Consulta: subjectFacets:"Plasminogen activation"
Registros recuperados: 2
Data/hora: 09/06/2024 18:17:13

A study of the interaction between Helicobacter pylori and components of the human fibrinolytic system
Provedor de dados: 56
Autores: Yarzábal,A.; Avilán,L.; Hoelzl,K.; Muñoz,M. de; Puig,J.; Kansau,I.
The interaction of plasminogen, tissue plasminogen activator (t-PA) and urokinase with a clinical strain of Helicobacter pylori was studied. Plasminogen bound to the surface of H. pylori cells in a concentration-dependent manner and could be activated to the enzymatic form, plasmin, by t-PA. Affinity chromatography assays revealed a plasminogen-binding protein of 58.9 kDa in water extracts of surface proteins. Surface-associated plasmin activity, detected with the chromogenic substrate CBS 00.65, was observed only when plasminogen and an exogenous activator were added to the cell suspension. The two physiologic plasminogen activators, t-PA and urokinase, were also shown to bind to and remain active on the surface of bacterial cells. epsilon-Aminocaproic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Plasminogen; T-PA; Urokinase; Plasminogen activation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900004

Modulation of staphylokinase-dependent plasminogen activation by mono- and divalent ions
Provedor de dados: 56
Autores: Yarzábal,A.; Serrano,R.L.; Puig,J.
The effect of several ions (Cl-, Na+, K+, Ca2+) on the rate of plasminogen (Pg) activation by recombinant staphylokinase (rSTA) is reported. Both monovalent and divalent ions affect the rate at which Pg is activated by rSTA, in a concentration-dependent manner (range 0-100 mM). In almost all cases, a decrease of the initial velocity of activation was observed. Cl- showed the most striking inhibitory effect at low concentrations (64% at 10 mM). However, in the presence of a fibrin surface, this inhibition was attenuated to 38%. Surprisingly, 10 mM Ca2+ enhanced the Pg activation rate 21% when a polymerized fibrin matrix was present. These data support the idea that ions can modulate the rate of Pg activation through a mechanism that may be associated with...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Staphylokinase; Plasminogen activation; Fibrinolysis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100005