Consulta: subjectFacets:"PARP"
Registros recuperados: 4
Data/hora: 17/06/2024 15:36:06

ADP-ribosylation: from molecular mechanisms to human disease
Provedor de dados: 74
Autores: Hoch,Nicolas C.; Polo,Luis M.
Abstract Post-translational modification of proteins by ADP-ribosylation, catalysed by poly (ADP-ribose) polymerases (PARPs) using NAD+ as a substrate, plays central roles in DNA damage signalling and repair, modulates a range of cellular signalling cascades and initiates programmed cell death by parthanatos. Here, we present mechanistic aspects of ADP-ribose modification, PARP activation and the cellular functions of ADP-ribose signalling, and discuss how this knowledge is uncovering therapeutic avenues for the treatment of increasingly prevalent human diseases such as cancer, ischaemic damage and neurodegeneration.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ADP ribose; PARP; DNA damage response; DNA repair; Parthanatos.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200304

Persistent double strand break accumulation does not precede cell death in an Olaparib-sensitive BRCA-deficient colorectal cancer cell model
Provedor de dados: 74
Autores: Paviolo,Natalia Soledad; Vega,María Belén de la; Pansa,María Florencia; García,Iris Alejandra; Calzetta,Nicolás Luis; Soria,Gastón; Gottifredi,Vanesa.
Abstract The poly (adenosine diphosphate (ADP)-ribosyl) polymerase inhibitors (PARPi) selectively kill cancer cells with BRCA1 or BRCA2 (BRCA)-mutations. It has been proposed that cell death induction after PARPi depends on unrepaired double strand breaks (DSBs) that accumulate due to the homologous recombination deficiency of BRCA-mutated cells. Such accumulation of DSBs is inferred mainly from the high levels of DNA damage markers like phosphorylated histone H2AX. Herein, we developed a model of isogenic cell lines to show that depletion of BRCA causes PARPi-triggered cell death, replication stress (phosphorylated-H2AX and 53BP1 foci), and genomic instability. However, persistent DSBs accumulation was not detected under the same experimental conditions....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GammaH2AX; Alternative end joining; Non-homologous end joining; Homologous recombination; PARP.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200303

Kinetoplast DNA and Procyclic Acidic Repetitive Protein A-α Gene of Trypanosoma evansi
Provedor de dados: 19
Autores: Inoue, Noboru; Honzako, Y.; Hirumi, K.; Xuan, Xuenan; Agatsuma, T.; Nagasawa, Hideyuki; Mikami, Takeshi; Hirumi, Hiroyuki.
Palavras-chave: PARP; KDNA; Trypanosoma evansi; T. brucei.
Ano: 1998
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/276

Large-scale production and purification of recombinant protein from an insect cell/baculovirus system in Erlenmeyer flasks: application to the chicken poly(ADP-ribose) polymerase catalytic domain
Provedor de dados: 56
Autores: Miranda,E.A.; de-Murcia,G.; Ménissier-de-Murcia,J.
A simple and inexpensive shaker/Erlenmeyer flask system for large-scale cultivation of insect cells is described and compared to a commercial spinner system. On the basis of maximum cell density, average population doubling time and overproduction of recombinant protein, a better result was obtained with a simpler and less expensive bioreactor consisting of Erlenmeyer flasks and an ordinary shaker waterbath. Routinely, about 90 mg of pure poly(ADP-ribose) polymerase catalytic domain was obtained for a total of 3 x 109 infected cells in three liters of culture
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PARP; Insect cells; Baculovirus; Overproduction.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800002