Consulta: subjectFacets:"Peptides"
Registros recuperados: 31
Data/hora: 04/06/2024 02:19:29

Peptide synthesis: chemical or enzymatic
Provedor de dados: 69
Autores: Guzmán,Fanny; Barberis,Sonia; Illanes,Andrés.
Peptides are molecules of paramount importance in the fields of health care and nutrition. Several technologies for their production are now available, among which chemical and enzymatic synthesis are especially relevant. The present review pretends to establish a non-biased appreciation of the advantages, potentials, drawbacks and limitations of both technologies. Chemical synthesis is thoroughly reviewed and their potentials and limitations assessed, focusing on the different strategies and challenges for large-scale synthesis. Then, the enzymatic synthesis of peptides with proteolytic enzymes is reviewed considering medium, biocatalyst and substrate engineering, and recent advances and challenges in the field are analyzed. Even though chemical synthesis...
Tipo: Journal article
Palavras-chave: Enzymatic synthesis; Peptides; Proteases; Solid-phase synthesis.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200012

Nova Estratégia Metodológica para Análise Eletroforética (Tricina-SDS-PAGE), visando à Identificação Peptídeo-proteica em Soja.
Provedor de dados: 14
Autores: STEPHAN, M. P.; FELBERG, I.; SANTOS, A. A. dos; AZEVEDO, T. de L.; CARRÃO-PANIZZI, M. C.
Recentemente, tem sido mostrado o potencial medicinal de peptídeos bioativos oriundos tanto da soja amarela quanto da preta. Publicações sobre a caracterização molecular de hidrolisados proteicos de soja amarela por eletroforese em gel de poliacrilamida (SDS-PAGE) não mostram a visualização de peptídeos na faixa de 10 kDa e 1 kDa. Nesse trabalho, desenvolveu-se uma nova estratégia metodológica de preparo da amostra, junto com a utilização das eletroforeses dos tipos SDS-PAGE e TRIS/TRICINA. Os extratos parcialmente purificados mostraram a obtenção de um gel límpido contendo cadeias peptídicas-proteicas bem separadas. Como destaque foi evidenciada uma proteína com massa acima de 100 kDa e um peptídeo de 11 kDa, somente para a soja preta. Esta técnica de...
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Caracterização molecular; Extrato de soja; Eletroforese; Proteína; Peptídeo; Tecnologia de Alimento; Food technology; Proteins; Peptides; Soy protein.
Ano: 2019
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1117697

The effects of dietary marine protein hydrolysates on the development of sea bass larvae, Dicentrarchus labrax, and associated microbiota
Provedor de dados: 5
Autores: Delcroix, Jeremy; Gatesoupe, F.j.; Desbruyeres, Elisabeth; Huelvan, Christine; Le Delliou, Herve; Le Gall, Marie-madeleine; Quazuguel, Patrick; Mazurais, David; Zambonino-infante, Jose-luis.
Protein hydrolysate is an essential component of dry starter diets for fish larvae, as promoting healthy development. Peptides are also suitable substrates for many intestinal microbes. Five experimental diets were compared to a control diet (CONT) supplemented with a commercial fish protein hydrolysate. Each diet contained one marine protein hydrolysate, which differed by the proportion of di- and tri-peptides, and by raw materials. Two diets (HYD4 and HYD5) stimulated larval growth compared with CONT. Two other diets (HYD1 and HYD2) yielded inferior growth. HYD1 was detrimental for survival, which was associated with an up-regulation of genes involved in inflammation and antioxidative responses, while the gene coding for osteocalcin was down-regulated in...
Tipo: Text
Palavras-chave: Compound diets; Denaturing gradient gel electrophoresis; Digestive enzymes; Gene expression; Gut maturation; Peptides.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00197/30865/29268.pdf

Valorisation de matières premières marines de faible valeur ajoutée : application aux co-produits de thon
Provedor de dados: 5
Autores: Nguyen, Thi-my-huong.
The main objective of this study is the valorisation of by-products coming from processed tuna (Thunnus albacares). Therefore, enzymatic hydrolysis was selected, using an industrial large spectrum protease (Protamex). In a first phase, hydrolyses of these by-products (heads, tails, viscera) were carried out for long periods of time (12h) in order to establish the kinetics of the proteolysis of those different by-products and to obtain the approximate composition of the different resulting fractions and to determine the conditions which would allow the  valorisation of these  fractions.  In a second phase the valorisation in animal nutrition (shrimp culture) and in human nutrition (fish sauce) of the fractions coming from the hydrolysis of the head of tunas...
Tipo: Text
Palavras-chave: Feed; Food; Lipids; Peptids; Protamex; Proteolysis; By products; Thuna; Alimentation; Lipides; Peptides; Protamex; Protéolyse; Co produits; Thon.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/these-6918.pdf

Evolution of the Toxoglossa Venom Apparatus as Inferred by Molecular Phylogeny of the Terebridae
Provedor de dados: 5
Autores: Holford, Mande; Puillandre, Nicolas; Terryn, Yves; Cruaud, Corinne; Olivera, Baldomero; Bouchet, Philippe.
Toxoglossate marine gastropods, traditionally assigned to the families Conidae, Terebridae, and Turridae, are one of the most populous animal groups that use venom to capture their prey. These marine animals are generally characterized by a venom apparatus that consists of a muscular venom bulb and a tubular venom gland. The toxoglossan radula, often compared with a hypodermic needle for its use as a conduit to inject toxins into prey, is considered a major anatomical breakthrough that assisted in the successful initial radiation of these animals in the Cretaceous and early Tertiary. The pharmacological success of toxins from cone snails has made this group a star among biochemists and neuroscientists, but very little is known about toxins from the other...
Tipo: Text
Palavras-chave: Life history similarities; Similar proboscis type; Morphology; Biology; Peptides; Radula.
Ano: 2009
URL: https://archimer.ifremer.fr/doc/00207/31788/30197.pdf

Implication des peptides antimicrobiens dans l'immunité innée de la moule, Mytilus galloprovincialis
Provedor de dados: 5
Autores: Mitta, Guillaume.
Les mécanismes immunitaires innés des vertébrés sont une mosaïque de mécanismes ancestraux partagés avec des animaux évolutivement plus primitifs. Les peptides antimicrobiens sont une de ces composantes conservées au travers du règne animal. En effet, ils participent vraisemblablement chez tous les métazoaires aux processi d'immunité innée. Toutefois, leurs implications dans la réponse anti-infectieuse peuvent être assez différentes. L'objet de cette thèse était de comprendre comment ces peptides participaient aux processi de défense de la moule. Une première phase de caractérisation biochimique de telles molécules avait été entreprise chez la moule, Mytilus galloprovincialis, dans le cadre de la thèse de Florence Hubert. Notre travail a consisté (i) à...
Tipo: Text
Palavras-chave: Immunité; Peptides; Antibiotiques; Moule; Défensine.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/00439/55101/56547.pdf

Nutritional components affecting skeletal development in fish larvae
Provedor de dados: 5
Autores: Cahu, Chantal; Zambonino, Jose-luis; Takeuchi, Toshio.
Marine fish larvae undergo major functional and morphological changes during the developmental stages and several factors can interfere with the normal development of larvae and affect fry quality. Skeletal malformations, such as spinal malformation-scoliosis, lordosis, coiled vertebral column-, missing or additional fin rays, bending opercle or jaw malformations, are frequently observed in hatchery-reared larvae. This paper reviews the effects of some nutritional components on skeletal development in larvae of a number of fish species. In the dietary lipid fraction, for instance, it was proven that the phospholipid concentration affected the spinal malformation rate in sea bass fed a compound diet from mouth opening onwards. Phosphatidylinositol, in...
Tipo: Text
Palavras-chave: Retinoic acid; Ascorbic acid; Amino acid; Phospholipid; Peptides; Skeletal development; Quality; Fish larvae.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-443.pdf

Contrôle de la ponte ovulaire chez l'huître creuse Crassostrea gigas : utilisation du modèle Sepia officinalis pour la caractérisation de peptides régulateurs
Provedor de dados: 5
Autores: Bernay, Benoît.
Despite the fact that the involvement of environmental factors in the physiology of the japanese oyster C. gigas is fully described, regulatory peptides are not known. In this study, we focused on peptidergic control of egg-laying. In order to characterize peptides involved in the regulation of egg-laying in C. gigas, the cuttlefish S. officinalis was used as a model. Firstly, regulatory peptides were investigated in the cuttlefish by peptidomic analysis. This approach led to the identification of eleven new ovarian regulatory peptides. Based on conserved tags, these peptides have been classified into two families: the SepCRPs (Sepia Capsule Releasing Peptides) and the OJPs (Ovarian Jelly Peptides). Released by oocytes into the genital coelom, they are...
Tipo: Text
Palavras-chave: Ostréiculture; Ovaire; Spectrométrie de masse; Peptides; Céphalopodes; Bivalves; Mollusques.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/these-2566.pdf

Ontogenèse des fonctions digestives et besoins nutritionnels chez les larves de poissons marins
Provedor de dados: 5
Autores: Cahu, Chantal; Zambonino, Jose-luis.
The production of fish larvae by hatchery shows an intensive increase for several species, but the specific nutritional requirements of larvae were poorly known until some years. We have conducted several experiments aimed to understand the specificities in larvae digestion, and to formulate a compound diet taking into account the differences compared to juveniles. The chosen species was the European sea bass, Dicentrarchus labrax, and the data were validated on different species. Larvae were fed live preys or experimental compound diet, especially formulated for the experiment. It appeared that pancreatic digestive enzymes, such as trypsin and amylase could be assayed in early ontogenesis, before mouth opening, and increase dramatically during the first...
Tipo: Text
Palavras-chave: Peptides; Phospholipid; Digestive enzymes; Nutrition; Larvae; Dicentrarchus labrax.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3332.pdf

Characterization of insecticidal peptides from venom Australian funnel-web spiders
Provedor de dados: 80
Autores: Vonorax,E. J.; Tyler,M. I.; Atkinson,R. K.; Howden,M. E. H.
Australian funnel-web spiders are relatively large primitive hunting spiders. Male Atrax robustus spiders have been responsible for a number of human deaths. Venom was collected from the species Hadronyche infensa (Hickman) [female], H. formidabilis [male and female], H. versuta [female], and A. robustus (Cambridge) [male] and was fractionated by high performance liquid chromatography. This resulted in the isolation and purification of a homologous series of 7 insecticidal peptides of relatively low molecular mass (approximately 4kDa). The amino acid sequences of these toxins consisted of 36 or 37 amino acids and were named atracotoxins. For the major bioassay of these toxins, we used the cotton bollworm, Helicoverpa armigera (Hubner), due to the great...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Australian funnel-web spiders; Hadronyche and Atrax species; Venom; Insecticidal toxins; Peptides; Helicoverpa armigera.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200005

Antibacterial activity of Rhynocoris marginatus (Fab.) and Catamirus brevipennis (Servile) (Hemiptera: reduviidae) venomS against human pathogens
Provedor de dados: 80
Autores: Sahayaraj,K.; Borgio,J. F.; Muthukumar,S.; Anandh,G. P.
The reduviid predators Rhynocoris marginatus (Fab.) and Catamirus brevipennis (Servile) use their venoms to paralyze their preys. We detected the antibacterial activity of R. marginatus and C. brevipennis venoms against seven Gram-negative and four Gram-positive bacteria by using the disc diffusion method. Rhynocoris marginatus venom exhibited antibacterial activity against four Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Salmonella typhimurium) and one Gram-positive (Streptococcus pyogenes). Catamirus brevipennis venom showed antibacterial activity against six Gram-negative (Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus vulgaris, and Salmonella typhimurium) and three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibacterial activity; Reduviid saliva; Protein content; Peptides.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300011

Initial peptidomic profiling of Brazilian sea urchins: Arbacia lixula, Lytechinus variegatus and Echinometra lucunter
Provedor de dados: 80
Autores: Sciani,Juliana Mozer; Emerenciano,Andrews Krupinski; Silva,José Roberto Machado Cunha da; Pimenta,Daniel Carvalho.
Abstract  Background  Sea urchins can be found throughout the Brazilian coast and are reported to be one of the major causes of marine accidents on the shoreline. Although not lethal, these accidents are reported to be extremely painful. In order to understand the toxinology of the Brazilian urchins, a peptidomic approach was performed aiming to characterize the naturally occurring peptides in both the coelomic fluid and the spine.  Methods  Animals were collected without gender distinction and samples of the coelomic fluid and spines extracted were analyzed by RP-HPLC and mass spectrometry for peptide de novo sequencing.  Results  Several peptides were identified either in the coelomic fluid or the spine extract (except for E. lucunter). The peptide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peptides; Toxins; Sea urchin; Peptidomics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100313

In vitro activity of phospholipase A 2 and of peptides from Crotalus durissus terrificus venom against amastigote and promastigote forms of Leishmania (L.) infantum chagasi
Provedor de dados: 80
Autores: Barros,Gustavo A. C.; Pereira,Andreia V.; Barros,Luciana C.; Lourenço Jr,Airton; Calvi,Sueli A.; Santos,Lucilene D.; Barraviera,Benedito; Ferreira Jr,Rui Seabra.
Abstract  Background  American visceral leishmaniasis is caused by the intracellular parasiteLeishmania (L.) infantum chagasi, and transmitted by the sand fly Lutzomyia longipalpis. Since treatment is based on classical chemotherapeutics with significant side effects, the search for new drugs remains the greatest global challenge. Thus, this in vitro study aimed to evaluate the leishmanicidal effect ofCrotalus durissus terrificus venom fractions on promastigote and amastigote forms of Leishmania (L.) infantum chagasi.  Methods  Phospholipase A 2 (PLA 2 ) and a pool of peptide fraction (&lt;3 kDa) were purified from Crotalusvenom. Furthermore, promastigotes and peritoneal macrophages of mice infected by amastigotes were exposed to serial dilutions of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PLA2; Peptides; Crotalus dutissus terrificus; Venom; Leishmanicidal activity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100351

The Cuban scorpion Rhopalurus junceus (Scorpiones, Buthidae): component variations in venom samples collected in different geographical areas
Provedor de dados: 80
Autores: Rodríguez-Ravelo,Rodolfo; Coronas,Fredy I V; Zamudio,Fernando Z; González-Morales,Lidia; López,Georgina Espinosa; Urquiola,Ariel Ruiz; Possani,Lourival D.
Backgound The venom of the Cuban scorpion Rhopalurus junceus is poorly study from the point of view of their components at molecular level and the functions associated. The purpose of this article was to conduct a proteomic analysis of venom components from scorpions collected in different geographical areas of the country. Results Venom from the blue scorpion, as it is called, was collected separately from specimens of five distinct Cuban towns (Moa, La Poa, Limonar, El Chote and Farallones) of the Nipe-Sagua-Baracoa mountain massif and fractionated by high performance liquid chromatography (HPLC); the molecular masses of each fraction were ascertained by mass spectrometry analysis. At least 153 different molecular mass components were identified among...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cuba; Hyaluronidase; Peptides; Phospholipase; Proteomics; Rhopalurus junceus; Scorpion.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100309

Hymenoptera venom review focusing on Apis mellifera
Provedor de dados: 80
Autores: Lima,P. R. de; Brochetto-Braga,M. R.
Hymenoptera venoms are complex mixtures containing simple organic molecules, proteins, peptides, and other bioactive elements. Several of these components have been isolated and characterized, and their primary structures determined by biochemical techniques. These compounds are responsible for many toxic or allergic reactions in different organisms, such as local pain, inflammation, itching, irritation, and moderate or severe allergic reactions. The most extensively characterized Hymenoptera venoms are bee venoms, mainly from the Apis genus and also from social wasps and ant species. However, there is little information about other Hymenoptera groups. The Apis venom presents high molecular weight molecules - enzymes with a molecular weight higher than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hymenoptera; Venom; Enzymes; Peptides.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200002

Melittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune response
Provedor de dados: 80
Autores: Pereira,Andreia Vieira; Barros,Gustavo de; Pinto,Erika Gracielle; Tempone,Andre Gustavo; Orsi,Ricardo de Oliveira; Santos,Lucilene Delazari dos; Calvi,Sueli; Ferreira Jr,Rui Seabra; Pimenta,Daniel Carvalho; Barraviera,Benedito.
Abstract  Background Apis mellifera venom, which has already been recommended as an alternative anti-inflammatory treatment, may be also considered an important source of candidate molecules for biotechnological and biomedical uses, such as the treatment of parasitic diseases.  Methods  Africanized honeybee venom from Apis mellifera was fractionated by RP-C18-HPLC and the obtained melittin was incubated with promastigotes and intracellular amastigotes of Leishmania (L.) infantum. Cytotoxicity to mice peritoneal macrophages was evaluated through mitochondrial oxidative activity. The production of anti- and pro-inflammatory cytokines, NO and H2O2 by macrophages was determined.  Results  Promastigotes and intracellular amastigotes were susceptible to melittin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melittin; Apis mellifera; Leishmania; Leishmaniasis; Peptides; Toxins; Antiparasitic; Cytokines.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100301

ヨーロッパの非加熱食肉製品の特性について
Provedor de dados: 19
Autores: 三上, 正幸; 島田, 謙一郎; 関川, 三男; MIKAMI, Masayuki; SHIMADA, Ken-ichiro; SEKIKAWA, Mitsuo.
Some properties of the non-heated meat products in European countries were analyzed. The dry fermented sausages used were Spanish salami (small，medium and large size)，Chorizo， Pepper salami， Italian salami，Hungarian salami and Serrano ham. Viable bacterial counts were small number (10³-10⁵CFU/g) in the hard type sausages and were large number (10⁷ -10⁸CFU/g) in the semi soft type sausages. The pH of the dry fermented sausages inoculated with mold was 5.4-6.7 and was higher than 5.1 of Chorizo which was not inoculated with mold. Peptides and total free amino acids content were 634.0 - 2，240.7 mg/100g products and 404.8 - 2，413.4 mg/100g products， respectively. These were high numbers in Serrano ham. Nitrite ion contents were low level (0.3-4.3 ppm) in...
Palavras-chave: 発酵ソーセージ; 乾塩ハム; ペプチド; 遊離アミノ酸; Dry fermented sausages; Dry cured ham; Peptides; Free amino acids.
Ano: 2001
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2992

Bioactive peptides from marine sources: pharmacological properties and isolation procedures
Provedor de dados: 20
Autores: Aneiros, A.; Garateix, A.
Marine organisms represent a valuable source of new compounds. The biodiversity of the marine environment and the associated chemical diversity constitute a practically unlimited resource of new active substances in the field of the development of bioactive products. In this paper, the molecular diversity of different marine peptides is described as well as information about their biological properties and mechanisms of action is provided. Moreover, a short review about isolation procedures of selected bioactive marine peptides is offered. Novel peptides from sponges, ascidians, mollusks, sea anemones and seaweeds are presented in association with their pharmacological properties and obtainment methods.
Tipo: Journal Contribution
Palavras-chave: Marine environment; Marine organisms; Peptides; Bioactive compounds; Marine environment; Peptides; Http://aims.fao.org/aos/agrovoc/c_4610; Http://aims.fao.org/aos/agrovoc/c_5691.
Ano: 2004
URL: http://hdl.handle.net/1834/2921

Summary Proceedings of the Second Coordinators' Meeting on Peanut Stripe Virus
Provedor de dados: 30
Palavras-chave: Viruses; Groundnuts; Genotypes; Yield losses; ELISA; Peptides; Quarantine; Legumes; Germplasm; HPLC.
Ano: 1989
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4070

Fish protein hydrolysate in diets for Nile tilapia post-larvae
Provedor de dados: 86
Autores: Silva,Thiberio Carvalho da; Rocha,Joana D’Arc Mauricio; Moreira,Pedro; Signor,Altevir; Boscolo,Wilson Rogerio.
Abstract: The objective of this work was to determine the apparent digestibility coefficient (ADC) of crude protein, crude energy, fat, and dry matter of fish protein hydrolysate (FPH), made of by-products of Nile tilapia (Oreochromis niloticus) and whole sardines (Cetengraulis edentulus), and to evaluate the productive performance and muscle fiber growth of Nile tilapia post-larvae. Two trials were conducted, the first one to determine the digestibility in 120 fingerlings (70.0±2.0 g), and the second one to evaluate the productive performance of 375 post-larvae, with three days of age, which were distributed in 25 aquaria with 30 L of useful volume. Five diets were prepared based on vegetable ingredients, to which fish were included at 0, 2, 4, 6, and 8%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cetengraulis edentulus; Oreochromis niloticus; Digestibility; Fish meal; Nutrition; Peptides; Pisciculture.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000700485

SCORPION VENOM: NEW PROMISE IN THE TREATMENT OF CANCER
Provedor de dados: 37
Autores: GÓMEZ RAVE,Lyz Jenny; MUÑOZ BRAVO,Adriana Ximena; SIERRA CASTRILLO,Jhoalmis; ROMÁN MARÍN,Laura Melisa; CORREDOR PEREIRA,Carlos.
ABSTRACT Cancer is a public health problem due to its high worldwide morbimortality. Current treatment protocols do not guarantee complete remission, which has prompted to search for new and more effective antitumoral compounds. Several substances exhibiting cytostatic and cytotoxic effects over cancer cells might contribute to the treatment of this pathology. Some studies indicate the presence of such substances in scorpion venom. In this review, we report characteristics of the principal scorpion venom components found in recent literature and their potential activity against tumor cells. There are different toxin groups present in the venom, and it seems that their mode of actions involves ionic channel blocking, disruption of the cell membrane...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-infective agents; Antineoplastic agents; Arachnida; Molecular biology; Peptides.
Ano: 2019
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000200213

Proteins and Peptides at the Interfaces of Nanostructures
Provedor de dados: 42
Autores: BRITO,ADRIANNE M.M.; BELLETI,ELISANGELA; MENEZES,LUCIVALDO R.; LANFREDI,ALEXANDRE J.C.; NANTES-CARDOS,ISELI L.
Abstract:  The present review focuses on the proteins and peptides at the interfaces of nanostructured metals and semiconductors as a result of their use in synthesis in-situ and functionalization of nanostructures. We start the review with an introduction about the peculiar properties of nanostructured materials and their applications. In the following, the chemical and structural properties of peptides and proteins that allow their use as reducing, stabilizing, and functionalization agents are discussed. P﻿roteins and peptides have not only the chemical groups for the metal ion reducing but also provide templates for directing the crystalline growing of nanostructures to the desired shapes and sizes. Proteins and peptides are also used mainly for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Controlled synthesis; Noble-metals; Nanomaterials; Catalysis; Proteins; Peptides.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700402

Relevant peptides of Taenia crassiceps for the diagnosis of bovine cysticercosis by immunoblot
Provedor de dados: 45
Autores: Silva,L.F.; Pinto,P.S.A.; Ducas,C.T.S.; Santos,T.O.; Nieto,E.C.A.; Peixoto,R.P.M.G.
Given the limited knowledge about the diagnosis of bovine cysticercosis by immunoblot, the aim of this study was to assess the applicability of this test, identifying key peptides with diagnostic value. Immunoblot assays were performed using total larval antigen of Taenia crassiceps and 60 sera of positive bovines for cysticercosis (30 naturally and 30 experimentally infected with T. saginata eggs), 30 sera of negative bovines for cysticercosis and 30 sera of bovines with other diseases (fascioliasis, hydatidosis and tuberculosis). The peptides of greater diagnostic importance, in descending order of accuracy (%), were as follows: 6-8kDa (90.8%), 129-143kDa (74.2%), 99-105kDa (71.7%) and 14-19kDa (71.1%). Cross-reactions, due to fascioliasis and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine cysticercosis; Diagnosis; Immunoblot; Taenia crassiceps; Peptides.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300891

An integrative view to structure, function, ontogeny and phylogenetical significance of the male genital system in Pomacea canaliculata (Caenogastropoda, Ampullariidae)
Provedor de dados: 47
Autores: Gamarra-Luques,C.; Winik,B.C.; Vega,I.A.; Albrecht,E.A.; Catalan,N.M.; Castro-Vazquez,A.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spermatogenesis; Steroids; Peptides; Imposex; Xenobiotics.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200012

Histidines 13 and 14 in the A&amp;#946; sequence are targets for inhibition of Alzheimer's disease A&amp;#946; ion channel and cytotoxicity
Provedor de dados: 48
Autores: DÍAZ,JUAN CARLOS; LINNEHAN,JOHN; POLLARD,HARVEY; ARISPE,NELSON.
The fact that Alzheimer's beta amyloid (A&amp;#946;) peptides forms cation channels in lipid bilayers was discovered during the course of our experiments in the laboratory of "Guayo" Rojas at NIH in Bethesda, Maryland (USA). Recently, we found that the A&amp;#946; ion channel could be blocked selectively with small peptides that copy the amino acid sequence of the predicted mouth region of the A&amp;#946; channel pore. We now have searched for the essential amino acid residues required for this blocking effect by mutations. We found that the ability of peptides to block A&amp;#946; channel activity could be lost by replacement of histidines 13 and 14 by alanine or lysine. The amino acid substitution also resulted in the loss of the capacity of the peptides...
Tipo: Journal article
Palavras-chave: Alzheimer's disease; Beta amyloid; Ion channel blockers; Peptides; Histidine; Toxicity.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300007

The complete sequence of the mitochondrial genome of the Chinook salmon, Oncorhynchus tshawytscha
Provedor de dados: 48
Autores: WILHELM,VIVIAN; VILLEGAS,JAIME; MIQUEL,ÁLVARO; ENGEL,ESTEBAN; BERNALES,SEBASTIÁN; VALENZUELA,PABLO D.T.; BURZIO,LUIS O.
The complete sequence of the mitochondrial genome of Chinook salmon, Oncorhynchus tshawytscha, has been determined. The circular genome consisting of 16,644 base pairs encodes thirteen proteins, the 12S and 16S ribosomal RNAs, and 22 transfer RNAs. These genes are ordered in the same way as most other vertebrates. The nucleotide and amino acid sequences of the ribosomal RNAs and the thirteen protein-coding genes were compared with those of other salmonids such as Oncorhynchus mykiss, Salmo salar, Salvelinus fontinalis, Salvelinus alpinus and Coregonus lavaretus. The sequence features of the control region (D-loop), the origin of L-strand replication and a putative peptide codified by the 16S mitochondrial RNA are described and discussed.
Tipo: Journal article
Palavras-chave: Salmonids; Mitochondria; Genome; Peptides; 16S rRNA.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200012

Antithrombotic Activity of Milk Protein Hydrolysates by Lactic Acid Bacteria Isolated from Commercial Fermented Milks
Provedor de dados: 52
Autores: Pérez-Escalante,Emmanuel; Jaimez-Ordaz,Judith; Castañeda-Ovando,Araceli; Contreras-López,Elizabeth; Añorve-Morga,Javier; González-Olivares,Luis Guillermo.
ABSTRACT In the last decade, thrombosis has been one of the pathologies with high incidence creating great concern in Health Institutes all around the world. Considering this, the aim of this research was to determine the antithrombotic activity of peptides released during lactic fermentation. Reconstituted skim milk powder was fermented by Lactobacillus casei Shirota and Lactobacillus johnsonii LA1 isolated from commercial fermented milks. The hydrolysis degree and proteolytic profile were analyzed by trinitrobenzenesulfonic acid spectrophotometry method and by peptide polyacrylamide electrophoresis gel separation. The milk fermented by Lactobacillus casei Shirota exhibited a higher concentration of free amino groups than that fermented by Lactobacillus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antithrombotic activity; Peptides; Lactic fermentation; Probiotic bacteria; Commercial fermented milks.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100506

Production of Hydrolysate of Okara Protein Concentrate with High Antioxidant Capacity and Aglycone Isoflavone Content
Provedor de dados: 52
Autores: Figueiredo,Vitória Ribeiro Garcia de; Justus,Ariana; Pereira,Dafne Garcia; Georgetti,Sandra Regina; Ida,Elza Iouko; Kurozawa,Louise Emy.
Abstract This work aimed to evaluate the enzymatic hydrolysis of okara protein concentrate with respect to degree of hydrolysis (DH) in order to obtain a protein hydrolysate with high antioxidant capacity and aglycones isoflavone content. A central composite rotatable design was carried out to evaluate the influence of temperature (40 to 70°C), enzyme:substrate ratio (0.5 to 5.0%, g/100g protein) and pH (7.0 to 9.0) on DH. The optimal condition was 55°C, pH 9 and enzyme:substrate ratio of 5.0%, resulting a DH value of 35.5%. After protein hydrolysis at optimal condition, the antioxidant capacities of hydrolysate increased from 58.29 to 383.49 μM Trolox equivalent/g solids (ABTS method) and 2.41 to 15.32 μM Trolox equivalent/g solids (FRAP method) when...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soy pulp; Peptides; Cleavage of protein; Isoflavone profile; Electrophoresis; Experimental design.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100505

Action of a pancreatin and an Aspergillus oryzae protease on whey proteins: correlation among the methods of analysis of the enzymatic hydrolysates
Provedor de dados: 52
Autores: Morais,Harriman Aley; Silvestre,Marialice Pinto Coelho; Silveira,Josianne Nicácio; Silva,Ana Cristina Simões e; Silva,Viviane Dias Medeiros; Silva,Mauro Ramalho.
The objectives of this study were to hydrolyze whey proteins using a pancreatin and an Aspergillus oryzae protease; to evaluate the degree of hydrolysis (DH) and the peptide profile; and to establish the correlations among the analytical methods. Ten hydrolysates were prepared at different reaction times and the highest DH was obtained by the protein content method. Good correlations (r &gt; 0.87) between the methods of formaldehyde and orthophthalaldehyde (OPA), formaldehyde and osmometry as well as osmometry and OPA were observed using pancreatin. Similar results were obtained between OPA and soluble protein content for the A. oryzae protease. The action of pancreatin produced the highest contents of di- and tripeptides (9.07, 7.12 and 6.46%) and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Whey; Protein Hydrolysates; Degree of Hydrolysis; Peptides; Pancreatin; Aspergillus oryzae Protease.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600014

Epitope of dengue virus E protein detect human antibodies associated with mild disease: a potential peptide for vaccine development
Provedor de dados: 55
Autores: Sánchez-Burgos,Gilma; Herrera-Nájera,Carla; Cedillo-Rivera,Roberto; Dumonteil,Eric.
ABSTRACT The antigenic potential of seven immunogenic peptides of the dengue virus was evaluated in the sera of patients with dengue confirmed by IgM/IgG serology. Antibodies IgM and IgG against dengue virus peptides were analyzed by ELISA in 31 dengue sero-positive and 20 sero-negative patients. The P5 peptide showed significant IgG immunoreactivity mostly in the sera of patients with dengue without warning signs in comparison with patients with dengue with warning signs, correlating with mild disease. This finding suggests that the low antibody response against P5 epitope could be a risk factor for higher susceptibility to dengue virus infection with warning signs, and that P5 could be a potential antigen for vaccine development.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Dengue virus; Epitopes; Peptides; Antibodies.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000100085

Endogenous vasopressin and the central control of heart rate during dynamic exercise
Provedor de dados: 56
Autores: Michelini,L.C.
The present article contains a brief review on the role of vasopressinergic projections to the nucleus tractus solitarii in the genesis of reflex bradycardia and in the modulation of heart rate control during exercise. The effects of vasopressin on exercise tachycardia are discussed on the basis of both the endogenous peptide content changes and the heart rate response changes observed during running in sedentary and trained rats. Dynamic exercise caused a specific vasopressin content increase in dorsal and ventral brainstem areas. In accordance, rats pretreated with the peptide or the V1 blocker into the nucleus tractus solitarii showed a significant potentiation or a marked blunting of the exercise tachycardia, respectively, without any change in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise tachycardia; V1 receptors; Brainstem areas; Peptides; Blood pressure; Training; Rats.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900012