Consulta: subjectFacets:"Small RNA"
Registros recuperados: 2
Data/hora: 10/06/2024 11:10:46

Metatranscriptomic analysis of small RNAs present in soybean deep sequencing libraries
Provedor de dados: Genetics and Molecular Biology
Autores: Molina,Lorrayne Gomes; Cordenonsi da Fonseca,Guilherme; Morais,Guilherme Loss de; de Oliveira,Luiz Felipe Valter; Carvalho,Joseane Biso de; Kulcheski,Franceli Rodrigues; Margis,Rogerio.
A large number of small RNAs unrelated to the soybean genome were identified after deep sequencing of soybean small RNA libraries. A metatranscriptomic analysis was carried out to identify the origin of these sequences. Comparative analyses of small interference RNAs (siRNAs) present in samples collected in open areas corresponding to soybean field plantations and samples from soybean cultivated in greenhouses under a controlled environment were made. Different pathogenic, symbiotic and free-living organisms were identified from samples of both growth systems. They included viruses, bacteria and different groups of fungi. This approach can be useful not only to identify potentially unknown pathogens and pests, but also to understand the relations that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Next generation sequencing; Small RNA; SiRNA; Molecular markers.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200010

Salt stress affects mRNA editing in soybean chloroplasts
Provedor de dados: Genetics and Molecular Biology
Autores: Rodrigues,Nureyev F.; Fonseca,Guilherme C. da; Kulcheski,Franceli R.; Margis,Rogério.
Abstract Soybean, a crop known by its economic and nutritional importance, has been the subject of several studies that assess the impact and the effective plant responses to abiotic stresses. Salt stress is one of the main environmental stresses and negatively impacts crop growth and yield. In this work, the RNA editing process in the chloroplast of soybean plants was evaluated in response to a salt stress. Bioinformatics approach using sRNA and mRNA libraries were employed to detect specific sites showing differences in editing efficiency. RT-qPCR was used to measure editing efficiency at selected sites. We observed that transcripts of NDHA, NDHB, RPS14 and RPS16 genes presented differences in coverage and editing rates between control and salt-treated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Small RNA; Chloroplast; RNA editing; PPR; Salt stress.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200200