Consulta: subjectFacets:"SDS-PAGE"
Registros recuperados: 33
Data/hora: 09/06/2024 15:14:04

Senescencia foliar, componentes del rendimiento y degradación nuclear en dos variedades de papa (Solanum tuberosum L.) var. Lupita y Tollocan.
Provedor de dados: 32
Autores: Zárate Castrejón, José Luis.
La senescencia foliar es la última etapa del desarrollo de la hoja, se caracteriza por una serie de cambios fisiológicos, bioquímicos y estructurales. Las hojas pierden gradualmente clorofila, se desorganiza la maquinaria fotosintética y se reciclan los productos de degradación de las macromoléculas hacia otras partes en crecimiento (hojas jóvenes, flores, frutos) u órganos de almacenamiento como el tubérculo en Solanum tuberosum. Se conoce poco acerca de la relación entre la senescencia foliar y proceso del llenado del tubérculo. El objetivo del presente trabajo fue estudiar la senescencia foliar en dos variedades de papa con diferente ciclo biológico y relacionarlo con el llenado del tubérculo. Se monitoreó el amarillamiento foliar en toda la planta a...
Palavras-chave: ADN; Cloroplastos; LSU; Rubisco; SDS-PAGE; Cloroplast; DNA; Maestría; Botánica.
Ano: 2010
URL: http://hdl.handle.net/10521/275

Assessment of genetic diversity among Pakistani wheat (Triticum aestivum L.) advanced breeding lines using RAPD and SDS-PAGE
Provedor de dados: 69
Autores: Ahmed,Muhammad Farooq; Iqbal,Muhammad; Masood,Muhammad Shahid; Rabbani,Malik Ashiq; Munir,Muhammad.
Genetic diversity was assessed among 32 advanced wheat breeding lines included in the National Uniform Wheat Yield Trials (2006-07) of Pakistan using molecular (DNA) and biochemical (SDS-PAGE) markers. Of the 72 RAPD primers used for initial screening, 15 were found polymorphic. A total of 140 bands (61.4% polymorphic) were generated by the 15 random decamer primers. Genetic similarity coefficients ranged from 0.81 to 0.94 for rainfed and from 0.70 to 0.93 for the normal seeding date group. Cluster analysis using the unweighted pair group method of arithmetic averages (UPGMA) clustered the 32 advanced wheat breeding lines into one major and three small groups. Maximum level of polymorphism (90%) was observed for the primer OPA-05. Lines N9 and N11 showed...
Tipo: Journal article
Palavras-chave: Genetic diversity; Molecular markers; Polymorphism; RAPD; SDS-PAGE; Wheat.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300001

Estimation of phylogenetic relationships of Phlomis species based on seed protein polymorphism
Provedor de dados: 69
Autores: Yüzbasioglu,Ertugrul; Dadandi,Mehmet Yasar; Özcan,Servet.
In this study, phylogenetic relationships among 39 Phlomis taxa were investigated based on seed protein profiles produced by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). A total of 21 polypeptide bands were scored, of which, 19 were polymorphic among the taxa of the genus Phlomis. A distance matrix was generated from the similarity matrix which was computed by using Jaccard&amp;#8217;s similarity coefficients, based on polymorphic bands and then an UPGMA tree was established through cluster analysis performed on the distance matrix. Genetic distances ranged from 0.00 to 0.50 within subsection Dendrophlomis; from 0.00 to 0.625 within subsection Gymnophlomis and from 0.00 to 0.769 within subsection Oxyphlomis. The UPGMA tree formed...
Tipo: Journal article
Palavras-chave: Phlomis; Phlomoides; Phlomis taxa; SDS-PAGE; Seed proteins.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200002

Adaptação de método de extração e caracterização de proteínas em bactéria de importância para a indústria alimentícia.
Provedor de dados: 14
Autores: STEPHAN, M. P.; TEIXEIRA, K. R. dos S.
bitstream/item/164076/1/CT113-extracao-proteinas.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: SDS-PAGE; Método de extração.; Eletroforese; Proteína.
Ano: 2007
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/413478

Caracterización de las inmunoglobulinas del ñandú (Rhea americana)
Provedor de dados: 78
Autores: Montagna,D.R.; Rigo,V; Ramayo,L.G.; Goldman,L.H.; Huguet,M.J.; Maceira,N; Jar,A.M.
El objetivo de este trabajo fue caracterizar las inmunoglobulinas séricas y la IgY de la yema de huevo de ñandú, a través de las técnicas de electroforesis en acetato de celulosa, electroforesis en gel de poliacrilamida y Western-blot. Se estudiaron 19 ñandúes clínicamente sanos de 14 a 15 meses de edad, provenientes un criadero de Balcarce, Argentina. La electroforesis en acetato de celulosa de los sueros mostró la presencia de cuatro fracciones proteicas: albúmina, alfa, beta y gamma globulinas. La concentración de las gamma globulinas fue de 0,69 ± 0,18 g/dl, con un rango de 0,42-1,05 g/dl. La corrida electroforética de la IgY muestró que esta se ubica en la fracción gammaglobulínica. La electroforesis en gel de poliacrilamida evidenció, tanto en suero...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ã‘andú; Electroforesis; SDS-PAGE.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982013000200002

THE EFFECT OF A POLYDNAVIRUS OF APANTELES GALLERIAE  ON THE HEMOLYMPH PROTEINS OF HOST GALLERIA MELLONELA
Provedor de dados: 79
Autores: BROCHETTO-BRAGA,M.R.; PALMA,M.S.; RIBEIRO,J.C.; GOBBI,N.
The presence of a polydnavirus in the ovary of the microhymenoptera Apanteles galleriae was detected. This wasp parasites the larvae of the wax moth Galleria mellonella. To study the possible viral effects on the total protein of G.mellonella, hemolymph extracts of the ovary calyxes of parasitized A. galleriae were injected into 6th and 7th instar cells of G.mellonella larvae. Protein profiles on polyacrilamide gel electrophoresis containing sodium dodecyl sulfate (SDS-PAGE) of host cell extracts showed a suppression of several protein bands when infection occurred in the 6th instar. However, in the 7th instar, parasitism caused an amplification in the synthesis of most proteins with some changes in the electrophoretic profile.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microhymenoptera; Wax moth; Parasitism; Polydnavirus; SDS-PAGE; Apanteles galleriae; Galleria mellonela.
Ano: 1995
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301995000200005

Intraspecific variation in protein pattern of red scorpion (Mesobuthus tamulus, coconsis, pocock) venoms from Western and Southern India
Provedor de dados: 80
Autores: Badhe,R. V.; Thomas,A. B.; Harer,S. L.; Deshpande,A. D.; Salvi,N.; Waghmare,A.
Red scorpions Mesobuthus tamulus (Coconsis, Pocock) were obtained from different regions of West and South India (Ratnagiri, Chiplun and Ahmednagar from Maharashtra and Chennai from Tamil Nadu, respectively). Their venoms composition was analyzed using gel electrophoresis (SDS-PAGE). All venom samples shared six bands of 170, 80, 60, 57, 43, and 38 kDa molecular weights. Bands of 115 kDa and 51.5 kDa were characteristic of venoms obtained from red scorpions of Chiplun region, and the 26kDa band was absent in scorpion venom from Tamil Nadu. The separated protein band patterns suggest that the venoms from Ratnagiri, Ahmednagar and Tamil Nadu had high similarities in their biochemical composition but differed from that of Chiplun region. These data were also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Red scorpion; SDS-PAGE; Mesobuthus tamulus.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400008

Protein profile analysis of Malaysian snake venoms by two-dimensional gel electrophoresis
Provedor de dados: 80
Autores: Vejayan,J; Shin Yee,L; Ponnudurai,G; Ambu,S; Ibrahim,I.
Snake venoms comprise a highly complex mixture of proteins, which requires for their characterization the use of versatile two-dimensional electrophoresis techniques. In the present study, venoms obtained from eight snakes (Ophiophagus hannah, Naja kaouthia, Naja sumatrana, Bungarus fasciatus, Trimeresurus sumatranus, Tropidolaemus wagleri, Enhydrina schistosa and Calloselasma rhodostoma) commonly found in Malaysia were separated based on two independent properties, isoelectric point (pI) and molecular weight (MW). Many differences in snake venoms at the inter-family, inter-subfamily, inter-genus and inter-species levels were revealed. Notably, proteins from individuals of the Viperidae family - Trimeresurus sumatranus, Tropidolaemus wagleri and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Protein profile analysis; Two-dimensional gel electrophoresis; SDS-PAGE.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400013

Haemolymph electrophoretic pattern of Ascia monuste orseis larvae (Lepidoptera: pieridae) parasitized by Cotesia glomerata (Hymenoptera: braconidae)
Provedor de dados: 80
Autores: Scaglia,M.; Brochetto-Braga,M. R.; Chaud-Netto,J.; Gobbi,N.
Cotesia glomerata is a natural enemy of the vegetable plague Ascia monuste orseis and preferably parasites 2nd, 3rd and 4th instar larvae. Parasitism effects on the haemolymph protein profile of Ascia monuste orseis larvae from the 2nd to 7th days were analyzed both qualitatively and quantitatively by SDS-PAGE and Coomassie-Blue binding methods. Quantitative analysis showed a progressive increase in the protein content of about 6.5 and 12.5 times in parasitized and non-parasitized larvae from the 2nd to 5th days, respectively. On the 6th day, a decrease in protein content was observed in both groups, although this decrease was significantly less than the control group that continued to metamorphosis. Meanwhile, parasitized larvae had one more day (7th day)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascia monuste orseis; Cotesia glomerata; Electrophoretic protein profile; Haemolymph; Natural enemy; Parasitism; SDS-PAGE.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100006

Comparison of protein profile of co-existing Fasciola hepatica and Fasciola gigantica parasite in Bos taurus (cattle) and Bubalus bubalis (Philippine water buffalo)
Provedor de dados: 19
Autores: De Vera, M.E; Sato, K; Oyong, G; Claveria, F.G.
Fasciola hepatica and Fasciola gigantica habitually co-exist as parasites of cattle (Bos taurus) and water buffalo (Bubalus bubalis), and despite variation in their morphometry, their species status is suspicious. Liver flukes isolated from cattle and water buffalo were initially sorted as F. gigantica or F. hepatica, and crude proteins were extracted and subjected to SDS-PAGE. Cattle had the preponderance of F. gigantica, while F. hepatica was the dominant species in water buffaloes. Co-existing cattle and bubaline fasciolids revealed similar protein profile suggestive of a close genetic relationship. The distinct heavy bands shared by co-existing bubaline F. hepatica and F. gigantica relative to those detected in cattle fasciolids suggests a host...
Palavras-chave: Cattle &amp; carabao Fasciola spp; Protein profile; SDS-PAGE; Philippines.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2382

Extração e análise eletroforética em gel de poliacrilamida (SDS-PAGE) de proteínas totais de folhas e raízes de Piper tuberculatum
Provedor de dados: 36
Autores: Silva,Redinaldo dos Santos; Souza,Cláudia Regina Batista de.
O Estado do Pará é o principal produtor brasileiro de pimenta-do-reino (Piper nigrum Link), entretanto a sua produção tem sido bastante afetada pela doença conhecida como fusariose. O Fusarium solani f. sp. piperis é o agente causador desta doença que afeta o sistema radicular da planta, causando o apodrecimento das raízes e a queda das folhas levando à morte da planta. Algumas piperáceas nativas da região amazônica, entre elas a espécie Piper tuberculatum Jacq., têm se mostrado resistentes à infecção pelo F. solani f. sp. piperis, e desta forma têm sido utilizadas em estudos de interação planta-patógeno. Neste trabalho foram avaliadas cinco condições de extração de proteínas com o objetivo de selecionar tampões adequados para a extração de proteínas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fusariose; Fusarium solani f. sp. piperis; Piper nigrum; Piper tuberculatum; Proteômica; SDS-PAGE.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672009000200002

SDS-PAGE detection of a specific marker in ‘Fécula Branca’ cassava cultivar, Manihot esculenta Crantz (Euphorbiaceae)
Provedor de dados: 3
Autores: Pereira, Álvaro Júlio; UEM; Collet, Sandra Aparecida de Oliveira; UEM; Vidigal Filho, Pedro Soares; UEM; Machado, Maria de Fátima Pires da Silva; Biologia - UEM.
Proteins from young unexpanded leaves of seven cassava cultivars Manihot esculenta, Crantz (Euphorbidaceae) were investigated through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The comparison was made through the protein patterns obtained, and their relative amounts determined in plants leaves infected or not with the bacteria Xanthomonas axonopodis pv. manihotis. The electrophoretic protein pattern obtained from the investigated cultivars (“Fibra”, “Fécula Branca”, “Branca de Santa Catarina”, “Verdinha”, “IAC-12”, “IAC-13”, and “IAC-14”) showed a polypeptide subunit present exclusively in the “Fécula Branca” cultivar, with a molecular weight of 93.5 kDa. Also, protein fractions were more intensely stained in young leaves of M....
Palavras-chave: 2.00.00.00-6 Ciências Biológicas cultivares de mandioca; Proteínas; SDS-PAGE; Patogênese em mandioca 2.00.00.00-6 Ciências Biológicas.
Ano: 2002
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2365

Comparative electrophoretic profile of proteins and esterases in healthy silkworm larvae (Bombyx mori Lineu, 1758) and infected with nucleopolyhedrovirus - doi: 10.4025/actascibiolsci.v35i1.8612
Provedor de dados: 3
Autores: Ruiz, Juliana Bueno; Universidade Estadual de Maringá; Ronqui, Ludimilla; Universidade Federal de Rondonia; Oliveira, Jussara Ricardo de; Universidade Paranaense; Ruvolo-Takasusuki, Maria Claudia Colla; Universidade Estadual de Maringá.
Total proteins and esterases from silk gland extracts of Bombyx mori silkworm were characterized and compared with electrophoretic profiles of prepared extracts with silkworm glands infected with nucleopolyhedrovirus (BmNPV). SDS-PAGE (7%) gels was used for the total proteins, and 10% native PAGE for esterases. In the silk glands extracts of healthy silkworms, it was observed seven protein zones with molecular weight varying between 10 kDa (P1) and 60 kDa (P7). In the infected silkworms, a new zone named P8 (90 kDa) was also detected. Esterases activity at 5th instar larvae underwent changes after the infection with BmNPV, since there was a reduction (EST-6 and EST-7) and an increase (EST-8) in the intensity of the regions of esterases activity, and...
Tipo: Genética Bioquímica
Palavras-chave: 2.02.04.00-0 viral infection; Silk gland; Isoenzymes; SDS-PAGE; Molecular markers 2.02.02.00-8.
Ano: 2012
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8612

Characterization of onion (Allium cepa L.) varieties based on physical properties and seed performance
Provedor de dados: 115
Autores: Herrera-Corredor,Carlos; Carrillo-Castañeda,Guillermo.
Abstract: Five cultivars (Cristal White Wax, Toro, Red Burgundy, Cojumatlán and Contessa) of onion (Allium cepa L.) were characterized according to: the weight of 100 seeds, germination kinetics, speed germination (T50) and seed protein analysis. Seed of Crystal White Wax was differentiated from the other cultivars by its low weight (0.30 g), since the weight of the rest was 0.40 g. Crystal White Wax had low seed germination (56%) and the highest delay of germination (T50 values were 78 h), while T50 of the rest was 52 h. Protein was isolated from seeds at the precise moment of seed germination. All cultivars were differentiated by their pattern and number of proteins resolved by SDS-PAGE electrophoresis. Toro and Red Burgundy showed the greater difference...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa L.; Germination kinetics; SDS-PAGE; T50; Vigor.
Ano: 2007
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952007000700755

Growth, Yield and Grain Nutritional Quality in Three Brazilian Pearl Millets (Pennisetum americanum L.) with African or Indian origins
Provedor de dados: 42
Autores: MEDICI,LEONARDO O.; GONÇALVES,FABÍOLA V.; FONSECA,MARCOS PAULO S. DA; GAZIOLA,SALETE A.; SCHMIDT,DAIANA; AZEVEDO,RICARDO A.; PIMENTEL,CARLOS.
ABSTRACT In this study, we are presenting recommendations to the best agricultural use as well as for plant breeding of three millet cultivars namely ENA1 and ENA2, which have African origin, and BRS1501 originally from India. These cultivars were evaluated for growth, yield and grain quality traits. The morphological traits evaluated in this study indicated that the African genotypes ENA1 and ENA2 are better than the Indian genotype BRS1501 for no-till farming or to produce forage with 15% of crude protein at flowering and at harvest to produce stover (around 7% of crude protein content) for livestock feeding. The BRS1501 cultivar exhibited the highest values for total crude protein, albumins and prolamins, phytate and mineral contents in grains. ENA1 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mineral density; SDS-PAGE; Phytate; Seed storage proteins; Amino acids.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401749

Proteinograma sérico de bezerros recém-nascidos da raça Holandesa obtido por eletroforese em gel de poliacrilamida
Provedor de dados: 45
Autores: Fagliari,J.J.; Rizolli,F.W.; Silva,S.L.; Silva,D.G.
The serum protein concentration of newborn Holstein calves determined by means of sodium dodecyl sulphate-polyacrylamide (SDS-PAGE) was studied. Blood samples from 40 healthy newborn calves were obtained 48 hours after birth. Calves had been given 3 liters of colostrum within 2 hours after birth, following by dose corresponding by 15% of animal weight each 24 hours. The results showed three different proteinograms: 19 calves had 14 proteins with molecular weights (MW) ranging from 28,000 D to 170,000D (proteinogram 1); 11 calves had 14 proteins with MW ranging from 18,000 to 170,000 D (proteinogram 1); and 10 calves had 12 proteins with MW ranging from 28,000 D to 170,000 D (proteinogram 3). The three groups presented similar IgG levels. The highest serum...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bezerro; Proteinograma; Eletroforese; SDS-PAGE.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300026

Proteinograma sérico de bovinos da raça Curraleir
Provedor de dados: 45
Autores: Juliano,R.S.; Fioravanti,M.C.S.; Fagliari,J.J.; Silva,P.C.; Silva,L.A.F.
Estudou-se o perfil eletroforético das proteínas séricas de bovinos sadios da raça Curraleiro por meio da técnica de eletroforese em gel de acrilamida contendo dodecil sulfato de sódio. Utilizaram-se amostras de soro sanguíneo de 228 bovinos da raça Curraleiro, 51 machos e 177 fêmeas, com idades entre sete meses e 12 anos, pertencentes a dois rebanhos localizados nos Estados de Goiás e Tocantins. Foram quantificadas proteína total e concentração plasmática de fibrinogênio. Verificaram-se variações nas concentrações das diferentes frações proteicas. Foram detectadas 26 proteínas e identificadas 10 delas. A ceruloplasmina esteve ausente em 78,1% dos indivíduos, e a α-antitripsina não foi detectada em nenhum animal. Proteína total, globulina, IgA, IgG e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Raça nativa; SDS-PAGE; Proteína; Fibrinogênio.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000300002

Morfometria corpórea, características do sêmen, proteínas seminais e testosterona em cervos Cervus unicolor, em cativeiro
Provedor de dados: 45
Autores: Chacur,M.G.M.; Martins,E.A.F.; Silva,A.A.; Guaberto,L.M.; Gabriel Filho,L.R.A.; Sanches,O.C.; Oba,E.
Objetivou-se estudar a morfometria corpórea, as características do sêmen, o perfil proteico do plasma seminal em SDS-PAGE e a concentração sérica de testosterona em cervos-sambar (Cervus unicolor), criados em cativeiro, na estação reprodutiva da primavera. Quatro machos com idades entre 12 e 36 meses foram avaliados em quatro momentos, com intervalos de sete dias, com peso corpóreo (60,5 a 89,0kg), índice de massa corporal (93,07kg/m2 a 126,56kg/m2), volume do ejaculado (0,50±0,35mL a 0,75±0,28mL), motilidade espermática (87,75±4,78% a 90,00±7,07%), defeitos totais (17,25±5,81% a 47,72±17,55%), testosterona sérica (6,43±4,33ng/dL a 166,00±64,48ng/dL) e proteínas do plasma seminal com bandas entre 7,6 e 142kDa. As características dos ejaculados não...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cervidae; Cativeiro; Sêmen; SDS-PAGE; Andrógeno.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000501392

Proteinograma sérico de veados-catingueiro (Mazama gouazoubira) criados em cativeiro obtido por eletroforese em gel de agarose e de poliacrilamida (SDS PAGE)
Provedor de dados: 45
Autores: Santana,A.M.; Fagliari,J.J.; Camargo,C.M.S.; Santana,A.E.; Duarte,J.M.B.; Silva,P.R.L.
The serum protein concentrations of brown brocket deer (Mazama gouazoubira) obtained by agarosis gel and sodium dodecyl sulphate-polyacrylamide (SDS-PAGE) gel were determined from blood samples of ten adult healthy animals (six females and four males), monthly collected in the morning, during 12 months. The animals, maintained in individual stable and protected from noise, received ad libitum a diet of comercial ration and green roughage. Serum protein concentrations in agarosis gel revealed the presence of four protein fractions: albumin, alphaglobulin, betaglobulin, and gammaglobulin. Only serum concentrations of albumin were influenced by season, being values in spring higher than values in summer (4.15 x 3.64g/dl). Serum concentrations of albumin (4.05...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Veado-catingueiro; Proteinograma; Eletroforese; SDS-PAGE.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000600039

Transferrina como marcador de maturação pulmonar em cordeiros nascidos a termo ou prematuros
Provedor de dados: 45
Autores: Baptista,R.S.; Bovino,F.; Denadai,D.S.; Rahal,N.M.; Perri,S.H.V.; Lucas,F.A.; Feitosa,F.L.F.; Peiró,J.R.; Mendes,L.C.N.
RESUMO O objetivo do estudo foi procurar proteínas de fase aguda que possam indicar sinais de maturação no neonato prematuro, por meio da quantificação sérica delas. Identificou-se a imunoglobulina A, a ceruloplasmina, a haptoglobina, a glicoproteína ácida, a transferrina, a albumina e as imunoglobulinas G de cadeias leve e pesada, pela comparação do perfil dos proteinogramas de cordeiros nascidos a termo com os prematuros submetidos a diferentes protocolos terapêuticos, a fim de estimular a atividade respiratória. Constituíram-se seis grupos: PN (n= 9): nascidos de parto normal; CN (n= 7): nascidos de cesariana em tempo normal de gestação; CP (n= 6): nascidos de cesariana prematura sem nenhum tipo de tratamento; DEX (n= 9): prematuros cujas mães receberam...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovinos; Neonatologia; Eletroforese; SDS-PAGE; Proteinograma.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000401299

Screening of Colletotrichum (Ascomycota) isolates, causal agents of Soybean Anthracnose, for Laccase production
Provedor de dados: 51
Autores: Levin,L.; Ramos,A .M.; Parisi,M .; Gally,M.
Colletotrichum truncatum is the most common pathogen fungus associated with soybean anthracnose. Although the lignin-degrading enzyme laccase has been implicated in pathogenicity of a wide range of plant pathogenic fungi, its biological role in the Colletotrichum -soybean disease system is unknown. The extent of the infection in our country led us to examine laccase production in Argentinean Colletotrichum strains isolated from diseased soybean plants from different geographic locations. Ten strains (eight of them identified as C. truncatum) , were screened for in vitro laccase production. Only six of the isolates, all of them C. truncatum , produced laccase activity when cultured on a defined medium based on pectin and asparagine as carbon and nitrogen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anthracnose; Colletotrichum; Laccase; SDS-PAGE; Soybean.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722007000100010

Role of Light and Dark Cycle and Different Temperatures in the Regulation of Growth and Protein Expression in Oscillatoria agardhii Strain
Provedor de dados: 52
Autores: Dautania,Gajendra Kumar; Singh,Gajendra Pal.
The cyanobacterium Oscillatoria agardhii was isolated from the fresh water Mawatha lake, Jaipur and was grown in Zarrouk's medium at 25 ± 2°C, illuminated with white fluorescent light at the intensity of 2 500 lux with 12:12 h light and dark photoperiod. The effects of photoperiod and temperature on the growth and protein expression by Oscillatoria agardhii were studied under different controlled culture conditions (ALR, ALC, CLR, CLC, and NDL), measuring optical density, cell count and dry weight. Protein content was measured quantitatively by Bradford assay and qualitatively by SDS-PAGE. The densitometric analysis was also carried out for the measurement of the expression level of different proteins/peptides under different culture conditions. Maximum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bradford assay; Densitometer; Oscillatoria agardhii; Photoperiod; SDS-PAGE.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600933

Detection of Genetic Relationship in the Tree of Life of Some Quail Through Molecular Markers Analyses
Provedor de dados: 52
Autores: Deef,Lamiaa Elsayed Mokhtar.
ABSTRACT  Quail is an important and interesting group of galliform birds. The Common quail (Coturnix coturnix); the Japanese quail (Coturnix japonica); the Panda quail (Coturnix japonica); the Dotted white quail (Coturnix japonica) and the Bobwhite quail (Colinus virginianus) were used in this study. PCR-RFLP and SDS-proteins were performed to reveal the genetic characterization and genetic relationship of the studied quails. Analysis of fragments generated by digestion of PCR product with restriction enzyme NlaIII recorded highly polymorphic restriction profiles. There is a wide intraspecific COI, SEMA3E and TLX genes variability among the studied quails. Protein bands varied from10 to 18 between quails with minimum number of bands were in the Dotted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Birds; PCR; Quail; SDS-PAGE; Galliformes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100425

Rhizosphere Bacterial Composition of the Sugar Beet Using SDS-PAGE Methodology
Provedor de dados: 52
Autores: Aeini,Milad; Khodakaramian,Gholam.
ABSTRACT The rhizosphere zone has been defined as the volume of soil directly influenced by the presence of living plant roots or soil compartment influenced by the root. During the growing season of 2014, the rhizobacteria of 23 sugar beet plants sampled from 12 sites in the west and north west of Iran were inventoried. Using a cultivation-dependent approach, a total of 217 bacteria were isolated from the rhizosphere. The bacterial isolates were tentatively grouped and documented based on polyacrylamide gel electrophoresis of whole-cell proteins and were found to represent 43 different protein electrotypes. The majority of the fingerprint types were found only on a single occasion. Fifty-nine percent of the strains belonged to the five bacterial species...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhizosphere; Sugar beet; SDS-PAGE; Protein fingerprint.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100451

Detection of the basement membrane-degrading proteolytic activity of Paracoccidioides brasiliensis after SDS-PAGE using agarose overlays containing Abz-MKALTLQ-EDDnp
Provedor de dados: 56
Autores: Puccia,R.; Juliano,M.A.; Juliano,L.; Travassos,L.R.; Carmona,A.K.
We have characterized, in the Paracoccidioides brasiliensis yeast phase, an exocellular SH-dependent serine proteinase activity against Abz-MKRLTL-EDDnp and analogous fluorescent-quenched peptides, and showed that it is also active against constituents of the basement membrane in vitro. In the present study, we separated the components of P. brasiliensis culture filtrates by electrophoresis and demonstrated that the serine-thiol exocellular proteinase has a diffuse and heterogeneous migration by SDS-PAGE, localizing in a region between 69 and 43 kDa. The hydrolytic activity was demonstrable after SDS-PAGE using buffered agarose overlays of Abz-MKALTLQ-EDDnp, following incubation at 37oC, and detection of fluorescent bands with a UV transilluminator....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P. brasiliensis; Serine-thiol proteinase; SDS-PAGE; Fluorescent-quenched peptides.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500019

Characterization of cry2-type genes of Bacillus thuringiensis strains from soil-isolated of Sichuan basin, China
Provedor de dados: 58
Autores: Liang,Hongxia; Liu,Yao; Zhu,Jun; Guan,Peng; Li,Shuangcheng; Wang,Shiquan; Zheng,Aiping; Liu,Huainian; Li,Ping.
Sichuan basin, situated in the west of China, is the fourth biggest basin in China. In order to describe a systematic study of the cry2-type genes resources from Bacillus thuringiensis strains of Sichuan basin, a total of 791 Bacillus thuringiensis strains have been screened from 2650 soil samples in different ecological regions. The method of PCR-restriction fragment length polymorphism (PCR-RFLP) was used to identify the type of cry2 genes. The results showed that 322 Bacillus thuringiensis strains harbored cry2-type genes and four different RFLP patterns were found. The combination of cry2Aa/cry2Ab genes was the most frequent (90.4%), followed by cry2Aa (6.8%) and cry2Ab alone (2.5%), and only one novel type of cry2 gene was cloned from one isolate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus thuringiensis; PCR-RFLP; SDS-PAGE; Novel cry2-type gene.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100018

SDS-Page and numerical analysis of Candida albicans from human oral cavity and other anatomical sites
Provedor de dados: 58
Autores: Rodrigues,Cristina Crespo; Höfling,José Francisco; Boriollo,Marcelo Fabiano Gomes; Rodrigues,Janaina Aparecida de Oliveira; Azevedo,Ricardo Antunes de; Gonçalves,Reginaldo Bruno; Gomes,Luiz Humberto; Tavares,Flávio César Almeida.
The aim of the present research was to evaluate the protein polymorphism degrees among forty-eight C. albicans isolates from fourteen anatomical sites of clinical patients by polyacrylamide gel electrophoresis (SDS-PAGE) and numerical analyzes, in order to identify subspecies and their similarities in some infectious niches. Cell cultures were grown in YEPD medium, collected by centrifugation, and washed in cold saline solution. The whole-cell proteins were extracted by cell disruption using glass beads and submitted to SDS-PAGE technique. After electrophoresis, the protein bands were stained with coomassie-blue and analyzed by statistics package NTSYS-pc version 1.70 software. Similarity matrixes and dendrograms were generated by application of similarity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Numerical analyzes; SDS-PAGE; C. albicans; Human anatomical sites.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100006

Comparison of electrophoretic protein profiles of Campylobacter jejuni subsp. jejuni isolated from different animal species
Provedor de dados: 58
Autores: Scarcelli,Eliana; Costa,Elizabeth Oliveira da; Genovez,Margareth Élide; Cardoso,Maristela Vasconcellos; Bach,Erna Elizabeth; Torres,Ana Paula.
Electrophoretic protein profiles of Campylobacter jejuni subsp. jejuni strains isolated from feces of seven animal species, including man, were compared. Fourteen strains (two from each species) plus two human strains and the reference one, were ruptured by ultrasound and their total soluble proteins were analyzed by SDS-PAGE technique in a 12% polyacrylamide gel with computerized densitometric reading by the molecular analyst software. All the strains had bands in common that correspond to 45 and 66 Kda molecular weight. The disagreement corresponded to a 97 to 200 Kda molecular weight region. From the 17 strains, 13 (76.5%), were classified as biotype I, three (17.6%) as biotype II and one (5.8%) as biotype III. Since protein extracts were obtained from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Campylobacter jejuni; SDS-PAGE; Biotyping.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400006

Improved adsorption-desorption extraction applied to the partial characterization of the antilisterial bacteriocin produced by Carnobacterium maltaromaticum C2
Provedor de dados: 58
Autores: Tulini,F. L; De Martinis,E.C.P.
Bacteriocins are ribosomally produced peptides useful for food biopreservation. An improved adsorption-desorption process is proposed for the partial purification of the bacteriocin produced by the fish isolate Carnobacterium maltaromaticum C2. Analyzis of extract by SDS-PAGE indicated this method may offer an alternative to improve the yield of purification of bacteriocins.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteriocins; LAB; Carnobacterium; SDS-PAGE.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200032

Protein profile analysis by SDS-PAGE of Mycoplasma Gallisepticum strains S6(208) and F-K810 grown in Hayflick's and Frey's media
Provedor de dados: 58
Autores: Ferraz,Priscila N.; Menezes,Claudio C. P.; Danelli,Maria das Graças M.; Lizeu,Jackeline de O. P.; Nascimento,Elmiro R. do; Lucchesi,Daniela M.
The Mycoplasma gallisepticum strains [wild-type S6 (208) and a vaccine-type F-K810] grown in Frey´s and Hayflick´s media were analyzed by SDS-PAGE. No visual changes in the protein profiles of these strains were observed regardless of the media composition used, although the polyacrylamide gel electrophoretograms showed minor differences do exist when densitometer traces of the gel are compared. Both strains were easily differentiated on SDS-PAGE analysis by a peptide band p75, that is specific for MG F-K810 strain, used as vaccine.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mycoplasma gallisepticum; SDS-PAGE; Protein profile.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200010

Molecular analysis of endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.)
Provedor de dados: 58
Autores: Figueiredo,José Edson Fontes; Gomes,Eliane Aparecida; Guimarães,Claudia Teixeira; Lana,Ubiraci Gomes de Paula; Teixeira,Marta Aparecida; Lima,Guilherme Vitor Corrêa; Bressan,Wellington.
Endophytic bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from Brazilian sweet corn. SDS-PAGE of wholecell protein extract of fortytwo isolates revealed a high number of scrutinable bands. Twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique. Some high-accumulated polipeptides with variable length were observed in almost isolates. Partial sequencing of 16S ribosomal gene revealed that all isolates are Bacillus sp. and among thirteen isolates with similar protein profiles, two were different strains....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endophytic bacteria; Bacillus; Sweet corn; SDS-PAGE; RDNA sequencing.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300014

Retenção de placenta no proteinograma de vacas Holandesas
Provedor de dados: 65
Autores: Saut,João Paulo Elsen; Miyashiro,Samantha Ive; Raimondo,Raquel Fraga e Silva; Nunes,Mariana Tikuma; Mori,Clara Satsuki; Fagliari,Jose Jurandir; Birgel Junior,Eduardo Harry.
Com o objetivo de avaliar a influência da retenção de placenta (RP) no proteinograma de fêmeas bovinas da raça Holandesa, de propriedades comerciais, foram utilizadas 129 vacas com RP e 145 vacas com parto e pós-parto fisiológicos e sem nenhum tratamento no período avaliado. As amostras de sangue foram divididas nos momentos: 1odia pós-parto (DPP), 2o-3o, 4o-5o, 6o-7o, 8o-14o, 15o-29o, 30o-59o e 60o-90o DPP. O fracionamento das proteínas foi realizado por eletroforese em fita de acetato de celulose e em gel de poliacrilamida, contendo dodecil sulfato de sódio (SDS-PAGE), nas quais se avaliou o comportamento de 19 bandas proteicas identificadas pelos respectivos pesos moleculares, que variaram entre 23KDa e 187KDa. Não houve influência da RP na proteína...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovinos; Puerpério; Proteínas de fase aguda; SDS-PAGE.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000901651

Growth characteristics and dynamics of protein synthesis in callus cultures from Glycine wightii (Wight &amp;amp; Arn.) Verdc.
Provedor de dados: 64
Autores: Silva,André Luis Coelho da; Caruso,Cecília Sulzbacher; Moreira,Renato de Azevedo; Horta,Ana Cecília Góes.
Cotyledon explants were first cultured on MS medium supplemented with 4.52 M 2,4-D and 0.46 mM kinetin. The development of the calli was followed (0, 4, 8, 12, 16, 20, 24, 28 and 32 days after) and the growth curve was determined, based in fresh and dry weight. The growth curve presented sigmoidal form with four distinct phases. The highest growth percentage was observed at the exponential phase and the lowest at the stationary phase. These results indicated that cotyledon callus subculture should be performed 20 days after inoculation. The calli obtained after a period of 28 days were freeze dried, macerated and submitted to extraction with buffers of different pH values (2.6; 4.0; 6.0; 8.0 and 10.0) and the proteins in the extracts were determined by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine; Leguminosae; Callogenesis; SDS-PAGE.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542005000600009