Consulta: subjectFacets:"Serum and stool"
Registros recuperados: 1
Data/hora: 07/06/2024 09:21:53

Comparison of four extraction methods to detect hepatitis A virus RNA in serum and stool samples
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Paula,Vanessa Salete de; Villar,Livia Melo; Gaspar,Ana Maria Coimbra.
The efficiency of extraction methods for hepatitis A virus (HAV) RNA in clinical samples is of great importance for molecular diagnosis, especially in regions endemic for HAV, such as Brazil. We compared the efficiency of four different extraction techniques in serum and stool samples for the detection of hepatitis A virus by reverse transcription PCR (RT-PCR). We used PCR to analyse serum and stool samples of 12 patients who were referred to the Brazilian Reference Center for Viral Hepatitis (BRCVH) in Rio de Janeiro. The methods tested were Proteinase K, Silica, TRIzol and Guanidine isothiocyanate. Proteinase K extraction was the best method for serum samples; it detected the HAV-RNA in 11 of the 12 samples. The guanidine isothiocyanate method was the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HAV; Extraction methods; Serum and stool; RT-PCR.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000200007