Consulta: subjectFacets:"Quantitative real-time PCR"
Registros recuperados: 8
Data/hora: 10/06/2024 18:34:03

Cloning and expression analysis of the chitinase gene Ifu-chit2 from Isaria fumosorosea
Provedor de dados: Genetics and Molecular Biology
Autores: Meng,Huimin; Wang,Zhangxun; Meng,Xiangyun; Xie,Ling; Huang,Bo.
Entomopathogenic fungi can produce a series of chitinases, some of which function synergistically with proteases and other hydrolytic enzymes to degrade the insect cuticle. In the present study, the chitinase gene Ifu-chit2 from Isaria fumosorosea was investigated. The Ifu-chit2 gene is 1,435-bp long, interrupted by three short introns, and encodes a predicted protein of 423 amino acids with a 22 residue signal peptide. The predicted Ifu-Chit2 protein is highly homologous to Beauveria bassiana chitinase Bbchit2 and belongs to the glycohydrolase family 18. Ifu-Chit2 was expressed in Escherichia coli to verify chitinase activity, and the recombinant enzyme exhibited activity with a colloidal chitin substrate. Furthermore, the expression profiles of Ifu-chit2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isaria fumosorosea; Chitinase; Prokaryotic expression; Quantitative real-time PCR.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300381

Proteomic analysis of a clavata-like phenotype mutant in Brassica napus
Provedor de dados: Genetics and Molecular Biology
Autores: Zhu,Keming; Zhang,Weiwei; Sarwa,Rehman; Xu,Shuo; Li,Kaixia; Yang,Yanhua; Li,Yulong; Wang,Zheng; Cao,Jun; Li,Yaoming; Tan,Xiaoli.
Abstract Rapeseed is one of important oil crops in China. Better understanding of the regulation network of main agronomic traits of rapeseed could improve the yielding of rapeseed. In this study, we obtained an influrescence mutant that showed a fusion phenotype, similar with the Arabidopsis clavata-like phenotype, so we named the mutant as Bnclavata-like (Bnclv-like). Phenotype analysis illustrated that abnormal development of the inflorescence meristem (IM) led to the fused-inflorescence phenotype. At the stage of protein abundance, major regulators in metabolic processes, ROS metabolism, and cytoskeleton formation were seen to be altered in this mutant. These results not only revealed the relationship between biological processes and inflorescence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brassica napus; Proteomic; Inflorescence meristem (IM); Bnclavata-like (Bnclv-like); Quantitative real-time PCR.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100308

Comparative proteomic analysis of indica and japonica rice varieties
Provedor de dados: Genetics and Molecular Biology
Autores: Yang,Yanhua; Zhu,Keming; Xia,Hengchuan; Chen,Liang; Chen,Keping.
Indica and japonica are two main subspecies of Asian cultivated rice (Oryza sativa L.) that differ clearly in morphological and agronomic traits, in physiological and biochemical characteristics and in their genomic structure. However, the proteins and genes responsible for these differences remain poorly characterized. In this study, proteomic tools, including two-dimensional electrophoresis and mass spectrometry, were used to globally identify proteins that differed between two sequenced rice varieties (93-11 and Nipponbare). In all, 47 proteins that differed significantly between 93-11 and Nipponbare were identified using mass spectrometry and database searches. Interestingly, seven proteins were expressed only in Nipponbare and one protein was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Indica and japonica rice; Molecular marker; Proteomics; Quantitative real-time PCR; Unique proteins.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500008

Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements
Provedor de dados: Genetics and Molecular Biology
Autores: Liang,Qiaoyi; Ding,Jiayi; Zheng,Shu.
Up-regulation of human endogenous retroviruses (HERVs) is associated with many diseases, including cancer. In this study, an H family HERV (HERV-H)-related gene was identified and characterized. Its spliced transcript lacks protein-coding capacity and may belong to the emerging class of noncoding RNAs (ncRNAs). The 1.3-kb RNA consisting of four exons is transcribed from an Alu element upstream of a 5.0-kb structurally incomplete HERV-H element. RT-PCR and quantitative RT-PCR results indicated that expression of this HERV-related transcript was negatively associated with colon, stomach, and kidney cancers. Its expression was induced upon treatment with DNA methylation and histone deacetylation inhibitors. A BLAT search using long terminal repeats (LTRs)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endogenous retrovirus; Noncoding RNA; RT-PCR; Quantitative real-time PCR; Transcription.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400005

Effect of oxygen and temperature on the dynamic of the dominant bacterial populations of pig manure and on the persistence of pig-associated genetic markers, assessed in river water microcosms
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Marti, R.; Mieszkin, Sophie; Solecki, O.; Pourcher, A. -m.; Hervio-heath, Dominique; Gourmelon, Michele.
Aims: The aim is to evaluate the dynamic of Bacteroides-Prevotella and Bacillus-Streptococcus-Lactobacillus populations originating from pig manure and the persistence of pig-associated markers belonging to these groups according to temperature and oxygen. Methods and Results: River water was inoculated with pig manure and incubated under microaerophilic and aerobic conditions, at 4 and 20 degrees C over 43 days. The diversity of bacterial populations was analysed by capillary electrophoresis-single-strand conformation polymorphism. The persistence of the pig-associated markers was measured by real-time PCR and compared with the survival of Escherichia coli and enterococci. Decay was characterized by the estimation of the time needed to produce a 1-log...
Tipo: Text
Palavras-chave: Lactobacillus amylovorus; Microbial source tracking; Oxygen; Persistence; Pig-associated Bacteroidales markers; Quantitative real-time PCR; River water; Temperature.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00053/16470/14534.pdf

Diagnostic moléculaire de l'origine des contaminations fécales dans l'environnement littoral - Développement de marqueurs Bacteroidales spécifiques de l'hôte
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Mieszkin, Sophie.
Human and animal faecal pollution affects environmental water in inland and coastal areas, with negative implications for recreational uses, public safety and shellfish sanitary status due to the presence of enteric pathogens. Starting from 2011, the revised Bathing Water European Directive (2006/7/CE) requires the establishment of bathing water profiles with an inventory and study of the pollution sources likely to affect water quality. The faecal microbiological indicators used in these regulations, Escherichia coli and enteroccoci, cannot distinguish between human and animal faecal contamination. Thus, alternative methods, that focus on target microorganisms such as bacteria belonging to the Bacteroidales order were developed or are being developed to...
Tipo: Text
Palavras-chave: Contaminations fécales humaines; Porcines et ruminants; Bacteroidales; Gènes codant les ARNr 16S; PCR quantitative en temps réel; Eaux; Persistance; Huîtres; Human; Porcine and ruminant faecal contaminations; Bacteroidales; 16S rRNA genes; Quantitative real-time PCR; Water; Persistence; Oyster.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00021/13206/10258.pdf

PHLOEM PROMOTERS IN TRANSGENIC SWEET ORANGE ARE DIFFERENTIALLY TRIGGERED BY Candidatus Liberibacter asiaticus
Provedor de dados: Revista Brasileira de Fruticultura
Autores: MIYATA,LUZIA YURIKO; HARAKAVA,RICARDO; ATTÍLIO,LÍSIA BORGES; MENDES,BEATRIZ MADALENA JANUZZI; LOPES,JOÃO ROBERTO SPOTTI; COLETTA-FILHO,HELVÉCIO DELLA; SOUZA,ALESSANDRA ALVES DE; MOURÃO FILHO,FRANCISCO DE ASSIS ALVES.
ABSTRACT  The use of promoters preferentially expressed in specific plant tissues is a desirable strategy to search for resistance for pathogens that colonize these tissues. The bacterium Candidatus Liberibacter asiaticus (Las), associated with huanglongbing disease (HLB) of citrus, colonizes phloem vessels. Some promoters, besides conferring tissue-specific expression, can also respond to the presence of the pathogen. The objective of the present study was to verify if the presence of Las could modulate the activation of the phloem-specific promoters AtPP2 (Arabidopsis thaliana phloem protein 2), AtSUC2 (A. thaliana sucrose transporter 2) and CsPP2 ( pCitrus phloemrotein 2), known to be expressed in Citrus sinensis phloem. ‘Hamlin’ sweet orange plants...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus sinensis; Diaphorina citri; Genetic transformation; GUS; Huanglongbing; Quantitative real-time PCR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000300101

Differential expression of genes in follicular cells of swines
Provedor de dados: Revista Brasileira de Zootecnia
Autores: Miranda-Furtado,Cristiana Libardi; Silva,Priscila Vendramini; Guimarães,Marta Fonseca Martins; Serão,Nicola Vergara Lopes; Guimarães,José Domingos; Guimarães,Simone Eliza Facioni.
The main purpose of the present study was to identify for candidate genes related to ovulation in swines. To do so, it was investigated in ovarian follicular cells through quantitative real-time PCR the differential expression of the following genes: steroidogenic acute regulator (STAR), GATA-binding protein 4 (GATA), prostaglandin F2α (PGF2α), progesterone receptor (P4R), follicle-stimulating hormone receptor (FSHR), and cytochrome P450 aromatase (CYP19). These genes encode hormone receptors (FSHR and P4R), hormone (PGF2α), steroidogenic proteins (STAR and CYP19) and transcription factor (GATA). Folicular cells were collected from sows with high and low number of piglets/litters during the follicular phase of the estrus cycle. There was difference in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candidate gene; Litter size; Ovulation rate; Pig production; Quantitative real-time PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000500012