Consulta: subjectFacets:"Secondary metabolite"
Registros recuperados: 13
Data/hora: 16/06/2024 19:38:55

Methyl jasmonate induced overproduction of eleutherosides in somatic embryos of Eleutherococcus senticosus cultured in bioreactors
Provedor de dados: 69
Autores: Shohael,Abdullah Mohammad; Murthy,Hosakatte Niranjana; Hahn,Eun-Joo; Paek,Kee-Yoeup.
This study was concentrated on the production of eleutherosides and chlorogenic acid in embryogenic suspension cultures of Eleutherococcus senticosus by exposing them to different concentrations (50-400 µM) of methyl jasmonate (MJ) during the culture period. In the bioreactor cultures, eleutheroside content increased significantly by elicitation of MJ, however, the fresh weight, dry weight and growth ratio of embryos was strongly inhibited by increasing MJ concentrations. The highest total eleutheroside (7.3 fold increment) and chlorogenic acid (3.9 fold increment) yield was obtained with 200 µM MJ treatment. There was 1.4, 3.4 and 14.9 fold increase in the eleutheroside B, E, and E1 production respectively with such elicitation treatment. These results...
Tipo: Journal article
Palavras-chave: Eleutherosides; Elicitation; Methyl jasmonate; Secondary metabolite; Siberian ginseng; Suspension culture.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400016

Toxicity of ethanolic extracts from Lippia origanoides and Gliricidia sepium to Tetranychus cinnabarinus (Boisduval) (Acari: Tetranychidae)
Provedor de dados: 82
Autores: Sivira,A; Sanabria,ME; Valera,N; Vásquez,C.
Botanical compounds with insecticidal and acaricidal activities have been used in pest management with different levels of success. Toxicity of ethanolic extracts obtained from wild oregano (Lippia origanoides) and gliricidia (Gliricidia sepium) to Tetranychus cinnabarinus (Boisduval) were evaluated. Mite population was collected from black bean plants growing in Urachiche Municipality, Yaracuy State, Venezuela. Ethanolic extracts of wild oregano and gliricidia leaves were evaluated at different concentrations (5, 10, 15, and 20%) using the leaf disk immersion technique. The presence of alkaloids, flavonoids, phenols and tannins, essential oils and saponins was verified in the plant material used in our study. Tetranychus cinnabarinus oviposition decreased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Botanical acaricide; Carmine spider mite; Secondary metabolite.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2011000300011

Contribution of gliotoxin to aspergillosis
Provedor de dados: 19
Autores: 豊留, 孝仁; TOYOTOME, Takahito.
Aspergillus species (spp.) are medically important pathogens that cause aspergillosis, and A. fumigatus is the most prevalent causative agent of the disease. Aspergillus spp. produce various secondary metabolites; however, the role of these metabolites in aspergillosis remains unclear. Gliotoxin is a major secondary metabolite produced by A. fumigatus. Several recent studies have reported that gliotoxin contributes to the virulence of A. fumigatus. Here I review studies related to gliotoxin and its contribution to the infection caused by A. fumigatus.
Palavras-chave: Aspergillosis; Aspergillus fumigatus; Gliotoxin; Secondary metabolite.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4157

Effect of the flavonoid rutin on the biology of Spodoptera frugiperda (Lepidoptera: Noctuidae)
Provedor de dados: 1
Autores: Silva, Talita Roberta Ferreira Borges; Almeida, André Cirilo de Sousa; Moura, Tony de Lima; Silva, Anderson Rodrigo da; Freitas, Silvia de Sousa; Jesus, Flávio Gonçalves.
The fall armyworm Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) is a major pest of maize crops in Brazil. The effects of plant metabolites on the biology and behavior of insects is little studied. The aim of the study was to evaluate the activity of rutin on the biology of the S. frugiperda by using artificial diets containing rutin. The study evaluated four treatments: regular diet (control group) and diets containing 1.0, 2.0 and 3.0 mg g-1 of rutin. The following biological variables parameters of the larvae were evaluated daily: development time (days), larval and pupal weight (g) and viability (%), adult longevity and total life cycle (days). A completely randomized experimental design was used with 25 replication. The rutin flavonoid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fall armyworm; Insecticide plant; Secondary metabolite; Flavonoid Fitossanidade.
Ano: 2016
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/27956

Hepatic toxicity caused by PLGA-microspheres containing usnic acid from the lichen C ladonia substellata (AHTI) during pregnancy in Wistar rats
Provedor de dados: 42
Autores: MARINHO,KETSIA S.N.; ANTONIO,ERIVALDO A.; SILVA,CAMILLA V.N.S.; SILVA,KEILA T. DA; TEIXEIRA,VALÉRIA W.; AGUIAR JUNIOR,FRANCISCO C.A. DE; SANTOS,KATHARINE R.P. DOS; SILVA,NICÁCIO H. DA; SANTOS,NOEMIA P.S.
ABSTRACT This study aimed to evaluate the teratogenic and hepatotoxic potential of the usnic acid encapsulated into PLGA-microspheres. In total, 12 female Wistar rats in pregnancy were randomly distributed in the control group (n= 6) that received 1.0 mL of physiological solution and treatment group (n= 6) that received 25 mg/kg of encapsulated usnic acid by oral administration. All females were euthanized at day 20 of pregnancy and their fetuses were removed and analyzed. During the pregnancy was observed a reduction in weight gain. There was no difference in serum transaminases levels analyzed as well as any difference in liver weight in both groups. The histomorphometric analysis of the liver from the treatment group revealed an increase in number of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Encapsulation; Fetotoxicity; Hepatotoxicity; Secondary metabolite.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301073

Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ-pirone by Aspergillus flavus
Provedor de dados: 42
Autores: Ferreira,Nelson R.; Sarquis,Maria Inez M.; Alves,Cláudio N.; Santos,Alberdan S.
The sucrose hydrolysis and the preference of consumption of glucose instead of fructose were investigated for the production of 5-hydroxy-2-hydroxymethyl-γ-pyrone (HHMP) in the presence of Aspergillus flavus IOC 3974 cultivated in liquid Czapeck medium. Standardized 0.5g of pellets were transferred as inoculum into twelve conical flasks of 250 ml containing 100 ml of medium with different sucrose concentration, which was kept at 120 rpm and 28"C for 16 days without pH adjustment. Aliquots of 500μl of the broth culture were withdrawn at 24 h intervals and analyzed. The major yield of HHMP was 26g l-1 in 120g l-1 of sucrose. At these conditions, A. flavus produced an invertase capable of hydrolyzing 65% of total sucrose concentration in 24h, and an isomerase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus flavus; Biotransformation; Kojic acid; Secondary metabolite; Fructose-isomerase.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000300004

Does mechanical damage on soybean induces the production of flavonoids?
Provedor de dados: 42
Autores: SILVA,PAULO LUIZ DA; CORDEIRO,GLÁUCIA; SILVA,CAROLINA R. DA; BARROS,RAFAEL A.; SILVA,CAMILA R. DA; ZANUNCIO,JOSÉ C.; CAMPOS,WELLINGTON G.; OLIVEIRA,MARIA G.A.
Abstract The response of plants to grazing includes the production of chemical defense compounds such as proteases inhibitors and secondary metabolites as flavonoids, which makes them less palatable to feeding and negatively affecting the physiology of insects. The aim of this study was to evaluate the phytochemical response of soybean cultivars (Glycine max (L.) Merrill) resistant (IAC-17, IAC-24) and susceptible (IAC-P1) to insects after mechanical damage. These cultivars were mechanically injured, and after 24 hours samples of these plants were analyzed by HPLC to identify and quantify flavonoids. The flavonoids daidzein, quercetin, and rutin were quantified, with the highest concentration of daidzin in soybean cultivars after mechanical damage. Rutin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Flavonoid; Herbivory; Mechanical damage; Secondary metabolite.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703415

Induction of secondary metabolite production by UV-C radiation in Vitis vinifera L. Öküzgözü callus cultures
Provedor de dados: 48
Autores: Cetin,Emine Sema.
BACKGROUND: The aim of the present work was to examine the role of UV-C irradiation on the production of secondary metabolites (total phenolic, total flavanols, total flavonols, catechin, ferulic acid and trans-resveratrol in phenolic compounds and &amp;#945;-, &amp;#946;-, &amp;#947;- &amp;#948;-tocopherols) in callus cultures. Studies on the effects of UV-C treatment on callus culture are seldom and generally focused on UV-B. However UV-C radiation play an important role in accumule secondary metabolites. RESULTS: In this study, callus cultures from Öküzgözü grape cultivar were initiated from leaf petiole explants. Calli formed after 6 weeks on the medium supplemented with 0.5 mg L-1 benzylaminopurine (BA), 0.5 mg L-1 indole acetic acid (IAA) on B5...
Tipo: Journal article
Palavras-chave: Callus; Secondary metabolite; UV-C treatment.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100037

The effects of cadmium chloride on secondary metabolite production in Vitis vinifera cv. cell suspension cultures
Provedor de dados: 48
Autores: Cetin,Emine Sema; Babalik,Zehra; Hallac-Turk,Filiz; Gokturk-Baydar,Nilgun.
BACKGROUND: Plant secondary metabolites are possess several biological activities such as anti-mutagenic, anti-carcinogenic, anti-aging, etc. Cell suspension culture is one of the most effective systems to produce secondary metabolites. It is possible to increase the phenolic compounds and tocopherols by using cell suspensions. Studies on tocopherols production by cell suspension cultures are seldom and generally focused on seed oil plants. Although fresh grape, grape seed, pomace and grape seed oil had tocopherols, with our best knowledge, there is no research on tocopherol accumulation in the grape cell suspension cultures. In this study, it was aimed to determine the effects of cadmium chloride treatments on secondary metabolite production in cell...
Tipo: Journal article
Palavras-chave: Cadmium chloride; Cell; Secondary metabolite.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100047

Phytochemical and Morphological Evidences for Shikonin Production by Plant Cell Cultures of Onosma sericeum Willd
Provedor de dados: 52
Autores: Gharehmatrossian,Sormeh; Popov,Yu; Ghorbanli,Mahlagha; Safaeian,Shila; Iranbakhsh,Alireza.
ABSTRACT Shoot regeneration, callus growth, and biosynthesis of shikonin in callus cultures of Onosma sericeum were examined. Plant tissue culture was used as an alternative method for increasing the production of shikonin, a secondary metabolite. The isolated cultures were subjected to abiotic factors such as light, plant growth regulators, and nutritional factors. Identification was carried out by High- Performance Liquid Chromatography (HPLC) after 10th subculture. Nodal explants were incubated in Murashige and Skoog (MS) medium along with different combination of growth hormones. Shoot regeneration from calli were achieved on MS basal medium supplemented with 3 mg/l 6-benzylaminopurine (BAP) and 0.5 mg/l Naphthalene acetic acid (NAA) under light cycle....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth index; Medicinal plant; Plant regeneration; Secondary metabolite.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100428

LC50 of the Peptide Produced by the Entomopathogenic Fungus Nomuraea rileyi (Farlow) Samson Active Against Third Instar Larvae of Anticarsia gemmatalis (Lep.: Noctuidae)
Provedor de dados: 52
Autores: Onofre,Sideney Becker; Riveros Gonzalez,Raul; Messias,Cláudio Luiz; Azevedo,João Lúcio; Barros,Neiva Monteiro de.
The entomopathogenic fungus Nomuraea rileyi (Farlow) Samson produced a peptide active against Anticarsia gemmatalis 3rd instar larvae. To produce this peptide, N. rileyi was cultivated aerobically in Saboraud, maltose, yeast-extract broth at 26 ± 1ºC for 12 days, after which the medium was filtered and separated in a liquid/liquid extractor, concentrated and the peptide purified chromatographically. The crystals obtained were kept refrigerated until needed for LC50 analysis. The LC50 of this peptide against A. gemmatalis 3rd instar larvae was determined in triplicate experiments using solutions containing 1.0, 0.2, 0.1, 0.01, 0.001 and 0.0001 mg/ml of N. rileyi peptide. The results of these experiments were used to calculate a linear equation in which Y =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anticarsia gemmatalis; Nomuraea rileyi; Integrated control; Secondary metabolite; Toxins.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300004

Optimization of zofimarin production by an endophytic fungus, Xylaria sp. Acra L38
Provedor de dados: 58
Autores: Chaichanan,Jirapan; Wiyakrutta,Suthep; Pongtharangkul,Thunyarat; Isarangkul,Duangnate; Meevootisom,Vithaya.
To optimize the medium for high zofimarin production, sucrose maltose, glucose, tryptone and peptone were used in an orthogonal array design experiment, where the highest value of zofimarin produced was 25.6 µg/mL. This value was about 3 times higher than that obtained with Czapek yeast extract (CzYE) culture medium. A study with Plackett-Burman design showed that sucrose, maltose, glucose and NaNO3 were significant factors in zofimarin production. Further studies using central composite design (CCD) showed the significance of glucose and the interactions of these critical components affecting zofimarin production. Multiple regression analysis of the data yielded a poor fit as shown by the mismatch of the model with these variable factors. When a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zofimarin; Secondary metabolite; Medium optimization; Statistical methods; Endophytic fungus.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100042

Chemical characterization of bioactive compounds from the endophytic fungus Diaporthe helianthi isolated from Luehea divaricata
Provedor de dados: 58
Autores: Specian,Vânia; Sarragiotto,Maria Helena; Pamphile,João Alencar; Clemente,Edmar.
Endophytic microorganisms, defined as fungi or bacteria that colonize the interior of plants without causing any immediate negative effects or damages, have reciprocal relationships with host plants. In some cases their presence is beneficial to the host due to the synthesis of bioactive compounds, among which several alcohols, esters, ketones and others that may react with other compounds and may be lethal to pathogenic microorganisms. Diaporthe helianthi (Phomopsis helianthi in its anamorphic phase) is available worldwide, especially in Europe, Asia and America. Isolated in Europe as an agent of the sunflower stem cancer, it has also been endophytically isolated from tropical and temperate plants. A D. helianthi strain isolated from Luehea divaricata has...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endophytic fungi; Diaporthe helianthi; Secondary metabolite; Phenolic compound; Tyrosol.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300045