Consulta: subjectFacets:"Serology"
Registros recuperados: 57
Data/hora: 11/06/2024 10:02:48

Detección, dispersión y muestreo en la erradicación del Citrus Tristeza Virus en plantaciones comerciales de cítricos
Provedor de dados: 32
Autores: Ruiz García, Noé.
La dispersión espacio-temporal del Citrus tristeza virus se caracterizó en Tamaulipas de  diciembre de 2001 a marzo de 2004, en 11 huertos comerciales de naranja dulce injerta-  dos en naranjo agrio. Adicionalmente, se comparó el desempeño de la técnica serológica  inmunoimpresión-ELISA con respecto a DAS-ELISA propuesta en la normatividad ofi-  cial mexicana de la Campaña Nacional contra CTV. El objetivo fue proporcionar bases  epidemiológicas e incorporar criterios de diagnóstico viral en una propuesta de muestreo  con fines de erradicación. Inmunoimpresión-ELISA fue tan confiable (97.8 %) como  DAS-ELISA en identificar árboles positivos, siendo 54.9% más económica e implicó solo  una sexta parte del tiempo de procesado. La detectabilidad en impresiones...
Tipo: Tesis
Palavras-chave: Citrus tristeza virus; Serología; Muestreo; Vectores; Epidemiología; Doctorado; Fitopatología; Fitopatología; Serology; Sampling; Vectors; Epidemiology.
Ano: 2008
URL: http://hdl.handle.net/10521/1348

Detección, disperción y muestreo en la erradicación del Citrus Tristeza Virus en plantaciones comerciales de cítricos
Provedor de dados: 32
Autores: Ruiz García, Noé.
La dispersión espacio-temporal del Citrus tristeza virus se caracterizó en Tamaulipas de  diciembre de 2001 a marzo de 2004, en 11 huertos comerciales de naranja dulce injerta-  dos en naranjo agrio. Adicionalmente, se comparó el desempeño de la técnica serológica  inmunoimpresión-ELISA con respecto a DAS-ELISA propuesta en la normatividad ofi-  cial mexicana de la Campaña Nacional contra CTV. El objetivo fue proporcionar bases  epidemiológicas e incorporar criterios de diagnóstico viral en una propuesta de muestreo  con fines de erradicación. Inmunoimpresión-ELISA fue tan confiable (97.8 %) como  DAS-ELISA en identificar árboles positivos, siendo 54.9% más económica e implicó solo  una sexta parte del tiempo de procesado. La detectabilidad en impresiones...
Tipo: Tesis
Palavras-chave: Citrus tristeza virus; Serología; Muestreo; Vectores; Epidemiología Citrus tristeza virus; Serology; Sampling; Vectors; Epidemiology.
Ano: 2012
URL: http://hdl.handle.net/10521/1020

Distribución espacial y factores de riesgo asociados a la leptospirosis bovina en Veracruz, México.
Provedor de dados: 32
Autores: Cruz Romero, Anabel.
Los objetivos de esta investigación fueron determinar la distribución espacial e identificar los factores de riesgo asociados a la leptospirosis bovina en el estado de Veracruz, México. Se obtuvieron muestras de sangre de 3,454 bovinos de 54 municipios de las regiones Norte, Centro y Sur del estado, de febrero 2008 a diciembre de 2011. Los sueros se analizaron por medio de la técnica de aglutinación microscópica utilizando 12 serovariedades de Leptospira interrogans, se estimaron las seroprevalencias y los factores de riesgo. La seroprevalencia general fue del 3.9%; sin embargo, entre municipios varió de 0 y 44%. La mayoría de las UP con casos positivos se encontraron en la zona centro de Veracruz, y en los  municipios de Cuitláhuac, Papantla, Tlacotalpan...
Palavras-chave: Estudio transversal; Bovinos; Serología; Epidemiología; Cross-sectional study; Bovines; Serology; Epidemiology; Agroecosistemas Tropicales; Doctorado.
Ano: 2013
URL: http://hdl.handle.net/10521/2336

Soroprevalência de cinco enfermidades na produção de caprinos e ovinos de corte no município de Tauá, região dos Inhamuns - Ceará.
Provedor de dados: 14
Autores: FACCIOLI-MARTINS, P. Y.; PINHEIRO, R. R.; ALVES, F. S. F.; SANTOS, V. W. S. dos; ANDRIOLI, A.; CAVALCANTE, A. C. R.; LIMA, A. M. C.; OLIVEIRA, E. L. de; ABREU, A. R. A.
Apresenta o perfil zoossanitário da caprinocultura e ovinocultura de corte na região dos Inhamuns, com ênfase no município de Tauá, por meio do diagnóstico sorológico de cinco doenças infecciosas: Artrite-Encefalite Caprina (CAE), Brucelose Ovina, Língua Azul, Linfadenite Caseosa e Maedi-Visna, para respaldar ações futuras em sanidade animal junto aos produtores.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Brucelose ovina; Sorologia; Prevalência; Brasil; Região Nordeste; Sertão; Semiárido; Caprino; Ovino; Doença animal; Sanidade animal; Linfadenite caseosa; Língua Azul; Diagnostico; Goats; Sheep; Animal diseases; Caseous lymphadenitis; Disease diagnosis; Disease prevalence; Serology; Brazil; Semiarid zones.
Ano: 2017
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1086785

Le concept de biosécurité en pisciculture marine : prévention de l'encéphalite virale chez l'ombrine subtropicale (Sciaenops ocellatus) en Martinique
Provedor de dados: 5
Autores: Adee, Fabrice.
In 1985, red drum (Sciaenops ocellatus) was introduced in Martinique in arder to replace sea bass fish farming. Nodavirus antibodies were detected in the broodstock at the IFREMER station in Robert. Nodavirus is responsible for viral nervous necrosis, a disease that is spreading quickly in worlwide marine aquaculture. A biosecurity process was initiated at the Robert hatchery to select seronegative breeders and produce nodavirus free larvae. This process, based on a serological follow-up of fish antibodies by ELISA, allowed a reduction of the number of seropositive fish. A complementary serological study revealed a low incidence of nodavirus contamination in nodavirus free larvae placed in sea cages on different production sites
Tipo: Text
Palavras-chave: Nodavirus; Encéphalite virale; Sérologie; Ombrine subtropicale; Epidemiologie; Aquaculture; Nodavirus; Viral nervous necrosis; Serology; Red drum; Epidemiology; Aquaculture.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/00446/55734/57386.pdf

Occurrence and risk factors associated with canine leptospirosis
Provedor de dados: 80
Autores: Kikuti,M; Langoni,H; Nobrega,DN; Corrêa,APFL; Ullmann,LS.
Leptospirosis is a globally distributed emerging zoonosis. Dogs are commonly affected and although other serovars can cause canine leptospirosis, Leptospira interrogans serovar Canicola is primary found in these animals. A retrospective study was conducted using a database of 1195 dogs tested for Leptospira infection from 2003 to 2010 at the Laboratory of Zoonosis Diagnosis at the Veterinary Hospital of São Paulo State University (UNESP) in Botucatu, São Paulo state, Brazil. The seroprevalence of infected dogs was 20.08% (240/1195), and the most prevalent serovars were Canicola (6.7%), Copenhageni (5.0%), Icterohaemorrhagiae (2.9%), Autumnalis (2.9%), Pyrogenes (2.8%), Pomona (2.0%), Hardjo (2.0%), Australis (1.8%), Bratislava (1.6%), Cynopteri (1.4%),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira spp.; Dog; Serology; Epidemiology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100016

Leptospira spp. infection in sheep herds in southeast Brazil
Provedor de dados: 80
Autores: Barbante,Priscila; Shimabukuro,Fabio H; Langoni,Helio; Richini-Pereira,Virgínia B; Lucheis,Simone B.
Background With the aim of studying Leptospira spp. infection in sheep herds, blood samples and respective kidney and liver fragments were collected from 100 animals from twenty different properties during slaughter at a meat company in the Sorocaba region, São Paulo state, southeast Brazil. The microscopic agglutination test (MAT) was performed with 29 strains of Leptospira spp. To identify the agent in the liver and kidney, 100 samples of each tissue were submitted to culture in Fletcher medium and analyzed by the polymerase chain reaction (PCR) for Leptospira spp.Results MAT detected 23 samples serologically positive for one or more Leptospira spp. serovars and significantly more for Autumnalis. Eight (4%) samples were positive in culture (four kidneys...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Ovine; Serology; Culture; PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200325

Serological profile of anti-Toxoplasma gondii antibodies in apparently healthy dogs of the city of Botucatu, São Paulo State, Brazil
Provedor de dados: 80
Autores: Langoni,H.; Modolo,J. R.; Pezerico,S. B.; Silva,R. C.; Castro,A. P. B.; Silva,A. V. da; Padovani,C. R.
Toxoplasmosis is a zoonosis caused by Toxoplasma gondii, a coccidian protozoan of worldwide distribution. The seroprevalence in canine population can be an alternative for measuring T. gondii urban spreading. A total of 780 blood samples from dogs were collected, during the yearly anti-rabies campaign, carried out by the Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Husbandry (FMVZ), São Paulo State University, UNESP, together with the county health authorities, in August 1999. Using Indirect Fluorescent Antibody Test (IFAT) for detecting antibodies anti-T. gondii in the sera samples, we observed that 258 dogs (33.1%) were positive. The associations between the serological results and the epidemiological...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Toxoplasma gondii; Dog; Serology.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100012

Anti-leptospirose agglutinins in equine sera, from São Paulo, Goias, and Mato Grosso do Sul, Brazil, 1996-2001
Provedor de dados: 80
Autores: Langoni,H.; Da Silva,A.V.; Pezerico,S.B.; De Lima,V.Y.
Equine leptospirosis can present a non-symptomatic form, an acute clinical form, or even develop chronically, causing reproductive alterations, such as abortion and recurrent uveitis. Since the prevalence of leptospirosis in several countries and regions is widely reported, the objective of this study was to verify the prevailing equine leptospirosis in different regions of Brazil. Sera from 1402 blood samples from horses of different age, sex, breed, and purpose were examined. These samples came from southeastern and central west states of Brazil. The method utilized was the Microscopic Agglutination Test (MAT), with 12 different Leptospira serovars. From the sera tested, 754 (54%) were positive for one (385) or more (372) serovars. These results were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Horse; Serology.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300003

American visceral Leishmaniasis: a case report
Provedor de dados: 80
Autores: Langoni,H.; Lucheis,S. B.; Da Silva,R. C.; Castro,A. P. B.; Paes,A. C.
Visceral leishmaniasis is a zoonotic disease caused by parasites of the Leishmania genus. Dog is the major source of infection to man, especially in urban areas. The authors report a case of visceral leishmaniasis in a pit bull female dog from Bocaina, São Paulo, Brazil. The animal presented clinical signs compatible with leishmaniasis, including skin lesions in the body and partial damage of the external ears. The indirect fluorescent antibody test (IFAT) demonstrated a titer of 1280, and promastigote forms of Leishmania sp were isolated by the culture of bone marrow puncture. Cytological analysis of the lymph node and smear of the bone marrow puncture revealed macrophages containing amastigote forms of Leishmania sp in their inner region. The test of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania; Zoonosis; Dog; Diagnosis; Hemoculture; Serology; Polymerase chain reaction (PCR).
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300012

Comparative serology of the marine fish pathogen &lt;i&gt;Vibrio anguillarum&lt;/i&gt;
Provedor de dados: 27
Autores: Grisez, L.; Ollevier, F.P.
The different serotyping systems, based on thermostable O antigens, reported for &lt;i&gt;Vibrio anguillarum&lt;/i&gt; and &lt;i&gt;V. ordalii&lt;/i&gt; were compared by quantitative agglutination, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and subsequent silver staining or Western blotting (immunoblotting) of purified lipopolysaccharide (LPS), using polyclonal rabbit antisera. The results demonstrate that 16 different serotypes within &lt;i&gt;V. anguillarum&lt;/i&gt; (designated 01 to 016) can be distinguished. Each of these serotypes is characterized by a distinct polysaccharide banding pattern, as revealed by silver-stained gels of purified LPS. The comparative analysis allowed a complete alignment of the different stereotypes for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pathogens; Serology; Vibrio anguillarum.
Ano: 1995
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2970

Simple and multiple resistances to viruses in cowpea genotypes
Provedor de dados: 86
Autores: Lima,José Albersio Araujo; Silva,Ana Kelly Firmino da; Aragão,Maria do Livramento; Ferreira,Nádia Rutielly de Araújo; Teófilo,Elizita Maria.
The objective of this work was to identify new sources of simple and multiple resistances to Cowpea severe mosaic virus (CPSMV), Cowpea aphid-borne mosaic virus (CABMV) and Cucumber mosaic virus (CMV) isolates in cowpea (Vigna unguiculata). Thirty-three genotypes from the germplasm bank of Universidade Federal do Ceará were tested as to their resistance to four CPSMV isolates, two CABMV isolates and one CMV isolate. Twenty-five days after the first virus inoculations, all inoculated plants, including the asymptomatic ones, were tested by serology. Genotypes were classified as: immune, plants without symptoms and negative serology; resistant, plants with mild mosaic and positive serology; susceptible, plants with mosaic and positive serology; and highly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cowpea aphid-borne mosaic virus; Cowpea severe mosaic virus; Cucumber mosaic virus; Vigna unguiculata; Serology; Virus control.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011001100003

Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
Provedor de dados: 89
Autores: Oliveira,GH; Berchieri Jr,A; Montassier,HJ.
This study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken; ELISA; Enzyme; Salmonella sp; Serology.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000100008

Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
Provedor de dados: 89
Autores: Oliveira,GH de&lt;A NAME="nt01a"&gt;&lt;/A&gt;; Berchieri Júnior,Â; Montassier,HJ; Fernandes,AC.
This study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidase and alkaline phosphatase conjugates. According to the results, the antigen, sera and conjugate concentrations were optimized. In addition, the assay using alkaline phosphatase and peroxidase conjugates was helpful to distinguish positive serological reaction to serotypes Gallinarum and Pullorum from Enteritidis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chickens; Conjugate; ELISA; Salmonella sp; Serology.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200007

Serosurvey of selected avian pathogens in brazilian commercial Rheas (Rhea americana) and Ostriches (Struthio camelus)
Provedor de dados: 89
Autores: Freitas Neto,OC de; Carrasco,AOT; Raso,TF; Sousa,RLM; Berchieri Júnior,A; Pinto,AA.
Ratite farming of has expanded worldwide. Due to the intensive farming methods used by ratite producers, preventive medicine practices should be established. In this context, the surveillance and control of some avian pathogens are essential for the success of the ratite industry; however, little is known on the health status of ratites in Brazil. Therefore, the prevalence of antibodies against Newcastle Disease virus, Chlamydophila psittaci, Mycoplasma gallisepticum, Mycoplasma synoviae, and Salmonella Pullorum were evaluated in 100 serum samples collected from commercial ostriches and in 80 serum samples from commercial rheas reared in Brazil. All sampled animals were clinically healthy. The results showed that all ostriches and rheas were serologically...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlamydophila psittaci; Mycoplasma spp.; Newcastle Disease virus; Ostrich; Rhea; Salmonella spp.; Serology.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000400010

Serological Diagnosis of Influenza A Subtype H1 on Family Poultry of Belo Horizonte, Minas Gerais and Santa Maria, Rio Grande Do Sul, in Brazil.
Provedor de dados: 89
Autores: Castro Filho,RPL; Torres,ACD; Marin,SY; Diniz,SA; Matos,JCC; Mureb,EN; Resende,M; Martins,NRS.
ABSTRACT Serum samples (n=687) from Gallus gallus domesticus were collected for the investigation of antibodies to avian influenza virus (AIV-A) in the family poultry of the surrounding counties of Santa Maria/RS and the metropolitan region of Belo Horizonte/MG, totaling twenty different counties. Additional samples of seventeen (n=17) free-flying ducks (C. moschata pure or hybrid with Anas platyrhynchos) were collected in Belo Horizonte. The chosen tests for the survey were performed as described by the World Organization for Animal Health (OIE), including agar gel immunodiffusion (AGID) for antibodies to AIV-A nucleoprotein (N) and haemagglutination- inhibition (HI) for antibodies to subtype H1. Out of the 704 serum tests performed by AGID, eight (8/704)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anas platyrhynchos; Avian influenza; Brazil; Cairina moschata; Family poultry; Gallus gallus domesticus; Serology.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400811

Infectious bursal disease virus: case report and experimental studies in vaccinated and unvaccinated SPF chickens and commercial broiler chicks
Provedor de dados: 89
Autores: Scanavini Neto,H; Ito,NMK; Miyaji,CI; Lima,E de A; Okabayashi,S; Corrêa,ARA; Eleutério,GC; Zuanaze,MA.
IBDV Gm 11 (Simbios eleven-molecular group) has been detected since 1997 in many farms of commercial broilers and layers causing high mortality (2 to 15%) and severe macro and microscopic damage in cloacal bursae, spleen, thymus, kidney and liver. Five serial passages of 2050/97-Gm 11 IBDV sample by CAM route in SPF chicken's embryonated eggs did not elicit increased embryo mortality. High mortality (100%) of 21 day-old SPF leghorn chickens and severe bursal and splenic lesions were seen from 24 up to 48 hours after eye-drop inoculation of 2050/97 strain (50 mL of 10-2 dilution of 10% bursae homogenate). Mortality was not detected when vaccinated SPF and broiler chickens were inoculated. One dead bird was found among ten challenged unvaccinated broilers....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Challenge; Histopathology; IBD; Pathogenicity; Serology.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000100006

Serologic response of SPF chickens to live vaccines and other strains of Mycoplasma gallisepticum
Provedor de dados: 89
Autores: Nascimento,ER do; Polo,P de A; Pereira,VL de A; Barreto,ML; Nascimento,M da GF do; Zuanaze,MAF; Corrêa,ARA; Silva,R de CF.
False positive serologic reactions and difficulties in the diagnosis of Mycoplasma gallisepticum (MG) in chickens have increased lately as a result of infection by low virulent MG strains and the use of live MG vaccines in poultry. The objective of this study was to evaluate the serologic responses of SPF chickens exposed to the three commercially available live MG vaccines, and one low virulent MG strain (MG-70), contributing to the diagnosis and monitoring of MG infection in birds. Six groups of SPF chickens were used. The control group was not infected nor challenged; one group was infected with the low virulent strain MG-70 (MG-70); three groups were immunized and named after the MG vaccine used, i.e., MG-6/85, MG-ts11, and MG-F; and finally one group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma; Poultry; Serology; Vaccination.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000100007

Test profiles of broiler breeder flocks housed in farms with endemic Mycoplasma synoviae infection
Provedor de dados: 89
Autores: Fiorentin,L; Mores,MAZ; Trevisol,IM; Antunes,SC; Costa,JLA; Soncini,RA; Vieira,ND.
There is a need for a better understanding of the epidemiology of Mycoplasma synoviae (MS) infection in broiler breeders in Brazil. Many features of the infection remain unrecognizable, because there are no clinical signs of the disease. A detailed testing was performed at each 6 to 8 weeks in three MS-free flocks introduced in farms with endemic MS infection for a follow-up epidemiological study. Every flock was monitored by polymerase chain reaction (PCR), by serum plate agglutination (SPA) and hemagglutination inhibition (HI) for serology studies, and isolation of mycoplasmas from tracheal swabs. PCR was found to be the most sensitive test, detecting early MS infection. Serology was positive in less than 50% of the sera and MS was isolated only between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler breeders; Mycoplasma synoviae; PCR; Serology; Survey; Transmission.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2003000100005

Elisa evaluation of the levels of antibodies against Infectious Bronchitis Virus in laying hens using egg yolk as substrate
Provedor de dados: 89
Autores: Rauber,RH; Flôres,ML; Pereira,CE; Grigulo,M.
The present work was carried out to compare Infectious Bronchitis Virus (IBV) antibody titers in serum and egg yolk samples from laying hens. Sixty paired blood and egg samples were collected from laying hens of two farms. Serum samples were frozen, while egg yolk samples were diluted (1:500) before freezing. Serum and yolk samples were tested for the presence of IBV antibodies by indirect ELISA (commercial kit) and titers were compared by a correlation test (alpha=0.05). There was a high correlation (r=0.62) between the two kinds of samples, which means that titers of IBV antibodies in the egg yolk and in serum samples are quite the same. Considering that blood collection causes deep stress that leads to economic losses, and since eggs are collected daily...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibody; Egg yolk; ELISA; Infectious bronchitis; Serology.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200008

DIAGNOSTIC DEVELOPMENT FOR WILD FIRE SURVEILLANCE
Provedor de dados: 100
Autores: Álvarez,Elba; Iglesia,Aleika.
Information about the characterization and diagnostic of wild fire was compiled. This bacterial disease, exotic for Cuba, is caused by Pseudomonas syringae pv. tabaci. The species Pseudomonas syringae is traditionally divided into pathovars depending on the host range and symptoms. For its study, different phenotypic and genotypic methods are used. Such methods allow the traditional taxonomic confirmation of this pathovar within the species. Serological and molecular techniques such as: ELISA tests, immunofluorescence, polymerase chain reaction (PCR) are also used for the characterization and detection of the bacterium. Such techniques are very useful and sensible for the diagnostic.
Tipo: Journal article
Palavras-chave: Pseudomonas syringae pv. tabaci; Rep-PCR; Pathogenicity; Serology.
Ano: 2007
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522007000300001

HUMORAL RESPONSE TO DIFFERENT VACCINATION SCHEMES AGAINST CLASSICAL SWINE FEVER (CSF) SUCCESSIVELY APPLIED DURING AN OUTBREAK OF THE DISEASE
Provedor de dados: 101
Autores: Percedo,María Irian; Alfonso,P; Frías,María Teresa; Díaz de Arce,Heydi; Barrera,Maritza; Fonseca,O; Castell,Sara.
Vaccination against classical swine fever (CSF) is an essential measure for its control in endemic areas, and the Chinese Strain has been used with success in already disease free countries. Nevertheless the complexity of the factors interacting on the epidemiology and control of CSF, when the expected success is not reached with the vaccination of premises, doubts always fall on the vaccine used and the vaccination scheme applied In consecuences, many changes in vaccination scheme by the breeders are frecuently observed whitout positive influence in CSF control. To clarify the CSF recurrence in a herd vaccinated by a different scheme as the one recommended in the national control program, serological samples were taken to different animal groups in three...
Tipo: Journal article
Palavras-chave: Classical swine fever; Antibodies; Vaccination; Swine; Serology; Focus.
Ano: 2009
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2009000300004

Serological survey of Mycobacterium bovis, Brucella abortus and Borrelia burgdorferi in water buffaloes in the northern region of Brazil
Provedor de dados: 101
Autores: Barbosa da Silva,Jenevaldo; da Fonseca,Adivaldo Henrique; Barbosa,José Diomedes.
The largest buffalo herds are in the northern region of Brazil, so few studies have been conducted to assess the prevalence of selected parasitic diseases in buffalo herds. The present study was therefore conducted to research the epidemiology of Borrelia burgdorferi, Mycobacterium bovis and Brucella abortus in water buffaloes in the north region of Brazil. A total of 4796 buffalo blood samples were randomly collected from five provinces and simultaneously analyzed by ELISA, tuberculin test, CFT and 2ME. The occurrence of B. burgdorferi in buffalo was 75% by ELISA. The tuberculin testing prevalence of M. bovis was 4.6%. The overall prevalence of B. abortus was 4.8% and 4.6% by CFT and 2ME, respectively. Thus, it is concluded that all agents studied were...
Tipo: Journal article
Palavras-chave: Borreliosis; Brucellosis; Prevalence; Serology; Tuberculosis.
Ano: 2014
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2014000100006

Progress report on corn downy mildew research at Kasetsart University, 1976
Provedor de dados: 106
Autores: Udom Pupipat; Thammasak Sommartya; Chiradej Chamswarng; Phatak, H.C.; Vichit Kriengyakul; Kitti Choonhawongse; Uraporn Sardsud.
1 ill.
Palavras-chave: Corn; Maize; Downy mildew; Serology; Pathogenicity; Infectivity; ข้าวโพด; โรคราน้ำค้าง.
Ano: 1976
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/3871

Expression of Grapevine leafroll-associated virus 3 coat protein gene in Escherichia coli and production of polyclonal antibodies
Provedor de dados: 102
Autores: Fajardo,Thor V.M.; Barros,Danielle R.; Nickel,Osmar; Kuhn,Gilmar B.; Zerbini,F. Murilo.
Grapevine leafroll-associated virus 3 (GLRaV-3), the main viral species of the grapevine leafroll complex, causes yield and quality reduction in grapes (Vitis spp.). The coat protein gene was RT-PCR-amplified from total RNA extracted from infected grapevine leaves and the amplified fragment was cloned and completely sequenced. The fragment was subsequently subcloned into the pRSET-C expression vector. The recombinant plasmid was used to transform Escherichia coli BL21:DE3 and express the capsid protein. The coat protein, fused to a 6 His-tag, was purified by affinity chromatography using an Ni-NTA resin. The identity of the purified protein was confirmed by SDS-PAGE and Western blot. The in vitro-expressed protein was quantified and used for rabbit...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Grapevine; GLRaV-3; Recombinant protein; Serology; Antibodies.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000600007

Tomato chlorotic spot virus in hydroponically-grown lettuce in São Paulo State, Brazil
Provedor de dados: 102
Autores: Colariccio,Addolorata; Eiras,Marcelo; Chaves,Alexandre L. R.; Harakava,Ricardo; Chagas,César M.
In the regions of Campinas and Sumaré, São Paulo, Brazil, hidroponically grown crops of Lettuce (Lactuca sativa) cv. Verônica, which showed virus-like symptoms were examined by electron microscope, biological, serological and molecular tests. Pleomorphic, enveloped particles (80-100 nm in diameter) were always detected in these samples. Experimentally inoculated host plants, including lettuce, reacted with tospoviruses-induced symptoms. Some differences were observed in Gomphrena globosa, which reacted by showing local lesions and systemic mosaic. Two isolates of Tomato chlorotic spot virus (TCSV) were identified by DAS-ELISA and by RT-PCR. The sequencing and alignment of the RT-PCR coat protein amplified fragments have indicated a high degree of homology...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tospovirus; Serology; RT-PCR; Sequencing.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000300012

Production of policlonal antisera specific to plant viruses by rabbit oral immunization
Provedor de dados: 102
Autores: LIMA,J. ALBÉRSIO A.; LIMA,ROBERTO C.A.; GONÇALVES,M. FÁTIMA B.
Serological techniques are of great importance for plant virus identification and characterization. The major limiting factor for using these techniques for plant virus identification is the requirement of a good virus purified preparation to be used in immunizing animals for antiserum production. In the present study, two New Zealand rabbits were orally immunized with extracts from cowpea (Vigna unguiculata) plants systemically infected with Cowpea severe mosaic virus (CPSMV) and with extracts from papaya (Carica papaya) infected with Papaya lethal yellowing virus (PLYV). The leaf extracts were prepared in saline solution 0.15 M in the rate of 1:1 (w/v) and clarified by a centrifugation of 10,000 g for 10 min. The clarified extracts containing the viruses...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Serology; Immunology; Virus identification; CPSMV; PLYV.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000400016

Seroprevalence for brucellosis and leptospirosis in dogs from Belém and Castanhal, State of Pará, Brazil
Provedor de dados: 36
Autores: PAZ,Giselle Souza da; ROCHA,Katarine de Souza; LIMA,Michele de Souza; JORGE,Ediene Moura; PANTOJA,José Carlos Figueiredo; MORAES,Carla Cristina Guimarães de; LANGONI,Helio.
Brucellosis and leptospirosis are widely spread bacterial infections and dogs are the most important source of infection and reservoir for diseases. Dogs can disseminate the agents in the environment and transmit them to humans and/or other animals. The objective of this study was assess the occurrence of reactive to antibodies anti-Leptospira spp., Brucella canis and B. abortus in Belém and Castanhal, State of Pará, Amazon, Brazil. A total of 156 samples were randomly collected in the city of Belém and 158 samples in Castanhal. The anti-B. canis antibodies research was performed by Agar Gel Immunodiffusion (AGID) with and without 2-mercaptoethanol serum treatment (AGID-2ME). To assess the anti-B. abortus antibodies, the technique of Fast Seroagglutination...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira spp.; Brucella spp.; Domestic canids; Serology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672015000300265

DETECTION OF INFECTION WITH Toxoplasma gondii IN MANATEES (Trichechus inunguis) OF THE PERUVIAN AMAZON
Provedor de dados: 37
Autores: DELGADO,PATRICK MATHEWS; PEREA,NOFRE SANCHEZ; MATHEWS DELGADO,JONH PAUL; GARCIA,CLAUDIA BIFFI; MALHEIROS,ANTONIO FRANCISCO; GARCIA DAVILA,CARMEN ROSA.
The Amazonian manatee (Trichechus inunguis) is an aquatic mammal that inhabits freshwater environments and is endemic to the Amazon Basin. The presence of Toxoplasma gondii antibodies was investigated in 19 manatees in one rescue unit in the northern region of Peru. Antibodies to T. gondii were detected in 12 (63.2 %) of 19 animals by using the modified agglutination test (titer, 1:25), and no association between sex and age of the animals and the presence of T. gondii antibodies was observed (p &lt; 0.05). The results suggest a contamination by T. gondii oocysts in the aquatic environment where these animals live.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazonian manatee; Peruvian Amazon; Serology; Toxoplasma gondii.
Ano: 2013
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2013000100017

Serum neutralization as a differential serological test for classical swine fever virus and other pestivirus infections
Provedor de dados: 45
Autores: Paredes,J.C.M.; Oliveira,E.A.S.; Oliveira,L.G.; Rosa,J.C.A.; Roehe,P.M.
Serum neutralization tests (SN) were performed against classical swine fever virus (CSFV), bovine viral diarrhea virus (BVDV) and border disease virus (BDV) on samples of swine serum collected for screening of antibodies to CSFV, in order to determine the SN value as a differential serological test. Ninety-nine sera out of a sample of 16,664 were positive for antibodies to pestiviruses in an ELISA test which did not distinguish antibodies to different pestiviruses. When submitted to SN, 81 sera were positive for CSFV antibodies only. In 17 sera, crossreactive antibodies to either CSFV, BVDV or BDV were detected. In most of these sera (13 out of 17) the differences between SN titres against the three viruses were not sufficient to estimate which was the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swine; Classical swine fever; Pestivirus; Serology.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000500001

Development and standardization of a western blotting test for detection of antibodies against B. abortus
Provedor de dados: 45
Autores: Falcão,M.V.D.; Santana,V.L.A.; Corrêa,F.N.; Tenório,J.A. B.; Mota,R.A.
ABSTRACT Brucellosis is an infectious disease caused by bacteria of the genus Brucella spp. with diagnosis based on use of serological techniques. The present study aimed to develop and standardize a western blotting (WB) test for detection of antibodies against B. abortus. Samples from two groups of cattle were analyzed: group I: 60 serum samples from true positive and true negative vaccinated animals (30 positive samples from infected animals according to rose bengal test (RBT), 2-mercaptoethanol, serum agglutination test (SAT) and complement fixation test (CFT) and 30 RBT negatives samples); group II: 383 field samples (90 positive and 293 CFT negative sera). The most reactive band in the western blotting, which properly identified and separated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brucellosis; Immunodiagnosis; Proteomics; Serology.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100160

Epidemiological assessment of canine brucellosis
Provedor de dados: 45
Autores: Megid,J.; Brito,A.F.; Moraes,C.C.G.; Fava,N.; Agottani,J.
Observou-se brucelose canina em quatro canis diferentes. Os canis apresentaram animais com história de aborto, mortalidade em neonatos e nascimentos prematuros. A porcentagem de animais soropositivos para brucelose canina, pela prova de imunodifusão em ágar gel, variou de 4,6 a 57,1%. Observou-se correlação positiva entre porcentagem de animais positivos e aspectos reprodutivos e condições de aglomeração.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Dog; Brucellosis; Epidemiology; Serology.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000500007

Antibody response between pigs of Piau and a commercial breed naturally infected with Porcine circovirus 2
Provedor de dados: 45
Autores: Bulos,L.H.S.; Gonzaga,N.F.; Souza,L.F.L.; Assao,V.S.; Leite,R.A.S.; Rebouças,M.S.; Scatamburlo,T.M.; Guimarães,J.D. Guimarães S.E.F.; Lopes,P.S.; Almeida,M.R.L.; Silva Júnior,A.
ABSTRACT Brazilian pig population is made up of several naturalized breeds; among them the Piau breed is known for its rusticity and large fat stores. The naturalized breeds, in comparison with commercial ones, may have an increased resistance to diseases circulating in their territory. Thus, this study aimed to verify if there are differences between the serologic profile against Porcine circovirus 2 (PCV2) of Piau pigs and that of a commercial breed from a farm naturally infected by PCV2. The serum viral load was measured by qPCR, and levels of anti-PCV2 antibodies were measured by ELISA. The results showed that the serum viral load was similar across all animals. However, Piau piglets showed higher levels of antibodies compared to commercial piglets (P=...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serum viral load; Serology; Piau.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501145

A comparison of two agar gel immunodiffusion methods and a complement fixation test for serologic diagnosis of Brucella ovis infection in experimentally infected rams
Provedor de dados: 45
Autores: Xavier,M.N.; Sant'Anna,F.M.; Silva,T.M.A.; Costa,E.A.; Moustacas,V.S.; Merlo,F.A.; Carvalho Júnior,C.A.; Dasso,M.G.; Mathias,L.A.; Gouveia,A.M.G.; Lage,A.P.; Santos,R.L.
A infecção por Brucella ovis é considerada uma das principais causas de epididimite e infertilidade em carneiros, resultando em falhas reprodutivas e perdas econômicas significativas em rebanhos ovinos ao redor do mundo. O estudo teve o objetivo de avaliar três testes sorológicos disponíveis para o diagnóstico da brucelose ovina por B. ovis, utilizando 181 soros ovinos. Amostras de soro provenientes de carneiros experimentalmente infectados foram coletadas ao longo de 192 dias pós-infecção (n=117) e durante o período pré-infecção (n=9). Adicionalmente, amostras de soro foram obtidas de ovinos provenientes de um rebanho livre para B. ovis (n=55). As técnicas de imunodifusão em gel de agar (IDGA), utilizando dois antígenos disponíveis comercialmente, e de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ram; Brucella ovis; Serology; Complement fixation; Immunodiffusion.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000400031

First molecular and serological detection of Epizootic Hemorrhagic Disease virus in white tailed deer ( Odocoileus virginianus ) from Tamaulipas, Mexico
Provedor de dados: 45
Autores: Merino,J.O.; Cruz,NI. De la; Galvan,G.; León,A.P. De; Burnes,J.
ABSTRACT Epizootic hemorrhagic disease viruses (EHDV) are dsRNA arboviruses transmitted by biting midges of the genus Culicoides that cause disease in domestic and wild ruminants. Epizootic hemorrhagic disease (EHD) is considered the most important infectious disease of white tailed deer (WTD) in North America, some studies in Northeast Mexico reported EHDV-seropositive WTD and EHDV-infected Culicoides vectors. The increasing population of WTD that share habitat with livestock in Northeast México highlights the importance of EHD for the livestock industry in the transboundary region with the U.S. One hundred and twenty two samples from WTD in Tamaulipas state, Mexico were tested by ELISA and RT-PCR for EHDV antibodies and nucleic acid, respectively. Twelve...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Deer; Detection; EHDV; RT-PCR; Serology.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100077

Natural infection of Viola cornuta (Violaceae) with Cucumber mosaic virus, subgroup I
Provedor de dados: 47
Autores: Arneodo,Joel; de Breuil,Soledad; Lenardon,Sergio; Conci,Luis.
Plants of Viola cornuta displaying typical virus symptoms were observed during spring 2003 in a plant nursery in Córdoba, central Argentina. Electron microscopic examinations of symptomatic leaf samples revealed the presence of isometric virus-like particles about 30 nm in diameter. Subsequent serological analysis allowed the identification of the pathogen as a subgroup I strain of Cucumber mosaic virus (CMV). These results were confirmed by antigen capture - reverse transcription - polymerase chain reaction with specific CMV primers, and digestion with a restriction enzyme. This is the first report of CMV infecting V. cornuta in Argentina.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Viola cornuta; Cucumber mosaic virus; Electron microscopy; Serology; AC-RT-PCR.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200008

Exposure of pampas fox (Pseudalopex gymnocercus) and crab-eating fox (Cerdocyon thous) from the Southern region of Brazil to Canine distemper virus (CDV), Canine parvovirus (CPV) and Canine coronavirus (CCoV)
Provedor de dados: 52
Autores: Hübner,Silvia de Oliveira; Pappen,Felipe Geraldes; Ruas,Jerônimo Lopes; Vargas,Gilberto D'Ávila; Fischer,Geferson; Vidor,Telmo.
The exposure of 13 Brazilian free-ranging nondomestic canids (five pampas fox - Pseudalopex gymnocercus and eight crab-eating fox -Cerdocyon thous) from Southern region of Brazil, to Canine distemper virus (CDV), canine parvovirus (CPV) and Canine coronavirus (CCoV) was investigated. Antibodies against CDV were detected in 38.5% (5/13) of the samples. There were anti-CDV antibodies in 60% (3/5) of P. gymnocercus and in 25% (2/8) of C. thous. The frequency was higher among the adults and males. Eleven canids (84.6%) presented antibodies against CPV, 80% (4/5) were from P. gymnocercus and 87.5% (7/8) were from C. thous. There was no difference in positivity rate against CPV between gender and age. Antibodies against CCoV were detected in 38.5% (5/13) of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudalopex gymnocercus; Cerdocyon thous; Canine distemper virus (CDV); Canine parvovirus (CPV); Canine coronavirus (CCoV); Serology.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300012

Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4
Provedor de dados: 52
Autores: Krüger,Ernesto Renato; Penha,Tânia Regina; Hummelgen,Fernanda Cristina; Agottani,Jorge Bacila; Reva,Demétrio; Gonçalves,Robson; Thomaz-Soccol,Vanete.
ABSTRACTThe aim of this work was to develop and evaluate an indirect enzyme-linked immunosorbent assay (ELISA) and made a serological screening for specific antibodies to BoHV-4. Bovine serum samples were collected from different Brazilian states and evaluated for the presence of antibodies for BoHV-4 and BoHV-1. The serological results obtained showed that the indirect ELISA assay could be applied for the detection of specific antibodies for BoHV-4. The ELISA test allowed concluding that BoHV-4 is present in bovines in every Brazilian state from which serum samples were collected. The ELISA assay here standardized proved to be useful for the epidemiological studies and showed a positivity range from 1.8 to 66% in Brazil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BoHV-4; Serology; Diagnostic; Cattle; Epidemiology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500725

Neurobrucellosis: clinical, diagnostic, therapeutic features and outcome. Unusual clinical presentations in an endemic region
Provedor de dados: 55
Autores: Ceran,Nurgul; Turkoglu,Recai; Erdem,Ilknur; Inan,Asuman; Engin,Derya; Tireli,Hulya; Goktas,Pasa.
Brucellosis is a zoonotic infection and has endemic characteristics. Neurobrucellosis is an uncommon complication of this infection. The aim of this study was to present unusual clinical manifestations and to discuss the management and outcome of a series of 18 neurobrucellosis cases. Initial clinical manifestations consist of pseudotumor cerebri in one case, white matter lesions and demyelinating syndrome in three cases, intracranial granuloma in one case, transverse myelitis in two cases, sagittal sinus thrombosis in one case, spinal arachnoiditis in one case, intracranial vasculitis in one case, in addition to meningitis in all cases. Eleven patients were male and seven were female. The most prevalent symptoms were headache (83%) and fever (44%). All...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurobrucellosis; Serology; Diagnosis; Neuroimaging.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100010

Clinical significance of different bacterial load ofMycoplasma pneumoniae in patients with Mycoplasma pneumoniae pneumonia
Provedor de dados: 55
Autores: Jiang,Wujun; Yan,Yongdong; Ji,Wei; Wang,Yuqing; Chen,Zhengrong.
OBJECTIVE:This retrospective study was conducted to investigate the clinical significance of differentMycoplasma pneumoniae bacterial load in patients with M. pneumoniae pneumonia (MP) in children.METHODS:Patients with MP (n=511) were identified at the Children's Hospital Affiliated to Soochow University database during an outbreak of MP between January 2012 and February 2013.RESULTS:Comparing patients with high and low bacterial load those with higher loads were significantly older (p&lt;0.01) and had fever significantly more frequently (p=0.01). Presence of wheezing at presentation was associated with low bacterial load (p=0.03). Baseline positive IgM was present in 93 (56.4%) patients with high bacterial load compared to 46 (27.8%) patients with low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma pneumoniae; Pneumonia; Polymerase chain reaction; Serology.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000200124

First survey for detecting the presence of human herpesvirus 8 infection (HHV-8) in Maputo, Mozambique
Provedor de dados: 55
Autores: Caterino-de-Araujo,Adele; Manuel,Rolanda Carmen Rafael; Bianco,Rosana Del; Santos-Fortuna,Elizabeth; Magri,Mariana Cavalheiro; Silva,Joyce Matie Kinoshita; Bastos,Rui.
Human herpesvirus 8 (HHV-8), the etiological agent of Kaposi's sarcoma (KS), is endemic in parts of the sub-Saharan, and KS has increased concomitantly with the HIV/AIDS epidemic. In Mozambique (MZ), no data concerning HHV-8 infection was available, thus the main of this work was to investigate, for the first time, the presence of HHV-8 infection in Maputo, MZ. Latent and lytic HHV-8-specific antibodies were assessed in blood samples from 189 individuals from the Central Hospital of Maputo, MZ, using "in-house" immunofluorescence assays conducted in São Paulo, Brazil. The results obtained were analyzed according to socio-demographic and clinical variables using the Chi-square test and logistic regression. An HHV-8 seropositivity of 1.8% and 9.7% was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human herpesvirus 8 (HHV-8); Serology; Mozambique; Frequency; Kaposi's sarcoma.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000300008

Latent human herpesvirus - 8 (HHV-8) infection in female commercial sex workers from Imbituba, Santa Catarina, Brazil
Provedor de dados: 55
Autores: Caterino-de-Araujo,Adele; Santos-Fortuna,Elizabeth; Magri,Mariana Cavalheiro; Schuelter-Trevisol,Fabiana; Silva,Marcos Vinicius.
Human herpesvirus 8 (HHV-8) infection was identified in 6 out of 90 (6.7%) female commercial sex workers from Imbituba, Santa Catarina, and was associated to age. Frequencies of 5.6% of anti-latent and 3.3% of anti-lytic antibodies were detected. Considering non-endemic areas from Brazil, the anti-latent antibodies frequency seems elevated and requires further investigation on referent female population.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HHV-8; Serology; Anti-latent antibodies; Female sex workers.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100004

Dengue: a review of the laboratory tests a clinician must know to achieve a correct diagnosis
Provedor de dados: 55
Autores: De Paula,Sérgio Oliveira; Fonseca,Benedito Antônio Lopes da.
Dengue is the most important disease caused by an arbovirus (1, 2, 3 and 4 serotypes) worldwide, especially in the tropical and sub-tropical regions. Its clinical manifestations range from asymptomatic infections to a severe disease characterized by hemorrhage and shock. The incidence of dengue virus activity in the Americas has substantially increased from 1980 to 1994. In Brazil, the increase in the incidence of dengue is especially linked to the dissemination of Aedes aegypti. Thus, a rapid and accurate dengue diagnosis is of paramount importance for effective control of dengue outbreaks [8]. Five serological tests have been used for the diagnosis of dengue infection: hemagglutination-inhibition (HI), complement fixation (CF), neutralization test (NT),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dengue; Diagnosis; Serology; PCR.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000600002

Bartonella native valve endocarditis: the first brazilian case alive and well
Provedor de dados: 55
Autores: Lamas,C.; Favacho,A.; Ramos,R.G.; Santos,M.S.; Ferravoli,G.I.; Weksler,C.; Rozental,T.; Bóia,M.N.; Lemos,E.R.S.
Bartonella is an important cause of blood culture-negative endocarditis in recent studies. Seroprevalence studies in the States of Minas Gerais and Rio de Janeiro have shown Bartonella IgG positivity around 14% in healthy adults and 40% in HIV seropositive adults, respectively. A case report of a 46-year-old white male with moderate aortic regurgitation (AR) due to rheumatic heart disease (RHD), admitted due to worsening heart failure, is presented. Clinical features were apyrexia, anemia, polyclonal hypergammaglobulinemia, hematuria and splenomegaly. He was submitted to surgery due to worsening AR. Histopathology of the excised valve showed active bacterial endocarditis and underlying RHD. Routine blood cultures were negative. Indirect immunofluorescence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bartonella spp.; Infective endocarditis; Blood culture-negative infective endocarditis; Rheumatic heart disease; Serology; Indirect immunofluorescence.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000600012

Genital schistosomiasis mansoni concomitant to genital tumor in areas of low endemicity: challenging diagnosis
Provedor de dados: 55
Autores: Cavalcanti,Marta G; Gonçalves,Margareth MI; Barreto,Magali M; Silva,Aline Helen da; Madi,Kalil; Peralta,José Mauro; Igreja,Ricardo P.
Genital infection by Schistosoma mansoni is usually misdiagnosed in individuals who reside in, or travel to endemic areas. We describe two cases of genital tumor associated with S. mansoni infection manifested by methrorragy. Surgical specimens revealed leiomyomas in both cases associated with S. mansoni. In one of them, granulomas were found in the ovary and in the other they were found in the uterine tube. Although none presented intestinal/hepatic disease, fecal egg excretion was detected in one. Both had elevated pretreatment antibody reactivity to S. mansoni antigen, but follow-up showed different outcomes. Schistosomiasis should be considered as a diagnosis in individuals with methrorragy residing in or having traveled to endemic areas. Since...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: S. mansoni; Female genital infection; Serology; Low endemic area.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200016

Improvement of an in-house ELISA for bovine paratuberculosis serology in Brazil
Provedor de dados: 58
Autores: Marassi,Carla Dray; Fonseca,Leila de Souza; Ristow,Paula; Ferreira,Rachel; Lilenbaum,Walter; Oelemann,Walter M.R.
Paratuberculosis (Johne's disease) is a chronic enteritis in cattle caused by infection with Mycobacterium avium subsp. paratuberculosis (Map). In Brazil, few reports describe the isolation of the organism. The gold standard test for Map is its isolation from tissues or feces. Moreover, bacterial growth is slow and test results are available only after three to five months of incubation Furthermore, shedding of bacilli at levels detectable by fecal culture is irregular and does not occur during the early stages of infection, which compromises the sensitivity of this methodology. The most common immunological tests to identify Map infection are complement fixation test (CFT), agar gel immunodiffusion (AGID) and enzyme-linked immunoassay (ELISA). In Brazil,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paratuberculosis; Cattle; ELISA; Serology; Diagnosis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200004

Mycoplasma synoviae infection on Newcastle disease vaccination of chickens
Provedor de dados: 58
Autores: Silva,Rita de Cássia Figueira; Nascimento,Elmiro Rosendo do; Pereira,Virgínia Léo de Almeida; Barreto,Maria Lúcia; Nascimento,Maria da Graça Fichel do.
Newcastle disease is characterized by respiratory manifestations in association with nervous and/or digestive symptoms. Its prevention is done by vaccination with live attenuated (lentogenic strains) and/or killed vaccines. The lentogenic strains can lead to strong post-vaccination reaction, principally due to the presence of other pathogenic agents. Among them, Mycoplasma synoviae is worldwide important, mainly in Brazil. The dissemination of this agent in poultry flocks has been achieved due to difficulties in diagnosis and disease reproduction, virulence variations among different M.synoviae strains, and attribution of typical M.synoviae disease manifestation to other disease agents. This experimental study in SPF chicks (Gallus gallus), previously...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vaccine; Serology; Mycoplasma; Newcastle disease.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200033

Experimental infection with Brazilian Newcastle disease virus strain in pigeons and chickens
Provedor de dados: 58
Autores: Carrasco,Adriano de Oliveira Torres; Seki,Meire Christina; Benevenute,Jyan Lucas; Ikeda,Priscila; Pinto,Aramis Augusto.
Abstract This study was designed with the goal of adding as much information as possible about the role of pigeons (Columba livia) and chickens (Gallus gallus) in Newcastle disease virus epidemiology. These species were submitted to direct experimental infection with Newcastle disease virus to evaluate interspecies transmission and virus-host relationships. The results obtained in four experimental models were analyzed by hemagglutination inhibition and reverse transcriptase polymerase chain reaction for detection of virus shedding. These techniques revealed that both avian species, when previously immunized with a low pathogenic Newcastle disease virus strain (LaSota), developed high antibody titers that significantly reduced virus shedding after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gallus gallus; Columba livia; Experimental infection; RT-PCR; Serology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100231

Rapid polyvalent screening for largescale environmental Spiroplasma surveys
Provedor de dados: 58
Autores: French,Frank E.; Whitcomb,Robert F.; Williamson,David L.; Regassa,Laura B.
Surface serology is an important determinant in Spiroplasma systematics. Reciprocal antigen/antibody reactions between spiroplasmas and individual antisera delineate the 38 described groups and species. However, reciprocal serology is impractical for largescale studies. This report describes a successful, streamlined polyvalent screening approach used to examine isolates from an environmental survey.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spiroplasma; Mollicutes; Serology; Deformation test.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300031

Comparison of an in-house and a commercial enzyme-linked immunosorbent assay (ELISA) for diagnosis of paratuberculosis
Provedor de dados: 58
Autores: Marassi,Carla Dray; Gonzaga,Janete da Silva; Ristow,Paula; Ferreira,Rachel; Fonseca,Leila Souza; Oelemann,Walter; Lilenbaum,Walter.
An in-house PPA-ELISA was compared to a commercial ELISA using a panel of 108 serum samples. In relation to commercial test, in-house assay presented 100% of sensitivity and 83.5% of specificity and a high concordance (kappa&gt; 0.5), which demonstrates that the in-house assay is a valuable tool for use in developing countries.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Mycobacterium avium subsp. paratuberculosis; Serology.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100002

Diagnosis of histoplasmosis
Provedor de dados: 58
Autores: Guimarães,Allan Jefferson; Nosanchuk,Joshua D.; Zancopé-Oliveira,Rosely Maria.
Endemic mycoses can be challenging to diagnose and accurate interpretation of laboratory data is important to ensure the most appropriate treatment for the patients. Although the definitive diagnosis of histoplasmosis (HP), one of the most frequent endemic mycoses in the world, is achieved by direct diagnosis performed by micro and/or macroscopic observation of Histoplasma capsulatum (H. capsulatum), serologic evidence of this fungal infection is important since the isolation of the etiologic agents is time-consuming and insensitive. A variety of immunoassays have been used to detect specific antibodies to H. capsulatum. The most applied technique for antibody detection is immunodiffusion with sensitivity between 70 to 100 % and specificity of 100%,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnostic tests; Culture based methods; Molecular methods; Serology.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100001

Detection of Mycoplasma pulmonis in laboratory rats
Provedor de dados: 58
Autores: Barreto,Maria Lucia; Nascimento,Elmiro Rosendo do; Campos,Carlos Augusto de Martino; Nascimento,Maria da Graça Fichel do; Lignon,Gilberto Brasil; Lira,Marie Luce Flores; Silva,Ricardo G.
This work was conducted on rats in two premises located in Niterói and Rio de Janeiro, Brazil. One is classified as conventional controlled and the other, conventional. The objective of the present study was to detect the presence of Mycoplasma pulmonis in animals with symptoms of respiratory disease and low reproductive performance. In the conventional controlled premises, 16 rats of Wistar-Furth strain were necropsied while in the conventional premises necropsy was performed on 12 rats of Hooded Lister strain. The clinical samples of lungs, trachea, oropharynx, middle ear, uterus and ovaries were subjected to culturing while the sera were tested for antibody detection. From 28 rats, 57.14% (16/28) were culture positive for M. pulmonis, being 81.25%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma pulmonis; Diagnosis; Isolation; Serology; Rats.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300015

Seroepidemiology ofLeishmaniaspp. in equids from Uberlândia, Minas Gerais, Brazil
Provedor de dados: 65
Autores: Oliveira,Patrícia Magalhães de; Garcia,Felipe; Evers,Fernanda; Barbosa,Vinícius de Morais; Obando,Diego Camilo Mora; Nasciutti,Nayara Resende; Garcia,João Luis; Nogueira,Geison Morel; Headley,Selwyn Arlington; Saut,João Paulo Elsen.
ABSTRACT: This study evaluated the occurrence of Leishmania spp. in equids from Uberlândia, Minas Gerais, Southeastern Brazil. Serum samples from 257 healthy equids of Uberlândia were assessed for the presence of antibodies against Leishmania spp. by using the indirect fluorescent-antibody test. Additionally, an epidemiological survey was done to evaluate the possible risk factors (species of equids, age, gender, economic activity, and contact with domestic and wild animals) associated with the presence of antibodies against Leishmania spp. The total seroprevalence against Leishmania spp. was 24.1% (62/257); representing seroprevalence levels of 22.7% (55/242) in horses, 50% (4/8) in mules, and 42.9% (3/7) in ponies from all regions evaluated. The species...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Equids; Serology; Epidemiology.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000500501

Leptospirosis in native mixed-breed sheep slaughtered in a semiarid region of Brazil
Provedor de dados: 65
Autores: Costa,Diego Figueiredo da; Silva,Aline Ferreira da; Brasil,Arthur Willian de Lima; Loureiro,Ana Paula Pereira; Santos,Fabrine Alexandre dos; Azevedo,Sergio Santos de; Lilenbaum,Walter; Alves,Clebert José.
ABSTRACT: Sheep breeding is an economic activity of great value for the Brazilian livestock industry, and infectious diseases can significantly compromise its productivity. The aim of this study was to detect Leptospira spp. in mixed breed sheep from a semiarid region of the State of Paraíba, Northeast Brazil. In total, 49 blood and renal tissue samples, as well as 25 urine samples, were collected from 49 sheep in the slaughter line. The microscopic agglutination test (MAT) was used to verify the presence of anti -Leptospira spp. antibodies in blood samples. Renal tissue and urine samples were analyzed by both PCR and bacterial culture. Sero reactivity was observed in 8.2% (4/49) of animals. Three renal samples were positive for leptospiral DNA by PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Small ruminants; PCR; Serology; Isolation; Slaughterhouse.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000200451

Antibodies against vesicular stomatitis virus in horses from southern, midwestern and northeastern Brazilian States
Provedor de dados: 65
Autores: Lunkes,Vinícius Leobet; Tonin,Alexandre Alberto; Machado,Gustavo; Corbellini,Luis Gustavo; Diehl,Gustavo Nogueira; Santos,Lucila Carboneiro dos; Bezerra,Camila de Sousa; Azevedo,Sérgio Santos de; Pequeno,Nebson Fernandes; Silva,Adriana Moraes da; Weiblen,Rudi; Flores,Eduardo Furtado.
ABSTRACT: Vesicular stomatitis virus (VSV) is the agent of a vesicular disease that affects many animal species and may be clinically confounded with foot-and-mouth disease in ruminant and swine. Horses are especially susceptible to VSV and may serve as sentinels for virus circulation. The present study investigated the presence of neutralizing antibodies against VSV Indiana III (VSIV-3) in serum samples of 3,626 horses from six states in three Brazilian regions: Southern (RS, n = 1,011), Midwest (GO/DF, n = 1,767) and Northeast (PB, PE, RN and CE, n = 848) collected between 2013 and 2014. Neutralizing antibodies against VSIV-3 (titers ≥40) were detected in 641 samples (positivity of 17.7%; CI95%:16.5-19.0%), being 317 samples from CE (87.3%; CI95%:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serology; Differential diagnosis; Foot-and-mouth disease; Zoonosis; Vesicular disease.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000801424

Toxoplasmosis in squirrel monkeys: histological and immunohistochemical analysis
Provedor de dados: 65
Autores: Andrade,Márcia Cristina Ribeiro; Coelho,Janice Mery Chicarino de Oliveira; Amendoeira,Maria Regina Reis; Vicente,Regiane Trigueiro; Cardoso,Célia Virgínia Pereira; Ferreira,Paola Cristina Brandão; Marchevsky,Renato Sergio.
Necropsy findings in three cases of naturally occurring toxoplasmosis in squirrel monkeys (Saimiri sciureus) obtained as wild catches from the Amazon region were described. Histopathological evaluation of the liver, spleen, kidneys and mesenteric lymph nodes showed multiple foci of inflammation and necrosis. Typical, well-defined morphologic Toxoplasma gondii cysts and free forms of the parasite were found by immunoperoxidase staining within inflammatory infiltrates. The presence of T. gondii in the studied colony could severely influence the results of experiments performed in the animals.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Squirrel monkeys; Neotropical primates; Toxoplasmosis; Immunohistochemical analysis; Serology.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000600034

Immunogenicity in sheep of Uruguayan commercial vaccines against bovine alphaherpesvirus 1, 5 and bovine pestiviruses
Provedor de dados: 65
Autores: Merchioratto,Ingryd; Aurélio,Alana de Almeida; Villela,Janice Machado; Stone,Nicole Vieira; Roman,Isac Junior; Traesel,Carolina Kist; Brum,Mário Celso Sperotto.
ABSTRACT: The serological responses induced by four commercial inactivated Uruguayan vaccines against bovine alphaherpesviruses (BoHV)-1 and -5 and bovine pestiviruses (BVDV-1, BVDV-2, and HoBiPeV) were evaluated in sheep. Thirty-seven sheep were immunized twice (day 0 and 25) and their serum samples were tested at different intervals (days 0, 25, 40, 60, and 90) post-vaccination (PV). Among the four vaccines tested, only one (G4) could induce the production of moderate neutralizing antibody titers against BoHV-1 and -5 and BVDV-1 and -2. The G3 vaccine showed a neutralizing serological response against the bovine alphaherpesviruses only. The G1 and G2 vaccines produced extremely low levels of antibodies in a few vaccinated animals only (geometric mean...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serology; Neutralizing antibodies; Vaccine response; Reproductive disease.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000400454