Consulta: subjectFacets:"Prostate cancer"
Registros recuperados: 26
Data/hora: 29/05/2024 00:56:06

Multi-target siRNA based on DNMT3A/B homologous conserved region influences cell cycle and apoptosis of human prostate cancer cell line TSU-PR1
Provedor de dados: 74
Autores: Du,Yue-feng; Liang,Liang; Shi,Ying; Long,Qing-zhi; Zeng,Jin; Wang,Xin-yang; He,Da-lin.
Abnormal genome hypermethylation participates in the tumorigenesis and development of prostate cancer. Prostate cancer cells highly express DNA methyltransferase 3 (DMNT3) family genes, essential for maintaining genome methylation. In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro investigation of its effects on the proliferation, migration, and invasion of TSU-PR1 prostate cancer cells. The consequential cell-cycle derangement, through DNMT3A/B or only DNMT3B silencing, was partially efficient, without affecting apoptosis. DNMT3A silencing had absolutely no effect on changing TSU-PR1 cell biological behavior. Hence, DNMT3B alone apparently plays a key role in maintaining the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; DNA methylation; DNMT3; RNA interference.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100024

Relationship between XPD, RAD51, and APEX1 DNA repair genotypes and prostate cancer risk in the male population of Rio de Janeiro, Brazil
Provedor de dados: 74
Autores: Cypriano,Ana Sheila; Alves,Gilda; Ornellas,Antonio Augusto; Scheinkman,José; Almeida,Renata; Scherrer,Luciano; Lage,Claudia.
Abstract Susceptibility to cancer ensues in individuals carrying malfunctioning DNA repair mechanisms. The impact of Single Nucleotide Polymorphisms (SNPs) in key DNA repair mechanisms on risk for prostate cancer was investigated in this case-control study. Samples consisted of 110 patients with confirmed prostate cancer and 200 unaffected men, from Rio de Janeiro, Brazil. XPD/Lys751Gln (rs13181), APEX1/Asp148Glu (rs1130409), and RAD51/G135C (rs1801320) SNPs were analyzed by PCR-RFLP. Allelic and genotypic frequencies were calculated and compared by Chi-Square test. The association between SNPs and clinical/epidemiological data was considered significant by Odds Ratio analysis, with IC95% and a p-value≤0.05. Only the XPD/Lys751Gln SNP significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; Single nucleotide polymorphism; XPD; DNA repair; Gene-environment interaction.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500751

Differential expression of the KLK2 and KLK3 genes in peripheral blood and tissues of patients with prostate cancer
Provedor de dados: 74
Autores: Meola,Juliana; Goulart,Luiz R.; Oliveira,Jaqueline D.D.; Neves,Adriana F.; Oliveira Jr.,Waldesse P.; Saraiva,Ana C.M.; Capaneli,Andréia C.; Cardoso,Alexandra M.; Prado,Lindolfo D.; Borba,Sebastião A.; Silva,Heyder D.
We used the multiplex semi-quantitative reverse-transcriptase PCR (RT-PCR) to investigate kallikrein 2 and 3 (KLK2 and KLK3) mRNA levels in prostate tissue from 42 prostate cancer patients, 33 of whom were also assessed for peripheral blood KLK2 expression by qualitative semi-nested RT-PCR. We found that KLK2 was an important tissue biomarker for distinguishing between prostate cancer patients and those with benign prostatic hyperplasia, particularly when KLK2 expression was &gt; 60% of that of the beta2-microglobulin constitutive gene. Patients with an average relative expression value &gt; 0.6 (cutoff value) had an eleven-fold higher chance of having prostate cancer. When one or two tissues samples were evaluated for KLK2 expression using the cutoff...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kallikrein II; Molecular markers; Prostate cancer; PSA; Semi-quantitative RT-PCR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200001

Expression of miR-148b-3p is correlated with overexpression of biomarkers in prostate cancer
Provedor de dados: 74
Autores: Arámbula-Meraz,Eliakym; Bergez-Hernández,Fernando; Leal-León,Emir; Romo-Martínez,Enrique; Picos-Cárdenas,Verónica; Luque-Ortega,Fred; Romero-Quintana,Jose; Alvarez-Arrazola,Marco; García-Magallanes,Noemí.
Abstract Prostate cancer (PCa) is one of the leading causes of death among men. Genes such as PCA3, PSA, and Fra-1 are suggested to serve as potential tools for the detection of PCa, as they are deregulated during this pathology. A similar event occurs with small non-coding RNAs, called miRNAs, specifically miR-195-5p, miR-133a-3p, and miR-148b-3p, which were analyzed in a Chinese population and suggested to be possible candidates for PCa diagnosis. We evaluated the expression levels of three miRNAs and three genes in tissue samples of PCa and benign prostate disease, such as benign prostatic hyperplasia, or prostatitis, in order to determine their potential as candidates for PCa detection. Our results showed a statistically significant overexpression of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene expression; Biomarker; MiRNAs; Correlation; Prostate cancer.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100112

Protective effect of carboxymethyl-glucan (CM-G) against DNA damage in patients with advanced prostate cancer
Provedor de dados: 74
Autores: Magnani,Marciane; Castro-Gomez,Raul Jorge Hernan; Mori,Mateus Prates; Kuasne,Hellen; Gregório,Emerson Pereira; Libos Jr,Farid; Cólus,Ilce Mara de Syllos.
Carboxymethyl-glucan (CM-G) is a soluble derivative from Saccharomyces cerevisiae (1 → 3)(1 → 6)-β-D-glucan. The protective efficiency of CM-G against DNA damage in cells from patients with advanced prostate cancer (PCa), and undergoing Androgen Deprivation Therapy (ADT), was evaluated. DNA damage scores were obtained by the comet assay, both before and after treatment with CM-G. The reduction in DNA damage, ranging from 18% to 87%, with an average of 59%, was not related to the increased number of leukocytes in peripheral blood. The results demonstrate for the first time the protective effect of CM-G against DNA damage in patients with advanced PCa. Among smokers, three presented the highest reduction in DNA damage after treatment with CM-G. There was no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Β-D-glucan; Carboxymethyl-glucan; Prostate cancer.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100023

Lack of detection of human papillomavirus DNA in prostate carcinomas in patients from northeastern Brazil
Provedor de dados: 74
Autores: Araujo-Neto,Ari P.; Ferreira-Fernandes,Hygor; Amaral,Carolina M.M.; Santos,Lina G.; Freitas,Antônio C.; Silva-Neto,Jacinto C.; Rey,Juan A.; Burbano,Rommel R.; Silva,Benedito B. da; Yoshioka,France K.N.; Pinto,Giovanny R.
Abstract Prostate cancer is the second most common cancer among men in western populations, and despite its high mortality, its etiology remains unknown. Inflammatory processes are related to the etiology of various types of tumors, and prostate inflammation, in particular, has been associated with prostate cancer carcinogenesis and progression. Human papillomavirus (HPV) is associated with benign and malignant lesions in the anogenital tract of both females and males. The possible role of HPV in prostate carcinogenesis is a subject of great controversy. In this study, we aimed to examine the prevalence of HPV infections in prostate carcinomas of patients from northeastern Brazil. This study included 104 tissue samples from primary prostate carcinoma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Prostate cancer; Prevalence; HPV PCR detection; Molecular epidemiology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100024

Characterization and in vitro antitumor activity of polymeric nanoparticles loaded with Uncaria tomentosa extract
Provedor de dados: 42
Autores: RIBEIRO,ANA F.; SANTOS,JULIANA F.; MATTOS,RÔMULO R.; BARROS,ELIANE G.O.; NASCIUTTI,LUIZ EURICO; CABRAL,LÚCIO M.; SOUSA,VALERIA P. DE.
Abstract  Uncaria tomentosa (UT) extracts have been shown to have promising anti-tumor activity. We hypothesized that its incorporation into nanostructured systems could improve the anticancer properties. Here, poly-e-caprolactone (PCL) and poly-d,l-lactide-co-glycolide (PLGA) were employed to generate nanoparticles loaded with UT extract in a single emulsion solvent evaporation method. The nanoparticles were characterized by particle size, zeta potential, morphology and entrapment efficiency along with stability and release profiles. The nanoparticles presented entrapment efficiencies above 60% and a mean diameter below 300nm. UT-PCL nanoparticles presented higher entrapment efficiency and mean particle size as well as a slow release rate. The UT-PLGA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanoparticles; Prostate cancer; PLGA; PCL; Anti-tumor; Anti-cancer; Cat’s Claw.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100908

Molecular signature of cancer stem cells isolated from prostate carcinoma and expression of stem markers in different Gleason grades and metastasis
Provedor de dados: 48
Autores: Castellón,Enrique A; Valenzuela,Rodrigo; Lillo,Jorge; Castillo,Viviana; Contreras,Héctor R; Gallegos,Iván; Mercado,Alejandro; Huidobro,Christian.
Prostate cancer (PCa) is the most frequently diagnosed malignancy in men worldwide. Chemotherapy response is very poor and resistance to hormone-based treatments is frequent in advances stages. Recently, tumor-initiating cells or cancer stem cells (CSCs) have been identified in several cancers, including PCa. These cells are thought to be responsible for therapy resistance, relapse and metastasis. In the present work, enriched populations of CSCs were obtained using a mixed procedure that included differential clone-forming ability, sphere growing induction (prostatospheres) and magnetic-associated cell sorting (MACS). Also, stem marker expression was determined in PCa biopsies of different histological grades and metastasis samples. The signature for stem...
Tipo: Journal article
Palavras-chave: Prostate cancer; Cancer stem cell; Spheres cultures.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300011

Role of the phosphatase PP4 in the activation of JNK-1 in prostate carcinoma cell lines PC-3 and LNCaP resulting in increased AP-1 and EGR-1 activity
Provedor de dados: 48
Autores: INOSTROZA,JUAN; SÁENZ,LEONARDO; CALAF,GLORIA; CABELLO,GERTRUDIS; PARRA,EDUARDO.
The specific signaling connections between the mitogen-activated protein kinases (MAPK) such as c-Jun N-terminal kinase (JNK-1) and phosphatases PP4 and M3/6, affecting the family of early nuclear factors, is complex and remains poorly understood. JNK-1 regulates cellular differentiation, apoptosis and stress responsiveness by up-regulating early nuclear factors such as c-Jun, a member of the activating protein (AP-1) family, and the Early Growth Factor (EGR-1). C-Jun, when phosphorylated by c-Jun N-terminal kinase (JNK-1) associates with c-Fos to form the AP-1 transcription factor that activates gene expression. We have investigated the regulation of the JNK-1 kinase by co-transfecting phosphatases PP4 and M3/6 in prostate cancer cell lines PC-3 and...
Tipo: Journal article
Palavras-chave: Prostate cancer; Phosphatase PP4; Kinase JNK-1.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200006

Current evaluation of the clinical utility of Fluoromethylcholine-(18F) PET/CT in Prostate Cancer
Provedor de dados: 52
Autores: Talbot,Jean-Nöel; Chevalme,Yanna-Marina.
This short review is dedicated to the current status of the assessment of a new PET radiopharmaceutical, fluoromethylcholine-(18F) or FCH, which is taken-up by prostate cancer tissue, in contrary to fluorodeoxyglucose-(18F) or FDG. It seems that FCH could become "the FDG of prostate cancer", with the same type of achievements (detection of distant metastases and of occult recurrences, restaging prior to invasive treatments), and the same drawbacks (false negative results in case of small lesions, in particular lymph nodes metastases, and false positive results in case of infection/inflammation, in particular prostatitis). Current evidence is summarised and discussed for each of the potential settings of FCH PET/CT imaging in prostate cancer. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluoromethylcholine-(18F); PET/CT; Prostate cancer; Evaluation of radiopharmaceuticals.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700012

Prostate cancer, treatment modalities and complications: an evaluation of the scientific literature
Provedor de dados: 52
Autores: Santos-Filho,Sebastião David; Missailids,Sotiris; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario.
Prostate (PR) cancer (CA) is one of the most common malignant neoplasms in men all over the world. In general, if prostate cancer (PC) is detected early, treatment usually involves either surgical removal of the prostate or radiotherapy (RT). Hormone Therapy (HT) or chemotherapy (CH) is the preferred treatment for more advanced cases of PC or if CA spreads beyond the PT. A number of complications, such as urinary incontinence (IU) or erectile dysfunction (ED), can be associated with some modalities of treatment of the PC. The aim of this work is to evaluate, in PubMed, the number of publications related with prostate cancer and the main modalities of treatment, as well as some clinical complications. The searches were performed in PubMed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; Radiotherapy; PubMed; Scientific interest.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700009

Association between prostate cancer and schistosomiasis in young patients: a case report and literature review
Provedor de dados: 55
Autores: Bacelar,Albert; Castro,Larissa G.M.C.; Queiroz,Aristides Cheto de; Café,Eduardo.
This case report refers to a 47-year old patient with prostate cancer associated with schistosomiasis mansoni, who was submitted to radical prostatectomy. This is the third report published in the literature with respect to this association, and up to the present time it is still not known whether a cause and effect relationship exists between the two pathologies. The association between schistosomiasis and cancer has been well-documented in bladder cancer; however, there are no data yet proving the association of this disease with prostatic neoplasia. In this report, a third documented case of prostatic adenocarcinoma and schistosomiasis mansoni is described and a literature review is performed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; Schistosomiasis; Prostatectomy; Pathology.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000500014

Construction of a fusion plasmid containing the PSCA gene and cytotoxic T-lymphocyte associated antigen-4 (CTLA-4) and its anti-tumor effect in an animal model of prostate cancer
Provedor de dados: 56
Autores: Mai,T.J.; Ma,R.; Li,Z.; Bi,S.C.
Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) is a negative regulator of T cell activation, which competes with CD28 for B7.1/B7.2 binding, and which has a greater affinity. Fusion of specific antigens to extracellular domain of CTLA4 represents a promising approach to increase the immunogenicity of DNA vaccines. In this study, we evaluated this interesting approach for CTLA4 enhancement on prostate stem cell antigen (PSCA)-specific immune responses and its anti-tumor effects in a prostate cancer mouse model. Consequently, we constructed a DNA vaccine containing the PSCA and the CTLA-4 gene. Vaccination with the CTLA4-fused DNA not only induced a much higher level of anti-PSCA antibody, but also increased PSCA-specific T cell response in mice. To...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; DNA vaccine; PSCA; CTLA-4.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001100601

Effect of the consumption of tomato paste on plasma prostate-specific antigen levels in patients with benign prostate hyperplasia
Provedor de dados: 56
Autores: Edinger,M.S.; Koff,W.J.
The consumption of tomatoes and tomato products has been associated with a reduced risk of prostate cancer. We observed a decrease of 10.77% in prostate-specific antigen (PSA) levels in patients with benign prostate hyperplasia who were submitted to daily ingestion of tomato paste. This was an experimental rather than a controlled study with a sample of 43 men ranging in age from 45 to 75 years, all with histological diagnoses of benign prostate hyperplasia and plasma PSA levels of 4-10 ng/mL. All patients received 50 g of tomato paste once a day for 10 consecutive weeks and PSA levels were analyzed before, during and after the consumption of tomato paste. ANOVA for repeated measures was used to compare PSA levels before, during and after the consumption...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Prostate cancer; Prostate-specific antigen; Tomatoes; Tomato paste; Lycopene.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800014

Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU 145 human prostate cancer cells
Provedor de dados: 56
Autores: Vucic,V.; Isenovic,E.R.; Adzic,M.; Ruzdijic,S.; Radojcic,M.B.
Gamma-irradiation (gamma-IR) is extensively used in the treatment of hormone-resistant prostate carcinoma. The objective of the present study was to investigate the effects of 60Co gamma-IR on the growth, cell cycle arrest and cell death of the human prostate cancer cell line DU 145. The viability of DU 145 cells was measured by the Trypan blue exclusion assay and the 3(4,5-dimethylthiazol-2-yl)-2,5,diphenyltetrazolium bromide test. Bromodeoxyuridine incorporation was used for the determination of cell proliferation. Cell cycle arrest and cell death were analyzed by flow cytometry. Superoxide dismutase (SOD), specifically CuZnSOD and MnSOD protein expression, after 10 Gy gamma-IR, was determined by Western immunoblotting analysis. gamma-IR treatment had a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gamma-irradiation; Prostate cancer; Proliferation; Cell death; Cell cycle arrest; Superoxide dismutase.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200009

Differences in prostate cancer detection between Canadian and Saudi populations
Provedor de dados: 56
Autores: Al-Abdin,O.Z.; Rabah,D.M.; Badr,G.; Kotb,A.; Aprikian,A.
Few studies have addressed racial differences in prostate cancer (PCa) detection between Western and Arabian countries, although PCa has a significantly lower prevalence in Arabic populations compared to Western populations. Therefore, an explanation of this difference is lacking. Serum prostate-specific antigen (PSA) is a valuable marker used to select patients who should undergo prostate biopsies, although the manner in which it is used may require adjustments based on the ethnic population in question. We investigated racial differences in the PCa detection rate between Canadian and Saudi populations. A retrospective analysis was performed of data collected prospectively over 5 consecutive years in urology clinics at the McGill University Health Center...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; Serum; PSA; PSA density; Digital rectal examination.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600539

The role of osteoblasts in regulating hematopoietic stem cell activity and tumor metastasis
Provedor de dados: 56
Autores: Neiva,K.; Sun,Y.-X.; Taichman,R.S.
Bone marrow stromal cells are critical regulators of hematopoiesis. Osteoblasts are part of the stromal cell support system in bone marrow and may be derived from a common precursor. Several studies suggested that osteoblasts regulate hematopoiesis, yet the entire mechanism is not understood. It is clear, however, that both hematopoietic precursors and osteoblasts interact for the production of osteoclasts and the activation of resorption. We observed that hematopoietic stem cells (HSCs) regulate osteoblastic secretion of various growth factors, and that osteoblasts express some soluble factors exclusively in the presence of HSCs. Osteoblasts and hematopoietic cells are closely associated with each other in the bone marrow, suggesting a reciprocal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoblasts; Hematopoietic stem cells; Bone marrow; Stromal-derived factor-1; CXCR4; Prostate cancer.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000001

Rarity of DNA sequence alterations in the promoter region of the human androgen receptor gene
Provedor de dados: 56
Autores: Cabral,D.F.; Santos,A.; Ribeiro,M.L.; Mesquita,J.C.; Carvalho-Salles,A.B.; Hackel,C.
The human androgen receptor (AR) gene promoter lies in a GC-rich region containing two principal sites of transcription initiation and a putative Sp1 protein-binding site, without typical "TATA" and "CAAT" boxes. It has been suggested that mutations within the 5'untranslated region (5'UTR) may contribute to the development of prostate cancer by changing the rates of gene transcription and/or translation. In order to investigate this question, the aim of the present study was to search for the presence of mutations or polymorphisms at the AR-5'UTR in 92 prostate cancer patients, where histological diagnosis of adenocarcinoma was established in specimens obtained from transurethral resection or after prostatectomy. The AR-5'UTR was amplified by PCR from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human androgen receptor gene; Promoter region; Prostate cancer; 5'Untranslated region; DNA sequence alterations.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200004

Cyclin D1 expression in prostate carcinoma
Provedor de dados: 56
Autores: Pereira,R.A.; Ravinal,R.C.; Costa,R.S.; Lima,M.S.; Tucci,S.; Muglia,V.F.; Dos Reis,R.B.; Silva,G.E.B.
The purpose of this study was to investigate the relationship between cyclin D1 expression and clinicopathological parameters in patients with prostate carcinoma. We assessed cyclin D1 expression by conventional immunohistochemistry in 85 patients who underwent radical prostatectomy for prostate carcinoma and 10 normal prostate tissue samples retrieved from autopsies. We measured nuclear immunostaining in the entire tumor area and based the results on the percentage of positive tumor cells. The preoperative prostate-specific antigen (PSA) level was 8.68±5.16 ng/mL (mean±SD). Cyclin D1 staining was positive (cyclin D1 expression in &gt;5% of tumor cells) in 64 cases (75.4%) and negative (cyclin D1 expression in ≤5% of tumor cells) in 21 cases (including 15...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; Cyclin D1; Immunohistochemistry.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600515

Construction of a recombinant adenovirus co-expressing truncated human prostate-specific membrane antigen and mouse 4-1BBL genes and its effect on dendritic cells
Provedor de dados: 56
Autores: Weng,Xiaodong; Kuang,Youlin; Liu,Xiuheng; Chen,Zhiyuan; Zhu,Hengcheng; Chen,Hui; Jiang,Botao; Shen,Hao.
Our aim was to construct a recombinant adenovirus co-expressing truncated human prostate-specific membrane antigen (tPSMA) and mouse 4-1BBL genes and to determine its effect on dendritic cells (DCs) generated from bone marrow suspensions harvested from C57BL/6 mice for which the effect of 4-1BBL on DCs is not clear, especially during DCs processing tumor-associated antigen. Replication deficient adenovirus AdMaxTM Expression System was used to construct recombinant adenovirus Ad-tPSMA-internal ribosome entry site-mouse 4-1BBL (Ad-tPSMA-IRES-m4-1BBL) and Ad-enhanced green fluorescent protein. Day 7 proliferating DC aggregates generated from C57BL/6 mice were collected as immature DCs and further mature DCs were obtained by lipopolysaccharide activated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; PSMA; Co-stimulatory molecule; 4-1BBL; Adenovirus; Dendritic cells.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300002

Obesity inversely correlates with prostate-specific antigen levels in a population with normal screening results of prostate cancer in northwestern China
Provedor de dados: 56
Autores: Zhang,J.; Ma,M.; Nan,X.; Sheng,B.
Serum prostate-specific antigen (PSA) is a diagnostic biomarker of prostate cancer and is possibly associated with obesity. This study aimed to explore the relationships between obesity indicators [body mass index (BMI) and waist circumference (WC)] with PSA in Chinese men. A cross-sectional study of men aged 30-85 years undergoing prostate cancer screening was conducted from August 2008 to July 2013 in Xi'an, China. Data were obtained from clinical reports, condition was recorded based on self-report including demographics, weight, height, and WC (&gt;90 cm=obese). Fasting blood glucose (FBG) and prostate volume (PV) were assessed clinically. Patients were grouped by BMI (normal=22.9, overweight=23-27.4, obese≥27.5 kg/m2). PSA parameters of density...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate-specific antigen; Body mass index; Obesity; Prostate cancer; Screening.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800704

LncRNA HULC mediates radioresistance via autophagy in prostate cancer cells
Provedor de dados: 56
Autores: Chen,Changxuan; Wang,Kaizhen; Wang,Qian; Wang,Xin.
Prostate cancer (PCa) is the second leading cause of cancer death in men. Irradiation is one of the available options for treatment of PCa, however, approximately 10–45% of PCa are resistant to irradiation. We aimed to explore the role of long non-coding RNA highly upregulated in liver cancer (HULC) in the sensitivity of PCa cells to irradiation. Survival rate, cell apoptosis, cycle, expressions of related proteins, and caspase-3 activity were assessed to explore the effects of HULC on sensitivity of PCa cells to irradiation. Expression of HULC in DU-145, PC3, LNCaP, and RWPE-1 cells was determined and the influence of HULC on DU-145 cells was explored. Then, PC3 cells aberrantly expressing HULC were implanted into NOD-SCID mice for tumor xenograft study....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; LncRNA HULC; Irradiation; Autophagy; MTOR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600611

Inhibition of cytohesin-1 by siRNA leads to reduced IGFR signaling in prostate cancer
Provedor de dados: 56
Autores: Weizhong,Zhang; Shuohui,Gao; Hanjiao,Qin; Yuhong,Man; Xiaohua,Yang; Jian,Cao; Lisen,Li.
To explore how cytohesin-1 (CYTH-1) small interfering RNA (siRNA) influences the insulin-like growth factor receptor (IGFR)-associated signal transduction in prostate cancer, we transfected human prostate cancer PC-3 cell lines with liposome-encapsulatedCYTH-1 siRNA in serum-free medium and exposed the cells to 100 nM IGF-1. The mRNA and protein levels of the signal molecules involved in the IGFR signaling pathways were determined by real-time PCR and detected by Western blotting. The relative mRNA levels of CYTH-1, c-Myc, cyclinD1 and IGF-1R (CYTH-1 siRNA group vs scrambled siRNA group) were 0.26 vs 0.97, 0.34 vs 1.06, 0.10 vs 0.95, and 0.27 vs 0.41 (P &lt; 0.05 for all), respectively. The relative protein levels of CYTH-1, pIGF-1R, pIRS1, pAkt1, pErk1,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytohesins; Insulin-like growth factor 1 receptor; Prostate cancer; Signal transduction; SiRNA interference.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700005

Allelic frequencies of six polymorphic markers for risk of prostate cancer
Provedor de dados: 56
Autores: Ribeiro,M.L.; Santos,A.; Carvalho-Salles,A.B.; Hackel,C.
The aim of the present study was to evaluate the distribution of polymorphisms for the androgen receptor (AR) (CAG, StuI, GGN), SRD5A2 (Ala49Thr, Val89Leu) and CYP17 (MspA1) genes that are considered to be relevant for risk of prostate cancer. We studied 200 individuals from two cities in the State of São Paulo, by PCR, PCR-RFLP and ASOH techniques. The allelic frequencies of the autosomal markers and the StuI polymorphism of the AR gene were very similar to those described in most North American and European populations. In relation to the CAG and GGN number of repeats, the study subjects had smaller repeat lengths (mean of 20.65 and 22.38, respectively) than those described in North American, European and Chinese populations. In the present study, 30.5%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; Polymorphisms; AR; SRD5A2; CYP17.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200009

RNAi-mediated knockdown of pituitary tumor- transforming gene-1 (PTTG1) suppresses the proliferation and invasive potential of PC3 human prostate cancer cells
Provedor de dados: 56
Autores: Huang,S.Q.; Liao,Q.J.; Wang,X.W.; Xin,D.Q.; Chen,S.X.; Wu,Q.J.; Ye,G.
Pituitary tumor-transforming gene-1 (PTTG1) is a proto-oncogene that promotes tumorigenesis and metastasis in numerous cell types and is overexpressed in a variety of human tumors. We have demonstrated that PTTG1 expression was up-regulated in both human prostate cancer specimens and prostate cancer cell lines. For a more direct assessment of the function of PTTG1 in prostate tumorigenesis, RNAi-mediated knockdown was used to selectively decrease PTTG1 expression in PC3 human prostate tumor cells. After three weeks of selection, colonies stably transfected with PTTG1-targeted RNAi (the knockdown PC3 cell line) or empty vector (the control PC3 cell line) were selected and expanded to investigate the role of PTTG1 expression in PC3 cell growth and invasion....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PTTG1; Prostate cancer; Cell proliferation; Transwell invasion assay; Anticancer drug resistance.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100001

Preparation and physicochemical characterization of prazosin conjugated PLGA nanoparticles for drug delivery of flutamide
Provedor de dados: 60
Autores: Fattahi,Ali; Ghiasi,Mansoureh; Mohammadi,Pardis; Hosseinzadeh,Leila; Adibkia,Khosro; Mohammadi,Ghobad.
In the current work, a sustained drug delivery system of flutamide (FLT) was developed using Poly(D,L-lactide-co-glycolide) (PLGA) decorated bypoly(ethylene glycol) (PEG) grafted prazosin (PLGA-PEG-Praz) as a targeting moiety. In a multi-step reaction, PLGA was linked to PEG and prazosin. The structure of the synthesized polymers was confirmed by FTIR and 1H-NMR. Flutamide-loaded nanoparticles were prepared by quasi-emulsion solvent diffusion technique. The nanoparticles were evaluated for size, zeta potential, polydispersity index, drug crystallinity, loading efficiency, and release properties. Also, the physicochemical properties of the nanoparticles were analyzed using Scanning Electron Microscopy (SEM), Differential Scanning Calorimetry, and Powder...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanoparticle; PLGA; Flutamide; Prazosin; Prostate cancer; Drug delivery.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400609