Consulta: subjectFacets:"Peptidomics"
Registros recuperados: 3
Data/hora: 04/06/2024 16:29:34

Characterization of Leiurus abdullahbayrami (Scorpiones: Buthidae) venom: peptide profile, cytotoxicity and antimicrobial activity
Provedor de dados: 80
Autores: Erdeş,Efe; Doğan,Tuğba Somay; Coşar,İlhan; Danışman,Tarık; Kunt,Kadir Boğaç; Şeker,Tamay; Yücel,Meral; Özen,Can.
Background Scorpion venoms are rich bioactive peptide libraries that offer promising molecules that may lead to the discovery and development of new drugs.Leiurus abdullahbayrami produces one of the most potent venoms among Turkish scorpions that provokes severe symptoms in envenomated victims.Methods In the present study, the peptide profile of the venom was investigated by electrophoretic methods, size-exclusion and reversed-phase chromatography and mass spectroscopy. Cytotoxic and antimicrobial effects were evaluated on a breast cancer cell line (MCF-7) and various bacterial and fungal species.Results Proteins make up approximately half of the dry weight of L. abdullahbayrami crude venom. Microfluidic capillary electrophoresis indicated the presence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venom; Toxin; Peptide; Leiurus abdullahbayrami; Microfluidic capillary; Electrophoresis; Peptidomics; Venomics; Cytotoxicity; Antimicrobial activity; Turkey.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200338

Peptidomic investigation of Neoponera villosa venom by high-resolution mass spectrometry: seasonal and nesting habitat variations
Provedor de dados: 80
Autores: Cologna,Camila Takeno; Rodrigues,Renata Santos; Santos,Jean; Pauw,Edwin de; Arantes,Eliane Candiani; Quinton,Loïc.
Abstract  Background: Advancements in proteomics, including the technological improvement in instrumentation, have turned mass spectrometry into an indispensable tool in the study of venoms and toxins. In addition, the advance of nanoscale liquid chromatography coupled to nanoelectrospray mass spectrometry allows, due to its high sensitivity, the study of venoms from species previously left aside, such as ants. Ant venoms are a complex mixture of compounds used for defense, predation or communication purposes. The venom from Neoponera ants, a genus restricted to Neotropical regions, is known to have cytolytic, hemolytic, antimicrobial and insecticidal activities. Moreover, venoms from several Neoponera species have been compared and differences in their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ant venom; Peptidomics; Ponericins; Venom comparison; Antimicrobial peptides.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100303

Initial peptidomic profiling of Brazilian sea urchins: Arbacia lixula, Lytechinus variegatus and Echinometra lucunter
Provedor de dados: 80
Autores: Sciani,Juliana Mozer; Emerenciano,Andrews Krupinski; Silva,José Roberto Machado Cunha da; Pimenta,Daniel Carvalho.
Abstract  Background  Sea urchins can be found throughout the Brazilian coast and are reported to be one of the major causes of marine accidents on the shoreline. Although not lethal, these accidents are reported to be extremely painful. In order to understand the toxinology of the Brazilian urchins, a peptidomic approach was performed aiming to characterize the naturally occurring peptides in both the coelomic fluid and the spine.  Methods  Animals were collected without gender distinction and samples of the coelomic fluid and spines extracted were analyzed by RP-HPLC and mass spectrometry for peptide de novo sequencing.  Results  Several peptides were identified either in the coelomic fluid or the spine extract (except for E. lucunter). The peptide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peptides; Toxins; Sea urchin; Peptidomics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100313