Consulta: subjectFacets:"Snake venoms"
Registros recuperados: 12
Data/hora: 08/06/2024 03:34:45

POLYACRYLAMIDE GEL ELECTROPHORESIS AS A TOOL FOR THE TAXONOMIC IDENTIFICATION OF SNAKES FROM THE ELAPIDAE AND VIPERIDAE FAMILIES
Provedor de dados: Journal of Venomous Animals and Toxins
Autores: SOARES,A. M.; ANZALONI PEDROSA,L. H.; FONTES,M. R. M.; DA SILVA,R. J.; GIGLIO,J. R.
Polyacrylamide gel electrophoresis (PAGE) for basic proteins may be a useful tool for the characterization of whole snake venoms and for the taxonomic classification of snakes of the Elapidae and Viperidae families. However, due to the close proximity of molecular weights among toxins bearing different net charges, sodium dodecyl sulfate (SDS) - PAGE was not able to provide an efficient differentiation. This article reports the electrophoretic analysis of several venoms from the genera Micrurus, Bothrops, Bothriopsis, Crotalus and Lachesis and shows a typical and distinctive electrophoretic profile for each species, with intraspecific and geographic variations. Even in cases in which extreme morphological similarities were present, such as between B....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Snake venoms; Basic toxins; Polyacrylamide gel electrophoresis; Elapidae; Viperidae.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301998000200005

USE OF FIBRIN GLUE DERIVED FROM SNAKE VENOM IN TESTICULAR BIOPSY OF RAMS
Provedor de dados: Journal of Venomous Animals and Toxins
Autores: SARTORI FILHO,R.; PRESTES,N. C.; THOMAZINI,I. A.; MENDES-GIANNINI,M. J.; TOSCANO,E.; CANAVESSI,A. M. O.; BARRAVIERA,B.
Sequelae due to testicular biopsy such as hemorrhage, adhesion and fibrosis may be limiting factors to the use of this surgical procedure. Fibrin glue (FG) derived from snake venom was used to minimize these sequelae, as well as to evaluate its healing property in tunica vaginalis and scrotal skin of rams. Applicability of fibrin glue derived from snake venom was tested in different tissues of other animals such as in sciatic nerve and colon of rats and skin of rabbits. In the present study, 30 healthy adult rams were used. They were divided into 3 groups of 10 animals each as follows: G1: fibrin glue group (application of fibrin glue on puncture sites and skin incisions after bilateral testicular biopsy with a Tru-Cut needle); G2: swab/nylon group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibrin tecidual adhesive; Snake venoms; Biopsy; Testicle; Ovine.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301998000100003

A SENSITIVE AND SPECIFIC IMMUNOASSAY FOR THE MEASUREMENT OF THE ANTIBODIES PRESENT IN HORSE ANTIVENOMS ENDOWED WITH THE CAPACITY TO BLOCK THE PHOSPHOLIPASE A2-DEPENDENT HEMOLYSIS INDUCED BY SNAKE VENOMS
Provedor de dados: Journal of Venomous Animals and Toxins
Autores: ROCHA CAMPOS,A. C. M.; FREITAS,M. C. W. DE; YAMAGUCHI,I. K.; MARCELINO,J. R.; DIAS DA SILVA,W.
Phospholipase A2 (PLA2), a component of most snake venom toxins, cleaves 3-sn-phosphoglycerides releasing lysophosphatidyl-choline. The indirect quantitative assay method for PLA2 was standardized for specific antivenom titration in a fast and sensitive assay by the similarity with the hemolysis induced by PLA2 and by complement system in sheep erythrocytes. The curves obtained by plotting the degree of hemolysis against the doses of snake venom are concave to the abscissa axis following an equation similar to that previously described for the hemolysis induced by the C system. We observed that venoms of some Bothrops, Crotalus and Micrurus species contained around 1 x 10&lt;img SRC="http:/img/fbpe/jvat/v2n2/image603.gif"&gt; to 10&lt;img...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PLA2; Snake venoms; Immunoassay; Antivenoms; Indirect hemolysis.
Ano: 1996
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301996000200004

Strategies in ‘snake venomics’ aiming at an integrative view of compositional, functional, and immunological characteristics of venoms
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Lomonte,Bruno; Calvete,Juan J.
Abstract This work offers a general overview on the evolving strategies for the proteomic analysis of snake venoms, and discusses how these may be combined through diverse experimental approaches with the goal of achieving a more comprehensive knowledge on the compositional, toxic, and immunological characteristics of venoms. Some recent developments in this field are summarized, highlighting how strategies have evolved from the mere cataloguing of venom components (proteomics/venomics), to a broader exploration of their immunological (antivenomics) and functional (toxicovenomics) characteristics. Altogether, the combination of these complementary strategies is helping to build a wider, more integrative view of the life-threatening protein cocktails...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venoms; Proteomics; Venomics; Antivenomics; Toxicovenomics.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100206

Antivenomic approach of different Crotalus durissus collilineatus venoms
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Oliveira,Isadora Sousa de; Pucca,Manuela Berto; Sampaio,Suely Vilela; Arantes,Eliane Candiani.
Abstract  Background: Our group has previously performed a proteomic study verifying that individual variations can occur among Crotalus durissus collilineatus venoms. These variations may lead to differences in venom toxicity and may result in lack of neutralization of some components by antivenom. In this way, this study aimed to evaluate the Brazilian anticrotalic serum capacity in recognizing twenty-two Crotalus durissus collilineatus venoms, as well as their fractions.  Methods: The indirect enzyme-linked immunosorbent assay (ELISA) was chosen to evaluate the efficacy of heterologous anticrotalic serum produced by Instituto Butantan (Brazil) in recognizing the twenty-two Crotalus durissus collilineatus venoms and the pool of them. Moreover, the venom...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Snake venoms; Crotalus durissus collilineatus; Antivenomic; Crotalic antivenom; Individual variation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100601

Purification procedure for the isolation of a P-I metalloprotease and an acidic phospholipase A 2 fromBothrops atrox snake venom
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Menaldo,Danilo L.; Jacob-Ferreira,Anna L.; Bernardes,Carolina P.; Cintra,Adélia C. O.; Sampaio,Suely V.
Background Snake venoms are complex mixtures of inorganic and organic components, mainly proteins and peptides. Standardization of methods for isolating bioactive molecules from snake venoms is extremely difficult due to the complex and highly variable composition of venoms, which can be influenced by factors such as age and geographic location of the specimen. Therefore, this study aimed to standardize a simple purification methodology for obtaining a P-I class metalloprotease (MP) and an acidic phospholipase A2 (PLA 2 ) from Bothrops atroxvenom, and biochemically characterize these molecules to enable future functional studies.Methods To obtain the toxins of interest, a method has been standardized using consecutive isolation steps. The purity level of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venoms; Bothrops atrox; Toxins; Metalloprotease; Phospholipase A2; Isolation; Characterization; Chromatography.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100337

Dangerous snakes, deadly snakes and medically important snakes
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Silva,Anjana.
This correspondence argues that the dangerousness of a venomous snake species is not solely determined by the venom characteristics or the lethality of the snake, and recognizes that medical importance comprises a key variable as well. The medical importance of a snake is determined by several factors - including frequency of medical attention after a bite, local or systemic envenomation provoked by the bite, fatal bites, long term consequences, availability of antivenom therapy as well as the size of the population at risk - that may vary from one region to another.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Snakes; Snake bites; Snake venoms; Snake envenomation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100102

Bothrops jararacussu venom-induced neuromuscular blockade inhibited by Casearia gossypiosperma Briquet hydroalcoholic extract
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Camargo,TM; Nazato,VS; Silva,MG; Cogo,JC; Groppo,FC; Oshima-Franco,Y.
The hydroalcoholic extract of Casearia gossypiosperma Briquet (Flacourtiaceae) was standardized for the first time through quality control procedures including pharmacognostic methods, fingerprint chromatograms, defined amounts of marker substances and physicochemical characteristics. The pharmacological activity of C. gossypiosperma (Cg) hydroalcoholic extract was assayed by a traditional in vitro test, which involved irreversible neuromuscular blockade induced by Bothrops jararacussu (Bjssu) venom (60 µg/mL) in mouse phrenic nerve-diaphragm preparations. Bjssu venom blocked muscle activity for 26 (± 2.0) minutes (n = 6). Cg extract (0.1 mg/mL) induced changes on the baseline muscle activity without impairing the muscle function and inhibited 87.6% (±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibothropic extract; Bothrops jararacussu; Casearia gossypiosperma Briquet; Snake venoms.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300009

Snake venom L-amino acid oxidases: an overview on their antitumor effects
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Costa,Tássia R; Burin,Sandra M; Menaldo,Danilo L; Castro,Fabíola A de; Sampaio,Suely V.
The L-amino acid oxidases (LAAOs) constitute a major component of snake venoms and have been widely studied due to their widespread presence and various effects, such as apoptosis induction, cytotoxicity, induction and/or inhibition of platelet aggregation, hemorrhage, hemolysis, edema, as well as antimicrobial, antiparasitic and anti-HIV activities. The isolated and characterized snake venom LAAOs have become important research targets due to their potential biotechnological applications in pursuit for new drugs of interest in the scientific and medical fields. The current study discusses the antitumor effects of snake venom LAAOs described in the literature to date, highlighting the mechanisms of apoptosis induction proposed for this class of proteins.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venoms; L-amino acid oxidases; Antitumor effects; Apoptosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200206

Comparative analysis of the venom proteome of four important Malaysian snake species
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Vejayan,Jaya; Khoon,Too Lay; Ibrahim,Halijah.
BackgroundNaja kaouthia, Ophiophagus hannah, Bungarus fasciatusandCalloselasma rhodostomaare four venomous snakes indigenous to Malaysia. In the present study, their proteomic profile by two-dimensional gel electrophoresis (2-DE) have been separated and compared.ResultsThe 2-DE of venoms of the four species snake demonstrated complexity and obvious interspecies differences in proteome profiles. A total of 63 proteins were identified in the four species: C. rhodostoma -26, N. kaouthia -16, O. hannah -15 and B. fasciatus -6.ConclusionsDespite the identifications of major proteins in the four snake species, a large number of protein spots from the 2-DE were unidentified even though the spots displayed high-quality MALDI-TOF-MS spectra. Those identified...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mass spectrometry; Snake venoms; Proteomics; Two-dimensional gel electrophoresis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200317

What is the most dangerous snake?
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: McCue,Marshall D.
Letter to Editor of Journal of Venomous Animals and Toxins.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Snakes; Snake bites; Snake venoms; Snake envenomation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100101

Histological and biochemical effects induced by sublethal doses of Bothrops jararacussu venom in mice
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Zeni,A. L. B.; Becker,A.; Krug,M.; Albuquerque,C. A. C.
Snake venom is characterized by hemorrhagic, coagulant, proteolytic and myotoxic activities which in Bothrops jaracussu venom are related to intraspecific variations. In the present study, female Swiss mice were divided into two groups: treated with 25µg or 50µg venom. These were subdivided into three groups of six animals each, according to blood collection: 2, 4 or 24h after venom injection. Animals were anesthetized using diethyl-ether inhalation and 1ml of blood was collected by heart puncture. Then, the following organs were removed: spleen, skeletal muscle, kidneys, liver and lungs; histological sections were obtained and stained with hematoxylin-eosin (HE). The following biochemical parameters were analyzed: aspartate aminotransferase (AST/GOT),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararacussu; Snake venoms; Sublethal doses; In vivo studies; Biochemical parameters; Histopathological alterations.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300009