Consulta: subjectFacets:"Protease"
Registros recuperados: 53
Data/hora: 09/06/2024 14:39:38

Potential application of protease isolated from Pseudomonas aeruginosa PD100
Provedor de dados: 69
Autores: Najafi,Mohsen Fathi; Deobagkar,Dileep; Deobagkar,Deepti.
A protease isolated from Pseudomonas aeruginosa PD100 could act in the presence of SDS and Tween 80. This protease could be useful for degradation of protein in the presence of solvent, dehairing of cow skin and degradation of natural proteins. The immobilized protease showed 15-20% increases in temperature stability and the entrapped enzyme retained 83% of its initial activity after six cycles. With respect to properties of the enzyme and its capability for degradation of different protein sources, this protease finds potential application for waste treatment, used in detergents and leather industry
Tipo: Journal article
Palavras-chave: Blood clot; Protease; Pseudomonas aeruginosa PD100; SDS; Solvent.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200009

Production of an extracellular keratinase from Chryseobacterium sp. growing on raw feathers
Provedor de dados: 69
Autores: Brandelli,Adriano; Riffel,Alessandro.
The strain Chryseobacterium sp. kr6 shown to be useful for biotechnological purposes such as hydrolysis of poultry feathers and de-hairing of bovine pelts. The effect of temperature, initial pH and media composition on protease production by this keratinolytic strain was studied. The enzyme was produced between 25 and 37ºC, with maximum activity and yield at 30ºC. When protease production was tested in media with different initial pH, maximum activity was observed when cultivation was carried out at 30ºC and initial pH ranging from 6.0 to 8.0. Higher activity was observed when feathers or feather meal were used as growth substrates, followed by soybean meal. The addition of carbohydrates or surfactants to feather broth resulted in decrease in keratinolytic...
Tipo: Journal article
Palavras-chave: Bacteria; Enzyme; Keratin; Protease.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100007

Looking for exceptions on knowledge rules induced from HIV cleavage data set
Provedor de dados: 74
Autores: Prati,Ronaldo Cristiano; Monard,Maria Carolina; Carvalho,André C. P. L. F. de.
The aim of data mining is to find useful knowledge inout of databases. In order to extract such knowledge, several methods can be used, among them machine learning (ML) algorithms. In this work we focus on ML algorithms that express the extracted knowledge in a symbolic form, such as rules. This representation may allow us to ''explain'' the data. Rule learning algorithms are mainly designed to induce classification rules that can predict new cases with high accuracy. However, these sorts of rules generally express common sense knowledge, resulting in many interesting and useful rules not being discovered. Furthermore, the domain independent biases, especially those related to the language used to express the induced knowledge, could induce rules that are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Data mining; Exceptions; HIV; Protease.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400027

NS3 protease from flavivirus as a target for designing antiviral inhibitors against dengue virus
Provedor de dados: 74
Autores: Natarajan,Satheesh.
The development of novel therapeutic agents is essential for combating the increasing number of cases of dengue fever in endemic countries and among a large number of travelers from non-endemic countries. The dengue virus has three structural proteins and seven non-structural (NS) proteins. NS3 is a multifunctional protein with an N-terminal protease domain (NS3pro) that is responsible for proteolytic processing of the viral polyprotein, and a C-terminal region that contains an RNA triphosphatase, RNA helicase and RNA-stimulated NTPase domain that are essential for RNA replication. The serine protease domain of NS3 plays a central role in the replicative cycle of dengue virus. This review discusses the recent structural and biological studies on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antiviral inhibitor; Drug discovery; Multifunctional protein; NS3; Protease.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200002

Boundaries in metagenomic screenings using lacZα-based vectors
Provedor de dados: 74
Autores: Alves,Luana de Fátima; Borelli,Tiago Cabral; Westmann,Cauã Antunes; Silva-Rocha,Rafael; Guazzaroni,María-Eugenia.
Abstract Metagenomics approaches have been of high relevance for providing enzymes used in diverse industrial applications. In the current study, we have focused on the prospection of protease and glycosyl hydrolase activities from a soil sample by using the lacZα -based plasmid pSEVA232. For this, we used a functional screen based on skimmed milk agar and a pH indicator dye for detection of both enzymes, as previously reported in literature. Although we effectively identified positive clones in the screenings, subsequent experiments revealed that this phenotype was not because of the hydrolytic activity encoded in the metagenomic fragments, but rather due to the insertion of small metagenomic DNA fragments in frame within the coding region of the lacZ...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Functional metagenomics; Protease; Glycosyl hydrolase; False positive clones.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100803

Efeito do uso de proteanase na dieta de frangos de corte sobre rendimento de carcaça.
Provedor de dados: 14
Autores: AVILA, V. S. de; GOPINGER, E.; BOTTCHER, A.; RIGO, P. P.; KRABBE, E. L.
bitstream/item/166818/1/final8658.pdf
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Produção industrial; Frango de corte; Carcaça; Peso; Rendimento; Nutrição animal; Dieta; Enzima; Protease.
Ano: 2017
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1079914

Proteases e inibidores de proteases na defesa de plantas contra pragas.
Provedor de dados: 14
Autores: TREMACOLDI, C. R.
bitstream/item/28164/1/Doc353.pdf
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Doença de planta; Microrganismo; Controle biológico; Fungo; Praga; Defesa vegetal; Protease; Fisiologia vegetal.
Ano: 2009
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/662441

Técnica Zimográfica como método para monitoramento da Artrite Encefalite Caprina (CAE).
Provedor de dados: 14
Autores: ELOY, A. M. X.; BEZERRA JUNIOR, R. Q.; PINHEIRO, R. R.; ANDRIOLI, A.
A técnica zimográfica é uma ferramenta de fácil execução, de baixo custo e de acurácia comprovada na identificação das formas latentes e ativas das MMPs, mostrando-se eficaz na identificação do grau de atividade do sistema imune inato frente ao vírus da Artrite Encefalite Caprina (CAE). Portanto, poderá vir a ser utilizada para monitorar rebanhos sadios, avaliar a resistência dos animais frente à infecção e colaborar com programas de controle da doença.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Artrite Encefalite Caprina; CAE; CAEV; Metaloproteinase; Técnica zimográfica; Proteases.; Caprino; Doença animal; Protease; Goats; Proteolysis; Metalloproteins; Caprine arthritis encephalitis virus.
Ano: 2015
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1051850

Calpain and cathepsin activities in post mortem fish and meat muscles
Provedor de dados: 5
Autores: Cheret, Romuald; Delbarre Ladrat, Christine; De Lamballerie Anton, Marie; Verrez-bagnis, Veronique.
Post mortem tenderization is one of the most unfavourable quality changes in fish muscle and this contrasts with muscle of mammalian meats. The tenderization can be partly attributed to the acid lysosomal cathepsins and cytosolic neutral calcium-activated calpains. In this study, these proteases from fish and bovine muscles were quantified and compared. The cathepsin B and L activities were in more important amounts in sea bass white muscle than in bovine muscle. On the other hand, cathepsin D activity was 1.4 times higher in meat that in fish muscle, while cathepsin H was negligible in both muscles. Calpain activities were similar in both types of muscle. Moreover, calpastatin (calpain endogenous inhibitor) level is 3.9 times higher in sea bass white...
Tipo: Text
Palavras-chave: Meat; Fish; Protease; Cathepsin; Calpain.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3646.pdf

De l'utilisation de protéases industrielles pour la production de sauces de poisson
Provedor de dados: 5
Autores: Le, Minh Chau.
Fish sauces are traditionally used in most Asian countries. Whatever the process, the principle is the same i.e. a fish autolysis in saline media followed by a fermentation step. Not less than 18 months are needed to obtain a brown liquid rich in salt and amino acids. This PhD work aims to determine if an addition of proteases can speed up the process while keeping the nutritional and sensorial properties. The first chapter presents the characteristics of fish sauces and the key parameters of the processes used. In the second chapter, several industrial proteases are compared each other for their capacity to liquefy the fishes in hyper saline conditions. Kinetics of proteolysis and biochemical characteristics of the resulting hydrolysates are established...
Tipo: Text
Palavras-chave: Sauce de poisson; Hydrolyse; Enzyme; Protéase; Sardine; Anchois; Fish sauce; Hydrolysis; Enzyme; Protease; Sardine; Anchovy.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00259/36997/35488.pdf

Subproteome of Lachesis muta rhombeata venom and preliminary studies on LmrSP-4, a novel snake venom serine proteinase
Provedor de dados: 80
Autores: Wiezel,Gisele A; Bordon,Karla CF; Silva,Ronivaldo R; Gomes,Mário SR; Cabral,Hamilton; Rodrigues,Veridiana M; Ueberheide,Beatrix; Arantes,Eliane C.
Abstract  Background: Lachesis muta rhombeata is one of the venomous snakes of medical importance in Brazil whose envenoming is characterized by local and systemic effects which may produce even shock and death. Its venom is mainly comprised of serine and metalloproteinases, phospholipases A2 and bradykinin-potentiating peptides. Based on a previously reported fractionation of L. m. rhombeata venom (LmrV), we decided to perform a subproteome analysis of its major fraction and investigated a novel component present in this venom.  Methods: LmrV was fractionated through molecular exclusion chromatography and the main fraction (S5) was submitted to fibrinogenolytic activity assay and fractionated by reversed-phase chromatography. The N-terminal sequences of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bushmaster; Snake venom; SVSP; Kallikrein-like; Plasminogen activator; Kininogenase; Lectin; Protease; Envenomation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100307

Enzimas digestivas exógenas na alimentação de juvenis de tambaqui
Provedor de dados: 86
Autores: Nunes,Érica da Silva Santiago; Cavero,Bruno Adan Sagratzki; Pereira-Filho,Manoel; Roubach,Rodrigo.
O objetivo deste trabalho foi avaliar o efeito da adição na ração das enzimas digestivas exógenas amilase, lipase e protease sobre o desempenho de tambaqui, em três experimentos independentes. Em cada experimento foi avaliado o efeito da inclusão de uma enzima na ração em diferentes porcentagens: 0,0, 0,05, 0,1 e 0,2%, cada uma com quatro repetições, com 28 dias de duração. Foram realizadas biometrias do peso inicial, para homogeneização da amostra, e do peso final a fim de verificar o efeito dos tratamentos sobre o desempenho dos peixes. Em cada unidade experimental foram estocados dez peixes, alimentados duas vezes ao dia (8 e 14h) até a saciedade aparente, com ração comercial extrusada com 28% de proteína bruta, triturada, adicionada de enzima e depois...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colossoma macropomum; Piscicultura; Nutrição de peixes; Amilase; Lipase; Protease.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000100019

Enzimas exógenas no desempenho e na digestibilidade ileal de nutrientes em frangos de corte
Provedor de dados: 86
Autores: Barbosa,Nei André Arruda; Sakomura,Nilva Kazue; Fernandes,João Batista Kochenborger; Dourado,Leilane Rocha Barros.
O objetivo deste trabalho foi avaliar o efeito da combinação de fitase e do complexo amilase, protease e xilanase, em dietas de milho e soja, formuladas com redução e sem redução dos níveis de energia, cálcio e fósforo sobre o desempenho e a digestibilidade ileal de nutrientes, em frangos de corte. Foi utilizado o delineamento inteiramente casualizado, com esquema fatorial 2x2, com duas dietas-controle: com redução e sem redução dos nutrientes, e duas suplementações das enzimas (sem adição e com adição de fitase e complexo enzimático), com dez repetições de 40 aves. A digesta ileal foi coletada aos 43 dias de idade, para determinação da energia digestível e dos coeficientes de digestibilidade da proteína bruta, matéria seca, cálcio e fósforo. O desempenho...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amilase; Energia; Fitase; Fósforo; Protease.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000600012

Atividade proteolítica e crescimento de matrinxã em natação sustentada e alimentado com dois níveis de proteína
Provedor de dados: 86
Autores: Arbeláez-Rojas,Gustavo Alberto; Inoue,Luís Antônio Kioshi Aoki; Moraes,Gilberto.
O objetivo deste trabalho foi avaliar o efeito da natação sustentada sobre a atividade digestiva proteolítica e o crescimento de juvenis de matrinxã (Brycon amazonicus), alimentados com dois níveis de proteína. Foram utilizados 240 peixes, tratados com 28 ou 38% de proteína bruta (PB), durante 60 dias, em duas situações distintas: em natação sustentada à velocidade de uma vez e meia o comprimento corporal por segundo, e em sistema convencional de cultivo, sem movimento forçado. Os peixes em natação sustentada e alimentados com 28% de PB apresentaram melhor desempenho, expresso como maior crescimento, alta taxa de crescimento específico, maior ganho de peso e melhor eficiência alimentar. A natação sustentada proporcionou aumento significativo da atividade...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brycon amazonicus; Deposição proteica; Enzima digestiva; Exercício físico; Protease; Proteína corporal.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011001100014

Hidrólise enzimática de fibras de cotilédones de soja e caracterização das frações sólidas e solúveis
Provedor de dados: 86
Autores: Seibel,Neusa Fátima; Beléia,Adelaide Del Pino.
O objetivo deste trabalho foi determinar as melhores condições de hidrólise enzimática de fibras alimentares de cotilédones de soja, original (FAO) e micronizada (FAM), e caracterizar os hidrolisados sólidos e solúveis. As amostras foram hidrolisadas com carboidrase (200 µL g-1, durante 12 horas, a 30ºC) ou com protease (150 µL g-1, durante 5 horas, a 55ºC). A fração sólida das amostras tratadas com carboidrase teve redução de 73% dos carboidratos e de 50% dos ácidos urônicos iniciais; houve aumento da concentração de proteínas e aumento da solubilidade e volume de intumescimento comparado com o material não hidrolisado. Proteínas de reserva da soja - beta-conglicinina e glicinina - foram extraídas das fibras alimentares não hidrolisadas e identificadas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Carboidrase; Eletroforese; Fibra alimentar; Protease.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009001000018

Isolamento e seleção de fungos filamentosos do solo de sistemas agroflorestais do Município de Bom Jardim (PE) com base na capacidade de produção de enzimas hidrolíticas
Provedor de dados: 88
Autores: Silva,Dianny Carolyne Vasconcelos da; Tiago,Patricia Vieira; Mattos,Jorge Luiz Schirmer de; Paiva,Laura Mesquita; Souza-Motta,Cristina Maria de.
O presente trabalho teve por objetivo isolar e identificar fungos de solo de sistemas agroflorestais e avaliar a atividade enzimática destes fungos. Estes foram isolados pela técnica de diluição sucessiva e avaliados quanto à capacidade de degradar amido, celulose e caseína. Foram identificadas 11 espécies de fungos anamorfos (Hyphomycetes) distribuídos em cinco gêneros: Aspergillus, Cladosporium, Curvularia, Fusarium e Penicillium. As espécies estudadas não apresentaram atividade amilolítica satisfatória. Cladosporium cladosporioides (Fres.) De Vries e Penicillium chrysogenum Thom apresentaram maior índice de relação enzimática (IRE) para protease e celulase. Entre as espécies estudadas seis apresentaram maior Índice de Relação Enzimática (IRE) para...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Amilase; Celulase; Protease.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042011000400014

Corn and soybean meal metabolizable energy with the addition of exogenous enzymes for poultry
Provedor de dados: 89
Autores: Dourado,LRB; Sakomura,NK; Barbosa,NAA; Bonato,MA; Kawuauchi,IM; Fernandes,JBK; Costa,FGP.
Two metabolism assays were carried out to determine corn and soybean meal metabolizable energy when enzymes were added. In the first trial, 35 cockerels per studied feedstuff (corn and soybean meal) were distributed in a completely randomized experimental design with four treatments of seven replicates of one bird each. The evaluated treatments were: ingredient (corn and soybean meal) with no enzyme addition, with the addition of an enzyme complex (xylanase, amylase, protease - XAP), xylanase, or phytase. Precise feeding method was used to determine true metabolizable energy corrected for nitrogen balance (TMEn). The use of enzymes did not result in any differences (p&gt;0.05) in soybean meal TMEn, but phytase improved corn TMEn in 2.3% (p=0.004). In the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amylase; Forced feeding; Phytase; Protease; Xylanase.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000100008

Inclusion of exogenous enzymes to feathers during processing on the digestible energy content of feather meal for adult dogs
Provedor de dados: 96
Autores: Pacheco,Gabriel Faria Estivallet; Pezzali,Júlia Guazzelli; Kessler,Alexandre de Mello; Trevizan,Luciano.
ABSTRACT This study was conducted to determine the coefficient of total tract apparent digestibility (CTTAD) of nutrients and gross energy (GE), metabolizable energy (ME), coefficient of metabolizability of gross energy (CM of GE), and fecal characteristics of dogs fed diets with two levels of feather meal, with or without addition of an enzyme blend containing lipase and protease activity. Ten adult Beagle dogs (aged between 1 and 2 years and weighing 10.2±1.4 kg) were arranged in a 5 × 3 incomplete Latin square design with five treatments and three periods. The extruded basal diet was provided in equal amounts to all dogs (220 g/d), and 7.5 and 15% of hydrolyzed feather meal without enzymes (HFM) or HFM processed with addition of the enzyme blend (HFM...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: By-product; Digestibility; Lipase; Protease; Protein source.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016000600288

Utilização de enzimas exógenas em dietas com diferentes fontes e níveis de proteína para leitões na fase de creche
Provedor de dados: 96
Autores: Teixeira,Alexandre de Oliveira; Lopes,Darci Clementino; Ferreira,Vanusa Patrícia de Araújo; Pena,Sérgio Miranda; Nogueira,Eduardo Terra; Moreira,José Aparecido; Bünzen,Silvano; Nery,Lidson Ramos.
Dois experimentos foram conduzidos para avaliar a inclusão de enzimas exógenas em dietas com diferentes níveis e fontes de proteína para leitões. No experimento I, 80 leitões dos 21 aos 51 dias de idade, desmamados aos 14 dias de idade, foram distribuídos em blocos casualizados, em esquema fatorial 2x2x2 (dois níveis de proteína; com ou sem farinha de carne e ossos; com ou sem enzima), com cinco repetições e dois leitões/baia, e alimentados com dietas à base de milho (M), farelo de soja (FS) e soro de leite em pó (SLP). No experimento II, 40 leitões dos 28 aos 45 dias de idade, desmamados aos 21 dias de idade, foram distribuídos em blocos casualizados, com quatro tratamentos, cinco repetições e dois animais/baia, e alimentados com uma dieta controle à base...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amilase; Celulase; Desmame; Farinha de carne e ossos; Protease; Suínos.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982005000300023

Efeito da adição de enzimas em dietas de frangos de corte à base de milho e farelo de soja sobre a digestibilidade ileal de nutrientes
Provedor de dados: 96
Autores: Tejedor,Anel Atencio; Albino,Luiz Fernando Teixeira; Rostagno,Horacio Santiago; Lima,Cristina Amorim Ribeiro de; Vieites,Flávio Medeiros.
Dois experimentos, utilizando pintos de corte Avian Farm, no período de 8 a 18 dias de idade, foram realizados para se avaliar o efeito de enzimas microbianas sobre os coeficientes de digestibilidade (CD) ileal aparente da matéria seca (MS), da proteína bruta (PB), da energia bruta (EB), do fósforo (P) e do cálcio (Ca) de dietas à base de milho e farelo de soja com diferentes níveis de Ca e P disponível (Pd). Óxido crômico (0,5%) foi adicionado às dietas a fim de se estimar o fator de indigestibilidade. Todas as aves foram abatidas aos 19 dias, para a coleta da digesta. O primeiro experimento foi realizado em arranjo fatorial 2 x 2, com seis repetições contendo 10 aves. As dietas foram formuladas contendo dois níveis de Ca e Pd normal (1%Ca/0,45%Pd) e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amilase; Celulase; Fitase; Digestibilidade; Frangos de corte; Protease.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982001000300028

VCP1 PROTEASE DETECTION IN Pochonia chlamydosporia var. CATENULATA STRAIN IMI SD 187
Provedor de dados: 100
Autores: Peteira-Delgado,B; Hidalgo-Díaz,L.
The mode of action of Pochonia as a biological control agent is the penetration by means of the hyphae into the target nematode eggs. The egg shell of nematodes has an outer vitelline membrane composed mainly by proteins. Thus, hyphal penetration is the result of a physic pressure and the specific hydrolytic activity of some enzymes such as proteases and chitinases. The most important protease studied in Pochonia chlamydosporia var. chlamydosporia is VCP1. The aim of this work was to detect and characterize the protease VCP1 in a strain of P. chlamydosporia var. catenulata, a potential biological control agent for root-knot nematodes. The specific activity of this protease was tested on different culture media supplemented with protein inductors using...
Tipo: Journal article
Palavras-chave: Nematode parasites; RFLP; Enzymes; Protease.
Ano: 2009
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522009000300006

Efeito do Soil-borne wheat mosaic virus sobre o metabolismo de cinco genótipos de trigo com diferentes níveis de resistência à doença
Provedor de dados: 102
Autores: Souza,Rocheli de; Schons,Jurema; Brammer,Sandra P.; Prestes,Ariano M.; Scheeren,Pedro L.; Nicolini-Teixeira,Fernanda; Cecchetti,Dileta; Lanzarini,Ariane C.
Com o objetivo de conhecer as alterações metabólicas promovidas pelo Soil-borne wheat mosaic virus (SBWMV), um dos vírus economicamente mais importantes da cultura do trigo (Triticum aestivum), foram analisados os níveis de proteínas solúveis e determinadas as atividades da peroxidase e da protease em quatro cultivares (BRS Guabiju, BRS 194, BRS 179, BR 23) e uma linhagem (PF 980524) de trigo com diferentes níveis de resistência ao vírus. Os dados obtidos foram submetidos à análise de variância, comparando-se as médias, pelo Teste de Duncan a 5%. Os níveis de proteínas solúveis foram mais elevados nas plantas sem sintomas, enquanto que as atividades da peroxidase e da protease foram maiores em plantas com sintoma de mosaico do que em plantas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proteínas solúveis; Peroxidase; Protease; Furovirus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400010

Inibidor de tripsina em raízes de Eucalyptus urophylla
Provedor de dados: 102
Autores: Tremacoldi,Célia R.; Pascholati,Sérgio F.
As raízes de eucalipto (Eucalyptus urophylla) podem estar associadas a fungos como Pisolithus tinctorius, formando uma simbiose conhecida como ectomicorriza, mas também podem estar colonizadas por fungos patogênicos, como Rhizoctonia solani, agente causal do tombamento de plantas em viveiros. O objetivo deste trabalho foi verificar a presença de atividade inibitória de tripsina, uma serino-protease, em raízes de E. urophylla e a atividade de tripsina em filtrados desses fungos. Alíquotas de extrato protéico bruto de raízes de E. urophylla e frações protéicas parcialmente purificadas por cromatografia de exclusão molecular, do tipo Sephacryl S-100-HR, foram testadas para atividade inibitória de tripsina. Proteínas do extrato ou das frações, quando incubadas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inibidor de protease; Protease; Ectomicorriza; Rhizoctonia solani.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000200003

Seleção de leveduras da Região Amazônica para produção de protease extracelular
Provedor de dados: 36
Autores: Neves,Kilma Cristiane Silva; Porto,Ana Lúcia Figueiredo; Teixeira,Maria Francisca Simas.
O objetivo deste trabalho foi identificar 50 leveduras isoladas a partir de diferentes substratos da Região Amazônica e selecionar uma espécie de maior atividade proteolítica. Entre as 26 espécies identificadas predominaram Candida aquatica (12%) e Candida tropicalis (10%). A fermentação submersa foi realizada em Extrato de Malte suplementado com gelatina 1%, Candida intermedia foi a que expressou maior atividade proteolítica, halo = 25 mm e 273 U/mL, crescimento máximo a 30 ºC, viabilidade celular 6,2 x 10(6) UFC, em 48 horas, não demonstrou características de patogenicidade e a melhor densidade do inóculo foi 3%, utilizando-se culturas com 24 horas de crescimento em ágar Malte.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leveduras; Região Amazônica; Protease.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672006000300002

Proteolytic activities of bacteria, yeasts and filamentous fungi isolated from coffee fruit (Coffea arabica L.) - doi: 10.4025/actasciagron.v33i3.6734
Provedor de dados: 1
Autores: Rodarte, Mirian Pereira; Ufla; Dias, Disney Ribeiro; UNILAVRAS - Centro Universitário de Lavras; Vilela, Danielle Marques; Ufla; Schwan, Rosane Freitas; Ufla.
One hundred forty-four microorganisms previously isolated from coffee fruit (Coffea Arabica) were grown on casein agar to evaluate their proteolytic activities. Fifty percent of filamentous fungi, 52.5% of bacteria and 2.6% of yeasts were able to secrete proteases. Positive isolates were further examined in liquid culture for their protease activities by hydrolysis of casein at different pH values (5.0, 7.0 and 9.0) at 30 oC. Bacillus megaterium, B. subtilis, Enterobacter agglomerans, Kurthia sp, Pseudomonas paucimobilis and Tatumella ptyseos demonstrated the highest proteolytic activities at pH 9.0. One yeast isolate, Citeromyces matritensis, had a proteolytic activity of 2.40 U at pH 5.0. Aspergillus dimorphicus, A. ochraceus, Fusarium moniliforme, F....
Tipo: Pesquisa de campo; Laboratório
Palavras-chave: 5.01.02.04-4 microbial enzyme; Protease; Coffee fermentation Microbiologia Agrícola microbial enzyme; Protease; Coffee fermentation.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6734

The ubiquitous gp63-like metalloprotease from lower trypanosomatids: in the search for a function
Provedor de dados: 42
Autores: Santos,André L.S.; Branquinha,Marta H.; D'Avila-Levy,Claudia M.
Plant and insect trypanosomatids constitute the "lower trypanosomatids", which have been used routinely as laboratory models for biochemical and molecular studies because they are easily cultured under axenic conditions, and they contain homologues of virulence factors from the classic human trypanosomatid pathogens. Among the molecular factors that contribute to Leishmania spp. virulence and pathogenesis, the major surface protease, alternatively called MSP, PSP, leishmanolysin, EC 3.4.24.36 and gp63, is the most abundant surface protein of Leishmania promastigotes. A myriad of functions have been described for the gp63 from Leishmania spp. when the metacyclic promastigote is inside the mammalian host. However, less is known about the functions performed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosomatidae; Lower trypanosomatids; Gp63; Invertebrate host; Adhesion; Nutrition; Protease.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000400006

Schistosome serine protease inhibitors: parasite defense or homeostasis?
Provedor de dados: 42
Autores: Quezada,Landys A. Lopez; McKerrow,James H.
Serpins are a structurally conserved family of macromolecular inhibitors found in numerous biological systems. The completion and annotation of the genomes of Schistosoma mansoni and Schistosoma japonicum has enabled the identification by phylogenetic analysis of two major serpin clades. S. mansoni shows a greater multiplicity of serpin genes, perhaps reflecting adaptation to infection of a human host. Putative targets of schistosome serpins can be predicted from the sequence of the reactive center loop (RCL). Schistosome serpins may play important roles in both post-translational regulation of schistosome-derived proteases, as well as parasite defense mechanisms against the action of host proteases.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schistosomiasis; Inhibitor; Protease; Serpin; Genome.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000200025

Detection of proteases from Sporosarcina aquimarina and Algoriphagus antarcticus isolated from Antarctic soil
Provedor de dados: 42
Autores: Santos,Anderson F.; Pires,Fabiano; Jesus,Hugo E.; Santos,André L.S.; Peixoto,Raquel; Rosado,Alexandre S.; D'Avila-Levy,Claudia M.; Branquinha,Marta H.
Two psychrophilic bacterial samples were isolated from King George Island soil, in Antarctica. The phylogenetic analysis based on the 16S rRNA (rrs) gene led to the correlation with the closest related isolates as Sporosarcina aquimarina (99%) and Algoriphagus antarcticus(99%), with query coverage of 99% and 98%, respectively.The spent culture media from both isolates displayed proteolytic activities detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis containing gelatin as protein substrate. Under the employed conditions, S. aquimarina showed a 55 kDa protease with the best activity detected at pH 7.0 and at 27°C. A. antarcticusalso showed a single extracellular protease, however its molecular mass was around 90kDa and its best activity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Algoriphagus antarcticus; Antarctica; Sporosarcina aquimarina; Protease.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100109

Efeito da protease sobre o coeficiente de metabolizabilidade dos nutrientes em frangos de corte
Provedor de dados: 45
Autores: Matias,C.F.Q.; Rocha,J.S.R.; Pompeu,M.A.; Baião,R.C.; Baião,N.C.; Lara,L.J.C.; Clímaco,W.L.S.; Pereira,L.F.P.; Caldas,E.O.; Teixeira,M.P.F.; Cardeal,P.C.
Avaliaram-se os efeitos da protease sobre o coeficiente de metabolizabilidade dos nutrientes em dietas contendo farinha de penas (2,0% na fase inicial e 3,0% na fase de crescimento) para frangos de corte machos, Cobb(r), de um a 32 dias de idade. Foram alojadas 336 aves em gaiolas metálicas equipadas com bandeja para coleta total de excretas. Foi utilizado delineamento inteiramente ao acaso, em arranjo fatorial 2x2 (com e sem adição da enzima protease 0,05% e duas valorizações da matriz nutricional da enzima), sendo sete repetições por tratamento, de 12 aves cada. Observou-se, na fase inicial, maior CMPB (P≤0,05) para as aves que, independentemente da adição de protease, consumiram ração com valorização da matriz nutricional da enzima. Não houve efeito dos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coeficiente de metabolizabilidade; Enzimas exógenas; Frango de corte; Protease.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200492

Complexos enzimáticos sobre a energia metabolizável e digestibilidade dos nutrientes do milheto para frangos de corte
Provedor de dados: 45
Autores: Moura,F.A.S.; Dourado,L.R.B.; Farias,L.A.; Lopes,J.B.; Lima,S.B.P.; Fernandes,M.L.
RESUMO Objetivou-se avaliar o efeito de complexos enzimáticos sobre a energia metabolizável e o coeficiente de digestibilidade de nutrientes do milheto para frangos de corte. Quinhentos e setenta e seis frangos machos foram distribuídos em 36 gaiolas, com três tratamentos: T1 - composição de milheto sem complexo enzimático; T2 - composição de milheto com complexo enzimático (CES) e T3 - composição de milheto com complexo enzimático (CEV). Os tratamentos foram definidos com base em seis dietas (três dietas referências e três dietas testes). As dietas testes foram obtidas pela substituição de 40% da dieta referência por milheto inteiro, e a adição de enzimas consistiu de dois complexos enzimáticos: CES, constituído pelas enzimas fitase, protease, xilanase,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alimento alternativo; Dietas; Enzimas; Protease; Xilanase.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300990

Kinetics Study of Extracellular Detergent Stable Alkaline Protease from Rhizopus oryzae
Provedor de dados: 52
Autores: Mushtaq,Zareena; Irfan,Muhammad; Nadeem,Muhammad; Naz,Mammona; Syed,Quratulain.
In this study, extracellular alkaline protease was produced from Rhizopus oryzae in submerged fermentation using dairy waste (whey) as a substrate. Fermentation kinetics was studied and various parameters were optimized. The strain produced maximum protease at initial medium pH of 6.0 medium depth of 26 mm, inoculum size of 2% at incubation temperature of 35ºC for 168 h of fermentation. Alkaline protease was purified to homogeneity by ammonium sulphate fractionation followed by sephadex G-100 chromatography. The molecular mass of alkaline protease was 69 kDa determined by 10% SDS-PAGE. The optimum pH and temperature of alkaline protease was 9.0 and 40ºC, respectively. Metal profile of the enzyme showed that the enzyme was non-metallic in nature. The Km ,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kinetics; Purification; Characterization; Protease; Detergent stability; Rhizopus oryzae.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200175

Characterization of Nomuraea rileyi strains using polymorphic DNA, virulence and enzyme activity
Provedor de dados: 52
Autores: Vargas,Lúcia Rosane Bertholdo; Rossato,Marcelo; Ribeiro,Rute Terezinha da Silva; Barros,Neiva Monteiro de.
The characterization of entomopathogenic microorganisms is important for the selection of more effective strains for use in integrated pest-control programs. Five Nomuraea rileyi strains (SA86101, GU87401, SR86151, CG128 and VA9101) were characterized using random amplified polymorphic DNA (RAPD) analysis, virulence studies and assessment of chitinolytic and proteolytic activity. RAPD analysis divided the strains into two groups with a similarity coefficient of 0,76%, group 1 consisting of strains SA86101, GU87401 and SR86151 and group 2 of strains CG128 and VA9101. The LT50 varied from 165h with strain VA9101 to 246h with strain GU87401. Chitinolytic and proteolytic activity of the fungi after 144h growth in minimal medium were tested using colloidal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nomuraea rileyi; Anticarsia gemmatalis; Chitinase; Protease; RAPD; Virulence.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100003

Nematicidal activity of Paecilomyces marquandii proteases on infective larvae of Ancylostoma spp
Provedor de dados: 52
Autores: Genier,Hugo Leonardo André; Queiroz,José Humberto de; Braga,Fabio Ribeiro; Soares,Filippe Elias de Freitas; Araújo,Jackson Victor de; Pinheiro,Iara Rebouças.
ABSTRACT The present study aimed to evaluate the action of Paecilomyces marquandii proteases on Ancylostoma spp L3. White halos in the zymogram confirmed the proteolytic action. Difference (p &lt;0.01) between the number of L3 in the differents groups was found, with 41.4% of reduction of Ancylostoma spp. L3 before 24 hours.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Nematophagous fungi; Protease; Zoonosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100423

Immobilization of the Magnetic Nanoparticles with Alkaline Protease Enzyme Produced by Enterococcus hirae and Pseudomonas aeruginosa Isolated from Dairy Effluents
Provedor de dados: 52
Autores: Masi,Chandran; Chandramohan,C.; Ahmed,M.Fazil.
ABSTRACT Protease is an enzyme which has a wide range of applications in various fields. Extracellular protease was produced from Pseudomonas aeruginosa and Enterococcus hirae which were isolated from the effluents of diary industries. Protease immobilized with super paramagnetic nanoparticles was characterized by DLS, XRD and TEM methods in relation to their size and structure. The protease enzyme was bound to magnetic nanoparticles via surface transformation technique including Silica coated magnetic Nano composite, amine and cysteine functioned Nano composite formation. Successful binding of protease onto the particles was confirmed by TEM imaging. The maximal enzyme activity of immobilized protease was determined using universal protease assay and was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protease; Super paramagnetic Nanoparticle; Nano composite; Immobilization; Activity and Stability.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100462

Peptidase with Keratinolytic Activity Secreted by Aspergillus terreus During Solid-State Fermentation
Provedor de dados: 52
Autores: Siqueira,Ana Claudia Rodrigues de; Rosa,Nathalia Gonsales da; Motta,Cristina Maria Souza; Cabral,Hamilton.
The aim of this study was to evaluate peptidase production by Aspergillus terreus in solid-state bioprocess and evaluate its parameters. The best conditions were 5.0 g of wheat bran as substrate, incubation temperature 30°C, inoculum 2.0x105spores/g and 75% saline volume, with production reaching 677 U/mL (5400 U/g culture medium) after 72 h of fermentation. Biochemical characterization of the crude enzymatic extract showed the optimum pH and temperature of 6.5 and 55°C, respectively. The stability at different temperatures and pH values showed that the extract could endure different pH. The evaluation of the ions influence and inhibitors proved that the enzyme required an ion for better activity, which was corroborated with the inhibition of EDTA and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protease; Solid-state fermentation; Keratinase; Wheat bran; Aspergillus terreus.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400514

Prevalence of resistance-associated mutations in Human Immunodeficiency Virus type 1-positive individuals failing HAART in Rio de Janeiro, Brazil
Provedor de dados: 55
Autores: Varella,Rafael Brandão; Ferreira,Selma Baía; Castro,Márcia Braga de; Tavares,Marisa Dias; Zalis,Mariano Gustavo.
We investigated the occurrence of HIV-1 antiretroviral resistance in individuals failing to respond to highly active antiretroviral therapy (HAART) attended by RENAGENO from 2001-2004. One hundred and seventeen patients were selected for this study; their plasma viral RNA was extracted and the PR and RT genes sequenced to examine subtype, genetic polymorphisms and mutations associated with resistance to antiretroviral drugs. HIV-1 sequence analysis showed that 86/100 (86%) were infected with subtype B, 7/100 (7%) with subtype F and 7/100 (7%) with RT/PR hybrid forms (2 D/B, 2 F/B, 2 B/F and 1 D/F). In 14 (12%) of the samples, the subtype was not determined. The prevalence of resistance mutations was high (93.1%), mainly in the RT gene. The most prevalent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV-1; HAART; Reverse transcriptase; Protease; Resistance.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500007

Functions of the extracellular matrix and matrix degrading proteases during tumor progression
Provedor de dados: 56
Autores: Liaw,L.; Crawford,H.C.
Cell interactions with extracellular matrices are important to pathological changes that occur during cell transformation and tumorigenesis. Several extracellular matrix proteins including fibronectin, thrombospondin-1, laminin, SPARC, and osteopontin have been suggested to modulate tumor phenotype by affecting cell migration, survival, or angiogenesis. Likewise, proteases including the matrix metalloproteinases (MMPs) are understood to not only facilitate migration of cells by degradation of matrices, but also to affect tumor formation and growth. We have recently demonstrated an in vivo role for the RGD-containing protein, osteopontin, during tumor progression, and found evidence for distinct functions in the host versus the tumor cells. Because of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Matrix metalloproteinase; Osteopontin; Carcinoma; Protease.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700002

Antifungal and proteolytic activities of endophytic fungi isolated from Piper hispidum Sw
Provedor de dados: 58
Autores: Orlandelli,Ravely Casarotti; Almeida,Tiago Tognolli de; Alberto,Raiani Nascimento; Polonio,Julio Cesar; Azevedo,João Lúcio; Pamphile,João Alencar.
Endophytes are being considered for use in biological control, and the enzymes they secrete might facilitate their initial colonization of internal plant tissues and direct interactions with microbial pathogens. Microbial proteases are also biotechnologically important products employed in bioremediation processes, cosmetics, and the pharmaceutical, photographic and food industries. In the present study, we evaluated antagonism and competitive interactions between 98 fungal endophytes and Alternaria alternata, Colletotrichum sp., Phyllosticta citricarpa and Moniliophthora perniciosa. We also examined the proteolytic activities of endophytes grown in liquid medium and conducted cup plate assays. The results showed that certain strains in the assemblage of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antagonism; Competitive interaction; Dual culture; Cup plate; Protease.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200359

Culturable fungal diversity of shrimp Litopenaeus vannamei boone from breeding farms in Brazil
Provedor de dados: 58
Autores: Silva,Lidiane Roberta Cruz da; Souza,Odacy Camilo de; Fernandes,Maria José dos Santos; Lima,Débora Maria Massa; Coelho,Rosalie Reed Rodrigues; Souza-Motta,Cristina Maria.
Litopenaeus vannamei, which is the most common shrimp species cultivated in the northeast of Brazil, is very susceptible to microbial diseases, and this consequently affects productivity. There are reports of bacteria, viruses and protozoa in these shrimp, but not fungi. This study aims to isolate and identify fungi present in shrimp Litopenaeus vannamei, and in their nursery waters, at two breeding farms in Brazil. The pathogenic potential of the isolates was assessed through the qualitative detection of proteases and aflatoxin B production. The 146 isolated fungi comprised 46 species. Aspergillus, Penicillium and Furarium were the three most relevant genera and Aspergillus flavus was the predominant species with a total of 33 isolates. Most of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shrimp; Fungi; Water; Protease; Aflatoxin B.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100007

Detection of trypsin inhibitor in seeds of Eucalyptus urophylla and its influence on the in vitro growth of the fungi Pisolithus tinctorius and Rhizoctonia solani
Provedor de dados: 58
Autores: Tremacoldi,Célia Regina; Pascholati,Sérgio Florentino.
Inhibitors of plant proteases can regulate the hydrolysis of proteins inside the cells and also participate in the mechanisms of plant defense against herbivore insects and pathogens. Here, we demonstrated that seeds of Eucalyptus urophylla exhibit activities of trypsin and papain inhibitors, two proteases commonly found in living cells. Low amounts of proteins of the crude protein extract of seeds and fractions partially purified by gel filtration, with inhibitory activity against trypsin, inhibited in vitro the mycelial growth of a compatible isolate of the ectomycorrhizal fungus Pisolithus tinctorius and allowed an unsatisfactory growth of another isolate from Pinus taeda, considered incompatible for this eucalyptus species. The same amounts of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protease; Inhibitor; Trypsin; Pisolithus tinctorius; Rhizoctonia solani.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400001

Concomitant production of protease and lipase by Bacillus licheniformis VSG1: production, purification and characterization
Provedor de dados: 58
Autores: Sangeetha,R.; Geetha,A.; Arulpandi,I.
This study was aimed at producing protease and lipase simultaneously on a common medium by Bacillus licheniformis VSG1, which was isolated from a tannery effluent. The effect of media composition with respect to protein source, lipid source and emulsifier on the production of protease and lipase was analysed. Both those enzymes were produced under optimized conditions like pH, temperature and incubation time. The enzyme mixture comprising of both protease and lipase was purified by ammonium sulphate precipitation, dialysis and gel filtration chromatography to obtain 20-fold pure enzymes. The purified enzyme mixture was characterized to determine the optimum pH and temperature of protease and lipase, the response of the enzymes to inhibitors, additives and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus licheniformis; Protease; Lipase; Concomitant production; Purification.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100026

Screening of Mucor spp. for the production of amylase, lipase, polygalacturonase and protease
Provedor de dados: 58
Autores: Alves,Maria Helena; Campos-Takaki,Galba M.; Porto,Ana Lúcia Figueiredo; Milanez,Adauto Ivo.
Fungi are well known by their ability to excrete enzymes into the environment. Among them, representatives of Mucor Fresen. have important biotechnological potential and some of them produce industrial enzymes. This work studied amylase, lipase, polygalacturonase and protease production by fifty-six isolates of Mucor belonging to 11 different taxa, selected from herbivores dung using solid media. The results showed that the majority of the isolates presented several enzymatic activities with predominance of polygalacturonase (96%), followed by amylase (84%), protease (82%) and lipase (66%).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fungal enzymes; Mucor; Amylase; Lipase; Polygalacturonase; Protease; Herbivorous dung.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400009

Effect of the culture conditions on the production of an extracellular protease by thermophilic Bacillus sp and some properties of the enzymatic activity
Provedor de dados: 58
Autores: Silva,Camila Rocha da; Delatorre,Andréia Boechat; Martins,Meire Lelis Leal.
Protease production by thermophilic Bacillus sp strain SMIA-2 cultivated in liquid cultures containing 1% maltose as a carbon source and supplemented with whey protein (0.1%) and corn steep liquor (0.3%) reached a maximum at 14 h, with levels of 42 U/mg protein. The microorganism was capable of utilizing a wide range of carbon sources, but protease activity varied according the carbon source. Starch and maltose were the best carbon sources in the present study for protease secretion, while lactose and sucrose were less effective. Increasing maltose concentration in the medium until 1%, improved the growth of the organism and the enzyme activity. Regarding the amounts of corn steep liquor and whey protein in the medium, the concentrations of 0.2% and 0.1%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protease; Thermophilic bacterium; Bacillus sp.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200012

Enzymatic comparison and mortality of Beauveria bassiana against cabbage caterpillar Pieris brassicae LINN
Provedor de dados: 58
Autores: Dhawan,Manish; Joshi,Neelam.
Abstract Beauveria bassiana, an entomopathogenic fungus, is the alternative biocontrol agent exploited against major economic crop pests. Pieris brassicae L. is an emerging pest of the Brassicaceae family. Therefore, in the present study, fungal isolates of Beauveria bassiana, viz. MTCC 2028, MTCC 4495, MTCC 6291, and NBAII-11, were evaluated for their virulence against third instar larvae of P. brassicae. Among all these fungal isolates, maximum mortality (86.66%) was recorded in B. bassiana MTCC 4495 at higher concentration of spores (109 conidia/ml), and the minimum mortality (30.00%) was recorded in B. bassiana MTCC 6291 at a lower concentration (107 conidia/ml) after ten days of treatment. The extracellular cuticle-degrading enzyme activities of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chitinase; Entomopathogenic fungi; Lipase; Mortality; Protease.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300522

Studies on the stability of protease from Bacillus sp. and its compatibility with commercial detergent
Provedor de dados: 58
Autores: Nascimento,Wellingta Cristina Almeida do; Martins,Meire Lelis Leal.
Enzymes, and particularly proteases, have become an important and indispensable part of industrial processes such as laundry detergents, pharmaceuticals and food products. Detergents such as Tide®, Ariel® and Biz® contain proteolytic enzymes, most of them produced by members of the genus Bacillus. This paper describes the compatibility of protease produced by the thermophilic Bacillus sp, with commercial laundry detergent. Stability studies indicated that this enzyme retained about 95% and 74% of its maximum activity after 1h at 60ºC in the presence of glycine in combination with MnSO4 and CaCl2, respectively. No inhibitory effect was observed at 1.0-5.0 mM of EDTA. Triton X-100 inhibited the enzyme in all the concentrations tested. The enzyme was unstable...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protease; Thermophilic bacterium; Bacillus sp.; Detergents.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300020

Halotolerant bacteria in the São Paulo Zoo composting process and their hydrolases and bioproducts
Provedor de dados: 58
Autores: Oliveira,Lilian C.G.; Ramos,Patricia Locosque; Marem,Alyne; Kondo,Marcia Y.; Rocha,Rafael C.S.; Bertolini,Thiago; Silveira,Marghuel A.V.; Cruz,João Batista da; Vasconcellos,Suzan Pantaroto de; Juliano,Luiz; Okamoto,Debora N.
Halophilic microorganisms are able to grow in the presence of salt and are also excellent source of enzymes and biotechnological products, such as exopolysaccharides (EPSs) and polyhydroxyalkanoates (PHAs). Salt-tolerant bacteria were screened in the Organic Composting Production Unit (OCPU) of São Paulo Zoological Park Foundation, which processes 4 ton/day of organic residues including plant matter from the Atlantic Rain Forest, animal manure and carcasses and mud from water treatment. Among the screened microorganisms, eight halotolerant bacteria grew at NaCl concentrations up to 4 M. These cultures were classified based on phylogenetic characteristics and comparative partial 16S rRNA gene sequence analysis as belonging to the genera Staphylococcus,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Halophilic; Protease; Lipase; Amylase; Cellulase.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200347

Hydrolytic potential of a psychrotrophic Pseudomonas isolated from refrigerated raw milk
Provedor de dados: 58
Autores: Corrêa,Ana Paula F.; Daroit,Daniel J.; Velho,Renata V.; Brandelli,Adriano.
The production of extracellular hydrolases by a psychrotrophic bacterium isolated from refrigerated raw milk, and identified as a Pseudomonas sp. belonging to the Pseudomonas jenssenii group, was studied. This bacterium produced proteolytic and lipolytic enzymes in all media investigated (skim milk, cheese whey, casein broth, and tryptone soy broth). High levels of α-glucosidase were produced in skim milk broth. Hydrolytic enzymes detected in skim milk broth are of particular concern, indicating that these enzymes could be produced by Pseudomonas sp. during the cold storage of raw milk, contributing to the spoilage problem in milk and dairy products.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas; Hydrolases; Protease; Lipase; Glycosidase.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400034

Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach
Provedor de dados: 58
Autores: Shabbiri,Khadija; Adnan,Ahmad; Jamil,Sania; Ahmad,Waqar; Noor,Bushra; Rafique,H.M.
Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158 grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the Plackett-Burman design and four significant variables (soybean meal, wheat bran, (NH4)2SO4 and inoculum size were further optimized via central composite design (CCD) using a response surface methodological approach. Using the optimal factors (soybean meal 12.0g, wheat bran 8.50g, (NH4)2SO4) 0.45g and inoculum size 3.50%), the rate of protease production was found to be twofold higher in the optimized medium as compared to the unoptimized reference medium.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brevibacterium linens; Protease; Solid-state fermentation; Plackett-Burman Design; Central Composite Design (CCD).
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300031

Production of hydrolytic enzymes by the plant pathogenic fungus Myrothecium verrucaria in submerged cultures
Provedor de dados: 58
Autores: Moreira,Fabiana Guillen; Reis,Simone dos; Costa,Maria Aparecida Ferreira; Souza,Cristina Giatti Marques de; Peralta,Rosane Marina.
The capability of the plant pathogenic fungus Myrothecium verrucaria to produce extracellular hydrolytic enzymes in submerged cultures was studied using several substrates. The fungus was able to produce different depolymerases and glycosidases, being xylanase, pectinase and protease the most important. Lipase was found in cultures developed in the presence of olive oil, while protease activity was detected in all cultures. Xylanase and pectinase were optimally active at pH 4.5-5.5, while protease was active in a large range of pH 3.5 to 11.0. All three enzymes were maximally active at 40ºC and they were stable for several hours at temperature up to 50ºC.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carbohydrases; Hydrolytic enzymes; Myrothecium verrucaria; Protease.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100002

Production and properties of an extracellular protease from thermophilic Bacillus sp
Provedor de dados: 58
Autores: Nascimento,Wellingta Cristina Almeida do; Martins,Meire Lelis Leal.
Protease production by thermophilic Bacillus sp strain SMIA-2 cultivated in liquid cultures containing trisodium citrate reached a maximum in 9h, with levels of 1.93U/mg protein. The microorganism utilized several carbon sources for the production of protease. Starch was the best substrate, followed by trisodium citrate, citric acid and sucrose. Among the various organic and inorganic nitrogen sources, ammonium nitrate was found to be the best. Studies on the protease characterization revealed that the optimum temperature of this enzyme was 60ºC. The enzyme was stable for 2h at 30ºC, while at 40ºC and 80ºC, 14% and 84% of the original activities were lost, respectively. The optimum pH of the enzyme was found to be 8.0. After incubation of crude enzyme...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protease; Thermophilic bacterium; Bacillus sp.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100015

Optimization and characterization of alkaline protease and carboxymethyl-cellulase produced by Bacillus pumillus grown on Ficus nitida wastes
Provedor de dados: 58
Autores: Gomaa,Eman Zakaria.
The potentiality of 23 bacterial isolates to produce alkaline protease and carboxymethyl-cellulase (CMCase) on Ficus nitida wastes was investigated. Bacillus pumillus ATCC7061 was selected as the most potent bacterial strain for the production of both enzymes. It was found that the optimum production of protease and CMCase were recorded at 30 °C, 5% Ficus nitida leaves and incubation period of 72 h. The best nitrogen sources for protease and CMCase production were yeast extract and casein, respectively. Also maximum protease and CMCase production were reported at pH 9 and pH 10, respectively. The enzymes possessed a good stability over a pH range of 8-10, expressed their maximum activities at pH10 and temperature range of 30-50 °C, expressed their maximum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alkaliphillic; Protease; Carboxymethylcellulase; Production; Characterization.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200033

Immunoglobulin G proteolytic activity of Actinobacillus actinomycetemcomitans
Provedor de dados: 58
Autores: Nakanishi,Fernanda Akemi; Avila-Campos,Mario Julio; Kamiji,Nádia Hizuru; Itano,Eiko Nakagawa.
Actinobacillus actinomycetemcomitans produces a protease to human immunoglobulin G that is an important evasion mechanism. In this study, the proteolytic activity of A. actinomycetemcomitans strain ATCC 43718 on human immunoglobulin G associated with culture supernatant concentrations, the growth period and the period of incubation with immunoglobulin G were evaluated by an enzyme linked immunosorbent assay. The protease fraction was detected by Sephadex G 150 chromatography. The results showed that A. actinomycetemcomitans produced a protease to human immunoglobulin G in the culture supernatant, and the highest activity was achieved witen the concentration was 27.5 mug protein/mL, after culturing for 72 hours and incubating with IgG for 24 hours. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunoglobulin G; Actinobacillus actinomycetemcomitans; ELISA; Protease.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100008

Enzimas exógenas em dietas de frangos de corte: desempenho
Provedor de dados: 65
Autores: Barbosa,Nei André Arruda; Sakomura,Nilva Kazue; Bonato,Melina Aparecida; Hauschild,Luciano; Oviedo-Rondon,Edgar.
O experimento foi conduzido para avaliar a eficiência de enzimas exógenas em dietas sobre o desempenho de frangos de corte. Um total de 1440 pintos de corte machos (Cobb 500®) foram distribuídos em um delineamento inteiramente casualizado em esquema fatorial 2x2 (duas dietas com diferentes densidades nutricionais; com e sem a adição enzimática) com 8 repetições de 45 aves em cada unidade experimental. As dietas com diferentes densidades nutricionais foram: controle positivo (níveis normais) e um controle negativo (com redução de 4,3 e 4,5% de energia metabolizável; 16,7 e 17,7% de cálcio e 35 e 42,7% de fósforo, nas fases inicial e de crescimento, respectivamente). A suplementação enzimática consistiu da combinação das enzimas fitase (100g t-1) e amilase,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aves; Fitase; Amilase; Protease; Xilanase.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000800027