Consulta: subjectFacets:"PCR-REA"
Registros recuperados: 2
Data/hora: 12/06/2024 14:09:52

First identification of Mycobacterium avium paratuberculosis sheep strain in Argentina
Provedor de dados: Brazilian Journal of Microbiology
Autores: Travería,G.E.; Zumarraga,M.; Etchechoury,I.; Romano,M.I.; Cataldi,A.; Alvarado Pinedo,M.F.; Pavlik,I.; Pribylova,R.; Romero,J.R.
We here identified for the first time the presence of Mycobacterium avium paratuberculosis (MAP) sheep (S) strain in Argentina. IS900 polymerase chain reaction (PCR) was positive. The S strain was compared with MAP cattle (C) strains by using IS1311 PCR-restriction endonuclease analysis (PCR-REA), multiplex PCR and restriction fragment length polymorphism (RFLP) analysis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paratuberculosis; Sheep; Typing; PCR; PCR-REA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300037

An improved method for characterization of the mutation associated to porcine stress syndrome by PCR amplification followed by restriction analysis
Provedor de dados: Ciência Rural
Autores: Luerce,Tessália Diniz; Galli,Vanessa; Cerqueira,Gustavo Maia; Simionatto,Simone; Dellagostin,Odir Antônio.
A mutation in the gene coding for the ryanodine receptor 1 (RYR1), also known as halothane (hal) gene or swine stress gene, is associated to the porcine stress syndrome (PSS). Detection of the mutation is normally accomplished by PCR amplification of an 81bp fragment of the hal gene, followed by digestion with the HhaI restriction endonuclease. Wild-type allele (N) is cut in two fragments, whereas the mutant allele (n) is not digested by the restriction enzyme. Electrophoresis of the digested DNA on agarose gel and ethidium bromide staining allows the reading of the result. The correct interpretation is difficult due to the small size of the DNA fragments. In this study we designed a new set of primers for amplification of a 144bp fragment that facilitates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR-REA; Plucked hair; Swine stress gene; PSE pork.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000500044