Consulta: subjectFacets:"Pathogenesis"
Registros recuperados: 26
Data/hora: 08/06/2024 08:25:38

Glutathione S-transferase mu 1 (GSTM1) and theta 1 (GSTT1) genetic polymorphisms and atopic asthma in children from Southeastern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Lima,Carmen Silvia Passos; Néri,Iramaia Angélica; Lourenço,Gustavo Jacob; Faria,Isabel Cristina Jacinto; Ribeiro,José Dirceu; Bertuzzo,Carmen Silvia.
Xenobiotics can trigger degranulation of eosinophils and mast cells. In this process, the cells release several substances leading to bronchial hyperactivity, the main feature of atopic asthma (AA). GSTM1 and GSTT1 genes encode enzymes involved in the inactivation of these compounds. Both genes are polymorphic in humans and have a null variant genotype in which both the gene and corresponding enzyme are absent. An increased risk for disease in individuals with the null GST genotypes is therefore, but this issue is controversial. The aim of this study was to investigate the influence of the GSTM1 and GSTT1 genotypes on the occurrence of AA, as well as on its clinical manifestations. Genomic DNA from 86 patients and 258 controls was analyzed by polymerase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atopic asthma; Pathogenesis; GSTM1 gene; GSTT1 gene.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300007

Bacterial diseases in marine bivalves
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Travers, Marie-agnes; Miller, Katherine Boettcher; Roque, Ana; Friedman, Carolyn S.
Bivalve aquaculture is seriously affected by many bacterial pathogens that cause high losses in hatcheries as well as in natural beds. A number of Vibrio species, but also members of the genera Nocardia and Roseovarius, are considered important pathogens in aquaculture. The present work provides an updated overview of main diseases and implicated bacterial species affecting bivalves. This review focuses on aetiological agents, their diversity and virulence factors, the diagnostic methods available as well as information on the dynamics of the host-parasite relationship.
Tipo: Text
Palavras-chave: Vibrio; Nocardia; Roseovarius; Rickettsia; Pathogenesis; Diagnostic.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00274/38532/37049.pdf

Species-specific mechanisms of cytotoxicity toward immune cells determine the successful outcome of Vibrio infections
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rubio, Tristan; Oyanedel, Daniel; Labreuche, Yannick; Toulza, Eve; Luo, Xing; Bruto, Maxime; Chaparro, Cristian; Torres, Marta; De Lorgeril, Julien; Haffner, Philippe; Vidal-dupiol, Jeremie; Lagorce, Arnaud; Petton, Bruno; Mitta, Guillaume; Jacq, Annick; Le Roux, Frederique; Charriere, Guillaume; Destoumieux-garzon, Delphine.
Vibrio species cause infectious diseases in humans and animals, but they can also live as commensals within their host tissues. How Vibrio subverts the host defenses to mount a successful infection remains poorly understood, and this knowledge is critical for predicting and managing disease. Here, we have investigated the cellular and molecular mechanisms underpinning infection and colonization of 2 virulent Vibrio species in an ecologically relevant host model, oyster, to study interactions with marine Vibrio species. All Vibrio strains were recognized by the immune system, but only nonvirulent strains were controlled. We showed that virulent strains were cytotoxic to hemocytes, oyster immune cells. By analyzing host and bacterial transcriptional...
Tipo: Text
Palavras-chave: T6SS; Toxin; Dual RNA-seq; Cytolysis; Pathogenesis.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00507/61837/65900.pdf

Bacterial disease in marine bivalves, a review of recent studies: Trends and evolution
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Paillard, Christine; Leroux, Frederique; Borrego, Juan.
The main microbial diseases affecting marine cultured bivalves have been revised on the basis of the etiologic agents, pathogenesis and pathogenicity. Several recent bivalve-interaction models have been studied, including Pecten larvae-Vibrio pectinicida, brown ring disease, juvenile oyster disease, Pacific oyster nocardiosis and summer mortalities of oysters. In addition, the taxonomy and phylogeny of new potential bivalve pathogens and their virulence factors have been established. Facing the difficulty of identifying bacterial strains associated with molluscan diseases (mainly vibriosis), polyphasic approaches have been developed to correlate the phenotype and genotype of potential pathogens. By evaluating likely virulence mechanisms, developing...
Tipo: Text
Palavras-chave: Diagnostic tools; Virulence factors; Pathogenicity; Pathogenesis; Etiology; Bivalve mollusc; Vibriosis.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-407.pdf

Combination of a pesticide exposure and a bacterial challenge: In vivo effects on immune response of Pacific oyster, Crassostrea gigas (Thunberg)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Gagnaire, Beatrice; Gay, Melanie; Huvet, Arnaud; Daniel, Jean-yves; Saulnier, Denis; Renault, Tristan.
To assess the impact of pollution induced by pesticides on Pacific oyster, Crassostrea gigas, health in France, in vivo effects of combined pesticide exposure and bacterial challenge on cell activities and gene expression in hemocytes were tested using flow cytometry and real-time PCR. As a first step, an in vivo model of experimental contamination was developed. Pacific oysters were exposed to a mixture of eight pesticides (atrazine, glyphosate, alachlor, metolachlor, fosetyl-alumimium, terbuthylazine, diuron and carbaryl) at environmentally relevant concentrations over a 7-day period. Hemocyte parameters (cell mortality, enzyme activities and phagocytosis) were monitored using flow cytometry and gene expression was evaluated by real-time PCR (RT-PCR)....
Tipo: Text
Palavras-chave: Gene expression; Flow cytometry; Vibrio splendidus; Pathogenesis; Phagocytosis; Hemocyte; Pesticides; Bivalve immunity; Crassostrea gigas; Pacific oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3022.pdf

Cellular and molecular hemocyte responses of the Pacific oyster, Crassostrea gigas, following bacterial infection with Vibrio aestuarianus strain 01/32
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Labreuche, Yannick; Lambert, Christophe; Soudant, Philippe; Boulo, Viviane; Huvet, Arnaud; Nicolas, Jean-louis.
The strategies used by bacterial pathogens to circumvent host defense mechanisms remain largely undefined in bivalve molluscs. In this study, we investigated experimentally the interactions between the Pacific oyster (Crassostrea gigas) immune system and Vibrio aestuarianus strain 01/32, a pathogenic bacterium originally isolated from moribund oysters. First, an antibiotic-resistant V. aestuarianus strain was used to demonstrate that only a limited number of bacterial cells was detected in the host circulatory system, suggesting that the bacteria may localize in some organs. Second, we examined the host defense responses to V. aestuarianus at the cellular and molecular levels, using flow-cytometry and real-time PCR techniques. We showed that hemocyte...
Tipo: Text
Palavras-chave: Crassostrea gigas; Oyster; Pathogenesis; Vibrio aestuarianus; Bivalve immunity.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2182.pdf

Vibriosis induced by experimental cohabitation in Crassostrea gigas: Evidence of early infection and down-expression of immune-related genes
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: De Decker, Sophie; Saulnier, Denis.
The understanding of reciprocal interactions between Crassostrea gigas and Vibrio sp., whether these be virulent or avirulent, is vital for the development of methods to improve the health status of cultured oysters. We describe an original non-invasive experimental infection technique using cohabitation, designed to explore these interactions. Using real-time PCR techniques we examined the dynamics of virulent and avirulent Vibrio sp. in oyster hemolymph and tank seawater, and made a parallel study of the expression of four genes involved in oyster immune defense: Cg-BPI, Cg-EcSOD, Cg-IκB, Cg-TIMP.  No mortality occurred in control animals, but oysters put in cohabitation for 2–48 h with animals previously infected by two Vibrio pathogens suffered...
Tipo: Text
Palavras-chave: Oyster-Vibrio interactions; Pathogenesis; Immune response; Non-invasive experimental challenge; Real-time PCR.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00025/13604/10681.pdf

Use of Cavia porcellus (guinea pigs) as an experimental model for Leishmania (Viannia) braziliensis
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Paula,C. D. R.; Friedman,H.; Sampaio,R. N. R.
Laboratory animals are fundamental to study immunological aspects and the efficacy of new drugs to treat leishmaniasis. However, we do not have practical and good animal models to study leishmaniasis caused by Leishmania (Viannia) braziliensis - L(V)b. In this study, thirty-two experimental animals (Cavia porcellus) were injected in the hind foot with 3x10(5) promastigote forms of L(V)b. The animals were followed for eight weeks. None of the experimental animals developed lesions or presented the parasite in any of the tests performed (histopathological exam, smears, culture, inoculation in hamsters, and polymerase chain reaction).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental leishmaniasis; Cavia; Animal model; American leishmaniasis; Pathogenesis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400017

Aggravation of pathogenesis mediated by aflatoxin B1 in mice infected with Trypanosoma brucei rhodesiense
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Kibugu, J.K; Makumi, J.N; Ngeranwa, J.J.N; Kagira, J.M; Gathumbi, J.K; Mwangi, J.N.
Aflatoxins are known to alter the pathogenesis of many infectious diseases, but such effects have not been evaluated in trypanosome infections. The aim of the present work was to assess the effects of aflatoxin B1 on the pathogenesis of Trypanosoma brucei rhodesiense infection using a murine model. Mice fed on 0.50 mg/kg aflatoxin b. wt. were infected with T. b. rhodesiense and compared to trypanosome infected and uninfected aflatoxin-fed controls. The clinical and pathological changes were determined and the quantitative data statistically analysed using standard methods. The results showed that infected aflatoxin-fed mice had pronounced dyspnoea, significantly (P&lt;0.05) reduced survival, extreme emaciation, pronounced macrocytic normochromic anaemia...
Palavras-chave: Aflatoxin B1; Trypanosomiasis; Pathogenesis; Mice.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2384

DNA Banding Patterns of Trichomonas vaginalis Strains Isolated from Symptomatic and Asymptomatic Subjects
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Sapru, Kusum; Mohan, K.; Gupta, Indu; Ganguly, N. K.; Mahajan, R. C.; Malla, Nancy.
Palavras-chave: Trichomonas vaginalis; Trichomoniasis; DNA banding pattern; Pathogenesis.
Ano: 1994
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/203

Host-parasite relationship during Epistylis sp. (Ciliophora: Epistylididae) infestation in farmed cichlid and pimelodid fish
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Pádua,Santiago Benites de; Martins,Maurício Laterça; Valladão,Gustavo Moraes Ramos; Utz,Laura; Zara,Fernando José; Ishikawa,Márcia Mayumi; Belo,Marco Antonio de Andrade.
Abstract: The objective of this work was to describe the host-Epistylis sp. relationship during infestation on farmed fish. Five Nile tilapia (Oreochromis niloticus) and ten hybrid surubim catfish (Pseudoplatystoma reticulatum x P. corruscans), all diseased, were used for in vivo morphological analysis of sessile peritrichs by contrast microscopy. Fragments of infected tissues were subjected to histological processing and scanning electron microscopy. Epistylis sp. caused hemorrhagic ulcer disease, and cichlids were more prone to develop infestations throughout the body surface due to the attachment of the colonies to the scales, which did not occur with pimelodids. Multifocal granulomatous dermatitis was observed, associated with the hydropic degeneration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oreochromis niloticus; Pseudoplatystoma reticulatum; Pseudoplatystoma corruscans; Histopathology; Pathogenesis; Sessile peritrich.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500520

Nitric oxide in plants: a brief discussion on this multifunctional molecule
Provedor de dados: Scientia Agricola
Autores: Ferreira,Leonardo Cesar; Cataneo,Ana Catarina.
Several studies were carried out in order to improve the knowledge about the occurrence and activity of nitric oxide (NO) in plants. Thus, this review discusses some aspects related to NO in plants such as chemical properties, synthesis pathways, physiological effects, antioxidant action, signal transduction, interaction with plant hormones and gene expression. In the last years, many advances have been obtained regarding NO synthesis and its physiological effects in plants. However, the molecular mechanisms underlying its effects remain poorly understood. It is signalized that tight interplays among NO, Ca2+, cyclic ADP ribose (cADPR), and protein kinases need to be investigated in details. In addition, it has not yet been possible to identify a plant...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Pathogenesis; Plant hormones; Plant signal transduction; Reactive oxygen species.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000200017

Clinical and molecular aspects of severe malaria
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: Kirchgatter,Karin; Del Portillo,Hernando A.
The erythrocytic cycle of Plasmodium falciparum presents a particularity in relation to other Plasmodium species that infect man. Mature trophozoites and schizonts are sequestered from the peripheral circulation due to adhesion of infected erythrocytes to host endothelial cells. Modifications in the surface of infected erythrocytes, termed knobs, seem to facilitate adhesion to endothelium and other erythrocytes. Adhesion provides better maturation in the microaerophilic venous atmosphere and allows the parasite to escape clearance by the spleen which recognizes the erythrocytes loss of deformability. Adhesion to the endothelium, or cytoadherence, has an important role in the pathogenicity of the disease, causing occlusion of small vessels and contributing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Severe malaria; Plasmodium falciparum; PfEMP1; Pathogenesis; Cytoadherence; Rosetting; Antigenic variation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000300008

Fluorescent antibody test, quantitative polymerase chain reaction pattern and clinical aspects of rabies virus strains isolated from main reservoirs in Brazil
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Appolinário,Camila; Allendorf,Susan Dora; Vicente,Acácia Ferreira; Ribeiro,Bruna Devidé; Fonseca,Clóvis Reinaldo da; Antunes,João Marcelo; Peres,Marina Gea; Kotait,Ivanete; Carrieri,Maria Luiza; Megid,Jane.
ABSTRACTRabies virus (RABV) isolated from different mammals seems to have unique characteristics that influence the outcome of infection. RABV circulates in nature and is maintained by reservoirs that are responsible for the persistence of the disease for almost 4000 years. Considering the different pattern of pathogenicity of RABV strains in naturally and experimentally infected animals, the aim of this study was to analyze the characteristics of RABV variants isolated from the main Brazilian reservoirs, being related to a dog (variant 2),Desmodus rotundus (variant 3), crab eating fox, marmoset, and Myotis spp. Viral replication in brain tissue of experimentally infected mouse was evaluated by two laboratory techniques and the results were compared to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pathogenesis; QRT-PCR; FAT; Rabies; Variants.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000500479

Japanese encephalitis: a review of the Indian perspective
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Tiwari,Sarika; Singh,Rishi Kumar; Tiwari,Ruchi; Dhole,Tapan N.
Japanese encephalitis virus (JEV) causes Japanese encephalitis, which is a leading form of viral encephalitis in Asia, with around 50,000 cases and 10,000 deaths per year in children below 15 years of age. The JEV has shown a tendency to extend to other geographic regions. Case fatality averages 30% and a high percentage of the survivors are left with permanent neuropsychiatric sequelae. Currently, there is no cure for JEV, and treatment is mainly supportive. Patients are not infectious, but should avoid further mosquito bites. A number of antiviral agents have been investigated; however, none of these have convincingly been shown to improve the outcome of JEV. In this review, the current knowledge of the epidemiology and the pathogenesis of this deadly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epidemiology; Pathogenesis; Vector born diseases; Epidemics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600011

Genotypic study documents divergence in the pathogenesis of bloodstream infection related central venous catheters in neonates
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Brito,Cristiane Silveira; Ribas,Rosineide Marques; Resende,Daiane Silva; Brito,Denise Von Dolinger de; Abdallah,Vânia Olivetti Steffen; Santos,Kátia Regina Netto dos; Cavalcante,Fernanda Sampaio; Matos,Pricilla Dias Moura de; Gontijo Filho,Paulo P.
OBJECTIVE: To investigate the pathogenesis of bloodstream infection by Staphylococcus epidermidis, using the molecular epidemiology, in high-risk neonates. METHODS: We conducted a prospective study of a cohort of neonates with bloodstream infection using central venous catheters for more than 24 h. "National Healthcare Safety Network" surveillance was conducted. Genotyping was performed by DNA fingerprinting and mecA genes and icaAD were detected by multiplex-PCR. RESULTS: From April 2006 to April 2008, the incidence of bloodstream infection and central venous catheter-associated bloodstream infection was 15.1 and 13.0/1000 catheter days, respectively, with S. epidermidis accounting for 42.9% of episodes. Molecular analysis was used to document the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neonates; Central venous catheter; Pathogenesis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000400387

GBV-C/HGV and HIV-1 coinfection
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Maidana,Maria Teresa; Sabino,Ester Cerdeira; Kallas,Esper Georges.
An interesting interaction pattern has been found between HIV-1 and GBV-C/HGV, resulting in protection against progression to AIDS. The mechanisms involved in this interaction remain to be clarified. We examined the current knowledge concerning this coinfection and developed hypotheses to explain its effects. A better understanding of this interaction could result in new concepts, which may lead to new strategies to control HIV-1 replication and progression to AIDS.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; HGV; GBV-C; Pathogenesis; Review.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000200001

Study of biological characteristics of Pseudomonas aeruginosa strains isolated from patients with cystic fibrosis and from patients with extra-pulmonary infections
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Stehling,Eliana Guedes; Silveira,Wanderley Dias da; Leite,Domingos da Silva.
A total of 120 strains of Pseudomonas aeruginosa, isolated from cystic fibrosis (CF) patients (n = 80) and from patients having extra-pulmonary infections (n = 40) were studied regarding the presence of some virulence factors (hemolysin, gelatinase and elastase production) and presence of the algD and algU genes as detected by polymerase chain reaction-PCR. There was not a significant difference for the production of gelatinase and hemolysin between non-mucoid strains from CF patients and other isolates from extra-pulmonary infections and mucoid strains. The production of elastase was found to be significant among these strains. The algD gene was detected by PCR in all studied strains but the algU gene was detected only in 25% of the mucoid strains....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas aeruginosa; Biological characterization; Pathogenesis; Cystic fibrosis; Extra-pulmonary infections.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100018

Cytokine expression patterns and mesenchymal stem cell karyotypes from the bone marrow microenvironment of patients with myelodysplastic syndromes
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Xiong,H.; Yang,X.Y.; Han,J.; Wang,Q.; Zou,Z.L.
The purpose of this study was to explore cytokine expression patterns and cytogenetic abnormalities of mesenchymal stem cells (MSCs) from the bone marrow microenvironment of Chinese patients with myelodysplastic syndromes (MDS). Bone marrow samples were obtained from 30 cases of MDS (MDS group) and 30 healthy donors (control group). The expression pattern of cytokines was detected by customized protein array. The karyotypes of MSCs were analyzed using fluorescence in situ hybridization. Compared with the control group, leukemia inhibitory factor, stem cell factor (SCF), stromal cell-derived factor (SDF-1), bone morphogenetic protein 4, hematopoietic stem cell (HSC) stimulating factor, and transforming growth factor-β in the MDS group were significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow microenvironment; Cytokine; Mesenchymal stem cell; Chromosome; Pathogenesis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300207

The role of necroptosis in neurosurgical diseases
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Liu,T.; Bao,Y.H.; Wang,Y.; Jiang,J.Y.
Programmed necrosis or necroptosis is an alternative form of cell death that is executed through a caspase-independent pathway. Necroptosis has been implicated in many pathological conditions. Genetic or pharmacological inhibition of necroptotic signaling has been shown to confer neuroprotection after traumatic and ischemic brain injury. Therefore, the necroptotic pathway represents a potential target for neurological diseases that are managed by neurosurgeons. In this review, we summarize recent advances in the understanding of necroptotic signaling pathways and explore the role of necroptotic cell death in craniocerebral trauma, brain tumors, and cerebrovascular diseases.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular mechanism; Pathogenesis; Programmed cell death; Therapy.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400292

A bovine herpesvirus 5 recombinant defective in the thymidine kinase (TK) gene and a double mutant lacking TK and the glycoprotein E gene are fully attenuated for rabbits
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Silva,S.C.; Brum,M.C.S.; Weiblen,R.; Flores,E.F.; Chowdhury,S.I.
Bovine herpesvirus 5 (BoHV-5), the agent of herpetic meningoencephalitis in cattle, is an important pathogen of cattle in South America and several efforts have been made to produce safer and more effective vaccines. In the present study, we investigated in rabbits the virulence of three recombinant viruses constructed from a neurovirulent Brazilian BoHV-5 strain (SV507/99). The recombinants are defective in glycoprotein E (BoHV-5gEΔ), thymidine kinase (BoHV-5TKΔ) and both proteins (BoHV-5gEΔTKΔ). Rabbits inoculated with the parental virus (N = 8) developed neurological disease and died or were euthanized in extremis between days 7 and 13 post-infection (pi). Infectivity was detected in several areas of their brains. Three of 8 rabbits inoculated with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BoHV-5; GE and TK deletion mutants; Pathogenesis; Virulence; Vaccine candidate.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200005

Filaggrin silencing by shRNA directly impairs the skin barrier function of normal human epidermal keratinocytes and then induces an immune response
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Dang,N.N.; Pang,S.G.; Song,H.Y.; An,L.G.; Ma,X.L.
The objective of this study was to investigate whether a single defect in skin barrier function simulated by filaggrin silencing could induce Th2-predominant inflammation. Filaggrin gene expression was silenced in cultured normal human epidermal keratinocytes (NHEKs) using small hairpin RNA (shRNA, GTTGGCTCAAGCATATTATTT). The efficacy of silencing was confirmed by polymerase chain reaction (PCR) and Western blotting. Filaggrin-silenced cells (LV group), shRNA control cells (NC group), and noninfected cells (Blank group) were evaluated. The expression of cornified cell envelope-related proteins, including cytokeratin (CK)-5, -10, -14, loricrin, involucrin, and transglutaminase (TGM)-1, was detected by Western blotting. Interleukins (IL)-2, IL-4, IL-5,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atopic dermatitis; T helper 2 cytokines; Filaggrin silencing; Pathogenesis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100039

Gene expression profiling analysis of lung adenocarcinoma
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Xu,H.; Ma,J.; Wu,J.; Chen,L.; Sun,F.; Qu,C.; Zheng,D.; Xu,S.
The present study screened potential genes related to lung adenocarcinoma, with the aim of further understanding disease pathogenesis. The GSE2514 dataset including 20 lung adenocarcinoma and 19 adjacent normal tissue samples from 10 patients with lung adenocarcinoma aged 45-73 years was downloaded from Gene Expression Omnibus. Differentially expressed genes (DEGs) between the two groups were screened using the t-test. Potential gene functions were predicted using functional and pathway enrichment analysis, and protein-protein interaction (PPI) networks obtained from the STRING database were constructed with Cytoscape. Module analysis of PPI networks was performed through MCODE in Cytoscape. In total, 535 upregulated and 465 downregulated DEGs were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung adenocarcinoma; Pathogenesis; Differentially expressed genes; Protein-protein interaction; Network module.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300601

Virulence of oral Fusobacterium nucleatum from humans and non-human primates in mice
Provedor de dados: Brazilian Journal of Microbiology
Autores: Gaetti-Jardim Júnior,Elerson; Luvizotto,Maria C. Rui; Avila-Campos,Mario J.
The lethal action in Balb/c mice of 80 oral Fusobacterium nucleatum recovered from patients with adult periodontitis, healthy subjects or Cebus apella monkeys was studied. Mice were inoculated intraperitoneally with each bacterial inoculum of 5 x 10(8) CFU/ml. All the clinical isolates induced weight and coordinated movements loss. Pathological alterations in liver, CNS, heart, and kidney with inflammatory reactions or vascular congestion were observed. Of all the tested F. nucleatum isolates, 61.2% from periodontal patients, 57.1% from healthy subjects and 60% from monkeys, were capable of killing the mice in 48h. The clinical isolates were significantly more pathogenic than F. nucleatum ATCC 10953 or ATCC 25586. B. fragilis ATCC 23745 showed lethality...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fusobacterium nucleatum; Pathogenesis; Human and non-human primates.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200016

An in-house multiplex pcr method to detect of putative virulence factors in Aeromonas species
Provedor de dados: Brazilian Journal of Microbiology
Autores: Aguilera-Arreola,Ma. Guadalupe; Martínez,Alma Aidee Carmona; Castro-Escarpulli,Graciela.
A pentaplex PCR was developed and optimised to detect the genes that encode the five most important putative virulence factors in Aeromonas isolates. It seems to be more efficient than previously reported techniques and promises to be a powerful tool for more accurate risk assessments and for monitoring pathogenic strains.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pentaplex-PCR; Virulence potential; Pathogenesis; Aeromonas.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400011

Porcine circovirus 2 (PCV2) increases the expression of endothelial adhesion/junction molecules
Provedor de dados: Brazilian Journal of Microbiology
Autores: Marks,Fernanda S.; Almeida,Laura L.; Driemeier,David; Canal,Cláudio; Barcellos,David E.S.N.; Guimarães,Jorge A.; Reck,José.
Abstract Porcine circovirus type 2 (PCV2) is the primary causative agent of porcine circovirus disease, a complex multisystem syndrome in domestic pigs. Despite the significant economic losses caused by porcine circovirus disease, the mechanisms of pathogenesis underlying the clinical findings remain largely unclear. As various reports have highlighted the potential key role of vascular lesions in the pathogenesis of porcine circovirus disease, the aim of this work was to investigate effects of PCV2 infection on vascular endothelial cells, focusing on cell viability and expression of adhesion/junction molecules. PCV2 infection reduced endothelial cell viability, while viral infection did not affected the viability of several other classical cell lines....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swine; Infectious diseases; Pathogenesis; Viral infection; Cell adhesion.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400870