Consulta: subjectFacets:"Salmonella"
Registros recuperados: 152
Data/hora: 13/06/2024 04:59:42

Atmósferas modificadas en modalidad de microperforado, enterobacterias y residuos de plaguicidas en nopal verdura (Opuntia ficus indica L.).
Provedor de dados: 32
Autores: Meraz Maldonado, Nora.
El nopal verdura es una hortaliza que aporta cantidades importantes de nutrimentos a la dieta en México. Sin embargo, cuando éste es usado como producto mínimamente procesado, la vida de anaquel se limita a los cambios en coloración de la piel y heridas pero, también debe ser considerada, dentro de la calidad, la inocuidad relacionada a la contaminación química y microbiológica. El presente estudio aborda éstos aspectos en seis capítulos que tienen por objetivos: 1) Determinar la predilección del consumidor en la adquisición de nopal verdura por tamaño, color, presentación, acidez y fibra. 2) Evaluar algunos cambios en la calidad por efecto del tamaño en nopal verdura entero desespinado. 3) Determinar el efecto de las atmósferas modificadas en la calidad...
Palavras-chave: Opuntia ficus-indica; Atmósferas modificadas; Salmonella; Escherichia coli; Malatión; Clorpirifos etílico; Entomología y Acarología; Doctorado; Modified atmospheres; Malathion; Chlorpyrifos ethyl.
Ano: 2012
URL: http://hdl.handle.net/10521/1819

Calidad sanitaria en la producción de nopal verdura (Opuntia sp.) en Otumba, México.
Provedor de dados: 32
Autores: Martínez Martínez, Talina Olivia.
La calidad sanitaria en los productos hortofrutícolas debe considerarse una prioridad en la producción de alimentos. Actualmente en México, existe una gran preocupación por las asociaciones de productores y gobierno federal para adoptar sistemas de producción que reduzcan riesgos de contaminación (SRRC). Para la implementación de estos sistemas, es necesario cumplir con una serie de requisitos, entre ellos se encuentra la validación de procesos. Un método inmediato para efectuar este requerimiento, es la evaluación de la calidad sanitaria desde el punto de vista microbiológico y toxicológico, lo que hace necesario determinar un plan de muestreo y las técnicas apropiadas para la detección y cuantificación de patógenos indicadores. Con este propósito, en...
Palavras-chave: Salmonella; Escherichia coli; Pesticides; Phenolic compounds; Survival; Sample size; Entomología y Acarología; Doctorado.
Ano: 2011
URL: http://hdl.handle.net/10521/590

Formación de biopelículas y resistencia a desinfectantes en cepas de salmonella aisladas de nopal verdura y muestras de agua y suelo de uso agrícola.
Provedor de dados: 32
Autores: Santos Villamil, Alfricia Adriana de los.
La presencia de Salmonella en nopal verdura fresco (Opuntia ficus-indica L.) MILL se ha documentado recientemente (Hernández et al., 2009). La contaminación de alimentos es un problema de salud pública que afecta a las personas en todo el mundo. Una característica importante de Salmonella es su capacidad para formar biopelículas, las cuales son comunidades de células microbianas embebidas en una matriz de exopolímeros que ellas mismas producen en respuesta a factores como la temperatura, la humedad relativa y la composición del medio en que se encuentran (Annous et al., 2005; Stepanovic et al., 2000). Esta capacidad le permite a Salmonella adherirse de manera irreversible y de colonizar superficies bióticas y abióticas (Annous et al., 2005, Stepanovic et...
Palavras-chave: Biopelículas; Desinfectantes; Nopal; Salmonella; Biofilms; Desinfectants; Maestría; Fitopatología.
Ano: 2010
URL: http://hdl.handle.net/10521/220

Actividad antimicrobiana y caracterización fenólica de extractos de cálices de jamaica (Hibiscus sabdariffa L.).
Provedor de dados: 32
Autores: Morales Cabrera, Mercedes.
En este estudio se evaluó la actividad antimicrobiana de los extractos etanólicos, metanólicos y acuosos (frío y caliente) de cálices deshidratados de Hibiscus sabdariffa L. de cinco genotipos, sobre S. typhimurium (ATCC14028) y S. cholleraesuis (ATCC10708), se empleó el método difusión en agar. Se determinó la concentración mínima inhibitoria (CMI) y la concentración mínima bactericida (CMB) por dilución. Se analizó la influencia del pH en la actividad antimicrobiana. Así mismo se realizó la caracterización fenólica de los extractos etanólicos. El efecto antimicrobiano fue variable; los extractos de la variedad Alma blanca (cálices verdes), presentaron el mayor efecto antimicrobiano (diámetros de inhibición de12.5 a 19.5 mm) sobre ambos serotipos de...
Palavras-chave: CMI; CMB; PH; Salmonella; Compuestos fenólicos; HPLC; MIC; MCB; Phenolic compounds; Fitopatología; Maestría.
Ano: 2011
URL: http://hdl.handle.net/10521/568

Calidad sanitaria de jugos de nopal, persistencia de Salmonella typhimurium y respuesta de defensa de nopal verdura.
Provedor de dados: 32
Autores: Landa Salgado, Patricia.
Durante las últimas dos décadas las infecciones por Salmonella se han registrado entre las enfermedades más frecuentes, afectando a personas de todas las edades y condiciones sociales y causando numerosas hospitalizaciones y muerte de personas por el consumo de frutas y hortalizas frescas. En frutas, en 2011 el Centro para el Control de Enfermedades y Prevención en EUA reportó un brote de infección por S. Agona asociado con el consumo de papaya fresca en el que 106 personas resultaron afectadas. Mientras que en hortalizas, S. typhimurium se ha aislado de diversas especies entre las que se encuentra el nopal verdura (Opuntia ficus-indica L. Mill) (Quiroz-Santiago, 2009; Hernández et. al. 2009). Debido a que el nopal se consume en fresco para el control de...
Palavras-chave: Salmonella; Nopal; Persistencia; Respuesta de defensa nopal; Persistence; Defense of nopal; Doctorado; Fitopatología.
Ano: 2012
URL: http://hdl.handle.net/10521/745

Actividad antimicrobiana y caracterización fenólica de extractos de cálices de jamaica (Hibiscus sabdariffa L.).
Provedor de dados: 32
Autores: Morales Cabrera, Mercedes.
En este estudio se evaluó la actividad antimicrobiana de los extractos etanólicos, metanólicos y acuosos (frío y caliente) de cálices deshidratados de Hibiscus sabdariffa L. de cinco genotipos, sobre S. typhimurium (ATCC14028) y S. cholleraesuis (ATCC10708), se empleó el método difusión en agar. Se determinó la concentración mínima inhibitoria (CMI) y la concentración mínima bactericida (CMB) por dilución. Se analizó la influencia del pH en la actividad antimicrobiana. Así mismo se realizó la caracterización fenólica de los extractos etanólicos. El efecto antimicrobiano fue variable; los extractos de la variedad Alma blanca (cálices verdes), presentaron el mayor efecto antimicrobiano (diámetros de inhibición de12.5 a 19.5 mm) sobre ambos serotipos de...
Palavras-chave: CMI; CMB; PH; Salmonella; Compuestos fenólicos; HPLC; MIC; MCB; Phenolic compounds; Fitopatología; Maestría.
Ano: 2011
URL: http://hdl.handle.net/10521/568

Formación de biopelículas y resistencia a desinfectantes en cepas de salmonella aisladas de nopal verdura y muestras de agua y suelo de uso agrícola.
Provedor de dados: 32
Autores: Santos Villamil, Alfricia Adriana de los.
La presencia de Salmonella en nopal verdura fresco (Opuntia ficus-indica L.) MILL se ha documentado recientemente (Hernández et al., 2009). La contaminación de alimentos es un problema de salud pública que afecta a las personas en todo el mundo. Una característica importante de Salmonella es su capacidad para formar biopelículas, las cuales son comunidades de células microbianas embebidas en una matriz de exopolímeros que ellas mismas producen en respuesta a factores como la temperatura, la humedad relativa y la composición del medio en que se encuentran (Annous et al., 2005; Stepanovic et al., 2000). Esta capacidad le permite a Salmonella adherirse de manera irreversible y de colonizar superficies bióticas y abióticas (Annous et al., 2005, Stepanovic et...
Palavras-chave: Biopelículas; Desinfectantes; Nopal; Salmonella; Biofilms; Desinfectants; Maestría; Fitopatología.
Ano: 2010
URL: http://hdl.handle.net/10521/220

Phage-resistance of Salmonella enterica serovar Enteritidis and pathogenesis in Caenorhabditis elegans is mediated by the lipopolysaccharide
Provedor de dados: 69
Autores: Santander,Javier; Robeson,James.
Phage therapy has been used in the past as an alternative therapy against bacterial pathogens. However, phage-resistant bacterial strains can emerge. Some studies show that these phage-resistant strains are avirulent. In this study, we report that phage-resistant strains of Salmonella enterica serovar Enteritidis (hereafter S. Enteritidis) were avirulent in the Caenorhabditis elegans animal model. We isolated phage-resistant strains of S. Enteritidis ATCC 13076 by using three lytic phages (f2&amp;#945;SE, f3&amp;#945;SE and f18&amp;#945;SE). In these mutants, we explored different virulence factors like lipopolysaccharide (LPS), virulence plasmid (Pla), motility and type I fimbriae, all of which may have effects on virulence and could furthermore be...
Tipo: Journal article
Palavras-chave: Animal model; Bacteriophage prophylaxis; Caenorhabditis elegans; Phage resistance; Salmonella.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400015

Stability inside hen eggs of a Salmonella enterica serovar Enteritidis bacteriophage
Provedor de dados: 69
Autores: Robeson,James; Valencia,Martina; Retamales,Julio; Borie,Consuelo.
Background: Bacteriophages are viruses that infect bacteria and therefore are widespread in nature. Those that lyse the pathogen Salmonella enterica serovar Enteritidis (SE) should be expected in niches in which this bacterium thrives, among others the avian egg. Furthermore, bacteriophages could remain viable in this milieu. Results: Upon artificially infecting hen eggs with the SE bacteriophage f18 we found that the bacteriophage titer remains stable at least for up to 144 hrs post-infection , both in yolk and albumen at 25ºC. Conclusion: Bacteriophage f18 withstands the physico-chemical conditions of the egg inner milieu and could be considered for SE-controlling measures in the poultry industry.
Tipo: Journal article
Palavras-chave: Bacteriophage; Egg; Enteritidis; Salmonella.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400011

Inhibition of NF-kB 1 (NF-kBp50) by RNA interference in chicken macrophage HD11 cell line challenged with Salmonella enteritidis
Provedor de dados: 74
Autores: Chiang,Hsin-I; Berghman,Luc R.; Zhou,Huaijun.
The NF-kB pathway plays an important role in regulating the immunity response in animals. In this study, small interfering RNAs (siRNA) were used to specifically inhibit NF-kB 1 expression and to elucidate the role of NF-kB in the signal transduction pathway of the Salmonella challenge in the chicken HD11 cell line. The cells were transfected with either NF-kB 1 siRNA, glyceraldehyde 3-phosphate dehydrogenase siRNA (positive control) or the negative control siRNA for 24 h, followed by Salmonella enteritidis (SE) challenge or non-challenge for 1 h and 4 h. Eight candidate genes related to the signal pathway of SE challenge were selected to examine the effect of NF-kB 1 inhibition on their expressions by mRNA quantification. The results showed that, with a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNA interference; NF-kBp50; Macrophage; Salmonella; Chicken.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300013

Qualidade microbiológica de tomates provenientes da produção orgânica e convencional.
Provedor de dados: 14
Autores: PENTEADO, A. L.
bitstream/item/83105/1/2012-306.pdf
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: E coli.; Salmonella; Tomate.
Ano: 2012
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/958420

Detecção de Salmonella spp. a partir de carcaças de bovinos obtidas durante o processamento em abatedouros-frigoríficos em Mato Grosso do Sul: resultados preliminares
Provedor de dados: 14
Autores: ARAUJO, F. R.; BIER, D.; VERBISCK, N. V.; RAMOS, C. L. do N.; RODRIGUES, D. dos P.; VALSONI, L. M.; KICH, J. D.; DUARTE, S. C.
bitstream/item/158118/1/Deteccao-de-Salmonella-spp.-a-partir-de-carcacas.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Abate; Bovino; Carcaça; Frigorífico; Salmonella.
Ano: 2015
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1045832

Fontes importantes de contaminação por Salmonella em granjas terminadoras de suínos.
Provedor de dados: 14
Autores: KICH, J. D.; BORDIN, L. C.; PRATES, A.; MORES, N.; COLDEBELLA, A.; TRIQUES, N.; KLEIN, E. L.; RAMENZONI, M. L. F.; SILVA, L.
bitstream/item/86001/1/DCOT-389.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Suínos.; Contaminação; Salmonella; Terminação.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/962063

Contribuições na prevenção da resistência aos antimicrobianos: bacteriófagos para controle de bactérias na avicultura. Concórdia: Embrapa Suínos e Aves, 2022, 16 p.
Provedor de dados: 14
Autores: VAZ, C. S. L.; RECH, D. V.; FONSECA, F. M. da.
Tipo: Folhetos
Palavras-chave: Antimicrobial resistance; Bacteriófagos; Resistência antimicrobiana; Fagoterapia; Bacteria in poultry; Biological inputs; Phagotherapy; Poultry farming; Bacterium; Avicultura; Salmonella; Bactéria; Bacteriophages; Aviculture.
Ano: 2022
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1147505

Mecanismos de invasão do ovo por Salmonela sp.
Provedor de dados: 14
Autores: DUARTE, S. C.; RAUECKER, U. N.; REZENDE, C. S. M.
Com o intuito de gerar melhor compreensão da dinâmica de infecção por Salmonella sp. no trato reprodutor de poedeiras reunimos algumas informações acerca dos principais mecanismos envolvidos na persistência da bactéria no trato reprodutivo de poedeiras e os elementos envolvidos na contaminação de ovos.
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Poedeira; Produção de ovos; Contaminação de ovos; Reprodução animal; Galinha para postura; Salmonella; Egg production.
Ano: 2017
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1069583

O uso da sorologia (ELISA) para monitorar a infecção por Salmonella em rebanhos suínos.
Provedor de dados: 14
Autores: KICH, J. D.; SCHWARZ, P.; CARDOSO, M.; TRIQUES, N.; VIZZOTTO, R.; RAMENZONI, M. L. F.
bitstream/CNPSA/15910/1/publicacao_b0d5u2t.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Teste de Elisa.; Salmonella; Suíno.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/443782

Compreendendo os mecanismos de invasão da Salmonella nas aves e a resposta imune.
Provedor de dados: 14
Autores: DUARTE, S. C.; BASTOS, A. P. A.
As aves podem ser infectadas com salmonella a qualquer momento durante a sua vida. Contudo as infecções que ocorrem dentro das primeiras horas ou dias de vida são mais frequentes, pois neste período estes animai são altamente sensíveis à bactéria.
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Avicultura; Sanidade animal; Bacteriologia; Salmonella.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1061710

Por que os ovos não são contaminados por Salmonela?
Provedor de dados: 14
Autores: DUARTE, S. C.; WALTER, E. H. M.
bitstream/item/216817/1/final9438.pdf
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Produção de ovos; Qualidade do ovo; Alimento seguro; Controle Microbiano; Microrganismo; Salmonella; Contaminação Bacteriana; Segurança Alimentar.
Ano: 2020
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1125661

Qualidade microbiológica de alface fertirrigada por gotejamento com fertilizantes orgânicos ou mineral.
Provedor de dados: 14
Autores: PILON, L.; GINANI, V. C.; FONTENELLE, M. R.; LIMA, C. E. P.; BRAGA, M. B.; ZANDONADI, D. B.
O objetivo desse trabalho foi comparar a qualidade microbiológica de alfaces 'Laurel' produzidas em cultivo protegido, fertilirrigadas por gotejamento com biofertilizante Horbio®, biofertilizante comercial e fertilizante mineral, bem como a qualidade microbiológica desses fertilizantes.
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Hortbio; Lactuca Sativa; Biofertilizante; Salmonella.
Ano: 2019
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1109618

Controle de salmonela em suínos: situação atual e estratégias baseadas na relação hospedeiro-patógeno.
Provedor de dados: 14
Autores: KICH, J. D.; CARDOSO, M. R. de I.
bitstream/item/92812/1/7041.pdf
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Animal host; Hospedeiro animal; Suíno; Salmonella; Produção animal; Swine; Animal production; Slaughter.
Ano: 2013
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/971709

Detecção de Salmonella sp. em leite utilizando biossensor eletroquímico.
Provedor de dados: 14
Autores: MELO, A. M. A.; ALEXANDRE, D. L.; FURTADO, R. F.; BORGES, M. de F.; ALVES, C. R.; FIGUEIREDO, E. A. T. de.
bitstream/item/161503/1/COT16008.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Metodologia; Performance biossensor.; Salmonella; Leite.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1054785

Salmonella em suínos: alternativas de controle.
Provedor de dados: 14
Autores: SCHWARZ, P.; KICH, J. D.; CARDOSO, M.
bitstream/item/43002/1/Jalusa-Avicultura0001.pdf
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Sanidade.; Salmonella; Suíno.
Ano: 2011
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/902677

Presence of Salmonella in shellfish in the Thau lagoon
Provedor de dados: 5
Autores: Angeli, J.
After a general presentation of the Thau lagoon together with its geographic location, socio-economic activities linked to shellfish culture and fishing, this paper investigates the health conditions of the shellfish harvesting areas. I he influence of heavy rainfall following a prolonged drought period is underlined as a determining factor of the momentary bacterial pollution in the harvesting areas, leading to a suspension of sales based on analyses relying solely on the detection of faecal coliforms. As of 1989, bacteriological monitoring was reorganized in the form of a data collection network taking into consideration environmental assessments of water quality as well as shellfish hygiene in order to protect consumer health. Its extension to the...
Tipo: Text
Palavras-chave: Salmonella; Bivalvia; Surveillance and enforcement; Mollusc culture; Health and safety; Epidemiology.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/1992/acte-1589.pdf

Survie et maintien de la virulence de Salmonella Typhimurium VNC exposée simultanément à trois facteurs stressants expérimentaux
Provedor de dados: 5
Autores: Baleux, B; Caro, A; Lesne, J; Got, P; Binard, S; Delpeuch, B.
The release of enteric pathogenic bacteria in aquatic environments poses the problem of the fate of these bacteria under the effects of environmental factors (solar radiation, salt concentration, temperature, nutrient level, pH, competition). Frequently, these bacterial cells, potentially pathogenic, enter into a non-culturable state on routine bacteriological plating media. However, the use of direct detection methods (DAPI stained cells) allows the visualization of these Viable but Non Culturable cells (VNC). But, beyond the characterization of the viability of the cells (electron transport activity, metabolic activity, membrane integrity, structure and/or quantity of DNA), what happens with the virulence of these cells? This problem was experimentally...
Tipo: Text
Palavras-chave: Salmonella; Survie; Virulence; VNC; Stress; Salmonella; Survival; Virulence; VNC; Stress.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00325/43645/43245.pdf

Occurrence of Bacterial Pathogens and Human Noroviruses in Shellfish-Harvesting Areas and Their Catchments in France
Provedor de dados: 5
Autores: Rince, Alain; Baliere, Charlotte; Hervio-heath, Dominique; Cozien, Joelle; Lozach, Solen; Parnaudeau, Sylvain; Le Guyader, Francoise S.; Le Hello, Simon; Giard, Jean-christophe; Sauvageot, Nicolas; Benachour, Abdellah; Strubbia, Sofia; Gourmelon, Michele.
During a 2-year study, the presence of human pathogenic bacteria and noroviruses was investigated in shellfish, seawater and/or surface sediments collected from three French coastal shellfish-harvesting areas as well as in freshwaters from the corresponding upstream catchments. Bacteria isolated from these samples were further analyzed. Escherichia coli isolates classified into the phylogenetic groups B2, or D and enterococci from Enterococcus faecalis and E. faecium species were tested for the presence of virulence genes and for antimicrobial susceptibility. Salmonella members were serotyped and the most abundant serovars (Typhimurium and its monophasic variants and Mbandaka) were genetically characterized by high discriminative subtyping methods....
Tipo: Text
Palavras-chave: Campylobacter; Salmonella; Vibrio; HuNoVs; Fecal bacterial indicators; Shellfish; Water.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00461/57243/59243.pdf

Effects of propolis from Brazil and Bulgaria on Salmonella serovars
Provedor de dados: 80
Autores: Orsi,R. O.; Sforcin,J. M.; Funari,S. R. C.; Fernandes-JR.,A.; Rodrigues,P.; Bankova,V.
Propolis shows biological properties such as antibacterial action. This bee product has a complex chemical composition, which depends on the local flora where it is produced. Salmonella serovars are responsible for human diseases that range from localized gastroenteritis to systemic infections. The aim of the present study was to investigate the susceptibility of Salmonella strains, isolated from food and infectious processes, to the antibacterial action of Brazilian and Bulgarian propolis, as well as to determine the behavior of these bacteria, according to the incubation period, in medium plus propolis. Dilution of ethanolic extract of propolis in agar was the used method. Brazilian and Bulgarian propolis showed an antibacterial action against all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propolis; Susceptibility profile; Salmonella; Food; Infection.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400006

Susceptibility profile of Salmonella against the antibacterial activity of propolis produced in two regions of Brazil
Provedor de dados: 80
Autores: Orsi,R. O.; Sforcin,J. M.; Rall,V. L. M.; Funari,S. R. C.; Barbosa,Luciano; Fernandes,JR A.
Propolis antibiotic action has been widely investigated. This assay was carried out in order to observe the in vitro antibacterial activity of propolis against Salmonella enteritidis isolated from food and Salmonella typhimurium isolated from human infections. Propolis was collected by Apis mellifera in two regions of Brazil (Mossoró, Rio Grande do Norte State; and Urubici, Santa Catarina State). Both strains survival percentage decreased with time of incubation in Ethanolic Extracts of Propolis (EEP), demonstrating bactericidal effect after 24 hours. It was also observed that EEP from Mossoró was more effective than that from Urubici. The control of the propolis solvent - 70% ethanol - was less effective than EEP, showing only a bacteriostatic effect. We...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propolis; Susceptibility profile; Salmonella; Food; Infection.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200003

Livestock Production and Foodborne Diseases from Food Animals in Thailand
Provedor de dados: 19
Autores: PADUNGTOD, Pawin; KADOHIRA, Mutsuyo; HILL, Glen; 門平, 睦代; ヒル, グレン.
Thailand is a developing nation dependent on agriculture. Due to lack of modern public health practices, she suffers from the consequences of foodborne illnesses. The number of foodborne infection cases has nearly doubled in the past 10 years. Salmonella and Campylobacter pose the greatest risk of bacterial contaminants, mostly from pigs and chickens, and this paper will review livestock production systems and foodborne diseases from cases stemming from these sources. Due to the complexity of the livestock production systems, collection of data to date has been sporadic, but it is clear that controls are needed in slaughterhouse processing methods, and more communication between agencies and surrounding regions is paramount for proper surveillance to have...
Palavras-chave: Campylobacter; Food animal; Foodborne diseases; Salmonella; Thailand.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4127

ESTIMATING THE INCIDENCE OF FOODBORNE SALMONELLA AND THE EFFECTIVENESS OF ALTERNATIVE CONTROL MEASURES
Provedor de dados: 31
Autores: Henson,   Spencer J.
Tipo: Conference Paper or Presentation
Palavras-chave: Foodborne illness incidence; Salmonella; Delphi method; United Kingdom; Food Consumption/Nutrition/Food Safety.
Ano: 1995
URL: http://purl.umn.edu/25967

MEASURING CONSUMER BENEFITS OF FOOD SAFETY RISK REDUCTIONS
Provedor de dados: 31
Autores: Buzby,   Jean C.; Fox,   John A.; Ready,   Richard C.; Crutchfield,   Stephen R.
Microbial pathogens and pesticide residues in food pose a financial burden to society which can be reduced by incurring costs to reduce these food safety risks. We explore three valuation techniques that place a monetary value on food safety risk reductions, and we present a case study for each: a contingent valuation survey on pesticide residues, an experimental auction market for a chicken sandwich with reduced risk of Salmonella, and a cost-of-illness analysis for seven foodborne pathogens. Estimates from these techniques can be used in cost/benefit analyses for policies that reduce food safety risks.
Tipo: Journal Article
Palavras-chave: Contingent valuation; Cost of illness; Experimental auction market; Food safety; Risk reduction; Salmonella; Food Consumption/Nutrition/Food Safety.
Ano: 1998
URL: http://purl.umn.edu/15107

Salmonellosis Control: Estimated Economic Benefits
Provedor de dados: 31
Autores: Roberts,   Tanya.
Salmonellosis, a common human intestinal disorder primarily caused by contaminated meats and poultry, attacks an estimated two million Americans annually. Using a cost of illness approach, the medical costs and productivity losses alone were estimated to cost around one billion dollars in 1987. If pain and suffering, lost leisure time, and chronic disease costs could be quantified, the estimate would increase significantly. Other procedures for calculating the value of life could either raise or lower the estimated economic benefits of reducing human salmonellosis. Incorporating losses to farmers, whose animals have reduced feed efficiency, reduced weight gain, or deaths because of chronic salmonellosis, would also increase the estimates. Also excluded...
Tipo: Working or Discussion Paper
Palavras-chave: Salmonella; Salmonellosis; Foodborne disease costs; Economic costs; Risk assessment; Risk characterization; Food Consumption/Nutrition/Food Safety.
Ano: 1987
URL: http://purl.umn.edu/115797

VALUE-AT-RISK AND FOOD SAFETY LOSSES IN TURKEY PROCESSING
Provedor de dados: 31
Autores: Siaplay,   Mounir; Nganje,   William E.; Kaitibie,   Simeon.
Food safety risks and microbial outbreaks have significant health impacts on society as a whole, as well as economic loss to food processing firms.  According to the U.S. Centers for Disease Control (CDC), an estimated 76 million foodborne illnesses occur each year in the United States.  Of these cases, 325,000 hospitalizations and 5,000 deaths occur each year (Mead et al., 1999).  The U.S. Department of Agriculture, Food Safety and Inspection Services (USDA-FSIS) (1996) estimated that approximately 4,000 deaths each year are attributed to contamination in meat and poultry products.  For food processing firms, microbial outbreaks often result in significant economic losses: food recalls, lost market share, and decreased consumer confidence.  The intangible...
Tipo: Working or Discussion Paper
Palavras-chave: PR/HACCP; Value-at-Risk; Salmonella; Turkey Processing; Food Consumption/Nutrition/Food Safety.
Ano: 2005
URL: http://purl.umn.edu/23556

Costs and Benefits of Two Alternative Salmonella Control Policies in Finnish Broiler Production
Provedor de dados: 31
Autores: Kangas,   Susanna; Lyytikainen,   Tapani; Peltola,   Jukka; Ranta,   Jukka; Maijala,   Riitta.
In this study, costs and benefits of two Salmonella control options in broiler production were compared. The first option was to control Salmonella as set by Council Directive 92/117/EEC, so-called Zoonosis Directive. The second option was more intense control programme, the so-called Finnish Salmonella control programme. The costs of controlling Salmonella in primary and secondary production and the direct and indirect losses due to Salmonella infection in humans in both options were compared. The national control option was found to be economically feasible. One prevented loss of life covered all the control costs of the national programme.
Tipo: Conference Paper or Presentation
Palavras-chave: Salmonella; Broiler; Cost-benefit; Control; Food Consumption/Nutrition/Food Safety.
Ano: 2004
URL: http://purl.umn.edu/24981

SWINE '95 PART II: REFERENCE OF 1995 U.S. GROWER/FINISHER HEALTH AND  MANAGEMENT PRACTICES
Provedor de dados: 31
Autores: Bush,   Eric J.
This report is the second of a three-part release of national information from the second National Animal Health Monitoring System (NAHMS) swine study, the Swine '95 Grower/Finisher. The 1990 National Swine Survey focused on farrowing sows and preweaning piglets. For the Swine '95 Study, the USDA's National Agricultural Statistics Service (NASS) collaborated with Veterinary Services (VS) to select a producer sample that was statistically designed to provide inferences to the nation's swine population. Data collected for the study represented the top 16 pork states and nearly 91 percent of the U.S. hog inventory, as well as nearly three fourths of the nation's pork producers. Data for this report were collected from 418 producers whose operations had 300 or...
Tipo: Report
Palavras-chave: NAHMS; Swine; Monitoring; Epidemiology; Production; Grower/finisher; Feed; Antibiotics; Waste management; Disease; Salmonella; Marketing; Slaughter; Biosecurity; Livestock Production/Industries.
Ano: 1996
URL: http://purl.umn.edu/32770

INTERNATIONAL TRADE AND FOOD SAFETY:   ECONOMIC THEORY AND CASE STUDIES
Provedor de dados: 31
Food safety regulations and the perception of risk are different among countries. This can lead to persistent trade frictions and even reduce food trade. These differences may also lead to increased dialogue between countries, with improved food safety systems the result. Although little disruption to trade has occurred for food safety reasons (considering the total volume of food trade), trade issues or crises related to food safety are wide ranging.  These issues and crises challenge policymakers and industries to both protect domestic food supplies and nurture international markets. Meanwhile, consumers in developed countries are demanding safer food. Risk reduction measures and quality certification programs can not only pre-empt food safety crises,...
Tipo: Report
Palavras-chave: Food safety; International trade; Regulation; Salmonella; BSE; Produce; Seafood; Trade liberalization; Food Consumption/Nutrition/Food Safety; International Relations/Trade.
Ano: 2003
URL: http://purl.umn.edu/33941

Food Safety Information and Food Demand – Effects of Temporary and Permanent News
Provedor de dados: 31
Autores: Smed,   Sinne; Jensen,   Jorgen Dejgaard.
This paper examines the cross-impacts of food safety news concerning one product on the demand for another product, using the Danish demand for pasteurized eggs versus shell eggs as an illustrative case. The study identifies news with a temporary impact and news with a permanent impact on consumers' food demand behavior. The techniques used to identify the permanent versus temporary news are recursive estimation and parameter stability. Whereas "permanent" news is identified to be represented by a specific individual event, "temporary" news concerning salmonella in eggs is aggregated into a news-index variable. Both temporary and permanent news concerning salmonella in shell eggs appear to have significant positive impacts on the demand for pasteurized...
Tipo: Conference Paper or Presentation
Palavras-chave: Salmonella; News; Egg demand; Error correction model; Socio-economic groups; Food Consumption/Nutrition/Food Safety.
Ano: 2002
URL: http://purl.umn.edu/24811

Nutritional and microbiological evaluation of meat and bone meal produced in the state of Minas Gerais
Provedor de dados: 89
Autores: Sartorelli,SA; Bertechini,AG; Fassani,EJ; Kato,RK; Fialho,ET.
The present study investigated the chemical composition and metabolizable energy levels of ten meat and bone meals (MBM) produced in Minas Gerais state (Experiment I) and evaluated the growth performance of broilers fed with diets containing those MBM (Experiment II). In the first experiment, energy values (apparent metabolizable energy [AME] and corrected apparent metabolizable energy [AMEn]) of ten different MBM were determined using the traditional method with total collection of excreta. Four hundred forty 21 day-old Hubbard broilers were used. A reference corn and soybean meal-based diet was replaced in 20% by the feed containing MBM to be tested. A completely randomized experimental design was used with eleven treatments (one reference diet and ten...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broilers; Ingredient; Meat and bone meal; Metabolizable energy; Salmonella.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2003000100007

Potential use of molecular-typing methods for the identification and characterization of salmonella enterica serotypes isolated in the poultry production chain
Provedor de dados: 89
Autores: Baratto,CM; Gelinski,JMLN; Bernardi,AZ; Marafon,A; Braun,F.
Salmonella is widespread in nature and can be found in all links of the poultry production chain. Due to its high impact on meat processing, techniques for the rapid detection and reproducible characterization of Salmonella serotypes in foods are needed. The present study investigated the potential of molecular profiling to identify and differentiate 15 Salmonella serotypes isolated from the poultry production chain, based on 5 primers by random amplified polymorphic DNA (RAPD), enterobacterial repetitive intergenic consensus (ERIC-PCR), amplification of rDNA internal spacer analysis (RISA), and amplified ribosomal DNA restriction analysis (ARDRA) of 16S-23S rRNA internal spacer region (ISR) cleaved with Alu I and Hha I restriction enzymes. Three isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular analysis; Salmonella; Serotypes; Typing.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2012000300003

Serovars of Salmonella spp isolated from broiler chickens and commercial breeders in diverse regions in Brazil from July 1997 to December 2004
Provedor de dados: 89
Autores: Kanashiro,AMI; Stoppa,GFZ; Cardoso,ALSP; Tessari,ENC; Castro,AGM.
Avian salmonellosis is a worldwide problem to the poultry industry, from the point of view of animal health and public health as well. The aim of the present study was to survey the most common Salmonella serovars in commercial breeders or broiler flocks from several regions in Brazil. The results of the present study indicated a high incidence of S enterica subspecies enterica serovar Enteritidis in breeders (57.5%) and broilers flocks (84.0%). The importance of these findings lies in the fact that S. Enteritidis has become the most frequent serovar responsible for foodborne outbreaks and sporadic cases of salmonellosis in humans.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Poultry; Salmonella; Serovars.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2005000300010

Use of lytic bacteriophages to reduce Salmonella Enteritidis in experimentally contaminated chicken cuts
Provedor de dados: 89
Autores: Fiorentin,L; Vieira,ND; Barioni Júnior,W.
Reducing Salmonella contamination in poultry is of major importance to prevent the introduction of this microorganism into the food chain. Salmonellae may spread during storage time (shelf life) whenever pre-harvest control fails or post-harvest contamination occurs. Therefore, preventive measures should also be used in the post-harvest level of poultry production in order to control salmonellae. Chicken skin samples were experimentally contaminated by immersing whole legs (thighs and drumsticks) in a suspension containing 10(6) colony forming units per milliliter (CFU/mL) of Salmonella Enteritidis phage type 4 (SE PT4) at the slaughter day. One day later, samples from one group were immersed in a suspension pool containing 10(9) CFU/mL of each of three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteriophages; Contamination; Salmonella; Poultry; Skin.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2005000400010

Comparação de Meios de Enriquecimento e de Plaqueamento Utilizados na Pesquisa de Salmonella em Carcaças de Frango e Fezes de Aves
Provedor de dados: 89
Autores: Nascimento,MS; Berchieri Jr,A; Barbosa,MD; Zancan,FT; Almeida,WAF.
Este trabalho foi desenvolvido para avaliar, comparativamente, os caldos de enriquecimento Rapapport-novobiocina (RVN), selenito-cistinanovobiocina (SCN) e tetrationato-novobiocina (TN) e os meios para plaqueamento ágar Hektoen (HE), ágar MacConkey (MC), ágar Salmonella-Shigella (SS), ágar verde brilhante (VB) e ágar xilose lisina desoxicolato (XLD), utilizados no isolamento de Salmonella em carcaças de frango e fezes de aves. O procedimento bacteriológico consistiu das etapas de pré-enriquecimento, enriquecimento em caldos seletivos, plaqueamento, testes bioquímicos presuntivos e confirmação sorológica com soros polivalentes anti antígenos somáticos e flagelares de Salmonella.. As fezes foram experimentalmente contaminadas com 8 sorotipos de Salmonella...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Enriquecimento; Plaqueamento; Isolamento; Carcaça de aves; Fezes.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2000000100012

Incidence of Salmonella in imported day-old ducklings. Brazil, 1998-2003
Provedor de dados: 89
Autores: Ribeiro,SAM; Galleti,MCM; Orsi,MA; Ferrati,AR; Mendonça,AO; Doretto Júnior,L; Camillo,SCA; Reischak,D.
The occurrence of Salmonella in a samples of 40 imported day-old duckling flocks was assessed from 1998 to 2003 according to the guidelines of the Brazilian National Poultry Health Program (Programa Nacional de Sanidade Avícola-PNSA). The pathogen was recovered from 26 flocks (65.0%). The most common serovars were S. Saintpaul and S. Kottbus. Up to four serovars were isolated from a single flock. Transportation box swabs (82.6%) and yolk sac pool (47.1%) showed the highest and the lowest frequency of Salmonella isolation, respectively. The high percentage of Salmonella isolation from imported day-old ducklings causes concern because of the zoonotic potential of this agent and its economical importance to commercial poultry breeding.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Imported ducklings; Isolation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000100006

Qualitative and Quantitative Analysis of Salmonella spp. in Broilers Technological Processing and Determination of a Performance Objective (PO) for Frozen Chicken Breast
Provedor de dados: 89
Autores: Machado,SCA; Pereira,VLA; Aquino,MHC; Giombeli,A; Rodrigues,DP; Nascimento,ER.
ABSTRACT The present study investigated the frequency, level of contamination and serotyping of Salmonella strains isolated from broiler flocks in different processing sites and the fulfillment of a Performance Objective (PO) in frozen chicken breasts, as a risk assessment to measure the efficacy of prevention and control programs applied to reduce the risk of Salmonella spp. in raw poultry meat that contribute to reach food safety and public health goals. From 1,800 samples of cloacal swabs, carcasses before and after immersion chilling and frozen breasts derived from 20 broiler flocks slaughtered at two processing plants located in the mid-west and southern regions of Brazil, 278 samples were positive for Salmonella spp. by polymerase chain reaction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler; Chicken meat; Salmonella; Serotype; Enumeration; Performance objective (PO).
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100328

Comparative Evaluation of the Effects of Binzalkonium Chloride, Iodine, Gluteraldehyde and Hydrogen Peroxide Disinfectants against Avian Salmonellae Focusing on Genotypic Resistance Pattern of the Salmonellae Serotypes toward Benzalkonium Chloride
Provedor de dados: 89
Autores: Aksoy,A; El Kahlout,KEM; Yardimci,H.
ABSTRACT Using disinfectants in poultry houses is a common practice to ban the zoonotic pathogens like Salmonella. A major concern in using disinfectants is the emergence of bacteria strains that resist some disinfectants. This phenomenon is manifested in the resistance of some Salmonella serotypes against quaternary ammonium compounds. Such resistance is attributed to qacEΔ1 gene which may be possessed by some Salmonella serotypes. This work aimed to evaluate the resistance of Salmonella serotypes (S. Typhimurium, S. Infantis and S. Entiridis) against different disinfectants (benzalkonium chloride, iodine, gluteraldehyde and hydrogen peroxide). The effect of the disinfectants were evaluated by treatment of the bacteria with different concentrations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Disinfectants; PCR; Poultry; Resistance; Salmonella.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100301

Phenotypic and genotypic resistance profile of Salmonella Typhimurium to antimicrobials commonly used in poultry
Provedor de dados: 89
Autores: Biffi,CP; Stefani,LM; Miletti,LC; Matiello,CA; Backes,RG; Almeida,JM; Neves,GB.
Isolates of Salmonella sp (104) from poultry samples were isolated and serotyped where eleven were identified as Salmonella Typhimurium (ST). ST isolates were phenotypically tested by disk diffusion and minimum inhibitory concentration (MIC). Four genes related to enrofloxacin (GyrA, GyrB, ParC and ParE), two to gentamicin (AadA and AadB) and two to ceftiofur (BlaCMY-2 and AmpC) resistance were searched by PCR. Our results showed ST resistance to all three antibiotics tested (18.1% for ceftiofur, 45.4% for gentamicin, and 18.1% for enrofloxacin) according to the diffusion test. In the MIC test, the ST isolates showed higher levels of resistance (27.2% for ceftiofur, 54.5% for gentamicin, and 18.2% for enrofloxacin). Three resistance genes out of four...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial; Gene; Resistance; Salmonella.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000200013

Influence of Covering Reused Broiler Litter with Plastic Canvas on Litter Characteristics and Bacteriology and the Subsequent Immunity and Microbiology of Broilers
Provedor de dados: 89
Autores: Mesa,D; Lourenço,M; Souza,A; Bueno,A; Pereira,A; Sfeir,M; Santin,E.
ABSTRACT In broiler production, the litter is reused for consecutives flocks, and it is treated during down time between flocks to reduce its microbial load. Although covering the litter with a plastic canvas is a common litter treatment in the field, there is little scientific information available on its efficacy. The aim of this study was to evaluate the effects of covering broiler litter with a plastic canvas for eight days on litter microbiological, physical, and chemical parameters, and on the intestinal microbiota and immunity of broilers. In the first trial, reused litter from a previous flock was distributed into three treatments, with six replicates each: L1 (negative control, litter free from Salmonella Enteritidis (SE) and Eimeria maxima (EM)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ammonia; Cytokines; Lactobacillus; Macrophages; Salmonella.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000400563

Miniaturized most probable number for the enumeration of Salmonella sp in artificially contaminated chicken meat
Provedor de dados: 89
Autores: Colla,FL; Mion,L; Parizotto,L; Rodrigues,LB; Pilotto,F; Dickel,EL; Nascimento,VP do; Santos,LR dos.
Salmonella is traditionally identified by conventional microbiological tests, but the enumeration of this bacterium is not used on a routine basis. Methods such as the most probable number (MPN), which utilize an array of multiple tubes, are time-consuming and expensive, whereas miniaturized most probable number (mMPN) methods, which use microplates, can be adapted for the enumeration of bacteria, saving up time and materials. The aim of the present paper is to assess two mMPN methods for the enumeration of Salmonella sp in artificially-contaminated chicken meat samples. Microplates containing 24 wells (method A) and 96 wells (method B), both with peptone water as pre-enrichment medium and modified semi-solid Rappaport-Vassiliadis (MSRV) as selective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken meat; Most probable number; Salmonella.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000100006

Detection of CD4+ and CD8+ lymphocytes in the intestine of broiler chicks treated with Lactobacillus spp. and challenged with Salmonella enterica serovar Enteritidis
Provedor de dados: 89
Autores: Noujaim,JC; Andreatti Filho,RL; Lima,ET de; Okamoto,AS.
The expression of immune response as a leukocytic infiltrate by CD4+ and CD8+ cells in the epithelium and in the intestinal lamina propria of chicks fed Lactobacillus spp or cecal microflora (CM) and experimentally challenged or not with Salmonella enterica serovar Enteritidis (SE) was studied using immunohistochemistry. Three hundred and twenty day-of-hatch broiler chicks were divided into four groups of 80 birds each and orally received L. reuteri, L. salivarius, L. acidophilus, or CM. Each group was subdivided into four subgroups of 20 birds each, classified as follows: a subgroup did not receive any oral treatment (negative control), subgroup treated with L. spp or CM, subgroup treated with L. spp or CM and challenged with SE, and subgroup only...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken; Immunohistochemistry; Lymphocytes CD4+ and CD8+; Probiotic; Salmonella.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000300008

In-Vitro Adhesion and Invasion Properties of Salmonella Typhimurium Competing with Bacteriophage in Epithelial Cells and Chicken Macrophages
Provedor de dados: 89
Autores: Lee,HY; Biswas,D; Ahn,J.
ABSTRACT This study was designed to assess the role of bacteriophage P22 in the adhesion, invasion, intracellular survival of, and cellular immune response to Salmonella Typhimurium in intestinal epithelial INT-407 and chicken macrophage-like HD11 cells. The ability of S. Typhimurium to adhere, invade, and survive to INT-407 and HD11cells was evaluated under Salmonella infection alone (control), phage treatment followed by Salmonella infection (PS), Salmonella infection followed by phage treatment (SP), and a combination treatment with Salmonella and phage (S+P). The number of S. Typhimurium associated on INT-407 cells was reduced from 4.2 to 2.7 log cfu/cm2 by phage treatment (SP). The number of intracellular S. Typhimurium within INT-407 cells was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adhesion; Bacteriophage; Invasion; Salmonella; Toll-like receptor.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000400427

Immunological evaluation of the intestinal mucosa of broiler chicks treated with Lactobacillus Spp. and challenged with Salmonella Enteritidis
Provedor de dados: 89
Autores: Okamoto,AS; Andreatti Filho,RL; Lima,ET; Pereira,REP; Menconi,A; Rocha,TS; Marietto-Gonçalves,GA.
This study aimed at the antibody production by intestinal mucosa of broilers chicks were orally inoculated with Lactobacillus spp. at one and/or 21 days of age, and subsequently challenged with Salmonella enterica, subspecies enterica, serotype Enteritidis (S. Enteritidis). A total number of 288 drug-free broiler chicks was divided into 6 groups (groups A, B, C, D, E, and F), according to age at Lactobacillus spp. inoculation and SE challenge. The intestinal mucosa immune response was determined as the production of immunoglobulin A against S. Enteritidis, and evaluated by the Enzyme-Linked Immunosorbent Assay (ELISA) technique in intestinal washing fluid. Groups treated with Lactobacillus spp. presented higher IgA production only when the chicks were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicks; IgA; Lactobacillus; Salmonella.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000400009

Effect of Infectious Bursal Disease (Ibd) Vaccines on Infection of Salmonella Heidelberg in Broiler Chickens
Provedor de dados: 89
Autores: Marín-Gómez,SY; Martins,NRS; Fernando,FS; Coelho,HLG; Freitas Neto,OC.
ABSTRACT Salmonella Heidelberg (SH) has represented a great concern to the Brazilian poultry industry in the last years. It is known that immunosuppression in poultry is a contributing factor to increase Salmonella faecal shedding and to disturb control programmes. Not only infectious bursal disease (IBD) virus but also some live vaccines have been reported to induce immunosuppression. In the present study we assessed the effects of two live vaccines against IBD on SH-infected broiler chicks. At 7 days of age, birds of three groups (vaccinated with recombinant HVT-IBD vector, with immune complex-IBD vaccine and unvaccinated) were orally challenged with 1 x 108 CFU of SH. A group of hatchmates remained unvaccinated/unchallenged to serve as negative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Faecal colonization; HVT-VP2 vector; Immune-complex; Depletion.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100321

Occurrence of Salmonella sp in laying hens
Provedor de dados: 89
Autores: Gama,NMSQ; Berchieri Jr,A; Fernandes,SA.
This study was carried out to investigate the presence of Salmonella sp in flocks of white laying hens. In different farms, the transport boxes of twelve flocks were inspected at arrival for the presence of Salmonella. Four positive (A, B, L and M) and one negative (I) flocks were monitored at each four weeks using bacteriological examination of cecal fresh feces up to 52 weeks. Birds were also evaluated at 52 weeks, when 500 eggs were taken randomly, and at 76 weeks, after forced molt. Salmonella enterica serovar Enteritidis and S. enterica rough strain were isolated from the transport boxes of the four positive flocks (flocks A, B, L and M). Salmonella sp was not isolated from the transport boxes or from the feces after 76 weeks-old in flock I....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eggs; Feces; Laying hens; Salmonella.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2003000100002

Extended Spectrum Beta-Lactamase Production and Biofilm Formation in Salmonella Serovars Resistant to Antimicrobial Agents
Provedor de dados: 89
Autores: Mandelli,JZA; Ehrhardt,A; Manto,L; Borges,KA; Furian,TQ; Weber,B; Rodrigues,LB; Santos,LR.
ABSTRACT Antimicrobial resistance is a serious public health problem and Salmonella spp. is highly resistant to antimicrobial agents. Biofilms are important in the food industry due to their formation on products, utensils, and surfaces and the difficulty in their removal. The objective of this study was to assess extended spectrum beta-lactamase (ESBL) production, antimicrobial resistance, and biofilm production of Salmonella isolated from poultry slaughterhouses. Antimicrobial susceptibility was assessed by the disk diffusion assay and ESBL by double diffusion disk assay using the beta-lactamase inhibitor (amoxicillin+clavulanate). The antimicrobials tested were: ampicillin, amoxicillin+clavulanate, aztreonam, ceftazidime, cefotaxime, chloramphenicol,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilms; Drug resistance; ESBL; Salmonella.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200348

In vitro characterization and in vivo properties of Salmonellae lytic bacteriophages isolated from free-range layers
Provedor de dados: 89
Autores: Fiorentin,L; Vieira,ND; Barioni Júnior,W; Barros,S.
Occurrence of food poisoning related to Salmonella-contaminated eggs and chicken meat has been frequent in humans. Salmonella Enteritidis (SE) and Salmonella Typhimurium (ST) are included among the most important paratyphoid salmonellae associated with chicken meat and eggs. Elimination of Salmonella at the pre-harvest stage can play a significant role in preventing the introduction of this pathogen into the food chain and consequently in the reduction of food poisoning in humans. Bactericidal bacteriophages may provide a natural, nontoxic, feasible and non-expensive component of the multi-factorial approach for a pre-harvest control of Salmonella in poultry. Five bacteriophages lytic for SE PT4 and ST were obtained from 107 samples of feces of free-range...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteriophages; Food poisoning; Poultry; Salmonella.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200009

Serotyping and Genotyping of Salmonella Strains Isolated From Broilers, Chicken Carcasses Before and After Chilling, and Frozen Chicken Breasts Produced in The States of Mato Grosso do Sul and Santa Catarina, Brazil
Provedor de dados: 89
Autores: Machado,SCA; Pereira,VLA; Aquino,MHC; Santos,AFM; Rodrigues,DP; Giombelli,A; Nascimento,ER.
ABSTRACT The present study investigated the effectiveness of a single Salmonella prevention and control program applied in two different processing plants, located in the states of Mato Grosso do Sul (plant A) and Santa Catarina (Plant B), belonging to the same company, and identified Salmonella strain subtypes isolated from broilers, carcasses before and after chilling, and frozen chicken breasts. The Salmonella prevention and control program was 90% effective in plant A and 100% in plant B, considering a level of 10% positive samples per frozen chicken breast batch acceptable. A total of 128 strains were serotyped, being 10 from drag swabs, 31 from cloacal swabs, 83 from carcasses, and 4 from frozen chicken breasts. After serotyping analysis, 30 strains...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food safety; PFGE; Poultry meat; Salmonella.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000100135

Desempenho de quatro sistemas para compostagem de carcaça de aves
Provedor de dados: 91
Autores: Costa,Mônica S. S. de M.; Costa,Luiz A. de M.; Pelá,Adilson; Silva,Cesar J. da; Decarli,Léo D.; Matter,Uilson F.
Objetivou-se avaliar o desempenho de quatro sistemas para compostagem de carcaça de aves pelo monitoramento diário da temperatura, análise da presença ou ausência de Salmonella sp., contagem de coliformes totais (CT) e fecais (CF) e reciclagem de nutrientes. Os sistemas avaliados foram: Leira com aeração (LCA), Leira sem aeração (LSA), Composteira com aeração (CCA) e Composteira sem aeração (CSA). O processo de compostagem foi conduzido em dois estágios. No primeiro, as carcaças foram intercaladas com cama de aviário, palha e água. No segundo estágio, a massa de compostagem foi transferida para um pátio onde o material foi revolvido e umedecido semanalmente até a estabilização. Detectou-se a presença de Salmonella sp nos sistemas LSA e CSA ao final do...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Temperatura; Salmonella; Coliformes totais e fecais; Reciclagem de nutrientes.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662006000300023

Consórcio suíno-peixe: aspectos ambientais e qualidade do pescado
Provedor de dados: 96
Autores: Pilarski,Fabiana; Tomazelli Júnior,Osmar; Casaca,Jorge de Matos; Garcia,Flávio Roberto Mello; Tomazelli,Ingrid Boesche; Santos,Ieda Rottava dos.
Amostras de músculo de carpa comum (Cyprinus carpio) e de água de três viveiros fertilizados com dejetos de suínos e de um viveiro em que os peixes foram alimentados com ração comercial para peixes foram analisadas durante seis meses. O objetivo foi observar a presença de bactérias patogênicas, como Salmonella, Staphylococcus aureus e coliformes totais e fecais, em peixes comercializados in natura no município de Chapecó, Santa Catarina. Foram aferidos quinzenalmente, na água dos quatro viveiros, oxigênio dissolvido, pH, turbidez, alcalinidade total, dureza total, amônia, nitrato e ortofosfato, bem como a quantidade de coliformes totais e fecais. A análise microbiológica músculo dos peixes demonstrou ausência de Salmonella em todas as amostras. A contagem...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyprinus carpio; Dejetos suínos; Salmonella; Staphylococcus aureus; Coliformes totais e coliformes fecais.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982004000200001

Effect of Artemisia apiacea Hance on growth performance, cecal opportunistic bacteria, and microbicidal peptides in rabbits
Provedor de dados: 96
Autores: Ding,Ke; Wang,Jianping; Liu,Ning; Zhang,Feike.
ABSTRACT This study aimed to investigate the effect of Artemisia apiacea Hance supplementation on growth performance, cecal opportunistic bacteria, and antimicrobial defense using 120 rabbits. There were four experimental diets containing a control and A. apiacea Hance added at doses of 25, 50, and 75 g kg−1 of feed. The trial lasted for 70 days. The results showed that diets supplemented with A. apiacea Hance improved feed intake, body weight gain, and feed efficiency. Linear and quadratic responses were found between feed intake and herbal meal doses. For cecal opportunistic pathogenic bacteria, compared with the control treatment, the herb decreased cecal C. perfringens, Gram-negative bacteria, and Salmonella spp. by 9.5 to 56.8%. Linear responses of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C. perfringens; Gene expression; Salmonella.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100533

Effect of chitosan coating on contamination of fresh bovine meat sold in the open market
Provedor de dados: 124
Autores: Silva,Alessandra Santana; Sampaio,Adriana Pereira; Santos,Marly Silveira; Souza,Bartolomeu Warlene Silva de; Evangelista-Barreto,Norma Suely.
ABSTRACT This study aimed to evaluate the sanitary status of fresh bovine meat sold at the open market in Jiquiriça municipality, Brazil and validate the use of chitosan as an antimicrobial coating to control the bioburden. In total, we evaluated 30 samples of fresh beef for the presence of coagulase-positive staphylococci and Salmonella spp. Furthermore, stores selling fresh meat products were evaluated for compliance with sanitary regulations set by the Ministry of Health in Brazil. To evaluate the antimicrobial activity of chitosan two sets of meat samples were tested, the test group consisted of contaminated meat coated with 2% chitosan solution whereas the control group consisted of untreated contaminated meat. It was found that fresh bovine meat at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Staphylococcus; Internal meat temperature; Antimicrobial activity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902019000100038

Salmonella serovars isolated from poultry material from 1994 to 1999
Provedor de dados: 195
Autores: Andreatti Filho, Raphael Lúcio; Fernandes, Sueli Aparecida; Boretti, Lígia Pedroso; Barros, Mércia Rodrigues; Del Bem, Sandro Roberto; Fontana, Adriano; Sampaio, Homero Marcos; Savano, Edson Naoto.
Salmonella serovars isolated from poultry material from different geographical areas were identified in the 1994-1999 period. A total of73 serovars was isolated, with recognition of21 different serovars distributed in nine serogroups. Enteritidis was the serovar with highest incidence, 46.57%, and was isolated from feed, litter and meat, poultry byproducts and fish meal. Natum had the second highest incidence, 6.84%, and 80% of isolations were made from meat meal. S. pullorum represented 2.73% of the isolations, 100% from brown layers, confirming their susceptibility to infection by this serovar. Of the materials included in the present study, broilers and meat meal had more Salmonella spp., 41.09% and 30.13%, respectively. Among the serovars isolated from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Poultry; Serovars; Serogroups; Isolation Salmonella; Aves; Sorovares; Sorogrupos; Isolamento.
Ano: 2001
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3310

Resistencia antimicrobiana en cepas de Salmonella enterica subsp. enterica aisladas en carnes de aves importadas
Provedor de dados: 101
Autores: Sonali Rivera Corona,Marieta; Granda,Ana Ernestina; Felipe,Laudelina; Bonachea,H.
La carne de ave importada contiene peligros como Salmonella, la cual pude conllevar a riesgos en la población consumidora. Este estudio se llevó a cabo con el fin de detectar la presencia de cepas de Salmonella enterica subsp. enterica resistente a los antibióticos en carnes de ave importada, que pueden pasar a la cadena alimentaria. Se analizaron 3132 muestras. La investigación se realizó según metodología establecida en normas cubanas. Se demostró el peligro Salmonella enterica subsp. enterica en carnes de aves importadas. Los serogrupos más frecuentes correspondieron a C2, B y D. Las cepas de Salmonella enterica subsp. enterica aisladas de las carnes de ave importadas mostraron resistencia antimicrobiana en una alta proporción.
Tipo: Journal article
Palavras-chave: Salmonella; Peligros; Aves; Resistencia antimicrobiana.
Ano: 2012
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2012000200010

Mixture of formic and propionic acid as additives in broiler feeds
Provedor de dados: 63
Autores: Vale,Marcos Martinez do; Menten,José Fernando Machado; Morais,Sônia Cristina Daróz de; Brainer,Mônica Maria de Almeida.
The presence of Salmonella species in feeds and ingredients is an important source of salmonella contamination for animals. Organic acid mixtures have shown to be an effective alternative to eliminate salmonellas in feeds and chickens. In the present study, the performance of male broiler chickens receiving levels (0, 0.25, 0.50, 1.0 and 2.0%) of a mixture of organic acids (OAs) (70% formic acid and 30% propionic acid) in the diet was evaluated from the age one to 42 days, in a completely randomized experimental design with five treatments and five replications of 40 birds each. Diets were based on corn, soybean meal and soybean oil. Body weight, weight gain and feed intake from the age one to 21 days were affected by the treatments; 2% OAs in the diet...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Chicken; Organic acids; Food safety.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000400004

PESQUISA DE Salmonella spp. EM LEITE CRU, LEITE PASTEURIZADO TIPO C E QUEIJO "MINAS FRESCAL" COMERCIALIZADOS NO MUNICÍPIO DE PIRACICABA - SP
Provedor de dados: 63
Autores: ÁVILA,C.R. de; GALLO,C.R.
Este trabalho foi conduzido para detectar a ocorrência de Salmonella spp. em leite cru, leite pasteurizado tipo C e queijo "Minas Frescal". Analisaram-se 19 amostras de cada produto de diferentes marcas comerciais, adquiridas em diversos estabelecimentos comerciais em Piracicaba, SP. Segundo o padrão de amostragem e métodos adotados, não foi detectada a presença de Salmonella spp. nas amostras analisadas. Durante este trabalho, foi possível observar que os 3 meios de isolamento utilizados: agar verde brilhante, agar bismuto sulfito e agar Salmonella-Shigella, apresentaram baixa seletividade, uma vez que permitiram o crescimento de bactérias não pertencentes ao gênero Salmonella. O mesmo ocorreu com os caldos tetrationato e selenito-cistina utilizados no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Leite cru; Leite pasteurizado; Queijo "Minas Frescal".
Ano: 1996
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161996000100023

UTILIZAÇÃO DA PRÓPOLIS E ÁLCOOL ETÍLICO NO CONTROLE DE Salmonella EM RAÇÕES AVÍCOLAS
Provedor de dados: 63
Autores: MAZZUCO,H.; SILVA,R. D. de M. e; BERCHIERI Jr.,A.; OLIVEIRA,E. de.
Em quatro experimentos foram avaliados como agentes antibacterianos os produtos própolis em solução alcoólica e álcool etílico, adicionados às rações artificialmente contaminadas com os respectivos sorotipos: Salmonella typhimurium Nalr - Specr, (resistentes ao ácido Nalidíxico e a Spectinomicina) nos três primeiros experimentos e Salmonella agona Nalr - Specr, Salmonella infantis Nalr - Specr e Salmonella enteritidis Nalr - Specr no quarto experimento. As rações foram fornecidas a grupos de 10-16 pintos de corte de um dia. Em todos os experimentos os produtos testados foram adicionados na base de 2% da ração. Quando se utilizou solução hidroalcóolica de própolis (exp. 1), seguidas 120 horas após o desafio, detectou-se a presença da bactéria nos cecos. No...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Rações avícolas; Controle; Própolis; Álcool etílico; Frangos de corte.
Ano: 1996
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161996000100001

Prevalência de enterobactérias em Bothrops jararaca no Estado de São Paulo: levantamento microbiológico e padrões de resistência antimicrobiana - DOI: 10.4025/actascibiolsci.v30i3.536
Provedor de dados: 3
Autores: Bastos, Henrique Marçal; Lopes, Luiz Fernando Larangeira; USP - São Paulo; Gattamorta, Marco Aurélio; UNICAMP - Campinas; Matushima, Eliana Reiko; USP - São Paulo.
Existem poucos levantamentos microbiológicos em répteis no Brasil. O estudo aqui descrito enfoca uma espécie de serpente de grande interesse médico e serve como base para outros estudos e comparações. As amostras foram colhidas diretamente do cólon de jararacas adultas e saudáveis. O material foi semeado em ágar MacConkey (Difco®) e ágar XLT4 (Difco®). Os isolados foram identificados por meio do Sistema API 20E de Identificação Bioquímica (Galerias  API-BioMériux®). Os isolados de Salmonella sp. foram submetidos à sorotipagem. Por fim, as colônias foram submetidas ao teste de sensibilidade antimicrobiana. Foram obtidos vários gêneros da família Enterobacteriaceae (Citrobacter, Enterobacter, Escherichia, Klebsiella, Kluyvera, Morganella, Proteus,...
Palavras-chave: 2.00.00.00-6    Ciências Biológicas Bothrops jararaca; Snake; Reptile; Intestinal microbiota; Enterobacteriaceae; Salmonella. Bothrops jararaca; Snake; Reptile; Intestinal microbiota; Enterobacteriaceae; Salmonella.
Ano: 2008
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/536

Control de bacterias patógenas y hongos de postcosecha con extractos del pigmento de Gibberella zeae (Fusarium graminearum)
Provedor de dados: 115
Autores: Villanueva-Arce,Ramón; Aguilar-Pompa,C. Arturo; Gómez y Gómez,Y. de las Mercedes; Valencia-Del Toro,Gustavo; Piña-Guzmán,A. Belem; Bautista-Baños,Silvia.
El objetivo del estudio fue evaluar efectos del extracto etanólico crudo del pigmento rojo-púrpura producido por Gibberella zeae (Fusarium graminearum) en el control de las bacterias Bacillus subtilis, Salmonella typhi, Shigella sp., Staphylococcus aureus, Escherichia coli, la levadura Candida albicans, y los hongos Colletotrichum acutatum, C. fiagariae y C. gloeosporioides. El diseño experimental fue completamente al azar con un arreglo factorial 3x2 de tratamientos: temperatura (20; 28 °C), pH (6; 8) y medio de cultivo sólido (PDA; EMA; Czapeck) y líquido (caldo papa; caldo extracto de malta y caldo Czapeck). Las variables de respuesta fueron tipo y hábito de crecimiento del micelio, color de la colonia, diámetro de la colonia (mm), tiempo (d) de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pigmentos fúngicos; Aurofusarina; Salmonella; Shiguella; Colletotrichum.
Ano: 2013
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952013000700006

Phenotypic and genotypic characterization of Salmonella spp. isolated from foods and clinical samples in Brazil
Provedor de dados: 42
Autores: MIRANDA,ANDREA L.; CORDEIRO,SORAIA M.; REIS,JOICE N.; CARDOSO,LUCAS G.; GUIMARÃES,ALAÍSE G.
ABSTRACT Increasing antimicrobial resistance in Salmonella species has been a serious problem for public health worldwide. This study examines Salmonella spp. recovered from foods and clinical samples on serotype, antimicrobial resistance and PFGE genotypes. It identified 91 salmonellae, belonging to 31 different serotypes, from 36 isolates from food and 55 clinical samples. Salmonella Infantis (16.5%) and Salmonella Enteritidis (13.7%) are the most common among food isolates, whereas Salmonella Enteritidis (29.0%) and Salmonella Typhimurium (16.0%) mainly causes human salmonellosis. Antimicrobial susceptibility data showed that 63.0% of the isolates were fully susceptible to 12 antibiotics tested. Nalidixic acid showed high resistance rates, 32.7% and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Salmonella Infantis; Salmonella Enteritidis; Antimicrobial resistance.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301143

Salmonella enterica: isolamento e soroprevalência em suínos abatidos no Rio Grande do Sul
Provedor de dados: 45
Autores: Schwarz,P.; Calveira,J.; Sella,A.; Bessa,M.; Barcellos,D.E.S.N.; Cardoso,M.
Estudou-se a prevalência de Salmonella spp. em suínos ao abate e compararam-se os resultados obtidos no isolamento e na sorologia de modo a determinar a fase mais importante de infecção (granja ou transporte/espera pré-abate) em relação ao número de suínos portadores encontrados ao abate. Foram avaliados 40 rebanhos de três diferentes empresas do sul do Brasil, com colheitas de sangue e linfonodos mesentéricos de 20 animais por lote. O soro foi submetido ao ELISA produzido com antígeno somático de Salmonella Typhimurium. A frequência de isolamento variou de 62,5% a 85,0%, enquanto a soroprevalência de 73,8% a 83,2% nos três sistemas amostrados. Os sorovares mais prevalentes foram Agona, Typhimurium e Panama. A infecção dos animais ocorreu nas granjas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Suíno; Salmonella; Prevalência; Isolamento; Sorologia.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000500003

Listeria monocytogenes, E. coli O157, Salmonella spp. e microrganismos indicadores em carcaças bovinas para exportação
Provedor de dados: 45
Autores: Matos,A.V.R.; Nunes,L.B.S.; Vianna,C.; Spina,T.L.B.; Zuim,C.V.; Possebon,F.S.; Xavier,D.M.; Ferraz,M.C.; Pinto,J.P.A.N.
Foram colhidas amostras de 100 carcaças em um frigorífico exportador, localizado no interior do estado de São Paulo, amostradas ao longo de um ano, por meio do método de esponjas, aplicado na região do peito do animal. As amostras foram colhidas em três pontos, denominados A, B e C, sendo cada carcaça amostrada nos três pontos, localizados nas etapas: pós-sangria (A); pós-esfola (B) e pós-lavagem (C). Foram realizadas pesquisas de Listeria sp., E. coli O157, Salmonella spp. e microrganismos indicadores (Petrifilms® AC, EC e EB). Não foram isolados Listeria ou E. coli O157 em nenhuma das 300 amostras. Salmonella spp. foi isolada em nove, sendo oito no ponto A e uma no ponto B. Para mesófilos, as contagens variaram de 0 a 6,8 log UFC/cm², para coliformes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovinos; Salmonella; E. coli; Listeria; Microrganismos indicadores.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000400007

Sanitizer resistance of biofilm-forming Salmonellaisolated from meat products
Provedor de dados: 45
Autores: Milan,C.; Agostinetto,A.; Conceição,R.C.S.; Gonzalez,H.L.; Timm,C.D.
Este estudo avaliou a capacidade de Salmonella enterica subsp. enterica isolada de produtos cárneos formar biofilme e testou sua resistência a diferentes sanitizantes. Vinte cepas foram avaliadas quanto à capacidade de formar biofilme em placas de microtitulação. As cepas formadoras de biofilme foram testadas em superfícies de polietileno de alta densidade, aço inoxidável e vidro e tiveram a sensibilidade ao hipoclorito de sódio e ao iodo avaliada. Duas cepas de Salmonella Enteritidis isoladas de produtos de frango apresentaram capacidade de formar biofilme nas superfícies testadas. Essas cepas alcançaram maiores populações nas superfícies do que aquelas não formadoras de biofilme, e foram mais difíceis de remover ou reduzir. Devido à ação sanitizante ser...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Biofilm; Salmonella; Glass; Stainless steel; High-density.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200642

Genes de virulência e diversidade genética em Salmonella spp. isoladas de amostras de origem suína
Provedor de dados: 45
Autores: Moura,M.S.; Oliveira,R.P.; Melo,R.T.; Mendonça,E.P.; Fonseca,B.B.; Rossi,D.A.
A diversificação da produção industrial de alimentos de origem suína e o intercâmbio comercial de animais e seus derivados destinados ao consumo humano podem ser importantes disseminadores de sorovares de Salmonella spp. na cadeia alimentar. Objetivou-se avaliar em 86 cepas de Salmonella spp., isoladas em granja de terminação e no abate de suínos, a ocorrência de três genes de virulência (invA, agfA e lpfA), bem como a similaridade genética entre elas. A ocorrência do gene invA foi verificada em 100% das amostras. O gene lpfA foi detectado em 80,23% (69/86) das cepas, não foi detectado em S. Panama e estava presente em todas as cepas de S. Infantis. O gene agfA foi detectado em 63,95% (55/86) das amostras. S. Agona apresentou positividade para todos os...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Suíno; Genes; RAPD-PCR; Salmonella; Virulência.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000501367

A Lactobacillus rhamnosus strain induces protection in different sites after Salmonella enterica subsp. enterica serovar Typhimurium challenge in gnotobiotic and conventional mice
Provedor de dados: 45
Autores: Mendonça,A.H.; Cerqueira,M.M.O.P.; Nicoli,J.R.; Sousa,S.M.M.; Nardi,R.M.D.; Souza,F.N.; Fonseca,L.M.; Leite,M.O.; Arantes,R.M.E.
The ability of a Lactobacillus rhamnosus strain isolated from a healthy breast-fed human newborn to reduce the pathological consequences for the host due to an experimental oral infection with Salmonella enterica subsp. enterica serov. Typhimurium in vivo was determined using gnotobiotic and conventional mice. Conventional mice received 0.1mL probiotic milk (8.0 log colony-forming unit) daily for 10 days before the oral pathogenic challenge (5.0 log colony-forming unit). Then probiotic treatment was continued until the end of the experiment. Probiotic treatment in germ-free mice consisted of a single dose of the probiotic milk at the beginning of the experiment and a challenge with S. Typhimurium 10 days later (3.0 log colony-forming unit). A protective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal infection; Lactobacilli; Probiotics; Salmonella.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000200004

Detecção sorológica e microbiológica de Salmonella spp. em emas (Rhea americana)
Provedor de dados: 45
Autores: Pereira,R.A.; Macagnan,M.; Canal,C.W.; Schmidt,V.
The fast agglutination screening test, using S. Typhimurium as the antigen, was compared with the standard bacterial method to identify rheas (Rhea americana) contaminated with Salmonella spp.at slaughter.Seventy birds were serologically tested for Salmonella enterica Pullorum using a commercial antigen.Of these, 66 were also submitted to a macroagglutination test, using a strain of Salmonella Typhimurium isolated from rheas.All birds did not react with the commercial S. Pullorum antigen, but 37 were positive for the FAST-ST.The isolation of Salmonella spp. was verified in 66 (94.2%) birds. 85.7% were found in liver samples, 60% in feces and 42.3% in cloacal swabs.A total of 16.6% were identified as being S. enterica enterica rugosa, 35.9% as S....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Diagnóstico; Soroaglutinação; Método microbiológico padrão; Salmonella; Rhea americana.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400040

Qualidade microbiológica de queijo-coalho comercializado em Aracaju, SE
Provedor de dados: 45
Autores: Santana,R.F.; Santos,D.M.; Martinez,A.C.C.; Lima,Á.S.
Determinou-se a ocorrência de Staphylococcus aureus, Salmonella spp. e de microrganismos indicadores de contaminação fecal em queijos-coalho comercializados em 15 pontos de venda do Mercado Central de Aracaju, SE, durante quatro meses. Dezesseis amostras (26,7%) foram positivas para Salmonella spp. e 28 (46,7%) positivas para estafilococos coagulase positiva. Quanto à contaminação por coliformes totais, 56 (93,3%) das amostras apresentaram valores entre 8,0 x10² e 1,23 x10(4)NMP/g, e de 2,72 x10² a 1,12 x10³NMP/g para coliformes termotolerantes. Estes valores não atenderam à legislação brasileira para queijo-coalho e as amostras analizadas podem ser classificadas como impróprios ao consumo humano.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Queijo-coalho; Salmonella; Coliformes; Staphylococcus coagulase positivo.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000600031

Utilização do yacon ( Smallanthus sonchifolius ) na proteção contra colonização intestinal de frangos de corte infectados por Salmonella Enteritidis
Provedor de dados: 45
Autores: Oliveira,M.G.X.; Porretta,M.C.; Itaya,N.M.; Oliveira,M.C.V.; Reple,J.N.; Cunha,M.P.V.; Sanches,L.A.; Davies,Y.M.; Menão,M.C.; Borges,J.C.S.; Polaquini,L.E.M.; Knöbl,T.
RESUMO Os frutanos do tipo inulina são oligossacarídeos que favorecem a multiplicação de determinados gêneros bacterianos no intestino, promovendo um efeito prebiótico. Este trabalho avaliou o efeito da inulina extraída de raízes de yacon (Smallanthus sonchifolius) sobre a colonização intestinal de frangos de corte experimentalmente infectados por Salmonella Enteritidis. Sessenta frangos de corte com um dia de idade foram divididos em três grupos de tratamento, com duas repetições, criados até 21 dias. As aves do grupo yacon receberam 100mg de inulina/dia, via oral, por três dias consecutivos. No sétimo dia de vida, as aves tratadas e o controle positivo foram desafiados pela via oral com uma cultura de S. Enteritidis. Não foram observadas diferenças de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avicultura; Inulina; Prebióticos; Salmonella.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000300695

Infecção por Salmonella enterica em suínos criados em um sistema integrado de produção do sul do Brasil
Provedor de dados: 45
Autores: Silva,L.E.; Gotardi,C.P.; Vizzotto,R.; Kich,J.D.; Cardoso,M.R.I.
Avaliou-se a difusão da infecção em um rebanho com prévio isolamento de Salmonella sp, em que leitões, individualmente identificados, foram amostrados para excreção fecal de Salmonella sp e sorologia do nascimento ao abate. Da mesma forma, amostras de ração e suabes do ambiente foram coletados durante o estudo para pesquisa de Salmonella sp A pesquisa de anticorpos foi realizada pela utilização de ELISA-LPS de Salmonella Typhimurium. Os leitões foram negativos na análise bacteriológica e na sorologia até a fase de creche, tornando-se positivos para Salmonella sp no início da terminação. Nessa amostragem, 28,6% dos animais foram soropositivos e 75% estavam excretando Salmonella sp nas fezes. Ao abate, a percentagem de animais soropositivos (76,9%) aumentou,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Suíno; Estudo longitudinal; Salmonella; ELISA.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000400001

Uso de microbiota cecal congelada com crioprotetores em pintos infectados experimentalmente com Salmonella enterica sorovar Enteritidis
Provedor de dados: 45
Autores: Andreatti Filho,R.L.; Lima,E.T.; Okamoto,A.S.; Sampaio,H.M.
Pintos de corte com um dia de idade foram tratados com microbiota cecal cultivada em condição de aerobiose, nos tempos de congelamento de 90, 200, 290 e 360 dias, e associada aos crioprotetores sacarose, trealose, dimetilsulfóxido (DMSO) e glicerol. Posteriormente as aves foram desafiadas com Salmonella Enteritidis, visando determinar a eficácia dos tratamentos em relação à quantidade de bactérias viáveis da microbiota que foi maior aos 90 dias (10,58 Log10 UFC/ml), quando as aves foram tratadas com sacarose, e menor aos 290 dias, quando tratadas com glicerol (7,73 Log10 UFC/ml). No tempo zero, todas as aves apresentaram Salmonella (100%) quando tratadas com DMSO e glicerol, com colonização cecal de 4,9 e 5,2 Log10 UFC/g do conteúdo cecal, respectivamente;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pinto; Microbiota cecal; Salmonella; Exclusão competitiva; Crioprotetor.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000300015

Avaliação in vitro da atividade inibitória de Lactobacillus spp., isolados do inglúvio e cecos de aves sobre Salmonella
Provedor de dados: 45
Autores: Barros,M.R.; Andreatti Filho,R.L.; Lima,E.T.; Crocci,J.A.
Inglúvios e cecos de reprodutoras comerciais de frangos de corte foram utilizados para o isolamento de Lactobacillus spp. As estirpes isoladas foram caracterizadas como Gram-positivo, catalase negativo, produtoras de gás em glicose, não produtoras de H2S em triple sugar iron e identificadas pela reação em cadeia da polimerase como Lactobacillus reuteri e Lactobacillus salivarius. A utilização da técnica spot-on-the-lawn para avaliação da inibição in vitro permitiu a análise de vários microrganismos simultaneamente. Todas as estirpes isoladas inibiram in vitro S. Enteritidis fagotipo 4, S. Enteritidis fagotipo 28, S. Typhimurium, S. Pullorum, S. Agona, S. Anatum, S. Dublin e S. Senftenberg.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reprodutora de frango de corte; Identificação; Lactobacillus; Salmonella; PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000400013

Ocorrência de Campylobacter e Enterobacteriaceae em aves silvestres e frangos de corte
Provedor de dados: 45
Autores: Dias,P.A.; Moraes,T.P.; Wilsmann,D.E.; Ferrasso,M.M.; Marinheiro,M.F.; Heinen,J.G.; Calabuig,C.I.P.; Timm,C.D.
RESUMO As aves silvestres podem ser reservatório de bactérias patogênicas e atuar como veiculadoras desses microrganismos para o ambiente, os animais domésticos e o homem. Portanto, o objetivo deste trabalho foi verificar a ocorrência de Campylobacter spp., Yersinia enterocolitica e Salmonella enterica em aves silvestres capturadas nas áreas próximas de aviários e em frangos de corte alojados nesses estabelecimentos, além de verificar a presença dos genes cdtA, cdtB e cdtC nos isolados de Campylobacter e identificar os sorotipos de Salmonella encontrados. Amostras de fezes de 189 aves silvestres capturadas com redes de neblina nas áreas próximas de 10 aviários e de 200 frangos de corte foram processadas para pesquisa de Campylobacter spp., S. enterica e Y....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Campylobacter; Salmonella; Zonotrichia capensis; Sicalis flaveola.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100225

Prevalence and identification of Salmonella spp. in water buffaloes from São Paulo State, Brazil
Provedor de dados: 45
Autores: Silva,D.G.; Santana,A.M.; Maluta,R.P.; Pizauro,L.J.L.; Bernardes,P.A.; Aquino,G.V.; Benincasa,N.C.; Rodrigues,D.P.; Fagliari,J.J.
RESUMO O objetivo do estudo foi investigar a prevalência de Salmonella spp. em amostras de fezes de búfalos do estado de São Paulo, Brasil, e identificar os sorotipos isolados. Foram examinadas 116 amostras de suabes retais de búfalos das raças Jafarabadi e Murrah, coletadas em triplicata, em seis propriedades rurais localizadas nas regiões Central, Centro-Oeste e Nordeste do estado de São Paulo, Brasil. Para avaliar a presença de Salmonella spp., foram utilizados três diferentes caldos de enriquecimento (caldo selenito cistina, caldo tetrationado Muller-Kauffmann e caldo Rappaport-Vassiliadis) e dois diferentes meios de cultura (ágar verde brilhante modificado e ágar XLT4). Das 116 amostras de suabes retais examinadas, oito amostras (6,90%; 8/116) foram...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bubalus bubalis; Enrichment broth; Feces; Salmonella; Serotype.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000501645

Contaminação por Salmonella spp. em uma cadeia de produção de ovos de uma integração de postura comercial
Provedor de dados: 45
Autores: Kottwitz,L.B.M.; Back,A.; Leão,J.A.; Alcocer,I.; Karan,M.; Oliveira,T.C.R.M.
Contamination of ISA BROWN layer hens by Salmonella spp. and serotyping of the isolated strains were performed in 30 farms. Salmonella detection was done from feces and cloaca swabs. Eggs produced in the farms contaminated by Salmonella spp. were analyzed by culturing the shell rinsing and egg yolk. Salmonella spp. was isolated in eight (23.0%) farms, but was not detected from eggs. S. Enteritidis, which is the most prevalent sorotype associated to human salmonelosis in Paraná State, was not isolated either from birds or eggs analyzed. The sorotypes isolated were Salmonella enterica subsp. enterica (0:3.10) (67.0%), Mbandaka (11.0%), Infantis (11.0%), and Newport (11.0%).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovo; Salmonella; Contaminação.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200034

Sistema de lavagem de carcaças e controle de Salmonella spp. em abatedouros de frangos de corte
Provedor de dados: 45
Autores: Isolan,L.W.; Perdoncini,G.; Todeschini,B.; Santos,L.R.; Guahyba,A.S.; Depner,R.; Nascimento,V.P.
RESUMO Avaliaram-se os dados do controle e monitoramento de Salmonella em carcaças de frangos de corte, antes e após a implantação do sistema de lavagem de carcaças. Foram amostradas 2692 carcaças antes da instalação do sistema e 1940 após a instalação, totalizando 4632 amostras em cinco abatedouros sob Inspeção Federal, no sul do Brasil. Anteriormente à instalação dos lavadores, obtiveram-se 156 resultados positivos para Salmonella spp. e, após a instalação, 83 resultados positivos, com diferença significativa (P&lt;0,05/OR 1,4) entre os resultados gerais. Em dois dos cinco abatedouros avaliados, houve redução na positividade para Salmonella spp. nas carcaças amostradas após a instalação do lavador. Entretanto, em três estabelecimentos não houve diferença...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Contaminação cruzada; Controle; Monitoramento; Salmonella.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100252

Isolation of Salmonella spp. in captive Psittaciformes from zoos and a commercial establishment of Fortaleza, Brazil
Provedor de dados: 45
Autores: Lopes,E.S.; Cardoso,W.M.; Albuquerque,Á.H.; Teixeira,R.S.C.; Salles,R.P.R.; Bezerra,W.G. A.; Rocha e Silva,R.C.; Lima,S.V.G.; Sales,R.J.P.F.; Vasconcelos,R.H.
This study showed a low prevalence of Salmonellaspp. in captive psittacines from zoos and a commercial establishment of Fortaleza. None of the isolated serotypes (S. Lexington, S. Saintpaul and S. Newport) have yet been reported in Amazona aestiva, Ara chloropteraor Melopsittacus undulatus. However, the fact that most birds presented negative for Salmonellaspp. may not imply the absence of this pathogen in these birds, since the intermittent excretion is a well-known characteristic of this microorganism.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Psittaciformes; Amazona aestiva; Ara chloroptera; Melopsittacus undulatus.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300965

Rapid detection of Salmonella sp. in pork samples using fluorescent in situ hybridization: a comparison with VIDAS®-SLM system and ISO 6579 cultural method
Provedor de dados: 45
Autores: Vieira-Pinto,M.; Oliveira,M.; Bernardo,F.; Martins,C.
This study reports the use of the fluorescent in situ hybridization (FISH) with Sal3 probe for Salmonella detection in swine carcasses inner surface (swab); and in the correspondent samples of ileum, ileocolic, and mandibular lymph nodes; and tonsils, after dilution (1:10) in buffered peptone water and a pre-enrichment step (37(0)C, 18h). In order to evaluate the efficiency of FISH, 235 naturally contaminated samples were simultaneously tested by the cultural method (ISO 6579) and by the Vitek Immuno Diagnostic Assay System (VIDAS®) - Salmonella (SLM) system. The cultural method identified 39 positive samples. From these, VIDAS®- SLM only detected 23. FISH identified 115 positive samples. This difference was highly significant (P&lt;0.001). From positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swine; Salmonella; FISH; ELFA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000600006

Differential roles for pathogenicity islands SPI-13 and SPI-8 in the interaction of Salmonella Enteritidis and Salmonella Typhi with murine and human macrophages
Provedor de dados: 48
Autores: Espinoza,Rodrigo A.; Silva&amp;#8209;Valenzuela,Cecilia A.; Amaya,Fernando A.; Urrutia,Ítalo M.; Contreras,Inés; Santiviago,Carlos A.
Abstract  Background Salmonella pathogenicity island (SPI)-13 is conserved in many serovars of S. enterica, including S. Enteritidis, S. Typhimurium and S. Gallinarum. However, it is absent in typhoid serovars such as S. Typhi and Paratyphi A, which carry SPI-8 at the same genomic location. Because the interaction with macrophages is a critical step in Salmonella pathogenicity, in this study we investigated the role played by SPI-13 and SPI-8 in the interaction of S. Enteritidis and S. Typhi with cultured murine (RAW264.7) and human (THP-1) macrophages.  Results  Our results showed that SPI-13 was required for internalization of S. Enteritidis in murine but not human macrophages. On the other hand, SPI-8 was not required for the interaction of S. Typhi...
Tipo: Journal article
Palavras-chave: Salmonella; Enteritidis; Typhi; SPI-13; SPI-8; Macrophages; RAW264.7; THP-1.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100402

Resistance profile to antimicrobials of Salmonella spp. isolated from human infections
Provedor de dados: 52
Autores: Delicato,Elaine Regina; Mikcha,Jane Martha Graton; Fernandes,Sueli Aparecida; Pelayo,Jacinta Sanchez.
The purpose of this study was to analyse the profile of antimicrobial resistance among 21 strains of Salmonella isolated from patients with gastroenteritis symptom. It was observed that S. enteritidis was the serotype prevalent. These strains were sensitive to the majority of the antimicrobials tested, however, high resistance was observed in S. typhimurium and S. enterica subsp. enterica serotype 4,5,12:i:-. Surveillance and an efficient monitoring should be priority for the public health for the containment of antimicrobial resistance in foodborne infections.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Humans; Serotypes; Resistance; Antimicrobials.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200005

Estudo da ocorrência de salmonelas em ingredientes, rações e suabes de pó colhidos em uma fábrica industrial de ração
Provedor de dados: 62
Autores: ALBUQUERQUE,Ricardo; ITO,Nair Massako Katayama; MIYAJI,Claudio Issamu.
Durante um ano de colheita em uma fábrica comercial de ração, foram examinadas para a presença de Salmonella spp. 136 amostras de ingredientes de rações, 43 amostras de rações prontas e 110 amostras de suabes de pó. Apenas com o uso dos suabes é que não se conseguiu o isolamento. Entre os ingredientes examinados, encontrou-se salmonela em 19,85% das amostras, sendo que os subprodutos de origem animal foram mais fortemente contaminados, tendo propiciado 50% de percentual de isolamento; para os derivados lácteos, obtiveram-se 7,5%; e para outras matérias-primas, 12,5%. Foi obtido 4,65% de positividade para as rações examinadas.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Ração; Salmonella; Matérias-primas; Contaminação.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000600008

Salmonelose em cães de experimentação
Provedor de dados: 62
Autores: MEGID,Jane; ASSIS,Marcella Zampoli de; BRITO,Cristina de Jesus; LARA,Valéria Maria.
Relata-se um surto de salmonelose em cães de experimentação, com mortalidade de 50% nos animais. Os sorotipos isolados foram Salmonella Typhimurium, Salmonella Agona e Salmonella enterica subsp. Enterica. Discute-se a epidemiologia e sua importância em Saúde Pública.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Salmonelose animal; Salmonella; Cães.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962001000100009

Estudo comparativo de diferentes meios de cultura para o isolamento de salmonelas em matérias-primas e rações
Provedor de dados: 62
Autores: ALBUQUERQUE,Ricardo de; ITO,Nair Massako Kotayama; MIYAJI,Claudio Issamu.
A pesquisa foi conduzida com o objetivo de avaliar a eficiência de meios de isolamento de salmonelas. As matérias-primas e rações analisadas foram submetidas ao pré-enriquecimento em água peptonada tamponada, enriquecimento seletivo nos meios: Selenito cistina (SC), Tetrationato Hajna (TH) e Rappaport Vassiliadis (RV), e semeadura nos meios: Ágar Verde Brilhante (AVB) e Xilose Lisina Desoxicolato (XLD). Verificou-se que os caldos TH e RV produziram mais isolamentos que SC, que foi inferior nas condições deste trabalho, sendo que TH incubado a 42ºC produziu o maior número de resultados positivos, e os meios sólidos de plaqueamento AVB e XLD apresentaram eficiência semelhante.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Meios de cultura; Ração.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000100012

Sensitivity pattern of Salmonella serotypes in Northern India
Provedor de dados: 55
Autores: Gautam,Vikas; Gupta,Naveen Kumar; Chaudhary,Uma; Arora,D. R.
BACKGROUND: Enteric fever continues to be a major public health problem, especially in the developing countries of the tropics. We determined the incidence of Salmonella bloodstream infections and their antimicrobial resistance patterns from May to August in the years 1997-2001 in Haryana, a large state of India. The minimum inhibitory concentration (MIC) was also determined for 60 isolates of S. typhi to various commonly used antimicrobial agents. MATERIAL AND METHODS: Blood cultures of 6,956 patients (PUO/septicemia) were processed by standard procedures and the Salmonella spp. isolates were identified with specific antisera and with standard biochemical tests. Antimicrobial susceptibilities were determined by Stokes disc diffusion method. The MIC of 60...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Multidrug resistance.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000600003

Increasing prevalence and dissemination of invasive nontyphoidal Salmonella serotype Typhimurium with multidrug resistance in hospitalized patients from southern Brazil
Provedor de dados: 55
Autores: Reis,Rafael Oliveira dos; Souza,Margarida Neves; Cecconi,Maria Cristina Piccoli; Timm,Loeci; Ikuta,Nilo; Simon,Daniel; Wolf,Jonas Michel; Lunge,Vagner Ricardo.
ABSTRACT  Introduction: Nontyphoidal Salmonella serotypes are the main cause of human food-borne infection, including several hospitalization cases in the developing countries.  Aim: To detect the main serotypes and to characterize the antibiotic resistance of human non-enteric and enteric nontyphoidal Salmonella from clinical isolates in Brazil.  Methods: Salmonella serotypes were identified by microbiological and molecular methods. Susceptibility testing to antibiotics was performed by agar disk diffusion. Real-time PCRs were carried out for the detection of the genus Salmonella as well as serotypes Typhimurium and Enteritidis.  Results: A total of 307 nontyphoidal Salmonella were isolated from 289 different patients in a reference laboratory (LACEN-RS)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Antibiotic resistance; Typhimurium; Enteritidis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000500424

Contribution of different mechanisms to the resistance to fluoroquinolones in clinical isolates of Salmonella enterica
Provedor de dados: 55
Autores: Rushdy,Abeer Ahmed; Mabrouk,Mona Ibrahim; Abu-Sef,Ferialla Abdel-Hamid; Kheiralla,Zeinab Hassan; Abdel -All,Said Mohamed; Saleh,Neveen Mohamed.
OBJECTIVES: To study the potential factors include gene mutation, efflux pump and alteration of permeability associated with quinolone-resistance of Salmonella enterica strains isolated from patients with acute gastroenteritis and to evaluate the degree of synergistic activity of efflux pump inhibitors when combined with ciprofloxacin against resistant isolates. METHODS: Antimicrobial resistance patterns of fifty-eight Salmonella isolates were tested. Five isolates were selected to study the mechanism of resistance associated with quinolone group, including mutation in topoisomerase-encoding gene, altered cell permeability, and expression of an active efflux system. In addition, the combination between antibiotics and efflux pump inhibitors to overcome the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Fluoroquinolone resistance; Efflux pump; Outer membrane protein; Chromosomal mutations.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000400008

Prevalence and antimicrobial susceptibility of Salmonella serotypes in patients from Ribeirão Preto, São Paulo, Brazil, between 1985 and 1999
Provedor de dados: 55
Autores: Castro,Fabíola Attié de; Santos,Vanda Roseli dos; Martins,Carlos H. Gomes; Fernandes,Sueli A.; Zaia,José Eduardo; Martinez,Roberto.
Salmonella strains isolated from 1,138 samples representing 28,199 biological materials (stool, urine, blood and other fluids), collected between January 1985 and January 1999 at a reference University Hospital in Ribeirão Preto, São Paulo, Brazil, were studied. The most frequently detected serotypes were Salmonella enterica subspecies enterica serotype4,5,12:i:- (S. I 4,5,12:i:) (21.2%), S. agona (15.8%) and S. enteritidis (11.3%). A changing pattern of Salmonella serotypes was observed between 1985-1999. S. agona, which represented 27% of Salmonella serotypes isolated from 1985-1989, declined to 4% during the period from 1995 to 1999. S. enteritidis isolation remained below 1% until 1989; rose to 5.9% between 1990 and 1994, and increased to 32.3% between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Salmonellosis; Serotypes; Epidemiology and gastroenteritis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000500005

Mycotic aneurysm due to Salmonella species: clinical experiences and review of the literature
Provedor de dados: 56
Autores: Guo,Yiqun; Bai,Yu; Yang,Chunxia; Wang,Peng; Gu,Li.
The mortality of patients with mycotic aneurysms is high, especially in East Asia, and infection by Salmonella species is the most common. Our study aimed to improve prognosis of adult mycotic aneurysms with early diagnosis and accurate treatment. Four adult patients with mycotic aneurysm caused by Salmonella were included and analyzed by single-center retrospective analysis. Cases reported in the literature during the past 10 years were also summarized. The average age of the 4 male patients was 61.25 years, while that of the 53 cases reported in the literature was 65.13 years. Hypertension, diabetes, and atherosclerosis were common complications. Most patients presented fever and experienced pain at the corresponding position of the aneurysm. Laboratory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Mycotic aneurysms; EVAR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900201

Effect of the Escherichia coli EMO strain on experimental infection by Salmonella enterica serovar Typhimurium in gnotobiotic mice
Provedor de dados: 56
Autores: Lima-Filho,J.V.M.; Vieira,L.Q.; Arantes,R.M.E.; Nicoli,J.R.
An experimental infection with Salmonella enterica subsp. enterica serovar Typhimurium was evaluated in gnotobiotic mice previously exposed to a plasmid-free non-pathogenic Escherichia coli (EMO strain). Mice were exposed to EMO (experimental) or not (control) 10 days before challenge with Salmonella Typhimurium (10² colony forming units (CFU)/mouse). Survival after challenge was higher (P &lt; 0.05) in the experimental group (16%) than in the control animals (0%). Histopathological examination of the colon and ileum mucosa of the experimental group showed less extensive lesions such as edema, cell inflammatory infiltration and hyperemia. The epithelial cells of the mucosal surface and the production of the mucous layer were also better preserved in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Probiotic; Salmonella; Escherichia coli; Gnotobiotic mice.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700009

Plasmid-mediated quinolone resistance (PMQR) and mutations in the topoisomerase genes of Salmonella enterica strains from Brazil
Provedor de dados: 58
Autores: Ferrari,Rafaela; Galiana,Antonio; Cremades,Rosa; Rodríguez,Juan Carlos; Magnani,Marciane; Tognim,M.C.B.; Oliveira,Tereza C.R.M.; Royo,Gloria.
The objective of this study was to identify mutations in the Quinolone Resistance Determining sources Regions (QRDR) of the gyrA, gyrB, parC, and parE genes and to determine if any of the qnr variants or the aac(6')-Ib-cr variant were present in strains of Salmonella spp. isolated in Brazil. A total of 126 Salmonella spp. strains from epidemic (n = 114) and poultry (n = 12) origin were evaluated. One hundred and twelve strains (88.8%) were resistant to nalidixic acid (NAL) and 29 (23.01%) showed a reduced susceptibility to ciprofloxacin (Cip). The mutations identified were substitutions limited to the QRDR of the gyrA gene in the codons for Serine 83, Aspartate 87 and Alanine 131. The sensitivity to NAL seems to be a good phenotypic indication of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; PMQR; QRDR mutation; Quinolone; Ciprofloxacin.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200046

Antimicrobial activity of some of the south-Indian spices against serotypes of Escherichia coli, Salmonella, Listeria monocytogenes and Aeromonas hydrophila
Provedor de dados: 58
Autores: Indu,M.N.; Hatha,A.A.M.; Abirosh,C.; Harsha,U.; Vivekanandan,G.
Antibacterial activity of extracts of Allium sativum (garlic), Myristica fragrans (nutmeg), Zingiber officinale (ginger), Allium cepa (onion) and Piper nigrum (pepper) has been evaluated against 20 different serogroups of Escherichia coli, 8 serotypes of Salmonella, Listeria monocytogenes and Aeromonas hydrophila. Garlic extract showed excellent antibacterial activity against all the test organisms, except L. monocytogenes. Nutmeg showed good anti-listerial activity, although activity against E. coli and Salmonella were serotype dependent. Both garlic and nutmeg extracts were effective against A. hydrophila. Extracts of ginger showed inhibitory activity against two serogroups of E. coli: as O8 (enterotoxigenic E. coli) and O88 only. Extracts of onion and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spices extract; Antibacterial activity; Escherichia coli; Salmonella; Listeria monocytogenes; Aeromonas hydrophila.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200011

Distribution of virulence genes sefC, pefA and spvC in Salmonella Enteritidis phage type 4 strains isolated in Brazil
Provedor de dados: 58
Autores: Castilla,Karina Salvagni; Ferreira,Claudete Serrano Astolfi; Moreno,Andrea Micke; Nunes,Iolanda Aparecida; Ferreira,Antônio José Piantino.
The distribution of virulence genes, sefC, pefA and spvC, was investigated in 110 Salmonella Enteritidis phage type 4 strains by polymerase chain reaction. Their influence in the caecal colonization and invasion of liver and spleen of one-day-old chickens was studied. Eight isolates were negative for the spvC gene, three for the pefA gene and one, for the sefC gene. These results allowed grouping the strains into four genotypes. Presence of these genes did not influence bacteria invasion in the liver and spleen of the chickens ten days after infection, although the presence of more than one fimbrial gene can be related to caecal colonization.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Virulence genes; Colonization; Invasion.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200007

Requirement for cobalamin by Salmonella enterica serovars Typhimurium, Pullorum, Gallinarum and Enteritidis during infection in chickens
Provedor de dados: 58
Autores: Paiva,Jacqueline Boldrin de; Penha Filho,Rafael Antonio Casarin; Berchieri Junior,Angelo; Lemos,Manoel Victor Franco.
Salmonella enterica serovar Typhimurium synthesizes cobalamin (vitamin B12) only during anaerobiosis. Two percent of the S. Typhimurium genome is devoted to the synthesis and uptake of vitamin B12 and to B12-dependent reactions. To understand the requirement for cobalamin synthesis better, we constructed mutants of Salmonella serovars Enteritidis and Pullorum that are double-defective in cobalamin biosynthesis (ΔcobSΔcbiA). We compared the virulence of these mutants to that of their respective wild type strains and found no impairment in their ability to cause disease in chickens. We then assessed B12 production in these mutants and their respective wild type strains, as well as in S. Typhimurium ΔcobSΔcbiA, Salmonella Gallinarum ΔcobSΔcbiA, and their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Cobalamin; Gene deletion; Chickens.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400024

Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts
Provedor de dados: 58
Autores: Ribeiro,Aldemir Reginato; Nascimento,Vladimir Pinheiro do; Cardoso,Martha Oliveira; Santos,Luciana Ruschel dos; Rocha,Silvio Luís da Silveira.
This study was conducted aiming to compare the conventional microbiological method to detect Salmonella in broiler parts with the Immunomagnetic Separation method (IMS) followed by plate isolation and also the IMS associated with Rappaport-Vassiliadis broth (RV). The IMS was performed following a pre- enrichment step in buffered peptone water. Sixty-one samples (raw broiler parts) were tested and the results showed that the use of the IMS method alone allowed the isolation of Salmonella in 9 of the tested samples, while the association IMS/RV detected the agent in 30 samples. The conventional microbiological method was able to isolate the agent in 25 opportunities. These results allowed to conclude that the IMS/RV association presented an increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunomagnetic separation; Salmonella; Raw broiler parts.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400012

Salmonella spp. in raw broiler parts: occurrence, antimicrobial resistance profile and phage typing of the Salmonella Enteritidis isolates
Provedor de dados: 58
Autores: Ribeiro,Aldemir Reginato; Kellermann,Aline; Santos,Luciana Ruschel dos; Bessa,Marjo Cadó; Nascimento,Vladimir Pinheiro do.
The present study was carried out to evaluate the occurrence of Salmonellae in raw broiler parts and to determine the antimicrobial resistance profile of the isolated strains. Twenty-four (39.3%) broiler parts samples were positive for Salmonella and twenty-five Salmonella strains were isolated, since two different serovars were detected in one single positive sample. Salmonella Enteritidis was the most prevalent serovar. Among Salmonella Enteritidis isolates, 95.2% belonged to Phage Type 4 (PT4) (20/21) and 4.8% to PT7 (1/21). Twenty-two (88%) strains of Salmonella were resistant to at least one antimicrobial agent, generating eight different resistance patterns. The S. Typhimurium (n: 1) and S. Hadar (n: 3) isolates presented multiple resistance. Three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Broiler parts; Antimicrobial resistance; Phage typing.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200021

Isolation of Escherichia coli and Salmonella spp. from free-ranging wild animals
Provedor de dados: 58
Autores: Iovine,Renata de Oliveira; Dejuste,Catia; Miranda,Flávia; Filoni,Claudia; Bueno,Marina Galvão; Carvalho,Vania Maria de.
Increasing interactions between humans, domestic animals and wildlife may result in inter-species transmission of infectious agents. To evaluate the presence of pathogenic E. coli and Salmonella spp. and to test the antimicrobial susceptibility of isolates, rectal swabs from 36 different free-ranging wild mammals were taken from two distinct natural sites in Brazil: Cantareira State Park (CSP, state of São Paulo) and Santa Isabel do Rio Negro Region (SIRNR, state of Amazonas). The swabs were randomly collected and processed for bacterial isolation, identification, characterization and antimicrobial resistance. Eighteen E. coli strains from CSP and 20 from SIRNR were recovered from 14 and 22 individuals, respectively. Strains from animals captured in CSP,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: E. coli; Salmonella; Wild mammals; One Health; Brazil.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401257

Evaluation of three enrichment broths and five plating media for Salmonella detection in poultry
Provedor de dados: 58
Autores: Rall,Vera Lúcia Mores; Rall,Ricardo; Aragon,Lina Casale; Silva,Márcia Guimarães da.
We evaluated the effectiveness of Selenite Cystine (SC), Tetrathionate Brilliant Green (TBG) and Rappaport Vassiliadis (RV) broths for Salmonella isolation. We also tested three classic plating media, Salmonella-Shigella Agar (SS), Brilliant Green Agar (BGA), Xylose Lysine Desoxycholate Agar (XLD) and two chromogenic agars, Rambach (RA) and CHROMagar Salmonella (CAS). Among 100 poultry carcasses, 29 were positive for Salmonella using all plating media combined. RV broth (69%) and TT broth (58.6%) were more effective than SC broth (24.1%). The chromogenic media gave better results than the classic ones with less false-positive colonies. The most effective isolation medium was CHROMagar, where Salmonella was identified in 23 (79.3%) of the 29 positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Poultry; Plating media; Selective enrichment.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200009

Phage amplification assay as rapid method for Salmonella detection
Provedor de dados: 58
Autores: Siqueira,Regina Silva de; Dodd,Christine E.R.; Rees,Catherine E.D.
The application of rapid methods is crucial for the HACCP program implantation in food industry. In this context, Phage Amplification Assay is a good candidate because is based on the interactions of phage and their host bacteria. This method using phage P22 was applied with to detect Salmonella cells in chicken breast. Samples of 25 g of chicken breast were diluted and the appropriate dilutions were used in phage amplification assay for Salmonella detection. After 3-4 h of incubation, it was observed a phage titre of approximately 10(4) pfu mL-1, indicating that there were Salmonella cells which were naturally present in the meat. The presence of Salmonella cells were verified by using direct plating on XLD agar and by conventional enrichment procedure....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Rapid method; Bacteriophage; P22.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500040

Salmonella spp investigation in transport boxes of day-old birds
Provedor de dados: 58
Autores: Zancan,Fábio Tavares; Berchieri Junior,Angelo; Fernandes,Sueli Aparecida; Gama,Nilce Maria Soares Queiroz.
The presence of Salmonella was investigated in transport boxes of newly hatched chicks in the moment they were received in the farms. 1,611 boxes, delivered to 15 farms (one broiler grand-parent farm, four broiler parent farms, two commercial broiler chick farms and seven laying hens farms) were tested. A total of 19 visits to these farms were done, and Salmonella was detected in ten of these visits. Salmonella Heidelberg was detected in boxes in five farms. S. Heidelberg and S. Mbandaka were simultaneously detected in one broiler breeder farm. Salmonella Enteritidis alone, S. Enteritidis and S. Mbandaka and S. Cerro alone were detected in three, one and one laying hens farms, respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Day-old bird; Transport box.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300016

Assessment of the risks of rats (Rattus norvegicus) and opossums (Didelphis albiventris) in different poultry-rearing areas in Argentina
Provedor de dados: 58
Autores: Villafañe,Isabel E. Gómez; Miñarro,Fernando; Ribicich,Mabel; Rossetti,Carlos A.; Rossotti,Daniel; Busch,María.
We have studied the prevalence of Trichinella spiralis, Leptospira spp. and Salmonella spp. in rats and opossums that inhabit poultry farms of Exaltación de la Cruz, Buenos Aires, Argentina, to determine the potential sanitary risk for humans that are in contact with these animals. The study was carried out on 48 poultry farms between spring 1999 and winter 2001. The study of opossums began in winter 2000. During the study period we captured 152 Rattus norvegicus, 3 Rattus rattus, 16 Didelphis albiventris and 1 Lutreolina crassicaudata. We have registered the presence of rats and opossums in 70% and 27% of the studied farms, respectively. The percentage of farms with rats was independent of the presence or absence of pigs. We did not detect the presence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zoonosis; Salmonella; Poultry farms; Rats; Opossum.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300017

Microbiological quality of organic vegetables produced in soil treated with different types of manure and mineral fertilizer
Provedor de dados: 58
Autores: Machado,Débora Cabral; Maia,Carla Marques; Carvalho,Isabel Dias; Silva,Natan Fontoura da; André,Maria Cláudia Dantas Porfírio Borges; Serafini,Álvaro Bisol.
An attempt was made to evaluate microbiological quality of horticultural crops grown organically. Three species of vegetables were used, lettuce (Lactuva sativa), radish (Raphanus sativus) and spinach (Tetragonia expansa), grown organically, in fertile soil. Six different treatments were applied: mineral fertilizer, chicken, cow, and pig manure, chicken litter and cow manure, in association with a liquid foliar biofertilizer. These crops were handled according to correct agronomic practices for growing crops organically for commercial purposes. Samples were examined for the Most Probable Number (MPN/g/mL) of total and fecal coliforms and to detect the presence of Escherichia coli and Salmonella spp. All analyzed samples were considered acceptable for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Organic vegetables; Animal manure; Coliforms; Escherichia coli; Salmonella.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400025

Rapid detection of food-borne Salmonella contamination using IMBs-qPCR method based on pagC gene
Provedor de dados: 58
Autores: Wang,Jiashun; Li,Yi; Chen,Jia; Hua,Deping; Li,Yi; Deng,Hui; Li,Ying; Liang,Zhixuan; Huang,Jinhai.
Abstract Detection of Salmonella is very important to minimize the food safety risk. In this study, the recombinant PagC protein and PagC antibody were prepared and coupled with immunomagnetic beads (IMBs) to capture Salmonella cells from pork and milk samples. And then the SYBR Green qualitative PCR was developed to detect the pathogenic Salmonella. The results showed that the PagC polyclonal antiserum is of good specificity and the capture rate of 0.1 mg IMBs for Salmonella tended to be stable at the range of 70-74% corresponding to the concentrations between 101 and 104 CFU/mL. The method developed demonstrated high specificity for the positive Salmonella samples when compared to non-specific DNA samples, such as Escherichia coli, Staphylococcus aureus,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; IMBs-qPCR; PagC gene; Detection.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200320

Characterization of class 1 integrons and antibiotic resistance genes in multidrug-resistant Salmonella enterica isolates from foodstuff and related sources
Provedor de dados: 58
Autores: Ribeiro,Vinicius B.; Lincopan,Nilton; Landgraf,Mariza; Franco,Bernadete D.G.M.; Destro,Maria T.
In recent years, an increase in the occurrence of antimicrobial resistance among Salmonella enterica has been observed in several countries, which is worrisome because S. enterica is one of the most common causes of human gastroenteritis worldwide. The aim of this study was to characterize class 1 integrons and antibiotic resistance genotypes in Salmonella enterica isolates recovered from foodstuff and related sources. Nineteen multidrug-resistant (MDR) Salmonella enterica isolates were recovered. Higher resistance rates to tetracycline (90%), streptomycin (80%), sulfamethoxazole-trimethoprim (80%), ampicillin (60%) and nalidixic acid (70%) were related to the presence of the tetA, aadA, sul1/sul2, blaTEM-1 genes, and a codon mutation at position 83 of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Multidrug-resistant; Class 1 integrons; Foodstuff; Brazil.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200033

A novel multiplex PCR for the simultaneous detection of Salmonella enterica and Shigella species
Provedor de dados: 58
Autores: Radhika,M.; Saugata,Majumder; Murali,H.S.; Batra,H.V.
Salmonella enterica and Shigella species are commonly associated with food and water borne infections leading to gastrointestinal diseases. The present work was undertaken to develop a sensitive and reliable PCR based detection system for simultaneous detection of Salmonella enterica and Shigella at species level. For this the conserved regions of specific genes namely ipaH1, ipaH, wbgZ, wzy and invA were targeted for detection of Shigella genus, S. flexneri, S. sonnei, S. boydii and Salmonella enterica respectively along with an internal amplification control (IAC). The results showed that twenty Salmonella and eleven Shigella spp., were accurately identified by the assay without showing non-specificity against closely related other Enterobacteriaceae...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Shigella; Multiplex PCR; Spiking.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200041

Production and caracterization of monoclonal antibodies for the detection of Salmonella enterica in chicken meat
Provedor de dados: 58
Autores: Schneid,Andréa dos Santos; Lüdtke,Charli Beatriz; Diel,Cristiane; Aleixo,José AntonioGuimarães.
A panel of 13 monoclonal antibodies (MAbs) that react against outer membrane proteins of Salmonella Enteritidis was obtained. Two MAbs were classified as IgM, six were IgG2a, three were IgG3 and one was of the IgG2b isotype. The reactivity of the MAbs against different serovars of Salmonella enterica and other bacteria was investigated using an indirect ELISA. Five MAbs reacted only against Salmonella Enteritidis. Two MAbs presented crossed reactions with thermo-extracted antigens of Klebsiella pneumoniae, Citrobacter freundii and Enterobacter aerogenes. MAb 424H presented wide spectrum of reactivity, detecting antigens of Salmonella belonging to serogroups B, C, D, E and G. The detection limit of different serovars of Salmonella in a indirect ELISA with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Monoclonal antibodies; Membrane proteins; Foods.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200012

Comparision of the BAX® System with an in-house MSRV method for the detection of Salmonella in chicken carcasses and pork meat
Provedor de dados: 58
Autores: Franchin,Paulo R.; Ogliari,Paulo J.; Andrade,Dalton F.; Chiapinoto,Maura; Lemos,Giovana; Rebelatto,Marina; Silva,Ivair G. da; Batista,Cleide R.V.
A study was performed to compare the analytical procedure of the BAX® System for Salmonella PCR assay with the Modified Semi-Solid Rappaport-Vassiliadis (MSRV) method, for the detection of Salmonella in naturally contaminated chicken carcass samples (n = 762) and raw pork meat (n = 566). The chicken carcasses samples were collected during slaughtering after defeathering or immediately after evisceration and the raw pork meat collected from the deboned head of recently slaughtered pigs and others deboned raw fresh pork meat. The BAX® System detected 134 Salmonella-positive samples in chicken carcasses and 145 samples in pork meat, while the MSRV method isolated 142 and 144 Salmonella-positive samples, respectively. No significant difference was observed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BAX® System; MSRV method; Salmonella; Chicken; Pork meat.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400022

Detection of salmonella sp in chicken cuts using immunomagnetic separation
Provedor de dados: 58
Autores: Conceição,Rita de Cássia dos Santos da; Moreira,Ângela Nunes; Ramos,Roberta Juliano; Goularte,Fabiana Lemos; Carvalhal,José Beiro; Aleixo,José Antonio Guimarães.
The immunomagnetic separation (IMS) is a technique that has been used to increase sensitivity and specificity and to decrease the time required for detection of Salmonella in foods through different methodologies. In this work we report on the development of a method for detection of Salmonella in chicken cuts using in house antibody-sensitized microspheres associated to conventional plating in selective agar (IMS-plating). First, protein A-coated microspheres were sensitized with polyclonal antibodies against lipopolysacharide and flagella from salmonellae and used to standardize a procedure for capturing Salmonella Enteritidis from pure cultures and detection in selective agar. Subsequently, samples of chicken meat experimentally contaminated with S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Immunomagnetic separation; Rapid methods.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100034

Prevention of Salmonella infection by contact using intestinal flora of adult birds and/or a mixture of organic acids
Provedor de dados: 58
Autores: Oliveira,Gláucia Helaine de; Berchieri Junior,Angelo; Barrow,Paul A.
This study was carried out to assess the ability of competitive exclusion and a mixture of organic acids to prevent Salmonella infection by contact between newly hatched chicks. A bird infected with Salmonella was placed in a box containing non-infected birds, previously treated with a broth culture of faeces of adult birds (CE) and/or a mixture of organic acids. The number of Salmonella organisms in the caeca of the contact birds was estimated at 4 and 8 days post-challenge. The birds were infected with Salmonella Typhimurium, Salmonella Enteritidis (both repeated 5 times), Salmonella Agona and Salmonella Infantis (3 repetitions). The same approach was used to test the mixture of organic acids alone. In this case the birds received feed containing 0.8% of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Competitive exclusion; Organic acids; By-contact infection; Poultry.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200011

Hazards in non-pasteurized milk on retail sale in Brazil: prevalence of Salmonella spp, Listeria monocytogenes and chemical residues
Provedor de dados: 58
Autores: Nero,Luís Augusto; Mattos,Marcos Rodrigues de; Beloti,Vanerli; Barros,Marcia A.F.; Netto,Daisy Pontes; Pinto,José Paes A.N.; Andrade,Nélio José de; Silva,Wladimir P.; Franco,Bernadette D.G.M.
Fluctuations in the Brazilian milk market force small milk producers to find temporary trade alternatives, which include selling raw milk to people who prefer this type of milk rather than heat-processed milk. Considering the importance of these small milk producers to the market and the well-known health risks associated to consumption of raw milk, this study evaluated the microbiological quality and the presence of Listeria monocytogenes, Salmonella spp., chlorine, antimicrobials and insecticides (organophosphates and carbamates) in raw milk produced in 210 small and medium farms located in four important milk-producing Brazilian states (Paraná, São Paulo, Minas Gerais and Rio Grande do Sul). In 66% of the selected farms the milking was manual. In 33% of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hazards; Raw milk; Salmonella; Listeria monocytogenes; Hygiene indicators; Insecticides; Antimicrobials.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200007

Mannanoligosaccharide agglutination by Salmonella enterica strains isolated from carrier pigs
Provedor de dados: 58
Autores: Borowsky,Luciane; Corção,Gertrudes; Cardoso,Marisa.
Type-1 fimbriae are associated with most Salmonella enterica serovars and are an essential factor for host colonization. Mannanoligosaccharides (MOS), a prebiotic that is agglutinated by type-1 fimbriae, are proposed for the control of enterobacteria colonization and may be an alternative to Salmonella control in pigs. The aim of this study was to evaluate the capability of porcine Salmonella strains to adhere to MOS in vitro. A total of 108 strains of Salmonella sp. isolated from carrier pigs were evaluated for the amplification of fimA and fimH genes, agglutination of MOS and hemagglutination. In all tested strains, amplicons of expected size were detected for both fimA and fimH gene. In the hemagglutination assays, 31 (28.7%) strains presented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Type-1 fimbriae; Mannanoligosaccharides.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300007

Salmonella in Brazilian and imported pet reptiles
Provedor de dados: 58
Autores: Sá,Isabel Valéria Abalem de; Solari,Claude André.
The presence of salmonellae in fecal samples or cloacal swabs of 97 pet reptiles (15 snakes, 24 lizards and 58 chelonians) was investigated. Thirty seven animals had national origin and 60 were imported. Salmonella spp was detected in 39.1% of the reptiles, being 62.5% in lizards, 53.3% in snakes and 25.8% in chelonians. Strains belonged to subspecies I (44.7%), II (10.5%), IIIa (5.2%), IIIb (21.0%) and IV (18.5%) of the enterica species, with predominance (55.3%) of subspecies usually found in cold-blooded animals (II to IV). In the subspecies I, the serovars Albany, Enteritidis and Typhimurium predominated. The Trachemys scripta elegans imported turtles corresponded to 93.3% (14/15) of the salmonellae-positive chelonians. The national iguanas presented a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Pet reptiles.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400007

Prevalence, serotyping and antimicrobials resistance mechanism of Salmonella enterica isolated from clinical and environmental samples in Saudi Arabia
Provedor de dados: 58
Autores: El-Tayeb,Mohamed A.; Ibrahim,Abdelnasser S.S.; Al-Salamah,Ali A.; Almaary,Khalid S.; Elbadawi,Yahya B.
Abstract Salmonella is recognized as a common foodborne pathogen, causing major health problems in Saudi Arabia. Herein, we report epidemiology, antimicrobial susceptibility and the genetic basis of resistance among S. enterica strains isolated in Saudi Arabia. Isolation of Salmonella spp. from clinical and environmental samples resulted in isolation of 33 strains identified as S. enterica based on their biochemical characteristics and 16S-rDNA sequences. S. enterica serovar Enteritidis showed highest prevalence (39.4%), followed by S. Paratyphi (21.2%), S. Typhimurium (15.2%), S. Typhi and S. Arizona (12.1%), respectively. Most isolates were resistant to 1st and 2nd generation cephalosporin; and aminoglycosides. Moreover, several S. enterica isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Serotyping; Antibiotic resistance; Antimicrobials resistance determinants.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300499

Detection of Salmonella sp. from porcine origin: a comparison between a PCR method and standard microbiological techniques
Provedor de dados: 58
Autores: Castagna,Sandra Maria Ferraz; Muller,Monika; Macagnan,Marisa; Rodenbusch,Carla Rosane; Canal,Cláudio Wageck; Cardoso,Marisa.
The aim of this study was to compare a polymerase chain reaction (PCR) method combined with selective enrichment in Rappaport-Vassiliadis broth (PCR-RVB) with standard microbiological techniques (SMT) for the generic detection of Salmonella in samples of porcine origin. Two hundred sixty eight field samples consisting of 42 sets of pooled porcine mandibular lymph nodes and tonsils, 44 samples of intestinal content, 38 pork sausage meat samples and 144 samples of feed collected from swine farms were submitted to the PCR-RVB and SMT protocols. Salmonella was detected in 54 samples using the PCR-RVB assay and in 42 samples by SMT, three of the SMT Salmonella-positive samples (one each of S. Derby, S. Panama and S. Typhimurium) being Salmonella-negative by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Detection; PCR; Pork; Salmonella; Swine.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400013

Characterization of antibiotic resistance in Salmonella enterica isolates determined from ready-to-eat (RTE) salad vegetables
Provedor de dados: 58
Autores: Taban,Birce Mercanoglu; Aytac,Sait Aykut; Akkoc,Nefise; Akcelik,Mustafa.
In the last decade, ready-to-eat (RTE) salad vegetables are gaining increasing importance in human diet. However, since they are consumed fresh, inadequate washing during processing can bring on some foodborne illnesses, like salmonellosis, since these food items have natural contamination from soil and water. During 2009-2010, a total of 81 samples were purchased arbitrarily from local markets in Ankara, and were examined for Salmonella contamination. Salmonella screening was performed by using anti-Salmonella magnetic beads system and polymerase chain reaction (PCR) identification of the suspected colonies. Then, the antibiotic resistance profiles of four Salmonella strains identified (strains RTE-1, RTE-2, RTE-3, and RTE-4) were also investigated, since...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Contamination; Plasmid-mediated sulfonamide resistance; Ready-to-eat salad vegetables; Food safety; Salmonella.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200007

Cytotoxic T cell adjuvant effects of three Salmonella enterica flagellins
Provedor de dados: 58
Autores: Braga,Catarina J.M.; Massis,Liliana M.; Alencar,Bruna C.G.; Rodrigues,Maurício M.; Sbrogio-Almeida,M.E.; Ferreira,Luís C.S.
Bacterial flagellins are important virulence-associated factors and strong inducers of inflammatory responses in mammalian hosts. Flagellins have also been investigated as potential vaccine adjuvants, either for induction of humoral or cellular immune responses, to different target antigens. In this study we investigated the adjuvant properties of three Salmonella enterica flagellins types (FliCd, FliCi and FljB) to an ovalbumin-derived CD8+ T cell-restricted epitope (OVA257264). Although mice immunized with the three tested flagellins elicited antigen-specific activated CD8+ T cells, only animals immunized with FliCi and FliCd flagellins admixed with ovalbumin mounted specific in vivo cytotoxic responses to peptide-pulsed target cells. The present results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Flagellin; Cytotoxic T cells; Adjuvants; Vaccines.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100011

Microbial contamination of sand from major beaches in Fortaleza, Ceará State, Brazil
Provedor de dados: 58
Autores: Vieira,Regine Helena Silva dos Fernandes; Rodrigues,Dália dos Prazeres; Menezes,Everardo A.; Evangelista,Norma Suely de Santana; Reis,Eliane M.F. dos; Barreto,Leopoldo Melo; Gonçalves,Flávia Araújo.
The presence of faecal contamination and pathogenic microorganisms in samples of dry and wet sand collected from three major beaches in Fortaleza, Ceará State, Brazil: (Praia do Mucuripe, Praia do Futuro and Praia do Caça e Pesca), during the period of May 1999 to January 2000 was evaluated. Praia do Caça e Pesca had the highest incidence of E. coli in dry sand (56%) followed by Praia do Mucuripe (28%) and Praia do Futuro (16%). In wet sand, results were 48%, 28% and 24% for Praia do Caça e Pesca, Praia do Futuro and Praia do Mucuripe, respectively. Only two samples from Praia do Futuro, one from dry sand and another one from wet sand, were positive for Salmonella. V. parahaemolyticus was isolated from four samples from Praia do Caça e Pesca (two from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sand contamination; Yeasts; Faecal coliforms; Vibrio parahaemolyticus; Salmonella.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200001

Biocide tolerance in Salmonella from meats in Southern Spain
Provedor de dados: 58
Autores: Garrido,Antonio Marin; Burgos,Mª Jose Grande; Márquez,Mª Luisa Fernández; Aguayo,Mª Carmen López; Pulido,Rubén Pérez; Árbol,Julia Toledo del; Gálvez,Antonio; López,Rosario Lucas.
Salmonella serovars sampled from meat products in Southern Spain (Andalucía) during the period 2002-2007 were analyzed in this study. The serovars most frequently detected (in order) were Typhimurium, Enteritidis, Derby, Anatum and Rissen. Isolates (n = 43) were tested for sensitivity to biocides, including the quaternary ammonium compounds benzalkonium chloride (BC), cetrimide (CT) and hexadecylpyridinium chloride (HDP), and the bisphenols triclosan (TC) and hexachlorophene (CF). The minimum inhibitory concentration (MIC) for the quaternary ammonium compounds was in the range of 25 to 50 mg/L for most isolates, although a few isolates required much higher concentrations, up to 250 mg/L. Bisphenols showed higher inhibitory activity, with a MIC of 2.5 to 25...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Biocides; Serovars; Meat; Chorizo.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401177

Protection by Lactobacillus acidophilus UFV-H2B20 against experimental oral infection with Salmonella enterica subsp. enterica Ser. Typhimurium in gnotobiotic and conventional mice
Provedor de dados: 58
Autores: Moura,Lilian Nobre; Neumann,Elizabeth; Vieira,Leda Quercia; Nicoli,Jacques R.
The ability of Lactobacillus acidophilus UFV-H2B20 to antagonize Salmonella enterica subsp. enterica ser. Typhimurium and to reduce the pathological consequences for the host was determining using conventional and gnotobiotic animals. Conventional NIH mice received daily by gavage a 0.1 ml suspension containing about 10(8) cfu L. acidophilus UFV-H2B20 and germfree animals received a single 0.1 ml dose. The gnotobiotic and conventional groups were infected orally with 10² and 10(5) cfu of S. Typhimurium, respectively, 7 days after the beginning of treatment. Control groups were treated with sterile saline instead of Lactobacillus. Survival data showed a protective effect against the pathogenic bacteria in both conventional and gnotobiotic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus; Salmonella; Probiotic; Gnotobiotic mice.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100015

Enterobacteriaceae in mouth and cloaca of podocnemis expansa and P. Unifilis (testudines: chelonia) populations of national park of araguaia plains, Brazil
Provedor de dados: 58
Autores: Morais,Paula Benevides de; Souza,Denise Rodrigues de; Sousa,Francisca Maria Pinheiro de; Oliveira,Kleverson Wessel de; Pimenta,Raphael Sanzio.
Shigella flexnerii and Escherichia coli were the most frequent Gram-negative bacteria found in the mouth cavity and cloacae of the turtles Podocnemis expansa and P. unifilis on beaches in the National Park of Araguaia, Brazil. Reptiles are known as Salmonella carriers, despite rarely isolated in these turtles.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Shigella; Enterobacteriaceae; Turtles; Bananal Island.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200017

Survival of Salmonella spp. in minced meat packaged under vacuum and modified atmosphere
Provedor de dados: 58
Autores: Djordjević,Jasna; Bošković,Marija; Starčević,Marija; Ivanović,Jelena; Karabasil,Nedjeljko; Dimitrijević,Mirjana; Lazić,Ivana Branković; Baltić,Milan Ž.
Abstract The effect of different modified atmosphere packaging regimes on the behavior of Salmonella spp. on minced meat was studied. Minced meat was experimentally contaminated with a Salmonella spp. cocktail (S. Enteritidis, S. Typhimurium, S. Infantis and S. Arizonae), packaged under vacuum or modified atmosphere with initial headspaces containing 20%O2/50%CO2/30%N2 and 20%O2/30%CO2/50%N2) and stored at 3 ± 1 °C for 12 days. Samples were analyzed for Salmonella spp., viable and lactic acid bacteria count every third day. Salmonella spp. counts decreased during storage in all packaging types, with reductions of about 1.5 log CFU/g. A significant difference (p &lt; 0.01) was noted between Salmonella spp. counts in meat packaged in vacuum and modified...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Minced meat; Modified atmosphere packaging; Vacuum; Salmonella; PH.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300607

Antimicrobial effect of copper surfaces on bacteria isolated from poultry meat
Provedor de dados: 58
Autores: Parra,Angel; Toro,Magaly; Jacob,Ricardo; Navarrete,Paola; Troncoso,Miriam; Figueroa,Guillermo; Reyes-Jara,Angélica.
Abstract Poultry meat is a food product that usually carries high rates of microbial contamination, including foodborne pathogens. The poultry industry has established different systems to minimize these hazards. In recent years, extensive literature has demonstrated the antimicrobial activity of different contact surfaces made of copper to effectively reduce microbial loads. The aim of the present study was to evaluate the antibacterial effect of copper surfaces on the transmission of two foodborne pathogens – Salmonella enterica and Listeria monocytogenes – and a poultry native microbiota bacterial species – Enterobacter cloacae. We also evaluated the impact of the poultry meat matrix on the antimicrobial activity of a copper surface. Our results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial copper-alloys; Foodborne pathogens; Salmonella; Listeria monocytogenes; Poultry meat.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500113

Occurrence of Salmonella spp. in broiler chicken carcasses and their susceptibility to antimicrobial agents
Provedor de dados: 58
Autores: Duarte,Dalila Angélica Moliterno; Ribeiro,Aldemir Reginato; Vasconcelos,Ana Mércia Mendes; Santos,Sylnei Barros; Silva,Juliana Vital Domingos; Andrade,Patrícia Lúcia Arruda de; Falcão,Lúcia Sadae Pereira da Costa de Arruda.
The present study was carried out to evaluate the occurrence of Salmonellae in broiler chicken carcasses and to determine the antimicrobial resistance profile of the isolated strains. Twenty-five out of the 260 broiler chicken carcasses samples (9.6%) were positive for Salmonella. S. Enteritidis was the most frequent serovar. Nineteen Salmonella isolates were tested for antimicrobial resistance, and the results indicated that 94.7% were resistant to at least one antimicrobial agent. Resistance to streptomycin (73.7%), nitrofurantoin (52.3%), tetracycline (31.6%), and nalidixic acid (21%) were the prevalent amongst Salmonella strains tested.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Broiler chicken carcasses; Antimicrobial resistance; Salmonella.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300020

Occurrence of Salmonella sp. and coagulase-positive staphylococci in raw eggs and Coalho cheese: comparative study between two cities of Brazil's northeast
Provedor de dados: 58
Autores: Evêncio-Luz,L; Lima-Filho,J.V.; Evêncio-Neto,J.
Microbiological analyses of chicken eggs in Recife and Salvador have shown a high occurrence of Salmonella in the egg shells and yolks. Likewise, the occurrence of Salmonella plus coagulase-positive staphylococci in Coalho cheese reached alarming levels. The data revealed a significant risk of infections and intoxications from consuming these foods in the cities.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Staphylococcus; Coalho cheese; Eggs.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400030

Quantification and molecular characterization of Salmonella isolated from food samples involved in salmonellosis outbreaks in Rio Grande do Sul, Brazil
Provedor de dados: 58
Autores: Mürmann,Lisandra; Santos,Maria Cecília dos; Longaray,Solange Mendes; Both,Jane Mari Corrêa; Cardoso,Marisa.
Data concerning the prevalence and populations of Salmonella in foods implicated in outbreaks may be important to the development of quantitative microbial risk assessments of individual food products. In this sense, the objective of the present study was to assess the amount of Salmonella sp. in different foods implicated in foodborne outbreaks in Rio Grande do Sul occurred in 2005 and to characterize the isolated strains using phenotypic and genotypic methods. Nineteen food samples involved in ten foodborne outbreaks occurred in 2005, and positive on Salmonella isolation at the Central Laboratory of the Health Department of the State of Rio Grande do Sul, were included in this study. Food samples were submitted to estimation of Salmonella using the Most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Foodborne outbreak; Quantification; PFGE.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300024

Adhesion and biocides inactivation of Salmonella on stainless steel and polyethylene
Provedor de dados: 58
Autores: Tondo,Eduardo Cesar; Machado,Taís Raquel Marcon; Malheiros,Patrícia da Silva; Padrão,Débora Kruger; Carvalho,Ana Lyl de; Brandelli,Adriano.
The adhesion of Salmonella (S.) strains to stainless steel and polyethylene and their inactivation by biocides used in food industry was investigated. Coupons of stainless steel and polyethylene were immersed in bacterial suspensions of S. Enteritidis, S. Typhimurium, and S. Bredeney during 15, 30, and 60 minutes, and submitted to different concentrations of peracetic acid (PAA), sodium hypochlorite (NaOCl), and quaternary ammonium (Quat) sanitizers. Hydrophobicity of the surfaces was evaluated by contact angle measurements using the sessile drop method and bacterial adhesion was accompanied through bacterial counts and scanning electron microscopy (SEM). Results indicated that the three serovars of Salmonella presented similar adhesion to both materials...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Adhesion; Biocides; Stainless steel; Polyethylene.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400022

Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat
Provedor de dados: 58
Autores: Schneid,Andréa dos Santos; Rodrigues,Kelly Lameiro; Chemello,Davi; Tondo,Eduardo Cesar; Ayub,Marco Antônio Zacchia; Aleixo,José Antonio Guimarães.
In this work, an indirect ELISA based on a monoclonal antibody (MAb) specific for an outer membrane protein of Salmonellaenterica serovar Enteritidis was used for detection of Salmonella in 154 samples of chicken meat. Its efficiency was determined through comparison with the results obtained from the conventional method. The prevalence of samples contaminated with Salmonella was 23% with the conventional culture method, and 26% with the ELISA. From thirty-five samples positive for Salmonella by the conventional method, 33 were also positive by ELISA. Seven other samples were only positive in the ELISA. Comparison of the results obtained in the two methods showed an ELISA sensitivity and specificity of 94%, and positive and negative predictive values of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Monoclonal antibody; Policlonal antibody; ELISA.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300027

Frequency of zoonotic bacteria among illegally traded wild birds in Rio de Janeiro
Provedor de dados: 58
Autores: Matias,Carlos Alexandre Rey; Pereira,Ingrid Annes; Reis,Eliane Moura Falavina dos; Rodrigues,Dália dos Prazeres; Siciliano,Salvatore.
Abstract The illegal wildlife trade may increase the risk of infectious disease transmission, and it may not only cause disease outbreaks in humans but also threaten livestock, native wild populations, and ecosystems' health. Bird species may act as carriers in the transmission of enteric pathogens. However, epidemiological studies on zoonotic bacteria in wild birds are rare in Brazil. From March 2011 to March 2012, we investigated the frequency of Enterobacteriaceae in cloacal swab samples from 109 birds of the passerine and Psittacidae families. These birds were recovered from illegal trade in Rio de Janeiro, Brazil, and sent to a rehabilitation center. Gram-negative bacteria were isolated from 86 wild birds (78.9%). A mean (±SD) of 1.68 (±1.30)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotic resistance; Enterobacteriaceae; Public health; Salmonella; Wild birds.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400882

Comparison of experimental competitive-exclusion cultures for controlling Salmonella colonization in broiler chicks
Provedor de dados: 58
Autores: Revolledo,Liliana; Ferreira,Claudete Serrano Astolfi; Ferreira,Antonio José Piantino.
The efficacy of three different types of experimental competitive exclusion (CE-A, CE-B and CE-C) cultures against Salmonella Kedougou (SK) NCTC 12173, resistant to nalidixic-acid resistant (NalR), in one-day-old broiler chicks, in four treatments with three replicates in each treatment, was evaluated. The mean logarithmic counts of Salmonella program of feces were 0.4 L in the group treated with CE-A, derived from the whole cecal contents of an adult bird with a mixture of aerobic and anaerobic bacterial cultures; 1.22 in the group treated with CE-B, containing aerobic bacterial culture; 1.00 in the group treated with CE-C, containing anaerobic bacterial culture; and 6.64 in the control group. The percentage of colonized birds varied from 10% to 26.66% in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Competitive exclusion; Salmonella; Broiler chicks.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400014

Occurrence of Salmonella spp. and generic Escherichia coli on beef carcasses sampled at a brazilian slaughterhouse
Provedor de dados: 58
Autores: Silva,Fabiana Fernanda Pacheco da; Horvath,Mariana Bandeira; Silveira,Juliana Guedes; Pieta,Luiza; Tondo,Eduardo Cesar.
A total of 120 beef carcasses were analyzed during processing at a slaughterhouse in southern Brazil. The carcasses were sampled by swab at three different steps of the slaughter line and then they were tested for Salmonella and E. coli. The Salmonella isolates were also examined for antimicrobial susceptibility. Salmonella prevalence distribution was modeled and the probability of contamination was simulated using @Risk program and 10,000 interactions. Results demonstrated that 4 beef carcasses (3.3%) were positive for Salmonella only in the first point. The six isolates of Salmonella were classified: S. Newport (n = 3), S. Saintpaul (n = 2) and S. Anatum (n = 1). No Salmonella strains exhibited resistance to any of the antimicrobials tested. As expected,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; E. coli; Beef carcasses; Slaughter line; Antimicrobial susceptibility.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100004

Antagonistic and protective effects against Salmonella enterica serovar typhimurium by Lactobacillus murinus in the digestive tract of gnotobiotic mice
Provedor de dados: 58
Autores: Vasconcelos,Ana Luiza Sampaio; Nicoli,Jacques Robert; Nardi,Regina Maria Drummond.
In the present study, production of antagonistic substances by Lactobacillus murinus against enteropathogenic bacteria was evaluated in vivo as well as a possible protective effect against an oral challenge with Salmonella Typhimurium using a gnotobiotic animal model. A higher mean survival time (P &lt; 0.05) was observed in L. murinus-associated animals (7.89 ± 3.83 days) when compared with the control ones (4.44 ± 0.73 days). Lactobacillus murinus exerted a potent antagonistic effect in vivo against S. sonnei and to a lesser extent against S. Typhimurium as revealed by inhibitory halos around the feces of Lactobacillus-associated animals. Diameters of inhibitory halos around intestinal contents increased along the intestine tract, following...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antagonism; Salmonella; Lactobacillus; Gnotobiotic mice.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500007

Salmonella spp. em carcaças, carne mecanicamente separada, lingüiças e cortes comerciais de frango
Provedor de dados: 65
Autores: Carvalho,Angela Cleusa de Fátima Banzatto de; Cortez,Ana Lígia Lordello.
Alimentos de origem animal representam papel fundamental na epidemiologia das salmoneloses humanas. Apesar dos avanços tecnológicos, a carne de frango ainda é passível de contaminação bacteriana, especialmente por microrganismos do gênero Salmonella, que podem encontrar-se albergados no trato intestinal ou em outra parte do corpo das aves. O presente trabalho objetivou pesquisar a ocorrência de Salmonella em carne de frango e derivados procedentes da região Nordeste do Estado de São Paulo. Foram analisadas, através do método convencional de cultivo, 45 amostras de carcaças, 60 de carne mecanicamente separada (CMS), 25 de lingüiça de frango, 20 de peito, e 15 de coxa e sobre-coxa. Salmonella spp. foi encontrada em 13,3% (6/45) das carcaças, 25% (15/60) das...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carne mecanicamente separada; Frango; Salmonella.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000600040

Número mais provável de Salmonella isoladas de carcaças de frango resfriadas
Provedor de dados: 65
Autores: Borsoi,Anderlise; Moraes,Hamilton Luis de Souza; Salle,Carlos Tadeu Pippi; Nascimento,Vladimir Pinheiro do.
A Salmonella permanece um importante problema na avicultura e, considerando os patógenos transmitidos por alimentos, aparece como um dos agentes principais em surtos de toxinfecções alimentares. Para auxiliar na avaliação de riscos em adquirir infecção alimentar via carne de frangos que sofreram cocção inadequada, ou através de contaminação cruzada a partir desses animais, torna-se importante determinar a extensão de contaminação por patógenos em carne crua. No presente trabalho, foram analisadas 180 carcaças de frangos resfriadas, adquiridas em varejos, para pesquisa de Salmonella com determinação do número de células da bactéria. Foi utilizado o método do número mais provável (NMP) nos ágares para isolamento verde brilhante com novobiocina (BGN) e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; NMP; Carcaça de frango; Patógeno alimentar.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001100014

Elisa indireto na detecção de Salmonella spp. em lingüiça suína
Provedor de dados: 65
Autores: Loguercio,Andrea Pinto; Aleixo,José Antonio Guimarães; Vargas,Agueda Castagna de; Costa,Mateus Matiuzzi da.
Um teste ELISA, baseado em um anticorpo monoclonal (MAb) específico para uma proteína de membrana externa de Salmonella enterica serovar Enteritidis foi comparado com o método de cultivo tradicional na detecção de Salmonella spp. em 110 amostras de lingüiça suína frescal. A prevalência do patógeno nas amostras foi de 11,82% de acordo com o cultivo tradicional. O teste ELISA revelou sensibilidade, especificidade, valores preditivos positivos e negativos de 100%, 98%, 87% e 100%, respectivamente. Tais achados indicam que, comparado ao sistema tradicional, o teste imunológico foi bastante eficaz na determinação de Salmonella spp. em amostras de lingüiças naturalmente contaminadas.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; ELISA; Lingüiça suína.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000600022

Qualidade sanitária de embutidos coletados no mercado público central de Porto Alegre-RS
Provedor de dados: 65
Autores: Salvatori,Rosângela Uhrig; Bessa,Marjo Cadó; Cardoso,Marisa Ribeiro de Itapema.
Noventa e três amostras de embutidos coletados no Mercado Público Central de Porto Alegre foram analisadas quanto à presença de Salmonella sp. e à contagem de coliformes fecais. Todos as amostras apresentaram ausência de Salmonella sp., enquanto, em relação aos coliformes fecais, constatou-se que quatorze amostras de embutidos frescais encontravam-se acima do padrão estabelecido pela portaria n° 451, em vigor no momento da coleta. Entretanto, se fossem considerados os parâmetros estabelecidos na Resolução n° 12, em vigor atualmente, apenas cinco amostras seriam consideradas fora de padrão. A maioria das amostras com contagens de coliformes fecais acima de 5x10²/g estava armazenadas acima da temperatura adequada no momento da coleta.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Salmonella; Embutidos; Coliformes fecais.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000400031

Fatores associados à soroprevalência de Salmonella em rebanhos comerciais de suínos
Provedor de dados: 65
Autores: Kich,Jalusa Deon; Mores,Nelson; Piffer,Itamar Antonio; Coldebella,Arlei; Amaral,Armando; Ramminger,Lucas; Cardoso,Marisa.
Um estudo transversal foi utilizado para identificar fatores associados à prevalência de suínos sorologicamente positivos para Salmonella nos estados de Santa Catarina e Rio Grande do Sul. Sessenta e cinco granjas foram visitadas uma semana antes do abate dos animais para aplicação de questionário e coletas de ração, água e sangue. A ração foi submetida à pesquisa de Salmonella por isolamento e PCR. Foram testados soros de aproximadamente 40 leitões de cada propriedade utilizando ELISA com antígeno do sorovar Typhimurium. Após a análise de distribuição da prevalência, as granjas foram classificadas em três categorias, baixa (até 40%), média (40-70%) e alta (mais de 70%). Estas categorias constituíram a variável explicada e a pesquisa de Salmonella na...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Suíno; Salmonella; Prevalência; Análise de correspondência; Estudo transversal.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000200024

Antibacterial activity of vegetal extracts against serovars of Salmonella
Provedor de dados: 65
Autores: Voss-Rech,Daiane; Klein,Cátia Silene; Techio,Vânia Helena; Scheuermann,Gerson Neudi; Rech,Gilberto; Fiorentin,Laurimar.
in vitro antibacterial activity of 21 hydroethanolic vegetal extracts was assessed against 20 serovars of Salmonella. Regarding the tested extracts, 85.7% of them presented antibacterial activity. The six active extracts which showed activity on the largest number of serovars and the extract of Eucalyptus sp. were submitted to the determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Of these, six extracts showed bacteriostatic and bactericidal activity with MIC and MBC for Punica granatum (pomegranate) from 20 and 60mg mL-1, for Eugenia jambolana (rose apple) from 40 and 240mg mL-1, Eugenia uniflora (surinam cherry) from 80 and 240mg mL-1, Caryophyllus aromaticus (clove) from 10 and 60mg mL-1, Psidium araca...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibacterial activity; Vegetal extracts; Salmonella.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000200022

Ocorrência de Salmonella spp. em carcaças de frangos industrialmente processadas, procedentes de explorações industriais do Estado de São Paulo, Brasil
Provedor de dados: 65
Autores: Tessari,Eliana Neire Castiglioni; Cardoso,Ana Lucia Sicchiroli Paschoal; Kanashiro,Ana Maria Iba; Stoppa,Greice Filomena Zanatta; Luciano,Renato Luís; Castro,Antonio Guilherme Machado de.
A demanda de alimentos seguros aumentou no mercado. Além disso, a ausência de determinados microrganismos causadores de zoonoses em alimentos é uma exigência de regulamentos nacionais e internacionais. No entanto, mesmo com avanços tecnológicos, a carne de frango ainda é sujeita à contaminação bacteriana, especialmente por microorganismos do gênero Salmonella. Portanto, os objetivos deste trabalho foram: 1) pesquisar a ocorrência de Salmonella em carcaças de frango prontas para serem distribuídas no comércio e procedentes de explorações industriais do Estado de São Paulo e 2) classificar as amostras processadas quanto à presença ou à ausência de Salmonella em 25 gramas do produto. Para a realização do estudo, 116 amostras de carcaças de frango congeladas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Frangos; Carcaças.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000900023

PREVALÊNCIA DE SALMONELLA EM PRODUTOS DE FRANGOS E OVOS DE GALINHA COMERCIALIZADOS EM PELOTAS, RS, BRASIL
Provedor de dados: 65
Autores: Baú,Ana Cristina; Carvalhal,José Beiro; Aleixo,José Antonio Guimarães.
A prevalência de Salmonella em produtos de frangos e ovos de galinhas, os sorovares mais freqüentes e sua sensibilidadea a antimicrobianos, foram investigados em Pelotas, Brasil, no período de maio de 1997 a outubro de 1998. Um total de 124 amostras de produtos de frangos obtidas em supermercados e açougues e 94 amostras de 6 unidades de ovos de galinhas obtidas em supermercados e feiras-livres foram analisadas através do método convencional de cultivo. Entre as amostras de produtos de frangos analisadas, 13 (10,48%) continham salmonelas. Os 13 isolamentos de salmonelas realizados foram sorotipificados como S. enteritidis (10), S. anatum (1) e S. enterica subespécie enterica sorovar 3,10:e,h:- (2). Todas as cepas isoladas foram resistentes à penicilina G e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Aves; Ovos; Resistência a antibióticos.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000200018

Atividade antimicrobiana dos óleos essenciais de orégano, tomilho e canela frente a sorovares de Salmonella enterica de origem avícola
Provedor de dados: 65
Autores: Santurio,Janio Morais; Santurio,Deise Flores; Pozzatti,Patrícia; Moraes,Cristiane; Franchin,Paulo Rogério; Alves,Sydney Hartz.
Avaliou-se a atividade antimicrobiana dos óleos essenciais de orégano (Origanum vulgare), tomilho (Thymus vulgaris) e canela (Cinnamomum zeylanicum) frente a 60 amostras de Salmonella enterica distribuídas entre 20 sorovares, todos isolados de carcaças de aves. A concentração inibitória mínima (CIM) e a concentração bactericida mínima (CBM) foram determinadas para cada isolado, utilizando-se a técnica de microdiluição em caldo. O óleo essencial de orégano evidenciou forte atividade antibacteriana (CIM média = 529mig ml-1 e CBM média= 661mig ml-1), seguido do tomilho (CIM média=961mig ml-1 e CBM média= 1074mig ml-1), com atividade moderada, enquanto que a menor atividade foi observada com o óleo essencial de canela (CIM média= 1335mig ml-1 e CBM média =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Óleos essenciais; Orégano; Tomilho; Canela; Atividade antimicribiana.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000300031

Inducers and autoinducers on Salmonella enterica serovar Typhimurium motility, growth and gene expression
Provedor de dados: 65
Autores: Conceição,Rita de Cássia dos Santos da; Sturbelle,Régis Tuchtenhagen; Timm,Cláudio Dias; Leite,Fábio Pereira Leivas.
Genus Salmonella bacteria are among the major pathogenic microorganisms in food. This bacterium pathogenicity is related to a number of virulence factors, among which its flagella. Flagellum expression is one of the virulence factors modulated by Quorum Sensing. Epinephrine produced by mammals uses the same signaling pathway of the 3 bacteria autoinducer. This study evaluated the effect of molecules inducer (epinephrine) and autoinducers (autoinducer 2 and autoinducer 3) and their association with the motility, growth and expression genes flhC, fliA, fliY, motA, motB e fliC of Salmonella Typhimurium (ST). Initially, ST was inoculated in BHI. Then, motility assays, growth curves and gene expression were performed by testing different concentrations of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quorum sensing; Salmonella; Epinephrine; Autoinducer; Motility.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015001202201

A refrigeração no diagnóstico de Salmonella spp. utilizando o método microbiológico tradicional e reação em cadeia da polimerase em carcaças de frango
Provedor de dados: 65
Autores: Possebon,Fabio Sossai; Costa,Luis Felipe Zuccolo Paschoal da; Yamatogi,Ricardo Seiti; Rodrigues,Marianna Vaz; Sudano,Mateus José; Pinto,José Paes de Almeida Nogueira.
A pesquisa de Salmonella em carcaças de aves tem mostrado resultados discrepantes, dependendo se as amostras foram colhidas ainda na indústria, imediatamente após o chiller ou no comércio varejista, quando se encontram submetidas à refrigeração por vários dias. Técnicas mais sensíveis, tais como a Reação em Cadeia pela Polimerase (PCR), podem fornecer dados importantes sobre o efeito do resfriamento sobre as células do patógeno, comparando seus resultados com os da metodologia microbiológica convencional (MC). Foram colhidas 130 carcaças de frango, sendo que 65 foram obtidas ainda na indústria (pós-chiller) e imediatamente analisadas e 65 carcaças embaladas e estocadas a 5°C por 72 horas (simulando o varejo), sendo então realizada a pesquisa do patógeno...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Salmonella; Frango; PCR; Refrigeração.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000100021

Inspected and non-inspected chilled chicken carcasses commercialized in Viçosa, MG, Brazil: microbiological parameters and Salmonella spp. occurrence
Provedor de dados: 65
Autores: Cossi,Marcus Vinícius Coutinho; Almeida,Michelle Vieira de; Dias,Mariane Rezende; Pinto,Paulo Sérgio de Arruda; Nero,Luís Augusto.
Sixty samples of chilled chicken carcasses submitted (30) and not submitted (30) to Brazilian inspection services were analyzed to investigate if inspected and non-inspected chilled carcasses represented different food safety risks in the region of Viçosa, MG, Brazil. The mean counts of indicator microorganisms (mesophilic aerobes, Enterobacteriaceae, total coliforms and Escherichia coli) of samples belonging to the inspected and non-inspected lots did not present significant differences (P&amp;gt;0.05). Also, no significant differences (P&amp;gt;0.05) were observed for the numbers of Salmonella spp. and E. coli (higher than 2log cfu g-1) between samples submitted or not to inspection. Statistical differences were observed between the two sample classes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inspection; Salmonella; Escherichia coli; Enterobacteriaceae; Hygiene indicators.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000900025

Prevalência de Salmonella sp. em suínos abatidos no Estado de Mato Grosso
Provedor de dados: 65
Autores: Silva,Maria Cristina da; Faria,Givago Silva; Paula,Daphine Ariadne Jesus de; Martins,Rodrigo Prado; Caramori Junior,João Garcia; Kich,Jalusa Deon; Colodel,Edson Moleta; Nakazato,Luciano; Dutra,Valéria.
O presente trabalho teve como objetivo determinar a prevalência de Salmonella sp. em suínos abatidos em frigoríficos sob Inspeção Federal no Estado de Mato Grosso. Amostras de linfonodos mesentéricos e tonsilas de diferentes procedências foram coletadas em três frigoríficos. Das 300 amostras processadas, 50 (16,6%) foram positivas para Salmonella sp., sendo identificados 14 diferentes sorovares. Os sorovares mais freqüentes foram Derby (16%), Typhimurium (14%), London (12%) e Give (12%). Os resultados epidemiológicos obtidos mostram uma baixa prevalência desta bactéria quando comparados com os resultados de estudos realizados em outros Estados.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Suínos; Mato Grosso.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100045

Sorotipos de Salmonella isolados em uma propriedade de suínos de terminação no Sul do Brasil
Provedor de dados: 65
Autores: Michael,Geovana Brenner; Simoneti,Rosalis; Cardoso,Marisa Ribeiro de Itapema; Costa,Marisa da.
Em uma granja de suínos, foram isolados quinze sorotipos diferentes de Salmonella. Dois dos sorotipos isolados, Mbandaka e Tennessee, foram também encontrados na ração fornecida aos animais. Este estudo demonstrou a diversidade de sorotipos que podem estar presentes em uma granja e a necessidade de um melhor entendimento da epidemiologia da infecção por Salmonella.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Sorotipos; Suínos.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000300026

MÉTODOS DE EXTRAÇÃO DE DNA PARA A DETECÇÃO DE Salmonella EM OVOS DE GALINHAS, COM E SEM CASCA, ATRAVÉS DA REAÇÃO EM CADEIA PELA POLIMERASE
Provedor de dados: 65
Autores: Flôres,Maristela Lovato; Nascimento,Vladimir Pinheiro do; Kader,Ivonyr Irene Troglio Abdel; Santos,Luciana Ruschel dos; Pontes,Alexandre Pontes; Salle,Carlos Tadeu Pippi; Lopes,Rui Fernando Felix.
O diagnóstico microbiológico de Salmonella sp em amostras de alimentos é demorado, com cinco diferentes etapas, levando cerca de 120 horas até o resultado final. A utilização da técnica da Reação em Cadeia pela Polimerase (PCR) pode diminuir esse período, porém sofre influência de substâncias presentes na amostra que afetam a reação. O objetivo deste trabalho foi comparar dois métodos de extração de DNA, a extração por tratamento térmico e a pelo fenol-clorofórmio, em amostras de 100 ovos de galinhas domésticas artificialmente contaminados com uma cepa de Salmonella enterica sorovar typhimurium em fase estacionária. O material obtido com as extrações foi submetido à PCR, utilizando-se um par de iniciadores que amplificam um fragmento de 284pb do gene InvA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Ovos; Extração; DNA; PCR.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000200020

Aspectos sanitários do cultivo da alface americana, irrigada com águas recptoras de efluentes urbanos
Provedor de dados: 64
Autores: Biscaro,Guilherme Augusto; Guimarães-Tomazela,Andréa Bogatti; Cruz,Raimundo Leite; Lopes,Marcelo Domingos Chamma.
Avaliou-se a influência da disposição de mangueiras gotejadoras nos canteiros e a injeção ou não de cloro na água de irrigação, nas condições sanitárias do solo e da alface americana irrigada (Lactuca sativa L.) com águas receptoras de efluentes urbanos. Foram realizadas análises microbiológicas de amostras de água do solo e da alface, no decorrer de todo o ciclo de cultivo. Objetivou-se determinar a possível existência de Salmonella spp. e de formas evolutivas de parasitos humanos e a quantidade de coliformes fecais, em pontos e épocas diferentes do experimento, impedindo assim o consumo da alface. Os resultados não indicaram a presença dos dois primeiros em nenhuma das amostras, mas sim de parasitos não humanos (nematóides) de vida livre no solo. Em...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nematóides; Salmonella; Coliformes fecais; Gotejamento.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000100042