Consulta: subjectFacets:"RAW 264.7 cells"
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Data/hora: 08/06/2024 15:26:29

Effects of propofol on lipopolysaccharide-induced expression and release of HMGB1 in macrophages
Provedor de dados: 56
Autores: Wang,T.; Wei,X.Y.; Liu,B.; Wang,L.J.; Jiang,L.H.
This study aimed to determine the effects of different concentrations of propofol (2,6-diisopropylphenol) on lipopolysaccharide (LPS)-induced expression and release of high-mobility group box 1 protein (HMGB1) in mouse macrophages. Mouse macrophage cell line RAW264.7 cells were randomly divided into 5 treatment groups. Expression levels of HMGB1 mRNA were detected using RT-PCR, and cell culture supernatant HMGB1 protein levels were detected using enzyme-linked immunosorbent assay (ELISA). Translocation of HMGB1 from the nucleus to the cytoplasm in macrophages was observed by Western blotting and activity of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in the nucleus was detected using ELISA. HMGB1 mRNA expression levels increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propofol; HMGB1; LPS; RAW 264.7 cells; NF-κB.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400286