Consulta: subjectFacets:"Primers"
Registros recuperados: 7
Data/hora: 11/06/2024 01:42:44

Diagnóstico de la presencia de roya naranja (Puccinia kuehnii (Krüger) Butler) en clones comerciales de caña de azúcar (Saccharum spp. Híbridos)  en el sureste de México.
Provedor de dados: 32
Autores: Pérez Hernández, Set.
La roya naranja (Puccinia kuehnii) es una enfermedad fungosa que afecta al cultivo de la caña de azúcar (Saccharum spp. Híbridos). La enfermedad afecta la capacidad fotosintética de la planta y reduce su crecimiento y desarrollo. El objetivo de la presente investigación fue determinar la presencia de la enfermedad de la roya naranja en clones comerciales de caña de azúcar en el sureste de México e identificar molecularmente el agente causal. En áreas de abastecimiento de los ingenios Santa Rosalía, Presidente Benito Juárez, Azuremex, Tabasco; Huixtla, Chiapas; La Joya, Campeche y San Rafael de Pucté, Quintana Roo, se colectaron hojas con síntomas y lesiones características de la roya naranja. Las hojas se cortaron en pequeñas porciones y se colocaron en...
Palavras-chave: Puccinia kuehnii; DNA; Saccharum spp.; Iniciadores; Agente causal; Primers; Causal agent; Producción Agroalimentaria en el Trópico; Maestría.
Ano: 2014
URL: http://hdl.handle.net/10521/2363

Cross-species microsatellite amplification in South American Caimans (Caiman spp and Paleosuchus palpebrosus)
Provedor de dados: 74
Autores: Zucoloto,Rodrigo Barban; Villela,Priscilla Marqui Schimidt; Verdade,Luciano Martins; Coutinho,Luiz Lehmann.
Microsatellite DNA markers have been used to assess genetic diversity and to study ecological behavioral characteristics in animals. Although these markers are powerful tools, their development is labor intensive and costly. Thus, before new markers are developed it is important to prospect the use of markers from related species. In the present study we investigated the possibility of using microsatellite markers developed for Alligator mississipiensis and Caiman latirostris in South American crocodilians. Our results demonstrate the use of microsatellite markers for Paleosuchus palpebrosus, Caiman crocodilus and Caiman yacare.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR; STR; Primers; Crocodilians; Alligatorinae.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100015

Are there differences in heterozygosity of strains obtained from intercrossed and self-fertilized onion plants?
Provedor de dados: 75
Autores: Luz,José Magno Queiroz; Machado,Daniel Lucas Magalhães; Maciel,Gabriel Mascarenhas; Freitas,Joelson André de; Oliveira,Roberta Camargos de.
ABSTRACT The commercial use of onion hybrids is preferred by producers. In contrast, the production of hybrid onion seeds is extremely inefficient. This is due to the use of lineages obtained by successive self-fertilizations assuming the effect of inbreeding depression per se. Therefore, it is necessary to understand new alternatives to reduce the effect of inbreeding depression in the strains. The objective of this study was to evaluate possible differences in polymorphism and levels of heterozygosity of strains obtained from intercrossed and self-fertilized plants. Twelve onion populations belonging to Bayer’s breeding program (Granex, IPA-1, IPA-2 and IPA-3) were used, obtained by self-fertilization of one plant, intercrossing of two plants or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; Hybrid; Onion polymorphism; Genotyping; Primers.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000300274

Desenho de primers degenerados através de bioinformática.
Provedor de dados: 14
Autores: OLIVEIRA, E. M. M.; OLIVEIRA, T. C. de; SOUZA, A. M. de; SANTOS, T. F. DOS.; LIMA, I. S. DE.
bitstream/item/132994/1/CT-208-finalizado.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Primers; Bioinformática.
Ano: 2015
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1028552

Using ancient DNA to unravel taxonomic puzzles: the identity of Deuterodon pedri (Ostariophysi: Characidae)
Provedor de dados: 83
Autores: Silva,Priscilla C.; Malabarba,Maria C.; Malabarba,Luiz R.
ABSTRACT Accurate identification is essential for any study exploring biodiversity. Unfortunately, museum type specimens preserved for more than a hundred years are often not informative enough for precise identification of the species represented by the name-bearing type. The use of ancient DNA can help solve taxonomic problems when name-bearing types no longer have diagnostic morphological features that allow for an accurate identification of the species involved. That is the case for Deuterodon pedri, an endemic species from a small drainage in the rio Doce basin in Minas Gerais, Brazil, for which the type material is in poor condition. Specimens of D. pedri were collected in 1865 by the Thayer Expedition to Brazil and fixed in spirits, enabling them to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lectogenetype; Mini-Barcode; Primers; Rio Doce; Thayer Expedition.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000100224

Amplification of microsatellites in two haemulid species (Haemulon aurolineatum and Haemulon steindachneri)
Provedor de dados: 50
Autores: Pabón Quintero,Paula E.; Tavera,José Julián; Millán-Márquez,Ana María; Acero P,Arturo.
ABSTRACT Genetic-population studies in marine fish have allowed to study patterns of dispersal and connectivity between habitats. One important tool in population genetics is the use of microsatellite molecular markers. Cross-amplification of microsatellite is a method that consists in using primers designed for one species in a different one but phylogenetically related. Because of the importance of genetic studies of populations in artisanal fisheries species, primers were evaluated and designed for the species Haemulon aurolineatum and Haemulon steindachneri. Samples were collected from the artisanal fisheries in Baru-Colombia. Amplification was standardized for 12 microsatellites which ten were polymorphic for H. aurolineatum and nine for H....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haemulidae; Grunts; Primers; Polymorphism; Population genetics.
Ano: 2018
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97612018000100151

Variabilidade genética interespecífica em formigas cortadeiras do gênero Acromyrmex que ocorrem no Estado do Rio Grande do Sul
Provedor de dados: 65
Autores: Grutzmacher,Douglas Daniel; Loeck,Alci Enimar; Oliveira,Antônio Costa; Zimmer,Paulo Dejalma; Malone,Gaspar.
São poucos os taxonomistas que atuam na identificação de formigas cortadeiras. Os métodos morfométricos utilizados nem sempre permitem uma identificação confiável, em função do acentuado polimorfismo dentro do mesmo formigueiro. O objetivo do trabalho foi testar a possibilidade do uso da ferramenta da biologia molecular para auxiliar na identificação das espécies de formigas cortadeiras do gênero Acromyrmex que ocorrem no Estado do Rio Grande do Sul. O estudo foi realizado com seis espécies de formigas do gênero Acromyrmex coletadas em quatro regiões do RS. Utilizaram-se as técnicas RAPD e AFLP, testando-se inicialmente 50 primers, dos quais apenas 13 foram selecionados por terem amplificado com sucesso fragmentos de todas as espécies estudadas. Embora os...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quenquéns; RAPD; AFLP; Primers.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000400001