Consulta: subjectFacets:"PTEN"
Registros recuperados: 6
Data/hora: 10/06/2024 15:02:32

Exosomes promote cetuximab resistance via the PTEN/Akt pathway in colon cancer cells
Provedor de dados: 56
Autores: Zhang,S.; Zhang,Y.; Qu,J.; Che,X.; Fan,Y.; Hou,K.; Guo,T.; Deng,G.; Song,N.; Li,C.; Wan,X.; Qu,X.; Liu,Y.
Cetuximab is widely used in patients with metastatic colon cancer expressing wildtype KRAS. However, acquired drug resistance limits its clinical efficacy. Exosomes are nanosized vesicles secreted by various cell types. Tumor cell-derived exosomes participate in many biological processes, including tumor invasion, metastasis, and drug resistance. In this study, exosomes derived from cetuximab-resistant RKO colon cancer cells induced cetuximab resistance in cetuximab-sensitive Caco-2 cells. Meanwhile, exosomes from RKO and Caco-2 cells showed different levels of phosphatase and tensin homolog (PTEN) and phosphor-Akt. Furthermore, reduced PTEN and increased phosphorylated Akt levels were found in Caco-2 cells after exposure to RKO cell-derived exosomes....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cetuximab; Exosome; PTEN; Akt; Colon cancer.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100609

Puerarin inhibits hepatocellular carcinoma invasion and metastasis through miR-21-mediated PTEN/AKT signaling to suppress the epithelial-mesenchymal transition
Provedor de dados: 56
Autores: Zhou,Yuan; Xue,Ruifeng; Wang,Jinglin; Ren,Haozhen.
Hepatocellular carcinoma (HCC) is one of the most common primary malignant tumors of the liver worldwide. Liver resection and transplantation are currently the only effective treatments; however, recurrence and metastasis rates are still high. Previous studies have shown that the epithelial-mesenchymal transition (EMT) is a key step in HCC invasion and metastasis. Inhibition of EMT has become a new therapeutic strategy for tumors. Recently, puerarin, a well-characterized component of traditional Chinese medicine, has been isolated from Pueraria radix and exerts positive effects on many diseases, particularly cancers. In this study, CCK-8, EdU immunofluorescence, colony formation, wound healing, and migration assays were used to detect the effects of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatocellular carcinoma; Metastasis; Epithelial-mesenchymal transition; PTEN; MiR-21.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400611

FSH receptor binding inhibitor up-regulates ARID1A and PTEN genes associated with ovarian cancers in mice
Provedor de dados: 56
Autores: Gong,Zhuandi; Shen,Xiaoyun; Yang,Juan; Yang,Kun; Bai,Shengju; Wei,Suocheng.
Experiments were conducted to determine if the follicle-stimulating hormone (FSH) receptor binding inhibitor (FRBI) impacts the expression levels of AT-rich interactive domain-containing protein 1A (ARID1A) and phosphatase and tensin homolog (PTEN) in ovaries and blood, as well as expressions of follicle-stimulating hormone cognate receptor (FSHR) gene and proteins. Mice in FRBI-10, FRBI-20, FRBI-30, and FRBI-40 groups were intramuscularly injected with 10, 20, 30, and 40 mg FRBI/kg, respectively, for five consecutive days. Western blotting and qRT-PCR were utilized to determine expression levels of ARID1A and PTEN proteins and mRNAs. Serum ARID1A and PTEN concentrations of the FRBI-40 group were higher than the control group (CG) and FSH group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ARID1A; PTEN; FSH receptor binding inhibitor; Ovarian cancer.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700604

Prevalence of ERG expression and PTEN loss in a Brazilian prostate cancer cohort
Provedor de dados: 56
Autores: Morais,C.E.; Gurgel,D.C.; Teixeira,A.C.; Mattos,T.V. Arruda; Silva,A.V. Alves da; Tavora,F.
PTEN is the most commonly inactivated tumor suppressor gene in primary prostate cancer (PCa) and its loss is associated with poor clinical outcomes. ERG rearrangement is a genomic alteration frequently found in PCa and its prognostic significance has yielded mixed results. Although the association of PTEN and ERG biomarkers has potential impact on clinical outcomes, studies examining the two genes simultaneously are scarce in Brazilian populations. In this study, we retrospectively examined the relationship between ERG expression and PTEN loss in 119 surgically treated prostate cancer patients from Northeastern Brazil through immunohistochemical analysis. ERG expression was found in 41.0% (48/117) of cases and the loss of PTEN detected in 38.1% (40/105) of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ERG expression; PTEN; Biomarker; Radical prostatectomy; Prostatic carcinoma.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200610

Up-regulated miR-106b inhibits ox-LDL-induced endothelial cell apoptosis in atherosclerosis
Provedor de dados: 56
Autores: Zhang,Yunqing; Wang,Li; Xu,Jie; Kong,Xiaomei; Zou,Lin.
This research aimed to explore the molecular mechanism of microRNA (miR)-106b in cell apoptosis of atherosclerosis (AS). Human aortic endothelial cells (HAECs) were divided into control group, oxidized-low-density lipoproteins (ox-LDL) group, miR-106b NC+ox-LDL group, miR-106b mimics+ox-LDL group, miR-106b mimics+PTEN+ox-LDL group, and miR-106b mimics+empty+ox-LDL group. Real-time fluorescence quantitative polymerase chain reaction, cholecystokinin, TdT-mediated biotinylated nick end-labeling assay, luciferase reporter gene assay, and flow cytometry analysis were performed to determine the morphology, proliferation, and apoptosis in HSECs. Moreover, the levels of phosphatase and tensin homolog deleted on chromosome 10 (PTEN), Bcl-2, p-P13K, and p-AKT in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Ox-LDL; MiR-106b; PTEN; P13K/AKT; Endothelial cell apoptosis.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300610

miR-26a-5p protects against myocardial ischemia/reperfusion injury by regulating the PTEN/PI3K/AKT signaling pathway
Provedor de dados: 56
Autores: Xing,Xiaowei; Guo,Shuang; Zhang,Guanghao; Liu,Yusheng; Bi,Shaojie; Wang,Xin; Lu,Qinghua.
Reperfusion strategies in acute myocardial infarction (AMI) can cause a series of additional clinical damage, defined as myocardial ischemia/reperfusion (I/R) injury, and thus there is a need for effective therapeutic methods to attenuate I/R injury. miR-26a-5p has been proven to be an essential regulator for biological processes in different cell types. Nevertheless, the role of miR-26a-5p in myocardial I/R injury has not yet been reported. We established an I/R injury model in vitro and in vivo. In vitro, we used cardiomyocytes to simulate I/R injury using hypoxia/reoxygenation (H/R) assay. In vivo, we used C57BL/6 mice to construct I/R injury model. The infarct area was examined by TTC staining. The level of miR-26a-5p and PTEN was determined by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute myocardial infarction; MiR-26a-5p; PTEN; Apoptosis; PI3K/AKT pathway; Myocardial ischemia/reperfusion injury.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200601