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Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Morais,Glaucia Almeida de; Takaki,Massanori.
Determination of α- and β-amylase activity in the extracts of cotyledons of Phaseolus vulgaris L. cv. cariocawas done using selective inactivation of α-amylase by lowering the pH of the incubation medium or by the use of EDTA as inhibitor or selective inactivation of β-amylase by the use of HgCl2 or by heating to 70ºC in the presence of CaCl2; and still by using the reagent starch azure for specific determination of α-amylase. Results indicated that the methods used were inappropriate in this case, being indicated the determination of total amylase activity.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Α-amylase; Β-amylase; Determination; Selective inactivation; Phaseolus vulgaris.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100003