Consulta: subjectFacets:"Polymerase chain reaction"
Registros recuperados: 77
Data/hora: 04/06/2024 07:02:06

Designing and validation of genus-specific primers for human gut flora study
Provedor de dados: 69
Autores: Rekha,Rani; Alam Rizvi,Moshahid; Jaishree,Paul.
The aim of this study, was to design and validate 16S rRNA targeted oligonucleotide genus specific primers for amplifying the predominant members of gut flora using polymerase chain reaction. Primers were validated against human faecal samples. Gut flora of a normal individual was compared with that of two diseased individuals. Our observations showed that the genera Lactobacillus, Bacteroides, Peptococcus, Bifidobacterium, and E. coli were invariably present in all studied subjects however, the absence of butyrate producing bacteria Ruminococcus and Peptostreptococcus were significant. Presence of the members of the genus, Campylobacter in both the diseased samples were also unusual.
Tipo: Journal article
Palavras-chave: 16S rRNA based primers; Anaerobic bacteria; Diarrhoea patient; Polymerase chain reaction.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500005

Deletion of DNA sequences of using a polymerase chain reaction based approach
Provedor de dados: 69
Autores: Pérez-Pinera,Pablo; Menéndez-González,Manuel; Vega,José Antonio.
We developed a simple, rapid and reliable method to delete DNA fragments in plasmids using a polymerase chain reaction based amplification of the circular DNA sequence that excludes the fragment to be deleted. The primers are designed to contain a non-complementary 5' sequence consisting of a restriction enzyme target sequence. Following PCR amplification, the plasmid is digested with Dpn I to eliminate the template DNA, with the chosen restriction enzyme, and ligated. The only limitation is the selection of the restriction enzyme target sequence that must not be present in the original plasmid. The method is straightforward in its execution and success relies on a meticulous primer design that permits us obtain 100% of transformants containing the desired...
Tipo: Journal article
Palavras-chave: Deletion; Mutagenesis; Polymerase chain reaction; Plasmid.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500018

The presence of sboA and spaS genes and antimicrobial peptides subtilosin A and subtilin among Bacillus strains of the Amazon basin
Provedor de dados: 74
Autores: Velho,Renata Voltolini; Basso,Ana Paula; Segalin,Jeferson; Costa-Medina,Luis Fernando; Brandelli,Adriano.
This report demonstrates the usefulness of PCR for the genes spaS and sboA as a means of identifying Bacillus strains with a potential to produce subtilin and subtilosin A. One collection strain and five Bacillus spp. isolated from aquatic environments in the Amazon basin were screened by PCR using primers for sboA and spaS designed specifically for this study. The sequences of the PCR products showed elevated homology with previously described spaS and sboA genes. Antimicrobial peptides were isolated from culture supernatants and analyzed by mass spectrometry. For all samples, the mass spectra revealed clusters with peaks at m/z 3300-3500 Da, corresponding to subtilosin A, subtilin and isoforms of these peptides. These results suggest that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus; Bacteriocin; Polymerase chain reaction; Subtilin; Subtilosin A.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100014

Non-detection of Epstein-Barr virus and human papillomavirus in a region of high gastric cancer risk indicates a lack of a role for these viruses in gastric carcinomas
Provedor de dados: 74
Autores: Yuan,Xiao-yan; Wang,Ming-yi; Wang,Xiao-yan; Chang,Ai-ying; Li,Jie.
Gastric mucosa tissue was collected from patients with gastroduodenal diseases in a region of norrteastern China showing a high risk of gastric cancer incidence. The presence of EBV and HPV were assayed to investigate the relationship between gastric carcinomas and virus infection. Neither EBV nor HPV DNA was detected in tissue from the patients. The role of EBV and HPV in gastric cancer is not well understood and still needs to be clarified.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Epstein-Barr virus; Polymerase chain reaction; Gastric carcinoma.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200007

Validação de métodos qualitativos: uma abordagem para o ensaio de PCR e para a análise microbiológica.
Provedor de dados: 14
Autores: MAGARELLI, G.; POLEZ, V. L. P.; BEMQUERER, M. P.; MALAQUIAS, J. V.
bitstream/item/226049/1/doc-novo-376.pdf
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Metódo analítico; Validação; Método de Análise; Microrganismo; Polymerase chain reaction.
Ano: 2021
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1134429

Development of a polymerase chain reaction for the detection of abalone herpesvirus infection based on the DNA polymerase gene
Provedor de dados: 5
Autores: Chen, M.h.; Kuo, S. T.; Renault, Tristan; Friedman, C. S.; Chang, P. H.
A 5781-base pair (bp) fragment of genomic DNA from the Taiwanese abalone herpesvirus was obtained and showed 99% (5767/5779) homology in the nucleotide sequence and 99% (1923/1926) in the amino acid sequence with the DNA polymerase gene of the abalone herpesvirus strain Victoria/AUS/2007. Homology of the amino acid sequence with the DNA polymerase of ostreid herpesvirus 1 was 30% (563/1856). In this study, a PCR-based procedure for detecting herpesvirus infection of abalone, Haliotis diversicolor supertexta, in Taiwan was developed. The method employed primer sets targeting the viral DNA polymerase gene, and was able to amplify DNA fragments of the expected size from infected samples. Primer sets of 40f and 146r were designed for amplification of an...
Tipo: Text
Palavras-chave: Herpes virus; Abalone; Haliotis diversicolor supertexta; DNA polymerase; Polymerase chain reaction.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00098/20952/19675.pdf

The development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of abalone herpesvirus DNA
Provedor de dados: 5
Autores: Chen, Meilin; Kuo, S. T.; Renault, Tristan; Chang, P. H.
A loop-mediated isothermal amplification (LAMP) assay was developed for the detection of abalone herpesvirus DNA. Two pairs of primers were designed, based on the sequence of the DNA polymerase gene of abalone herpesvirus. The reaction temperature and time were optimized to 63 °C and 60 min, respectively. LAMP amplicons were analyzed by 2% agarose gel electrophoresis or by visual inspection of a colour change emitted by fluorescent dye. The method developed was specific for the detection of abalone herpesvirus, without cross-reactions with other tested herpesviruses including ostreid herpesvirus 1 (OsHV-1), European eel herpesvirus, koi herpesvirus (KHV) and an avian herpesvirus. The LAMP assay was 100 folds more sensitive than a conventional PCR and 10...
Tipo: Text
Palavras-chave: Herpesvirus; Abalone; Haliotis diversicolor supertexta; Polymerase chain reaction; Loop-mediated isothermal amplification; SYBR green PCR.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00166/27690/25882.pdf

Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment
Provedor de dados: 5
Autores: Morvezen, Romain; Cornette, Florence; Charrier, Gregory; Guinand, Bruno; Lapegue, Sylvie; Boudry, Pierre; Laroche, Jean.
We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops.
Tipo: Text
Palavras-chave: Microsatellites; Polymerase chain reaction; Parentage; Assignment; Bivalve mollusc.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00137/24822/22907.pdf

ISOLATION OF AN OPHIDIAN PARAMYXOVIRUS (OPMV) IN A CAPTIVE RATTLESNAKE (Crotalus durissus terrificus) FROM BOTUCATU, SÃO PAULO STATE, BRAZIL
Provedor de dados: 79
Autores: NOGUEIRA,M. F.; BARRELLA,T. H.; SILVA,R. J. DA; LOPES,C. A. M.; ARAÚJO JÚNIOR,J. P.
This study reports the isolation of an Ophidian Paramyxovirus (OPMV) in sputum of a captive rattlesnake (Crotalus durissus terrificus) kept in a serpentarium located in Botucatu, São Paulo State, Brazil. Polymerase chain reaction (PCR) and nested-PCR were performed for the identification of the isolated virus.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Crotalus durissus terrificus; Rattlesnake; Paramyxovirus; Ophidian Paramyxovirus; Hemagglutination; Polymerase chain reaction.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000100013

American cutaneous leishmaniasis in dogs from an endemic urban area in Cianorte municipality, Paraná State, Brazil
Provedor de dados: 80
Autores: Cerino,DA; Veloso,J; Perles,TF; Zanzarini,PD; Lonardoni,MVC; Silveira,TGV.
American cutaneous leishmaniasis (ACL) was investigated in dogs from an urban endemic area in Cianorte, Paraná state, Brazil. Of 169 studied dogs, none presented suspected ACL lesions. Eleven animals (6.6%) had anti-Leishmania braziliensis antibodies (titers &gt; 40) detected by the immunofluorescent antibody test (IFAT) while four (2.4%) showed L. braziliensis-complex DNA by the polymerase chain reaction (PCR). Although no associations were found between IFAT or PCR results and age, sex, origin, free-roaming animals or length of residence at the address, the majority of IFAT- or PCR-positive dogs were from the urban area of the city and were allowed to roam freely beyond their neighborhood. The presence of anti-Leishmania braziliensis antibodies and L....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania braziliensis; Fluorescent antibody technique; Polymerase chain reaction; Urban park.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200007

Characterization of Clostridium perfringens isolated from mammals and birds from Guwahati city, India
Provedor de dados: 80
Autores: Rahman,Mafruza S; Sharma,RK; Borah,P; Chakraborty,A; Devi,Mandakini RK; Longjam,N.
Of the 102 samples collected from mammals and birds, both domestic and captive wild, 48 were found to be positive for Clostridium perfringens. Most of the mammal isolates (84.38%) appeared to have been collected from clinically affected animals, while 33.33% of the bird samples were from clinically affected and 21.43% from apparently healthy birds infected with C. perfringens. Isolates revealed high sensitivity to ciprofloxacin, enrofloxacin and norfloxacin. Among the isolated C. perfringens, 30 (62.50%) showed DNase production. Hemolytic activity was recorded in 14 (24.16%) of the isolates and 28 (58.33%) showed phospholipase C production. All the phospholipase C positive isolates revealed the presence of cpa gene encoding alpha (α) toxin. Of the 102...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; PCR; Α toxin; Cpa gene; Polymerase chain reaction.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100010

Detection of bovine Clostridium perfringens by polymerase chain reaction
Provedor de dados: 80
Autores: Piatti,R. M.; Ikuno,A. A.; Baldassi,L.
A polymerase chain reaction (PCR) assay to detect Clostridium perfringens alpha toxin gene (cpa) was used to identify eighty-nine C. perfringens strains obtained from bovine clinical material. The strains were biochemically characterized as C. perfringens. The isolated strains were cultured on plates containing brain heart infusion agar with 5% sheep blood under anaerobic conditions. DNA extraction was performed by boiling. The 324 bp amplification product of cpa was observed in all isolates. C. sordellii, C. botulinum, C. novyi, and C. septicum were also tested but did not produce any alpha toxin gene amplification. These findings suggest that PCR is a useful assay in identifying C. perfringens toxin types.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; PCR; Alpha toxin; Polymerase chain reaction.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200005

Prevalence and epidemiology of chronic hepatitis C among prisoners of Mato Grosso do Sul State, Brazil
Provedor de dados: 80
Autores: Pompilio,MA; Pontes,ERJC; Castro,ARCM; Andrade,SMO; Stief,ACF; Martins,RMB; Mousquer,GJ; Murat,PG; Francisco,RBL; Pompilio,SAL; Rezende,GR; Elias-Junior,E.
In Mato Grosso do Sul state, Brazil, the number of prisoners has increased in the recent years and the control of hepatitis C virus (HCV) has become more complex. The aim of the present study was to estimate the prevalence and identify the genotypes of HCV in prisoners as well as the factors associated with this infectious disease. Thereby, 443 men and 243 women from prisons were interviewed and subjected to blood collection. Anti-HCV reactive samples were analyzed by RT-PCR and genotyped. The overall seroprevalence of HCV infection was 4.8% (95%CI: 3.4 to 6.8%). Furthermore, the prevalence was higher in: men, injecting drug users, tattooed persons, those who were more than 50 years old, individuals who have been arrested multiple times, people with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C; Genotype; Polymerase chain reaction; Epidemiology; Prisoners.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200013

Trypanosomatids in dogs belonging to individuals with chronic Chagas’ disease living in Botucatu town and surrounding region, São Paulo State, Brazil
Provedor de dados: 80
Autores: Lucheis,S. B.; Da Silva,A. V.; Araújo Jr,J. P.; Langoni,H.; Meira,D. A.; Marcondes-Machado,J.
Among domestic animals, dogs are considered to be the major reservoirs of trypanosomatids and, due to their proximity to man, the presence of these parasites in dogs is an alert to actions aiming at triatomine control. Fifty dogs (26 males and 24 females), aged from 2 months to 15 years, belonging to 30 chronic Chagas’ disease individuals from 15 different municipalities in the western region of São Paulo State, Brazil, were subjected to blood collection for the following tests: artificial xenodiagnosis, blood culture, and Polymerase Chain Reaction (PCR). Forty-three (86%) out of 50 dogs were positive to at least one of the tests performed; 34 (68%) were positive to xenodiagnosis, 30 (60%) to blood culture, and 25 (50%) to PCR for T. cruzi and/or T....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; T. rangeli; Dog; Xenodiagnosis; Blood culture; Polymerase chain reaction.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400009

Sequence analysis of the Rhop-3 gene of Plasmodium berghei and P. chabaudi, reactivity of Rhop-3 protein within isolated rhoptries and binding of Rhop-3 to mouse erythrocytes
Provedor de dados: 19
Autores: Sam-Yellowe, Tobili Y.; Wang, Tongmin; Fujioka, Hisashi; Drazba, Judith A.; Alkawa, Masamichi; Brochak, William E.
Palavras-chave: Apicomplexa; Erytrocyte binding protein; Merozoite invasion; Plasmodium berghei; P. chabaudi; P. falciparum; P. yoelii; Polymerase chain reaction; Rhoptries; Rhop-3; Immunoelectron microscopy.
Ano: 2000
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/337

Allelic Analysis of Immunodominant Major Piroplasm Surface Protein Genes of Benign Theileria Parasites in Australian Cattle
Provedor de dados: 19
Autores: Kubota, Shuichi; Kakuda, Tsutomu; Sugimoto, Chihiro; Waltisbuhl, David; Onuma, Misao.
Palavras-chave: Allele; Benign Theileria; Piroplasm surface antigen; Polymerase chain reaction.
Ano: 1996
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/240

Epidemiological survey of Babesia gibsoni infection in dogs in eastern Japan
Provedor de dados: 19
Autores: Miyama, Takako; Sakata, Yoshimi; Shimada, Yojiro; Ogino, Shoji; Watanabe, Malaika; Itamoto, Kazuhito; Okuda, Masaru; Verdida, Rodolfo A.; Xuan, Xuenan; Nagasawa, Hideyuki; Inokuma, Hisashi.
To determine the distribution of Babesia gibsoni infection in dogs in the eastern part of Japan, an epidemiological survey of dogs suspected of having B. gibsoni infection was attempted using the polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Thirty-five of 115 such dogs (30.4%) were positive by PCR and/or ELISA. The 35 positive dogs consisted of 28 Tosa dogs, 4 American Pit Bull Terriers, and 3 mongrel dogs in Aomori, Fukushima, Ibaraki, Gunma, Chiba, Tokyo, Kanagawa, and Nagano Prefectures. The positive dogs had a significantly lower rate of tick exposure and a higher rate of bites by other dogs. Twenty-two of 35 B. gibsoni-positive dogs were infected with hemoplasma, and the rate of infection was significantly higher than...
Palavras-chave: Babesia gibsoni; Eastern Japan; Enzyme-linked immunosorbent assay; Hemoplasma; Polymerase chain reaction.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/924

Experimental infection of dogs with Babesia microti
Provedor de dados: 19
Autores: Ohmori, T.; Uetsuka, K.; Nunoya, T.
Palavras-chave: Babesia microti; Dog; Experimental infection; Polymerase chain reaction; Indirect immunofluorescence test.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3132

Diagnosis of Trypanosoma evansi by the polymerase chain reaction (PCR)
Provedor de dados: 19
Autores: Donelson, J. E.; Artama, W. T.
Palavras-chave: Trypanosoma evansi; Diagnosis; Polymerase chain reaction.
Ano: 1998
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/300

Identification of Eimeria mitis and Eimeria praecox in broiler feces using polymerase chain reaction
Provedor de dados: 89
Autores: Meireles,MV; Roberto,LO; Riera,RF.
There are few reports concerning the epidemiology of Eimeria praecox and Eimeria mitis in Brazil. In the present experiment, the polymerase chain reaction (PCR) was used to identify these species in 156 samples of broiler chicken feces from several Brazilian states and the Federal District. Oocysts present in feces samples were purified by sodium chloride flotation followed by addition of DNAzol reagent (Invitrogen®) for extraction of genomic DNA. DNA was precipitated and stored following DNAzol reagent manufacture's instructions. The primers and PCR conditions were as described by Schnitzler et al. (1999). In the 156 field samples analyzed by PCR, 70 and 45 were positive for E. praecox and E. mitis, respectively. In this study we have shown that DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; Eimeria mitis; Eimeria praecox; Epidemiology; Polymerase chain reaction.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000400010

Effect of the kappa-casein gene polymorphism, breed and seasonality on physicochemical characteristics, composition and stability of bovine milk
Provedor de dados: 96
Autores: Botaro,Bruno Garcia; Lima,Ygor Vinícius Real de; Cortinhas,Cristina Simões; Silva,Luís Felipe Prada e; Rennó,Francisco Palma; Santos,Marcos Veiga dos.
The objective of this study was to evaluate the effect of genetic polymorphism of kappa-casein, breed and seasonality on the physicochemical characteristics, composition and stability of milk in commercial dairy herds. A total of 879 milk and blood samples were collected from 603 Holstein and 276 Girolando cows, obtained during rainy and dry seasons. Milk samples were analyzed to determine the physicochemical characteristics, composition and ethanol stability, while blood samples were subjected to polymerase chain reaction to identify the kappa-casein genotype. The frequencies of genotypes AA, AB and BB of k-casein were respectively, 66.83, 31.84 and 1.33% for Holstein, and 71.38, 27.90 and 0.72% for the Girolando cows, respectively. The A allele was more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crioscopy; Dairy cow; Ethanol stability test; Genetic variant; Polymerase chain reaction; Titratable acidity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009001200022

PORCINE PARVOVIRUS INFECTIONS IN CUBA
Provedor de dados: 101
Autores: Díaz de Arce,Heidy; Pérez,L.J; Castell,Sara; Percedo,María I; Domínguez,Patricia; Frías,María T.
To obtain information about the porcine parvovirus (PPV) infection status of pigs in Cuba, tissue samples collected from ill pigs were evaluated using polymerase chain reaction (PCR). The PCR analysis showed the presence of PPV in 12 of the 34 (35.3%) field clinical samples assessed. Pigs from three of the seven swine herds of six different geographic regions were detected to be positive for PPV. A field PPV strain was isolated for the first time in the country. A swine herd showed 57 of 60 sera sampled (95%) positive for PPV specific antibodies by a commercial ELISA. This study is the first report of PPV infecting pigs in Cuban swine herds.
Tipo: Journal article
Palavras-chave: Porcine parvovirus; Polymerase chain reaction.
Ano: 2009
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2009000300007

Development of a scar marker for Pierce's Disease strains of Xylella fastidiosa
Provedor de dados: 102
Autores: Travensolo,Regiane F.; Ciapina,Luciane P.; Lemos,Eliana G. M.
The objective of this research was to develop a primer for a polymerase chain reaction specific for Xylella fastidiosa strains that cause Pierce's Disease (PD) in grapes (Vitis vinifera). The DNA amplification of 23 different strains of X. fastidiosa, using a set of primers REP1-R (5'-IIIICGICGIATCCIGGC-3') and REP 2 (5'-ICGICTTATCIGGCCTAC-3') using the following program: 94 ºC/2 min; 35 X (94 ºC/1 min, 45 ºC/1 min and 72 ºC/1 min and 30 s) 72 ºC/5 min, produced a fragment of 630 bp that differentiated the strains that cause disease in grapes from the other strains. However, REP banding patterns could not be considered reliable for detection because the REP1-R and REP 2 primers correspond to repetitive sequences, which are found throughout the bacterial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oligonucleotide; Polymerase chain reaction; PCR; REP-PCR.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000200002

TRANSFORMING GROWTH FACTOR BETA HAS DUAL EFFECTS ON MMP9 AND uPA EXPRESSION IN HTR-8/SVneo HUMAN TROPHOBLASTIC CELL LINE
Provedor de dados: 37
Autores: NOVOA HERRÁN,Sandra Susana; CASTELBLANCO,Mariela; SÁNCHEZ -GÓMEZ,Myriam; UMAÑA PÉREZ,Adriana.
ABSTRACT Invasion of trophoblast into endometrium is vital for successful pregnancy development. MMP9 and uPA are key proteases in this process, but it is still not clear the regulation of its expression by Transforming Growth Factor Beta (TGF-β), a known negative regulator of trophoblast invasion. We evaluated the effect of TGF-β on the transcriptional expression of uPA and MMP9 over time, in HTR-8/SVneo trophoblast cells cultured with or without 0.5 % fetal bovine serum, via RT qPCR. The involved transcription factors and signaling pathways were analyzed in silico, using Proscan, Enrich, PCViz and Wiki Pathway. Results showed that TGF-β temporarily regulates the expression of uPA and MMP9. Serum modified the nature of TGF-β's effects on uPA expression,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Computational biology; Matrix metalloproteinase; Placenta; Plasminogen; Polymerase chain reaction.
Ano: 2019
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000100026

Feline immunodeficiency virusinfection: a comparative study of different diagnostic techniques
Provedor de dados: 45
Autores: Mortola,E.; Oliva,G.; Risso,M.; Pecoraro,M.; Venturini,M.C.
This study evaluated an indirect immunofluorescence assay (IFA) to detect feline immunodeficiency virus infection (FIV) antibody in a comprehensive epidemiological survey of FIV in Argentina. IFA modified in our laboratory, was compared with two other immunoassays, western blot (WB) and a sandwich immunochromatographic commercial kit (SI), and also with a direct polymerase chain reaction (PCR) method that detects proviral DNA. IFA showed to be a test with high sensitivity and specificity, and could be useful as a diagnostic tool in epidemiological studies. The presence of a low percentage of results with non-specific reactivity in the IFA could be resolved with further testing or use of an alternative method.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feline immunodeficiency virus; Immunofluorescence assay; Western blot; Polymerase chain reaction.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000100003

Detection of different Brazilian strains of the bovine herpesvirus-1 (BHV-1) by polymerase chain reaction
Provedor de dados: 45
Autores: Cândido,A.L.; Martins,A.S.; Barros,P.B.; Resende,M.
A técnica da reação em cadeia pela DNA polimerase (PCR) foi usada para a amplificação rápida de um fragmento de 456bp da região única curta (Us) do genoma do BHV-1. Iniciadores de 18pb do gene da ORF1 foram usados para a amplificação das amostras-padrão e brasileiras. Uma amplificação clássica não foi bem sucedida. A amplificação foi obtida quando se escolheu uma região com baixa concentração de GC no DNA do BHV-1 e através da desnaturação térmica (95° C para 5min) seguida de ciclos térmicos (94° C por 1min e 30seg; 52° C por 1min; 72° C por 1min e 30seg; e ainda por 35 ciclos de 94° C por 1min; 52° C por 1min; 72° C por 1min e 30seg; usando um tempo de extensão final de 72° C por 5min). A clivagem com Pst I confirmou a especificidade do fragmento da ORF 1...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bovine herpesvirus 1; ORF-1; Polymerase chain reaction; Brazil.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000300007

Identification of subclinical mastitis caused by Mycoplasma spp. from screenings of bulk tanks
Provedor de dados: 45
Autores: Joaquim,S.F.; Guimarães,F.F.; Salina,A.; Junqueira,N.B.; Gomes,E.N.; Langoni,H.
ABSTRACT Mastitis caused by Mycoplasma spp., regardless of species, are considered highly contagious pathogens and, usually was not responsive to antimicrobial therapy. Five dairy herds, comprising 489 animals and 1,956 mammary glands, were used in this study. Milk samples were obtained from bulk tanks and subjected to polymerase chain reaction (PCR) for the identification of Mollicutes, Mycoplasma spp., and Mycoplasma bovis. Moreover, individual samples from cases of clinical and subclinical mastitis in quarters of the dairy herds’ animals that yielded a positive PCR upon bulk tank analysis were subjected to molecular analysis. Only one bulk tank was positive for class Mollicutes by PCR. All positive samples classified as mastitis teats had their DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Milk quality; Polymerase chain reaction; Dairy herds.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000601793

Retrospective study of Bovine herpesvirus 5 meningoencephalitis in cattle from São Paulo State, Brazil
Provedor de dados: 45
Autores: Cagnini,D.Q.; Andrade,D.G.A.; Cunha,P.H.J.; Oliveira-Filho,J.P.; Amorim,R.L.; Alfieri,A.A.; Borges,A.S.
ABSTRACT Meningoencephalitis caused by Bovine herpesvirus 5 (BoHV-5) is an important neurological disease that affects Brazilian cattle herds. The present study investigated the presence of BoHV-5 DNA in cattle diagnosed with meningoencephalitis at Faculdade de Medicina Veterinária e Zootecnia, UNESP - Univ Estadual Paulista from 1980 to 2009. The records obtained from the Large Animal Internal Medicine Service and the Animal Pathology Service were reviewed to identify clinical and epidemiological data from cattle with neurological signs. Excluding rabies cases, we found 115 cases of cattle with neurological signs that had been necropsied. Non-suppurative meningoencephalitis was diagnosed in 28 animals of the 115 initially selected based on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle diseases; Herpesvirus 5 bovine; Meningoencephalitis; Pathology; Polymerase chain reaction.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000200299

Investigation of natural infection by Leishmania in sandflies of Paraná State, Southern Brazil
Provedor de dados: 52
Autores: Scodro,Regiane Bertin de Lima; Reinhold-Castro,Kárin Rosi; Dias-Sversutti,Alessandra de Cassia; Neitzke-Abreu,Herintha Coeto; Membrive,Norberto de Assis; Kühl,João Balduíno; Silveira,Thaís Gomes Verzignassi; Teodoro,Ueslei.
The purpose of this work was to verify the occurrence of Leishmania in naturally infected sandflies. The insects were collected with Falcão, Shannon and HP light-traps, in Doutor Camargo and Maringá municipalities between November 2004 and October 2005. Of the 11,033 sandflies collected in Doutor Camargo, 2,133 surviving females were dissected, particularly those of the Nyssomyia neivai species (86.87%). In Maringá, 136 sandflies were collected, of which 79 N. whitmani females and 1 Migonemyia migonei female were dissected. The dissected insects were identified and stored in the pools of 10 specimens. The PCR was carried out on 1,190 females of N. neivai and 190 of N. whitmani from Doutor Camargo, and on 30 of N. whitmani from Maringá, using the primers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cutaneous leishmaniasis; Sandflies; Dissection; Polymerase chain reaction.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300007

Analysis of seven STR human loci for paternity testing by microchip electrophoresis
Provedor de dados: 52
Autores: Fraige,Karina; Travensolo,Regiane Fátima; Carrilho,Emanuel.
The aim of this work was to evaluate two paternity cases by microchip electrophoresis and the validation of the methodology by comparison of the results with those obtained in a commercial genetic analyzer. It was observed that when working with tetranucleotide regions, in which the minimal difference between the alleles was only four base pairs, the commercial microchip system did not present the resolution and repeatability needed. Nevertheless, the relative standard deviation was between 0 and 1.2% and the fragments detected were within the expected size ranges as described in the literature.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioanalyzer 2100; Paternity testing; Polymerase chain reaction; Short tandem repeats.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200006

Development of a polymerase chain reaction and its comparison with agar gel immunodiffusion test in the detection of bovine leukemia virus infection
Provedor de dados: 62
Autores: Camargos,Marcelo Fernandes; Stancek,Daniel; Lessa,Leandro Moreira; Reis,Jenner Karlisson Pimenta; Rocha,Maurílio Andrade; Leite,Rômulo Cerqueira.
Polymerase chain reaction (PCR) was used for bovine leukemia virus (BLV) detection in the peripheral leukocytes of the infected bovines. The primers used were designed to amplify a part of env gene of BLV. PCR products were analyzed by agarose gel electrophoresis stained by ethidium bromide. The analytical specificity of PCR was confirmed by enzymatic restriction analysis of the PCR product with Bam HI and also by nucleotide sequence analysis of three PCR samples. Sixty five animals were tested for anti-BLV antibody, by agar gel-immunodiffusion test (AGID) and for direct BLV detection by PCR. There was a 73.80% concordance rate between the two tests. Four animals positive in AGID were PCR negative, while 13 AGID negative animals were found PCR positive....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunodiffusion; Bovine leukemia virus; Gene; Polymerase chain reaction.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000500005

Comparison of multiplex PCR with serogrouping and PCR-RFLP of fliC gene for the detection of enteropathogenic Escherichia coli (EPEC)
Provedor de dados: 55
Autores: Bouzari,Saeid; Aslani,Mohammad M; Oloomi,Mana; Jafari,Anis; Dashti,Amir.
Enteropathogenic Escherichia coli (EPEC) comprise one of the six categories of diarrhoeagenic E. coli (DEC). EPEC is subgrouped into typical (tEPEC) and atypical (aEPEC). The identification of DEC cannot be based only on cultural and biochemical criteria, since they are indistinguishable from the non-pathogenic E. coli commonly found in human feces. Several PCR methods, with both single and multiple target genes, have been reported for detecting the different DEC pathotypes. In the present study five hundred E. coli isolates from children with diarrhea were subjected into multiplex PCR. Furthermore the strains were typed serologically with O antisera and their fliC gene was characterized by PCR-RFLP. The results obtained revealed that overall 41 (8.2%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Enteropathogenic Escherichia coli; Polymerase chain reaction.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400011

Seronegative cat-scratch disease diagnosed by PCR detection of Bartonella henselae DNA in lymph node samples
Provedor de dados: 55
Autores: Chondrogiannis,Konstantinos; Vezakis,Antonios; Derpapas,Michael; Melemeni,Aikaterini; Fragulidis,Georgios.
Cat scratch disease (CSD), the typical clinical manifestation of Bartonella infections usually follows a typical benign self-limited course. Nevertheless, a variety of unusual clinical manifestations and confusing imaging features can lead to misinterpretations and render the disease a diagnostic dispute. Routine laboratory tests exhibit varying reported sensitivity and are usually unhelpful in diagnosis, as serology fails in terms of specificity and/or sensitivity. Herein we report a case of seronegative Bartonella infection presenting as symptomatic suppurative lymphadenitis with abscess formation, which was surgically drained. Diagnosis was established by PCR analysis from lymph nodes samples obtained during the procedure. PCR detection of specific DNA...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Polymerase chain reaction; Cat-scratch disease; Bartonella henselae.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000100019

Chlamydia infection in patients with and without cervical intra-epithelial lesions tested by real-time PCR vs. direct immunofluorescence
Provedor de dados: 55
Autores: Oliveira,Micheline de Lucena; Amorim,Melania Maria Ramos de; Souza,Paulo Roberto Eleutério de; Albuquerque,Lúcia Cristina Bezerra de; Brandão,Lucas André Cavalcanti; Guimarães,Rafael Lima.
This study compares the detection rates of Chlamydia trachomatis by two techniques, direct immunofluorescence (IMF) and real time polymerase chain reaction (PCR), in patients with and without intra-epithelial cervical lesions (SIL) in Recife. We conducted a transversal study involving 35 women with SIL and 35 without SIL attended at Ambulatório Especializado da Mulher, Recife, Brazil. They were tested for Chlamydia trachomatis using two techniques, direct IMF or real time PCR. The rates of Chlamydia trachomatis detection were compared and the association with intra-epithelial cervical lesions was determined using the chi-square test at a 5% level of significance. Concordance between the tests was evaluated using kappa. The global prevalence of Chlamydia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlamydia trachomatis; Chlamydia infections; Fluorescent antibody technique direct; Polymerase chain reaction.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400014

Prevalence of cervical Chlamydia trachomatis infection in sexually active adolescents from Salvador, Brazil
Provedor de dados: 55
Autores: Machado,Márcia Sacramento Cunha; Silva,Bruno Fernando Borges da Costa e; Gomes,Igor Logetto Caetité; Santana,Iuri Usêda; Grassi,Maria Fernanda Rios.
The incidence of sexually transmitted diseases among adolescents is increasing worldwide. Genital Chlamydia trachomatis infection is one of the most prevalent sexually transmitted diseases in young women, and undetected disease is highly associated with long-term complications in women. Our goal was to determine the prevalence of cervical Chlamydia trachomatis infection in a sexually active population of female adolescents from Salvador, Brazil, and to describe their socio-demographic, behavioral, and clinical characteristics. 100 sexually active adolescents (10-19 years) were included in this study, between 2008 and 2010. Endocervical samples were obtained during gynecological examination. Inhouse polymerase chain reaction of cervical specimens was used...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Chlamydia trachomatis; Polymerase chain reaction; Adolescent.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000200015

Chlamydia trachomatis infection in a sample of northern Brazilian pregnant women: prevalence and prenatal importance
Provedor de dados: 55
Autores: Borborema-Alfaia,Ana Paula B. de; Freitas,Norma Suely de Lima; Astolfi Filho,Spartaco; Borborema-Santos,Cristina Maria.
There are limited data regarding prevalence of Chlamydia trachomatis infection among northern Brazilian pregnant women. OBJECTIVE: The purpose of this study was to estimate the prevalence of chlamydial infection among pregnant women in their third trimester and to determine the repercussion of this infection on their offspring. METHODS: In the first phase of this study 100 pregnant women receiving prenatal care in a local public university hospital were examined to assess the prevalence of genital C. trachomatis infection by polymerase chain reaction technique. In the second phase, 88 pregnant women were prospectively evaluated for premature rupture of membranes, puerperal consequences associated with chlamydial infection, and neonates were checked for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlamydia trachomatis; Pregnant women; Neonatal infection; Respiratory symptoms; Polymerase chain reaction; Brazil.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000500006

Clinical significance of different bacterial load ofMycoplasma pneumoniae in patients with Mycoplasma pneumoniae pneumonia
Provedor de dados: 55
Autores: Jiang,Wujun; Yan,Yongdong; Ji,Wei; Wang,Yuqing; Chen,Zhengrong.
OBJECTIVE:This retrospective study was conducted to investigate the clinical significance of differentMycoplasma pneumoniae bacterial load in patients with M. pneumoniae pneumonia (MP) in children.METHODS:Patients with MP (n=511) were identified at the Children's Hospital Affiliated to Soochow University database during an outbreak of MP between January 2012 and February 2013.RESULTS:Comparing patients with high and low bacterial load those with higher loads were significantly older (p&lt;0.01) and had fever significantly more frequently (p=0.01). Presence of wheezing at presentation was associated with low bacterial load (p=0.03). Baseline positive IgM was present in 93 (56.4%) patients with high bacterial load compared to 46 (27.8%) patients with low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma pneumoniae; Pneumonia; Polymerase chain reaction; Serology.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000200124

Characterization of rickettsia rickettsii in a case of Fatal Brazilian spotted fever in the city of Rio de Janeiro, Brazil
Provedor de dados: 55
Autores: Lamas,Cristiane; Favacho,Alexsandra; Rozental,Tatiana; Bóia,Márcio N.; Kirsten,Andrei H.; Guterres,Alexandro; Barreira,Jairo; Lemos,Elba Regina S. de.
A lethal case of Brazilian spotted fever (BSF) is presented. Clinical features were initially of gastrointestinal involvement and evolved with progression to septic shock, meningoencephalitis and death on the 6th day of illness. Indirect immunofluorescence assay (IFA) for spotted fever group rickettsia (SFGR) was non-reactive. Diagnosis was confirmed by the polymerase chain reaction (PCR) and the nucleotide sequencing of a fragment of the ompA gene showed 100% homology to Rickettsia rickettsii. BSF has not been reported in the city of Rio de Janeiro in the last three decades, and the present description should alert the clinicians to its presence in urban Rio de Janeiro, and to the differential diagnosis with dengue fever, gastroenteritis, leptospirosis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian spotted fever; Spotted fever group rickettsia; Rickettsia rickettsii; Lethal case; Rio de Janeiro city; Indirect immunofluorescence; Polymerase chain reaction; Central nervous system involvement.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000200010

Identification of putative new Escherichia coli flagellar antigens from human origin using serology, PCR-RFlP and DNA sequencing methods
Provedor de dados: 55
Autores: Tiba,Monique Ribeiro; Moura,Claúdia de; Carazzolle,Marcelo Falsarella; Leite,Domingos da Silva.
Escherichia coli has been isolated frequently, showing flagellar antigens that are not recognized by any of the 53 antisera, provided by the most important reference center of E. coli, The International Escherichia and Klebsiella Center (WHO) of the Statens Serum Institute, Copenhagen, Denmark. The objective of this study was to characterize flagellar antigens of E. coli that express non-typeable H antigens. The methods used were serology, PCR-RFLP and DNA sequencing. This characterization was performed by gene amplification of the fliC (flagellin protein) by polymerase chain reaction in all 53 standards E.coli strains for the H antigens and 20 E. coli strains for which the H antigen was untypeable. The amplicons were digested by restriction enzymes, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Antigens; Bacterial; Polymerase chain reaction; Polymorphism; Restriction fragment length.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200009

Comparison of different primes for PCR-based diagnosis of cutaneous leishmaniasis
Provedor de dados: 55
Autores: Oliveira,Diego Molina de; Lonardoni,Maria Valdrinez Campana; Teodoro,Ueslei; Silveira,Thais Gomes Verzignassi.
OBJECTIVE: The objective of this study was to analyze different primers that are commonly used in epidemiological studies for the detection of Leishmania DNA by PCR, and to compare them to the conventional direct parasite search for American cutaneous leishmaniasis (ACL) diagnosis. MATERIAL AND METHODS: Five pairs of primers, four of them derived from Leishmania kDNA sequences (MP3H-MP1L; B1-B2; LBF1-LBR1; 13A-13B), and one derived from the SL RNA (mini-exon) gene repeat (LU5A-LB3C), reported previously, were used. RESULTS: The MP3H-MP1L primers were the best at amplifying the DNA, detecting 2 fg of Leishmania spp. DNA. The 13A-13B primers presented the worst performance, detecting 512 x 10³ fg of DNA. CONCLUSION: The wide variation in the analytical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis; Polymerase chain reaction; Leishmania; DNA primers.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000300004

Molecular detection of cytomegalovirus, herpes simplex virus 2, human papillomavirus 16-18 in Turkish pregnants
Provedor de dados: 55
Autores: Dinc,Bedia; Bozdayi,Gulendam; Biri,Aydan; Kalkanci,Ayse; Dogan,Bora; Bozkurt,Nuray; Rota,Seyyal.
OBJECTIVE: Human cytomegalovirus (CMV) is the most common cause of viral intrauterine infections in the world. Herpes simplex virus type 2 (HSV-2) and human papillomavirus (HPV) are the main agents of viral sexually transmitted diseases, which cause genital ulcers and genital warts, respectively. HPV infection has been linked to the majority of the anogenital malignancies. The aim of this study was to detect the existence of CMV, HSV-2 and HPV type 16-18 in Turkish pregnants by using sensitive molecular assays. METHODS: One hundred thirty-four women (18-41 years old; mean age ± SD: 27 ± 8) applied to outpatient clinic of Obstetrics and Gynecology, in between 18th - 22nd weeks of their pregnancy and a control group of 99 healthy women (15-39 years old; mean...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pregnancy; Infection; Polymerase chain reaction.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600005

Validity of the polymerase chain reaction in the diagnosis of clinically suspected cases of American visceral leishmaniasis
Provedor de dados: 55
Autores: Pedrosa,Celia Maria Silva; Ximenes,Ricardo Arraes de Alencar; Almeida,Wendell Alexandre Pinheiro de; Rocha,Eliana Maria Mauricio da.
To test the validity of the polymerase chain reaction for diagnosing American visceral leishmaniasis, 88 suspected cases were studied. Diagnosis was confirmed in 47 (53.5%) and ruled out in 41 (46.5%) patients. Samples of bone marrow and peripheral blood were processed by polymerase chain reaction to evaluate the sensitivity and specificity of the test and its agreement beyond chance with microscopy examination. The polymerase chain reaction was positive in bone marrow of 100% of the patients with amastigotes seen with microscopy examination, and in 59.5% in those where no parasite were seen. Agreement beyond chance between visualization of the parasite in bone marrow aspirates and polymerase chain reaction was considered weak (Kappa = 0.41). Concordance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: American visceral leishmaniasis; Diagnosis; Polymerase chain reaction; Peripheral blood; Bone marrow blood.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000300003

Importance of high IgG anti-Toxoplasma gondii titers and PCR detection of T. gondii DNA in peripheral blood samples for the diagnosis of AIDS-related cerebral toxoplasmosis: a case-control study
Provedor de dados: 55
Autores: Vidal,José E; Diaz,Adrián Vladimir Hernández; Oliveira,Augusto César Penalva de; Dauar,Rafi Felicio; Colombo,Fabio Antonio; Chioccola,Vera Lucia Pereira.
BACKGROUND: Cerebral toxoplasmosis (CT) continues to cause significant morbidity and mortality in human immunodeficiency virus (HIV)-infected patients in Brazil. In clinical practice, the initial diagnosis is usually presumptive and alternative diagnosis tools are necessary. Our objective was to evaluate whether the detection of high titers of IgG anti-Toxoplasma gondii and T. gondii DNA in blood samples are associated with the diagnosis of CT. METHODS: In this case-control study we included 192 patients with HIV-1 infection: 64 patients with presumptive CT (cases) and 128 patients with other diseases (controls). Blood samples to perform indirect immunofluorescense reaction (IFI) to detect anti-T. gondii IgG antibodies and polymerase chain reaction (PCR)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasmosis; Cerebral toxoplasmosis; Polymerase chain reaction; Diagnosis; Acquired immunodeficiency syndrome; Brazil.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400009

FilmArray Meningitis/Encephalitis (ME) panel in the diagnosis of bacterial meningitis
Provedor de dados: 55
Autores: Domingues,Renan Barros; Santos,Márcio Vega dos; Leite,Fernando Brunale Vilela de Moura; Senne,Carlos.
ABSTRACT The precise diagnosis of bacterial meningitis is essential. Cytological and biochemical examination of cerebrospinal fluid (CSF) are not specific. Conventional methods for bacterial meningitis lack sensitivity or take too long for a final result. Therefore, other methods for rapid and accurate diagnosis of central nervous system infections are required. FilmArray meningitis/encephalitis (ME) panel is a PCR multiplex for simultaneous and rapid identification of 14 pathogens, including 6 bacteria, 7 viruses, and Cryptococcus. We evaluated 436 CSF samples submitted to FilmArray ME Panel. Among them, 25 cases were positive for bacteria, being Streptococcus pneumonia the most frequent (48 %). Among positive cases for bacteria, 60 % were positive only...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Meningitis; FilmArray Meningitis/Encephalitis; Cerebrospinal fluid; Polymerase chain reaction.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000600468

Quality of sputum in the performance of polymerase chain reaction for diagnosis of pulmonary tuberculosis
Provedor de dados: 55
Autores: Silva,Rosemeri Maurici da; Bazzo,Maria Luiza; Chagas,Mariana.
SETTING: faster alternative techniques are required to improve the diagnosis and control of pulmonary tuberculosis. OBJECTIVE: To evaluate the sample quality in the performance of PCR for diagnosis of pulmonary tuberculosis. METHOD: during one year, sputum samples were collected from 72 pulmonary tuberculosis patients and 12 non-tuberculosis controls, which were admitted to the Nereu Ramos hospital, Florianópolis city, Brazil. The samples were subjected to Ziehl-Neelsen-stained sputum smear microscopy and Lowestein-Jensen medium culture, which were defined as gold standard tests for mycobacteria, and polymerase chain reaction (PCR). Those samples that presented more than 40% of viable cells and less than 25% of epithelial cells were defined as high quality...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tuberculosis; Sputum; Polymerase chain reaction.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100022

Early HHV-6 replication is associated with morbidity non-related to CMV infection after kidney transplantation
Provedor de dados: 55
Autores: Schroeder,Regina Barbosa; Michelon,Tatiana Ferreira; Garbin,Gabriela; Garcia,Valter; Silveira,Janaina Gomes da; Santos,Luciano; Neumann,Jorge; Keitel,Elizete.
Human herpesvirus type 6-(HHV-6) has been associated with morbidity after liver transplantation. OBJECTIVE: The aim of this study was to determine the HHV-6 seroprevalence among donor-recipient pairs, analyze the incidence of early active infection, its clinical manifestation, interaction with CMV, and the related morbidity in the first year after kidney transplantation. METHODS: 46 donor-recipient pairs had IgG evaluated by ELISA before transplantation: HHV-6(Pambio - USA) and CMV-(Roche - USA). A frozen whole blood sample collected weekly (from the 1st to the 6th week) was retrospectively tested for HHV-6 viral load (VL) determination by real time quantitative PCR (qPCR, Nanogen - Italy). Patients were preemptively surveyed for CMV by pp65 antigenemia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytomegalovirus infections; Herpesvirus 6/human; DNA virus infections; Clinical diagnosis; Polymerase chain reaction.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000200007

Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women
Provedor de dados: 55
Autores: de-Paris,Fernanda; Machado,Alice Beatriz Mombach Pinheiro; Gheno,Tailise Conte; Ascoli,Bruna Maria; Oliveira,Kátia Ruschel Pilger de; Barth,Afonso Luis.
Streptococcus agalactiae or group B Streptococcus (GBS) is one of the most important causal agents of serious neonatal infections. Numerous assays have been evaluated for GBS screening in order to validate a fast and efficient method. The aim of this study was to compare the culture technique (established as the gold standard) with the molecular method of polymerase chain reaction (PCR) with specific primers (atr gene). Two hundred and sixty-three samples were analyzed. Vaginal samples were collected, according to the Centers for Disease Control and Prevention (CDC) recommendations, from women over 35 weeks of pregnancy at Hospital de Clínicas de Porto Alegre (HCPA). Two different extraction methods were tested in all samples collected. PCR technique...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus agalactiae; Polymerase chain reaction; Culture; Pregnancy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400004

β-lactamase producing enterobacteria isolated from surveillance swabs of patients in a cardiac intensive care unit in Rio de Janeiro, Brazil
Provedor de dados: 55
Autores: Vasques,Marcia Regina G; Bello,Alexandre Ribeiro; Lamas,Cristiane da Cruz; Correa,Juarez; Pereira,José Augusto Adler.
There is a high incidence of infections caused by betalactamase-producing Gram-negative microorganisms in Brazil. These organisms are of clinical and epidemiological importance, since their mobile genetic elements facilitate cross-infection. The present study was conducted in sentinel rectal swabs from patients admitted to a cardiac surgery hospital in Rio de Janeiro, from January through December 2007, in a consecutive manner. The aim of the study was to characterize the genotype and phenotype of these isolates from colonized patients. Biochemical tests, antimicrobial susceptibility tests, a confirmatory test for the expression of extended spectrum betalactamase (ESBL) production and polymerase chain reaction for the blaTEM, blaSHV, CTX-M1, Toho-1 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymerase chain reaction; Infection control; Enterobacteriaceae infections.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100006

Phenotypic and genotypic characterization of Rhodococcus equi isolated from sputum
Provedor de dados: 55
Autores: Silva,Paulo da; Santos,Adolfo Carlos Barreto; Sato,Daisy Nakamura; Silva,Jaqueline Otero; Medeiros,Marta Inês Cazentini; Carneiro,Ana Maria Machado; Leite,Sergio Roberto de Andrade; Leite,Clarice Queico Fujimura.
INTRODUCTION: Rhodococcus equi is an opportunistic pathogen, causing rhodococcosis, a condition that can be confused with tuberculosis. Often, without identifying M. tuberculosis, physicians initiate empiric treatment for tuberculosis. R. equi and M. tuberculosis have different susceptibility to drugs. Identification of R. equi is based on a variety of phenotypic, chromatographic, and genotypic characteristics. OBJECTIVE: This study aimed to characterize bacterial isolates from sputum samples suggestive of R. equi. METHODS: The phenotypic identification included biochemical assays; thin-layer chromatography (TLC) and polymerase chain reaction (PCR) were used for genotypic identification. RESULTS: Among 78 Gram-positive and partially acid-fast bacilli...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymerase chain reaction; Rhodococcus equi; Mycolic acids.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000500001

Nosocomial and community infections due to class A extended-spectrum β-lactamase (ESBLA)-producing Escherichia coli and Klebsiella spp. in southern Brazil
Provedor de dados: 55
Autores: Wollheim,Claudia; Guerra,Ivani Maria F; Conte,Vania D; Hoffman,Sheila P; Schreiner,Fernando J; Delamare,Ana Paula L; Barth,Afonso L; Echeverrigaray,Sérgio; Costa,Sérgio Olavo P da.
OBJECTIVES: To determine the prevalence of class A extended spectrum β-lactamases (ESBL)-producing Escherichia coli and Klebsiella spp., and to investigate clonality among ESBL-producing isolates of nosocomial and community infections. METHODS: The study involved 354 nosocomial infections samples and 992 community infections samples, obtained between 2003 and 2006 at Caxias do Sul, RS. The detection of ESBL was performed by the disk-diffusion test. Presence of blaCTX-M, blaSHV and blaTEM β-lactamase genes was evaluated by PCR, and genomic typing was determined by pulsed-field gel electrophoresis analysis. RESULTS: Higher frequency of ESBL-producing isolates were detected among nosocomial samples of E. coli (6.7%) and Klebsiella (43.7%), than those obtained...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-lactamases; Polymerase chain reaction; Bacterial typing techniques.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200008

Polymerase chain reaction as a useful and simple tool for rapid diagnosis of tuberculous meningitis in a Brazilian tertiary care hospital
Provedor de dados: 55
Autores: Dora,José Miguel; Geib,Guilherme; Chakr,Rafael; Paris,Fernanda de; Mombach,Alice Beatriz; Lutz,Larissa; Souza,Carolina Fischinger Moura de; Goldani,Luciano Z.
Meningitis is a severe and potentially fatal form of tuberculosis. The diagnostic workup involves detection of acid-fast bacilli (AFB) in the cerebrospinal fluid (CSF) by microscopy or culture, however, the difficulty in detecting the organism poses a challenge to diagnosis. The use of the polymerase chain reaction (PCR) in the diagnostic approach to Mycobacterium tuberculosis (MTB) meningitis has been reported as a fast and accurate method, with several commercial kits available. As an alternative, some institutions have been developing inexpensive in house assays. In our institution, we use an in house PCR for tuberculosis. We analyzed the performance of our PCR for the diagnosis of MTB meningitis in 148 consecutive patients, using MTB culture as gold...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculous meningitis; Diagnosis; Polymerase chain reaction; PCR; Mycobacterium tuberculosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000300015

Molecular diagnosis of cryptococcal meningitis in cerebrospinal ﬂuid: comparison of primer sets for Cryptococcus neoformans and Cryptococcus gattii species complex
Provedor de dados: 55
Autores: Martins,Marilena dos Anjos; Brighente,Kate Bastos Santos; Matos,Terezinha Aparecida de; Vidal,Jose Ernesto; Hipólito,Daise Damaris Carnietto de; Pereira-Chioccola,Vera Lucia.
Aim:This study evaluated the use of polymerase chain reaction for cryptococcal meningitis diagnosis in clinical samples.Materials and methods:The sensitivity and speciﬁcity of the methodology were evaluated using eight Cryptococcus neoformans/C. gattiispecies complex reference strains and 165 cere- brospinal ﬂuid samples from patients with neurological diseases divided into two groups: 96 patients with cryptococcal meningitis and AIDS; and 69 patients with other neurological opportunistic diseases (CRL/AIDS). Two primer sets were tested (CN4-CN5 and the multiplex CNa70S-CNa70A/CNb49S-CNb-49A that amplify a speciﬁc product for C. neoformansand another for C. gattii).Results:CN4-CN5 primer set was positive in all Cryptococcusstandard strains and in 94.8% in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryptococcal meningitis; Acquired immunodeﬁciency syndrome; Polymerase chain reaction; Cerebrospinal ﬂuid.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000100062

Infection with human papillomaviruses of sexual partners of women having cervical intraepithelial neoplasia
Provedor de dados: 56
Autores: Rombaldi,R.L.; Serafini,E.P.; Villa,L.L.; Vanni,A.C.; Baréa,F.; Frassini,R.; Xavier,M.; Paesi,S.
Epidemiological studies show that human papillomaviruses (HPV) are strongly related to cervical cancer and cervical intraepithelial neoplasias (CIN). Unlike the case for women, there are no consistent data on the natural history of HPV in the male population even though these viruses are prevalent in males. We carried out a prospective study to assess the prevalence of HPV in males as well as the factors that determine such infections in 99 male sexual partners of women with CIN. The genitalia of the males were physically examined and subjected to peniscopy for the collection of scrapings which were subjected to the polymerase chain reaction and restriction fragment length polymorphism to detect HPV. Of the 99 males sampled, 54 (54.5%) were positive for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cervical intraepithelial neoplasia; Epidemiology; Human papillomaviruses; Polymerase chain reaction.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200003

Detection of human parvovirus B19 in a patient with hepatitis
Provedor de dados: 56
Autores: Pinho,J.R.R.; Alves,V.A.F.; Vieira,A.F.; Moralez,M.O.S.; Fonseca,L.E.P.; Guz,B.; Wakamatsu,A.; Cançado,E.L.R.; Carrilho,F.J.; da Silva,L.C.; Bernardini,A.P.; Durigon,E.L.
Parvovirus B19 has been associated by some investigators with cases of severe hepatitis. The aim of the present study was to determine the presence of active parvovirus B19 infection among 129 Brazilian patients with non-A-E hepatitis. The patients were assayed for antibodies against parvovirus B19, IgM class, by ELISA. In IgM-positive cases, parvovirus B19 DNA was assayed by PCR in serum and liver tissue and parvovirus VP1 antigen in liver tissue was assayed by immunohistochemistry. Antibodies against parvovirus B19, IgM class, were detected in 3 (2.3%) of 129 patients with non-A-E hepatitis. Previous surgery and blood transfusions were reported by these 3 patients. One patient was a 56-year-old female with severe hepatitis, with antimitochondrial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parvovirus B19; Hepatitis non-A-E; Autoimmune hepatitis; Polymerase chain reaction; Immunohistochemistry.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900005

BCG lymphadenopathy detected in a BCG-vaccinated infant
Provedor de dados: 56
Autores: Barouni,A.S.; Augusto,C.; Queiroz,M.V.N.P.; Lopes,M.T.P.; Zanini,M.S.; Salas,C.E.
Large-scale vaccination with BCG, the live attenuated strain of Mycobacterium bovis, is being adopted around the world, although sporadic complications have occurred after the procedure. Lymphadenopathy is not uncommon especially in babies under one year (0.73% of vaccinated infants), but the swelling subsides within 2 months in most cases, with no medical or surgical treatment. Brazil adopted BCG vaccination program earlier in the seventies and by 1995 more than 96% of the infant population received this immunization. We report here the occurrence of lymphadenopathy in a two-year-old child vaccinated with the Brazilian BCG strain. The diagnosis was made using a lymph node biopsy and intestinal aspirates that yielded a positive mycobacterial culture. The...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bacille Calmette-Guérin (BCG); Vaccination; Polymerase chain reaction; Lymphadenopathy; Antibiotic resistance.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500011

Human papillomavirus infection among sexual partners attending a Sexually Transmitted Disease Clinic in Rio de Janeiro, Brazil
Provedor de dados: 56
Autores: Afonso,L.A.; Rocha,W.M.; Carestiato,F.N.; Dobao,E.A.; Pesca,L.F.; Passos,M.R.L.; Cavalcanti,S.M.B.
Cervical cancer is a major source of illness and death among women worldwide and genital infection with oncogenic human papillomavirus (HPV) its principal cause. There is evidence of the influence of the male factor in the development of cervical neoplasia. Nevertheless, the pathogenic processes of HPV in men are still poorly understood. It has been observed that different HPV types can be found among couples. The objective of the present study was to investigate HPV infections in female patients (n = 60 females/group) as well as in their sexual partners and to identify the concordance of HPV genotypes among them. By using the polymerase chain reaction, we detected a 95% prevalence of HPV DNA in women with cervical intraepithelial neoplasia (CIN) compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Neoplasia; Couples; Sexual partners; Polymerase chain reaction.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600533

Utilization of microsatellites for the analysis of genomic alterations in colorectal cancers in Brazil
Provedor de dados: 56
Autores: Fuzikawa,A.K.; Haddad,L.A.; da-Cunha-Melo,J.R.; Brasileiro-Filho,G.; Pena,S.D.J.
Two different pathogenetic mechanisms are proposed for colorectal cancers. One, the so-called "classic pathway", is the most common and depends on multiple additive mutational events (germline and/or somatic) in tumor suppressor genes and oncogenes, frequently involving chromosomal deletions in key genomic regions. Methodologically this pathway is recognizable by the phenomenon of loss of heterozygosity. On the other hand, the "mutator pathway" depends on early mutational loss of the mismatch repair system (germline and/or somatic) leading to accelerated accumulation of gene mutations in critical target genes and progression to malignancy. Methodologically this second pathway is recognizable by the phenomenon of microsatellite instability. The distinction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymerase chain reaction; Colorectal carcinoma; Microsatellite; P53; DCC; Microsatellite instability.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800001

HBV vaccination of HCV-infected patients with occult HBV infection and anti-HBc-positive blood donors
Provedor de dados: 56
Autores: Pereira,J.S.F.; Gonçales,N.S.L.; Silva,C.; Lazarini,M.S.K.; Pavan,M.H.P.; Fais,V.C.; Gonçales Júnior,F.L.
Anti-HBc positivity is a frequent cause of donation rejection at blood banks. Hepatitis B virus (HBV) infection may also occur in HBsAg-negative patients, a situation denoted occult infection. Similarly, very low levels of HBV-DNA have also been found in the sera of patients with chronic hepatitis C virus (HCV) infection, even in the absence of serum HBsAg. Initially we searched for HBV-DNA in serum of 100 blood donors and 50 HCV-infected patients who were HBsAg negative/anti-HBc positive by nested-PCR and by an HBV monitor commercial test for HBV-DNA. Anti-HBs seroconversion rates were measured in 100 blood donors and in 22 patients with chronic HCV infection after HBV vaccination to determine if the HBV vaccination could eliminate an occult HBV infection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymerase chain reaction; Anti-HBc-positive patients; HCV co-infection; HBV vaccination; Occult HBV infection.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400013

Cytomegalovirus infection in renal transplant recipients diagnosed by nested-PCR
Provedor de dados: 56
Autores: Aquino,V.H.; Figueiredo,L.T.M.
A prospective study of cytomegalovirus (CMV) infection was carried out on 34 renal transplant recipients managed at a General Hospital in Ribeirão Preto, SP, Brazil. Serologic tests showed that all patients were infected with CMV before renal transplantation. Two nested-PCR techniques with primers that recognize sequences of the glycoprotein B (gB) and H (gH) genes were used for CMV detection in blood and urine samples during the post-transplantation period. CMV was detected more frequently in blood samples than in urine samples (P&lt;0.001). Thirty-three patients had CMV detected at least once in blood and/or urine samples. Seven of these patients (21.2%) were diagnosed as having symptomatic CMV infection and showed a worse clinical outcome, with a higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytomegalovirus; Polymerase chain reaction; Kidney transplantation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100011

Cost-effective analysis of different algorithms for the diagnosis of hepatitis C virus infection
Provedor de dados: 56
Autores: Barreto,A.M.E.C.; Takei,K.; E.C.,Sabino; Bellesa,M.A.O.; Salles,N.A.; Barreto,C.C.; Nishiya,A.S.; Chamone,D.F.
We compared the cost-benefit of two algorithms, recently proposed by the Centers for Disease Control and Prevention, USA, with the conventional one, the most appropriate for the diagnosis of hepatitis C virus (HCV) infection in the Brazilian population. Serum samples were obtained from 517 ELISA-positive or -inconclusive blood donors who had returned to Fundação Pró-Sangue/Hemocentro de São Paulo to confirm previous results. Algorithm A was based on signal-to-cut-off (s/co) ratio of ELISA anti-HCV samples that show s/co ratio ³95% concordance with immunoblot (IB) positivity. For algorithm B, reflex nucleic acid amplification testing by PCR was required for ELISA-positive or -inconclusive samples and IB for PCR-negative samples. For algorithm C, all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C virus; Immunoblot; ELISA; Polymerase chain reaction; Algorithm; Diagnosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200009

Monitoring human cytomegalovirus viral load in peripheral blood leukocytes of renal transplant recipients by a simple limiting dilution-PCR assay
Provedor de dados: 56
Autores: Caballero,O.L.; Costa,M.C.S.L.; Trevisan,A.; Oliveira,R.M.; Viotti,E.A.; Távora,E.R.F.; Vilaça,S.S.; Sabagga,E.; de-Paula,F.J.; Távora,P.F.; Brasileiro-Filho,G.; Villa,L.L.; Simpson,A.J.G.
To assess the clinical relevance of a semi-quantitative measurement of human cytomegalovirus (HCMV) DNA in renal transplant recipients within the typical clinical context of a developing country where virtually 100% of both receptors and donors are seropositive for this virus, we have undertaken HCMV DNA quantification using a simple, semi-quantitative, limiting dilution polymerase chain reaction (PCR). We evaluated this assay prospectively in 52 renal transplant patients from whom a total of 495 serial blood samples were collected. The samples scored HCMV positive by qualitative PCR had the levels of HCMV DNA determined by end-point dilution-PCR. All patients were HCMV DNA positive during the monitoring period and a diagnosis of symptomatic infection was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymerase chain reaction; Human cytomegalovirus; Renal transplant; Diagnosis; Viral load.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200010

Rapid, specific, and sensitive detection of the ureR_1 gene in Klebsiella pneumoniae by loop-mediated isothermal amplification method
Provedor de dados: 56
Autores: Li,Chao; Fu,Gongyu; Shi,Yaoqiang; Zhang,A-Mei; Xia,Xueshan; Fang,Yue; Mao,Xiaoqin; Jiang,Jie; Song,Yuzhu; Yang,Guangying.
Klebsiella pneumoniae is one of the main pathogenic bacteria that causes nosocomial infections, such as pneumonia, urinary tract infection, and sepsis. Therefore, the rapid and accurate detection of K. pneumoniae is important for the timely treatment of infectious patients. This study aimed to establish a loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of K. pneumoniae-specific gene ureR_1 (Gene ID: 11847803). The ureR_1 gene was obtained through local and online BLAST, and the specific primers were designed for its detection. Positive reactions were observed on all 140 K. pneumoniae clinical isolates while all the 82 non-K. pneumoniae clinical isolates were negative. Plasmids with the specific gene and the mouse...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Loop-mediated isothermal amplification; Polymerase chain reaction; Klebsiella pneumoniae; Novel specific gene; Specific; Sensitive method.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300609

DNA extraction and quantification from touch and scrape preparations obtained from autopsy liver cells
Provedor de dados: 56
Autores: Ribeiro,C.N.M.; Peres,L.C.; Pina-Neto,J.M.
The objective of the present study was to develop a simplified low cost method for the collection and fixation of pediatric autopsy cells and to determine the quantitative and qualitative adequacy of extracted DNA. Touch and scrape preparations of pediatric liver cells were obtained from 15 cadavers at autopsy and fixed in 95% ethanol or 3:1 methanol:acetic acid. Material prepared by each fixation procedure was submitted to DNA extraction with the Wizard® genomic DNA purification kit for DNA quantification and five of the preparations were amplified by multiplex PCR (azoospermia factor genes). The amount of DNA extracted varied from 20 to 8,640 µg, with significant differences between fixation methods. Scrape preparation fixed in 95% ethanol provided...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autopsy; Congenital anomalies; DNA; Touch preparative; Scrape preparative; Polymerase chain reaction.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500002

A polymerase chain reaction-based assay to identify genotype F of hepatitis B virus
Provedor de dados: 56
Autores: Moraes,M.T.B.; Niel,C.; Gomes,S.A.
We have developed a polymerase chain reaction (PCR) assay which distinguishes genotype F from the other genotypes of hepatitis B virus (HBV). The method was used to characterize HBV strains isolated in urban areas of the Brazilian Amazon. DNA was amplified in 54 of a total of 78 HBsAg-positive serum samples, using universal, non-genotype-specific primers. Only 4 (7.4%) were identified as genotype F by our genotype-specific PCR assay. This proportion is notably lower than that previously reported in Argentina, Venezuela, Peru, and Central America.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hepatitis B virus; Genotype F; Subtype adw4; Polymerase chain reaction; Brazil.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100006

Comparison of the diagnosis of malaria by microscopy, immunochromatography and PCR in endemic areas of Venezuela
Provedor de dados: 56
Autores: Rodulfo,H; De Donato,M; Mora,R; González,L; Contreras,C.E.
Whole blood samples (N = 295) were obtained from different locations in Amazonas and Sucre States, in Venezuela. Malaria was diagnosed by microscopy, OptiMAL™ and polymerase chain reaction (PCR), with Plasmodium vivax, P. falciparum, and P. malariae being detected when possible. We identified 93 infections, 66 of which were caused by P. vivax, 26 by P. falciparum, and 1 was a mixed infection. No infection caused by P. malariae was detected. The sensitivity and specificity of each diagnostic method were high: 95.7 and 97.9% for microscopy, 87.0 and 97.9% for OptiMAL, and 98.0 and 100% for PCR, respectively. Most samples (72.2%) showed more than 5000 parasites/µL blood. The sensitivity of the diagnosis by microscopy and OptiMAL decreased with lower...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malaria; Molecular diagnosis of malaria; Microscopic diagnosis of malaria; Polymerase chain reaction; OptiMAL.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400012

PCR identification of Mycobacterium tuberculosis complex in a clinical sample from a patient with symptoms of tuberculous spondylodiscitis
Provedor de dados: 56
Autores: Ribeiro,M.A.; Barouni,A.S.; Augusto,C.J.; Augusto,M.V.S.; Lopes,M.T.P.; Salas,C.E.
A 42-year-old male complaining of thoracic spine pain was admitted to the hospital for evaluation. An X-ray and computer tomography of the thoracic spine showed spondylodiscitis of the L3 lumbar and L2-L3 intervertebral disk. The tuberculin skin test (PPD) was strongly positive. A radioscopy-guided fine needle aspirate of the affected area was cultured but did not reveal the cause of the disease. Two biopsy attempts failed to reveal the cause of the disease by culturing or by acid-fast-resistant staining (Ziehl Neelsen) of the specimens. A third biopsy also failed to detect the infectious agent by using microbiological procedures, but revealed the presence of a 245-bp amplicon characteristic of the Mycobacterium tuberculosis complex after PCR of the...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Spondylodiscitis; Tuberculosis; Polymerase chain reaction; Mycobacterium tuberculosis complex.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100001

Absence of hepatitis B virus DNA in patients with hepatitis C and non-A-E hepatitis in the State of São Paulo, Brazil
Provedor de dados: 56
Autores: Souza,L.O.; Pinho,J.R.R.; Carrilho,F.J.; da Silva,L.C.
Occult hepatitis B virus (HBV) infection has been reported as cases in which HBV DNA was detected despite the absence of any HBV serological markers or in cases in which anti-HBc antibody was the sole marker. The aim of the present study was to determine, using the polymerase chain reaction (PCR), whether HBV infection occurs in hepatitis C and non-A-E hepatitis patients without serological evidence of hepatitis B infection in São Paulo State. Two different populations were analyzed: 1) non-A-E hepatitis patients, including 12 patients with acute and 50 patients with chronic hepatic disorders without serological evidence of infection with known hepatitis viruses; 2) 43 patients previously diagnosed as hepatitis C with positive results for anti-HCV and HCV...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hepatitis B virus; Non-A-E hepatitis; Polymerase chain reaction.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100011

Risk of hepatitis B virus transmission by diagnostic hysteroscopy
Provedor de dados: 56
Autores: Santos,N.C.; Pinho,J.R.R.; Lemos,M.F.; Moreira,R.C.; Lopes,C.M.C.; Sacilotto,M.T.J.; Tacla,M.; Pinheiro,W.S.; Ramos,L.O.
Few data are available in the literature concerning the efficacy of standard hysteroscope disinfection procedures to prevent hepatitis B transmission. The aim of the present study was to determine the risk of hepatitis B virus (HBV) transmission during hysteroscopy among anti-HBc-seropositive women. Serum and hysteroscopic samples were collected from 62 women after diagnostic hysteroscopy. All samples were tested for serologic HBV markers. Polymerase chain reactions (PCR) were carried out to amplify regions C and S of the viral genome and only samples amplified by both pairs of primers were considered to be positive. Anti-HBc was repeatedly reactive in 48 (77%) of 62 serum samples, and HBsAg was detected in 8 (13%). At least one HBV serologic marker was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis B; Hysteroscopy; Disinfection; Polymerase chain reaction.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500009

Kaposi's sarcoma-associated herpesvirus infection and Kaposi's sarcoma in Brazil
Provedor de dados: 56
Autores: Ramos-da-Silva,S.; Elgui-de-Oliveira,D.; Borges,L.; Bacchi,C.E.
Kaposi's sarcoma (KS) became a critical health issue with the emergence of acquired immunodeficiency syndrome (AIDS) in the 1980s. Four clinical-epidemiological forms of KS have been described: classical KS, endemic KS, iatrogenic KS, and AIDS-associated KS. In 1994, Kaposi's sarcoma-associated herpesvirus (KSHV) or human herpesvirus type 8 was identified by Chang and colleagues, and has been detected worldwide at frequencies ranging from 80 to 100%. The aim of the present study was to evaluate the frequency of KSHV infection in KS lesions from HIV-positive and HIV-negative patients in Brazil, as well as to review the current knowledge about KS transmission and detection. For these purposes, DNA from 51 cases of KS was assessed by PCR: 20 (39.2%) cases of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kaposi's sarcoma; Human herpesvirus type 8; Kaposi's sarcoma-associated herpesvirus; Polymerase chain reaction; Viral carcinogenesis; Brazil.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500002

Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus
Provedor de dados: 58
Autores: Flatschart,Roberto B.; Resende,Maurício.
Acute and latent infections with the Brazilian LA031 strain of Aujeszky’s disease virus (ADV) were established in mice. Ultraviolet irradiated ADV administered subcutaneously was a successful way to establish latent infection. The presence of ADV was detected by PCR. Two sets of 22-mer primers were synthesized and used to amplify gG glycoprotein gene sequences in acute and latent infected trigeminal nerve ganglia. The specificity of the amplification was verified by dot-blot hybridization.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aujeszky’s disease virus; Polymerase chain reaction; Dot-blot hybridization.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400013

Prevalence of virulence genes among bulgarian nosocomial and cystic fibrosis isolates of Pseudomonas aeruginosa
Provedor de dados: 58
Autores: Mitov,Ivan; Strateva,Tanya; Markova,Boyka.
The aim of this study was to evaluate the prevalence of some virulence genes among 202 Pseudomonas aeruginosa isolates from cystic fibrosis (CF) patients (n=42) and non-CF in-patients (n=160) and to analyze the values according to the patient groups, infection localization and antimicrobial resistance. The following frequencies in all studied strains were established: algD (encoding GDP-mannose 6-dehydrogenase AlgD) - 91.1%, pilB (type IV fimbrial biogenesis protein PilB) - 23.8%, nan1 (neuraminidase) - 21.3%, lasB (elastase LasB) - 100%, plcH (haemolytic phospholipase C precursor) - 91.6%, exoS (exoenzyme S) - 62.4%, and exoU (exoenzyme U) - 30.2%. The prevalence of nan1 was significantly higher (P&lt;0.01) in CF isolates (38.1%) than that in non-CF...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas aeruginosa; Virulence genes; Polymerase chain reaction; Cystic fibrosis isolates; Non-cystic fibrosis isolates.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300008

Investigation of the association between clinical outcome and the cag pathogenicity-island and other virulence genes of Helicobacter pylori isolates from patients with dyspepsia in Eastern Turkey
Provedor de dados: 58
Autores: Ozbey,Gokben; Demirel,Ulvi; Aygun,Cem; Ertas,Hasan Basri.
The aims of our work were to determine the presence of the cag pathogenicity-island (cag PAI) and other virulence genes of Helicobacter pylori recovered from patients with gastritis and peptic ulcer, and to investigate the correlation of these virulence genes with clinical outcome. The presence of the cagA, the promoter regions of cagA, cagE, cagT, and the left end of cag-PAI (LEC), cag right junction (cagRJ), the plasticity region open reading frames (ORFs), vacA and oipA genes among 69 H. pylori isolates were determined by polymerase chain reaction. Intact cag PAI was detected in only one (1.4%) isolate. The cagA gene was identified in 52.1% and 76.2% of isolates from patients with dyspepsia (gastritis and peptic ulcer), respectively. The plasticity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Gastritis; Peptic ulcer; Cag pathogenicity-island; Polymerase chain reaction.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400034

Development of a propidium monoazide-polymerase chain reaction assay for detection of viable Lactobacillus brevis in beer
Provedor de dados: 58
Autores: Ma,Yanlin; Deng,Yang; Xu,Zhenbo; Liu,Junyan; Dong,Jianjun; Yin,Hua; Yu,Junhong; Chang,Zongming; Wang,Dongfeng.
ABSTRACT The spoilage of beer by bacteria is of great concern to the brewer as this can lead to turbidity and abnormal flavors. The polymerase chain reaction (PCR) method for detection of beer-spoilage bacteria is highly specific and provides results much faster than traditional microbiology techniques. However, one of the drawbacks is the inability to differentiate between live and dead cells. In this paper, the combination of propidium monoazide (PMA) pretreatment and conventional PCR had been described. The established PMA-PCR identified beer spoilage Lactobacillus brevis based not on their identity, but on the presence of horA gene which we show to be highly correlated with the ability of beer spoilage LAB to grow in beer. The results suggested that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propidium monoazide; Polymerase chain reaction; Beer spoilage bacteria; Lactobacillus brevis horA.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400740

The application of PCR in the detection of mycotoxigenic fungi in foods
Provedor de dados: 58
Autores: Konietzny,Ursula; Greiner,Ralf.
It is estimated that 25 to 50% of the crops harvested worldwide are contaminated with mycotoxins. Because of the toxic and carcinogenic potential of mycotoxins, there is an urgent need to develop detection methods that are rapid and highly specific. The highly advanced physico-chemical methods for the analysis of mycotoxins in use, have the disadvantage that highly sophisticated clean-up and/or derivatization procedures must be applied. An alternative could be the detection of the mycotoxigenic moulds themselves, especially as molecular techniques have been introduced recently as powerful tools for detecting and identifying fungi. PCR methods for the detection of aflatoxigenic Aspergilli, patulin-producing Penicillum and trichothecene- as well as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aflatoxin; Fumonisin; Patulin; Polymerase chain reaction; Trichothecene.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400001

Clinical and Laboratory evaluation of measleslike rash in children and young adults
Provedor de dados: 58
Autores: Stewien,Klaus Eberhard; Lima,Lourdes Rehder de Andrade Vaz de; Botosso,Viviane Fongaro; Oliveira,Maria Isabel de; Fagundes,Simone N.; Nogueira,Meri B.; Ragazzi,Selma Lopes Betta; Costa,Maria Tereza Zuluni da; Ejzenberg,Bernardo; Durigon,Edison Luiz.
A clinical and laboratory evaluation of 11 children and young adults with measleslike rash was done during the measles outbreak in the Greater São Paulo Metropolitan area at the end of 1996 and spread over the country during 1997. Measles was laboratory confirmed in 07 patients by specific IgM detection in acute serum specimens using an IgM-capture EIA, by specific IgG seroconversion in serum pairs, and by reverse transcription PCR and virus isolation in peripheral blood lymphocytes. Clinical presentations were not always classic; one of the 07 cases had received measles vaccine and corresponded to modified clinical case of measles. The 4 remaining cases were negative for measles and were diagnosed as exanthem subitum (2 cases), scarlet fever and Kawasaki...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Measles; Clinical evaluation; Laboratory investigation; Polymerase chain reaction; Measles virus isolation; Enzyme immunoassay.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400008

A quantitative analysis of Propionibacterium acnes in lesional and non-lesional skin of patients with progressive macular hypomelanosis by real-time polymerase chain reaction
Provedor de dados: 58
Autores: Cavalcanti,Silvana Maria de Morais; França,Emmanuel Rodrigues de; Magalhães,Marcelo; Lins,Ana Kelly; Brandão,Laura Costa; Magalhães,Vera.
Little is known about the etiology of progressive macular hypomelanosis, although it has been suggested that Propionibacterium acnes plays an important role. While microbiological culture is commonly employed to identify Propionibacterium acnes, new identification methods have been under investigation, amongst them polymerase chain reaction. To determine the cut-off point for the number of genome copies of Propionibacterium acnes in the lesional skin of patients with progressive macular hypomelanosis as a positive marker, employing quantitative real-time polymerase chain reaction and anaerobic culture, considered gold standard. An observational study with a comparison group, included 35 patients with dermatosis, attended at the Oswaldo Cruz University...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypopigmentation/progressive macular hypomelanosis; Propionibacterium acnes; Polymerase chain reaction; Biopsy/bacteriology; ROC curve.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200002

Prevalence of hemoglobinopathies in school children: the importance of using confirmatory methods
Provedor de dados: 60
Autores: Tavares,Cristiane Fernandes de Freitas; Guimarães,Jacqueline da Silva; Souza,Ana Maria de.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemoglobinopathy; Hemoglobin electrophoresis; High performance liquid chromatography/quantitative analysis; Polymerase chain reaction.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200361