Consulta: subjectFacets:"Serine-thiol proteinase"
Registros recuperados: 1
Data/hora: 16/06/2024 06:18:09

Detection of the basement membrane-degrading proteolytic activity of Paracoccidioides brasiliensis after SDS-PAGE using agarose overlays containing Abz-MKALTLQ-EDDnp
Provedor de dados: 56
Autores: Puccia,R.; Juliano,M.A.; Juliano,L.; Travassos,L.R.; Carmona,A.K.
We have characterized, in the Paracoccidioides brasiliensis yeast phase, an exocellular SH-dependent serine proteinase activity against Abz-MKRLTL-EDDnp and analogous fluorescent-quenched peptides, and showed that it is also active against constituents of the basement membrane in vitro. In the present study, we separated the components of P. brasiliensis culture filtrates by electrophoresis and demonstrated that the serine-thiol exocellular proteinase has a diffuse and heterogeneous migration by SDS-PAGE, localizing in a region between 69 and 43 kDa. The hydrolytic activity was demonstrable after SDS-PAGE using buffered agarose overlays of Abz-MKALTLQ-EDDnp, following incubation at 37oC, and detection of fluorescent bands with a UV transilluminator....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P. brasiliensis; Serine-thiol proteinase; SDS-PAGE; Fluorescent-quenched peptides.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500019