peroxidase by real-time polymerase chain reaction. The results from hippocampus showed that the groups fed with standard diet exhibited significant reduction of lipid peroxidation when supplemented with Ilex paraguariensis. The analysis from glutathione determination in the hippocampus showed a significant increase in glutathione activity in the group treated with high-fat diet and Ilex paraguariensis. In the liver,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ilex paraguariensis; Bioactive compounds; Oxidative stress; Antioxidant defense.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100328

Protective Effect of Carvacrol Against Oxidative Stress and Heart Injury in Cyclophosphamide-Induced Cardiotoxicity in Rat
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Cetik,Songul; Ayhanci,Adnan; Sahinturk,Varol.
Possible protective effects of carvacrol (Car) against cyclophosphamide (CP)-induced cardiotoxicity was examined in this study. Experimental groups of the rats were randomly divided into 13 groups,each including seven animals: Group 1 (control) treated with saline; groups 2, 3, and 4 treated with 50, 100, or 150 mg/kg of CP, respectively; group 5 treated with 0.5 mL olive oil; groups 6 and 7 treated with 5.0 and 10 mg/kg of Car, respectively; groups 8, 9, or 10 treated with respective CP plus 5.0 mg/kg of Car; and groups 11, 12, or 13 treated with respective CP plus 10 mg/kg of Car. Serum alanine transaminase (ALT),aspartat transaminase (AST), lactate dehydrogenase (LDH), malondialdehyde (MDA),creatine kinase-MB (CK-MB), total oxidant state (TOS),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclophosphamide; Oxidative stress; Cardiotoxicity; Carvacrol; Antioxidant; Rat.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400569

Evaluation of lipid profile and oxidative stress in STZ-induced rats treated with antioxidant vitamin
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Almeida,Danielle Ayr Tavares de; Braga,Camila Pereira; Novelli,Ethel Lourenzi Barbosa; Fernandes,Ana Angélica Henrique.
The present study investigated the effect of supplementation of vitamin E on streptozotocin (STZ)-induced diabetic rats by measuring blood glucose, changes in body weight, food and water intake, lipid profile, serum urea and creatinine level, and antioxidant enzyme activity. Male Wistar rats were divided into four groups: control rats (GI); rats receiving vitamin E (GII); STZ-induced diabetic rats (GIII) and STZ-induced diabetic rats treated with vitamin E (GIV). Vitamin E reduced (p&lt;0.05) blood glucose and urea, improved the lipid profile (decreased the serum levels of total cholesterol, LDL cholesterol, VLDL cholesterol and triacylglycerols, and increased HDL cholesterol) and increased total protein in STZ-induced diabetic rats (GIV). Vitamin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitamin E; Lipid profile; Oxidative stress; STZ.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400007

Antioxidant Effects of Biochanin A in Streptozotocin Induced Diabetic Rats
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Sadri,Hamideh; Goodarzi,Mohammad Taghi; Salemi,Zahra; Seifi,Morteza.
ABSTRACT Bioflavonoid-containing diets have been reported to be beneficial in diabetes. In the current study, the effect of Biochanin A (BCA) on blood glucose, antioxidant enzyme activities and oxidative stress markers in diabetic rats were investigated. 30 male Wistar rats were divided into five groups. Two of them were selected as control; group1: control (receiving 0.5%DMSO), and group2: Control+BCA (receiving 10 mg/kg.bw BCA). Diabetes was induced in other rats with injection of (55 mg/kg.bw) streptozotocin; group3: diabetic control (receiving 0.5%DMSO), groups 4 and 5 were treated with 10 and 15 mg/kg.bw BCA respectively. After 6 weeks the following results were obtained. Fasting blood glucose (FBG), Triglyceride (TG), total cholesterol (TC), low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biochanin A; Diabetic rats; Oxidative stress; STZ.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100313

In Vitro Study Towards Role of Acrylamide-Induced Genotoxicity in Human Lymphocytes and the Protective Effect of L- Carnitine
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Zamani,Ehsan; Shokrzadeh,Mohammad; Modanloo,Mona; Shaki,Fatemeh.
ABSTRACT Acrylamide (AA), is an important industrial compound, formed during food processing under high temperature. Due to its potential carcinogenic effect, it has been recognized as a human health concern. In this study, we investigated the role of oxidative stress in acrylamide’s genotoxicity and protective role of L- carnitine (LCA) in human lymphocytes. The micronucleus test (MNT) was used to monitor AA genotoxicity after 20h exposure to concentrations of 0.25,50 and 100 mM. Furthermore we evaluated oxidative stress markers, such as reactive oxygen species (ROS), lipid peroxidation (LPO) and glutathione (GSH) content. In MNT, It has been found that AA at high concentrations (50 and 100 µM) significantly increased the micronuclei (MN) frequencies. On...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acrylamide; L- carnitine; Micronucleus; Oxidative stress; Genotoxicity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100426

Biomarkers of oxidative stress and acetylcholinesterase activity in the blood of grass snake (Natrix natrix L.) during prehibernation and posthibernation periods
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Gavric,Jelena; Prokic,Marko; Despotovic,Svetlana; Gavrilovic,Branka; Radovanovic,Tijana; Borkovic-Mitic,Slavica; Pavlovic,Sladan; Saičic,Zorica.
This work examined the enzymatic (superoxide dismutase-CuZn SOD, catalase-CAT, glutathione peroxidase-GSHPx, glutathione reductase-GR, and the biotransformation phase II enzyme glutathione-S-transferase-GST) and nonenzymatic (total glutathione-GSH and lipid peroxides-TBARS concentrations) biomarkers of oxidative stress and acetylcholinesterase (AChE) activity in the blood of the grass snake (Natrix natrix L.) during prehibernation and posthibernation. The animals were collected in October (prehibernation) and April (posthibernation) at the nature reserve Obedska Bara (OB) and industrial region Pancevacki Rit (PR) in Serbia. In posthibernation, decreased CAT activity and TBARS concentration in specimens from PR, and decreased GR and AChE activities, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Grass snake; Oxidative stress; Hibernation; Acetylcholinesterase; Antioxidant enzymes.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300443

Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Oliveira,Yanaihara Pinchemel Amorim de; Pontes-de-Carvalho,Lain Carlos; Couto,Ricardo David; Noronha-Dutra,Alberto Augusto.
Abstract  Background: Sepsis is an illness with a high morbidity for which no effective treatment exists. Its treatment has a high cost because it usually requires an intensive care unit and expensive antibiotics. The present study focus in the production of reactive oxygen species in the early stages of sepsis. This study aimed at investigating the production of reactive oxygen specie during the inflammatory response in patients with sepsis.  Methods: Reactive oxygen specie production and insoluble myeloperoxidase obtained from fresh whole blood were measured by photon counting chemiluminescence in the blood of 18 septic patients and 12 healthy individuals. Modified red blood cells were evaluated by staining of blood smears. The production of reactive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sepsis; Oxidative stress; Inflammation; Modified erythrocytes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100019

Assessment of antioxidants status and superoxide dismutase activity in HIV-infected children
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Pugliese,Camila; Patin,Rose Vega; Palchetti,Cecilia Zanin; Claudio,Cristiane Chiantelli; Gouvêa,Aída de Fátima Thomé Barbosa; Succi,Regina Célia de Menezes; Amancio,Olga Maria Silverio; Cozzolino,Silvia Maria Franciscato; Oliveira,Fernanda Luisa Ceragioli.
Objective:This study aims to assess the nutritional status of selenium, copper and zinc; and also the erythrocyte superoxide dismutase activity of HIV-infected children compared to a control group.Methods:A cross-sectional study was carried out with prepubertal HIV-infected children (n = 51) and their healthy siblings (n = 32). All biochemical measurements including plasma selenium, serum copper levels, serum and erythrocyte zinc levels and erythrocyte super-oxide dismutase activity were evaluated according to dietary, clinical and biochemical parameters.Results:Compared to the control group, the HIV-infected children had lower z-score values for height-for-age (p = 0.0006), higher prevalence of stunting (11.8%) (p = 0.047), lower selenium levels (p =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; HIV and nutrition; Oxidative stress; Trace elements.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000500481

Evaluation of oxidative status in patients with brucellosis
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Serefhanoglu,Kivanc; Taskin,Abdullah; Turan,Hale; Timurkaynak,Funda Ergin; Arslan,Hande; Erel,Ozcan.
Oxidative stress can be defined as an increase in oxidants and/or a decrease in antioxidant capacity. We aimed to determine total antioxidant capacity (TAC), total peroxide, malondialdehyde and catalase levels in plasma samples, and calculation of oxidative stress index (OSI) in patients with brucellosis to evaluate their oxidative status using a novel automated method. Sixty-nine patients with brucellosis and 69 healthy control subjects were included in the present study. Plasma levels of total peroxide and malondialdehyde were significantly increased in patients as compared with healthy controls (p&lt;0.001 and p&lt;0.001, respectively). In contrast, TAC level was significantly lower in patients as compared with controls (p&lt;0.001). There was no...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Brucellosis; Oxidants; Antioxidants; Oxidative stress.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000400001

Effect of (-)-∆9-tetrahydrocannabinoid on the hepatic redox state of mice
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Pinto,C.E.; Moura,E.; Serrão,M.P.; Martins,M.J.; Vieira-Coelho,M.A.
(-)-∆9-Tetrahydrocannabinol (∆9-THC), a psychoactive component of marijuana, has been reported to induce oxidative damage in vivo and in vitro. In this study, we administered ∆9-THC to healthy C57BL/6J mice aged 15 weeks in order to determine its effect on hepatic redox state. Mice were divided into 3 groups: ∆9-THC (N = 10), treated with 10 mg/kg body weight ∆9-THC daily; VCtrl (N = 10), treated with vehicle [1:1:18, cremophor EL® (polyoxyl 35 castor oil)/ethanol/saline]; Ctrl (N = 10), treated with saline. Animals were injected ip twice a day with 5 mg/kg body weight for 10 days. Lipid peroxidation, protein carbonylation and DNA oxidation were used as biomarkers of oxidative stress. The endogenous antioxidant defenses analyzed were glutathione (GSH)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: (-)-∆9-Tetrahydrocannabinol; Oxidative stress; Liver; Cannabinoid receptors; Cremophor EL®.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400001

Preeclampsia: from epidemiological observations to molecular mechanisms
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: López-Jaramillo,P.; Casas,J.P.; Serrano,N.
Preeclampsia is the main cause of maternal mortality and is associated with a five-fold increase in perinatal mortality in developing countries. In spite of this, the etiology of preeclampsia is unknown. The present article analyzes the contradictory results of the use of calcium supplementation in the prevention of preeclampsia, and tries to give an explanation of these results. The proposal of an integrative model to explain the clinical manifestations of preeclampsia is discussed. In this proposal we suggest that preeclampsia is caused by nutritional, environmental and genetic factors that lead to the creation of an imbalance between the free radicals nitric oxide, superoxide and peroxynitrate in the vascular endothelium. The adequate interpretation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Preeclampsia; Calcium supplementation; Nitric oxide; Infection; Inflammation; Oxidative stress; Endothelial dysfunction.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000001

Acute effects of resistance exercise and intermittent intense aerobic exercise on blood cell count and oxidative stress in trained middle-aged women
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Cardoso,A.M.; Bagatini,M.D.; Roth,M.A.; Martins,C.C.; Rezer,J.F.P.; Mello,F.F.; Lopes,L.F.D.; Morsch,V.M.; Schetinger,M.R.C.
The aim of this study was to compare the effect of an intermittent intense aerobic exercise session and a resistance exercise session on blood cell counts and oxidative stress parameters in middle-aged women. Thirty-four women were selected and divided into three groups: RE group (performing 60 min of resistance exercises, N = 12), spinning group (performing 60 min of spinning, N = 12), and control group (not exercising regularly, N = 10). In both exercise groups, lymphocytes and monocytes decreased after 1-h recuperation (post-exercise) compared to immediately after exercise (P &lt; 0.05). Immediately after exercise, in both exercised groups, a significant increase in TBARS (from 16.5 ± 2 to 25 ± 2 for the spinning group and from 18.6 ± 1 to 28.2 ± 3 nmol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intermittent exercise; Anaerobic exercise; Oxidative stress; Immune suppression; Middle-aged women.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200010

Angiotensin-(1–7) inhibits inflammation and oxidative stress to relieve lung injury induced by chronic intermittent hypoxia in rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Lu,W.; Kang,J.; Hu,K.; Tang,S.; Zhou,X.; Yu,S.; Li,Y.; Xu,L.
Obstructive sleep apnea is associated with inflammation and oxidative stress in lung tissues and can lead to metabolic abnormalities. We investigated the effects of angiotensin1–7 [Ang-(1–7)] on lung injury in rats induced by chronic intermittent hypoxia (CIH). We randomly assigned 32 male Sprague-Dawley rats (180–200 g) to normoxia control (NC), CIH-untreated (uCIH), Ang-(1–7)-treated normoxia control (N-A), and Ang-(1–7)-treated CIH (CIH-A) groups. Oxidative stress biomarkers were measured in lung tissues, and expression of NADPH oxidase 4 (Nox4) and Nox subunits (p22phox, and p47phox) was determined by Western blot and reverse transcription-polymerase chain reaction. Pulmonary pathological changes were more evident in the uCIH group than in the other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ang-(1–7); Lung injury; Obstructive sleep apnea; Inflammation; Oxidative stress; Chronic intermittent hypoxia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000603

Ilex paraguariensis extracts extend the lifespan of Drosophila melanogaster fed a high-fat diet
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Colpo,A.C.; Lima,M.E.; da Rosa,H.S.; Leal,A.P.; Colares,C.C.; Zago,A.C.; Salgueiro,A.C.F.; Bertelli,P.R.; Minetto,L.; Moura,S.; Mendez,A.S.L.; Folmer,V.
Studies have suggested that total energy intake and diet composition affect lifespan and ageing. A high-fat diet induces oxidative stress and affects the development of diseases. In contrast, antioxidants are capable of reducing its harmful effects. Yerba mate beverages are an important source of antioxidants, but there is scarce knowledge about their effects on suppressing fat accumulation. Here, we investigated the compounds present in yerba mate extracts and assessed their effects on Drosophila melanogaster given a high cholesterol diet. LS-ESI-MS analysis showed the presence of matesaponins, phenolic compounds and methylxanthines in all of the examined extracts. In Drosophila, under extract treatment conditions, the mean lifespan was significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yerba mate; Matesaponin; LC-MS; Antioxidants compounds; Chronic diseases; Oxidative stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200601

Time-course effects of aerobic exercise training on cardiovascular and renal parameters in 2K1C renovascular hypertensive rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Maia,R.C.A.; Sousa,L.E.; Santos,R.A.S.; Silva,M.E.; Lima,W.G.; Campagnole-Santos,M.J.; Alzamora,A.C.
Exercise training (Ex) has been recommended for its beneficial effects in hypertensive states. The present study evaluated the time-course effects of Ex without workload on mean arterial pressure (MAP), reflex bradycardia, cardiac and renal histology, and oxidative stress in two-kidney, one-clip (2K1C) hypertensive rats. Male Fischer rats (10 weeks old; 150–180 g) underwent surgery (2K1C or SHAM) and were subsequently divided into a sedentary (SED) group and Ex group (swimming 1 h/day, 5 days/week for 2, 4, 6, 8, or 10 weeks). Until week 4, Ex decreased MAP, increased reflex bradycardia, prevented concentric hypertrophy, reduced collagen deposition in the myocardium and kidneys, decreased the level of thiobarbituric acid-reactive substances (TBARS) in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2K1C renovascular hypertension; Swimming; Baroreflex bradycardia; Heart and kidney adaptations; Oxidative stress.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001101010

Effect of eccentric training on mitochondrial function and oxidative stress in the skeletal muscle of rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Silva,L.A.; Bom,K.F.; Tromm,C.B.; Rosa,G.L.; Mariano,I.; Pozzi,B.G.; Tuon,T.; Stresck,E.L.; Souza,C.T.; Pinho,R.A.
The objective of the present study was to investigate the effects of eccentric training on the activity of mitochondrial respiratory chain enzymes, oxidative stress, muscle damage, and inflammation of skeletal muscle. Eighteen male mice (CF1) weighing 30-35 g were randomly divided into 3 groups (N = 6): untrained, trained eccentric running (16°; TER), and trained running (0°) (TR), and were submitted to an 8-week training program. TER increased muscle oxidative capacity (succinate dehydrogenase and complexes I and II) in a manner similar to TR, and TER did not decrease oxidative damage (xylenol and creatine phosphate) but increased antioxidant enzyme activity (superoxide dismutase and catalase) similar to TR. Muscle damage (creatine kinase) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eccentric training; Mitochondrial enzyme activity; Oxidative stress; Muscle damage; Inflammation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100014

Physical exercise prevents motor disorders and striatal oxidative imbalance after cerebral ischemia-reperfusion
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Sosa,P.M.; Schimidt,H.L.; Altermann,C.; Vieira,A.S.; Cibin,F.W.S.; Carpes,F.P.; Mello-Carpes,P.B.
Stroke is the third most common cause of death worldwide, and most stroke survivors present some functional impairment. We assessed the striatal oxidative balance and motor alterations resulting from stroke in a rat model to investigate the neuroprotective role of physical exercise. Forty male Wistar rats were assigned to 4 groups: a) control, b) ischemia, c) physical exercise, and d) physical exercise and ischemia. Physical exercise was conducted using a treadmill for 8 weeks. Ischemia-reperfusion surgery involved transient bilateral occlusion of the common carotid arteries for 30 min. Neuromotor performance (open-field and rotarod performance tests) and pain sensitivity were evaluated beginning at 24 h after the surgery. Rats were euthanized and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stroke; Striatum; Locomotion; Oxidative stress; Antioxidants; Running.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900798

Superoxide: a two-edged sword
Provedor de dados: Brazilian Journal of Medical and Biological Research
Superoxide (O2-) is the compound obtained when oxygen is reduced by one electron. For a molecule with an unpaired electron, O2- is surprisingly inert, its chief reaction being a dismutation in which it reacts with itself to form H2O2 and oxygen. The involvement of O2- in biological systems was first revealed by the discovery in 1969 of superoxide dismutase, an enzyme that catalyzes the dismutation of O2-. Since then it has been found that biological systems produce a bewildering variety of reactive oxidants, all but a few arising ultimately from O2-. These oxidants include O2- itself, H2O2 and alkyl peroxides, hydroxyl radical and other reactive oxidizing radicals, oxidized halogens and halamines, singlet oxygen, and peroxynitrite. These various oxidants...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: NADPH oxidase; Superoxide; Oxidative stress; Antioxidant; Regulation; Host defense.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200001

Inhibition of miR-383 suppresses oxidative stress and improves endothelial function by increasing sirtuin 1
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Hu,Baoxiang; Gong,Zushun; Bi,Zhaohui.
Previous research has shown that suppression of miR-383 can prevent inflammation of the endothelium, as well as postpone the development of atherosclerosis. However, the role of miR-383 in endothelial cell apoptosis in diabetes remains unclear. The aim of this study was to investigate the function of miR-383 in high glucose-induced apoptosis and oxidative stress in endothelial cells. A series of experiments involving qualitative polymerase chain reaction, cell transfection, luciferase assay, assessment of cell death, detection of catalase and superoxide dismutase concentrations, detection of intracellular reactive oxygen species (ROS), and western blot analysis were performed in this study. We found that miR-383 expression was promoted, while...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiR-383; Oxidative stress; Human umbilical vein endothelial cells; Sirtuin 1.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200603

Lactate-upregulation of lactate oxidation complex-related genes is blunted in left ventricle of myocardial infarcted rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Gabriel-Costa,D.; Cunha,T.F.; Paixão,N.A.; Fortunato,R.S.; Rego-Monteiro,I.C.C.; Barreto-Chaves,M.L.M.; Brum,P.C.
Lactate modulates the expression of lactate oxidation complex (LOC)-related genes and cardiac blood flow under physiological conditions, but its modulatory role remains to be elucidated regarding pathological cardiac stress. The present study evaluated the effect of lactate on LOC-related genes expression and hemodynamics of hearts submitted to myocardial infarction (MI). Four weeks after MI or sham operation, isolated hearts of male Wistar rats were perfused for 60 min with Na+-lactate (20 mM). As expected, MI reduced cardiac contractility and relaxation with no changes in perfusion. The impaired cardiac hemodynamics were associated with increased reactive oxygen species (ROS) levels (Sham: 19.3±0.5 vs MI: 23.8±0.3 µM), NADPH oxidase (NOX) activity (Sham:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactate; Lactate oxidation complex; Myocardial infarction; Gene expression; Perfusion pressure; Oxidative stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100605

Methylmercury inhibits prolactin release in a cell line of pituitary origin
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Maués,L.A.L.; Macchi,B.M.; Crespo-López,M.E.; Nasciutti,L.E.; Picanço-Diniz,D.L.W.; Antunes-Rodrigues,J.; Nascimento,J.L.M. do.
Heavy metals, such as methylmercury, are key environmental pollutants that easily reach human beings by bioaccumulation through the food chain. Several reports have demonstrated that endocrine organs, and especially the pituitary gland, are potential targets for mercury accumulation; however, the effects on the regulation of hormonal release are unclear. It has been suggested that serum prolactin could represent a biomarker of heavy metal exposure. The aim of this study was to evaluate the effect of methylmercury on prolactin release and the role of the nitrergic system using prolactin secretory cells (the mammosomatotroph cell line, GH3B6). Exposure to methylmercury (0-100 μM) was cytotoxic in a time- and concentration-dependent manner, with an LC50...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mercury; Methylmercury; Prolactin; Oxidative stress; Pituitary; Nitric oxide.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800691

Antioxidant status of dog aqueous humor after extracapsular lens extraction
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Barros,P.S.M.; Padovani,C.F.; Silva,V.V.; Queiroz,L.; Barros,S.B.M.
We determined the antioxidant status of the aqueous humor after extracapsular lens extraction in 14 mongrel dogs weighing about 10 kg. The animals were examined by slit lamp biomicroscopy, applanation tonometry and indirect ophthalmoscopy. One eye was submitted to conventional extracapsular lens extraction and the other was used as control. Samples of aqueous humor were obtained by anterior chamber paracentesis before and at days 1, 2, 3, 7 and 15 after surgery. Total antioxidant status was determined as the capacity of aqueous humor to inhibit free radical generation by 2,2-azobis(2-amidopropane) chlorine. Ascorbic acid concentration was measured by HPLC with UV detection. Protein content was determined with the biuret reagent. Statistical analysis was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lens; Aqueous humor; Oxidative stress; Antioxidant; Extracapsular lens extraction.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100007

Oxidative stress in the latissimus dorsi muscle of diabetic rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: De Angelis,K.L.D.; Cestari,I.A.; Barp,J.; Dall'Ago,P.; Fernandes,T.G.; Homem de Bittencourt,P.I.; Belló-Klein,A.; Belló,A.A.; Llesuy,S.; Irigoyen,M.C.
The purpose of the present study was to investigate the effects of experimental diabetes on the oxidant and antioxidant status of latissimus dorsi (LD) muscles of male Wistar rats (220 ± 5 g, N = 11). Short-term (5 days) diabetes was induced by a single injection of streptozotocin (STZ, 50 mg/kg, iv; glycemia &gt;300 mg/dl). LD muscle of STZ-diabetic rats presented higher levels of thiobarbituric acid reactive substances (TBARS) and chemiluminescence (0.36 ± 0.02 nmol/mg protein and 14706 ± 1581 cps/mg protein) than LD muscle of normal rats (0.23 ± 0.04 nmol/mg protein and 7389 ± 1355 cps/mg protein). Diabetes induced a 92% increase in catalase and a 27% increase in glutathione S-transferase activities in LD muscle. Glutathione peroxidase activity was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Streptozotocin-diabetes; Latissimus dorsi muscle; Oxidative stress; Catalase; Glutathione.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100016

Ambient particulate air pollution from vehicles promotes lipid peroxidation and inflammatory responses in rat lung
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Pereira,C.E.L.; Heck,T.G.; Saldiva,P.H.N.; Rhoden,C.R.
Oxidative stress plays a major role in the pathogenesis of particle-dependent lung injury. Ambient particle levels from vehicles have not been previously shown to cause oxidative stress to the lungs. The present study was conducted to a) determine whether short-term exposure to ambient levels of particulate air pollution from vehicles elicits inflammatory responses and lipid peroxidation in rat lungs, and b) determine if intermittent short-term exposures (every 4 days) induce some degree of tolerance. Three-month-old male Wistar rats were exposed to ambient particulate matter (PM) from vehicles (N = 30) for 6 or 20 continuous hours, or for intermittent (5 h) periods during 20 h for 4 consecutive days or to filtered air (PM &amp;lt;10 µm; N = 30). Rats...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Reactive oxygen species; Lipid peroxidation; Particulate air pollution; Lung inflammation; Acute effects.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000008

Pancreatic nitric oxide and oxygen free radicals in the early stages of streptozotocin-induced diabetes mellitus in the rat
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: González,E.; Roselló-Catafau,J.; Jawerbaum,A.; Sinner,D.; Pustovrh,C.; Vela,J.; White,V.; Xaus,C.; Peralta,C.; Gimeno,M.
The objective of the present study was to explore the regulatory mechanisms of free radicals during streptozotocin (STZ)-induced pancreatic damage, which may involve nitric oxide (NO) production as a modulator of cellular oxidative stress. Removal of oxygen species by incubating pancreatic tissues in the presence of polyethylene glycol-conjugated superoxide dismutase (PEG-SOD) (1 U/ml) produced a decrease in nitrite levels (42%) and NO synthase (NOS) activity (50%) in diabetic but not in control samples. When NO production was blocked by N G-monomethyl-L-arginine (L-NMMA) (600 µM), SOD activity increased (15.21 ± 1.23 vs 24.40 ± 2.01 U/mg dry weight). The increase was abolished when the NO donor, spermine nonoate, was added to the incubating medium (13.2 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptozotocin diabetes; Pancreas; Oxidative stress; Nitric oxide.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100012

Swimming training attenuates oxidative damage and increases enzymatic but not non-enzymatic antioxidant defenses in the rat brain
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Nonato,L.F.; Rocha-Vieira,E.; Tossige-Gomes,R.; Soares,A.A.; Soares,B.A.; Freitas,D.A.; Oliveira,M.X.; Mendonça,V.A.; Lacerda,A.C.; Massensini,A.R.; Leite,H.R.
Although it is well known that physical training ameliorates brain oxidative function after injuries by enhancing the levels of neurotrophic factors and oxidative status, there is little evidence addressing the influence of exercise training itself on brain oxidative damage and data is conflicting. This study investigated the effect of well-established swimming training protocol on lipid peroxidation and components of antioxidant system in the rat brain. Male Wistar rats were randomized into trained (5 days/week, 8 weeks, 30 min; n=8) and non-trained (n=7) groups. Forty-eight hours after the last session of exercise, animals were euthanized and the brain was collected for oxidative stress analysis. Swimming training decreased thiobarbituric acid reactive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise therapy; Oxidative stress; Rat; Lipid peroxidation; Superoxide dismutase; Swimming training.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000605

Mechanisms of endothelial dysfunction in obesity-associated hypertension
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Lobato,N.S.; Filgueira,F.P.; Akamine,E.H.; Tostes,R.C.; Carvalho,M.H.C.; Fortes,Z.B.
Obesity is strongly associated with high blood pressure, dyslipidemia, and type 2 diabetes. These conditions synergistically increase the risk of cardiovascular events. A number of central and peripheral abnormalities can explain the development or maintenance of high blood pressure in obesity. Of great interest is endothelial dysfunction, considered to be a primary risk factor in the development of hypertension. Additional mechanisms also related to endothelial dysfunction have been proposed to mediate the development of hypertension in obese individuals. These include: increase in both peripheral vasoconstriction and renal tubular sodium reabsorption, increased sympathetic activity and overactivation of both the renin-angiotensin system and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Obesity; Endothelial dysfunction; Oxidative stress; Renin-angiotensin system; Nitric oxide.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500003

Therapeutic concentration of morphine reduces oxidative stress in glioma cell line
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Almeida,M.B.; Costa-Malaquias,A.; Nascimento,J.L.M.; Oliveira,K.R.; Herculano,A.M.; Crespo-López,M.E.
Morphine is a potent analgesic opioid used extensively for pain treatment. During the last decade, global consumption grew more than 4-fold. However, molecular mechanisms elicited by morphine are not totally understood. Thus, a growing literature indicates that there are additional actions to the analgesic effect. Previous studies about morphine and oxidative stress are controversial and used concentrations outside the range of clinical practice. Therefore, in this study, we hypothesized that a therapeutic concentration of morphine (1 μM) would show a protective effect in a traditional model of oxidative stress. We exposed the C6 glioma cell line to hydrogen peroxide (H2O2) and/or morphine for 24 h and evaluated cell viability, lipid peroxidation, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphine; Glia; Oxidative stress; Hydrogen peroxide; Lipid peroxidation; Opiod.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500398

Does whole body vibration exercise improve oxidative stress markers in women with fibromyalgia?
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Santos,J.M.; Mendonça,V.A.; Ribeiro,V.G.C.; Tossige-Gomes,R.; Fonseca,S.F.; Prates,A.C.N.; Flor,J.; Oliveira,A.C.C.; Martins,J.B.; Garcia,B.C.C.; Leite,H.R.; Figueiredo,P.H.S.; Bernardo-Filho,M.; Lacerda,A.C.R.
The objective of this study was to investigate the effect of whole body vibration (WBV) exercise on oxidative stress markers in a group of women with fibromyalgia (FM) compared to a group of healthy women (CT). Twenty-one women diagnosed with FM and 21 age- and weight-matched healthy women were enrolled the study. Plasma oxidative stress markers (primary outcomes) were evaluated at rest and after WBV, and included thiobarbituric acid reactive substances (TBARS), iron reduction capacity (FRAP), superoxide dismutase antioxidant enzymes activity (SOD), and catalase (CAT). At rest, the FM group had higher TBARS (P&lt;0.001) and FRAP (P&lt;0.001), and lower CAT (P=0.005) compared to the CT. In the CT group, the WBV had no effect on TBARS (P=0.559) and FRAP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Whole body vibration; Chronic disease; Oxidative stress; Fibromyalgia.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000800609

The inflammatory stimulus of a natural latex biomembrane improves healing in mice
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Andrade,T.A.M.; Iyer,A.; Das,P.K.; Foss,N.T.; Garcia,S.B.; Coutinho-Netto,J.; Jordão-Jr.,A.A.; Frade,M.A.C.
The aim of the present study was to compare healing obtained with biomembranes with the natural healing process (sham) using biochemical and immunohistological assays. C57BL/6 mice were divided into 4 groups of 15 mice each and received different subcutaneous implants: natural latex biomembrane (NLB), denatured latex (DL), expanded polytetrafluorethylene (ePTFE), or sham. On the 2nd, 7th, and 14th days post-treatment, 5 mice per group were sacrificed and biopsied for the following measurements: oxidative stress based on malondialdehyde (MDA), myeloperoxidase (MPO) and hydrogen peroxide by the method of ferrous oxidation-xylenol orange (FOX), as well as glutathione and total proteins; histological evaluation to enumerate inflammatory cells, fibroblasts,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wound healing; Inflammation; Oxidative stress; Angiogenesis; Latex; Histomorphometry.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000009

Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Nunes,V.A.; Gozzo,A.J.; Juliano,M.A.; Cerqueira César,M.; Sampaio,M.U.; Sampaio,C.A.M.; Araújo,M.S.
Apoptosis and necrosis are two distinct forms of cell death that can occur in response to different agents and stress conditions. In order to verify if the oxidative stress induced by dietary selenium and vitamin E deficiencies can lead muscle cells to apoptosis, one-day-old chicks were reared using diets differing in their vitamin E (0 or 10 IU/kg) and selenium (0 or 0.15 ppm) supplementation. Chick skeletal muscle tissue was obtained from 28-day-old animals and used to verify apoptosis occurrence based on caspase activity detection and DNA fragmentation. Antioxidant deficiency significantly increased caspase-like activity assessed by the hydrolysis of fluorogenic peptide substrates (Abz-peptidyl-EDDnp) at lambdaexc = 320 nm and lambdaem = 420 nm....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Caspase activation; Apoptosis; Dietary selenium deficiency; Dietary vitamin E deficiency; Internucleosomal DNA fragmentation; Caspase substrate.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800010

Moderate-intensity exercise allows enhanced protection against oxidative stress-induced cardiac dysfunction in spontaneously hypertensive rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Mi,Chunjuan; Qin,Xinghua; Hou,Zuoxu; Gao,Feng.
The progression of myocardial injury secondary to hypertension is a complex process related to a series of physiological and molecular factors including oxidative stress. This study aimed to investigate whether moderate-intensity exercise (MIE) could improve cardiac function and oxidative stress in spontaneously hypertensive rats (SHRs). Eight-week-old male SHRs and age-matched male Wistar-Kyoto rats were randomly assigned to exercise training (treadmill running at a speed of 20 m/min for 1 h continuously) or kept sedentary for 16 weeks. Cardiac function was monitored by polygraph; cardiac mitochondrial structure was observed by scanning electron microscope; tissue free radical production was measured using dihydroethidium staining. Expression levels of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise training; SHR; Oxidative stress; SIRT3/SOD2; Mitochondria function.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000600603

Inflammatory and oxidative stress airway markers in premature newborns of hypertensive mothers
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Madoglio,R.J.; Rugolo,L.M.S.S.; Kurokawa,C.S.; Sá,M.P.A.; Lyra,J.C.; Antunes,L.C.O.
Although oxidative stress and inflammation are important mechanisms in the pathophysiology of preeclampsia and preterm diseases, their contribution to the respiratory prognosis of premature infants of hypertensive mothers is not known. Our objective was to determine the levels of oxidative stress and inflammation markers in the airways of premature infants born to hypertensive and normotensive mothers, in the first 72 h of life, and to investigate whether they are predictors of bronchopulmonary dysplasia (BPD)/death. This was a prospective study with premature infants less than 34 weeks’ gestation on respiratory support who were stratified into 2 groups: 32 premature infants of hypertensive mothers and 41 of normotensive women, with a mean gestational age...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infant; Premature; Pre-eclampsia; Oxidative stress; Cytokines; Bronchopulmonary dysplasia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900703

Involvement of glutamate and reactive oxygen species in methylmercury neurotoxicity
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Aschner,M.; Syversen,T.; Souza,D.O.; Rocha,J.B.T.; Farina,M.
This review addresses the mechanisms of methylmercury (MeHg)-induced neurotoxicity, specifically examining the role of oxidative stress in mediating neuronal damage. A number of critical findings point to a central role for astrocytes in mediating MeHg-induced neurotoxicity as evidenced by the following observations: a) MeHg preferentially accumulates in astrocytes; b) MeHg specifically inhibits glutamate uptake in astrocytes; c) neuronal dysfunction is secondary to disturbances in astrocytes. The generation of reactive oxygen species (ROS) by MeHg has been observed in various experimental paradigms. For example, MeHg enhances ROS formation both in vivo (rodent cerebellum) and in vitro (isolated rat brain synaptosomes), as well as in neuronal and mixed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methylmercury neurotoxicity; Oxidative stress; Glutamate and selenocompounds; Reactive oxygen species; Astrocytes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300001

Baicalin ameliorates chronic mild stress-induced depression-like behaviors in mice and attenuates inflammatory cytokines and oxidative stress
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhong,Juying; Li,Gonghua; Xu,Hong; Wang,Yan; Shi,Mingming.
The natural flavonoid glycoside baicalin (BA) produces a variety of pharmaceutical effects, particularly for psychiatric/neurological disorders. This study evaluated the behavioral and neuroprotective effects of BA in mice subjected to chronic unpredictable mild stress, a model of depression. BA (25 and 50 mg/kg) significantly increased sucrose consumption and reduced immobility times in the tail suspension and forced swim tests, demonstrating that BA alleviated depression-like behaviors. Moreover, BA reduced the levels of inflammatory cytokines, such as interleukin 1β, interleukin 6, and tumor necrosis factor α, in serum and in the hippocampus. BA also abrogated increases in NMDAR/NR2B and Ca2+/calmodulin-dependent protein kinase II, and the decrease in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baicalin; Depression; Neuroinflammatory; Oxidative stress.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700602

Attenuation of phosphamidon-induced oxidative stress and immune dysfunction in rats treated with N-acetylcysteine
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Suke,S.G.; Ahmed,R.S.; Pathak,R.; Tripathi,A.K.; Banerjee,B.D.
The effect of N-acetylcysteine, a thiolic antioxidant, on attenuation of phosphamidon-induced oxidative stress and immune dysfunction was evaluated in adult male Wistar rats weighing 200-250 g. Rats were divided into four groups, 8 animals/group, and treated with phosphamidon, N-acetylcysteine or the combination of both for 28 days. Oral administration of phosphamidon (1.74 mg/kg), an organophosphate insecticide, increased serum malondialdehyde (3.83 ± 0.18 vs 2.91 ± 0.24 nmol/mL; P &lt; 0.05) and decreased erythrocyte superoxide dismutase (567.8 ± 24.36 vs 749.16 ± 102.61 U/gHb; P &lt; 0.05), catalase activity (1.86 ± 0.18 vs 2.43 ± 0.08 U/gHb; P &lt; 0.05) and whole blood glutathione levels (1.25 ± 0.21 vs 2.28 ± 0.08 mg/gHb; P &lt; 0.05) showing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Antioxidant; Organophosphate; Oxidative stress; Immunotoxicity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900004

Lipoic acid, but not tempol, preserves vascular compliance and decreases medial calcification in a model of elastocalcinosis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Bassi,E.; Liberman,M.; Martinatti,M.K.; Bortolotto,L.A.; Laurindo,F.R.M.
Vascular calcification decreases compliance and increases morbidity. Mechanisms of this process are unclear. The role of oxidative stress and effects of antioxidants have been poorly explored. We investigated effects of the antioxidants lipoic acid (LA) and tempol in a model of atherosclerosis associated with elastocalcinosis. Male New Zealand white rabbits (2.5-3.0 kg) were fed regular chow (controls) or a 0.5% cholesterol (chol) diet+104 IU/day vitamin D2 (vitD) for 12 weeks, and assigned to treatment with water (vehicle, n=20), 0.12 mmol·kg-1·day-1 LA (n=11) or 0.1 mmol·kg-1·day-1 tempol (n=15). Chol+vitD-fed rabbits developed atherosclerotic plaques associated with expansive remodeling, elastic fiber disruption, medial calcification, and increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Vascular calcification; Atherosclerosis; Oxidative stress; Lipoic acid.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200119

Effect of Ginkgo biloba on the reproductive outcome and oxidative stress biomarkers of streptozotocin-induced diabetic rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Rudge,M.V.C.; Damasceno,D.C.; Volpato,G.T.; Almeida,F.C.G.; Calderon,I.M.P.; Lemonica,I.P.
The aim of the present study was to evaluate the effect of Ginkgo biloba treatment (EGb 761, 200 mg kg-1 day-1) administered from day 0 to 20 of pregnancy on maternal reproductive performance and on the maternal and fetal liver antioxidant systems of streptozotocin-induced diabetic Wistar rats. On day 21 of pregnancy, the adult rats (weighing approximately 250 ± 50 g, minimum number = 13/group) were anesthetized to obtain maternal and fetal liver samples for superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and total glutathione (GSH-t) determinations. The uterus was weighed with its contents. The diabetic (G3) and treated diabetic (G4) groups of rats presented significant maternal hyperglycemia, reduced term pregnancy rate,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ginkgo biloba; Diabetes mellitus; Pregnancy; Reproductive outcome; Oxidative stress.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800010

Gastrodin induced HO-1 and Nrf2 up-regulation to alleviate H2O2-induced oxidative stress in mouse liver sinusoidal endothelial cells through p38 MAPK phosphorylation
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhang,Hongbin; Yuan,Bo; Huang,Hanfei; Qu,Siming; Yang,Shikun; Zeng,Zhong.
Nuclear factor erythroid-related factor 2 (Nrf2) has been implicated in several detoxifying and antioxidant defense processes. Nrf2-mediated heme oxygenase-1 (HO-1) expression was demonstrated to play a key role against oxidative stress. Gastrodin (GSTD) is a well-known active compound isolated from the roots of Rhizoma gastrodiae, a plant used in ancient Chinese traditional medicine. The aim of this work was to investigate whether GSTD could alleviate H2O2-induced oxidative stress in mouse liver sinusoidal endothelial cells (LSECs). In LSECs exposed to 1 mM H2O2, treatment with GSTD (1, 10, or 50 µM) resulted in higher cell viability than the untreated control. Treated cells maintained a higher Bcl2/Bax ratio and suppressed caspase-9 expression compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastrodin; Liver sinusoidal endothelial cells; Heme oxygenase 1; Oxidative stress; Hydrogen peroxide.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000611

Short-term levosimendan treatment protects rat testes against oxidative stress
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Yuksel,M.B.; Kavak,S.; Gecit,I.; Basel,H.; Gümrükçüoğlu,H.A.; Demir,H.; Meral,İ.
The objective of this study was to evaluate the effect of short-term levosimendan exposure on oxidant/antioxidant status and trace element levels in the testes of rats under physiological conditions. Twenty male Wistar albino rats were randomly divided into two groups of 10 animals each. Group 1 was not exposed to levosimendan and served as control. Levosimendan (12 µg/kg) diluted in 10 mL 0.9% NaCl was administered intraperitoneally to group 2. Animals of both groups were sacrificed after 3 days and their testes were harvested for the determination of changes in tissue oxidant/antioxidant status and trace element levels. Tissue malondialdehyde (MDA) was significantly lower in the levosimendan group (P &lt; 0.001) than in the untreated control group and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Levosimendan; Oxidative stress; Reactive oxygen species; Rat testes; SOD; GSH-Px; MDA.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800005

Angiotensin-(1-7) relieved renal injury induced by chronic intermittent hypoxia in rats by reducing inflammation, oxidative stress and fibrosis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Lu,W.; Kang,J.; Hu,K.; Tang,S.; Zhou,X.; Yu,S.; Xu,L.
We aimed to study the renal injury and hypertension induced by chronic intermittent hypoxia (CIH) and the protective effects mediated by angiotensin 1-7 [Ang(1-7)]. We randomly assigned 32 male Sprague-Dawley rats (body weight 180-200 g) to normoxia control, CIH, Ang(1-7)-treated normoxia, and Ang(1-7)-treated CIH groups. Systolic blood pressure (SBP) was monitored at the start and end of each week. Renal sympathetic nerve activity (RSNA) was recorded. CTGF and TGF-β were detected by immunohistochemistry and western blotting. Tissue parameters of oxidative stress were also determined. In addition, renal levels of interleukin-6, tumor necrosis factor-α, nitrotyrosine, and hypoxia-inducible factor-1α were determined by immunohistochemistry, immunoblotting,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ang(1-7); Renal injury; Obstructive sleep apnea; Inflammation; Oxidative stress; Fibrosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100604

Inflammation and oxidative stress in heart failure: effects of exercise intensity and duration
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ribeiro-Samora,G.A.; Rabelo,L.A.; Ferreira,A.C.C.; Favero,M.; Guedes,G.S.; Pereira,L.S.M.; Parreira,V.F.; Britto,R.R.
Although acute exercise is apparently pro-inflammatory and increases oxidative stress, it can promote the necessary stress stimulus to train chronic adaptations in patients with chronic heart failure (CHF). This study aimed to compare the effects of exercise intensity and duration on the inflammatory markers soluble tumor necrosis factor receptor (sTNFR1) and interleukin-6 (IL-6), and on oxidative stress [malondialdehyde (MDA) and antioxidant enzymes: catalase (CAT) and superoxide dismutase (SOD)] in individuals with CHF. Eighteen patients performed three exercise sessions: 30 min of moderate-intensity (M30) exercise, 30 min of low-intensity (L30) exercise, and 45 min of low-intensity (L45) exercise. Blood analysis was performed before exercise (baseline),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure; Inflammation; Oxidative stress; Exercise intensity; Exercise duration.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900608

Oxidative stress and cellular immunity in patients with recurrent aphthous ulcers
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Avci,E.; Akarslan,Z.Z.; Erten,H.; Coskun-Cevher,S.
Recurrent aphthous ulcer (RAU) is an inflammatory condition of the oral mucosa characterized by painful, well-circumscribed, single or multiple round or ovoid ulcerations. The exact etiologic factor(s) of these ulcerations are not yet understood. The objective of this study was to evaluate inflammatory processes and free radical metabolism of 25 patients with RAUs compared to 25 healthy controls. The levels of malondialdehyde (MDA) and glutathione (GSH) were determined by high-performance liquid chromatography. Tumor necrosis factor-alpha (TNF-α), interleukin-2 (IL-2), IL-10, and IL-12 were determined by ELISA. Nitric oxide (NO), myeloperoxidase (MPO), total antioxidant status (TAS), and total oxidant status (TOS) levels were measured spectroscopically in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recurrent aphthous ulcer; Oxidative stress; Cytokines.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500355

Hemodialysis improves endothelial venous function in end-stage renal disease
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Silva,A.M.V.; Signori,L.U.; Plentz,R.D.M.; Moreno Jr.,H.; Barros,E.; Belló-Klein,A.; Schaan,B.D.; Irigoyen,M.C.
The objective of the present study was to determine the acute effect of hemodialysis on endothelial venous function and oxidative stress. We studied 9 patients with end-stage renal disease (ESRD), 36.8 ± 3.0 years old, arterial pressure 133.8 ± 6.8/80.0 ± 5.0 mmHg, time on dialysis 55.0 ± 16.6 months, immediately before and after a hemodialysis session, and 10 healthy controls matched for age and gender. Endothelial function was assessed by the dorsal hand vein technique using graded local infusion of acetylcholine (endothelium-dependent venodilation, EDV) and sodium nitroprusside (endothelium-independent venodilation). Oxidative stress was evaluated by measuring protein oxidative damage (carbonyls) and antioxidant defense (total radical trapping...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: End-stage renal disease; Hemodialysis; Oxidative stress; Vascular endothelium.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600008

Genetic responses against nitric oxide toxicity
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Demple,B.
The threat of free radical damage is opposed by coordinated responses that modulate expression of sets of gene products. In mammalian cells, 12 proteins are induced by exposure to nitric oxide (NO) levels that are sub-toxic but exceed the level needed to activate guanylate cyclase. Heme oxygenase 1 (HO-1) synthesis increases substantially, due to a 30- to 70-fold increase in the level of HO-1 mRNA. HO-1 induction is cGMP-independent and occurs mainly through increased mRNA stability, which therefore indicates a new NO-signaling pathway. HO-1 induction contributes to dramatically increased NO resistance and, together with the other inducible functions, constitutes an adaptive resistance pathway that also defends against oxidants such as H2O2. In E. coli, an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; SoxR protein; Heme oxygenase 1; RNA stability; Transcriptional regulation.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100013

Treatment with Ilex paraguariensis (yerba mate) aqueous solution prevents hepatic redox imbalance, elevated triglycerides, and microsteatosis in overweight adult rats that were precociously weaned
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: de Oliveira,E.; Lima,N.S.; Conceição,E.P.S.; Peixoto-Silva,N.; Moura,E.G.; Lisboa,P.C.
Early weaning (EW) leads to overweight, visceral obesity, hyperleptinemia, and insulin resistance in adulthood. Treatment with Ilex paraguariensis (yerba mate) improves obesity and insulin resistance in these animals. Here, we evaluated the effects of chronic treatment with yerba mate on the redox balance and liver morphology of overweight early-weaned rats. To induce EW, we wrapped the dams with bandages to interrupt milk access during the last 3 days of lactation. Control pups (C) had free access to maternal milk for the full 21 days of lactation. On postnatal day (PN) 150, EW offspring were subdivided into the EW+YM group, which received the aqueous extract of yerba mate (1 g/kg bw by gavage once a day for 30 days) and the EW group, which received water...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Early weaning; Programming; Oxidative stress; Steatosis; Liver.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600619

Astragalus polysaccharides improve cardiomyopathy in STZ-induced diabetic mice and heterozygous (SOD2+/-) knockout mice
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ju,J.; Chen,W.; Lai,Y.; Wang,L.; Wang,H.; Chen,W.J.; Zhao,X.; Ye,H.; LI,Y.; Zhang,Y.
Oxidative stress plays an important role in the development of diabetic cardiomyopathy. In the present study, we determined whether the effect of astragalus polysaccharides (APS) on diabetic cardiomyopathy was associated with its impact on oxidative stress. Streptozotocin (STZ)-induced diabetic mice and heterozygous superoxide dismutase (SOD2+/-) knockout mice were administered APS. The hemodynamics, cardiac ultrastructure, and the apoptosis, necrosis and proliferation of cardiomyocytes were assessed to evaluate the effect of APS on diabetic and oxidative cardiomyopathy. Furthermore, H2O2 formation, oxidative stress/damage, and SOD activity in cardiomyocytes were evaluated to determine the effects of APS on cardiac oxidative stress. APS therapy improved...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Cardiomyopathy; Oxidative stress; Superoxide dismutase.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800601

Attenuation of renal ischemic reperfusion injury by salvianolic acid B via suppressing oxidative stress and inflammation through PI3K/Akt signaling pathway
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ma,Z.G.; Xia,H.Q.; Cui,S.L.; Yu,J.
Salvianolic acid B (SAB) is one the major phytocomponents of Radix Salvia miltiorrhiza and exhibit numerous health promoting properties. The objective of the current study was to examine whether SAB exerts a renoprotective effect by attenuating oxidative stress and inflammatory response through activating phosphatidylinositol 3-kinase/serine-threonine kinase B (PI3K/Akt) signaling pathway in a renal ischemic reperfusion rat model. Forty Sprague-Dawley male rats (250–300 g) were obtained and split into four groups with ten rats in each group. The right kidney of all rats was removed (nephrectomy). The rats of the Control group received only saline (occlusion) and served as a sham control group, whereas rats subjected to ischemic reperfusion (IR) insult by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renal ischemic reperfusion; Salvianolic acid B; Oxidative stress; Inflammation; PI3K/Akt pathway.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600607

Lipid peroxidation and total radical-trapping potential of the lungs of rats submitted to chronic and sub-chronic stress
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Torres,R.L.; Torres,I.L.S.; Gamaro,G.D.; Fontella,F.U.; Silveira,P.P.; Moreira,J.S.R.; Lacerda,M.; Amoretti,J.R.; Rech,D.; Dalmaz,C.; Belló,A.A.
Exposure to stress induces a cluster of physiological and behavioral changes in an effort to maintain the homeostasis of the organism. Long-term exposure to stress, however, has detrimental effects on several cell functions such as the impairment of antioxidant defenses leading to oxidative damage. Oxidative stress is a central feature of many diseases. The lungs are particularly susceptible to lesions by free radicals and pulmonary antioxidant defenses are extensively distributed and include both enzymatic and non-enzymatic systems. The aim of the present study was to determine lipid peroxidation and total radical-trapping potential (TRAP) changes in lungs of rats submitted to different models of chronic stress. Adult male Wistar rats weighing 180-230 g...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stress; TBARS; TRAP; Free radicals; Lungs; Oxidative stress.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200004

Effect of dietary fat saturation on lipid metabolism, arachidonic acid turnover and peritoneal macrophage oxidative stress in mice
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Oliveros,L.B.; Videla,A.M.; Giménez,M.S.
We investigated the effects of a saturated fat diet on lipid metabolism and arachidonic acid (AA) turnover in mouse resident peritoneal macrophages. The pro-oxidative effect of this diet was also studied. Female C57BL/6 mice were weaned at 21 days of age and assigned to either the experimental diet containing coconut oil (COCO diet), or the control diet containing soybean oil as fat source (10 mice per group). The fat content of each diet was 15% (w/w). Mice were fed for 6 weeks and then sacrificed. The concentration of total lipids, triglycerides, (LDL + VLDL)-cholesterol, thiobarbituric acid-reactive substances (TBARS) and reduced glutathione were increased in the plasma of mice fed the COCO diet, without changes in phospholipid or total cholesterol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peritoneal macrophage; Coconut oil; Soybean oil; Lipids; Oxidative stress; Arachidonic acid.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300004

Possible in vivo mechanisms involved in photodynamic therapy using tetrapyrrolic macrocycles
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Filip,A.G.; Clichici,S.; Daicoviciu,D.; Ion,R.M.; Tatomir,C.; Rogojan,L.; Opris,I.; Mocan,T.; Olteanu,D.; Muresan,A.
Photodynamic therapy (PDT) mediated by oxidative stress causes direct tumor cell damage as well as microvascular injury. To improve this treatment new photosensitizers are being synthesized and tested. We evaluated the effects of PDT with 5,10,15,20-tetrakis(4-methoxyphenyl)-porphyrin (TMPP) and its zinc complex (ZnTMPP) on tumor levels of malondialdehyde (MDA), reduced glutathione (GSH) and cytokines, and on the activity of caspase-3 and metalloproteases (MMP-2 and -9) and attempted to correlate them with the histological alterations of tumors in 3-month-old male Wistar rats, 180 ± 20 g, bearing Walker 256 carcinosarcoma. Rats were randomly divided into five groups: group 1, ZnTMPP+irradiation (IR) 10 mg/kg body weight; group 2, TMPP+IR 10 mg/kg body...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photodynamic therapy; Oxidative stress; Apoptosis; Tetrapyrrolic macrocycles; Cytokines.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100008

Caryocar brasiliense camb protects against genomic and oxidative damage in urethane-induced lung carcinogenesis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Colombo,N.B.R.; Rangel,M.P.; Martins,V.; Hage,M.; Gelain,D.P.; Barbeiro,D.F.; Grisolia,C.K.; Parra,E.R.; Capelozzi,V.L.
The antioxidant effects of Caryocar brasiliense Camb, commonly known as the pequi fruit, have not been evaluated to determine their protective effects against oxidative damage in lung carcinogenesis. In the present study, we evaluated the role of pequi fruit against urethane-induced DNA damage and oxidative stress in forty 8-12 week old male BALB/C mice. An in vivo comet assay was performed to assess DNA damage in lung tissues and changes in lipid peroxidation and redox cycle antioxidants were monitored for oxidative stress. Prior supplementation with pequi oil or its extract (15 µL, 60 days) significantly reduced urethane-induced oxidative stress. A protective effect against DNA damage was associated with the modulation of lipid peroxidation and low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pequi; Caryocar brasiliense Camb; Oxidative stress; Lung cancer; Urethane; Molecular biology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900852

Melatonin prevents inflammation and oxidative stress caused by abdominopelvic and total body irradiation of rat small intestine
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Guney,Y.; Hicsonmez,A.; Uluoglu,C.; Guney,H.Z.; Ozel Turkcu,U.; Take,G.; Yucel,B.; Caglar,G.; Bilgihan,A.; Erdogan,D.; Nalca Andrieu,M.; Kurtman,C.; Zengil,H.
We investigated the day-night differences in intestinal oxidative-injury and the inflammatory response following total body (TB) or abdominopelvic (AP) irradiation, and the influence of melatonin administration on tissue injury induced by radiation. Rats (male Wistar, weighing 220-280 g) in the irradiated groups were exposed to a dose of 8 Gy to the TB or AP region in the morning (resting period - 1 h after light onset) or evening (activity span - 13 h after light onset). Vehicle or melatonin was administered immediately before, immediately after and 24 h after irradiation (10, 2.0 and 10 mg/kg, ip, respectively) to the irradiated rats. AP (P &lt; 0.05) and TB (P &lt; 0.05) irradiation applied in the morning caused a significant increase in thiobarbituric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Irradiation; Melatonin; Circadian rhythm; Oxidative stress; Thiobarbituric acid reactive substances; Myeloperoxidase.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000002

The superoxide anion donor, potassium superoxide, induces pain and inflammation in mice through production of reactive oxygen species and cyclooxygenase-2
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Maioli,N.A.; Zarpelon,A.C.; Mizokami,S.S.; Calixto-Campos,C.; Guazelli,C.F.S.; Hohmann,M.S.N.; Pinho-Ribeiro,F.A.; Carvalho,T.T.; Manchope,M.F.; Ferraz,C.R.; Casagrande,R.; Verri Jr,W.A.
It is currently accepted that superoxide anion (O2•−) is an important mediator in pain and inflammation. The role of superoxide anion in pain and inflammation has been mainly determined indirectly by modulating its production and inactivation. Direct evidence using potassium superoxide (KO2), a superoxide anion donor, demonstrated that it induced thermal hyperalgesia, as assessed by the Hargreaves method. However, it remains to be determined whether KO2 is capable of inducing other inflammatory and nociceptive responses attributed to superoxide anion. Therefore, in the present study, we investigated the nociceptive and inflammatory effects of KO2. The KO2-induced inflammatory responses evaluated in mice were: mechanical hyperalgesia (electronic version of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Superoxide anion; Potassium superoxide; Pain; Inflammation; Oxidative stress.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400321

Antioxidant responses of Laeonereis acuta (Polychaeta) after exposure to hydrogen peroxide
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Rosa,C.E. da; Bianchini,A.; Monserrat,J.M.
The effects of H2O2 were evaluated in the estuarine worm Laeonereis acuta (Polychaeta, Nereididae) collected at the Patos Lagoon estuary (Southern Brazil) and maintained in the laboratory under controlled salinity (10 psu diluted seawater) and temperature (20°C). The worms were exposed to H2O2 (10 and 50 µM) for 4, 7, and 10 days and the following variables were determined: oxygen consumption, catalase (CAT) and glutathione peroxidase activity in both the supernatant and pellet fractions of whole body homogenates. The concentrations of non-protein sulfhydryl and lipid peroxides (LPO) were also measured. The oxygen consumption response was biphasic, decreasing after 4 days and increasing after 7 and 10 days of exposure to 50 µM H2O2 (P &lt; 0.05). At the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Worms; Laeonereis acuta; Hydrogen peroxide; Oxidative stress; Antioxidant enzymes; Oxygen consumption.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200007

Pleiotropic effects of simvastatin in physically trained ovariectomized rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Bernardes,N.; Brito,J.O.; Fernandes,T.G.; Llesuy,S.F.; Irigoyen,M.C.; Belló-Klein,A.; De Angelis,K.
This study tested the hypothesis that simvastatin treatment can improve cardiovascular and autonomic functions and membrane lipoperoxidation, with an increased effect when applied to physically trained ovariectomized rats. Ovariectomized rats were divided into sedentary, sedentary+simvastatin and trained+simvastatin groups (n = 8 each). Exercise training was performed on a treadmill for 8 weeks and simvastatin (5 mg/kg) was administered in the last 2 weeks. Blood pressure (BP) was recorded in conscious animals. Baroreflex sensitivity was evaluated by the tachycardic and bradycardic responses to BP changes. Cardiac vagal and sympathetic effects were determined using methylatropine and propranolol. Oxidative stress was evaluated based on heart and liver...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Menopause; Exercise training; Simvastatin; Autonomic function; Oxidative stress.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000500447

Selegiline increases heme oxygenase-1 expression and the cytotoxicity produced by dopamine treatment of neuroblastoma SK-N-SH cells
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Rieder,C.R.M.; Williams,A.C.; Ramsden,D.B.
Increased dopamine catabolism may be associated with oxidative stress and neuronal cell death in Parkinson's disease. The present study was carried out to examine the effect of dopamine on the expression of heme oxygenase-1 and -2 (HO-1 and HO-2) in human neuroblastomas (SK-N-SH cell line) and the effects of selegiline and antioxidants on this expression. Cells were kept with close control of pH and were incubated with varying concentrations of dopamine (0.1-100 µM) for 24 h. HO-1 and HO-2 cDNA probes were prepared by reverse transcription-polymerase chain reaction amplification. The mRNA expression of HO-1 and HO-2 was measured by Northern blot analysis. The levels of HO-1 mRNA increased after dopamine treatment, in a dose-dependent manner, in all cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Parkinson's disease; Dopamine; Selegiline.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700015

Peroxiredoxin isoforms are associated with cardiovascular risk factors in type 2 diabetes mellitus
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: El Eter,E.; Al-Masri,A.A.
The production of oxygen free radicals in type 2 diabetes mellitus contributes to the development of complications, especially the cardiovascular-related ones. Peroxiredoxins (PRDXs) are antioxidant enzymes that combat oxidative stress. The aim of this study was to investigate the associations between the levels of PRDX isoforms (1, 2, 4, and 6) and cardiovascular risk factors in type 2 diabetes mellitus. Fifty-three patients with type 2 diabetes mellitus (28F/25M) and 25 healthy control subjects (7F/18M) were enrolled. We measured the plasma levels of each PRDX isoform and analyzed their correlations with cardiovascular risk factors. The plasma PRDX1, -2, -4, and -6 levels were higher in the diabetic patients than in the healthy control subjects. PRDX2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peroxiredoxins; Type 2 diabetes mellitus; Oxidative stress; Cardiovascular risk factors.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500465

Liver oxidative stress of the grey mullet Mugil cephalus presents seasonal variations in Ennore estuary
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Padmini,E.; Vijaya Geetha,B.; Usha Rani,M.
The objective of this study was to determine the liver oxidative stress status of grey mullets living in heavy-metal-rich polluted Ennore estuary compared with unpolluted Kovalam estuary. Fish were collected from both estuaries during the monsoon and summer seasons from October 2004 to September 2006. Fish liver homogenate (N = 20 per group) was prepared for evaluating oxidative stress parameters. Fish living in the polluted estuary had significantly higher lipid oxidation products, conjugated dienes (0.346 ± 0.017 vs 0.141 ± 0.012 DA233/mg protein), lipid hydroperoxides (0.752 ± 0.032 vs 0.443 ± 0.03 nmol/mg protein), and lipid peroxides (3.447 ± 0.14vs 1.456 ± 0.096 nmol MDA/mg protein) than those of the unpolluted estuary during the summer. In contrast,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Antioxidant defense; Estuary; Grey mullets; Oxidative stress; Pollutants.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100002

Swimming training prevents coronary endothelial dysfunction in ovariectomized spontaneously hypertensive rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Claudio,E.R.G.; Almeida,S.A.; Mengal,V.; Brasil,G.A.; Santuzzi,C.H.; Tiradentes,R.V.; Gouvea,S.A.; Bissoli,N.S.; Santos,R.L.; Abreu,G.R.
Estrogen deficiency and hypertension are considered major risk factors for the development of coronary heart disease. On the other hand, exercise training is considered an effective form to prevent and treat cardiovascular diseases. However, the effects of swimming training (SW) on coronary vascular reactivity in female ovariectomized hypertensive rats are not known. We aimed to evaluate the effects of SW on endothelium-dependent coronary vasodilation in ovariectomized hypertensive rats. Three-month old spontaneously hypertensive rats (SHR, n=50) were divided into four groups: sham (SH), sham plus swimming training (SSW), ovariectomized (OVX), and ovariectomized plus swimming training (OSW). The SW protocol (5 times/week, 60 min/day) was conducted for 8...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swimming training; Ovariectomy; Coronary reactivity; Oxidative stress; Antioxidant enzymes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100607

Protective mechanism of agmatine pretreatment on RGC-5 cells injured by oxidative stress
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Iizuka,Y.; Hong,S.; Kim,C.Y.; Yang,W.I.; Lee,J.E.; Seong,G.J.
Agmatine has neuroprotective effects on retinal ganglion cells (RGCs) as well as cortical and spinal neurons. It protects RGCs from oxidative stress even when it is not present at the time of injury. As agmatine has high affinity for various cellular receptors, we assessed protective mechanisms of agmatine using transformed RGCs (RGC-5 cell line). Differentiated RGC-5 cells were pretreated with 100 μM agmatine and consecutively exposed to 1.0 mM hydrogen peroxide (H2O2). Cell viability was determined by measuring lactate dehydrogenase (LDH), and the effects of selective alpha 2-adrenergic receptor antagonist yohimbine (0-500 nM) and N-methyl-D-aspartic acid (NMDA) receptor agonist NMDA (0-100 µM) were evaluated. Agmatine’s protective effect was compared to...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Agmatine; Neuroprotection; Oxidative stress; Retinal ganglion cell.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400006

Serum total bilirubin levels are negatively correlated with metabolic syndrome in aged Chinese women: a community-based study
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhong,P.; Sun,D.M.; Wu,D.H.; Li,T.M.; Liu,X.Y.; Liu,H.Y.
We evaluated serum total bilirubin levels as a predictor for metabolic syndrome (MetS) and investigated the relationship between serum total bilirubin levels and MetS prevalence. This cross-sectional study included 1728 participants over 65 years of age from Eastern China. Anthropometric data, lifestyle information, and previous medical history were collected. We then measured serum levels of fasting blood-glucose, total cholesterol, triglycerides, and total bilirubin, as well as alanine aminotransferase activity. The prevalence of MetS and each of its individual component were calculated per quartile of total bilirubin level. Logistic regression was used to assess the correlation between serum total bilirubin levels and MetS. Total bilirubin level in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Total bilirubin; Metabolic syndrome; Oxidative stress.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200701

Protective effect of N-acetylcysteine against oxygen radical-mediated coronary artery injury
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Rodrigues,A.J.; Évora,P.R.B.; Schaff,H.V.
The present study investigated the protective effect of N-acetylcysteine (NAC) against oxygen radical-mediated coronary artery injury. Vascular contraction and relaxation were determined in canine coronary arteries immersed in Kreb's solution (95% O2-5% CO2), incubated or not with NAC (10 mM), and exposed to free radicals (FR) generated by xanthine oxidase (100 mU/ml) plus xanthine (0.1 mM). Rings not exposed to FR or NAC were used as controls. The arteries were contracted with 2.5 µM prostaglandin F2alpha. Subsequently, concentration-response curves for acetylcholine, calcium ionophore and sodium fluoride were obtained in the presence of 20 µM indomethacin. Concentration-response curves for bradykinin, calcium ionophore, sodium nitroprusside, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coronary arteries; N-acetylcysteine; Endothelium; Oxidative stress; Nitric oxide.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800012

Influence of eNOS gene polymorphism on cardiometabolic parameters in response to physical training in postmenopausal women
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Esposti,R.D.; Sponton,C.H.G.; Malagrino,P.A.; Carvalho,F.C.; Peres,E.; Puga,G.M.; Novais,I.P.; Albuquerque,D.M.; Rodovalho,C.; Bacci,M.; Zanesco,A.
The health-promoting effects of exercise training (ET) are related to nitric oxide (NO) production and/or its bioavailability. The objective of this study was to determine whether single nucleotide polymorphism of the endothelial NO synthase (eNOS) gene at positions -786T&gt;C, G894T (Glu298Asp) and at the variable number of tandem repeat (VNTR) Intron 4b/a would interfere with the cardiometabolic responses of postmenopausal women submitted to physical training. Forty-nine postmenopausal women were trained in sessions of 30-40 min, 3 days a week for 8 weeks. Genotypes, oxidative stress status and cardiometabolic parameters were then evaluated in a double-blind design. Both systolic and diastolic blood pressure values were significantly reduced after ET,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise training; ENOS polymorphism; Oxidative stress; Nitric oxide; Women.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900005

Effect of unsaturated fatty acids on myocardial performance, metabolism and morphology
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Pinotti,M.F.; Silva,M.D.P.; Sugizaki,M.M.; Diniz,Y.S.; Sant'Ana,L.S.; Aragon,F.F.; Padovani,C.R.; Novelli,E.L.B.; Cicogna,A.C.
Diets rich in saturated fatty acids are one of the most important causes of atherosclerosis in men, and have been replaced with diets rich in unsaturated fatty acids (UFA) for the prevention of this disorder. However, the effect of UFA on myocardial performance, metabolism and morphology has not been completely characterized. The objective of the present investigation was to evaluate the effects of a UFA-rich diet on cardiac muscle function, oxidative stress, and morphology. Sixty-day-old male Wistar rats were fed a control (N = 8) or a UFA-rich diet (N = 8) for 60 days. Myocardial performance was studied in isolated papillary muscle by isometric and isotonic contractions under basal conditions after calcium chloride (5.2 mM) and ß-adrenergic stimulation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fatty acids; Cardiac function; Oxidative stress; Morphology; Catalase; Superoxide dismutase.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200019

Paradoxical effect of a pequi oil-rich diet on the development of atherosclerosis: balance between antioxidant and hyperlipidemic properties
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Aguilar,E.C.; Jascolka,T.L.; Teixeira,L.G.; Lages,P.C.; Ribeiro,A.C.C.; Vieira,E.L.M.; Peluzio,M.C.G.; Alvarez-Leite,J.I.
Pequi is the fruit of Caryocar brasiliense and its oil has a high concentration of monounsaturated and saturated fatty acids, which are anti- and pro-atherogenic agents, respectively, and of carotenoids, which give it antioxidant properties. Our objective was to study the effect of the intake of a cholesterol-rich diet supplemented with pequi oil, compared to the same diet containing soybean oil, on atherosclerosis development, and oxidative stress in atherosclerosis-susceptible LDL receptor-deficient mice (LDLr-/-, C57BL/6-background). Female mice were fed a cholesterol-rich diet containing 7% soybean oil (Soybean group, N = 12) or 7% pequi oil (Pequi group, N = 12) for 6 weeks. The Pequi group presented a more atherogenic lipid profile and more advanced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pequi oil; Fatty acid; Lipids; Atherosclerosis; Oxidative stress; Cholesterol.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700006

Liver mitochondrial dysfunction and oxidative stress in the pathogenesis of experimental nonalcoholic fatty liver disease
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Oliveira,C.P.M.S.; Coelho,A.M.M.; Barbeiro,H.V.; Lima,V.M.R.; Soriano,F.; Ribeiro,C.; Molan,N.A.T.; Alves,V.A.F.; Souza,H.P.; Machado,M.C.C.; Carrilho,F.J.
Oxidative stress and hepatic mitochondria play a role in the pathogenesis of nonalcoholic fatty liver disease. The aim of the present study was to evaluate the role of hepatic mitochondrial dysfunction and oxidative stress in the pathogenesis of the disease. Fatty liver was induced in Wistar rats with a choline-deficient diet (CD; N = 7) or a high-fat diet enriched with PUFAs-omega-3 (H; N = 7) for 4 weeks. The control group (N = 7) was fed a standard diet. Liver mitochondrial oxidation and phosphorylation were measured polarographically and oxidative stress was estimated on the basis of malondialdehyde and glutathione concentrations. Moderate macrovacuolar liver steatosis was observed in the CD group and mild liver steatosis was observed in the periportal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatic mitochondrial dysfunction; Oxidative stress; Nonalcoholic fatty liver disease; Choline-deficient diet; High-fat diet.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200004

Protective effects of organoselenium compounds against methylmercury-induced oxidative stress in mouse brain mitochondrial-enriched fractions
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Meinerz,D.F.; de Paula,M.T.; Comparsi,B.; Silva,M.U.; Schmitz,A.E.; Braga,H.C.; Taube,P.S.; Braga,A.L.; Rocha,J.B.T.; Dafre,A.L.; Farina,M.; Franco,J.L.; Posser,T.
We evaluated the potential neuroprotective effect of 1-100 µM of four organoselenium compounds: diphenyl diselenide, 3’3-ditri-fluoromethyldiphenyl diselenide, p-methoxy-diphenyl diselenide, and p-chloro-diphenyl diselenide, against methylmercury-induced mitochondrial dysfunction and oxidative stress in mitochondrial-enriched fractions from adult Swiss mouse brain. Methylmercury (10-100 µM) significantly decreased mitochondrial activity, assessed by MTT reduction assay, in a dose-dependent manner, which occurred in parallel with increased glutathione oxidation, hydroperoxide formation (xylenol orange assay) and lipid peroxidation end-products (thiobarbituric acid reactive substances, TBARS). The co-incubation with diphenyl diselenide (100 µM) completely...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methylmercury; Mitochondria; Oxidative stress; Organoselenium compounds; Diphenyl diselenide.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100012

Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris
Provedor de dados: Brazilian Journal of Microbiology
Autores: Zepeda,Andrea B.; Figueroa,Carolina A.; Abdalla,Dulcineia S.P.; Maranhão,Andrea Q.; Ulloa,Patricio H.; Pessoa Jr,Adalberto; Farías,Jorge G.
Pichia pastoris is methylotrophic yeast used as an efficient expression system for heterologous protein production. In order to evaluate the effects of temperature (10 and 30 °C) and methanol (1 and 3% (v/v)) on genetically-modified Pichia pastoris, different biomarkers were evaluated: Heat stress (HSF-1 and Hsp70), oxidative stress (OGG1 and TBARS) and antioxidant (GLR). Three yeast cultures were performed: 3X = 3% methanol-10 °C, 4X = 3% methanol-30 °C, and 5X = 1% methanol-10°C. The expression level of HIF-1α, HSF-1, HSP-70 and HSP-90 biomarkers were measured by Western blot and in situ detection was performed by immunocytochemistry. Ours results show that at 3% methanol -30 °C there is an increase of mitochondrial OGG1 (mtOGG1), Glutathione Reductase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Recombinant yeast; Heat stress protein; DNA damage.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200014

Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress
Provedor de dados: Brazilian Journal of Microbiology
Autores: Sá,Rafael A. de; Castro,Frederico A.V. de; Eleutherio,Elis C.A.; Souza,Raquel M. de; Silva,Joaquim F.M. da; Pereira,Marcos D.
Propolis is a natural product widely used for humans. Due to its complex composition, a number of applications (antimicrobial, antiinflammatory, anesthetic, cytostatic and antioxidant) have been attributed to this substance. Using Saccharomyces cerevisiae as a eukaryotic model we investigated the mechanisms underlying the antioxidant effect of propolis from Guarapari against oxidative stress. Submitting a wild type (BY4741) and antioxidant deficient strains (ctt1∆, sod1∆, gsh1∆, gtt1∆ and gtt2∆) either to 15 mM menadione or to 2 mM hydrogen peroxide during 60 min, we observed that all strains, except the mutant sod1∆, acquired tolerance when previously treated with 25 µg/mL of alcoholic propolis extract. Such a treatment reduced the levels of ROS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propolis; Antioxidant; Oxidative stress; Saccharomyces cerevisiae.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300051

Ginkgo biloba extract (EGb 761) attenuates oxidative stress induction in erythrocytes of sickle cell disease patients
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Furman,Aline Emmer Ferreira; Henneberg,Railson; Hermann,Priscila Bacarin; Leonart,Maria Suely Soares; Nascimento,Aguinaldo José do.
Sickle cell disease promotes hemolytic anemia and occlusion of small blood vessels due to the presence of high concentrations of hemoglobin S, resulting in increased production of reactive oxygen species and decreased antioxidant defense capacity. The aim of this study was to evaluate the protective action of a standardized extract of Ginkgo biloba (EGb 761), selected due to its high content of flavonoids and terpenoids, in erythrocytes of patients with sickle cell anemia (HbSS, SS erythrocytes) subjected to oxidative stress using tert-butylhydroperoxide or 2,2-azobis-(amidinepropane)-dihydrochloride, in vitro. Hemolysis indexes, reduced glutathione, methemoglobin concentrations, lipid peroxidation, and intracellular reactive oxygen species were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ginkgo biloba; Oxidative stress; Erythrocytes; Azo compounds; Sickle cell anemia.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400009

Modulation of the oxidative stress in malaria infection by clotrimazole
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Rodrigues Henriques,Juan Ricardo; Gamboa de Domínguez,Neira.
Antimycotic clotrimazole (CTZ) has demonstrated remarkable activity against Plasmodium falciparum in vitro and in vivo. Hemoglobin degradation by Plasmodium parasites makes amino acids available for protein synthesis, inducing oxidative stress in infected cells and producing free heme. These events represent biochemical targets for potential antimalarials. In this study, we have tested the ability of CTZ to modify the oxidative status in Plasmodium berghei-infected erythrocytes. After hemolysis, activities of superoxide dismutase (SOD), catalase (CAT), glutathione cycle and NADPH+H+-producing dehydrogenases were investigated using UV-visible spectrophotometry. Thiobarbituric acid reactive substances (TBARS) were evaluated as a marker of lipid damage....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-Hematin; Clotrimazole/modulatory ability; Clotrimazole/oxidative stress; Oxidative stress; Plasmodium berghei; Eritrócitos infectados.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300019

Protective effects of Urtica dioica L. seed extract on liver tissue injury and antioxidant capacity in irradiated rats
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Yıldızhan,Kenan; Demirtaş,Ömer Can; Uyar,Ahmet; Huyut,Zübeyir; Çakir,Tahir; Keleş,Ömer Faruk; Yener,Zabit.
Radiotherapy is often used for the treatment of cancer. However, it causes some side effects in patients. This study aimed to determine the hepatoprotective effects of Urtica dioica L. seed-extract (UDSE) in radiation-induced liver injury. Thirty-two male rats were randomly divided into 4 groups (n=8): control(C) group: no action was taken; radiation (R) group: irradiation was administrated at 5Gy single-fraction, radiation with UDSE(R+UDSE) group: irradiation was administrated at 5 Gy single-fraction and animals were fed pellets with 30 mL UDSE/kg; UDSE group: animals were fed pellets with 30 mL UDSE/kg. All of the experiments were performed in all of the groups over 10 days. Malondialdehyde (MDA) and reduced-glutathione (GSH) levels and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiation; Urtica dioica L; Seed extract; Oxidative stress; Antioxidant; Tissue injury.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100536

Relationship between oxidative stress and diabetic osteopenia in premenopausal rats
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Duarte,Valéria Morgiana Gualberto; Rodrigues,Alanna de Sousa; Rezende,Luciana Augusto de; Ramos,Ana Maria de Oliveira; Souza,Rodrigo Medeiros de; Freire Neto,Francisco Paulo; Medeiros,Aldo da Cunha; Brandão Neto,José; Almeida,Maria das Graças; Rezende,Adriana Augusto de.
The relationship between lipid peroxidation, antioxidant defense and diabetic osteopenia remains unclear. This study evaluated the relationship among lipid peroxidation index, antioxidant defense parameters and bone metabolism in a premenopausal diabetic model using measures including thiobarbituric acid-reactive substances concentration (TBARS) and reduced glutathione (GSH) content in brain homogenates, histomorphometric analysis, biomechanical testing and bone mineral density (BMD). Female Wistar rats with regular estrous cycle were divided into two groups: Group 1: control rats (n = 15) and Group 2: diabetic rats (n = 15). Diabetes was induced by alloxan and confirmed by glycemia &amp;gt;250 mg/dL. The lipid peroxidation index, measured by TBARS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Lipid peroxidation; Diabetes mellitus; Diabetic osteopenia/experimental study.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300018

Assessment of anti-diabetic activity of a novel hydrazine-thiazole derivative: in vitro and in vivo method
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Resende,Marina Ferrara de; Lino,Cleudiomar Inácio; Souza-Fagundes,Elaine Maria de; Rettore,João Vitor Paes; Oliveira,Renata Barbosa de; Labanca,Renata Adriana.
Diabetes mellitus is a chronic disease resulting in oxidative stress that promotes tissue damage. The appearance of this disease is highly related to lifestyle and food of the population, being of great interest to search for a dietary supplement that can also act by reducing oxidative alterations. Based on the broad range of biological activity of thiazole derivatives, this work aimed to evaluate the in vitro antioxidant activity of a novel hydrazine-thiazole derivative and studies in vivo. In in vivo experiments, the liver extracts of healthy and diabetic Wistar rats were used, with analysis to determine the enzymatic activity of SOD, CAT, GPx, and GR, and determination of lipid peroxidation. Finally, in the blood of these animals, biochemical parameters...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Diabetes mellitus; Curcumin; Thiazole derivatives; Dietary supplement.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100509

Comparison of the antioxidant potential of antiparkinsonian drugs in different in vitro models
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Farias,Carine Coneglian de; Bonifácio,Kamila Landucci; Matsumoto,Andressa Keiko; Higachi,Luciana; Casagrande,Rúbia; Moreira,Estefânia Gastaldello; Barbosa,Décio Sabbatini.
Parkinson's disease (PD) is characterized by progressive degeneration of dopaminergic neurons in the substantia nigra pars compacta. Furthermore, oxidative stress plays a role in PD, causing or contributing to the neurodegenerative process. Currently PD has only symptomatic treatment and still nothing can be done to stop the degenerative process of the disease. This study aimed to comparatively evaluate the antioxidant capacity of pramipexole, selegeline and amantadine in different in vitrostudies and to offer possible explanations on the molecular antioxidant mechanisms of these drugs. In vitro, the antioxidant capacity of the drugs was assessed by the ability of antiparkinsonian drugs to decrease or scavenge ROS in the neutrophil respiratory burst,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parkinson´s disease; Oxidative stress; Antiparkinsonian drugs/in vitrostudies; Antioxidants.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400819

Effects of Cinnamomum cassia extract on oxidative stress, immunreactivity of iNOS and impaired thoracic aortic reactivity induced by type II diabetes in rats
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Uslu,Gözde Atila; Gelen,Volkan; Uslu,Hamit; Özen,Hasan.
Type II diabetes is known to cause neuropathy, nephropathy and retinopathy. However, cardiovascular disorders associated with diabetes have been ignored. In traditional medicine, cinnamon (Cinnamomum cassia) bark has been used for its abilities to relieve fever, inflammation and chronic bronchitis. In the present study, the effect of Cinnamomum cassia extract (CN) on the thoracic aorta in an experimental type II diabetes model was investigated. In rats administered with nicotinamide + streptozotocin, significant endothelial dysfunction and oxidative stress were characterised by increased inducible nitric oxide synthase (iNOS) and decreased insulin/proinsulin levels. This impairment was prevented by administering 1000 mg/kg metformin or 500-1000-1500 mg/kg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type II diabetes; Oxidative stress; INOS; Thoracic aorta reactivity; Cinnamomum cassia/effects.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300628

Coenzyme Q10 and its effects in the treatment of neurodegenerative diseases
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Santos,Graciela Cristina dos; Antunes,Lusânia Maria Greggi; Santos,Antonio Cardozo dos; Bianchi,Maria de Lourdes Pires.
According to clinical and pre-clinical studies, oxidative stress and its consequences may be the cause or, at least, a contributing factor, to a large number of neurodegenerative diseases. These diseases include common and debilitating disorders, characterized by progressive and irreversible loss of neurons in specific regions of the brain. The most common neurodegenerative diseases are Parkinson's disease, Huntington's disease, Alzheimer's disease and amyotrophic lateral sclerosis. Coenzyme Q10 (CoQ10) has been extensively studied since its discovery in 1957. It is a component of the electron transportation chain and participates in aerobic cellular respiration, generating energy in the form of adenosine triphosphate (ATP). The property of CoQ10 to act as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coenzyme Q10; Antioxidant; Oxidative stress; Neurodegenerative diseases.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400002

Effect of metformin on semen quality
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Banihani,Saleem Ali.
ABSTRACT Various studies have linked metformin, a universally antidiabetic drug, with semen quality; however, such a direct link has not been established. This review systematically addresses and summarizes the effect of metformin on semen quality, particularly sperm function. We searched the MEDLINE electronic database for English articles and abstracts containing the key words 'metformin' and 'sperm', and relevant articles were reviewed. In summary, metformin appears to have improved and provided positive impact on sperm quality. This effect may be due to the ability of metformin to reduce oxidative stress and lipid peroxidation, enhance 5'-AMP activated protein kinase activity, and restore the normal levels of pituitary-gonadal hormones. However,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metformin/effects; Metformin/review; Metformin/semen quality; Sperm/study; Testosterone; Oxidative stress; 5'-AMP activated protein kinase.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400591

Passiflora incarnata treatment during gestation and lactation: toxicological and antioxidant evaluation in wistar dams
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Boll,Karine Maria; Bortolasci,Chiara Cristina; Zaminelli,Tiago; Veríssimo,Luiz Fernando; Bacchi,André Demambre; Higachi,Luciana; Barbosa,Décio Sabbatini; Moreira,Estefânia Gastaldello.
Passiflora incarnata is marketed in many countries as a phytomedicine. Even though the directions of most marketed phytomedicines recommend them to be used under medical supervision, reproductive and developmental studies are sparse and not mandatory for regulatory purposes. In this study, a reproductive toxicity evaluation of P. incarnata was conducted in Wistar rats gavaged (30 or 300 mg/kg) during pregnancy and lactation. Moreover, considering that antioxidant properties have been attributed to flavonoids present in the genus Passiflora, it was also evaluated the antioxidant/pro-oxidant balance in the plasma of these dams and the antioxidant potential in an in vitro test. P. incarnata treatment did not influence dams´ body weight as well as reproductive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phytomedicines/evaluation; Passiflora incarnata/use in pregnancy; Passiflora incarnata/use in lactation; Passiflora incarnata/reproductive toxicity/experimental study; Pregnancy/use of phytomedicines; Lactation/use of phytomedicines; Oxidative stress.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200353

Beta-glucan attenuates cerebral ischemia/reperfusion-induced neuronal injury in a C57BL/J6 mouse model
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Kaya,Kürşat; Çiftçi,Osman; Öztanır,Mustafa Namık; Taşlıdere,Elif; Türkmen,Neşe Başak.
Beta-glucans (βg), that have many useful effects on human health, are natural polysaccharides. Our aim in this study was to determine useful effect of βg against oxidative and neuronal damage caused by global cerebral ischemia/reperfusion (IR) in stroke imitated mice via surgical operation. A total of 40 mice divided into four equal groups randomly. The group 1 (sham operated) was kept as control. Bilateral carotid arteries of subjects in group 2 (I/R) and group 4 (I/ R + βg) were clipped for 15 min, and the mice in group 4 (I/R + βg) were treated with βg (50 mg/kg/day), while the mice in group 2 (I/R) were treated with only vehicle for 10 days. The mice of group 3 (βg) were treated with βg for 10 days without carotid occlusion. Global cerebral I/R...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Global cerebral I/R; Oxidative stress; Neuronal damage; Beta-glucan; C57BL/J6 mice.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100570

Protective effect of luteolin on the transgenic Drosophila model of Parkinson’s disease
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Siddique,Yasir Hasan; Jyoti,Smita; Naz,Falaq.
In the present study we have studied the effect of 25, 50, 75 and 100 µM of luteolin on the transgenic Drosophila expressing human alpha synuclein. The doses of luteolin were established in diet and the PD flies were allowed to feed on it for 24 days. After 24 days of exposure the flies were assayed for climbing assay, oxidative stress markers, caspase-3 &amp; 9 activity and dopamine content. The immunohistochemistry was also performed on the brain sections for the activity of tyrosine hydroxylase. The exposure of luteolin showed a dose dependent delay in the loss of climbing ability and activity, reduction in oxidative stress markers, caspase-3&amp;9 activities and results in an increase in the dopamine content. The results obtained for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Luteolin; Parkinson’s disease; Drosophila; Oxidative stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300627

Activity of alternative oxidase and plant uncoupling mitochondrial protein in potato tubers stored at low temperature or submitted to artificial aging
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Pinheiro,Hugo Alves; Borges,Regis; Silva,Marco Aurélio Pedron e; Centeno,Danilo Cruz.
Potato tubers (Solanum tuberosum L. cv. Binje) were stored at 18 and 4ºC in order to verify changes in mitochondrial metabolism, especially in the activities of the alternative oxidase (AOX) and plant uncoupling mitochondria protein (PUMP). Tubers were also sliced and submitted to artificial aging. The oxygen consumption rate (OCR), respiratory control (RC) and ADP/O ratio for mitochondria obtained after storage at 18ºC were not altered, regardless of the time of storage. Considerable increment in OCR, for both respiratory states 3 and 4, was observed after storing the tubers for five and ten days at 4ºC; and it was accomplished by reductions in RC and ADP/O. The AOX activity was evident after five days at 4ºC, and it was enhanced after ten days of storage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum tuberosum L.; Mitochondrial swelling; Oxidative stress; Respiration.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202004000200001

Changes in nitrate reductase activity and oxidative stress response in the moss Polytrichum commune subjected to chromium, copper and zinc phytotoxicity
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Panda,Sanjib Kumar; Choudhury,Shuvasish.
The main aim of this paper was to investigate the effect of chromium (Cr), copper (Cu) and zinc (Zn) on nitrate reductase (NR) activity and oxidative stress responses in the moss Polytrichum commune. Cr, Cu and Zn resulted in the inhibition of NR activity. A decline in total chlorophyll content was observed after 24 and 48 h of metal treatment. Accumulation of the metals showed a dose and time dependent increase. High accumulation of Cu, Cr and Zn were seen in moss shoots after 24 and 48 h of treatment. Treatment of Cr, Cu and Zn for 24 or 48 h resulted in the increase of malondialdehyde (MDA) content in moss shoots. The highest increase was observed in shoots under Cu treatment followed by Cr and Zn. The MDA content was significantly higher after 48h....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Chromium; Copper; Malondialdehyde; Nitrate reductase; Oxidative stress; Polytrichum commune; Zinc.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000200001

Chromium stress in plants
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Panda,S.K.; Choudhury,S.
The article presents an overview of the mechanism of chromium stress in plants. Chromium is known to be a toxic metal that can cause severe damage to plants and animals. Chromium-induced oxidative stress involves induction of lipid peroxidation in plants that causes severe damage to cell membranes. Oxidative stress induced by chromium initiates the degradation of photosynthetic pigments causing decline in growth. High chromium concentration can disturb the chloroplast ultrastructure thereby disturbing the photosynthetic process. Like copper and iron, chromium is also a redox metal and its redox behaviour exceeds that of other metals like Co, Fe, Zn, Ni, etc. The redox behaviour can thus be attributed to the direct involvement of chromium in inducing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Chromium; Fenton reactions; Metallothioneins; Oxidative stress.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000100008

Cadmium-induced oxidative stress and antioxidative enzyme response in water hyacinth and salvinia
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Vestena,Silvane; Cambraia,Jose; Ribeiro,Cleberson; Oliveira,Juraci A.; Oliva,Marco A.
The reactive oxygen species generation, lipid peroxidation and antioxidative enzyme response of water hyacinth and salvinia to Cd were evaluated. Cadmium was absorbed/accumulated mainly in the roots, but significant amounts also translocated to the leaves. No Cd effect on dry weight was detected, although toxicity symptoms were visible. Superoxide and H2O2 concentrations increased, in addition to lipid peroxidation in both species, especially in the leaves of salvinia. In general, antioxidative enzyme activities were reduced in both species following Cd treatment, especially in salvinia. Glutathione peroxidase (GPX, EC 1.11.1.9) activity decreased in water hyacinth but increased in salvinia. Glutathione S-transferase (GST, EC 2.5.1.18) activity increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquatic plants; Eichhornia crassipes; Metal toxicity; Oxidative stress; Salvinia auriculata.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000200005

Epibrassinolide regulated synthesis of polyamines and auxins in Raphanus sativus L. seedlings under Cu metal stress
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Choudhary,Sikander P.; Bhardwaj,Renu; Gupta,Bishan D.; Dutt,Prabhu; Kanwar,Mukesh; Arora,Priya.
The effects of various conc of (10-11, 10-9 and 10-7 M) 24-epibrassinolide (24-epiBL) on polyamines and auxins contents was analyzed in 7days old seedlings of Raphanus sativus L. cv. Pusa chetki under copper metal stress. Cu metal (0.2mM) treatment significantly increased endogenous levels of putrescine, spermidine and spermine when compared with control values. Cadaverine content got decreased under metal stress. Endogenous polyamines showed varying levels in seedlings when applying 24-epiBL treatment only. Maximum increase was found in putrescine, cadaverine and spermidine in 10-11, 10-9 and 10-7 M conc of 24-epiBL respectively when applied alone to R. sativus seedlings. However, metal treatment supplemented with 24-epiBL revealed small increase in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Raphanus sativus; Polyamines; Auxins; Brassinosteriods; Oxidative stress; Heavy metal stress.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202009000100004

Antioxidant system response induced by aluminum in two rice cultivars
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Ribeiro,Cleberson; Cambraia,José; Peixoto,Paulo Henrique Pereira; Fonseca Júnior,Élcio Meira da.
The antioxidant defense system response was evaluated in two rice cultivars (Oryza sativa L.), Fernandes (CNA-1158) and Maravilha (CNA-6843-1), treated with toxic levels of aluminum. After exposure to aluminum we determined plant growth, H2O2 and O2•- contents, lipid peroxidation, antioxidant enzymes activities and ascorbate and dehydroascorbate contents. Al predominantly accumulated in roots of both cultivars but it reduced root and shoot growth only in the Maravilha cultivar. Treatment with aluminum resulted in a reduction of 84 and 60% in the levels of H2O2 in the roots of the cultivars Maravilha and Fernandes, respectively, and of 26% in the levels of O2•- only in the roots of Fernandes cultivar. Increased lipid peroxidation was observed only in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aluminum; Enzymes; Reactive oxygen species; Oryza sativa; Oxidative stress.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202012000200004

Salt-induced changes in antioxidative enzyme activities in root tissues do not account for the differential salt tolerance of two cowpea cultivars
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Maia,Josemir M; Voigt,Eduardo L; Macêdo,Cristiane E. C; Ferreira-Silva,Sérgio L; Silveira,Joaquim A. G.
The salt stress effect in root growth and antioxidative response were investigated in two cowpea cultivars which differ in salt tolerance in terms of plant growth and leaf oxidative response. Four-day-old seedlings (establishment stage) were exposed to 100 mM NaCl for two days. The roots of the two cultivars presented distinct response in terms of salt-induced changes in elongation and dry weight. Root dry weight was only decreased in Pérola (sensitive) cultivar while root elongation was mainly hampered in Pitiúba (tolerant). Root relative water content remained unchanged under salinity, but root Na+ content achieved toxic levels as revealed by the K+/Na+ ratio in both cultivars. Then, root growth inhibition might be due to ionic toxicity rather than by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidative enzymes; Oxidative stress; Root growth inhibition; Salt stress; Vigna unguiculata.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000200005

Effect of Methyl Jasmonate on antioxidative enzyme activities and on the contents of ROS and H2O2 in Ricinus communis leaves
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Soares,Alexandra Martins dos Santos; Souza,Thiago Freitas de; Jacinto,Tânia; Machado,Olga Lima Tavares.
Jasmonates are a class of plant hormones that mediate various aspects in gene and metabolic regulation, defense, stress responses, reproduction and, possibly, communication. Oxidative stress stimulates synthesis of antioxidant metabolites and enhances antioxidant enzyme activities that could protect plant tissues. The aim of this study was to verify the effects of methyl jasmonate (JAME) treatment on the reactive oxygen species (ROS) and on the activities of H2O2 scavenging enzymes, such as superoxide dismutase (SOD; EC 1.15.1.1), catalase (CAT; EC 1.11.1.6), ascorbate peroxidase (APX EC; 1.11.1.1), and guaiacol peroxidase (GPX; EC 1.11.1.7) in Ricinus communis leaves. The activity of CAT and GPX was transient while SOD activity decreased and APX increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methyl jasmonate; Oxidative stress; Antioxidant enzymatic defenses; Catalase; Superoxide dismutase; Peroxidase; Ricinus comunnis; Signal transduction.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000300001

Physiological and biochemical changes occurring in dwarf-cashew seedlings subjected to salt stress
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Abreu,Carlos E.B. de; Prisco,José T.; Nogueira,Ana R.C.; Bezerra,Marlos A.; Lacerda,Claudivan F. de; Gomes-Filho,Enéas.
The effects of salt stress on some physiological and biochemical traits were evaluated in dwarf-cashew seedlings at the same developmental stage. Seeds were sown in trays containing vermiculite moistened with distilled water or with NaCl solutions having different electrical conductivities: 0.7, 1.8, 6.0, 9.8, 13.4, 17.4 and 20.6 dS m-1. Salinity delayed and inhibited seedling growth and development, particularly in the shoot. Concentrations of Na+ and Cl-, but not of K+, increased with increasing stress severity. With the exception of proline, concentration of organic solutes was only marginally affected by salt stress. Catalase activity in leaves increased slightly as a result of salt stress, whereas guaiacol peroxidase activity was induced only under...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anacardium occidentale; Antioxidant enzymes; Lipid peroxidation; Oxidative stress; Protein pattern; Salt stress.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202008000200003

Effectivity of anti-oxidative enzymatic system on diminishing the oxidative stress induced by aluminium in chickpea (Cicer arietinum L.) seedlings
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Singh,Sushant; Verma,Anil; Dubey,Vikash Kumar.
We explore physiological and biochemical response under increasing aluminium stress at different time interval in chickpea seedlings (Cicer arietinum L.). Germination percentage and root length were found to be highly reduced under increasing metal stress. Aluminium induced oxidative stress and led to fluctuations in antioxidative activity responses. Roots showed higher antioxidative activity compared to shoots. Low concentration of aluminium after a brief treatment induced higher superoxide dismutase (SOD; EC 1.15.1.1), ascorbate peroxidase (APX; EC 1.11.1.11) and guaiacol peroxidase (GPX; EC 1.11.1.7) activity whereas longer duration of treatment led to decrease in these activities. Malondialdehyde concentration indicated higher oxidative damage in roots...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascorbate peroxidases; Heavy metal; Malondialdehyde; Superoxide dismutase; Oxidative stress.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202012000100007

Aspects of nitrogen metabolism in coffee plants
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Carelli,Maria Luiza Carvalho; Fahl,Joel Irineu; Ramalho,José D. Cochicho.
Coffee plants are highly N-demanding plants. Despite the importance of N nutrition for the development, acclimation and yield of coffee plants, there are few reports concerning N metabolism in this species. In this review, our intention is to summarize the information available in the literature and to point out the influence of environmental conditions on N assimilation, as well as comment and discuss some apparently contradictory results and raise and enlighten queries about N assimilation in coffee plants.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea; Irradiance level; Nitrate reductase; Nitrogen fertilization; Oxidative stress; Photoinhbition; Photoprotection; Photosynthesis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100002

Changes in growth, pigment content and antioxidants in the root and leaf tissues of wheat plants under the influence of exogenous salicylic acid
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Sahu,Gopal Krishna; Sabat,Surendra Chandra.
The effect of various concentrations of salicylic acid (SA) on the growth, pigment content and the activity of antioxidants was investigated in the laboratory grown wheat plants. The root and shoot growth was affected at higher concentration of SA in early days of growth. The activities of catalase (CAT), ascorbate peroxidase (APX) and guaicol-specific peroxidase (POX) declined with the application of SA (50, 500 and 1000 µM), the decrease being more pronounced with the increase in SA concentrations both in the root and leaf tissues. On the other hand superoxide dismutase (SOD) activity increased with the application of SA. At low concentrations, SA has no effect on the activities of these enzymes in vitro. Salicylic acid at higher concentrations (5- and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Oxidative stress; Salicylic acid; Triticum aestivum L.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000300005

Physiological and oxidative stress responses of four potato clones to aluminum in nutrient solution
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Tabaldi,Luciane A.; Nicoloso,Fernando T.; Castro,Gabriel Y.; Cargnelutti,Denise; Gonçalves,Jamile F.; Rauber,Renata; Skrebsky,Etiane C.; Schetinger,Maria R.C.; Morsch,Vera M.; Bisognin,Dilson A.
Aluminum toxicity is a serious problem in Brazilian soils and selecting potato clones is an important strategy to produce this crop on these kinds of soils. Potato clones, Macaca, SMIC148-A, Dakota Rose, and Solanum microdontum, were grown in a nutrient solution (pH 4.0) with 0, 50, 100, 150 and 200 mg Al L-1. After 7 d, Al concentration in both root system and shoot of all clones increased linearly with increasing Al levels. Based on relative root growth, S. microdontum and SMIC148-A were considered Al-tolerant clones, whereas Macaca and Dakota Rose were considered Al-sensitive. Shoot growth in Macaca linearly decreased with increasing Al levels. Root H2O2 concentration in both Al-sensitive clones increased with increasing Al supply, whereas in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aluminum toxicity; Antioxidative enzymes; Growth; Oxidative stress; Solanum tuberosum.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000300005

Cadmium toxicity in plants
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Benavides,María P.; Gallego,Susana M.; Tomaro,María L.
Heavy metals are important environmental pollutants and their toxicity is a problem of increasing significance for ecological, evolutionary, nutritional, and environmental reasons. Plants posses homeostatic cellular mechanisms to regulate the concentration of metal ions inside the cell to minimize the potential damage that could result from the exposure to nonessential metal ions. This paper summarizes present knowledge in the field of higher plant responses to cadmium, an important environmental pollutant. Knowledge concerning metal toxicity, including mechanisms of cadmium homeostasis, uptake, transport and accumulation are evaluated. The role of the cell wall, the plasma membrane and the mycorrhizas, as the main barriers against cadmium entrance to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cadmium; Heavy metals; Metal toxicity; Phytochelatins; Oxidative stress.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000100003

Pretreatment with H2O2 in maize seeds: effects on germination and seedling acclimation to salt stress
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Gondim,Franklin A; Gomes-Filho,Enéas; Lacerda,Claudivan F; Prisco,José Tarquinio; Azevedo Neto,André D; Marques,Elton C.
The aim of this study was to evaluate the effects of H2O2 on germination and acclimation of maize plants subject to salt stress. Three experiments using BRS3003 seeds, a triple hybrid of maize, were carried out in a growth room and in greenhouse. In the first experiment, H2O2 accelerated the germination percentage of seeds at 100 mM, but not at 500 mM. In the second experiment, the pretreatment of seeds was observed to induce a pronounced increase in ascorbate peroxidase (APX) and catalase (CAT) enzyme activity after 30 h of soaking in H2O2. It was also observed that guaiacol peroxidase (GPX) activity was smaller in the seeds soaked in H2O2 for 12, 24, 30, 36 and 42 h, in relation to those soaked in distilled water. The superoxide dismutase (SOD) activity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant enzymes; Hydrogen peroxide; Oxidative stress; Salinity stress; Zea mays.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000200004

Water relations and some aspects of leaf metabolism of Jatropha curcas young plants under two water deficit levels and recovery
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Arcoverde,Gabriela B.; Rodrigues,Bruno M.; Pompelli,Marcelo F.; Santos,Mauro G.
Stomatal conductance (g s), transpiration (E), relative water content (RWC), superoxide dismutase activity (SOD), malondialdehyde (MDA), leaf carbohydrate soluble (LCS), free amino acids (FAA) and total protein (TP) content were measured under mild and severe drought stress in young potted Jatropha curcas L. in the greenhouse. The plants were kept for 12 days under three different water regimes, control (well hydrated), 50% irrigation control (mild stress) and 25% (severe stress), after rehydration were measured on the second and fourth day. Both mild and severe stress decreased g s and E; however, only severe stress reduced LCS, FAA and TP content and increased SOD activity (70%) and MDA content (60%) compared with the control. Moreover, under these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodiesel; Drought tolerance; MDA; Oxidative stress; ROS; Water deficit.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000200004

Antioxidant-enzymatic system of two sorghum genotypes differing in salt tolerance
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Costa,Paulo Henrique Alves da; Neto,André Dias de Azevedo; Bezerra,Marlos Alves; Prisco,José Tarquinio; Gomes-Filho,Enéas.
Two forage sorghum genotypes were studied: CSF18 (salt-sensitive) and CSF20 (salt-tolerant). Shoot growth reduction as a result of salt stress was stronger in the salt sensitive genotype compared to the salt tolerant one. When the two genotypes were subjected to salt stress (75 mM NaCl) no significant change in lipid peroxidation was observed. However, salt stress induced increases in superoxide dismutase and catalase activities in both genotypes. These salt-induced increases were higher in the salt-tolerant genotype. Peroxidase activity was differentially affected by salt stress in the two genotypes. The activities of these peroxidases were decreased by salt stress in the salt-sensitive genotype and increased in the salt-tolerant genotype. In addition,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sorghum bicolor; Antioxidant-enzymatic system; Lipid peroxidation; Oxidative stress; Salinity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000400003

Photo-oxidative stress in coconut seedlings: early events to leaf scorching and seedling death
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Naresh,Kumar S.; Kasturi Bai,K.V.
Experiments were conducted on coconut seedlings to delineate events of photo-oxidative stress damage. Studies on chlorophyll fluorescence indicated a clear case of excess light energy under high light conditions causing stress to coconut seedlings raised under coconut palms. Quantum yield of photo-chemistry of leaflets exposed to high light was significantly less than those under shade. Seedlings exposed to high light and then shifted to shade have shown significant improvement in quantum yield. Excess light energy harvested by chlorophyll antenna caused high non-photochemical quenching resulting in production of biologically toxic super oxide, hydrogen peroxide and hydroxyl radicals. It is apparent that photoinhibition of photosynthesis takes place due to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cocos nucifera; Chlorophyll fluorescence; Oxidative stress; Photosynthesis; Lipid peroxidation; Gas exchange; Proteins.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202009000300006

Antioxidant and other biochemical defense responses of Macrotyloma uniflorum (Lam.) Verdc. (Horse gram) induced by high temperature and salt stress
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Naji,Khalid Mohammed; Devaraj,V. R.
High temperature and salinity are the major ecological factors challenging crop productivity in the arid and semiarid regions of the world. Effects of high temperature (43-45°C) and salt stress (0.6 M) on Macrotyloma uniflorum (Lam.) Verdc. (Horse gram), were evaluated in terms of antioxidants and antioxidant enzymes. Both treatments caused typical stress responses in this tropical leguminosae. Oxidative stress indicators such as H2O2, TBARS, and proline were significantly elevated. Similarly, the antioxidant enzymes superoxide dismutase (SOD; EC 1.15.1.1), guaiacol peroxidase (POX; EC 1.11.1.7) and acid phosphates (AP; EC 3.1.3.2) were significantly elevated while catalase (CAT; EC 1.11.1.6) was reduced. These treatments had contrasting effects on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant enzymes; Horse gram; Isozymes; Oxidative stress; Salinity; Stress markers.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000300002

Seed germination and antioxidant enzyme activity in seedlings of diploid and tetraploid bahiagrass under water restriction
Provedor de dados: Ciência Rural
Autores: Bortolin,Gabriel Streck; Salinas-Arcienega,Alfredo José; Galviz-Fajardo,Yutcelia Carolina; Amarante,Luciano do; Pedroso,Carlos Eduardo da Silva; Oliveira,João Carlos Pinto; Köpp,Mauricio Marini; Martins,Andrea Bicca Noguez; Tunes,Lilian Vanussa Madruga de.
ABSTRACT: The objective of the following research was to investigate the physiological responses of two bahiagrass genotypes (Paspalum notatum Flügge) submitted to water restriction, induced by polyethylene glycol (PEG6000), during seed germination. Seeds of cv. Pensacola (diploid) and Bagual ecotype (tetraploid) were placed on paper moistened with osmotic solutions at potentials of 0.0; -0.1; -0.2 and -0.3 MPa. Composed of two subsamples of 100 seeds and four experimental units per treatment, the germination test was conducted in a germination chamber under alternating temperatures of 30 °C (8 hours) and 20 °C (16 hours), with presence of light during the higher temperature. Final germination percentage and germination speed index of the seeds, as well as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paspalum notatum Flügge; Ecotype; Polyethylene glycol; Drought stress; Oxidative stress.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000500202

Changes caused by heavy metals in micronutrient content and antioxidant system of forage grasses used for phytoremediation: an overview
Provedor de dados: Ciência Rural
Autores: Rabêlo,Flávio Henrique Silveira; Borgo,Lucélia.
ABSTRACT: An increase in the content of heavy metals in the environment causes many socio-environmental problems, and phytoremediation is a tool to reduce the environmental impact caused by these elements, with prospects for the use of forage grasses. This group of plants features characteristics for the environment-decontamination process, but further studies are necessary about the damages caused by heavy metals on the uptake of cationic micronutrients and on the antioxidant system, which are essential processes for the growth of plants in contaminated sites. Exposure of forage grasses to heavy metals results in a lower content of Mn in the shoots of almost all plants, but the contents of Cu, Fe, and Zn vary according to heavy metal and forage grass....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomass; Nutritional status; Oxidative stress; Environmental pollution.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000801368

Changes in oxidative markers, endogenous antioxidants and activity of the enzyme acetylcholinesterase in farmers exposed to agricultural pesticides - a pilot study
Provedor de dados: Ciência Rural
Autores: Murussi,Camila; Horn,Roberta Cattaneo; Santi,Adriana; Clasen,Barbara Estevão; Reis,Gabriel; Souza,Daniela; Bortolotto,Josiane Woutheres; Manfio,Candida Elisa; Loro,Vania Lucia.
Agricultural practices are directly related to the use of pesticides, which indiscriminately and without due care may contribute to the occurrence of numerous intoxications. Several studies have demonstrated the relationship of certain pesticides and the occurrence of oxidative stress and therefore, in recent years have developed methods of analysis of several biomarkers of cellular damage that can be measured and quantified. In this context, the objective of this study was to evaluate the possible changes in biochemical markers: glutamic aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine, urea, total protein, and oxidative markers such as lipid peroxidation, damage to proteins and the activity of the enzymes acetylcholinesterase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rarmers; Oxidative stress; TBARS; Protein carbonyls.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000701186

Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis
Provedor de dados: Ciência e Agrotecnologia
Autores: Venâncio,Vinícius Paula; Ferreira,Eric Batista; Brigagão,Maísa Ribeiro Pereira Lima; Paula,Fernanda Borges de Araújo; Barbisan,Luis Fernando; Azevedo,Luciana.
A. crassiflora Mart. a Brazilian savannah fruit, is a source of phytochemical compounds that possess a wide array of biological activities, including free radical scavenging. This native fruit proved to potentialize the mutagenic process in previous in vivo investigations. The aim of the present study was to investigate the effects of A. crassiflora Mart. pulp intake on colonic cell proliferation and on the development of Aberrant Crypt Foci (ACF) in male Wistar rats. The animals were fed with either a commercial diet or a diet supplemented with A. crassiflora Mart. pulp mixed in 1%, 10% or 20% (w/w) for 4 weeks or 20 weeks. The carcinogen 1,2-dimethylhydrazine dihydrochloride (4 doses, 40 mg kg-1 each) was used to induce colonic ACF. After euthanasia, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aberrant crypt foci; Brazilian savannah; Dimethylhydrazine; Immunohistochemistry; Oxidative stress.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542013000400008

Water restriction in different phenological stages and increased temperature affect cowpea production
Provedor de dados: Ciência e Agrotecnologia
Autores: Barros,Juliane Rafaele Alves; Guimarães,Miguel Julio Machado; Simões,Welson Lima; Melo,Natoniel Franklin de; Angelotti,Francislene.
ABSTRACT Water deficit and high temperatures are abiotic factors that most limit plant growth and development. However, its effects depend on crop development stage and on stress duration and intensity. Thus, the objective of was to evaluate the development of cowpea subjected to water restriction in different phenological stages and to increase in air temperature. The experiment was conducted with the cultivar ‘Carijó’, in growth chambers, in a 4 x 3 x 2 factorial arrangement, corresponding to levels of water availability (25, 50, 75, and 100%,), phenological stages (vegetative, flowering and pod filling) and temperature regimes (T°1: 20-26-33 °C e T°2: 24.8-30.8-37.8 °C), respectively. Reduction of water availability in the vegetative and flowering...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abiotic stress; Oxidative stress; Phenology; Vigna unguiculata.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542021000100200

ANTIOXIDANT ENZYME ACTIVITY AND FRESH-CUT ARRACACHA QUALITY
Provedor de dados: Ciência e Agrotecnologia
Autores: Vilela,Hêmina Carla; Goulart,Patrícia de Fátima Pereira; Souza,Kamila Rezende Dázio de; Boas,Ana Carolina Vilas; Roda,Jane Silva; Oliveira,Roseane Maria Evangelista de.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arracacia xanthorrhiza; Minimally processed foods; Oxidative stress; Useful life; Physicochemical aspects.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542015000300276