Consulta: subjectFacets:"Sirex Noctilio Extração de DNA"
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Data/hora: 11/06/2024 19:16:30

Isolamento de DNA genômico de indivíduo de nematoide parasita de insetos: estudo de caso de Deladenus
Provedor de dados: 190
Autores: Schuhli, Guilherme Schnell e; Penteado, Susete do Rocio Chiarello; Kestring, Daiane Rigoni; Steil, Gisleine Jarenko; Thomas, Micheli Cristina.
Populational studies involve single individual DNA extraction in order to grant further genotyping data. In the case of some parasitic nematodes, the reduced dimensions and high individual number per infestation makes individual genotyping a difficult task. Aiming the development of a protocol we performed adjustments in available methods in order to acquire the best gain in purity and concentration of genomic DNA. Single specimens were digested in Worm Lysis Buffer and submitted to PCR amplification as a concept test. It was possible to obtain good amount and concentration of DNA from individuals. Quality was sufficient to grant subsequent ITS1 sequencing.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genética Molecular; Entomologia Florestal; Controle Biológico DNA extraction; Protocol; Woodwasp; Sirex Noctilio Extração de DNA; Protocolo; Vespa-da-madeira; Sirex Noctilio.
Ano: 2012
URL: http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/396