Consulta: subjectFacets:"Renin"
Registros recuperados: 6
Data/hora: 17/06/2024 15:48:28

Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells
Provedor de dados: 56
Autores: Andrade,A.Q.; Casarini,D.E.; Schor,N.; Boim,M.A.
Renin is an enzyme involved in the stepwise generation of angiotensin II. Juxtaglomerular cells are the main source of plasma renin, but renin activity has been detected in other cell types. In the present study we evaluated the presence of renin mRNA in adult male Wistar rat and mouse (C-57 Black/6) mesangial cells (MC) and their ability to process, store and release both the active and inactive forms of the enzyme. Active renin and total renin content obtained after trypsin treatment were estimated by angiotensinogen consumption analyzed by SDS-PAGE electrophoresis and quantified by angiotensin I generation by HPLC. Renin mRNA, detected by RT-PCR, was present in both rat and mouse MC under basal conditions. Active renin was significantly higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renin; Prorenin; Mesangial cell; Kidney.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100003

Bi-directional actions of estrogen on the renin-angiotensin system
Provedor de dados: 56
Autores: Brosnihan,K.B.; Senanayake,P.S.; Li,P.; Ferrario,C.M.
Estrogen stimulates the renin-angiotensin system by augmenting both tissue and circulating levels of angiotensinogen and renin. We show, however, that angiotensin converting enzyme (ACE) activity in the circulation and in tissues is reduced in two animal models of postmenopausal chronic hormone replacement. We observed a reduction of ACE activity in association with a significant increase in plasma angiotensin I (Ang I) and hyperreninemia in ovariectomized monkeys treated with Premarin (conjugated equine estrogen) replacement for 30 months. Plasma angiotensin II (Ang II) levels were not increased in monkeys treated with estrogen, suggesting that the decrease in ACE curtailed the formation of the peptide. The Ang II/Ang I ratio, an in vivo index of ACE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renin; Angiotensin converting enzyme; Angiotensin peptides; Angiotensin receptors; Bradykinin; Nitric oxide; Kinins; Vasodilation; Hormone replacement.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400001

Hemodynamic and hormonal actions of adrenomedullin
Provedor de dados: 56
Autores: Nicholls,M.G.
Adrenomedullin, a 52-amino acid residue peptide, has numerous biological actions which are of potential importance to cardiovascular homeostasis, growth and development of cardiovascular tissues and bone, prevention of infection, and regulation of body fluid and electrolyte balance. Studies in man using intravenous infusion of the peptide have demonstrated that, at plasma levels detected after myocardial infarction or in heart failure, adrenomedullin reduces arterial pressure, increases heart rate and cardiac output, and activates the sympathetic and renin-angiotensin systems but suppresses aldosterone. The thresholds for these responses differ, being lower under some experimental circumstances for arterial pressure than for the other biological effects....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenomedullin; Hypertension; Heart failure; Aldosterone; Renin; Norepinephrine.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800016

Chronic hypertension alters the expression of Cx43 in cardiovascular muscle cells
Provedor de dados: 56
Autores: Haefliger,J.-A.; Meda,P.
Connexin43 (Cx43), the predominant gap junction protein of muscle cells in vessels and heart, is involved in the control of cell-to-cell communication and is thought to modulate the contractility of the vascular wall and the electrical coupling of cardiac myocytes. We have investigated the effects of arterial hypertension on the expression of Cx43 in aorta and heart in three different models of experimental hypertension. Rats were made hypertensive either by clipping one renal artery (two kidney, one-clip renal (2K,1C) model) by administration of deoxycorticosterone and salt (DOCA-salt model) or by inhibiting nitric oxide synthase with NG-nitro-L-arginine methyl ester (L-NAME model). After 4 weeks, rats of the three models showed a similar increase in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell-cell communication; Gap junctions; Connexins; Vasculature; Heart; Hypertension; Blood pressure; Renin; Deoxycorticosterone; Nitric oxide; Distensibility.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400009

Dissociation between the circulating renin-angiotensin system and angiotensin II receptors in central losartan-induced hypertension
Provedor de dados: 56
Autores: Sugawara,A.M.; Vendramini,R.C.; Barbosa,S.P.; Brunetti,I.L.; Menani,J.V.; De Luca Jr.,L.A.
Losartan, an AT1 angiotensin II (ANG II) receptor non-peptide antagonist, induces an increase in mean arterial pressure (MAP) when injected intracerebroventricularly (icv) into rats. The present study investigated possible effector mechanisms of the increase in MAP induced by icv losartan in unanesthetized rats. Male Holtzman rats (280-300 g, N = 6/group) with a cannula implanted into the anterior ventral third ventricle received an icv injection of losartan (90 µg/2 µl) that induced a typical peak pressor response within 5 min. In one group of animals, this response to icv losartan was completely reduced from 18 ± 1 to 4 ± 2 mmHg by intravenous (iv) injection of losartan (2.5-10 mg/kg), and in another group, it was partially reduced from 18 ± 3 to 11 ± 2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin II; Alpha1-Adrenoceptors; Arterial pressure; AT1 receptors; Angiotensin-converting enzyme; Renin.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900007

Reduced plasma levels of angiotensin-(1-7) and renin activity in preeclamptic patients are associated with the angiotensin I- converting enzyme deletion/deletion genotype
Provedor de dados: 56
Autores: Velloso,E.P.; Vieira,R.; Cabral,A.C.; Kalapothakis,E; Santos,R.A.S.
The relationship between preeclampsia and the renin-angiotensin system (RAS) is poorly understood. Angiotensin I-converting enzyme (ACE) is a key RAS component and plays an important role in blood pressure homeostasis by generating angiotensin II (Ang II) and inactivating the vasodilator angiotensin-(1-7) (Ang-(1-7)). ACE (I/D) polymorphism is characterized by the insertion (I) or deletion (D) of a 287-bp fragment, leading to changes in ACE activity. In the present study, ACE (I/D) polymorphism was correlated with plasma Ang-(1-7) levels and several RAS components in both preeclamptic (N = 20) and normotensive pregnant women (N = 20). The percentage of the ACE DD genotype (60%) in the preeclamptic group was higher than that for the control group (35%);...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renin-angiotensin system; Angiotensin-converting enzyme; Renin; Angiotensin-(1-7); ACE polymorphism (I/D); Preeclampsia.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400018