Consulta: subjectFacets:"PI3K/AKT pathway"
Registros recuperados: 3
Data/hora: 09/06/2024 02:30:03

Ginsenoside Rg1 protects human renal tubular epithelial cells from lipopolysaccharide-induced apoptosis and inflammation damage
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ni,X.J.; Xu,Z.Q.; Jin,H.; Zheng,S.L.; Cai,Y.; Wang,J.J.
Ginsenoside Rg1, one of the most notable active components of Panax ginseng, has been widely reported to exert anti-inflammatory actions. This study aimed to reveal whether ginsenoside Rg1 also exhibits beneficial roles against lipopolysaccharide (LPS)-induced apoptosis and inflammation in human renal tubular epithelial cells, and to evaluate the potential role of the component on tubulointerstitial nephritis treatment. HK-2 cells were treated with various doses of ginsenoside Rg1 (0, 50, 100, 150, and 200 μM) in the absence or presence of 5 μg/mL LPS. Thereafter, CCK-8 assay, flow cytometry, western blot, migration assay, reactive oxygen species (ROS) assay, and ELISA were carried out to respectively assess cell viability, apoptosis, migration, ROS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ginsenoside Rg1; LPS; Apoptosis; Inflammation; PI3K/AKT pathway; NF-κB pathway.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200608

lncRNA CCAT1 promotes cell proliferation, migration, and invasion by down-regulation of miR-143 in FTC-133 thyroid carcinoma cell line
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Yang,Tianzheng; Zhai,Hongyan; Yan,Ruihong; Zhou,Zhenhu; Gao,Lei; Wang,Luqing.
Thyroid cancer is a common malignant tumor. Long non-coding RNA colon cancer-associated transcript 1 (lncRNA CCAT1) is highly expressed in many cancers; however, the molecular mechanism of CCAT1 in thyroid cancer remains unclear. Hence, this study aimed to investigate the effect of CCAT1 on human thyroid cancer cell line FTC-133. FTC-133 cells were transfected with CCAT1 expressing vector, CCAT1 shRNA, miR-143 mimic, and miR-143 inhibitor, respectively. After different treatments, cell viability, proliferation, migration, invasion, and apoptosis were measured. Moreover, the regulatory relationship of CCAT1 and miR-143, as well as miR-143 and VEGF were tested using dual-luciferase reporter assay. The relative expressions of CCAT1, miR-143, and VEGF were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyroid cancer; CCAT1; MiR-143; VEGF; PI3K/AKT pathway; MAPK pathway.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600615

miR-26a-5p protects against myocardial ischemia/reperfusion injury by regulating the PTEN/PI3K/AKT signaling pathway
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Xing,Xiaowei; Guo,Shuang; Zhang,Guanghao; Liu,Yusheng; Bi,Shaojie; Wang,Xin; Lu,Qinghua.
Reperfusion strategies in acute myocardial infarction (AMI) can cause a series of additional clinical damage, defined as myocardial ischemia/reperfusion (I/R) injury, and thus there is a need for effective therapeutic methods to attenuate I/R injury. miR-26a-5p has been proven to be an essential regulator for biological processes in different cell types. Nevertheless, the role of miR-26a-5p in myocardial I/R injury has not yet been reported. We established an I/R injury model in vitro and in vivo. In vitro, we used cardiomyocytes to simulate I/R injury using hypoxia/reoxygenation (H/R) assay. In vivo, we used C57BL/6 mice to construct I/R injury model. The infarct area was examined by TTC staining. The level of miR-26a-5p and PTEN was determined by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute myocardial infarction; MiR-26a-5p; PTEN; Apoptosis; PI3K/AKT pathway; Myocardial ischemia/reperfusion injury.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200601