Consulta: subjectFacets:"Pectinases"
Registros recuperados: 12
Data/hora: 12/06/2024 16:17:40

Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectin
Provedor de dados: Electronic Journal of Biotechnology
Autores: Rojas,Natalia Lorena; Cavalitto,Sebastián Fernando; Mignone,Carlos Fernando; Hours,Roque Alberto.
Protopectinases (PPases) constitute a heterogeneous group of extracellular enzymes able to release soluble pectin from insoluble protopectin in plant tissues. Geotrichum klebahnii (ATCC 42397) produces PPase-SE with endopolygalacturonase activity. PPase-SE has been used for pectin extraction and maceration of plant tissues. Here, the capacity of G. klebahnii to use different pectins as carbon and energy sources (CES) was studied, in addition to PPase-SE capacity to release pectin from lemon peel. The strain was unable to use pectin from different origins as CES. When G. klebahnii was cultivated with mixtures of different amounts of glucose and citrus pectin as CES, the biomass obtained was proportional to the initial concentration of glucose, which was...
Tipo: Journal article
Palavras-chave: Carbon balance; Enzymatic pectin extraction; Microbial pectin utilization; Pectinases; Synthetic medium.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100012

Kinetic study on inducibility of polygalacturonases from Aspergillus flavipes FP-500
Provedor de dados: Electronic Journal of Biotechnology
Autores: Martínez-Trujillo,Aurora; Aranda,Juan S; Aguilar-Osorio,Guillermo.
The aim of this work was to describe growth dynamics, substrate depletion and polygalacturonases production by Aspergillus flavipes FP-500 in batch cultures by means of unstructured models. The microorganism was cultivated on several mono- di- and poly- saccharides, and then the culture development modeled with Monod and Leudeking-Piret equations. The kinetic parameters related to the models (µmax, &amp;#947;x/s, &amp;#945; and &amp;#946;) were obtained by minimizing the quadratic residuals function with a simplex algorithm. An accurate description of experimental data was attained with the proposed models. Besides, modeling provided significant kinetic information on microbial degradation of complex substrates, such as the correlation between specific...
Tipo: Journal article
Palavras-chave: Constitutive enzymes; Inducible enzymes; Initial pH; Kinetic modeling; Pectinases.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400008

Efeito dos complexos enzimáticos clarificantes Clarex e CEC1-CTAA sobre a qualidade do suco de banana
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Cardoso,Marisa Helena; Menezes,Hilary Castle de; Jackix,Marisa de Nazaré Hoelz; Gonçalves,Elisabeth Borges.
Neste trabalho foi observado o efeito dos complexos enzimáticos clarificantes Clarex e CEC1-CTAA, adicionados na proporção de 0,03% v/p sobre purê de banana (Musa cavendishii), em condições amenas de hidrólise (40ºC, 15 minutos) visando determinar a qualidade, aqui representada pelos indicadores: rendimento; viscosidade; Brix; pH; composição centesimal; contagens de bolores e leveduras e de mesófilos, e propriedades sensoriais de cor, aroma, sabor e corpo dos sucos de banana clarificados. O suco clarificado com Clarex apresentou-se significativamente (p &lt; 0,01) mais amarelo, menos cinza, menos opaco e menos viscoso do que o obtido com CEC1-CTAA. Não houve diferença significativa entre as médias de aroma de banana fresca e sabor nesses sucos. Além disso,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pectinases; Processamento do suco.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X1999000500016

Caracterização da poligalacturonase produzida por fermentação semi-sólida utilizando-se resíduo do maracujá como substrato
Provedor de dados: Revista Brasileira de Engenharia Agrícola e Ambiental
Autores: Souza,Rosane L. A. de; Oliveira,Líbia de S. C.; Silva,Flávio L. H. da; Amorim,Beatriz C.
As enzimas pectinolíticas ou pectinases são um grupo heterogêneo de enzimas que hidrolisam as substâncias pécticas presentes nas células vegetais. Essas enzimas estão sendo aplicadas em diversas áreas tornando-se importante conhecer suas características visando uma aplicação eficiente. Assim, este trabalho, objetivou-se caracterizar o resíduo seco da casca e do albedo do maracujá (Passiflora edulis) e posterior caracterização da enzima poligalacturonase, produzida através da fermentação em estado sólido e, como agente da fermentação, o fungo filamentoso Aspergillus niger. O resíduo do maracujá apresenta teor de pectina de 13,10%. A atividade de poligalacturonase alcançou um pico em 66 h de processo, para 40% de umidade inicial e 1% da concentração da fonte...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passiflora edulis; Pectinases; Aspergillus niger; Pectina.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662010000900011

Sugar-cane juice induces pectin lyase and polygalacturonase in Penicillium griseoroseum
Provedor de dados: Revista de Microbiologia
Autores: Minussi,Rosana Cristina; Soares-Ramos,Juliana Rocha Lopes; Coelho,Jorge Luiz Cavalcante; Silva,Daison Olzany.
The use of other inducers as substitutes for pectin was studied aiming to reduce the production costs of pectic enzymes. The effects of sugar-cane juice on the production of pectin lyase (PL) and polygalacturonase (PG) by Penicillium griseoroseum were investigated. The fungus was cultured in a mineral medium (pH 6.3) in a rotary shaker (150 rpm) for 48 h at 25oC. Culture media were supplemented with yeast extract and sucrose or sugar-cane juice. Sugar-cane juice added singly to the medium promoted higher PL activity and mycelial dry weight when compared to pectin and the use of sugar-cane juice and yeast extract yielded levels of PG activity that were similar to those obtained with sucrose-yeast extract or pectin. The results indicated that, even at low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pectin lyase; Polygalacturonase; Pectinases; Penicillium griseoroseum; Sugar-cane juice.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400002

Isolamento e regeneração de protoplastos de Penicillium brevicompactum - DOI: 10.4025/actascibiolsci.v26i4.1529
Provedor de dados: Acta Scientiarum: Biological Sciences
Autores: Varavalho, Maurílio Antônio; Centro Universitario das Faculdades Metropolitanas Unidas; Queiroz, Marisa Vieira de; UFV; Pereira, Jorge Fernando; UFV; Araújo, Elza Fernandes de; UFV.
O isolamento e regeneração de protoplastos de fungos é um passo fundamental para o estabelecimento de sistemas de transformação, análise do cariótipo  molecular e fusão entre linhagens, que são técnicas de ampla aplicação em programas de melhoramento para fungos filamentosos. Neste trabalho foram testados diferentes preparações enzimáticas e estabilizadores osmóticos para estabelecer condições otimizadas de isolamento e regeneração de protoplastos de Penicillium brevicompactum, que é um excelente produtor de pectinases. Protoplastos de P. brevicompactum foram obtidos em maior quantidade quando o micélio foi digerido com 15mg.mL-1 de Glucanex (Novo Nordisk) em NaCl 0,8 mol.L-1 como estabilizador osmótico. O melhor estabilizador osmótico para a regeneração...
Palavras-chave: 2.01.00.00-0    Biologia Geral Penicillium; Pectinases; Protoplastos 2.01.00.00-0    Biologia Geral.
Ano: 2004
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1529

Atividade pectinolítica de Colletotrichum gloeosporioides e a relação com a agressividade ao Stylosanthes spp
Provedor de dados: Bragantia
Autores: Marchi,Carlos Eduardo; Fernandes,Celso Dornelas; Guimarães,Luciana Roberta de Araújo; Fabris,Larissa Rodrigues; Borges,Mirian de Freitas; Trentin,Renata Aline; Jerba,Vanessa de Fátima.
A produção de pectinases de 40 isolados de Colletotrichum gloeosporioides, obtidos de Stylosanthes spp. em diferentes regiões produtoras, foi estimada por meio de difusão enzimática em meio específico e mensuração do halo de degradação de pectina. Em todos os isolados havia atividade pectinolítica, sendo divididos em cinco grupos distintos. Na maior parte dos isolados notou-se moderada atividade de pectinases. Em geral, isolados de Camapuã-MS tiveram menor diversidade genética para a produção de enzimas pécticas. Com base na atividade pectinolítica, isolados do patógeno foram selecionados e inoculados em genótipos de S. capitata (GC1081, GC1084, GC1086 e GC1094), S. scabra (GC1496) e S. macrocephala (GC1511), visando analisar a influência das pectinases na...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pectinases; Enzimas degradadoras de parede celular; Glomerella cingulata; Antracnose; Variabilidade patogênica; Estilosantes.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052009000200017

Extracellular enzymes of Fusarium graminearum isolates
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Kikot,Gisele Eleonora; Hours,Roque Alberto; Alconada,Teresa María.
Fusarium graminearum isolates from three different agroecological regions in Argentina were examined according to the production of different extracellular enzyme activities of potential biotechnological interest: pectinases (PGase: polygalacturonase and PMGase: polymethylgalacturonase), cellulase (CMCase: carboxymethylcellulase) and hemicellulase (xylanase). The isolates were grown in minimum salt medium supplemented with 0.25% glucose, 0.125% citric pectin and 0.125% oat bran as carbon sources and/or enzyme inducers. PGase activity was detected early (after two days of incubation) in all the cultures; it was found to be the highest for all the isolates. PMGase was high only for those isolates of the II region. CMCase and endoxylanase activities were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular enzymes; Fusarium graminearum; Pectinases; Cellulases; Hemicellulases.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400005

Production of pectinases by A. niger: influence of fermentation conditions
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Martos,María A.; Martinez Vazquez,Francisco; Benassi,Fernando O.; Hours,Roque A.
Response surface methodology was used for optimization of polygalacturonase (PG) and pectinesterase (PE) production in submerged fermentation by A.niger. A Central Composite Experimental Design was applied, consisting of 22 experiments, including eight central points. Variables studied were: fermentation time (24 to 120 h), pH (3.5 to 6.5) and initial concentration of pectin (5 to 20 g/l). Maximum PE production was 220 U/l, after 74 h of culture, in a medium containing 20 g/l of pectin (pH 6.5). The optimal conditions for PG production were pH: 4.1, 20 g/l of pectin and 94 h of fermentation with a maximum value of 1032 U/l. Under these conditions, the PE production was low (15 U/l). A liquid extract with high PG activity and low PE activity could be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pectinases; Polygalacturonase; Pectinesterase; Aspergillus niger; Response surface methodology; Citrus pectin.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300007

Carbon sources effect on pectinase production from Aspergillus japonicus 586
Provedor de dados: Brazilian Journal of Microbiology
Autores: Teixeira,Maria F. S.; Lima Filho,José L.; Durán,Nelson.
The effect of different carbon sources on the pectinesterases, endo- and exo-polygalacturonase activities from Aspergillus japonicus 586 was evaluated in liquid media (Manachini solutions) supplemented with different substrate concentrations. The culture medium was inoculated with 5.10(6) spores/ml and mantained under agitation (140 rpm), at 30°C, during 122 h. The enzyme evaluation was carried out 24 h after filtration. The crude extract from A. japonicus 586 indicated that the best enzymatic activities were afforded in the presence of 0.5% pectin (pectinesterease), 0.2% pectin and 0.2% glycerol (endopolygalacturonase), and 0.5% pectin associated to 0.5% glucose (exopolygalacturonase). Carbon sources concentration, isolated or associated, significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pectinases; Aspergillus japonicus; Enzyme; Galacturonase; Pectinesterases.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400009

Production of pectin lyase by Penicillium griseoroseum in bioreactors in the absence of inducer
Provedor de dados: Brazilian Journal of Microbiology
Autores: Piccoli-Valle,Roberta Hilsdorf; Passos,Flávia Maria Lopes; Passos,Frederico José Vieira; Silva,Daison Olzany.
Penicillium griseoroseum was grown in bioreactors on mineral medium supplemented with yeast extract and sucrose. The influence of inoculum and carbon source concentrations, aeration and pH on pectin lyase (PL) production, as well as the capacity of P. griseoroseum to produce PL when grown on sugar cane syrup as carbon source were evaluated. Inoculum concentration did not influence PL production. Production was higher in non-aerated than in aerated medium. The best results were obtained using 60 mM sucrose at pH 6.3-7.2. Production using cane syrup 25% (v/v), without yeast extract supplement, was equal to that obtained under the conditions cited above.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fermentation; Pectin lyase; Penicillium griseoroseum; Pectinases.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200013

Isolation of recombinant strains with enhanced pectinase production by protoplast fusion between Penicillium expansum and Penicillium griseoroseum
Provedor de dados: Brazilian Journal of Microbiology
Autores: Varavallo,Maurilio Antonio; Queiroz,Marisa Vieira de; Lana,Taís Guimarães; Brito,Admilson Toscano Ribeiro de; Gonçalves,Daniel Bonoto; Araújo,Elza Fernandes de.
Protoplast fusion between complementary auxotrophic and morphological mutant strains of Penicillium griseoroseum and P. expansum was induced by polyethylene glycol and calcium ions (Ca2+). Fusant strains were obtained in minimal medium and a prototrophic strain, possibly diploid, was chosen for haplodization with the fungicide benomyl. Different recombinant strains were isolated and characterized for occurrence of auxotrophic mutations and pectinolytic enzyme production. The fusant prototrophic did not present higher pectinase production than the parental strains, but among 29 recombinants analyzed, four presented enhanced enzyme activities. The recombinant RGE27, which possesses the same auxotrophic and morphologic mutations as the P. griseoroseum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protoplast fusion; Pectinases; Penicillium.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100011