Consulta: subjectFacets:"PCR-RAPD"
Registros recuperados: 3
Data/hora: 13/06/2024 08:46:04

Molecular Epidemiology of Cystic Echinococcosis: Genotypic Characterization in Humans and Different Livestock
Provedor de dados: 77
Autores: Manterola,Carlos; Otzen,Tamara.
Echinococcus Granulosus (EG) is the major cause of cystic echinococcosis in humans and livestock in the world. In Chile is a zoonosis of great importance. The most frequently affected geographic areas are the Regions of Aysén, Los Rios, Los Lagos, Coquimbo and the Araucanía. Hence, it was discovered that in endemic areas of hydatidosis there could be several strains and genotypes of EG. In addition, there is evidence that some strains and genotypes are more infectious for human beings than others. This interesting phenomenon of the biology of EG has been studied using molecular biology techniques based on polymerase chain reaction (PCR) and DNA sequence analysis, which has made it possible to characterize the cestode species complex called EG sensu lato (s...
Tipo: Journal article
Palavras-chave: Echinococcus granulosus; Genotypes; Cytochrome c oxidase subunit I; PCR-RAPD.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400048

Genética de poplaciones de Atta sexdens rubropilosa (Hymenoptera: Formicidae)
Provedor de dados: 37
Autores: BELIZÁRIO CANTAGALLI,LIRIANA; APARECIDA MANGOLIN,CLAUDETE; COLLA RUVOLO TAKASUSUKI,MARIA CLAUDIA.
La variabilidad genética de las hormigas Atta sexdens rubropilosa colectadas en cinco lugares distintos de Brasil fueron evaluados por la técnica PCR-RAPD. Un total de 15 primers produjeron 148 fragmentos, de los cuales 123 fueron polimórficos, lo que corresponden al 83,11 %. La estimación de la diversidad genética por el índice de Shannon fue 0,3836 y el desviación estándar fue de ± 0,2335. Estos valores demuestran una alta diversidad genética. El valor de G ST fue 0,2372 y ΦPT = 0,184 lo que indica que las poblaciones están moderadamente diferenciadas y que el 82 % de la variación obtenida se produce dentro de las poblaciones. Aunque la prueba de Mantel ha demostrado una correlación entre la distancia genética y geográfica se observó que Ivatuba e Itambé...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hormigas cortadoras; PCR-RAPD; Variabilidad genética.
Ano: 2013
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2013000100013

Population genetics of the sugarcane borer Diatraea saccharalis (Fabr.) (Lepidoptera: Crambidae)
Provedor de dados: 1
Autores: Lopes, Denise Alves; Universidade Estadual de Maringá; Cantagalli, Liriana Belizário; Universidade Estadual de Maringá; Stuchi, Ana Lucia Paz Barateiro; Universidade Estadual de Maringá; Mangolin, Claudete Aparecida; Universidade Estadual de Maringá; Ruvolo-Takasusuki, Maria Claudia Colla; Universidade Estadual de Maringá.
Diatraea saccharalis is the principal pest of sugarcane in Brazil and is found throughout the sugarcane crop. Information about its population genetics is scarce, but population genetic analysis is of particular importance as a basis for a successful pest control program. Pest control requires a constant evaluation of genetic variability so that appropriate strategies can be employed. In this study, the structure of D. saccharalis populations in sugarcane crops was analyzed with PCR-RAPD (Polymerase Chain Reaction-Random Amplified Polymorphic DNA) markers. Samples were collected from four sugarcane fields in ParanáState and one in São PauloState. Twelve PCR-RAPD primers were used. The analysis produced 216 fragments, with a 99.54% polymorphism rate. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5.01.02.02-8 and integrated using chemical and biological control.  molecular marker; Polymorphism; PCR-RAPD; Sugarcane pest Fitossanidade.
Ano: 2014
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/16211