Consulta: subjectFacets:"Saccharomyces cerevisiae"
Registros recuperados: 128
Data/hora: 12/06/2024 15:05:20

ENERGY CULTURES AND SUSTAINABILITY IN BIOFUEL PRODUCTION
Provedor de dados: 196
Autores: Mascarenhas Santos, Maria do Socorro; Silva, Cesar José da; Cruz, Sandra Helena da; Batistote, Margareth; Cardoso, Claudia Andrea Lima.
Energy cultures are emerging as viable renewable energy sources because they satisfy sustainability requirements. The present study involves a survey regarding the technological characteristics for sugarcane and sweet sorghum energy crops, in addition to their potential for bioethanol production. An exploratory survey was conducted regarding the agronomic and technological characteristics of cane and sorghum. Pre-inmates were produced with YPSAC 5% liquid medium, sterilized and 0.10 grams of yeasts FT858 and Pedra-2 were inoculated and incubated at 30 °C for 10 h at 250 rpm. After the production, the cells were recovered by centrifugation, at 105 rpm, resulting in a 10 mg mg-1 concentration of moist dough. This dough was subsequently inoculated in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomass; Saccharomyces cerevisiae; Bioethanol.
Ano: 2022
URL: http://periodicosonline.uems.br/index.php/agrineo/article/view/6719

Uso de levaduras y fumarato para disminuir la metanogénesis en la fermentación de alfalfa.
Provedor de dados: 32
Autores: Báez Pérez, José Luis.
Se realizaron tres experimentos en biorreactores acoplados a trampas de gas para evaluar el efecto de Saccharomyces cerevisiae y fumarato de sodio en la fermentación in vitro de alfalfa. En el primero se evaluó la fermentación de alfalfa en medio de cultivo glucosa, celobiosa, almidón y fluido ruminal (GCAFR) usando trampas de gas con agua, solución salina ácida y solución de NaOH. Se midió producción de biogás, CH4, CO2, pH y bacterias totales (BT). El diseño experimental fue completamente al zar con arreglo factorial 2 x 3, y tres repeticiones por tratamiento. En el segundo experimento se evaluó el efecto de Yea sacc®, Procreatin 7® y Diamond V XP® en la fermentación in vitro de alfalfa. Se midió producción de CH4, ácidos grasos volátiles (AGV),...
Palavras-chave: Fermentación ruminal; Biogás; CH4; Saccharomyces cerevisiae; Fumarato de sodio; Rumen fermentation; Biogas; Sodium fumarate; Ganadería; Doctorado.
Ano: 2010
URL: http://hdl.handle.net/10521/85

Comportamiento productivo y niveles de ácido oleico en la canal de corderos suplementados con Saccharomyces cerevisiae.
Provedor de dados: 32
Autores: Gloria Trujillo, Adrián.
Los altos niveles de ácidos grasos saturados en la carne de rumiantes representan un  punto crítico en la alimentación humana por su relación con enfermedades cardiovasculares. La levadura Saccharomyces cerevisiae (Sc) es rica en ácido oleico y representa una alternativa para modificar el perfil de ácidos grasos de la carne, al incrementar la proporción de ácidos grasos insaturados. Por lo anterior, se realizó un experimento con corderos en crecimiento (20± 0.9 kg PV), utilizando una dieta a base de concentrado y tres niveles (tratamientos, T) de Sc (0, 3 y 5 g animal-1 d-1, T1, T2 y T3, respectivamente), con el objetivo de determinar el comportamiento animal, caracterizar la fermentación ruminal, los niveles plasmáticos de colesterol y los ácidos grasos...
Palavras-chave: Saccharomyces cerevisiae; Oleico; Corderos; Fermentación ruminal; Colesterol; Oleic; Lambs; Ruminal fermentation; Cholesterol; Ganadería; Maestría.
Ano: 2012
URL: http://hdl.handle.net/10521/773

Uso de levaduras y fumarato para disminuir la metanogénesis en la fermentación de alfalfa.
Provedor de dados: 32
Autores: Báez Pérez, José Luis.
Se realizaron tres experimentos en biorreactores acoplados a trampas de gas para evaluar el efecto de Saccharomyces cerevisiae y fumarato de sodio en la fermentación in vitro de alfalfa. En el primero se evaluó la fermentación de alfalfa en medio de cultivo glucosa, celobiosa, almidón y fluido ruminal (GCAFR) usando trampas de gas con agua, solución salina ácida y solución de NaOH. Se midió producción de biogás, CH4, CO2, pH y bacterias totales (BT). El diseño experimental fue completamente al zar con arreglo factorial 2 x 3, y tres repeticiones por tratamiento. En el segundo experimento se evaluó el efecto de Yea sacc®, Procreatin 7® y Diamond V XP® en la fermentación in vitro de alfalfa. Se midió producción de CH4, ácidos grasos volátiles (AGV),...
Palavras-chave: Fermentación ruminal; Biogás; CH4; Saccharomyces cerevisiae; Fumarato de sodio; Rumen fermentation; Biogas; Sodium fumarate; Ganadería; Doctorado.
Ano: 2010
URL: http://hdl.handle.net/10521/85

Comportamiento productivo y niveles de ácido oleico en la canal de corderos suplementados con Saccharomyces cerevisiae.
Provedor de dados: 32
Autores: Gloria Trujillo, Adrián.
Los altos niveles de ácidos grasos saturados en la carne de rumiantes representan un  punto crítico en la alimentación humana por su relación con enfermedades cardiovasculares. La levadura Saccharomyces cerevisiae (Sc) es rica en ácido oleico y representa una alternativa para modificar el perfil de ácidos grasos de la carne, al incrementar la proporción de ácidos grasos insaturados. Por lo anterior, se realizó un experimento con corderos en crecimiento (20± 0.9 kg PV), utilizando una dieta a base de concentrado y tres niveles (tratamientos, T) de Sc (0, 3 y 5 g animal-1 d-1, T1, T2 y T3, respectivamente), con el objetivo de determinar el comportamiento animal, caracterizar la fermentación ruminal, los niveles plasmáticos de colesterol y los ácidos grasos...
Palavras-chave: Saccharomyces cerevisiae; Oleico; Corderos; Fermentación ruminal; Colesterol; Oleic; Lambs; Ruminal fermentation; Cholesterol; Ganadería; Maestría.
Ano: 2012
URL: http://hdl.handle.net/10521/773

Production of ethanol from mesquite (Prosopis juliflora (SW) D.C.) pods mash by Zymomonas mobilis and Saccharomyces cerevisiae
Provedor de dados: 69
Autores: da Silva,Celiane Gomes Maia; Stamford,Tânia Lúcia Montenegro; de Andrade,Samara Alvachian Cardoso; de Souza,Evandro Leite; de Araújo,Janete Magali.
This study aimed to assess the use of mesquite pods hydrated mash as biomass for the growth of Saccharomyces cerevisiae UFEPEDA-1012 and Zymomonas mobilis UFEPEDA-205 and for ethanol production using a submerged fermentation. A 2³ factorial design was used to analyze the effects of the type of microorganism, time of fermentation and condition of cultivation on the ethanol production in mesquite pods mash (30 g 100 mL-1). From the obtained results the hydrated mesquite pods mash presented as a good substrate for the growth of S. cerevisiae and Z. mobilis in comparison to the standard media. The effect that most affected the ethanol production was the type of microorganism. The highest ethanol concentration (141.1 gL-1) was found when Z. mobilis was...
Tipo: Journal article
Palavras-chave: Ethanol; Mesquite; Saccharomyces cerevisiae; Zymomonas mobilis.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500012

Inhibitor tolerance and flocculation of a yeast strain suitable for second generation bioethanol production
Provedor de dados: 69
Autores: Westman,Johan O; Taherzadeh,Mohammad J; Franzén,Carl Johan.
Background: Robust second generation bioethanol processes require microorganisms able to ferment inhibitory lignocellullosic hydrolysates. In this study, the inhibitor tolerance and flocculation characteristics of Saccharomyces cerevisiae CCUG53310 were evaluated in comparison with S. cerevisiae CBS8066. Results: The flocculating strain CCUG53310 could rapidly ferment all hexoses in dilute acid spruce hydrolysate, while CBS8066 was strongly inhibited in this medium. In synthetic inhibitory media, CCUG53310 was more tolerant to carboxylic acids and furan aldehydes, but more sensitive than CBS8066 to phenolic compounds. Despite the higher tolerance, the increase in expression of the YAP1, ATR1 and FLR1 genes, known to confer resistance to...
Tipo: Journal article
Palavras-chave: Biofuel; Furfural; Lignocellulose; Phenolic inhibitors; Saccharomyces cerevisiae.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300005

Gene expression of specific enological traits in wine fermentation
Provedor de dados: 69
Autores: Martínez,Claudio; García,Verónica; González,David; Jara,Matías; Aguilera,Matías; Ganga,Maria Angélica.
Background: Wine fermentation is a dynamic process and yeast has a precise genic regulation system that allow them to coordinate the gene transcription levels. However, the fermentation stage at which there is a great variation in the genic expression associated to a particular enological trait is often unknown and there are no simple experimental approaches to define it. Results: To identify the most adequate stage in which to evaluate the expression of the genes associated to specific enological traits we identified three stages of fermentation using the industrial strain EC1118. These stages, called early (0-16.4 hrs), middle (16.4-88.7 hrs) and late (88.7 hrs onwards), were characterized according to changes in the speed of CO2 production. The greatest...
Tipo: Journal article
Palavras-chave: Enological traits; Gene expression; Saccharomyces cerevisiae; Wine fermentation; Wine yeast.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400013

RNA-binding proteins and their role in the regulation of gene expression in Trypanosoma cruzi and Saccharomyces cerevisiae
Provedor de dados: 74
Autores: Oliveira,Camila; Faoro,Helisson; Alves,Lysangela Ronalte; Goldenberg,Samuel.
Abstract RNA-binding proteins (RBPs) have important functions in the regulation of gene expression. RBPs play key roles in post-transcriptional processes in all eukaryotes, such as splicing regulation, mRNA transport and modulation of mRNA translation and decay. RBPs assemble into different mRNA-protein complexes, which form messenger ribonucleoprotein complexes (mRNPs). Gene expression regulation in trypanosomatids occurs mainly at the post-transcriptional level and RBPs play a key role in all processes. However, the functional characterization of RBPs in Trypanosoma cruzi has been impaired due to the lack of reliable reverse genetic manipulation tools. The comparison of RBPs from Saccharomyces cerevisiae and T. cruzi might allow inferring on the function...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNA-binding proteins; Trypanosoma cruzi; Saccharomyces cerevisiae; Gene expression regulation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100022

Characterization of biochemical compounds and antioxidant activity of “dedo-de-moça” chili pepper accessions
Provedor de dados: 75
Autores: Gomes,Gisely Paula; Constantino,Leonel Vinicius; Corte,Ligia Erpen-Dalla; Riger,Cristiano Jorge; Chaves,Douglas S de A; Gonçalves,Leandro SA.
ABSTRACT Capsicum baccatum is one of the main chili pepper species grown and consumed in South America. In Brazil, C. baccatum var. pendulum is widely cultivated, especially “dedo-de-moça” type. For most horticultural crops, including Capsicum species their diversity remains under-explored for traits related to fruit quality, since over the past half century breeding efforts have focused mainly on good agronomic performances. The investment in screening traits such as flavor, nutritional compounds and antioxidant content in traditional varieties is very important to support fruit quality breeding efforts. Thus, the objective of this study was accessing the variations into content of soluble solid, acidity, capsaicinoid, vitamin C, total phenolic compounds...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capsicum baccatum var. pendulum; Saccharomyces cerevisiae; Fruit quality; Plant breeding.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362019000400429

Uso de antibióticos e leveduras para controle da podridão-mole em couve-chinesa
Provedor de dados: 75
Autores: Mello,MRF; Silveira,EB; Viana,IO; Guerra,ML; Mariano,RLR.
A podridão-mole causada por Pectobacterium carotovorum subsp. carotovorum (Pcc) pode ocorrer em plantios de couve-chinesa (Brassica pekinensis) com até 67% de incidência. O trabalho visou avaliar a sensibilidade in vitro de Pcc a bactericidas, o efeito de Mycoshield® nas dosagens de 3,0 e 1,5 g L-1, e de leveduras a 10(8) cel/mL no controle da doença em casa de vegetação e em campo. As plantas foram pulverizadas com Mycoshield® (oxitetraciclina 20%) e leveduras (Rh1 e Rh2 (Rhodotorula spp.) e Sc1 (Saccharomyces cerevisae)) sete dias após o transplante, e inoculadas por picada com o isolado Pcc120, sete dias e 12 h após o tratamento, respectivamente. Em todos os experimentos foram avaliados os componentes epidemiológicos da doença. In vitro, 40 isolados de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brassica pekinensis; Pectobacterium carotovorum subsp. carotovorum; Saccharomyces cerevisiae; Rhodotorula sp.; Oxitetraciclina; Estreptomicina.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000100013

Improved Leavening Ability of a Wild Yeast, Saccharomyces cerevisiae AK46 2-deoxyglucose Resistant Mutant
Provedor de dados: 19
Autores: Mikumo, Dai; Takaya, Masahiro; Orikasa, Yoshitake; Ohwada, Takuji; 折笠, 善丈; 大和田, 琢二.
The wild yeast Saccharomyces cerevisiae AK46 was isolated from cherry fruits and commercialized as baker's yeast. We isolated a 2-deoxyglucose (2-DOG)-resistant mutant of AK46 (MCD4) and evaluated its leavening ability in bread dough. Our findings show that the maltose utilization of MCD4 was significantly enhanced in the presence of glucose and α-glucosidase activity was increased by approx. 1.6-fold compared to that of AK46, indicating release from catabolite repression. In addition, baking performance results using the sponge dough and no-time dough methods showed that the CO2 production rate of MCD4 was increased by approx. 1.6-fold (sponge and dough method) and the specific volume of bread was increased by 6.3% and 7.8%, respectively. These results...
Palavras-chave: Bread dough; Saccharomyces cerevisiae; 2-deoxyglucose; AK46; Leavening.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4368

Discrimination of Saccharomyces cerevisiae and Saccharomyces paradoxus strains by the SUC2 gene sequences
Provedor de dados: 19
Autores: Oda, Yuji; Mikumo, Dai; Leo, Fiame; Urashima, Tadasu; 小田, 有二; 浦島, 匡.
http://www.jstage.jst.go.jp/article/jgam/56/4/56_355/_article/-char/ja/
Palavras-chave: Saccharomyces cerevisiae; Saccharomyces paradoxus; SUC2 gene.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3058

産学官連携による製パン用「とかち野酵母」の開発
Provedor de dados: 19
Autores: 小田, 有二; 山内, 宏昭; 田村, 雅彦; Oda, Yuji; Yamauchi, Hiroaki; Tamura, Masahiko.
平成23年度日本食品科学工学会技術賞
Palavras-chave: パン酵母; Saccharomyces cerevisiae; 製パン.
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3572

Baker's yeast as a potential substitute for live algae in aquaculture diets: &lt;i&gt;Artemia&lt;/i&gt; as a case study
Provedor de dados: 27
Autores: Coutteau, P.; Lavens, P.; Sorgeloos, P.
In this study baker's yeast was evaluated as a substitute for live &lt;i&gt;Dunaliella tertiolecta&lt;/i&gt; algae in the culture of the brine shrimp &lt;i&gt;Artemia&lt;/i&gt; . Consumption of fresh baker's yeast resulted in poor growth and survival of brine shrimp. However, the nutritional value of the yeast significantly improved after complete removal of the yeast cell wall by enzymatic treatment. Baker's yeast was also made digestible for &lt;i&gt;Artemia&lt;/i&gt; by simple chemical treatment which did not reduce rigidity of the yeast cell. The external mannoprotein layer of the yeast cell wall is probably the major barrier to digestion by &lt;i&gt;Artemia&lt;/i&gt; . Chemically treated baker's yeast offers promising possibilities as a substitute for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diets; Yeasts; Artemia; Saccharomyces cerevisiae.
Ano: 1990
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3064

Qualidade dos ovos de poedeiras comerciais alimentadas com levedura seca de cana-de-açúcar
Provedor de dados: 86
Autores: Maia,George André Rodrigues; Fonseca,José Brandão; Soares,Rita da Trindade Ribeiro Nobre; Silva,Martinho de Almeida e; Souza,Cláudio Luiz Melo de.
Este trabalho objetivou avaliar o efeito da adição de levedura seca (Saccharomyces cerevisiae) à dieta de poedeiras na qualidade dos ovos. Foram utilizadas 120 poedeiras comerciais com 33 semanas de idade, distribuídas em um delineamento estatístico de blocos ao acaso, com cinco tratamentos (0, 7, 14, 21 e 28% de levedura), quatro repetições e seis aves por unidade experimental. Rações isoprotéicas (18% de proteína bruta), isoenergéticas (2.800 kcal de energia metabolizável/kg), isocálcicas (3,8% Ca) e isofosfóricas (0,38% P disponível) foram formuladas à base de milho e farelo de soja. Os níveis de levedura seca não alteraram o peso do ovo (64,35± 0,85 g), a altura do albúmen (7,95±0,22 mm), a unidade Haugh (87,42±1,31), os sólidos totais da gema...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Alimento; Nutrição animal; Ave.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2002000900013

Controle alternativo de podridões pós‑colheita de framboesas
Provedor de dados: 86
Autores: Antoniolli,Lucimara Rogéria; Silva,Gildo Almeida da; Alves,Silvio André Meirelles; Moro,Laís.
O objetivo deste trabalho foi avaliar o efeito de tratamentos pré-colheita sobre a ocorrência de podridões pós-colheita e sobre os atributos de qualidade de framboesas (Rubus idaeus L.) 'Heritage'. As frutas foram pulverizadas com um dos seguintes tratamentos: água destilada (controle), 6 g L-1 de quitosana, 100 mg L-1 de dióxido de cloro, Bacillus amyloliquefaciens, Curtobacterium pusillum ou Saccharomyces cerevisiae. Foram realizadas colheitas aos 3, 7 e 14 dias após a aplicação dos tratamentos. Após cada uma das colheitas, realizadas no estádio de maturação comercial (coloração rosa), as frutas foram inoculadas individualmente com suspensão de conídios (2x10(5) conídios mL-1) de Botrytis cinerea ou Rhizopus stolonifer. As frutas foram mantidas a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus amyloliquefaciens; Curtobacterium pusillum; Rubus idaeus L; Saccharomyces cerevisiae; Controle biológico; Pequenas frutas; Quitosana.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000900002

Crescimento, digestibilidade e resistência à infecção por patógeno em tilápia-do-nilo alimentada com probióticos
Provedor de dados: 86
Autores: Cornélio,Fernando Henrique Gomes; Cargnin-Ferreira,Eduardo; Borba,Maude Regina de; Mouriño,José Luiz Pedreira; Fernandes,Vitor Augusto Giatti; Fracalossi,Débora Machado.
O objetivo deste trabalho foi avaliar o efeito da suplementação da dieta com os probióticos Lactobacillus plantarum e Saccharomyces cerevisiae, no desempenho zootécnico, digestibilidade e na resistência à infecção por patógeno, em alevinos de tilápia-do-nilo. Foram realizados três ensaios. No primeiro, durante 55 dias, seis grupos de 30 alevinos (2,4±0,5 g) receberam suplementação com probióticos, e outros três grupos não receberam suplementação. No final desse período, no segundo ensaio, os peixes foram desafiados com Aeromonas hydrophila, e a sobrevivência foi avaliada por 96 horas. No terceiro ensaio, com oito peixes por tanque (230,0±10,0 g), avaliou-se a digestibilidade da dieta após a suplementação com os probióticos. A suplementação probiótica...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aeromonas hydrophila; Lactobacillus plantarum; Oreochromis niloticus; Saccharomyces cerevisiae; Desempenho produtivo; Nutrição.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000800011

Epididymal Sperm Characteristics, Testicular Morphometric Traits and Growth Parameters of Rabbit Bucks Fed Dietary Saccharomyces cerevisiae and/or Zinc Oxide
Provedor de dados: 89
Autores: Emmanuel,DC; Amaka,AE; Okezie,ES; Sunday,UP; Ethelbert,OC.
ABSTRACT The objectives of this twelve-week feeding trial were to determine the effects of dietary Saccharomyces cerevisiae and/or zinc oxide on epididymal sperm characteristics, testicular morphometric traits, and growth parameters of bucks. 16 (New Zealand White) bucks 16-wk-old, weighing 2.8kg were randomly allotted to one of 4 treatment groups. Each treatment was replicated four times, with 1 buck per replicate, in a completely randomized design (CRD). Each treatment group was randomly assigned to one of the four commercial experimental diets designated thus: TA = control diet with no additives, TB = 0.12g/kg Saccharomyces cerevisiae, TC = 150mg/kg zinc oxide and TD = 0.12g/kg Saccharomyces cerevisiae +150 mg/kg zinc oxide. Although treatment had no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nutrition; Bucks; Semen; Saccharomyces cerevisiae; Zinc oxide.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100311

Effect of an Enzymatic Blend and Yeast on the Performance, Carcass Yield and Histomorphometry of the Small Intestine in Broilers from 21 to 42 Days of Age
Provedor de dados: 89
Autores: Sousa,RF; Dourado,LRB; Lopes,JB; Fernandes,ML; Kato,RK; Nascimento,DCN; Sakomura,NK; Lima,SBP; Ferreira,GJBC.
ABSTRACT The present study was conducted to evaluate the performance, carcass yield, and histomorphometry of the small intestine of broilers fed yeast sugarcane supplemented with enzymatic blend from 22 to 42 days of age. Seven hundred broiler chicks were distributed in a completely randomized design in a factorial design (2x3+1), two levels of the enzyme blend (0 and 200 g/ton), three protein levels (0%, 6% and 12%) and a control diet. The level of 6% yeast showed higher feed intake and weight gain after 33 days. From 22 to 42 days there was no significant effect (p&lt;0.05) to the use of yeast in animal performance, carcass yield and cuts. At 42 days an interaction between the factors for width and crypt depth in the duodenum and jejunum, respectively,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cane sugar; Microscopy; Metabolism; Saccharomyces cerevisiae; Xylanase.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200300

Saccharomyces Cerevisiae cell wall dietary supplementation on the performance and intestinal mucosa development and integrity of broiler chickens vaccinated against coccidiosis
Provedor de dados: 89
Autores: Luquetti,BC; Furlan,RL; Alarcon,MFF; Macari,M.
This study was carried out to verify if Saccharomyces cerevisiae cell wall (SCCW) dietary supplementation (0.2%) was capable of protecting the intestinal mucosa of broiler chickens vaccinated against coccidiosis. Body weight gain, feed intake, feed conversion and intestinal mucosa morphometric parameters and epithelial loss were evaluated. In the experiment,400 day-old male chicks were distributed according to a completely randomized design in a 2x2 factorial arrangement. The following treatments were applied: T1 - no vaccination/ no SCCW supplementation; T2 - no vaccination/SCCW supplementation; T3 - vaccination/no SCCW supplementation; and T4 - vaccination/SCCW supplementation to four replicates of 25 birds each. Birds were vaccinated on the first day of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coccidiosis; Vaccine; Broiler; Intestinal mucosa; Morphometrics; Saccharomyces cerevisiae.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2012000200002

Cinética de secagem do resíduo de abacaxi enriquecido
Provedor de dados: 91
Autores: Alexandre,Hofsky V.; Silva,Flávio L. H. da; Gomes,Josivanda P.; Silva,Osvaldo S. da; Carvalho,João P. D.; Lima,Ezenildo E. de.
O Brasil é um dos maiores produtores de abacaxi do mundo e o maior da América do Sul. O fruto é a parte comercializável da planta enquanto o restante é formado por caule, folha, casca, coroa e talos, considerado resíduo agrícola e não tem sido devidamente aproveitado, resultando em perdas econômicas. Caracterizar e avaliar a cinética de secagem do resíduo (casca) de abacaxi (Ananas comosus L.) enriquecido utilizando a levedura Saccharomyces cerevisiae em leito estático, em camada fina de secagem, utilizando-se de planejamento experimental fatorial, foi o objetivo deste estudo. A temperatura (40 a 60 ºC) e a velocidade do ar de secagem (0,8 a 1,3 m s-1) são as variáveis do processo. O resíduo enriquecido e caracterizado apresentou alto teor de matéria seca...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ananas comosus L.; Saccharomyces cerevisiae; Secagem.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662013000600010

Cinética de produção de levedura seca em leito de jorro
Provedor de dados: 91
Autores: Rocha,Ana P. T.; Alsina,Odelsia L. S.; Silva,Vimário S.; Silva,Flávio L. H. da.
Estudou-se neste trabalho, a cinética de produção durante a secagem em um secador de leito de jorro convencional com inertes, trabalhando-se com alimentação intermitente de levedura, com umidade inicial de 70% em base úmida. Fez-se um planejamento fatorial 22 mais configuração estrela com 3 experimentos no ponto central, totalizando 11 experimentos, objetivando-se o estudo da influência da concentração de levedura e velocidade do ar na entrada sobre a cinética de produção de levedura seca. A cinética se mostrou crescente e praticamente linear em todos os experimentos. Esta análise permitiu a identificação das condições ideais de operação do secador com relação à velocidade de operação e de quantidade de levedura a ser alimentada para obtenção de um produto...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Secagem; Armazenamento; Saccharomyces cerevisiae.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662008000100012

Feed efficiency in Mangalarga Marchador foals fed diet supplemented with probiotics or phytase
Provedor de dados: 96
Autores: Moura,Raquel Silva de; Saliba,Eloísa de Oliveira Simões; Almeida,Fernando Queiroz de; Lana,Ângela Maria Quintão; Silva,Vinícius Pimentel; Rezende,Adalgiza Souza Carneiro de.
The objective was to assess the effect of probiotic or phytase supplementation on dry matter intake (DMI), average daily gain (ADG) and feed conversion (FC) in 24 Mangalarga Marchador foals (16 males, 8 females), with 241.6 ± 23.2 days of age, and 216.7 ± 27.8 kg of body weight (BW). The study lasted 28 days, the first 23 days for diet and handling adaptation, and the last five days for feces collection. The basal diet consisted of Cynodon pasture and pelleted concentrate (1.2 kg/100 kg BW), as well as water and mineral salt ad libitum. Treatments groups were assigned as follows: basal diet (control); basal diet + 5 g/day of live yeast culture (10(9) CFU/g of S. cerevisiae); basal diet + 2 g/day of live bacteria (10(8) CFU/g of B. bifidum, E. faecium, L....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymes; Horses; Lactobacillus; LIPE®; Pasture intake; Saccharomyces cerevisiae.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009000600011

Levels of yeast and its by-products on pacu juveniles feeding
Provedor de dados: 96
Autores: Watanabe,André Luiz; Viegas,Elisabete Maria Macedo; Gonçalves,Lígia Uribe.
This study was conducted to evaluate the inclusion of two levels (2.5 e 5.0%) of dried yeast (Saccharomyces cerevisiae) and its by-products, disrupted yeast cells and yeast cell wall in diets for juveniles of pacu (Piaractus mesopotamicus). Production performance, body and plasmatic composition indexes were evaluated. Seven isoproteic (26% digestible protein) and isoenergetic (3.100 kcal digestible energy) diets were formulated containing increased levels of each ingredient. The diets were supplied for 86 days, "ad libitum". Yeast and by-products increase feed efficiency and protein use, when compared to the control diet. Carcass composition and plasmatic (glucose, cortisol, uric acid, urea and plasmatic protein) levels are not affected by the test...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Piaractus mesopotamicus; Pre-biotic; Production performance; Saccharomyces cerevisiae; Yeast by-products.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000300001

Nutritive value of diets containing inactive dry yeast for lactating Saanen goats
Provedor de dados: 96
Autores: Gomes,Ludmila Couto; Alcalde,Claudete Regina; Lima,Larissa Ribas de; Lima,Luciano Soares de; Souza,Rodrigo de; Possamai,Ana Paula Silva.
Twenty-four Saanen goats, 15 multiparous and nine primiparous, were distributed in a completely randomised design in a factorial arrangement (3 diets × 2 parities). The treatments were soybean meal (SB), soybean + dry yeast (SBDY), or dry yeast (DY) as a protein source in the diet, and ground corn, mineral supplement, and corn silage (400 g/kg). The study was conducted to evaluate the nutritive value of diets containing inactive dry yeast as a protein source postpartum, postpeak, and during late lactation in dairy goats. To estimate the faecal excretion of indigestible material, neutral detergent fibre was used as an internal marker. The diets did not affect dry matter intake (DMI). However, DMI and nutrient intake were higher for multiparous goats...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Digestibility; Intake; Parturition order; Saccharomyces cerevisiae; Urea nitrogen.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000100006

Desempenho de frangos de corte de 1 a 21 dias de idade alimentados com rações contendo extrato de leveduras e prebiótico e criados em diferentes temperaturas
Provedor de dados: 96
Autores: Silva,Vanessa Karla; Silva,Janaina Della Torre da; Gravena,Rodrigo Antonio; Marques,Rafael Henrique; Hada,Fabricio Hirota; Moraes,Vera Maria Barbosa de.
Avaliou-se o desempenho de frangos criados em diferentes temperaturas e alimentados na fase pré-inicial com ração contendo ou não extrato de leveduras e/ou prebiótico. Foram utilizados 1.440 pintos Cobb 500®, machos de 1 dia de idade, criados em diferentes câmaras climáticas. As rações experimentais foram oferecidas somente na fase pré-inicial (1 a 7 dias), de modo que, a partir do oitavo dia, todas as aves receberam a mesma ração. Adotou-se o delineamento experimental inteiramente casualizado, em arranjo fatorial 3 × 2 × 2, composto de 3 temperaturas de criação (alta 34 ± 1ºC; controle 32 ± 2ºC; e baixa 27 ± 2ºC), 2 níveis de extrato de levedura (com ou sem) e 2 níveis de prebiótico (com ou sem). As temperaturas ambiente alta e baixa prejudicaram o...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Estresse térmico; Mananoligossacarídeos; Ração pré-inicial; Saccharomyces cerevisiae.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009000400015

Uso de produto à base de enzima e levedura na dieta de bovinos: digestibilidade dos nutrientes e desempenho em confinamento
Provedor de dados: 96
Autores: Queiroz,Ralf Cezar de; Bergamaschine,Antônio Fernando; Bastos,João Francisco Pereira; Santos,Pedro César dos; Lemos,Guilherme Corbucci.
Objetivou-se, com este trabalho, avaliar os efeitos da adição de diferentes níveis de enzima mais levedura na dieta de bovinos sobre a digestibilidade aparente dos nutrientes e o desempenho em confinamento, utilizando-se 18 animais machos, mestiços, de origem leiteira, com peso vivo (PV) médio de 190±10 kg e 12 meses de idade, e 27 animais da raça Guzerá com PV médio de 325 kg e idade média de 27 meses. Os níveis de enzima mais levedura foram zero (controle), 5 g de enzima (E) mais 5 g de levedura (L) e 10 g de E mais 5 g de L por animal por dia. Todos os animais receberam a mesma dieta, constituída de 65% de silagem (50% de milho:50% de sorgo) e 35% de concentrado composto de milho (24,6%), farelo de algodão (7,4%), uréia (1,2%), sal mineralizado (0,54%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aditivo; Ruminante; Saccharomyces cerevisiae; Xilanase.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982004000600022

Sugarcane yeast inclusion for broilers at post-hatch
Provedor de dados: 96
Autores: Lopes,Cláudia da Costa; Rabello,Carlos Bôa-Viagem; Silva,Jaqueline de Cássia Ramos da; Arruda,Emmanuele Maria Florêncio de; Ludke,Maria do Carmo Mohaupt Marques; Dutra Junior,Wilson Moreira.
ABSTRACT A total of 450 one-day-old male broiler chicks were used to evaluate the effect of the sugarcane yeast on performance, body composition, and development of the intestinal mucosa. The experiment was carried out in a completely randomized design with five treatments and six replicates of 15 birds. Sugarcane yeast was included in the experimental diets at the levels of 0, 12.5, 25.0, 37.5, and 50.0 g kg−1. Body weight, weight gain, feed intake, and feed conversion were determined. At the end of the experiment, four broiler chicks were slaughtered per experimental unit: two were used for evaluation of moisture content, crude protein, fat, and body ash and the other two were used in the collection of small-intestine segments for evaluation of villus...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Body composition; Broiler; Intestinal morphology; Performance; Saccharomyces cerevisiae.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017001200924

Performance of lactating goats fed diets containing inactive dry yeast
Provedor de dados: 96
Autores: Gomes,Ludmila Couto; Alcalde,Claudete Regina; Macedo,Francisco de Assis Fonseca de; Santos,Geraldo Tadeu dos; Valloto,Altair Antônio; Lima,Luciano Soares de; Molina,Bruna Susan de Labio.
Twenty-four Saanen goats (15 multiparous and 9 primiparous) from 21 days prepartum to 200 days in lactation were used to evaluate milk yield and composition. Animals were randomly distributed in a 3 × 2 factorial arrangement (3 diets × 2 parity orders) and fed a 40:60 forage:concentrate diet composed of soybean meal, soybean meal + dry yeast or dry yeast as protein source, plus ground corn, mineral supplement and corn silage. The protein source did not influence the body weight of pre and postpartum goats. Prepartum, postpartum and postpeak dry matter intake (DMI) were not altered by diets. However, goats fed the diet containing dry yeast as protein source had lower DMI in late lactation. Milk yield and feed efficiency were not affected by diets. Milk...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feed additive; Lactation; Parity; Saccharomyces cerevisiae.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000016

Quantitative characteristics of carcass and meat quality of ¾ Boer + ¼ Saanen and Saanen goat kids fed diets with dry yeast
Provedor de dados: 96
Autores: Freitas,Hanna Sakamoto; Alcalde,Claudete Regina; Lima,Luciano Soares de; Macedo,Francisco de Assis Fonseca de; Macedo,Vicente de Paulo; Molina,Bruna Susan de Labio.
Twenty-seven carcasses of non-castrated confined goat kids, (18 ¾ Boer + ¼ Saanen and nine Saanen) with slaughtered weight of 30.1 ± 0.27 kg were used to evaluate quantitative characteristics of the carcass and qualitative characteristics of the loin and shoulder. The animals were distributed in a completely randomized design in a 2 × 3 factorial arrangement (breed group × diet). The diets consisted of inclusion of dry yeast, as follows: soybean meal, soybean meal + dried yeast or dried yeast. The other ingredients of the diets were oat hay (30% DM), ground corn and mineral mixture. Hot carcass weight, cold carcass weight, weight loss by cooling, true carcass yield and carcass compactness index were not influenced by the inclusion of dry yeast nor by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Byproduct of sugar cane; Genetic group; Goat; Meat quality; Saccharomyces cerevisiae.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000300023

Sugar cane dry yeast in feeding for growing and finishing goat kids
Provedor de dados: 96
Autores: Lima,Luciano Soares de; Alcalde,Claudete Regina; Macedo,Francisco de Assis Fonseca de; Lima,Larissa Ribas de; Martins,Elias Nunes; Coutinho,Carolina Cesarino.
It was used 27 goat kids (18 ¾ Boer + ¼ Saanen and nine Saanen; 18.0 ± 1.60 kg) distributed in a completely randomized design with factorial arrangement to evaluate productive performance and the total digestibility of dry matter and nutrients in the rations. Diets were composed of soybean meal, soybean meal + dry yeast or dry yeast as protein source, oat hay, ground corn and vitamin-mineral mixture with mean composition of 2.50 Mcal of ME/kg DM and 17.0% of crude protein on average. Digestibility was determined using indigestible neutral detergent fiber as marker. Intakes of dry matter, organic matter, crude protein and total carbohydrates were not altered by rations. However, inclusion of dry yeast in the rations reduced ingestions of ether extract,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Digestibility; Goats; Intake; Performance; Saccharomyces cerevisiae.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000100024

Digestibilidade parcial e total de rações com a inclusão de ionóforo ou probiótico para bubalinos e bovinos
Provedor de dados: 96
Autores: Zeoula,Lucia Maria; Beleze,Juliano Ricardo Fontanini; Geron,Luiz Juliano Valério; Maeda,Emilyn Midori; Prado,Ivanor Nunes do; Paula,Meiby Carneiro de.
Avaliaram-se os efeitos do ionóforo (monensina sódica) e do probiótico (Saccharomyces cerevisiae + selênio + cromo) em rações com 50:50% de volumoso:concentrado sobre os coeficientes de digestibilidade total (CDT) e parcial dos nutrientes. Foram utilizados três búfalos da raça Murrah e três bovinos da raça holandesa, com peso médio de 477 ± 47 e 518 ± 56 kg, respectivamente, portadores de cânula ruminal e duodenal, distribuídos em delineamento experimental com dois quadrados latinos 3 &lt;FONT FACE=Symbol&gt;´&lt;/FONT&gt; 3 e arranjo fatorial 3 &lt;FONT FACE=Symbol&gt;´&lt;/FONT&gt; 2. O indicador dos fluxos fecal e duodenal foi a cinza insolúvel em ácido. Não houve interação dieta &lt;FONT FACE=Symbol&gt;´&lt;/FONT&gt; espécie animal para os CTD dos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aditivo; Monensina sódica; Bovídeos; Saccharomyces cerevisiae.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982008000300023

Animal performance and nutrient digestibility of feedlot steers fed a diet supplemented with a mixture of direct-fed microbials and digestive enzymes
Provedor de dados: 96
Autores: Encinas,Christian Michael Alvarez; Villalobos,Guillermo Villalobos; Viveros,Joel Domínguez; Flores,Gabriela Corral; Almora,Enrique Alvarez; Rangel,Francisco Castillo.
ABSTRACT The objective of this study was to evaluate the effect of a mixture of direct-fed microbials (DFM), yeast, and digestive enzymes on animal performance in feedlot cattle, as well as digestibility when finishing feedlot cattle. Thirty crossbreed (Charolais × Beefmaster) steers averaging 15 months old and 321.83±3.73 kg of initial body weight were used. Animals were randomly assigned to one of two treatment groups: control (basal diet) and a mixture of DFM, yeast, and digestive enzymes (basal diet + 30 g of the mixture). Each group contained fifteen experimental units. Animals were fed individually twice a day, and dry matter intake (DMI) was recorded daily. Body weights were recorded initially and subsequently at 28-day intervals for a total of 140...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymes; Finishing; Probiotics; Saccharomyces cerevisiae; Yeast.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100615

Performance of dairy goats fed diets with dry yeast from sugar cane as protein source
Provedor de dados: 96
Autores: Lima,Luciano Soares de; Alcalde,Claudete Regina; Freitas,Hanna Sakamoto; Molina,Bruna Susan de Labio; Macedo,Francisco de Assis Fonseca de; Horst,José Augusto.
The effects of inactive dry yeast (Saccharomyces cerevisiae) from sugar cane were studied in 18 primiparus Saanen dairy goats (51.07±1.43) on dry matter intake and digestibility, milk production and quality. Animals were distributed in a completely randomized design during 90 days (from day 60 of milking). Diets were composed of soybean meal; soybean meal + dry yeast; or dry yeast, as protein sources, and ground corn, mineral supplement and corn silage (40%). Animals fed the dry yeast diet showed lower intake of dry matter (DM), organic matter (OM), crude protein, ether extract and neutral detergent fiber. Diets did not influence milk yield; however the milk production efficiency (kg of milk produced/kg of crude protein ingested) was better in goats fed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Digestibility; Goats; Milk quality; Saccharomyces cerevisiae.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000100033

The clarification of sugarcane juice and the use of CA-11 yeast produces better quality cachaça
Provedor de dados: 124
Autores: Mutton,Márcia Justino Rossini; Garcia,Graciany; Teixeira,Vitor; Silva,Aline Ferreira; Costa,Gustavo Henrique Gravatim; Ferreira,Osania Emerenciano.
ABSTRACT Cachaça is the second most consumed alcoholic beverage in Brazil, obtained by distilling wine from fermented sugarcane must, and has an alcohol content of 38 to 48% (v/v) at 20 ºC. The quality of the drink is affected by the raw material used, the treatment of the juice, the type of yeast and the distillation process. The aim of this study was to evaluate the performance of two types of yeast (selected and pressed) and the prior physical and chemical treatment of the sugarcane juice on the quality of the cachaça. The experimental design was completely randomised with 9 replications. The primary treatment was represented by the must (obtained from clarified and non-clarified juice) and the secondary treatment by the types of yeast (CA-11 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quality beverage; Liming; Selected yeast; Saccharomyces cerevisiae; Fermentation process.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000400408

Amino acid composition, and determination and prediction of the protein digestibility of different sugarcane yeasts in broilers
Provedor de dados: 124
Autores: Barbosa,Emanuela Nataly Ribeiro; Rabello,Carlos Bôa-Viagem; Lopes,Claudia Costa; Silva,Edney Pereira; Freitas,Ednardo Rodrigues.
ABSTRACT The aim of this study was to determine the amino acid composition and digestible protein values of different sugarcane yeasts, as well as to generate prediction equations for the apparent digestibility coefficient of dry matter (ADCDM) and crude protein (ADCCP), based on the chemical composition of the yeast and the age of the broilers. Four ileal digestion trials were carried out with 690 broilers, divided into the pre-starter, starter, growth and final phases. The birds were housed in metabolic cages and distributed in a completely randomised design, consisting of five treatments and six replications of eight, six, five and four birds respectively in each experimental phase. The treatments consisted of one reference feed and four test feeds,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amino acids; Ileal digestibility; Digestibile protein; Saccharomyces cerevisiae.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902018000200334

Interference in quality of strawberry caused by the use of biological agents for disease control
Provedor de dados: 22
Autores: Heling, A. L.; Favorito, P. A.; Kuhn, O. J.
The strawberry is a fruit of great market acceptance and it is much appreciated throughout the world, besides being very important for small farmers, generating employment and income. Nevertheless, this crop is affected by various diseases that require control, so that producers eventually use chemical fungicides, which, besides contaminating soil and water, eventually contaminate the fruit. In an attempt to reduce the amount of pesticides used in the strawberry crop, a lot of research on biological control of diseases has been done. However, the biological control of diseases in strawberries should not interfere negatively in fruit quality. Thus, this study aimed to evaluate the acidity, pH and total soluble solids (ºBrix) of strawberries treated with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus cereus; Controle biológico; Saccharomyces boulardii; Saccharomyces cerevisiae.
Ano: 2013
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/7869

Biological control of Mycosphaerella fragariae in strawberry culture
Provedor de dados: 22
Autores: Heling, Anderson Luis; Kuhn, Odair José; Stangarlin, José Renato.
The Mycosphaerella spot is one of the main foliar diseases of strawberry, degrating great leaf regions and reducing the photosynthetic area. Its control is mainly by the use of chemical fungicides, but, due the increasing demand for food free of pesticide, alternative control methods have been researched, such as biological control. This work aimed to evaluate the effect on strawberry plants, treated with the biological control agents Bacillus cereus, Saccharomyces boulardii and Saccharomyces cerevisiae, in the severity of Mycosphaerella fragariae, productivity and in the activity of β-1.3 glucanases, peroxidases and chitinases enzymes. It was verified that S. cerevisiae and B. cereus treatments were similar to fungicide for disease control. However, even...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Controle alternativo; Bacillus cereus; Saccharomyces boulardii; Saccharomyces cerevisiae.
Ano: 2015
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/9544

Comportamiento productivo y cambios morfológicos en vellosidades intestinales del pollo de engorda a 21 días de edad con el uso de paredes celulares del Saccharomyces cerevisiae
Provedor de dados: 108
Autores: Arce Menocal,José; Ávila González,Ernesto; López Coello,Carlos.
Se desarrolló un trabajo de investigación con el objetivo de evaluar paredes celulares del Saccharomyces cerevisiae (PcSc) a 500 g/t y avilamicina como antibiótico promotor de crecimiento (APC) a 10 ppm, en dietas sorgo + soya para pollo de engorda, sobre las variables productivas a los 21 días de edad, así como la longitud, ancho, número y área de las vellosidades intestinales a los diez y 21 días. Se utilizaron 200 pollitos de un día de edad, alojados en baterías y distribuidos mediante un diseño completamente al azar, con arreglo factorial 2 x 2 con cinco repeticiones de diez aves cada uno; los factores fueron: presencia y ausencia de PcSc y APC para las variables productivas. Los datos de las vellosidades intestinales se analizaron mediante un diseño...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paredes Celulares; Saccharomyces cerevisiae; Parámetros Productivos; Vellosidades Intestinales; Pollo de Engorda.
Ano: 2008
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0301-50922008000200011

Killer toxin of Saccharomyces cerevisiae Y500-4L active against Fleischmann and Itaiquara commercial brands of yeast
Provedor de dados: 59
Autores: Soares,Giselle A.M.; Sato,Hélia H.
The strain Saccharomyces cerevisiae Y500-4L, previously selected from the must of alcohol producing plants and showing high fermentative and killer capacities, was characterized according to the interactions between the yeasts and examined for curing and detection of dsRNA plasmids, which code for the killer character. The killer yeast S. cerevisiae Y500-4L showed considerable killer activity against the Fleischmann and Itaiquara commercial brands of yeast and also against the standard killer yeasts K2 (S. diastaticus NCYC 713), K4 (Candida glabrata NCYC 388) and K11 (Torulopsis glabrata ATCC 15126). However S. cerevisiae Y500-4L showed sensitivity to the killer toxin produced by the standard killer yeasts K8 (Hansenula anomala NCYC 435), K9 (Hansenula...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Killer yeast; Saccharomyces cerevisiae; Killer toxin.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000300012

A vector carrying the GFP gene (Green fluorescent protein) as a yeast marker for fermentation processes
Provedor de dados: 63
Autores: Gomes,Luiz Humberto; Duarte,Keila Maria Roncato; Andrino,Felipe Gabriel; Giacomelli,Ana Maria Brancalion; Tavares,Flavio Cesar Almeida.
Contaminant yeasts spoil pure culture fermentations and cause great losses in quality and product yields. They can be detected by a variety of methods although none being so efficient for early detection of contaminant yeast cells that appear at low frequency. Pure cultures bearing genetic markers can ease the direct identification of cells and colonies among contaminants. Fast and easy detection are desired and morphological markers would even help the direct visualization of marked pure cultures among contaminants. The GFP gene for green fluorescent protein of Aquorea victoria, proved to be a very efficient marker to visualize transformed cells in mixed populations and tissues. To test this marker in the study of contaminated yeast fermentations, the GFP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; GFP (green fluorescent protein); Fermentative processes.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000400018

EFEITOS DO TRATAMENTO COMBINADO DE IRRADIAÇÃO GAMA E REFRIGERAÇÃO NO CRESCIMENTO DE LEVEDURA (Saccharomyces cerevisiae Hansen) EM SUCO DE LARANJA
Provedor de dados: 63
Autores: DOMARCO,R.E; SPOTO,M.H.F; WALDER,J.M.M; BLUMER,L; MATRAIA,C.
O efeito da radiação gama e baixas temperaturas foi estudado no crescimento de Saccharomyces cerevisiae em suco de laranja. O suco concentrado de laranja (65°Brix) foi diluído a 10,5°Brix e inoculado com levedura comercial. As amostras foram irradiadas com doses de 1,0, 1,5, 2,0, 2,5, 5,0 e 7,5 kGy (Cobalto 60), a uma taxa de dose de 2,83 kGy/h. Todas as amostras foram armazenadas a 0°, 5° e 25°C, por 1, 7, 14, 21, 30, 60 e 90 dias. O tratamento combinado de 1 kGy e refrigeração a 0°C foi efetivo para a conservação do suco de laranja por 90 dias de armazenamento. A dose de 2,5 kGy foi suficiente para conservar o suco de laranja por 90 dias de armazenamento a 5°C. A 25°C, somente as doses mais altas (5 e 7,5 kGy) foram efetivas no controle do crescimento da...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Irradiação; Suco de laranja; Saccharomyces cerevisiae.
Ano: 1996
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161996000100003

ACÚMULO DE CÁDMIO POR Saccharomyces cerevisiae EM CALDO DE CANA-DE-AÇÚCAR CONTAMINADO COM ACETATO DE CÁDMIO
Provedor de dados: 63
Autores: Mariano-da-Silva,Samuel; Prado-Filho,Luíz Gonzaga do.
O presente trabalho visou estudar o acúmulo de cádmio (Cd) por Saccharomyces cerevisiae, fermentando mosto de caldo de cana-de-açúcar com contaminações controladas, em níveis sub-tóxicos do citado metal. As condições de fermentação foram similares às reinantes na produção industrial de etanol. O mosto, não esterelizado, continha 12% de açúcares redutores totais (ART) e pH 4,5. Para a contaminação controlada empregou-se acetato de cádmio em quatro níveis de contaminações (0,5; 1,0; 2,0 e 5,0 mg Cd kg-1 mosto). A inoculação do mosto foi executada com fermento de panificação (10% p/p). Após a fermentação (4 horas) foram determinados, porcentagem de fermento no vinho centrifugado e teor alcoólico do mesmo. Na levedura separada foram determinados peso úmido,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Caldo de cana; Metal pesado; Cádmio.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161999000200021

Diet digestibility and performance of dairy cows supplemented with live yeast
Provedor de dados: 63
Autores: Bitencourt,Luciene Lignani; Silva,José Ricardo Martins; Oliveira,Bruno Menezes Lopes de; Dias Júnior,Gilson Sebastião; Lopes,Fernanda; Siécola Júnior,Sancho; Zacaroni,Ozana de Fátima; Pereira,Marcos Neves.
Dietary yeast supplementation may improve the digestive efficiency of ruminants, but responses depend on the yeast strain and the diet composition. Corn silage and citrus pulp are usual carbohydrate sources for dairy cows in southeast Brazil. This study evaluated the supplementation of dairy cows fedding on corn silage-citrus pulp-based diets with Saccharomyces cerevisiae CNCM I-1077 (Lallemand SAS, Toulouse, France). Twenty multiparous, midlactation Holstein cows were assigned to two treatments in crossover design. Treatments were: live yeast on oyster meal capable of supplying a daily minimum of 1 × 10(10) CFU per cow or oyster meal top-dressed at 10 g to the morning meal. Diet contained (% of dry matter): 16.8% crude protein, 30.9% neutral detergent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Citrus pulp; Probiotic.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000300005

ACÚMULO DE CÁDMIO POR Saccharomyces cerevisiae  FERMENTANDO MOSTO DE MELAÇO
Provedor de dados: 63
Autores: PRADO-FILHO,L.G. do; DOMINGOS,R.N.; SILVA,S.M.G. da.
O presente trabalho visou o estudo do acúmulo de cádmio (Cd) por Saccharomyces cerevisiae, fermentando mosto de melaço com contaminações controladas em níveis sub-tóxicos do citado metal. As condições de fermentação foram similares às reinantes na produção industrial de etanol. O mosto, não esterilizado, continha 12% de açúcares redutores totais (ART) e pH 4,5. Para a contaminação controlada empregou-se dois sais de cádmio, cloreto e acetato e, quatro níveis de contaminação 0,5; 1,0; 2,0 e 5,0 mg Cd.kg-1 mosto. A inoculação do mosto foi executada com fermento de panificação (10% p/p). Após a fermentação (4 horas) foram determinados, porcentagem de fermento no vinho centrifugado e teor alcoólico. Na levedura separada foram determinados peso úmido, matéria...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Melaço; Metal pesado; Cádmio; Espectrofotômetria de absorção atômica.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161998000100020

Enriching nutritive value of cassava root by yeast fermentation
Provedor de dados: 63
Autores: Boonnop,Krisada; Wanapat,Metha; Nontaso,Ngarmnit; Wanapat,Sadudee.
Cassava (Manihot esculenta) is extensively cultivated throughout the tropics and subtropics regions due to its ability to grow in diverse soil conditions and minimal management. Experiments were made to study the cassava root fermentation by yeasts in order to enhance the nutritive value of their products (fresh pulp and chips). Both cassava chip (CC) and fresh cassava root pulp (FCR) samples were fermented by Saccharomyces cerevisiae in solid-liquid media fermentation conditions during 132 hours and dried at 30ºC. Products were analyzed for proximate composition, mineral composition, essential aminoacids and antinutrient content. There were increases (p &lt; 0.01) in protein (30.4% in CC and 13.5% in fermented cassava root -FCR) and fat contents (5.8% in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Fermentation; Cassava chip; Fresh cassava root pulp; Protein; Animal feed.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000500007

Thermolysed and active yeast to reduce the toxicity of aflatoxin
Provedor de dados: 63
Autores: Baptista,Antonio Sampaio; Horii,Jorge; Calori-Domingues,Maria Antonia; Glória,Eduardo Micotti da; Salgado,Jocelem Mastrodi; Vizioli,Mário Roberto.
Aflatoxins are hepatotoxic metabolites produced by Aspergillus flavus and A. parasiticus on a number of agricultural commodities. This research was carried out to evaluate the ability of thermolysed and active Saccharomyces cerevisiae to attenuate liver damage caused by aflatoxin. Diets were prepared containing 0 aflatoxin; 400 mug kg-1 aflatoxin; 400 mug kg-1 aflatoxin plus 1% of dehydrated active yeast, and 400 mug kg-1 aflatoxin plus 1% of thermolysed yeast. A bioassay with Wistar rats was conducted for 28 days, and body organs were weighted and analyses of the liver tissue of the animals were performed. The relative weight of heart, kidneys and liver from animals submitted to the different treatments did not show any difference, and liver tissue of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Aflatoxicosis; Bioremediation; Mycotoxins; Natural contamination.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000200008

Viabilidade celular de Saccharomyces cerevisiae em cultura mista com Lactobacillus fermentum
Provedor de dados: 63
Autores: OLIVEIRA-FREGUGLIA,R. M.; HORII,J.
O presente trabalho teve por objetivo avaliar os efeitos de L. fermentum em cultura mista com S. cerevisiae sobre a viabilidade celular da levedura, bem como outros parâmetros relacionados com os produtos metabólicos bacterianos. Os microrganismos foram cultivados individualmente em meio de caldo de cana-de-açúcar diluído e suplementado com extrato de levedura e peptona. A partir da mistura de ambas as culturas foi acompanhada a viabilidade celular da levedura em ensaios denominados: cultura mista ativa, bactéria inativada por esterilização e inativada por agentes antimicrobianos. Nos cultivos onde foi observada floculação, foi testada a ação de enzimas do grupo peptidohidrolases (papaína, bromelina e ficina). A cultura mista ativa apresentou redução da...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Viabilidade; Floculação; Saccharomyces cerevisiae; Lactobacillus fermentum.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161998000300022

Alterações na atividade e no perfil eletroforético da peroxidase em folhas de milho (Zea mays) e sorgo (Sorghum bicolor) tratadas com levedura (Saccharomyces cerevisiae)
Provedor de dados: 63
Autores: RONCATTO,M.C.; PASCHOLATI,S.F.
O trabalho foi desenvolvido com o objetivo de se verificar a influência de Saccharomyces cerevisiae na forma de produto comercial, fermento biológico fresco para panificação, na atividade e no padrão eletroforético da peroxidase em folhas de milho e sorgo. As preparações de Saccharomyces utilizadas foram representadas por suspensões de células e seus respectivos filtrados, autoclavados ou não. A análise dos extratos obtidos dessas gramíneas mostrou que suspensões de células e os filtrados dessas suspensões, tratadas termicamente ou não, foram efetivos em ocasionar alterações na atividade e padrão eletroforético da peroxidase em milho e sorgo. Além de se mostrarem termoestáveis, os extratos obtidos de células intactas ou homogeneizadas de S. cerevisiae...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Peroxidase; Eletroforese; Milho; Sorgo.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161998000300007

FUSÃO DE PROTOPLASTOS DE Saccharomyces cerevisiae AVALIADA POR FLOCULAÇÃO E PRODUÇÃO DE H2S
Provedor de dados: 63
Autores: MARTINS,C.V.B.; HORII,J.; PIZZIRANI-KLEINER,A.A.
Com o objetivo de obter linhagens de leveduras com características de importância para a indústria vinícola, empregou-se a técnica da fusão de protoplastos. A atenção foi dirigida a duas características: floculação e não produção de H2S. Uma vez que cada característica é de baixa frequência (1% cada) em leveduras vinícolas, torna-se pouco provável a ocorrência natural de uma linhagem com ambas as características. O cruzamento foi realizado via fusão de protoplastos pois as linhagens apresentaram incompatibilidade sexual. Inicialmente foram obtidos os mutantes auxotróficos contrastantes e posteriormente suas marcas foram avaliadas quanto à reversão e, em seguida foram determinadas suas curvas de crescimento. Obteve-se a protoplastização por tratamento com...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fusão de protoplastos; Sulfeto de hidrogênio; Floculação; Leveduras; Saccharomyces cerevisiae; Linhagens.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161998000100012

การใช้กากมันสำปะหลังจากการผลิตเอทานอลหมักยีสต์ เพื่อเป็นอาหารในโครีดนม
Provedor de dados: 106
Autores: Supakit Sunato; Virote Pattarajinda; Pronchai Lowilai; Ngarmnit Nontaso.
The ethanol production often uses cassava as energy source for their production. The waste product can help improve the quality before reuse as animal feed. The objective of this study was to determine the effect of fermented cassava ethanol byproducts with yeast (Saccharomyces cerevisiae) on feed efficiency and milk production of dairy cattle. Twelve Holstein Friesian crossbreds (405.4 ± 43.9 kg BW) with an average milk yield in 10.2 ±1.2 kg/d were used. The treatment were allocated into Randomize completely block design (RCBD) that consisted of four levels of fermented cassava ethanol byproducts with yeast as level as 0, 25, 35 and 45% of DM basis in total mixed ration (TMR) diet based on Ruzi grass as roughages source. The results showed that dry matter...
Tipo: PhysicalObject
Palavras-chave: Dairy cow; Lactating; Cassava ethanol byproducts; Saccharomyces cerevisiae; Feed fortification; Feed intake; Milk production; Milk composition; โคนม; ช่วงรีดนม; กากมันสำปะหลัง; ของเหลือจากการผลิต; เอทานอลหมักยีสต์; การให้อาหารสัตว์; สูตรอาหาร; ปริมาณการกินได้; การเจริญเติบโต; ผลผลิตน้ำนม; องค์ประกอบน้ำนม.
Ano: 2013
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5801

การศึกษาการเพิ่มระดับโปรตีนของกากมะพร้าวสดและแห้ง โดยกระบวนการหมักยีสต์และยูเรีย
Provedor de dados: 106
Autores: Narumon Somkuna; Jarous Sawangtap; Jiraprapa Rodjarkken; Surasak Uttarawichian.
The objective of this study was to compare the increasing protein level of fresh and dry coconut meal by urea-yeast (Saccharomyces cerevisiae) fermentation process. The design was 2 X 8 factorial in CRD : factor A composed of 2 factors (dry and fresh coconut meal) and factor B composed of 8 factors (day of fermentation: 5, 10, 15, 20, 25, 30, 35 and 40 days). The samples of each day of fermentation were collected for crude protein analysis. The data was analyzed by using Analysis of Variance (ANOVA). The results were shown that the protein level at the different days of fermentation was different in fresh and dry coconut meal (P&lt;0.05) which protein level of fresh coconut meal at day 10 was highest (34.40 %). While protein level in dry coconut meal was...
Tipo: PhysicalObject
Palavras-chave: Fresh coconut meal; Dry coconut meal; Coconut copra; Kernel meal; Fermentation process; Yeast; Saccharomyces cerevisiae; Urea; Animal feed; Crude protein level; กากมะพร้าวสด; กากมะพร้าวแห้ง; มะพร้าว; กระบวนการหมัก; ยีสต์; ยูเรีย; ระยะเวลาหมัก; อาหารสัตว์; โปรตีนหยาบ; การเพิ่มโปรตีน; ระดับโปรตีน.
Ano: 2014
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5742

การศึกษาการผลิตโปรตีนเซลล์เดียวจากเปลือกสับปะรด โดยใช้ยีสต์และบาซิลัสซัปติลิสเพื่อพัฒนาเป็นอาหารสัตว์
Provedor de dados: 106
Autores: Manat-Sanun Nopparatmaitree; Pornpan Seanphoom; Warangkana Kitpipit; Krittiya Lertchunhakiat.
This experiment was conducted to study on single cell protein production from pineapple peel by Sacharomyces cerevisiae and Bacillus subtilis as animal feedstuff. The experimental design was 4 x 3 factorial in CRD experiment with two factors. The factor A was comprised fermented period (0, 15, 30 and 45 day). While, the factor B was comprised of type of microorganism (Sacharomyces cerevisiae, Bacillus subtilis and Sacharomyces cerevisiae combined with Bacillus subtilis). The results showed that interaction between factor A and Factor B on incubation pH 3.88-4.16, temperature 28.62-29.78 C and yield 11.22-16.94 % were highly significantly (P&lt;0.01). For nutrients composition, the group was fermented Sacharomyces cerevisiae combined with Bacillus subtilis...
Tipo: PhysicalObject
Palavras-chave: Pineapple peel; Saccharomyces cerevisiae; Bacillus subtilis; Feedstuff; Single cell protein; Crude protein; Digestible protein; Pepsin enzyme; เปลือกสับปะรด; ยีสต์; บาซิลัส ซัสติลิส; การหมัก; การผลิตอาหารสัตว์; โปรตีนเซลล์เดียว; ค่าโปรตีน; คุณค่าทางโภชนะ; การย่อยโปรตีน; เอนไซม์เปปซิน.
Ano: 2013
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5803

Preliminary study of ethanol production from sorghum
Provedor de dados: 106
Autores: Siwawej, S.; Suraphanpisit, Y.
Palavras-chave: Sorghum; Ethanol; Production; Fermentation; Saccharomyces cerevisiae; Mold; Enzyme activity; ข้าวฟ่าง; เอทานอล; แป้งข้าวฟ่าง; ยีสต์; พันธุ์วาย-90; การผลิต.
Ano: 1981
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/4081

ผลของสายพันธุ์ยีสต์ต่อสมรรถนะการให้ผลผลิตในโคนม
Provedor de dados: 106
Autores: Vatsana Sirisan; Virote Pattarajinda; Kanit Vichitphan; Rattanaporn Leesing.
Saccharomyces cerevisiae as animal feed additive has been widely used in order to promote growth and production performances. Consequently, the studies to screening local yeast strains were conducted to compare the respond between S. cerevisiae and Kodameae ohmeri on feed intake, body weight change, milk production and milk composition. Sixteen Holstein Friesian crossbreds, 450 ± 50 kg BW average were used in this study. Animal were allocated into completely randomized design (CRD) to the following 4 treatments: control group, 109 cells/d of S. cerevisiae, 106 and 109 cells/d of K. ohmeri. The results showed that addition of K. ohmeri with 109 cells/d was increased dry matter intake, body weight change and fat production compared to 106 cells/d and control...
Tipo: PhysicalObject
Palavras-chave: Dairy cow; Yeast direct fed; Saccharomyces cerevisiae; Kodameae ohmeri; Feed supplements; Feed fortification; Dry matter intake; Body weight; Milk production; Milk composition; โคนม; ยีสต์; สายพันธุ์; การเสริมอาหารสัตว์; การให้อาหารสัตว์; การกินได้สิ่งแห้ง; สมรรถนะผลผลิต; ผลผลิตน้ำนม; องค์ประกอบน้ำนม; ปริมาณไขมันนม.
Ano: 2013
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5800

Aproveitamento do camu-camu (Myrciaria dubia) para produção de bebida alcoólica fermentada
Provedor de dados: 36
Autores: Maeda,Roberto Nobuyuki; Andrade,Jerusa Souza.
O elevado teor de ácido ascórbico no camu-camu (Myrciaria dubia McVaugh, Myrtaceae) desperta o interesse de extrativistas, agricultores e consumidores, e leva à necessidade de desenvolvimento de tecnologias adequadas para produção em terra firme e aproveitamento industrial do fruto. Este trabalho teve por objetivo verificar a adequação do camu-camu para a produção de bebida alcoólica fermentada, assim como o efeito do branqueamento do fruto e da incorporação da casca à polpa nas características nutricionais e sensoriais da bebida. Os frutos foram separados em quatro lotes, sendo dois branqueados (90 ºC por 7 min). Após a despolpa, as cascas de um lote de cada tratamento (com e sem branqueamento) foram incorporadas às respectivas polpas e avaliadas quanto à...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fruto da Amazônia; Composição química; Saccharomyces cerevisiae; Fermentação; Análise sensorial.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672003000300014

Diversity of yeasts during fermentation of cocoa from two sites in the Brazilian Amazon
Provedor de dados: 36
Autores: ALMEIDA,Silvana de F. Oliveira de; SILVA,Letícia R Carvalho; JUNIOR,Gilson Celso A. Chagas; OLIVEIRA,Guilherme; SILVA,Silvia Helena Marques da; VASCONCELOS,Santelmo; LOPES,Alessandra Santos.
ABSTRACT The purpose of this study was to identify the yeasts involved in spontaneous fermentation of cocoa from the Brazilian Amazon region. The fermentation process was carried out experimentally with cocoa seeds from two sites (Medicilândia and Tucumã), State of Pará, northern Brazil, during a six-day period. Totals of 44 yeasts were isolated from Medicilândia and 29 from Tucumã. Molecular identification was carried out by sequencing the D1/D2 region fragment of the rRNA 26S gene, expanded with universal primers for the NL1GC and LS2 eukaryotes. Pichia manshurica and Saccharomyces cerevisiae were identified in Medicilândia and five yeast species (Pichia fermentans, P. kudriavzevii, P. manshurica, S. cerevisiae and Zygosaccharomyces bailii) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Accession; Molecular identification; Cocoa beans; Pichia Manshurica; Saccharomyces cerevisiae; Theobroma cacao.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000100064

Hematological and biochemical responses of pirarucu (Arapaima gigas, Arapaimidae) fed with diets containing a glucomannan product derived from yeast and algae
Provedor de dados: 36
Autores: HOSHINO,Maria Danielle Figueiredo Guimarães; MARINHO,Renata das Graças Barbosa; PEREIRA,Diogle Farias; YOSHIOKA,Eliane Tie Oba; TAVARES-DIAS,Marcos; OZORIO,Rodrigo Otavio de Almeida; RODRIGUEZ,Anselmo Fortunato Ruiz; RIBEIRO,Ricardo Amaral; FARIA,Fernando Sérgio Escócio Drummond Viana de.
ABSTRACT The hematological and biochemical responses of pirarucu fingerlings (Arapaima gigas) fed with diets containing different concentrations of a glucomannan product derived from yeast and algae were evaluated in order to ascertain the effect of these diets on fish physiology. Four treatments were conducted, with three replications, with 12 fish in each tank. The product evaluated (MycosorbA+(r)) was incorporated into the commercial diet, at four concentrations: 0, 1, 2 and 4 g.kg-1, called M0%, M0.1%, M0.2% and M0.4%, respectively. After 45 days of feeding, blood samples from six fish in each replicate were collected to perform the analyses. Their weight and length were determined to calculate the condition factor and weight gain, but no differences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorella vulgaris; Saccharomyces cerevisiae; Leukocytes; Albumin.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672017000200087

The impact of dietary dry yeast on lactation curves of primiparous and multiparous Saanen goats
Provedor de dados: 2
Autores: Gomes, Ludmila Couto; Alcalde, Claudete Regina; Souza, Rodrigo de; Molina, Bruna Susan de Lábio; Lourenço, Daniela Andressa de Lino; Martins, Elias Nunes.
Twenty-four Saanen goats (fifteen multiparous and nine primiparous) during their lactation period were used, from the kidding until the 200th day of lactation; and were distributed in a completely randomized design and in a factorial arrangement (3 diets x 2 parity orders). The diets were composed of either soybean meal (SBM), soybean meal plus inactive dry yeast (SMDY) or inactive dry yeast (DY) used as the protein source. The analyses of milk yield, body weight and dry matter intake (DMI) were made through the Bayesian methodology. For construct the lactation curves were used the Wood’s nonlinear model. The inclusion of inactive dry yeast in the diets reduced the rate of yield increase until the lactation peak for primiparous goats, but for multiparous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5.04.05.00-4 bayesian methodology; Milk productivity; Wood’s nonlinear model; Saccharomyces cerevisiae.
Ano: 2014
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/24446

Desempenho, digestibilidade, composição corporal e morfologia intestinal de pintos de corte recebendo dietas contendo levedura de cana-de-açúcar - doi: 10.4025/actascianimsci.v33i1.10787
Provedor de dados: 2
Autores: Lopes, Claudia da Costa; Universidade Federal Rural de Pernambuco; Rabello, Carlos Bôa-Viagem; Universidade Federal Rural de Pernambuco; Silva Júnior, Valdemiro Amaro da; Universidade Federal Rural de Pernambuco; Holanda, Mônica Calixto Ribeiro de; Universidade Federal Rural de Pernambuco; Arruda, Emmanuele Maria Florêncio de; Universidade Federal Rural de Pernambuco; Silva, Jaqueline de Cássia Ramos da; Universidade Federal Rural de Pernambuco.
Objetivou-se avaliar o desempenho, a digestibilidade das dietas, a retenção corporal de nutrientes e o desenvolvimento da mucosa intestinal de pintos de corte de 1 a 8 dias de idade, alimentados com dietas contendo níveis crescentes de levedura de cana-de-açúcar. Utilizou-se 300 pintos de corte, com um dia de idade, distribuídos em delineamento inteiramente casualizado, composto de cinco tratamentos e seis repetições de 10 aves. Os tratamentos consistiram de níveis crescentes de 0; 1,25; 2,5; 3,75 e 5,0% de inclusão de levedura. Não houve efeito da levedura sobre o desempenho zootécnico. Houve redução no coeficiente de metabolizabilidade da energia bruta, na energia metabolizável aparente e a aparente corrigida com os níveis crescentes. O nível de 2,09%...
Tipo: Pesquisa de campo; análise laboratorial
Palavras-chave: 5.04.00.00-2 avaliação nutricional; Frango de corte; Vilosidades intestinais; Retenção de nutrientes; Saccharomyces cerevisiae nutritional evaluation; Broiler; Intestinal villi; Nutrient retention; Saccharomyces cerevisiae.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/10787

Levedura ou monensina na dieta de bovinos e bubalinos sobre a fermentação ruminal e eficiência microbiana - doi: 10.4025/actascianimsci.v33i4.11264
Provedor de dados: 2
Autores: Zeoula, Lúcia Maria; Universidade Estadual de Maringá; Beleze, Juliano Ricardo Fontanini; Universidade Estadual de Maringá; Maeda, Emilyn Midori; Universidade Estadual de Maringá; Simioni, Fabiano Luís; Universidade Estadual de Maringá; Geron, Luiz Juliano Valério; Universidade Estadual de Maringá; Rigolon, Luiz Paulo; Universidade Estadual de Maringá.
Objetivou-se avaliar o uso da monensina sódica (Rumensin&amp;reg;) e da levedura viva (Saccharomyces cerevisiae - Beef - sacc&amp;reg;) sobre a concentração de ácidos graxos de cadeia curta (AGCC), amônia e pH ruminal, e eficiência microbiana em bubalinos e bovinos, alimentados com dietas com 50% de concentrado. Foram utilizados três búfalos e três bovinos portadores de cânulas no rúmen e no duodeno, distribuídos em dois quadrados Latinos, com arranjo fatorial 3 x 2. As concentrações de AGCC, amônia e o pH ruminal foram determinados a cada 2h. Os fluxos de digesta e proteína microbiana no duodeno foram determinados, respectivamente, por meio dos indicadores cinza insolúvel em ácido e as bases purinas. Não houve efeito dos aditivos e das espécies para a...
Tipo: Pesquisa aplicada
Palavras-chave: Ciências Agrárias  Zootecnia Ácidos graxos voláteis; Amônia; Ionóforo; Saccharomyces cerevisiae short chain fatty acids; Ammonia; Ionophore; Saccharomyces cerevisiae.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/11264

Effect of probiotics and Yucca schidigera extract supplementation on broiler meat quality
Provedor de dados: 2
Autores: Benamirouche, Karima; Baazize-Ammi, Djamila; Hezil, Nadia; Djezzar, Reda; Niar, Abdellatif; Guetarni, Djamel.
The current study investigated the effect of dietary supplementation with probiotics and Yucca schidigera extract on physicochemical parameters, proximate composition, mineral content and fatty acid profile of broiler breast and thigh muscles. In total, 240 one-day old broilers were randomly allocated into two dietary treatments groups: 1) Control (basal diet), 2) experimental (basal diet with two probiotics Pediococcus acidilactici and Saccharomyces cerevisiae and Yucca schidigera extract). The results showed that the pH value was higher in the experimental group than in the control group (p &amp;lt; 0.05). However, drip, cook and thaw losses were not influenced by dietary treatment (p &amp;gt; 0.05). A significant increase in protein, Fe, Zn, Na, P and a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast; Meat quality; Pediococcus acidilactici; Saccharomyces cerevisiae; Thigh.
Ano: 2020
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/48066

Digestibilidade total e balanço de nitrogênio em cabritos recebendo rações contendo levedura seca - doi: 10.4025/actascianimsci.v33i3.10175
Provedor de dados: 2
Autores: Freitas, Hanna Sakamoto; Universidade Estadual de Maringá; Alcalde, Claudete Regina; Universidade Estadual de Maringá; Lima, Luciano Soares de; Universidade Estadual de Maringá; Zeoula, Lúcia Maria; Universidade Estadual de Maringá; Costa, Leonardo Salles Esteves da; Universidade Estadual de Maringá; Lima, Larissa Ribas de; Universidade Estadual de Maringá.
O experimento foi conduzido com o objetivo de avaliar a ingestão, digestibilidade da matéria seca e dos nutrientes, o balanço de nitrogênio e estimar os nutrientes digestíveis totais de rações com a inclusão da levedura seca (0, 25, 50, 75 e 100% da MS) em substituição ao farelo de soja nas rações para cabritos. Foram utilizados cinco cabritos SRD alojados em gaiolas metabólicas distribuídos em delineamento quadrado latino 5 x 5. As ingestões de matéria seca e de carboidratos totais não foram alteradas com a inclusão de levedura, no entanto, para a ingestão de matéria orgânica, proteína bruta, extrato etéreo e fibra em detergente neutro foi observado efeito quadrático. A digestibilidade do extrato etéreo apresentou efeito linear decrescente, porém, para...
Tipo: Pesquisa de campo; análise laboratorial
Palavras-chave: 5.04.03.00-1; 5.04.03.01-0; 5.04.03.02-8 Digestão; Ingestão; Nitrogênio retido; Saccharomyces cerevisiae; Subproduto da cana-de-açúcar Digestion; Intake; Nitrogen retention; Saccharomyces cerevisiae; Sugar cane byproduct.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/10175

Simultaneous co-Saccharification and Fermentation of Sago Hampas for Bioethanol Production
Provedor de dados: 111
Autores: Vincent, Micky A.
Abundance of lignocellulosic biomass provides a good solution to the demands of energy crops in producing biofuel like biodiesel and bioethanol. In this study, bioethanol was produced from sago hampas via the Simultaneous co-Saccharification and Fermentation (Sc-SF) process, at 2.5% and 5.0% (w/v) solid loadings. The processing step in Sc-SF is virtually similar to that of Simultaneous Saccharification and Fermentation (SSF). However, during Sc-SF, two enzymes, amylase and cellulase, were added for the co-saccharification of sago starch and fiber. In addition, Saccharomyces cerevisiae was used to ferment the sugars in the hydrolysates. The Sc-SF samples were analyzed for carbohydrate residues, ethanol and acetic acid using the dinitrosalicylic (DNS) acid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofuel  Biorenewable Resources Technology Bioethanol; Sago hampas; Simultaneous co-Saccharification and Fermentation (Sc-SF); Amylase; Cellulase; Saccharomyces cerevisiae; HPLC.
Ano: 2015
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/2921

Production of ethanol by Saccharomyces cerevisiae and Zymomonas mobilis Coimmobilized: proposal for the use of organic waste
Provedor de dados: 115
Autores: Ruiz-Marín,Alejandro; Canedo-López,Yunuén; Narváez-García,Asteria; Robles-Heredia,J. Carlos.
Abstract: Fermentation technologies were developed to improve the production of ethanol and an alternative is the immobilization technology, which offers advantages in comparison with free cells culture, such as the possibility of efficiently incorporating symbiotic bacteria in the same matrix. The aim of this study was to evaluate, in equivalent medium, the use of mango (Mangifera indica) waste to produce ethanol. The study compared the production of ethanol by using Zymomonas mobilis and Saccharomyces cerevisiae in free cells culture, immobilized and coimmobilized in calcium alginate in a culture medium equivalent to mango juice. It also included an evaluation of the effect of glucose concentration on productivity in a coimmobilized system. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mangifera indica; Immobilization; Coimmobilization; Ethanol; Zymomonas mobilis; Saccharomyces cerevisiae.
Ano: 2016
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952016000500551

Efecto del selenio y cromo orgánicos y Saccharomyces cerevisiae en la degradación in situ de la dieta, fermentación ruminal y crecimiento de borregos
Provedor de dados: 115
Autores: Reséndiz-Hernández,Miriam; Bárcena-Gama,José R.; Crosby-Galván,María M.; Cobos-Peralta,Mario; Herrera-Haro,José; Hernández-García,Pedro A.; Carreón-Luna,Lorenzo.
El cromo (Cr) y el selenio (Se) son micro minerales esenciales para rumiantes, modifican el metabolismo y su adición en las dietas puede afectar la degradación del alimento y la fermentación en rumen y, por tanto, la respuesta productiva de los animales. El objetivo del presente estudio fue evaluar el efecto de Se (0.90 mg kg-1) + Cr (1.4 mg kg-1) ambos quelados con levadura inerte en Biotecap® (Se+Cr orgánicos) y de Saccharomyces cerevisiae (SC; cepa 7907) en: 1) degradación in situ de la MS, FDN, FDA y PC; 2) pH, AGV y N-NH3 en rumen; 3) consumo de MS, ganancia diaria de peso (GDP), conversión alimenticia (CA), así como en contenido de grasa de la canal de borregos. Los tratamientos fueron dietas: 1) testigo, sin Se+ Cr ni SC; 2) testigo+ Se+ Cr; 3)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Minerales orgánicos; Saccharomyces cerevisiae; Degradación in situ; Borregos.
Ano: 2012
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952012000800001

Aislamiento, selección e identificación de levaduras Saccharomyces spp. nativas de viñedos en Querétaro, México
Provedor de dados: 115
Autores: Miranda-Castilleja,Dalia E.; Ortiz-Barrera,Eunice; Arvizu-Medrano,Sofía M.; Ramiro-Pacheco,Juan; Aldrete-Tápia,Jesús A.; Martínez-Peniche,Ramón Á.
Querétaro es el segundo estado productor de vinos en México, pero la comercialización es difícil por la falta de calidad y tipicidad. Una alternativa es tener levaduras nativas seleccionadas para aumentar el potencial de los mostos y dar originalidad a los vinos. El objetivo del presente estudio fue el aislamiento, selección con base en su potencial enológico e identificación de levaduras Saccharomyes nativas de viñedos queretanos. Las cepas fueron aisladas en 2012 en el laboratorio de fermentaciones y fisiología de frutos de la Universidad Autónoma de Querétaro, México, durante la fermentación espontánea de mostos de uvas maduras recolectadas aleatoriamente dentro de tres viñedos queretanos, diferenciando las Saccharomyces en medio lisina. Su tolerancia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Selección de levaduras; Vinificación; Saccharomyces cerevisiae; Saccharomyces paradoxus.
Ano: 2015
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952015000700005

Efeito dos ácidos fórmico e propiônico sobre a produção de alcoóis superiores durante a fermentação alcoólica
Provedor de dados: 43
Autores: Gutierrez,L.E.
Foi estudado o efeito de concentrações de 250, 500 e 1000 ppm dos ácidos fórmico e propiônico sobre a produção de etanol e alcoóis superiores produzidos pela fermentação alcoólica de mosto sintético. Não foram detectadas diferenças significativas entre os tratamentos tanto para o ácido fórmico como para o ácido propiônico, embora uma redução não significativa no teor do álcool isoamílico foi observada com a dose de 1000 ppm dos ácidos fórmico e propiônico.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ácido fórmico; Ácido propiônico; Alcoóis superiores; Fermentação alcoólica; Saccharomyces cerevisiae.
Ano: 1988
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0071-12761988000100023

Efeito da adição de sulfito sobre a produção de alcoóis superiores durante a fermentação alcoólica
Provedor de dados: 43
Autores: Gutierrez,L.E.
Foi estudado o efeito de quatro concentrações de sulfito (19, 69, 119, 219 ppm) em mostos de melaço de cana em pH 4,O e o efeito do pH de mosto sulfitado sobre a produção de etanol, acetaldeído e os alcoóis n-propílico, isobutílico e isoamílico durante a fermentação alcoólica por Saccharomyces cerevisiae. Não foram detectadas diferenças significativas entre os teores de etanol dos mostos sulfitados. Houve aumento significativo no teor de acetaldeído e redução significativa nos teores de alcoóis superiores com o aumento da concentração de sulfito. A elevação do pH do mosto sulfitado provocou aumento significativo nos teores de acetaldeído e alcoóis superiores não afetando a produção de etanol.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sulfito; Fermentação alcoólica; Óleo fusel; Alcoóis superiores; Saccharomyces cerevisiae.
Ano: 1988
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0071-12761988000100022

Capacidade fermentativa de Saccharomyces cerevisiae enriquecida com ácidos graxos
Provedor de dados: 43
Autores: Gutierrez,L.E.; Annicchino,A.V.K.O.; Lucatti,L.
Levedura de panificaçao (Saccharomyces cerevisiae) foi obtida anaerobicamente (sem e com adição dos ácidos graxos palmítico, oleico e linoleico) e aerobicamente e utilizada em ensaios de fermentação com 14% e 16% de sacarose a 32°C. Não houve diferenças significativas, quanto a viabilidade celular, entre os tratamentos das leveduras com ácido oleico, ácido linoleico e aerobicamente (as quais foram ricas em palmitoleico e oleico). As leveduras enriquecidas com ácido palmítico e anaeróbicas apresentaram maior redução na viabilidade do que com ácidos graxos insaturados. Foi observado um aumento na produção de ácido pirúvico e uma redução nos álcoois superiores com a redução da viabilidade celular.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ácidos graxos; Saccharomyces cerevisiae; Fermentação alcoólica.
Ano: 1990
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0071-12761990000200017

Efeito da adição de sulfato de amônio sobre a produção de ácido succínico durante a fermentação alcoólica
Provedor de dados: 43
Autores: Gutierrez,L.E.
A produção de ácido succínico por leveduras durante a fermentação alcoólica de mosto de melaço suplementado com 25, 50 e 100 ppm de nitrogênio na forma de sulfato de amônio foi determinada por cromatografia em fase gasosa. A adição de nitrogênio amoniacal não afetou significativamente a produção de álcool etílico. Houve redução significativa no teor de ácido succínico com o aumento da quantidade de nitrogênio adicionada.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ácido succínico; Sulfato de amônio; Fermentação alcoólica; Leveduras; Saccharomyces cerevisiae.
Ano: 1988
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0071-12761988000100027

Effect of inactive dry yeast from sugar cane as protein source on rumen fermentation in Saanen goats
Provedor de dados: 45
Autores: Lima,L.S.; Alcalde,C.R.; Gomes,L.C.; Souza,R.; Rigolon,L.P.; Nakamura,C.V.
Five castrated and ruminally cannulated Saanen goats (±48.19kg) were used to evaluate intake, digestibility and rumen fermentation parameters of diets with inactive dry yeast as a soybean meal substitute (0, 25, 50, 75 and 100%). Goats were randomly assigned to a 5×5 (five levels of dry yeast x five periods) Latin square design. Diets were composed of corn silage (40%), ground corn, soybean meal and/or dry yeast and mineral supplement. The intake and digestibility of DM, OM, CP, NDF and TC were not influenced by the treatments. However, EE intake showed negative linear effect. The TDN content did not change with the inclusion of dry yeast in the diets. The pH, N-NH3 concentration and rumen short-chain fatty acids content did not differ among diets. Rumen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ammonia; Digestibility; Goats; Saccharomyces cerevisiae.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000100021

Levedura seca integral na alimentação de vacas lactantes
Provedor de dados: 45
Autores: Freitas,D.R.; Campos,J.M.S.; Marcondes,M.I.; Valadares Filho,S.C.; Franco,M.O.; Martins,E.C.; Rodrigues,B.M.C.; Oliveira,A.S.
Os efeitos da inclusão de levedura seca de cana-de-açúcar (Saccharomyces cerevisiae) na dieta de vacas lactantes foram avaliados com base no fornecimento de quatro dietas constituídas de diferentes níveis de substituição do farelo de soja pela levedura de cana-de-açúcar (0,00; 0,33; 0,67 e 1,00kg/kg de MS). O delineamento utilizado foi o quadrado latino 4 × 4. Não houve efeito dos tratamentos sobre o consumo de MS, MO, EE, FDNi e NDT, porém ocorreu redução linear no consumo de PB e FDNcp e aumento do consumo de CNF. Diferenças estatísticas não foram verificadas para os coeficientes de digestibilidade aparente da MS, MO, PB, CNF e NDT; no entanto os coeficientes do FDNcp e do EE decresceram linearmente. Não foi observado efeito sobre a produção e eficiência...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ingestão; Performance; Saccharomyces cerevisiae; Vacas leiteiras.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000100211

Carcass quality of feedlot finished steers fed yeast, monensin, and the association of both additives
Provedor de dados: 45
Autores: Gomes,R.C.; Leme,P.R.; Silva,S.L.; Antunes,M.T.; Guedes,C.F.
To evaluate the effects of the supplementation of feed additives on carcass quality in beef cattle, 72 Nellore steers (339.5kg, 20-month old) were feedlot finished and fed for 91 days one of the following diets: 1) control with no additives; or added of 2) live yeast culture; 3) monensin; or 4) the association of both additives. After slaughter, renal, pelvic, and inguinal fat and hot carcass weights were recorded and carcass was split into muscle, bone, and trimmable fat. Carcass Longissimus muscle area and subcutaneous fat thickness at the 12th rib were measured and steaks of Longisimus muscle were taken to determine meat color, shear force, drip, and cooking losses. Yeast increased carcass dressing percentage but there were no effects on hot carcass...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Steer; Direct-fed microbial; Ionophores; Retail product yield; Shear force; Saccharomyces cerevisiae.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000300018

Energia metabolizável da levedura de cana-de-açúcar submetida a diferentes temperaturas e tempos de secagem para frangos de corte e poedeiras
Provedor de dados: 45
Autores: Lopes,C.C.; Rabello,C.B.V.; Dutra Júnior,W.M.; Medeiros,W.R.L.; Silva Júnior,R.V.; Custódio,L.R.; Lira,R.C.; Ludke,M.C.M.M.; Barbosa,E.N.R.; Lima,T.S.
Objetivou-se determinar a temperatura e o tempo de secagem por rolos rotativos, aos quais a, levedura de cana-de-açúcar é submetida que permitam seu melhor aproveitamento energético por galinhas poedeiras e frangos de corte. Para isso foram realizados três ensaios de metabolismo para determinar os valores de energia metabolizável aparente (EMA), aparente corrigida para nitrogênio (EMAn) e os coeficientes de metabolizabilidade aparente da matéria seca (CMMS) e da energia bruta (CMEB). O primeiro ensaio foi conduzido com galinhas poedeiras (E1), o segundo com frangos de corte (E2) em crescimento e o terceiro com frangos de corte em diferentes idades (E3). Nos ensaios E1 e E2 os tratamentos consistiram em uma dieta referência, milho e farelo de soja, e cinco...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alimento alternativo; Digestibilidade; Processamento; Saccharomyces cerevisiae; Valor energético.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000601711

Adição da parede celular de levedura (Saccharomyces cerevisiae) na dieta para frangos de corte
Provedor de dados: 45
Autores: Barroso,D.C.; Vieira,A.A.; Lima,C.A.R.; Trindade,B.S; Gomes,A.V.C.; Souza,M.M.S.; Corrêa,G.S.S.
Os efeitos da parede celular de Saccharomyces cerevisiae (PCSc) na dieta para frangos foram avaliados com base nos tratamentos: dieta referência com avilamicina (AV); dieta referência (DR); DR com 0,1% de PCSc (PCSc0,1); DR com 0,2% de PCSc (PCSc0,2); DR com 0,3% de PCSc (PCSc0,3). O delineamento foi o de blocos ao acaso, com cinco tratamentos e seis repetições de 10 aves, totalizando 300 frangos, machos. No período de nove a 21 dias de idade, o tratamento PCSc0,3 influenciou negativamente no ganho de peso (631g), e no período de 34 a 39 dias, o tratamento PCSc0,1 proporcionou melhor ganho de peso (461g) em relação ao tratamento com o antimicrobiano. O maior peso vivo ao abate, 2571g, foi obtido no tratamento PCSc0,1. Os pesos absolutos de sobrecoxa e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Frangos de corte; Prebiótico; Saccharomyces cerevisiae.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000400029

The Role of Tyrosine 207 in the Reaction Catalyzed by Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase
Provedor de dados: 48
Autores: Andrade,Cherie; Sepulveda,Carolina; Cardemil,Emilio; Jabalquinto,Ana M.
The functional signifcance of tyrosine 207 of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase was explored by examining the kinetic properties of the Tyr207Leu mutant. The variant enzyme retained the structural characteristics of the wild-type protein as indicated by circular dichroism, intrinsic fuorescence spectroscopy, and gel-exclusion chromatography. Kinetic analyses of the mutated variant showed a 15-fold increase in Km CO2, a 32fold decrease in Vmax, and a 6-fold decrease in Km for phosphoenolpyruvate. These results suggest that the hydroxyl group of Tyr 207 may polarize CO2 and oxaloacetate, thus facilitating the carboxylation/decarboxylation steps.
Tipo: Journal article
Palavras-chave: Phosphoenolpyruvate carboxykinase; Saccharomyces cerevisiae; CO2 interaction.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200007

Sortin2 enhances endocytic trafficking towards the vacuole in Saccharomyces cerevisiae
Provedor de dados: 48
Autores: Vásquez-Soto,Beatriz; Manríquez,Nicolás; Cruz-Amaya,Mirna; Zouhar,Jan; Raikhel,Natasha V; Norambuena,Lorena.
BACKGROUND: A highly regulated trafficking of cargo vesicles in eukaryotes performs protein delivery to a variety of cellular compartments of endomembrane system. The two main routes, the secretory and the endocytic pathways have pivotal functions in uni- and multi-cellular organisms. Protein delivery and targeting includes cargo recognition, vesicle formation and fusion. Developing new tools to modulate protein trafficking allows better understanding the endomembrane system mechanisms and their regulation. The compound Sortin2 has been described as a protein trafficking modulator affecting targeting of the vacuolar protein carboxypeptidase Y (CPY), triggering its secretion in Saccharomyces cerevisiae. RESULTS: A reverse chemical-genetics approach was used...
Tipo: Journal article
Palavras-chave: Endocytosis; Sortin2; Trafficking modulator; Saccharomyces cerevisiae.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100039

Residual polysaccharides from fungi reduce the bacterial spot in tomato plants
Provedor de dados: 53
Autores: Aguiar,Tarsis; Luiz,Caroline; Rocha Neto,Argus Cezar; Di Piero,Robson Marcelo.
Abstract Polysaccharides from fungal wastes were partially characterized and evaluated for their protective effects against bacterial spot caused by Xanthomonas gardneri on four tomato cultivars: Santa Cruz Kada, Natália, BRS Sena and Forty. The polysaccharides were extracted from spent mushroom substrate of Pleurotus ostreatus, residual brewery yeast (Saccharomyces cerevisiae), and basidiocarps discarded from Lentinula edodes production. These polysaccharides were characterized for total carbohydrates, phenolics and proteins content, pH, scatter intensity, conductivity, Zeta potential, DPPH scavenging assay and infrared spectroscopy. The effects of time interval between treatment and inoculation (4 or 7 days) and polysaccharide concentrations (0.5 or 1.5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Induced resistance; Lentinula edodes; Pleurotus ostreatus; Saccharomyces cerevisiae; Solanum lycopersicon; Xanthomonas gardneri.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052018000200299

Fermentation capacity of Saccharomyces cerevisiae cultures
Provedor de dados: 52
Autores: Alencar,Elvira Maria Bezerra de; Souza-Motta,Cristina Maria de; Walter,Bruno Souza; Santos,Rejane Maria Pessoa; Marques,Olga Martins; Queiroz,Lusinete Aciole de.
This work aimed at the assessment of fermentative capacity of original diploid, monocellular haploid and recuperated diploid cultures of S. cerevisiae in sterilized sugar-cane wort. Twenty eight cultures were analyzed, four being original diploids (URM-4420, Itaiquara Ferment FIT, Lallemand Ferment FLA and Wild Ferment SEL); 12 monocellular haploids from original ones and 12 recuperated diploids from the monocells. The ethanol percentage ranged from 1.7 to 6.2% and the unfermentable reducing sugars from 0.45 to 0.50g/100mL. The highest ethanol percentages were produced by the monocellular cultures URM-MH3 (4.8%) in 12 h and SEL-MH1 (6.2%) in 24 h, corresponding to the productivity values of 3.15 and 2.03 g.L-1.h-1, respectively. The recuperated diploids...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Monocellular; Fermentation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400004

Effect of method of secondary fermentation and type of base wine on physico-chemical and sensory qualities of sparkling plum wine
Provedor de dados: 52
Autores: Joshi,Vinod K; Sharma,Sanjeev K; Goyal,Ravinder K; Thakur,Narayan S.
Plum base wines prepared with potassium metabisulphite or sodium benzoate were converted into sparkling wine, either by `Methode Champenoise' or tank method with artificially carbonated wine serving as a control. In both the secondary fermentation methods ethanol and low temperature acclimatized yeast; Saccharomyces cerevisiae UCD-595 with optimized sugar (1.5%) and di-ammonium hydrogen phosphate (0.2%) were used. Both methods of sparkling wine production and the type of base wine affected the physico-chemical and sensory characteristics of the sparkling wine produced. In the secondary fermented wines, most of the physico-chemical characteristics were altered compared to that of artificially carbonated wines except volatile acidity, methanol, propanol and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plum; Prunus salicina L.; Sparkling wine; Potassium metabisulphite; Sodium benzoate; Saccharomyces cerevisiae.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300008

Effect of raffinose and ultrasound pulses on invertase release by free and immobilized Saccharomyces cerevisiae in loofa (Luffa cylindrica) sponge
Provedor de dados: 52
Autores: Marques,Leila Larisa Medeiros; Buzato,João Batista; Celligoi,Maria Antonia Pedrine Colabone.
This study investigated the effect of raffinose and ultrasound pulses on invertase release from free S. cerevisiae and S. cerevisiae immobilized in Luffa cylindrica. The free cell culture was submitted to 2% raffinose pulse and irradiated for 2 minutes at 0.12 and 0.46 h-1 dilution rates. The immobilized cell culture was submitted to raffinose pulse and irradiated for 1, 2 and 4 minutes, at 0.10 h-1 dilution rate. In immobilized cells, the raffinose pulse increased the invertase activity from 5.38 to 7.27 U/mg. Ultrasound application in free cell culture at the 0.12 h-1 dilution rate gave the best results. The activity varied from 25.08 to 29.38 U/mg while the increase in immobilized cells was from 5.22 to 9.70 U/mg when sonicated for two minutes. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Invertase; Luffa cylindrica; Raffinose; Saccharomyces cerevisiae; Ultrasound.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700003

Bioethanol production from sweet potato (Ipomoea batatas L.) flour using co-culture of Trichoderma sp. and Saccharomyces cerevisiae in solid-state fermentation
Provedor de dados: 52
Autores: Swain,Manas Ranjan; Mishra,Jyoti; Thatoi,Hrudayanath.
The aim of this work was to study the optimiation of co-culturing of Trichoderma sp. and Saccharomyces cerevisiae (1:4 ratio) on sweet potato (Ipomoea batatas L.) flour (SPF) for the production of bio-ethanol in solid-state fermentation (SSF). Maximum ethanol (172 g/kg substrate) was produced in a medium containing 80% moisture, ammonium sulphate 0.2%, pH 5.0, inoculuted with 10% inoculum size and fermented at 30ºC for 72h. .Concomitant with highest ethanol concentration, maximum ethanol productivity (2.8 g/kg substrate/h), microbial biomass (23×10(8) CFU/ g substrate), ethanol yield (47 g/100g sugar consumed) and fermentation efficiency (72%) were also obtained under these conditions. Cell interaction was observed familiar between the viable cells of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichoderma sp.; Saccharomyces cerevisiae; Sweet potato flour; Solid state fermentation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200002

Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification
Provedor de dados: 52
Autores: Silva,Gildo Almeida da; Bernardi,Taís Letícia; Schaker,Patrícia Dayane Carvalho; Menegotto,Morgana; Valente,Patricia.
The purpose of this work was to study a rapid yeast DNA extraction by boiling and freeze-thawing processes without using chemical reagents or any purification procedures, to obtain a high grade PCR-product. A specific DNA fragment of the 18S region of Dekkera bruxellensis and Saccharomyces cerevisiae was chosen. The described boiling and freeze-thawing protocols generated the PCR-grade product preparations and could be used to process many samples. The amplification of the fragments could be observed after 30 and 35 cycles. These processes of extraction without using any kind of chemical reagents, especial water, and purification procedures proved to be efficient, reproducible, simple, fast, and inexpensive.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Boiling; Freeze-thawing; DNA extraction; Dekkera bruxellensis; Saccharomyces cerevisiae.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200020

Cells of yeasts adhered in corn grains and the storage perspective for use as probiotic
Provedor de dados: 52
Autores: Baptista,Antonio Sampaio; Horii,Jorge; Piedade,Sonia Maria de Stefano.
Yeasts were applied to corn grains, containing 16 or 20% moisture, at concentrations of 1 and 2%. The cellular viability was assayed at 0, 15, 30, 90 and 110 days. The cellular viability did not differ statistically among the treatments up to 30 days of storage, with the median viability of 89.10%. The average viability found at 90 days (72.20%) was lower than in the first three storage periods. After 110 days, the average viability was 61.14%. In conclusion, since yeast cells were viable up to 110 days in storage on corn grains, these can be used as a vehicle for the application of yeast as a probiotic.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cellular viability; Saccharomyces cerevisiae; Storage; Corn grain.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200012

Glutathione production using magnetic fields generated by magnets
Provedor de dados: 52
Autores: Santos,Lucielen Oliveira dos; Gonzales,Tatiane Araujo; Úbeda,Beatriz Torsani; Alegre,Ranulfo Monte.
The objective of this work was to study the production of GSH by Saccharomyces cerevisiae ATCC 7754 in a fermentor (5 L) using a cell recycle system with magnets. The fermentation conditions were 20°C, 500 rpm, 5% (v/v) of inoculum, pHinitial 5, 1.1 vvm aeration and total fermentation time of 72 h. The time of application of MF ranged from 24, 48 or 72 h. In comparison to the control experiment, the best results were obtained with 72 h of application of MF. The cell concentration reached 19.5 g/L and GSH concentration was 271.9 mg/L that corresponded to an increase of 2.63 and 32.1% compared to the control experiment, respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutathione; Magnetic field; Saccharomyces cerevisiae; Batch fermentation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600016

Comparison between two selected Saccharomyces cerevisiae strains as fermentation starters in the production of traditional cachaça
Provedor de dados: 52
Autores: Gomes,Fátima de Cássia Oliveira; Araújo,Roberta Amália de Carvalho; Cisalpino,Patrícia Silva; Moreira,Elizabeth Spangler Andrade; Zani,Carlos Leomar; Rosa,Carlos Augusto.
Two Saccharomyces cerevisiae strains were tested as the starter yeasts in a traditional cachaça distillery. The strains used were S. cerevisiae UFMG-A829, isolated from a cachaça fermentation process, and S. cerevisiae K1-V1116, obtained from the wine industry. The permanence of each strain in the fermentation must was determined by RAPD (Random Amplified Polymorphic DNA)-PCR, with primer M13. Both yeast strains were prevalent in the vats for approximately 30 days. Indigenous non-Saccharomyces and indigenous S. cerevisiae strains were isolated in lower counts during the fermentation period. Indigenous S. cerevisiae strains were molecularly distinct when compared to the starter yeasts. The two yeasts appeared promising starter yeasts in the fermentation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cachaça; Saccharomyces cerevisiae; Starter strains; Fermentation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200023

The correlation between the central carbon metabolic flux distribution and the number of shared enzyme regulators in Saccharomyces cerevisiae
Provedor de dados: 52
Autores: Zhou,Xiangfei; Liu,Lunxian; Shang,Chuanyu; Xu,Haifeng; Ding,Chao; Liu,Qian; Yi,Yin.
The central carbon metabolic system is the upstream energy source for microbial fermentation. In addition, it is a master switch for increasing the production of metabolites and an important part of the microbial metabolic network. Investigation into the relationship between genes, environmental factors, and metabolic networks is a main focus of systems biology, which significantly impacts research in biochemistry, metabolic engineering, and synthetic biology. To this end, the central carbon metabolic flux under a variety of growth conditions or using strains with various genetic modifications was previously measured in Saccharomyces cerevisiae using 13C tracer technology. However, the measured values were not integrated and investigated further. In this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Central carbon metabolic flux; Metabolic networks and pathways; Gene expression regulation; Gene environment interaction; Enzyme transcriptional regulator.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100415

Chemical inhibition of the contaminant Lactobacillus fermentum from distilleries producing fuel bioethanol
Provedor de dados: 52
Autores: Oliva Neto,Pedro de; Lima,Fabíola Aliaga de; Silva,Ketrin Cristina da; Silva,Douglas Fernandes da; Carvalho,Ana Flavia Azevedo; Santos,Catarina dos.
The purpose of this study was to determine the Minimum Inhibitory Concentration (MIC) of pure or mixed chemicals for Saccharomyces cerevisiae and Lactobacillus fermentum in the samples isolated from distilleries with serious bacterial contamination problems. The biocides, which showed the best results were: 3,4,4' trichlorocarbanilide (TCC), tested at pH 4.0 (MIC = 3.12 mg/l), TCC with benzethonium chloride (CBe) at pH 6.0 (MIC = 3.12 mg/l) and TCC mixed with benzalkonium chloride (CBa) at pH 6.0 (MIC = 1.53 mg /l). If CBa was used in sugar cane milling in 1:1 ratio with TCC, a 8 times reduction of CBa was possible. This formulation also should be tested in fermentation steps since it was more difficult for the bacterium to develop resistance to biocide....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethanol; Lactic acid bacteria; Saccharomyces cerevisiae; Lactobacillus fermentum.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300019

Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin
Provedor de dados: 56
Autores: Bracesco,N.; Sosa,V.; Blanc,L.; Contreras,V.; Candreva,E.C.; Salvo,V.A.; Hocart,S.; Mechoso,B.; Nunes,E.
DNA repair pathways, cell cycle checkpoints, and redox protection systems are essential factors for securing genomic stability. The aim of the present study was to analyze the effect of Ilex paraguariensis (Ip) infusion and one of its polyphenolic components rutin on cellular and molecular damage induced by ionizing radiation. Ip is a beverage drank by most inhabitants of Argentina, Paraguay, Southern Brazil, and Uruguay. The yeast Saccharomyces cerevisiae (SC7Klys 2-3) was used as the eukaryotic model. Exponentially growing cells were exposed to gamma rays (γ) in the presence or absence of Ip or rutin. The concentrations used simulated those found in the habitual infusion. Surviving fractions, mutation frequency, and DNA double-strand breaks (DSB) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiation damage; Ilex paraguariensis infusion; Rutin; Radioprotection; Antimutagenic effects; Saccharomyces cerevisiae.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900606

The eukaryotic Pso2/Snm1/Artemis proteins and their function as genomic and cellular caretakers
Provedor de dados: 56
Autores: Bonatto,D.; Revers,L.F.; Brendel,M.; Henriques,J.A.P.
DNA double-strand breaks (DSBs) represent a major threat to the genomic stability of eukaryotic cells. DNA repair mechanisms such as non-homologous end joining (NHEJ) are responsible for the maintenance of eukaryotic genomes. Dysfunction of one or more of the many protein complexes that function in NHEJ can lead to sensitivity to DNA damaging agents, apoptosis, genomic instability, and severe combined immunodeficiency. One protein, Pso2p, was shown to participate in the repair of DSBs induced by DNA inter-strand cross-linking (ICL) agents such as cisplatin, nitrogen mustard or photo-activated bi-functional psoralens. The molecular function of Pso2p in DNA repair is unknown, but yeast and mammalian cell line mutants for PSO2 show the same cellular responses...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-homologous end joining; Double-strand breaks; V(D)J; PSO2; Artemis; Saccharomyces cerevisiae.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300002

Identification of the divergent calmodulin binding motif in yeast Ssb1/Hsp75 protein and in other HSP70 family members
Provedor de dados: 56
Autores: Heinen,R.C.; Diniz-Mendes,L.; Silva,J.T.; Paschoalin,V.M.F.
Yeast soluble proteins were fractionated by calmodulin-agarose affinity chromatography and the Ca2+/calmodulin-binding proteins were analyzed by SDS-PAGE. One prominent protein of 66 kDa was excised from the gel, digested with trypsin and the masses of the resultant fragments were determined by MALDI/MS. Twenty-one of 38 monoisotopic peptide masses obtained after tryptic digestion were matched to the heat shock protein Ssb1/Hsp75, covering 37% of its sequence. Computational analysis of the primary structure of Ssb1/Hsp75 identified a unique potential amphipathic alpha-helix in its N-terminal ATPase domain with features of target regions for Ca2+/calmodulin binding. This region, which shares 89% similarity to the experimentally determined calmodulin-binding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ssb1/Hsp75; Ca2+/calmodulin complex; Hsc70; Calmodulin-affinity peptide; Bag1; Saccharomyces cerevisiae.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100003

Photodynamic DNA damage induced by phycocyanin and its repair in Saccharomyces cerevisiae
Provedor de dados: 56
Autores: Pádula,M.; Boiteux,S.
In the present study, we analyzed DNA damage induced by phycocyanin (PHY) in the presence of visible light (VL) using a set of repair endonucleases purified from Escherichia coli. We demonstrated that the profile of DNA damage induced by PHY is clearly different from that induced by molecules that exert deleterious effects on DNA involving solely singlet oxygen as reactive species. Most of PHY-induced lesions are single strand breaks and, to a lesser extent, base oxidized sites, which are recognized by Nth, Nfo and Fpg enzymes. High pressure liquid chromatography coupled to electrochemical detection revealed that PHY photosensitization did not induce 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) at detectable levels. DNA repair after PHY...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phycocyanin; Saccharomyces cerevisiae; OGG1; Nucleotide excision repair.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900002

Evidence for a modulation of neutral trehalase activity by Ca2+ and cAMP signaling pathways in Saccharomyces cerevisiae
Provedor de dados: 56
Autores: Souza,A.C.; De Mesquita,J.F.; Panek,A.D.; Silva,J.T.; Paschoalin,V.M.F.
Saccharomyces cerevisiae neutral trehalase (encoded by NTH1) is regulated by cAMP-dependent protein kinase (PKA) and by an endogenous modulator protein. A yeast strain with knockouts of CMK1 and CMK2 genes (cmk1cmk2) and its isogenic control (CMK1CMK2) were used to investigate the role of CaM kinase II in the in vitro activation of neutral trehalase during growth on glucose. In the exponential growth phase, cmk1cmk2 cells exhibited basal trehalase activity and an activation ratio by PKA very similar to that found in CMK1CMK2 cells. At diauxie, even though both cells presented comparable basal trehalase activities, cmk1cmk2 cells showed reduced activation by PKA and lower total trehalase activity when compared to CMK1CMK2 cells. To determine if CaM kinase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Trehalase; Calmodulin; CaM kinase II; CAMP-dependent protein kinase; YLR270W.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100002

Glutathione peroxidase induction protects Saccharomyces cerevisiae sod1deltasod2delta double mutants against oxidative damage
Provedor de dados: 56
Autores: Manfredini,V.; Roehrs,R.; Peralba,M.C.R.; Henriques,J.A.P.; Saffi,J.; Ramos,A.L.L.P.; Benfato,M.S.
Saccharomyces cerevisiae mutants deficient in superoxide dismutase genes (sod1delta, sod2delta and the double mutant) were subjected to H2O2 stress in the stationary phase. The highest sensitivity was observed in the sod2delta mutant, while the sod1deltasod2delta double mutant was not sensitive. Sod mutants had lower catalase activity (44%) than wild-type cells, independent of H2O2 stress. Untreated cells of sod1deltasod2delta double mutants showed increased glutathione peroxidase activity (126%), while sod1delta had lower activity (77%) than the wild type. Glutathione levels in sod1delta were increased (200-260%) after exposure to various H2O2 concentrations. In addition, the highest malondialdehyde levels could be observed without H2O2 treatment in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catalase; Superoxide dismutase; Glutathione; Hydrogen peroxide; Saccharomyces cerevisiae; Reactive oxygen species.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200001

How does yeast respond to pressure?
Provedor de dados: 56
Autores: Fernandes,P.M.B.
The brewing and baking yeast Saccharomyces cerevisiae has been used as a model for stress response studies of eukaryotic cells. In this review we focus on the effect of high hydrostatic pressure (HHP) on S. cerevisiae. HHP exerts a broad effect on yeast cells characteristic of common stresses, mainly associated with protein alteration and lipid bilayer phase transition. Like most stresses, pressure induces cell cycle arrest. Below 50 MPa (500 atm) yeast cell morphology is unaffected whereas above 220 MPa wild-type cells are killed. S. cerevisiae cells can acquire barotolerance if they are pretreated with a sublethal stress due to temperature, ethanol, hydrogen peroxide, or pressure. Nevertheless, pressure only leads to protection against severe stress if,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Stress adaptation; Gene expression; High hydrostatic pressure.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800012

Pharmacology and toxicology of diphenyl diselenide in several biological models
Provedor de dados: 56
Autores: Rosa,R.M.; Roesler,R.; Braga,A.L.; Saffi,J.; Henriques,J.A.P.
The pharmacology of synthetic organoselenium compounds indicates that they can be used as antioxidants, enzyme inhibitors, neuroprotectors, anti-tumor and anti-infectious agents, and immunomodulators. In this review, we focus on the effects of diphenyl diselenide (DPDS) in various biological model organisms. DPDS possesses antioxidant activity, confirmed in several in vitro and in vivo systems, and thus has a protective effect against hepatic, renal and gastric injuries, in addition to its neuroprotective activity. The activity of the compound on the central nervous system has been studied since DPDS has lipophilic characteristics, increasing adenylyl cyclase activity and inhibiting glutamate and MK-801 binding to rat synaptic membranes. Systemic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diphenyl diselenide; Organoselenium; Antioxidants; Neuroprotection; Saccharomyces cerevisiae; Mutagenesis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000001

In vitro modulation of alkaline phosphatase activity of Saccharomyces cerevisiae grown in low or high phosphate medium
Provedor de dados: 56
Autores: Fernandes,J; Amorim,R; Azevedo,I; Martins,M.J.
Our objective was to characterize the modulation of the activity of Saccharomyces cerevisiae alkaline phosphatases (ALPs) by classic inhibitors of ALP activity, cholesterol and steroid hormones, in order to identify catalytic similarities between yeast and mammalian ALPs. S. cerevisiae expresses two ALPs, coded for by the PHO8 and PHO13 genes. The product of the PHO8 gene is repressible by Pi in the medium. ALP activity from yeast (grown in low or high phosphate medium) homogenates was determined with p-nitrophenylphosphate as substrate, pH 10.4 (lPiALP or hPiALP, respectively). Activation of hPiALP was observed with 5 mM L-amino acids (L-homoarginine _ 186%, L-leucine _ 155% and L-phenylalanine - 168%) and with 1 mM levamisole (122%; percentage values, in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Alkaline phosphatase inhibitors; PHO8 and PHO13; Cholesterol; Steroid hormones.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000100007

Study of a region on yeast chromosome XIII that complements pet G199 mutants (COX7) and carries a new non-essential gene
Provedor de dados: 56
Autores: Nobrega,M.P.; Graminha,M.A.S.; Troitskaya,E.N.; Nobrega,F.G.
The mutants of Saccharomyces cerevisiae assigned to complementation group G199 are deficient in mitochondrial respiration and lack a functional cytochrome oxidase complex. Recombinant plasmids capable of restoring respiration were cloned by transformation of mutants of this group with a yeast genomic library. Sequencing indicated that a 2.1-kb subclone encompasses the very end (last 11 amino acids) of the PET111 gene, the COX7 gene and a new gene (YMR255W) of unknown function that potentially codes for a polypeptide of 188 amino acids (about 21.5 kDa) without significant homology to any known protein. We have shown that the respiratory defect corresponding to group G199 is complemented by plasmids carrying only the COX7 gene. The gene YMR255W was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Gene cloning; Cytochrome oxidase; Subunit VII; Gene disruption.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300004

Probing the SERCA1a sarcoplasmic reticulum Ca2+-ATPase phosphorylation-site mutant D351E with inorganic phosphate
Provedor de dados: 56
Autores: Carreira,A.C.O.; Bastos,C.M.V.; Verjovski-Almeida,S.
The expression of sarcoplasmic reticulum SERCA1a Ca2+-ATPase wild-type and D351E mutants was optimized in yeast under the control of a galactose promoter. Fully active wild-type enzyme was recovered in yeast microsomal membrane fractions in sufficient amounts to permit a rapid and practical assay of ATP hydrolysis and phosphoenzyme formation from ATP or Pi. Mutant and wild-type Ca2+-ATPase were assayed for phosphorylation by Pi under conditions that are known to facilitate this reaction in the wild-type enzyme, including pH 6.0 or 7.0 at 25ºC in the presence of dimethylsulfoxide. Although glutamyl (E) and aspartyl (D) residue side chains differ by only one methylene group, no phosphoenzyme could be detected in the D351E mutant, even upon the addition of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phosphorylation site mutant; Catalytic site; Yeast expression system; Saccharomyces cerevisiae; P-type ATPase.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000004

"A comparison between sugar consumption and ethanol production in wort by immobilized Saccharomyces Cerevisiae, Saccharomyces Ludwigii and Saccharomyces Rouxii on Brewer's Spent Grain"
Provedor de dados: 58
Autores: Mohammadi,Aniseh; Razavi,Seyyed Hadi; Mousavi,Seyyed Mohammad; Rezaei,Karamatollah.
The immobilization of Saccharomyces cerevisiae DSM 70424, Saccharomyces ludwigii DSM 3447 and Saccharomyces rouxii DSM 2531 on brewer's spent grain and then ethanol production and sugar consumption of these immobilized yeasts were investigated. The aim of this study was to investigate the abilities of these three immobilized yeasts for producing alcohol for brewing at two temperatures (7 and 12 °C) using two different sugar levels (one at original level supplied in the brewery and one with 2.5% (w/v), added glucose to the wort). Increasing both parameters resulted in higher alcohol production by all the yeasts studied. At 7 °C and with original wort density the ethanol content at the end of fermentation was 2.7% (v/v) for S. cerevisiae, 1.7% for S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brewer's spent grain; Fermentation; Immobilization; Saccharomyces cerevisiae; Saccharomyces ludwigii; Saccharomyces rouxii.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200025

Double mutation of Saccharomyces cerevisiae for enhanced β-D-fructofuranosidase fructohydrolase productivity and application of growth kinetics for parametric significance analysis
Provedor de dados: 58
Autores: Ali,Sikander; Aslam,Aafia; Hayyat,Muhammad Umar.
Abstract The kinetics of an extracellular β-D-fructofuranosidase fructohydrolase production by Saccharomyces cerevisiae in a chemically defined medium, i.e., sucrose peptone agar yeast extract at pH 6, was investigated. The wild-type was treated with a chemical mutagen, methyl methane sulfonate. Among the six mutants isolated, methyl methane sulfonate-V was found to be a better enzyme producing strain (52 ± 2.4a U/mL). The maximum production (74 ± 3.1a U/mL) was accomplished after at 48 h (68 ± 2.7a mg/mL protein). The mutants were stabilized at low levels of 5-fluoro-cytocine and the viable ones were further processed for optimization of cultural conditions and nutritional requirements. The sucrose concentration, incubation period and pH were optimized to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Β-d-Fructofuranosidase fructohydrolase; Kinetics and thermodynamics; Entropy and enthalpy.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100136

Effects of pH and temperature on growth and glycerol production kinetics of two indigenous wine strains of Saccharomyces cerevisiae from Turkey
Provedor de dados: 58
Autores: Yalcin,Seda Karasu; Ozbas,Z. Yesim.
The study was performed in a batch system in order to determine the effects of pH and temperature on growth and glycerol production kinetics of two indigenous wine yeast strains Saccharomyces cerevisiae Kalecik 1 and Narince 3. The highest values of dry mass and specific growth rate were obtained at pH 4.00 for both of the strains. Maximum specific glycerol production rates were obtained at pH 5.92 and 6.27 for the strains Kalecik 1 and Narince 3, respectively. Kalecik 1 strain produced maximum 8.8 gL-1 of glycerol at pH 6.46. Maximum glycerol concentration obtained by the strain Narince 3 was 9.1 gL-1 at pH 6.48. Both yeasts reached maximum specific growth rate at 30ºC. Optimum temperature range for glycerol production was determined as 25-30ºC for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Production kinetics; Glycerol; Growth parameters; Saccharomyces cerevisiae.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200024

Production of bioethanol using agricultural waste: banana pseudo stem
Provedor de dados: 58
Autores: Ingale,Snehal; Joshi,Sanket J.; Gupte,Akshaya.
India is amongst the largest banana (Musa acuminata) producing countries and thus banana pseudo stem is commonly available agricultural waste to be used as lignocellulosic substrate. Present study focuses on exploitation of banana pseudo stem as a source for bioethanol production from the sugars released due to different chemical and biological pretreatments. Two fungal strains Aspergillus ellipticus and Aspergillus fumigatus reported to be producing cellulolytic enzymes on sugarcane bagasse were used under co-culture fermentation on banana pseudo stem to degrade holocellulose and facilitate maximum release of reducing sugars. The hydrolysate obtained after alkali and microbial treatments was fermented by Saccharomyces cerevisiae NCIM 3570 to produce...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioethanol; Banana pseudostem; Aspergillus ellipticus; Aspergillus fumigatus; Saccharomyces cerevisiae.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300018

Evaluation of stress tolerance and fermentative behavior of indigenous Saccharomyces cerevisiae
Provedor de dados: 58
Autores: Ramos,Cíntia Lacerda; Duarte,Whasley Ferreira; Freire,Ana Luiza; Dias,Disney Ribeiro; Eleutherio,Elis Cristina Araújo; Schwan,Rosane Freitas.
Sixty six indigenous Saccharomyces cerevisiae strains were evaluated in stressful conditions (temperature, osmolarity, sulphite and ethanol tolerance) and also ability to flocculate. Eighteen strains showed tolerant characteristics to these stressful conditions, growing at 42 ºC, in 0.04% sulphite, 1 mol L-1 NaCl and 12% ethanol. No flocculent characteristics were observed. These strains were evaluated according to their fermentative performance in sugar cane juice. The conversion factors of substrates into ethanol (Yp/s), glycerol (Yg/s) and acetic acid (Yac/s), were calculated. The highest values of Yp/s in sugar cane juice fermentation were obtained by four strains, one isolated from fruit (0.46) and the others from sugar cane (0.45, 0.44 and 0.43)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alcoholic fermentation; Biofuel; Fermentation kinetics; UFLA FW221; Saccharomyces cerevisiae.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300044

Partial purification of glucose-6-phosphate dehydrogenase by aqueous two-phase poly(ethyleneglycol)/phosphate systems
Provedor de dados: 58
Autores: Ribeiro,Marcela Zanella; Silva,Daniel Pereira; Vitolo,Michele; Roberto,Inês Conceição; Pessoa-Jr,Adalberto.
Glucose-6-phosphate dehydrogenase (G6PDH) is an important enzyme used in biochemical and medical studies and in several analytical methods that have industrial and commercial application. This work evaluated the extraction of G6PDH in aqueous two-phase system (ATPS) of poly(ethyleneglycol) (PEG)/phosphate buffer, using as enzyme source a medium prepared through commercial baker's yeast disruption. Firstly, the effects of PEG molar mass on the enzyme partition and of homogenization and rest on the system equilibrium were investigated. Afterwards, several ATPS were prepared using statistical analysis (2² factorial design). The results, including kinetic and thermodynamic parameters for the G6PDH activity, showed partial purification of this enzyme in ATPS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aqueous two-phase systems; Glucose-6-phosphate dehydrogenase; Liquid-liquid extraction; Saccharomyces cerevisiae.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100016

Trehalose accumulation, invertase activity and physiological characteristics of yeasts isolated from 24 h fermentative cycles during the production of artisanal Brazilian cachaça
Provedor de dados: 58
Autores: Pataro,Carla; Guerra,Juliana B.; Gomes,Fátima C.O.; Neves,Maria J.; Pimentel,Patrícia F.; Rosa,Carlos A.
Trehalose accumulation, invertase activity and physiological characteristics of 86 yeast isolates from short fermentative cycles during the production of cachaça in three artisanal distilleries of the State of Minas Gerais were studied. Among these isolates, 70% were able to grow at temperatures between 40 and 42ºC. Only Saccharomyces cerevisiae isolates were able to grow over 40ºC. Lower temperatures (&lt;40ºC) favoured the growth of yeasts such as Candida parapsilosis-like, C. maltosa-like, Kloeckera japonica, S. exiguus and C. bombicola-like. The isolates from all three distilleries were ethanol tolerant, produced invertase, and accumulate trehalose in the presence of glucose. The strains isolated from distillery A presented more resistance to ethanol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Cachaça; Trehalose; Invertase; Fermentation.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300003

Production and characterization of glucoamylase from fungus Aspergillus awamori expressed in yeast Saccharomyces cerevisiae using different carbon sources
Provedor de dados: 58
Autores: Pavezzi,Fabiana Carina; Gomes,Eleni; Silva,Roberto da.
Glucoamylase is widely used in the food industry to produce high glucose syrup, and also in fermentation processes for production beer and ethanol. In this work the productivity of the glucoamylase of Aspergillus awamori expressed by the yeast Saccharomyces cerevisiae, produced in submerged fermentation using different starches, was evaluated and characterized physico-chemically. The enzyme presented high specific activity, 13.8 U/mgprotein or 2.9 U/mgbiomass, after 48 h of fermentation using soluble starch as substrate. Glucoamylase presented optimum activity at temperature of 55ºC, and, in the substratum absence, the thermostability was for 1h at 50ºC. The optimum pH of activity was pH 3.5 - 4.0 and the pH stability between 5.0 and 7.0. The half life at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucoamylase; Aspergillus awamori; Saccharomyces cerevisiae; Corn starch; Potato starch; Cassava starch.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100024

The uptake of different iron salts by the yeast Saccharomyces cerevisiae
Provedor de dados: 58
Autores: Gaensly,Fernanda; Picheth,Geraldo; Brand,Debora; Bonfim,Tania M.B.
Yeasts can be enriched with microelements, including iron; however, special physicochemical conditions are required to formulate a culture media that promotes both yeast growth and iron uptake. Different iron sources do not affect biomass formation; however, considering efficacy, cost, stability, and compatibility with Saccharomyces cerevisiae metabolism, ferrous sulphate is recommended.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Saccharomyces cerevisiae; Iron uptake; Iron sources; Biomass formation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200016

Saccharomyces cerevisiae and non-Saccharomyces yeasts in grape varieties of the São Francisco Valley
Provedor de dados: 58
Autores: Ponzzes-Gomes,Camila M.P.B.S. de; Mélo,Dângelly L.F.M. de; Santana,Caroline A.; Pereira,Giuliano E.; Mendonça,Michelle O.C.; Gomes,Fátima C.O.; Oliveira,Evelyn S.; Barbosa Jr,Antonio M.; Trindade,Rita C.; Rosa,Carlos A.
The aims of this work was to characterise indigenous Saccharomyces cerevisiae strains in the naturally fermented juice of grape varieties Cabernet Sauvignon, Grenache, Tempranillo, Sauvignon Blanc and Verdejo used in the São Francisco River Valley, northeastern Brazil. In this study, 155 S. cerevisiae and 60 non-Saccharomyces yeasts were isolated and identified using physiological tests and sequencing of the D1/D2 domains of the large subunit of the rRNA gene. Among the non-Saccharomyces species, Rhodotorula mucilaginosa was the most common species, followed by Pichia kudriavzevii, Candida parapsilosis, Meyerozyma guilliermondii, Wickerhamomyces anomalus, Kloeckera apis, P. manshurica, C. orthopsilosis and C. zemplinina. The population counts of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian wines; Saccharomyces cerevisiae; Indigenous strains; Non-Saccharomyces; Mitochondrial DNA restriction analysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200007

Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress
Provedor de dados: 58
Autores: Sá,Rafael A. de; Castro,Frederico A.V. de; Eleutherio,Elis C.A.; Souza,Raquel M. de; Silva,Joaquim F.M. da; Pereira,Marcos D.
Propolis is a natural product widely used for humans. Due to its complex composition, a number of applications (antimicrobial, antiinflammatory, anesthetic, cytostatic and antioxidant) have been attributed to this substance. Using Saccharomyces cerevisiae as a eukaryotic model we investigated the mechanisms underlying the antioxidant effect of propolis from Guarapari against oxidative stress. Submitting a wild type (BY4741) and antioxidant deficient strains (ctt1∆, sod1∆, gsh1∆, gtt1∆ and gtt2∆) either to 15 mM menadione or to 2 mM hydrogen peroxide during 60 min, we observed that all strains, except the mutant sod1∆, acquired tolerance when previously treated with 25 µg/mL of alcoholic propolis extract. Such a treatment reduced the levels of ROS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propolis; Antioxidant; Oxidative stress; Saccharomyces cerevisiae.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300051

Effect of the trehalose levels on the screening of yeast as probiotic by in vivo and in vitro assays
Provedor de dados: 58
Autores: Martins,Flaviano S.; Miranda,Ieso C.; Rosa,Carlos A.; Nicoli,Jacques R.; Neves,Maria J.
Probiotics are viable defined microorganisms (bacteria or yeasts) that exert a beneficial effect on the health of the host when ingested in adequate amounts. Screening for such biotherapeutic agents is commonly performed by in vitro assays simulating gastrointestinal environment to determine the ability to survive in the digestive tract. In the present study, the possibility of extrapolation of data obtained in in vitro assays to in vivo conditions was studied using five Saccharomyces cerevisiae strains isolated from Brazilian Atlantic rain forest. Trehalose contents and survival after exposure to a combination of physiological stresses generally found in the gastrointestinal tract of humans were determined for the five yeasts and compared to the behavior...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Probiotic; Saccharomyces boulardii; Saccharomyces cerevisiae; Stress; Trehalose.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100012

Mobilization of endogenous glycogen and trehalose of industrial yeasts
Provedor de dados: 58
Autores: Paulillo,Silene Cristina de Lima; Yokoya,Fumio; Basso,Luiz Carlos.
The fermentation of yeast reserve carbohydrates, glycogen and trehalose is a procedure to increase protein level of yeast cells and improve ethanol production. This work studied on the degradation kinetics of glycogen and trehalose carried out with two industrial strains of Saccharomyces cerevisiae (PE-2 and SA-1) and the effect of different temperatures (38º, 40º, 42º and 44ºC) on degradation rate. Endogenous fermentation was carried out in a yeast suspension at 20% (w/v) based on wet weight, suspended in fermented media with 3.0 to 4.5 % (v/v) of ethanol. The degradation of the carbohydrate reserves at 40ºC followed first-order kinetics, showing that its rate is mainly dependent on the carbohydrate concentration in the cell. The degradation rate (k)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Glycogen; Trehalose; Yeast; Fermentation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300014

Filamentous growth in Saccharomyces cerevisiae
Provedor de dados: 58
Autores: Ceccato-Antonini,Sandra Regina; Sudbery,Peter Edwin.
Fungal dimorphism is a complex phenomenon triggered by a large variety of environmental factors and consists of a reversible alternating pattern of growth between different elliptical and filamentous forms of cells. Understanding the mechanisms that regulate these events is of major interest because of their implications in fungal pathogenesis, cell differentiation and industry. Diploid cells of Saccharomyces cerevisiae transform from budding yeast to pseudohyphae when starved for nitrogen, giving the cells an advantage in food foraging, which is sensed by at least two signal transduction pathways: the MAP kinase (MAPK) and the PKA (cAMP-dependent protein kinase A) pathways. The output of these signalling pathways is the expression of pseudohypha-specific...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Filamentation; Saccharomyces cerevisiae; Pseudohyphae.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200001

Electrical stimulation of saccharomyces cerevisiae cultures
Provedor de dados: 58
Autores: Araújo,Ofelia Q.F.; Coelho,Maria Alice Z.; Margarit,Isabel C.P.; Vaz-Junior,Carlos A.; Rocha-Leão,Maria Helena M.
Modulation of cell endogenous membrane potential by an external electrical field influences the structure and function of membrane compartments, proteins and lipid bi-layer. In this work, the effects of applied potential on Saccharomyces cerevisiae growth were characterized through simple yet conclusive experiments. Cell growth time profile and cell division were investigated as macroscopic response to the electrical stimulation. Control experiments were conducted under identical conditions except for the absence of applied potential. Through comparative analysis, electrical stimulation was verified to alter cell cycle as smaller sized population was observed, suggesting that a synchrony in cell division was promoted. Power spectral analysis was employed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electrochemical stress; Saccharomyces cerevisiae; Cell cycle; Viability.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100016

Factors involved with cadmium absorption by a wild-type strain of Saccharomyces cerevisiae
Provedor de dados: 58
Autores: Adamis,Paula D.B.; Panek,Anita D.; Leite,Selma G.F.; Eleutherio,Elis C.A.
At the concentration used in this work (10 ppm), cadmium was efficiently removed from the environment by stationary yeast cells. While exponential phase cells showed low capacity of cadmium absorption, stationary cells removed 97% of the original metal in 24 hours. Total cadmium absorption shown by dry cells was lower than that of fresh ones, although both cells removed 50% of metal during the first hour of treatment. We also verified that only viable cells were capable of absorbing cadmium. Independently of the growth phase, cells showed high tolerance to 10 ppm CdSO4 and about 80% of cells remained viable after 24 hours exposure to cadmium. However, when stationary phase cells were previously dehydrated and then exposed to cadmium, they exhibited poor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Cadmium; Absorption; Intracellular oxidation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100012

Construction of killer industrial yeast Saccharomyces cerevisiae HAU-1 and its fermentation performance
Provedor de dados: 58
Autores: Bajaj,Bijender K.; Sharma,S.
Saccharomyces cerevisiae HAU-1, a time tested industrial yeast possesses most of the desirable fermentation characteristics like fast growth and fermentation rate, osmotolerance, high ethanol tolerance, ability to ferment molasses, and to ferment at elevated temperatures etc. However, this yeast was found to be sensitive against the killer strains of Saccharomyces cerevisiae. In the present study, killer trait was introduced into Saccharomyces cerevisiae HAU-1 by protoplast fusion with Saccharomyces cerevisiae MTCC 475, a killer strain. The resultant fusants were characterized for desirable fermentation characteristics. All the technologically important characteristics of distillery yeast Saccharomyces cerevisiae HAU-1 were retained in the fusants, and in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Killer yeast; Distillery yeast; Saccharomyces cerevisiae; Molasses; Ethanol fermentation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200030

Asparaginase II-GFP fusion as a tool for studying the secretion of the enzyme under nitrogen starvation
Provedor de dados: 58
Autores: Sotero-Martins,Adriana; Bon,Elba Pinto da Silva; Carvajal,Elvira.
Production of asparaginase II of Saccharomyces cerevisiae is regulated by nitrogen and can be used as a model system for studying other secreted proteins in yeast. Green fluorescent protein (GFP) from Aequorea victoria was fused to the carboxy-terminus of the enzyme by genomic integration to the locus ASP3 of S. cerevisiae. We determined asparaginase II activity, mRNA ASP3, mRNA ASP3-GFP and GFP fluorescence. Nitrogen starvation in cells carrying the chimera ASP3-GFP caused an increase in fluorescence and in the expression of ASP3. We have shown that cells producing the chimera Asp3-GFPp displayed the same response to nitrogen starvation as control cells. We demonstrated that Asp3-GFPp can be used for studying asparaginase II secretion under nitrogen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asparaginase II; Nitrogen regulation; Saccharomyces cerevisiae; Genomic integration; Green fluorescent protein.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400017

Saccharomyces cerevisiae populations and other yeasts associated with indigenous beers (chicha) of Ecuador
Provedor de dados: 58
Autores: Piló,Fernanda Barbosa; Carvajal-Barriga,Enrique Javier; Guamán-Burneo,Maria Cristina; Portero-Barahona,Patricia; Dias,Arthur Matoso Morato; Freitas,Larissa Falabella Daher de; Gomes,Fátima de Cássia Oliveira; Rosa,Carlos Augusto.
ABSTRACT Chicha, a type of beer made mainly with maize or cassava, is a traditional fermented beverage of the Andean region. There have only been a few studies on yeasts associated with chicha fermentation, and the species diversity occurring during the production of this beverage is not known. The objective of this study was to determine the biodiversity of yeasts in chicha, and to characterize the Saccharomyces cerevisiae populations associated with the production of chicha de jora, seven-grain chicha, chicha de yuca, and chicha de morocho in Ecuador. The molecular diversity of S. cerevisiae populations was determined by restriction polymorphism mitochondrial profiles. The beverages were characterized based on their physicochemical parameters. Twenty-six...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; MtDNA restriction patterns; Yeast populations; Chicha; Ecuadorian indigenous beers.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400808

Ethanol production from sweet sorghum by Saccharomyces cerevisiae DBKKUY-53 immobilized on alginate-loofah matrices
Provedor de dados: 58
Autores: Nuanpeng,Sunan; Thanonkeo,Sudarat; Klanrit,Preekamol; Thanonkeo,Pornthap.
Abstract Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM) was successfully developed. As found in this study, an ALM with dimensions of 20 × 20 × 5 mm3 is effective for cell immobilization due to its compact structure and long-term stability. The ALM-immobilized cell system exhibited greater ethanol production efficiency than the freely suspended cell system. By using a central composite design (CCD), the optimum conditions for ethanol production from SSJ by ALM-immobilized cells were determined. The maximum ethanol concentration and volumetric ethanol productivity obtained using ALM-immobilized cells under the optimal conditions were 97.54...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alginate-loofah-matrix; Ethanol production; Saccharomyces cerevisiae; Sweet sorghum; Thermotolerant yeast.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500140

Monitoring Saccharomyces cerevisiae populations by mtDNA restriction analysis and other molecular typing methods during spontaneous fermentation for production of the artisanal cachaça
Provedor de dados: 58
Autores: Araújo,Roberta A.C.; Gomes,Fátima C.O.; Moreira,Elizabeth S.A.; Cisalpino,Patrícia S.; Rosa,Carlos A.
An ecological study on Saccharomyces cerevisiae populations in spontaneous fermentation has been conducted in three vats of a cachaça distillery in Minas Gerais, Brazil. Ninety-seven yeast isolates were collected at the beginning, the middle and at the end of the production period, and were identified by standard methods. Differentiation between the indigenous S. cerevisiae strains isolated was performed by mitochondrial DNA (mtDNA) restriction analysis, RAPD-PCR, and PCR fingerprint using an intron splice primer. Analysis of the mtDNA restriction profiles revealed 12 different patterns, 11 corresponding to indigenous yeasts (I to XI) and one (XII) to a commercial strain of the bakery yeast. Pattern II (53.6% of the population) and pattern IV strains were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Cachaça; Fermentation; Molecular diversity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200006

Yeast biomass production: a new approach in glucose-limited feeding strategy
Provedor de dados: 58
Autores: Vieira,Érika Durão; Andrietta,Maria da Graça Stupiello; Andrietta,Silvio Roberto.
The aim of this work was to implement experimentally a simple glucose-limited feeding strategy for yeast biomass production in a bubble column reactor based on a spreadsheet simulator suitable for industrial application. In biomass production process using Saccharomyces cerevisiae strains, one of the constraints is the strong tendency of these species to metabolize sugars anaerobically due to catabolite repression, leading to low values of biomass yield on substrate. The usual strategy to control this metabolic tendency is the use of a fed-batch process in which where the sugar source is fed incrementally and total sugar concentration in broth is maintained below a determined value. The simulator presented in this work was developed to control molasses...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Biomass; Fed-batch; Process simulation; Saccharomyces cerevisiae.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200035

Characterization of the Saccharomyces cerevisae Y500-4L killer toxin
Provedor de dados: 58
Autores: Soares,Giselle A. M.; Sato,Hélia H.
The strain Saccharomyces cerevisiae Y500-4L, selected from the must of alcohol producing plants, liberates a toxin which is lethal to the commercial yeast produced by Fleischmann Royal Nabisco and other strains of yeast. This toxin was characterized, and the maximum production was obtained after 24 hours of incubation at 25ºC in YEPD medium. The maximum activity was achieved between pH 4.1 and 4.5 and between 22 and 25ºC and maximum stability in the pH range 3.8 to 4.5 at -10ºC. The killer toxin was inactivated by heating at 40ºC for 1 hour at pH 4.1. After concentration by ultrafiltration of culture supernatants and purification by gel filtration chromatography, the molecular weight of the purified toxin was estimated by SDS-PAGE to be about 18-20 kDa.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Killer toxin; Saccharomyces cerevisiae; Killer yeast.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400010

Evaluation of cytochrome P-450 concentration in Saccharomyces cerevisiae strains
Provedor de dados: 60
Autores: Matuo,Míriam Cristina Sakuragui; Kikuchi,Irene Satiko; Pinto,Terezinha de Jesus Andreoli.
Saccharomyces cerevisiae has been widely used in mutagenicity tests due to the presence of a cytochrome P-450 system, capable of metabolizing promutagens to active mutagens. There are a large number of S. cerevisiae strains with varying abilities to produce cytochrome P-450. However, strain selection and ideal cultivation conditions are not well defined. We compared cytochrome P-450 levels in four different S. cerevisiae strains and evaluated the cultivation conditions necessary to obtain the highest levels. The amount of cytochrome P-450 produced by each strain varied, as did the incubation time needed to reach the maximum level. The highest cytochrome P-450 concentrations were found in media containing fermentable sugars. The NCYC 240 strain produced the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Cytochrome P-450/obtention; Cytochrome P-450/cultivation conditions; Ethanol; Carbon sources.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300011

Effect of the type of substrate on the chemical composition and productivity of a protein concentrate of yeast origin
Provedor de dados: 118
Autores: Velásquez,Alejandro; Arias,Rodrigo; Toneatti,Marcelo.
Extracts of Saccharomyces cerevisiae (Sc) yeast were generated using a biotechnological solid-state fermentation method. The organic material used for fermentation consisted of a variety of fibrous substrates of agricultural origin, and their effect on the chemical composition and productivity of cellular biomass generated was evaluated. The substrates studied included the following: wheat straw, WS; barley straw, BS; chili stubble, CS; oat hull, OH and starch powder, SP (control). For incubation with Sc, 2 g of (dry) vegetable substrate were added to 12 mL of cultivation medium. The fermentation time was seven days under aerobic conditions (pH 5, 26°C). After extraction of the yeast biomass, its chemical composition and productivity were analyzed. The...
Tipo: Journal article
Palavras-chave: Protein concentrate; Saccharomyces cerevisiae; Vegetable substrate.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300003

Effect of substrate on the in vitro protein digestibility of extracts generated by Saccharomyces cerevisiae
Provedor de dados: 118
Autores: Velásquez,Alejandro; Arias,Rodrigo.
A. Velásquez, and R. Arias. 2013. Effect of substrate on the in vitro protein digestibility of extracts generated by Saccharomyces cerevisiae. Cien. Inv. Agr. 40(3): 503-511. The in vitro crude protein (CP) digestibility (Divcp), true protein digestibility (Dtp) and degradation rate (kd cp) were measured in different protein extracts of Saccharomyces cerevisiae (Sc) yeast. These extracts were generated through a biotechnological method (solid state fermentation). Wheat straw (TWS), barley straw (TBS), chili stubble (TCS), oats hull (TOH) and starch-glucose powder (TSP; control) were used as substrates for fermentation by Sc, and their effect on the aforementioned kinetic parameters in the generated cellular biomass was evaluated. In 12 mL cultivation...
Tipo: Journal article
Palavras-chave: In vitro protein digestibility; Protein extract; Saccharomyces cerevisiae; Vegetable substrates.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000300004

Utilização de levedura íntegra e seus derivados em dietas para juvenis de tilápia do Nilo
Provedor de dados: 65
Autores: Gonçalves,Ligia Uribe; Carvalho,Márcia de; Viegas,Elisabete Maria Macedo.
Neste trabalho, avaliou-se a adição de células íntegras de levedura e seus derivados em dietas para juvenis de tilápia do Nilo. Foram utilizados 144 juvenis machos de tilápia (peso médio de 52,1g) distribuídos em 12 tanques de fibra de vidro (250L), em delineamento inteiramente casualizado, composto por quatro tratamentos e três repetições. Os peixes foram alimentados ad libitum, duas vezes ao dia durante 60 dias, com dietas isoproteicas (28% PB) e isocalóricas (2.900kcal de ED kg-1) contendo levedura íntegra de cana-de-açúcar (LI), levedura autolisada (LA) e parede celular (PC) adicionados na proporção de 25% da proteína bruta total, comparadas com uma dieta controle (CO), sem adição de levedura. Não foram observadas diferenças significativas para...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oreochromis niloticus; Levedura de cana-de-açúcar; Desempenho zootécnico; Parede celular; Levedura autolisada; Saccharomyces cerevisiae.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000500027